Restriction map for
pFN21ASDA0782
(Circular) (Six-base) MAPSORT of: pFN21ASDA0782.seq Check: 9998 from: 1 to: 8344
pFN21ASDA0782 8344 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2364 5363 2364
Size: 2999 5345
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6154 278
Size: 53 83 186 936 4618 2468
Fragments arranged by size:
4618 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2885 5319 5637 5687 1937
Size: 948 2434 318 50 4594
Fragments arranged by size:
4594 2434 948 318 50
Acc65I G'GTAC_C
Cuts at: 4485 5409 5665 4485
Size: 924 256 7164
Fragments arranged by size:
7164 924 256
AclI AA'CG_TT
Cuts at: 1563 6471 6844 1563
Size: 4908 373 3063
Fragments arranged by size:
4908 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2791 3077 6407 7791 8059 1632
Size: 1159 286 3330 1384 268 1917
Fragments arranged by size:
3330 1917 1384 1159 286 268
AfeI AGC'GCT
Cuts at: 2396 2575 3002 4385 2396
Size: 179 427 1383 6355
Fragments arranged by size:
6355 1383 427 179
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8147
AflIII A'CryG_T
Cuts at: 1203 5506 7243 8220 1203
Size: 4303 1737 977 1327
Fragments arranged by size:
4303 1737 1327 977
AgeI A'CCGG_T
Cuts at: 7175 7175
Size: 8344
AhdI GACnn_n'nnGTC
Cuts at: 4165 5617 7073 4165
Size: 1452 1456 5436
Fragments arranged by size:
5436 1456 1452
AleI CACnn'nnGTG
Cuts at: 1166 3455 5372 1166
Size: 2289 1917 4138
Fragments arranged by size:
4138 2289 1917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5234 1184
Size: 4050 4294
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3920 3952 3920
Size: 32 8312
AlwNI CAG_nnn'CTG
Cuts at: 2527 2934 3418 4054 4297 4847 5214 5525 6087
Size: 407 484 636 243 550 367 311 562
Cuts at: 6087 7659 2527
Size: 1572 3212
Fragments arranged by size:
3212 1572 636 562 550 484 407 367
311 243
ApaI G_GGCC'C
Cuts at: 2563 3583 3925 3984 4623 5266 2563
Size: 1020 342 59 639 643 5641
Fragments arranged by size:
5641 1020 643 639 342 59
ApaLI G'TGCA_C
Cuts at: 4331 4595 6401 7557 4331
Size: 264 1806 1156 5118
Fragments arranged by size:
5118 1806 1156 264
ApoI r'AATT_y
Cuts at: 1493 3428 5653 5879 6031 8140 1493
Size: 1935 2225 226 152 2109 1697
Fragments arranged by size:
2225 2109 1935 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6898 160
Size: 872 5866 1606
Fragments arranged by size:
5866 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8344
AvaI C'yCGr_G
Cuts at: 1870 1943 2556 3361 3582 3864 5514 5669 1870
Size: 73 613 805 221 282 1650 155 4545
Fragments arranged by size:
4545 1650 805 613 282 221 155 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3752 3785 3752
Size: 33 8311
BamHI G'GATC_C
Cuts at: 5674 6041 5674
Size: 367 7977
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3198 3457 4485 5409 5665
Size: 325 217 627 1411 259 1028 924 256
Cuts at: 5665 7120 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1411 1028 924 627 325 259
256 217
BanII G_rGCy'C
Cuts at: 1391 2043 2252 2563 2953 3099 3583 3925 3960
Size: 652 209 311 390 146 484 342 35
Cuts at: 3960 3984 4413 4623 4725 4893 5266 5439 5502
Size: 24 429 210 102 168 373 173 63
Cuts at: 5502 5663 1391
Size: 161 4072
Fragments arranged by size:
4072 652 484 429 390 373 342 311
210 209 173 168 161 146 102 63
35 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3961 4292 4680 928
