Restriction map for
pFN21ASDA0781
(Circular) (Six-base) MAPSORT of: pFN21ASDA0781.seq Check: 7756 from: 1 to: 7507
pFN21ASDA0781 7507 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5317 278
Size: 53 83 186 936 3781 2468
Fragments arranged by size:
3781 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4850 1937
Size: 2913 4594
Acc65I G'GTAC_C
Cuts at: 4828 4828
Size: 7507
AclI AA'CG_TT
Cuts at: 1563 5634 6007 1563
Size: 4071 373 3063
Fragments arranged by size:
4071 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2394 3745 3812 4178 4403 5570 6954 7222
Size: 762 1351 67 366 225 1167 1384 268
Cuts at: 7222 1632
Size: 1917
Fragments arranged by size:
1917 1384 1351 1167 762 366 268 225
67
AfeI AGC'GCT
Cuts at: 4582 4582
Size: 7507
AflII C'TTAA_G
Cuts at: 820 1017 3626 820
Size: 197 2609 4701
Fragments arranged by size:
4701 2609 197
AflIII A'CryG_T
Cuts at: 1203 6406 7383 1203
Size: 5203 977 1327
Fragments arranged by size:
5203 1327 977
AgeI A'CCGG_T
Cuts at: 6338 6338
Size: 7507
AhdI GACnn_n'nnGTC
Cuts at: 6236 6236
Size: 7507
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7507
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3132 1184
Size: 1948 5559
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4155 4187 4155
Size: 32 7475
AlwNI CAG_nnn'CTG
Cuts at: 2923 3233 3349 3715 3835 4045 4060 4156 4273
Size: 310 116 366 120 210 15 96 117
Cuts at: 4273 4342 4426 4532 5250 6822 2923
Size: 69 84 106 718 1572 3608
Fragments arranged by size:
3608 1572 718 366 310 210 120 117
116 106 96 84 69 15
ApaLI G'TGCA_C
Cuts at: 2438 5564 6720 2438
Size: 3126 1156 3225
Fragments arranged by size:
3225 3126 1156
ApoI r'AATT_y
Cuts at: 1493 2331 2387 2515 3522 3852 4816 5042 5194
Size: 838 56 128 1007 330 964 226 152
Cuts at: 5194 7303 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1007 964 838 330 226 152
128 56
AseI AT'TA_AT
Cuts at: 160 1032 6061 160
Size: 872 5029 1606
Fragments arranged by size:
5029 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7507
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 4446 4832 1870
Size: 73 98 2405 386 4545
Fragments arranged by size:
4545 2405 386 98 73
BamHI G'GATC_C
Cuts at: 4768 4837 5204 4768
Size: 69 367 7071
Fragments arranged by size:
7071 367 69
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2136 3919 4828 6283 618
Size: 325 217 627 349 1783 909 1455 1842
Fragments arranged by size:
1842 1783 1455 909 627 349 325 217
BanII G_rGCy'C
Cuts at: 1391 2665 3141 3722 4250 4349 4639 4826 1391
Size: 1274 476 581 528 99 290 187 4072
Fragments arranged by size:
4072 1274 581 528 476 290 187 99
BbeI G_GCGC'C
Cuts at: 3923 3923
Size: 7507
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3420 3618 928
Size: 971 1521 198 4817
Fragments arranged by size:
4817 1521 971 198
BbvCI CC'TCA_GC
Cuts at: 3933 4394 3933
Size: 461 7046
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2032 2066 5698 5732 1903
Size: 34 95 34 3632 34 3678
Fragments arranged by size:
3678 3632 95 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4632 5399 6609 4632
Size: 767 1210 5530
Fragments arranged by size:
5530 1210 767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7507
BfrBI ATG'CAT
Cuts at: 3154 3154
Size: 7507
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3434 3710 6118
Size: 107 122 71 631 605 1762 276 2408
Cuts at: 6118 136
Size: 1525
Fragments arranged by size:
2408 1762 1525 631 605 276 122 107
71
BglII A'GATC_T
Cuts at: 7502 7502
Size: 7507
BlpI GC'TnA_GC
Cuts at: 3105 4924 3105
Size: 1819 5688
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2442 5568 6724 1790
Size: 306 346 3126 1156 2573
Fragments arranged by size:
3126 2573 1156 346 306
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7507
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3050 3137 4048
Size: 416 53 83 399 24 1633 87 911
Cuts at: 4048 5238 6196 442
Size: 1190 958 1753
Fragments arranged by size:
1753 1633 1190 958 911 416 399 87
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3954 1056
Size: 2898 4609
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4381 4714 4381
