Restriction map for
pFN21ASDA0778
(Circular) (Six-base) MAPSORT of: pFN21ASDA0778.seq Check: 3317 from: 1 to: 7789
pFN21ASDA0778 7789 bp
With 182 enzymes: SgfI *
September 25, 2018 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2062 2062
Size: 7789
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4236 5599 278
Size: 53 83 186 936 2700 1363 2468
Fragments arranged by size:
2700 2468 1363 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5132 1937
Size: 3195 4594
Acc65I G'GTAC_C
Cuts at: 2509 5110 2509
Size: 2601 5188
AclI AA'CG_TT
Cuts at: 1563 5916 6289 1563
Size: 4353 373 3063
Fragments arranged by size:
4353 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2141 4043 5852 7236 7504 1632
Size: 509 1902 1809 1384 268 1917
Fragments arranged by size:
1917 1902 1809 1384 509 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7592
AflIII A'CryG_T
Cuts at: 1203 3186 6688 7665 1203
Size: 1983 3502 977 1327
Fragments arranged by size:
3502 1983 1327 977
AgeI A'CCGG_T
Cuts at: 3561 6620 3561
Size: 3059 4730
AhdI GACnn_n'nnGTC
Cuts at: 6518 6518
Size: 7789
AleI CACnn'nnGTG
Cuts at: 1166 4295 1166
Size: 3129 4660
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7789
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2874 2906 2874
Size: 32 7757
AlwNI CAG_nnn'CTG
Cuts at: 3818 4600 5532 7104 3818
Size: 782 932 1572 4503
Fragments arranged by size:
4503 1572 932 782
ApaI G_GGCC'C
Cuts at: 2247 3544 2247
Size: 1297 6492
ApaLI G'TGCA_C
Cuts at: 4007 5846 7002 4007
Size: 1839 1156 4794
Fragments arranged by size:
4794 1839 1156
ApoI r'AATT_y
Cuts at: 1493 3722 5098 5324 5476 7585 1493
Size: 2229 1376 226 152 2109 1697
Fragments arranged by size:
2229 2109 1697 1376 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6343 160
Size: 872 5311 1606
Fragments arranged by size:
5311 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7789
AvaI C'yCGr_G
Cuts at: 1870 1943 2235 2702 3711 3798 4367 5114 1870
Size: 73 292 467 1009 87 569 747 4545
Fragments arranged by size:
4545 1009 747 569 467 292 87 73
BamHI G'GATC_C
Cuts at: 5119 5486 5119
Size: 367 7422
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2509 3152 5110 6565 618
Size: 325 217 627 722 643 1958 1455 1842
Fragments arranged by size:
1958 1842 1455 722 643 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2221 2247 2480 2688 3122 3402 3544 3777
Size: 830 26 233 208 434 280 142 233
Cuts at: 3777 4887 5108 1391
Size: 1110 221 4072
Fragments arranged by size:
4072 1110 830 434 280 233 233 221
208 142 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3304 3510 4837 928
Size: 971 1405 206 1327 3880
Fragments arranged by size:
3880 1405 1327 971 206
BbvCI CC'TCA_GC
Cuts at: 2514 2514
Size: 7789
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3160 3194 4163 4197 5980 6014 1903
Size: 34 1223 34 969 34 1783 34 3678
Fragments arranged by size:
3678 1783 1223 969 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5058 5681 6891 5058
Size: 623 1210 5956
Fragments arranged by size:
5956 1210 623
BclI T'GATC_A
Cuts at: 1548 2898 3852 1548
Size: 1350 954 5485
Fragments arranged by size:
5485 1350 954
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 3019 4148
Size: 107 122 71 631 605 394 953 1129
Cuts at: 4148 4487 4657 6400 136
Size: 339 170 1743 1525
Fragments arranged by size:
1743 1525 1129 953 631 605 394 339
170 122 107 71
BglII A'GATC_T
Cuts at: 7784 7784
Size: 7789
BlpI GC'TnA_GC
Cuts at: 5206 5206
Size: 7789
Bme1580I G_kGCm'C
Cuts at: 1790 2247 3155 3544 4011 4424 4454 5850 7006
Size: 457 908 389 467 413 30 1396 1156
Cuts at: 7006 1790
Size: 2573
Fragments arranged by size:
2573 1396 1156 908 467 457 413 389
30
BmgBI CAC'GTC
Cuts at: 1318 3126 1318
Size: 1808 5981
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4610 4673 4690
Size: 416 53 83 399 24 3193 63 17
Cuts at: 4690 5520 6478 442
Size: 830 958 1753
Fragments arranged by size:
3193 1753 958 830 416 399 83 63
53 24 17
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7789
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3352 3352
Size: 7789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2747 2867 2990 3119 4727 4910 6449 1239
Size: 1508 120 123 129 1608 183 1539 2579
Fragments arranged by size:
2579 1608 1539 1508 183 129 123 120
Bpu10I CC'TnA_GC
Cuts at: 2220 2514 2220
Size: 294 7495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3371 4043 5914 6779 7077 3371
Size: 672 1871 865 298 4083
Fragments arranged by size:
4083 1871 865 672 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2574 2598 2723 2796 2932 3299 6452
Size: 617 1075 24 125 73 136 367 3153
Cuts at: 6452 882
Size: 2219
Fragments arranged by size:
3153 2219 1075 617 367 136 125 73
24
BsaAI yAC'GTr
Cuts at: 493 1204 3267 3523 4764 5086 5580 493
Size: 711 2063 256 1241 322 494 2702
Fragments arranged by size:
2702 2063 1241 711 494 322 256