Size: 971 2062 331 388 4592
Fragments arranged by size:
4592 2062 971 388 331
BbvCI CC'TCA_GC
Cuts at: 2874 2955 4884 2874
Size: 81 1929 6334
Fragments arranged by size:
6334 1929 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6535 6569 1903
Size: 34 4598 34 3678
Fragments arranged by size:
4598 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2338 6236 7446 2338
Size: 3898 1210 3236
Fragments arranged by size:
3898 3236 1210
BclI T'GATC_A
Cuts at: 1548 2350 2457 1548
Size: 802 107 7435
Fragments arranged by size:
7435 802 107
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2486 3528 4408
Size: 107 122 71 631 605 814 1042 880
Cuts at: 4408 5075 5157 6955 136
Size: 667 82 1798 1525
Fragments arranged by size:
1798 1525 1042 880 814 667 631 605
122 107 82 71
BglII A'GATC_T
Cuts at: 8339 8339
Size: 8344
BlpI GC'TnA_GC
Cuts at: 2087 2544 5761 2087
Size: 457 3217 4670
Fragments arranged by size:
4670 3217 457
Bme1580I G_kGCm'C
Cuts at: 1790 2557 2563 3153 3583 3708 3925 3984 4335
Size: 767 6 590 430 125 217 59 351
Cuts at: 4335 4599 4623 5036 5266 6405 7561 1790
Size: 264 24 413 230 1139 1156 2573
Fragments arranged by size:
2573 1156 1139 767 590 430 413 351
264 230 217 125 59 24 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2974 3150 6075
Size: 416 53 83 399 24 1557 176 2925
Cuts at: 6075 7033 442
Size: 958 1753
Fragments arranged by size:
2925 1753 1557 958 416 399 176 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8344
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4495 4495
Size: 8344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2648 3227 3803 4322 4463 4465 4919 7004
Size: 1409 579 576 519 141 2 454 2085
Cuts at: 7004 1239
Size: 2579
Fragments arranged by size:
2579 2085 1409 579 576 519 454 141
2
Bpu10I CC'TnA_GC
Cuts at: 2189 2874 2955 3629 3825 3908 4724 4884 2189
Size: 685 81 674 196 83 816 160 5649
Fragments arranged by size:
5649 816 685 674 196 160 83 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4322 4364 6469 7334 7632 4322
Size: 42 2105 865 298 5034
Fragments arranged by size:
5034 2105 865 298 42
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3311 4483 5264 7007 882
Size: 617 1812 1172 781 1743 2219
Fragments arranged by size:
2219 1812 1743 1172 781 617
BsaAI yAC'GTr
Cuts at: 493 1204 2425 2659 4357 5641 6135 493
Size: 711 1221 234 1698 1284 494 2702
Fragments arranged by size:
2702 1698 1284 1221 711 494 234
BsaBI GATnn'nnATC
Cuts at: 2349 8338 2349
Size: 5989 2355
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 3036 3886 6151
Size: 53 83 186 936 624 879 850 2265
Cuts at: 6151 6533 7168 275
Size: 382 635 1451
Fragments arranged by size:
2265 1451 936 879 850 635 624 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3444 5238 6777 7175 7449 7596 8007 3444
Size: 1794 1539 398 274 147 411 3781
Fragments arranged by size:
3781 1794 1539 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2831 2861 2954 2984 3200
Size: 30 165 30 1428 30 93 30 216
Cuts at: 3200 3230 3920 3950 1178
Size: 30 690 30 5572
Fragments arranged by size:
5572 1428 690 216 165 93 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2717 2943 3281 4481 4484 4754 4901
Size: 865 666 226 338 1200 3 270 147
Cuts at: 4901 4988 1186
Size: 87 4542
Fragments arranged by size:
4542 1200 865 666 338 270 226 147
87 3
BseYI C'CCAG_C