Size: 333 7174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2644 3410 3735 3923 4058 4067 4157 4376
Size: 1405 766 325 188 135 9 90 219
Cuts at: 4376 4684 6167 1239
Size: 308 1483 2579
Fragments arranged by size:
2579 1483 1405 766 325 308 219 188
135 90 9
Bpu10I CC'TnA_GC
Cuts at: 3933 4343 4394 3933
Size: 410 51 7046
Fragments arranged by size:
7046 410 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2225 4166 5632 6497 6795 2225
Size: 1941 1466 865 298 2937
Fragments arranged by size:
2937 1941 1466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2276 2851 3362 3682 3753 4043 6170
Size: 617 777 575 511 320 71 290 2127
Cuts at: 6170 882
Size: 2219
Fragments arranged by size:
2219 2127 777 617 575 511 320 290
71
BsaAI yAC'GTr
Cuts at: 493 1204 4804 5298 493
Size: 711 3600 494 2702
Fragments arranged by size:
3600 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7501 7501
Size: 7507
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3920 5314 5696 6331
Size: 53 83 186 936 2387 1394 382 635
Cuts at: 6331 275
Size: 1451
Fragments arranged by size:
2387 1451 1394 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2139 2724 3898 4439 5940 6338 6612 6759 7170
Size: 585 1174 541 1501 398 274 147 411
Cuts at: 7170 2139
Size: 2476
Fragments arranged by size:
2476 1501 1174 585 541 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3530 3560 4040 4070 4155
Size: 30 165 30 2127 30 480 30 85
Cuts at: 4155 4185 1178
Size: 30 4500
Fragments arranged by size:
4500 2127 480 165 85 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3179 3921 4446 4449 1186
Size: 1993 742 525 3 4244
Fragments arranged by size:
4244 1993 742 525 3
BseYI C'CCAG_C
Cuts at: 2098 2128 3353 3700 4023 4082 4335 4537 4638
Size: 30 1225 347 323 59 253 202 101
Cuts at: 4638 6710 7206 2098
Size: 2072 496 2399
Fragments arranged by size:
2399 2072 1225 496 347 323 253 202
101 59 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3893 1163
Size: 573 2157 4777
Fragments arranged by size:
4777 2157 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4957 5718 6746 1540
Size: 275 186 2956 761 1028 2301
Fragments arranged by size:
2956 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2442 2665 2760 3722 4250 5568 5653 6724 2442
Size: 223 95 962 528 1318 85 1071 3225
Fragments arranged by size:
3225 1318 1071 962 528 223 95 85
BsmI GAATG_Cn'
Cuts at: 3154 3254 5018 5111 3154
Size: 100 1764 93 5550
Fragments arranged by size:
5550 1764 100 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3457 4000 3457
Size: 543 6964
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2442 2665 2760 3141 3722 4250
Size: 399 306 346 223 95 381 581 528
Cuts at: 4250 4349 4639 4826 5568 5653 6724 1391
Size: 99 290 187 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 581 528 399 381 346
306 290 223 187 99 95 85
BspEI T'CCGG_A
Cuts at: 2724 7170 2724
Size: 4446 3061
BspHI T'CATG_A
Cuts at: 5396 7126 5396
Size: 1730 5777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2908 4068 4862 844
Size: 2064 1160 794 3489
Fragments arranged by size:
3489 2064 1160 794
BsrBI CCG'CTC
Cuts at: 1394 1823 3422 5394 1394
Size: 429 1599 1972 3507
Fragments arranged by size:
3507 1972 1599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3099 6002 6176 65
Size: 1183 1851 2903 174 1396
Fragments arranged by size:
2903 1851 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 2358 3922 6151 6338 1700
Size: 110 252 296 1564 2229 187 2869
Fragments arranged by size:
2869 2229 1564 296 252 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7507
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7507
BssSI C'ACGA_G
Cuts at: 5567 6579 5567
Size: 1012 6495
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2923 4156 794
Size: 2129 1233 4145
Fragments arranged by size:
4145 2129 1233
BstBI TT'CG_AA
Cuts at: 2007 6311 2007
Size: 4304 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7507
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 3496 2029
Size: 1467 6040
BstYI r'GATC_y
Cuts at: 1972 4768 4837 5204 5589 5606 7058 7144 7156
Size: 2796 69 367 385 17 1452 86 12
Cuts at: 7156 7502 1972