Cuts at: 2204 2584 3109 3122 3173 3185 3344 3880 4123
Size: 380 525 13 51 12 159 536 243
Cuts at: 4123 4425 4791 4867 5029 5358 5536 7547 8043
Size: 302 366 76 162 329 178 2011 496
Cuts at: 8043 2204
Size: 2505
Fragments arranged by size:
2505 2011 536 525 496 380 366 329
302 243 178 162 159 76 51 13
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2702 3027 4798 4976 5006 5489 1163
Size: 573 966 325 1771 178 30 483 4018
Fragments arranged by size:
4018 1771 966 573 483 325 178 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3516 5191 5794 6555 7583 1540
Size: 275 186 1515 1675 603 761 1028 2301
Fragments arranged by size:
2301 1675 1515 1028 761 603 275 186
BsiHKAI G_wGCw'C
Cuts at: 3018 3099 3735 3960 4335 4599 4725 4893 5439
Size: 81 636 225 375 264 126 168 546
Cuts at: 5439 6405 6490 7561 3018
Size: 966 85 1071 3801
Fragments arranged by size:
3801 1071 966 636 546 375 264 225
168 126 85 81
BsmI GAATG_Cn'
Cuts at: 3830 5855 5948 3830
Size: 2025 93 6226
Fragments arranged by size:
6226 2025 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3043 3043
Size: 8344
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2252 2557 2563 2953 3018 3099
Size: 399 253 209 305 6 390 65 81
Cuts at: 3099 3153 3583 3708 3735 3925 3960 3984 4335
Size: 54 430 125 27 190 35 24 351
Cuts at: 4335 4413 4599 4623 4725 4893 5036 5266 5439
Size: 78 186 24 102 168 143 230 173
Cuts at: 5439 5502 5663 6405 6490 7561 1391
Size: 63 161 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 430 399 390 351 305
253 230 209 190 186 173 168 161
143 125 102 85 81 78 65 63
54 35 27 24 24 6
BspEI T'CCGG_A
Cuts at: 5238 8007 5238
Size: 2769 5575
BspHI T'CATG_A
Cuts at: 3802 6233 7963 3802
Size: 2431 1730 4183
Fragments arranged by size:
4183 2431 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 4407 4958 5363 5699 844
Size: 1520 2043 551 405 336 3489
Fragments arranged by size:
3489 2043 1520 551 405 336
BsrBI CCG'CTC
Cuts at: 1394 1823 2506 3250 3649 3877 4099 5259 6231
Size: 429 683 744 399 228 222 1160 972
Cuts at: 6231 1394
Size: 3507
Fragments arranged by size:
3507 1160 972 744 683 429 399 228
222
BsrDI GCAATG_nn'
Cuts at: 65 1248 6839 7013 65
Size: 1183 5591 174 1396
Fragments arranged by size:
5591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2769 3554 3597 4799 5280 6988 7175
Size: 110 959 785 43 1202 481 1708 187
Cuts at: 7175 1700
Size: 2869
Fragments arranged by size:
2869 1708 1202 959 785 481 187 110
43
BsrGI T'GTAC_A
Cuts at: 96 3747 4083 96
Size: 3651 336 4357
Fragments arranged by size:
4357 3651 336
BssHII G'CGCG_C
Cuts at: 1925 4322 1925
Size: 2397 5947
BssSI C'ACGA_G
Cuts at: 6404 7416 6404
Size: 1012 7332
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2527 3418 794
Size: 1733 891 5720
Fragments arranged by size:
5720 1733 891
BstBI TT'CG_AA
Cuts at: 2007 7148 2007
Size: 5141 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8344
BstXI CCAn_nnnn'nTGG
Cuts at: 3310 3388 4640 3310
Size: 78 1252 7014
Fragments arranged by size:
7014 1252 78
BstYI r'GATC_y
Cuts at: 1972 2309 3853 4902 5674 6041 6426 6443 7895
Size: 337 1544 1049 772 367 385 17 1452
Cuts at: 7895 7981 7993 8339 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1544 1452 1049 772 385 367 346
337 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3372 5173 5526 3372
Size: 1801 353 6190
Fragments arranged by size:
6190 1801 353
BtgI C'CryG_G
Cuts at: 513 1065 2060 2229 2538 2664 3019 3721 5070
Size: 552 995 169 309 126 355 702 1349
Cuts at: 5070 5252 5607 513
Size: 182 355 3250
Fragments arranged by size:
3250 1349 995 702 552 355 355 309
182 169 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2854 487
Size: 683 56 1628 5977
Fragments arranged by size:
5977 1628 683 56
BtsI GCAGTG_nn'
Cuts at: 2849 3660 3708 4241 4746 4776 5411 5526 5860
Size: 811 48 533 505 30 635 115 334
Cuts at: 5860 6654 6674 2849
Size: 794 20 4519
Fragments arranged by size:
4519 811 794 635 533 505 334 115
48 30 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8344
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8309
DraI TTT'AAA
Cuts at: 5648 5648
Size: 8344
DraIII CAC_nnn'GTG
Cuts at: 4236 4693 5050 4236
Size: 457 357 7530
Fragments arranged by size:
7530 457 357
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7351 809
Size: 507 6035 1802
Fragments arranged by size:
6035 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2661 2898
Size: 54 1425 50 275 18 246 585 237
Cuts at: 2898 4660 5094 5791 6680 8309 8
Size: 1762 434 697 889 1629 43
Fragments arranged by size:
1762 1629 1425 889 697 585 434 275
246 237 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5791 1537
Size: 275 3979 4090
Fragments arranged by size:
4090 3979 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 3104 3582 3984 4418 4634 4713 4824
Size: 774 977 478 402 434 216 79 111
Cuts at: 4824 5628 6274 1353
Size: 804 646 3423
Fragments arranged by size:
3423 977 804 774 646 478 434 402
216 111 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2184 6931 7305 7451 387
Size: 1064 138 595 4747 374 146 1280
Fragments arranged by size:
4747 1280 1064 595 374 146 138
EcoICRI GAG'CTC
Cuts at: 3097 3958 4723 4891 5437 3097
Size: 861 765 168 546 6004
Fragments arranged by size:
6004 861 765 546 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2648 2791 3077 3227 3803 4322 4463
Size: 393 1016 143 286 150 576 519 141
Cuts at: 4463 4465 4919 6407 7004 7791 8059 1239
Size: 2 454 1488 597 787 268 1524
Fragments arranged by size:
1524 1488 1016 787 597 576 519 454
393 286 268 150 143 141 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2531 3848 4313 4340 2531
Size: 1317 465 27 6535
Fragments arranged by size:
6535 1317 465 27
EcoO109I rG'GnC_Cy
Cuts at: 2524 2878 3163 3280 3367 3376 3625 3980 3981
Size: 354 285 117 87 9 249 355 1
Cuts at: 3981 4537 4788 4864 5262 5263 2524
Size: 556 251 76 398 1 5605
Fragments arranged by size:
5605 556 398 355 354 285 251 249
117 87 76 9 1 1
EcoRI G'AATT_C
Cuts at: 3428 5653 6031 3428
Size: 2225 378 5741
Fragments arranged by size:
5741 2225 378
EcoRV GAT'ATC
Cuts at: 4195 4195
Size: 8344
FspI TGC'GCA
Cuts at: 5630 6850 5630
Size: 1220 7124
HaeII r_GCGC'y
Cuts at: 1678 2398 2577 3004 3033 3123 3329 3604 4379
Size: 720 179 427 29 90 206 275 775
Cuts at: 4379 4387 4432 4987 7491 1678
Size: 8 45 555 2504 2531
Fragments arranged by size:
2531 2504 775 720 555 427 275 206
179 90 45 29 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2301 2333 2372
Size: 32 135 32 518 32 179 32 39
Cuts at: 2372 2404 2867 2899 2954 2986 4151 4183 4310
Size: 32 463 32 55 32 1165 32 127
Cuts at: 4310 4342 4355 4387 4894 4926 5603 5635 6985
Size: 32 13 32 507 32 677 32 1350