Size: 346 1977
Fragments arranged by size:
2796 1977 1452 385 367 346 86 69
17 12
Bsu36I CC'TnA_GG
Cuts at: 3963 3963
Size: 7507
BtgI C'CryG_G
Cuts at: 513 1065 2022 4034 4136 513
Size: 552 957 2012 102 3884
Fragments arranged by size:
3884 2012 957 552 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6768
Fragments arranged by size:
6768 683 56
BtsI GCAGTG_nn'
Cuts at: 2202 3347 5023 5817 5837 2202
Size: 1145 1676 794 20 3872
Fragments arranged by size:
3872 1676 1145 794 20
ClaI AT'CG_AT
Cuts at: 1554 2175 1554
Size: 621 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7472
DraI TTT'AAA
Cuts at: 2524 4811 2524
Size: 2287 5220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6514 809
Size: 507 5198 1802
Fragments arranged by size:
5198 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2356 3076 4954
Size: 54 1425 50 275 18 526 720 1878
Cuts at: 4954 5843 7472 8
Size: 889 1629 43
Fragments arranged by size:
1878 1629 1425 889 720 526 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4954 1537
Size: 275 3142 4090
Fragments arranged by size:
4090 3142 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2691 3261 5437 1353
Size: 1338 570 2176 3423
Fragments arranged by size:
3423 2176 1338 570
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2048 3260 6094 6468 6614 387
Size: 1064 138 459 1212 2834 374 146 1280
Fragments arranged by size:
2834 1280 1212 1064 459 374 146 138
EcoICRI GAG'CTC
Cuts at: 2663 3720 4248 2663
Size: 1057 528 5922
Fragments arranged by size:
5922 1057 528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2394 2644 3410 3735 3745 3812 3923
Size: 393 762 250 766 325 10 67 111
Cuts at: 3923 4058 4067 4157 4178 4376 4403 4684 5570
Size: 135 9 90 21 198 27 281 886
Cuts at: 5570 6167 6954 7222 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 886 787 766 762 597 393 325
281 268 250 198 135 111 90 67
27 21 10 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3194 3513 3797 3194
Size: 319 284 6904
Fragments arranged by size:
6904 319 284
EcoO109I rG'GnC_Cy
Cuts at: 3595 3943 4315 4693 3595
Size: 348 372 378 6409
Fragments arranged by size:
6409 378 372 348
EcoRI G'AATT_C
Cuts at: 3852 4816 5194 3852
Size: 964 378 6165
Fragments arranged by size:
6165 964 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2128 4204 4602 2128
Size: 2076 398 5033
Fragments arranged by size:
5033 2076 398
FspI TGC'GCA
Cuts at: 6013 6013
Size: 7507
HaeII r_GCGC'y
Cuts at: 1678 3031 3923 4584 6654 1678
Size: 1353 892 661 2070 2531
Fragments arranged by size:
2531 2070 1353 892 661
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2850 2882 3171 3203 3530
Size: 32 135 32 1278 32 289 32 327
Cuts at: 3530 3562 3566 3598 6148 6180 6222 6254 1373
Size: 32 4 32 2550 32 42 32 2626
Fragments arranged by size:
2626 2550 1278 327 289 135 42 32
32 32 32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4851 5097 669
Size: 988 281 2913 246 3079
Fragments arranged by size:
3079 2913 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7507
HpaI GTT'AAC
Cuts at: 5097 5097
Size: 7507
KasI G'GCGC_C
Cuts at: 3919 3919
Size: 7507
KpnI G_GTAC'C
Cuts at: 4832 4832
Size: 7507
MfeI C'AATT_G
Cuts at: 5106 5106
Size: 7507
MluI A'CGCG_T
Cuts at: 7383 7383
Size: 7507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2408 3228 6621 6805 1305
Size: 1103 820 3393 184 2007
Fragments arranged by size:
3393 2007 1103 820 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5685
Fragments arranged by size:
5685 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 5465 5824 5983 518
Size: 648 861 3438 359 159 2042
Fragments arranged by size:
3438 2042 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2188 2614 3456 3957 4027 4138 4156 4273
Size: 582 426 842 501 70 111 18 117
Cuts at: 4273 4923 5600 6748 6993 1606
Size: 650 677 1148 245 2120
Fragments arranged by size:
2120 1148 842 677 650 582 501 426
245 117 111 70 18
NaeI GCC'GGC
Cuts at: 3924 3924
Size: 7507
NarI GG'CG_CC
Cuts at: 3920 3920
Size: 7507
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5998
Fragments arranged by size:
5998 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7507