Cuts at: 6985 7017 7059 7091 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1350 1165 677 518 507 463 179
135 127 55 42 39 32 32 32
32 32 32 32 32 32 32 32
32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4519 4963 5688 5934 669
Size: 988 281 2581 444 725 246 3079
Fragments arranged by size:
3079 2581 988 725 444 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8344
HpaI GTT'AAC
Cuts at: 5934 5934
Size: 8344
KpnI G_GTAC'C
Cuts at: 4489 5413 5669 4489
Size: 924 256 7164
Fragments arranged by size:
7164 924 256
MfeI C'AATT_G
Cuts at: 4608 5943 4608
Size: 1335 7009
MluI A'CGCG_T
Cuts at: 5506 8220 5506
Size: 2714 5630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2829 4149 7458 7642 1305
Size: 1524 1320 3309 184 2007
Fragments arranged by size:
3309 2007 1524 1320 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2078 5096 10
Size: 54 1425 343 246 3018 3258
Fragments arranged by size:
3258 3018 1425 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2148 2738 2744 3455 4359 4800 5372
Size: 648 982 590 6 711 904 441 572
Cuts at: 5372 5429 6302 6661 6820 518
Size: 57 873 359 159 2042
Fragments arranged by size:
2042 982 904 873 711 648 590 572
441 359 159 57 6
MspA1I CmG'CkG
Cuts at: 1606 3109 3919 3997 4215 4294 4735 5214 5609
Size: 1503 810 78 218 79 441 479 395
Cuts at: 5609 5760 6437 7585 7830 1606
Size: 151 677 1148 245 2120
Fragments arranged by size:
2120 1503 1148 810 677 479 441 395
245 218 151 79 78
NaeI GCC'GGC
Cuts at: 3556 3599 3556
Size: 43 8301
NcoI C'CATG_G
Cuts at: 513 1065 2229 2538 3721 5070 513
Size: 552 1164 309 1183 1349 3787
Fragments arranged by size:
3787 1349 1183 1164 552 309
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8344
NgoMIV G'CCGG_C
Cuts at: 3554 3597 3554
Size: 43 8301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8344
NotI GC'GGCC_GC
Cuts at: 5791 5791
Size: 8344
NspI r_CATG'y
Cuts at: 1132 1229 4799 5702 7247 1132
Size: 97 3570 903 1545 2229
Fragments arranged by size:
3570 2229 1545 903 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8344
PciI A'CATG_T
Cuts at: 7243 7243
Size: 8344
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4054 4693 5050 5071 5358 1108
Size: 2946 639 357 21 287 4094
Fragments arranged by size:
4094 2946 639 357 287 21
PfoI T'CCnGG_A
Cuts at: 3972 4905 5439 3972
Size: 933 534 6877
Fragments arranged by size:
6877 933 534
PmeI GTTT'AAAC
Cuts at: 5648 5648
Size: 8344
PmlI CAC'GTG
Cuts at: 2425 4357 2425
Size: 1932 6412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 6413 6445 7926 7958 3920
Size: 32 2461 32 1481 32 4306
Fragments arranged by size:
4306 2461 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3367 3625 4788 4864 3367
Size: 258 1163 76 6847
Fragments arranged by size:
6847 1163 258 76
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8344
PsiI TTA'TAA
Cuts at: 5914 5914
Size: 8344
PspOMI G'GGCC_C
Cuts at: 2559 3579 3921 3980 4619 5262 2559
Size: 1020 342 59 639 643 5641
Fragments arranged by size:
5641 1020 643 639 342 59
PstI C_TGCA'G
Cuts at: 830 2532 2920 3171 3295 3423 4017 5696 830
Size: 1702 388 251 124 128 594 1679 3478
Fragments arranged by size:
3478 1702 1679 594 388 251 128 124
PvuI CG_AT'CG
Cuts at: 2001 5191 2001
Size: 3190 5154
PvuII CAG'CTG
Cuts at: 3109 5214 3109
Size: 2105 6239
SacI G_AGCT'C
Cuts at: 3099 3960 4725 4893 5439 3099
Size: 861 765 168 546 6004
Fragments arranged by size:
6004 861 765 546 168
SacII CC_GC'GG
Cuts at: 5610 5610
Size: 8344
SalI G'TCGA_C
Cuts at: 1936 5686 1936
Size: 3750 4594
SanDI GG'GwC_CC
Cuts at: 3367 4788 3367
Size: 1421 6923
SapI GCTCTTCn'nnn_
Cuts at: 3104 3984 4418 4634 4713 3104
Size: 880 434 216 79 6735
Fragments arranged by size:
6735 880 434 216 79
SbfI CC_TGCA'GG
Cuts at: 5696 5696
Size: 8344
ScaI AGT'ACT
Cuts at: 6592 8304 6592
Size: 1712 6632
SexAI A'CCwGG_T
Cuts at: 4047 5046 4047
Size: 999 7345
SfcI C'TryA_G
Cuts at: 826 1045 2528 2916 3167 3291 3419 3566 3914
Size: 219 1483 388 251 124 128 147 348
Cuts at: 3914 4013 5692 6827 7508 7699 826
Size: 99 1679 1135 681 191 1471
Fragments arranged by size:
1679 1483 1471 1135 681 388 348 251
219 191 147 128 124 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 5075 5075
Size: 8344
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8344
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8344
SmaI CCC'GGG
Cuts at: 1872 2558 3584 3866 5516 5671 1872
Size: 686 1026 282 1650 155 4545
Fragments arranged by size:
4545 1650 1026 686 282 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4337 4379 6448 7349 7611 820
Size: 197 926 2394 42 2069 901 262 1553
Fragments arranged by size:
2394 2069 1553 926 901 262 197 42
SnaBI TAC'GTA
Cuts at: 493 5641 6135 493
Size: 5148 494 2702
Fragments arranged by size:
5148 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8344
SphI G_CATG'C
Cuts at: 1132 4799 5702 1132
Size: 3667 903 3774
Fragments arranged by size:
3774 3667 903
SrfI GCCC'GGGC
Cuts at: 2558 2558
Size: 8344
SspI AAT'ATT
Cuts at: 5 52 6268 5
Size: 47 6216 2081
Fragments arranged by size:
6216 2081 47
StuI AGG'CCT
Cuts at: 1517 4448 1517
Size: 2931 5413
StyI C'CwwG_G
Cuts at: 513 1065 2119 2229 2538 3042 3613 3721 5070
Size: 552 1054 110 309 504 571 108 1349
Cuts at: 5070 5783 513
Size: 713 3074
Fragments arranged by size:
3074 1349 1054 713 571 552 504 309
110 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2323 3734 5365 6371 6388 6541 6726
Size: 1255 743 1411 1631 1006 17 153 185
Cuts at: 6726 7171 325
Size: 445 1498
Fragments arranged by size:
1631 1498 1411 1255 1006 743 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3747 4083 4554
Size: 274 80 33 51 1443 1770 336 471
Cuts at: 4554 6590 8302 96
Size: 2036 1712 138
Fragments arranged by size:
2036 1770 1712 1443 471 336 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8304 8336 8304
Size: 32 8312
Tth111I GACn'n_nGTC
Cuts at: 1316 3623 1316
Size: 2307 6037
XbaI T'CTAG_A
Cuts at: 2698 5680 2698
Size: 2982 5362
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2717 3251 3307 5355 2717
Size: 534 56 2048 5706
Fragments arranged by size:
5706 2048 534 56
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8344
XmaI C'CCGG_G
Cuts at: 1870 2556 3582 3864 5514 5669 1870
Size: 686 1026 282 1650 155 4545
Fragments arranged by size:
4545 1650 1026 686 282 155
XmnI GAAnn'nnTTC
Cuts at: 3131 3432 6473 3131
Size: 301 3041 5002
Fragments arranged by size:
5002 3041 301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6152 276
Size: 53 83 186 936 4618 2468
Fragments arranged by size:
4618 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BbeI BfrBI BsiWI BstZ17I FalI FseI
FspAI KasI NarI NruI NsiI PacI PspXI PsrI
RsrII SfoI SwaI