NgoMIV G'CCGG_C
Cuts at: 3922 3922
Size: 7507
NheI G'CTAG_C
Cuts at: 1052 3950 1052
Size: 2898 4609
NotI GC'GGCC_GC
Cuts at: 4954 4954
Size: 7507
NsiI A_TGCA'T
Cuts at: 3156 3156
Size: 7507
NspI r_CATG'y
Cuts at: 1132 1229 4865 6410 1132
Size: 97 3636 1545 2229
Fragments arranged by size:
3636 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3799 1348
Size: 2451 5056
PciI A'CATG_T
Cuts at: 6406 6406
Size: 7507
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3233 1108
Size: 2125 5382
PfoI T'CCnGG_A
Cuts at: 3939 4353 3939
Size: 414 7093
PmeI GTTT'AAAC
Cuts at: 4811 4811
Size: 7507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4155 4187 5576 5608 7089 7121 4155
Size: 32 1389 32 1481 32 4541
Fragments arranged by size:
4541 1481 1389 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3595 3943 3595
Size: 348 7159
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7507
PsiI TTA'TAA
Cuts at: 5077 5077
Size: 7507
PstI C_TGCA'G
Cuts at: 830 4065 4161 4215 4859 830
Size: 3235 96 54 644 3478
Fragments arranged by size:
3478 3235 644 96 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7507
PvuII CAG'CTG
Cuts at: 2188 2614 3957 4027 4156 4273 2188
Size: 426 1343 70 129 117 5422
Fragments arranged by size:
5422 1343 426 129 117 70
RsrII CG'GwC_CG
Cuts at: 2065 2065
Size: 7507
SacI G_AGCT'C
Cuts at: 2665 3722 4250 2665
Size: 1057 528 5922
Fragments arranged by size:
5922 1057 528
SacII CC_GC'GG
Cuts at: 4139 4139
Size: 7507
SalI G'TCGA_C
Cuts at: 1936 4849 1936
Size: 2913 4594
SbfI CC_TGCA'GG
Cuts at: 4859 4859
Size: 7507
ScaI AGT'ACT
Cuts at: 5755 7467 5755
Size: 1712 5795
SfcI C'TryA_G
Cuts at: 826 1045 4061 4157 4211 4475 4855 5990 6671
Size: 219 3016 96 54 264 380 1135 681
Cuts at: 6671 6862 826
Size: 191 1471
Fragments arranged by size:
3016 1471 1135 681 380 264 219 191
96 54
SfoI GGC'GCC
Cuts at: 3921 3921
Size: 7507
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7507
SgrAI Cr'CCGG_yG
Cuts at: 1700 3922 1700
Size: 2222 5285
SmaI CCC'GGG
Cuts at: 1872 4448 4834 1872
Size: 2576 386 4545
Fragments arranged by size:
4545 2576 386
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2204 3626 4145 5611 6512 6774
Size: 197 926 261 1422 519 1466 901 262
Cuts at: 6774 820
Size: 1553
Fragments arranged by size:
1553 1466 1422 926 901 519 262 261
197
SnaBI TAC'GTA
Cuts at: 493 4804 5298 493
Size: 4311 494 2702
Fragments arranged by size:
4311 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7507
SphI G_CATG'C
Cuts at: 1132 4865 1132
Size: 3733 3774
SspI AAT'ATT
Cuts at: 5 52 5431 5
Size: 47 5379 2081
Fragments arranged by size:
5379 2081 47
StuI AGG'CCT
Cuts at: 1517 3215 4217 1517
Size: 1698 1002 4807
Fragments arranged by size:
4807 1698 1002
StyI C'CwwG_G
Cuts at: 513 1065 2022 2941 3120 4946 513
Size: 552 957 919 179 1826 3074
Fragments arranged by size:
3074 1826 957 919 552 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2695 3812 5534 5551 5704 5889 6334
Size: 1255 1115 1117 1722 17 153 185 445
Cuts at: 6334 325
Size: 1498
Fragments arranged by size:
1722 1498 1255 1117 1115 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2224 5753 7465
Size: 274 80 33 51 1443 247 3529 1712
Cuts at: 7465 96
Size: 138
Fragments arranged by size:
3529 1712 1443 274 247 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3254 3286 4426 4458 7467 7499 3254
Size: 32 1140 32 3009 32 3262
Fragments arranged by size:
3262 3009 1140 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7507
XbaI T'CTAG_A
Cuts at: 3856 4843 3856
Size: 987 6520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2155 3361 2155
Size: 1206 6301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7507
XmaI C'CCGG_G
Cuts at: 1870 4446 4832 1870
Size: 2576 386 4545
Fragments arranged by size:
4545 2576 386
XmnI GAAnn'nnTTC
Cuts at: 3524 5636 3524
Size: 2112 5395
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5315 276
Size: 53 83 186 936 3781 2468
Fragments arranged by size:
3781 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BsiWI BstZ17I DraIII
EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI
PsrI SanDI SapI SexAI SfiI SrfI SwaI