BsaBI GATnn'nnATC
Cuts at: 2552 7783 2552
Size: 5231 2558
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4233 5596 5978 6613
Size: 53 83 186 936 2700 1363 382 635
Cuts at: 6613 275
Size: 1451
Fragments arranged by size:
2700 1451 1363 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2625 2805 3561 4689 6222 6620 6894 7041 7452
Size: 180 756 1128 1533 398 274 147 411
Cuts at: 7452 2625
Size: 2962
Fragments arranged by size:
2962 1533 1128 756 411 398 274 180
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2248 2278 2571 2595 2601
Size: 30 165 30 845 30 293 24 6
Cuts at: 2601 2625 3551 3581 4131 4161 4265 4295 4748
Size: 24 926 30 550 30 104 30 453
Cuts at: 4748 4778 4886 4916 1178
Size: 30 108 30 4051
Fragments arranged by size:
4051 926 845 550 453 293 165 108
104 30 30 30 30 30 30 30
30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4316 4907 4991 1186
Size: 3130 591 84 3984
Fragments arranged by size:
3984 3130 591 84
BseYI C'CCAG_C
Cuts at: 2167 3115 3646 3670 4098 5058 6992 7488 2167
Size: 948 531 24 428 960 1934 496 2468
Fragments arranged by size:
2468 1934 960 948 531 496 428 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3602 4820 1163
Size: 573 1866 1218 4132
Fragments arranged by size:
4132 1866 1218 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5239 6000 7028 1540
Size: 275 186 3238 761 1028 2301
Fragments arranged by size:
3238 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2480 2943 3402 3569 4011 5850 5935 7006 2480
Size: 463 459 167 442 1839 85 1071 3263
Fragments arranged by size:
3263 1839 1071 463 459 442 167 85
BsmI GAATG_Cn'
Cuts at: 3552 4775 5300 5393 3552
Size: 1223 525 93 5948
Fragments arranged by size:
5948 1223 525 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3104 4098 4240 4889 3104
Size: 994 142 649 6004
Fragments arranged by size:
6004 994 649 142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2221 2247 2480 2688 2943 3122 3155
Size: 399 431 26 233 208 255 179 33
Cuts at: 3155 3402 3544 3569 3777 4011 4424 4454 4887
Size: 247 142 25 208 234 413 30 433
Cuts at: 4887 5108 5850 5935 7006 1391
Size: 221 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 431 413 399 255
247 234 233 221 208 208 179 142
85 33 30 26 25
BspEI T'CCGG_A
Cuts at: 2625 7452 2625
Size: 4827 2962
BspHI T'CATG_A
Cuts at: 4506 5678 7408 4506
Size: 1172 1730 4887
Fragments arranged by size:
4887 1730 1172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2062 5144 844
Size: 1218 3082 3489
Fragments arranged by size:
3489 3082 1218
BsrBI CCG'CTC
Cuts at: 1394 1823 2697 3601 4960 5676 1394
Size: 429 874 904 1359 716 3507
Fragments arranged by size:
3507 1359 904 874 716 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2142 2877 3891 3942 4206 4395 6284
Size: 1183 894 735 1014 51 264 189 1889
Cuts at: 6284 6458 65
Size: 174 1396
Fragments arranged by size:
1889 1396 1183 1014 894 735 264 189
174 51
BsrFI r'CCGG_y
Cuts at: 1700 1810 3561 6433 6620 1700
Size: 110 1751 2872 187 2869
Fragments arranged by size:
2872 2869 1751 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7789
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7789
BssSI C'ACGA_G
Cuts at: 3494 5849 6861 3494
Size: 2355 1012 4422
Fragments arranged by size:
4422 2355 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4781 794
Size: 3987 3802
BstBI TT'CG_AA
Cuts at: 2007 6593 2007
Size: 4586 3203
BstEII G'GTnAC_C
Cuts at: 1184 3859 1184
Size: 2675 5114
BstXI CCAn_nnnn'nTGG
Cuts at: 2488 2872 3067 3577 3904 4477 4792 2488
Size: 384 195 510 327 573 315 5485
Fragments arranged by size:
5485 573 510 384 327 315 195
BstYI r'GATC_y
Cuts at: 1972 3594 4053 4869 5119 5486 5871 5888 7340
Size: 1622 459 816 250 367 385 17 1452
Cuts at: 7340 7426 7438 7784 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1622 1452 816 459 385 367 346
250 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2839 3015 2839
Size: 176 7613
BtgI C'CryG_G
Cuts at: 513 1065 2022 2029 2064 2376 3750 4212 513
Size: 552 957 7 35 312 1374 462 4090
Fragments arranged by size:
4090 1374 957 552 462 312 35 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4324 487
Size: 683 56 3098 3952
Fragments arranged by size:
3952 3098 683 56
BtsI GCAGTG_nn'
Cuts at: 2772 4808 5305 6099 6119 2772
Size: 2036 497 794 20 4442
Fragments arranged by size:
4442 2036 794 497 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7789
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7754
DraI TTT'AAA
Cuts at: 5093 5093
Size: 7789
DraIII CAC_nnn'GTG
Cuts at: 3042 4604 4974 3042
Size: 1562 370 5857
Fragments arranged by size:
5857 1562 370
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3277 6796 809
Size: 507 1961 3519 1802
Fragments arranged by size:
3519 1961 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 3003 3027
Size: 54 1425 50 275 18 208 965 24
Cuts at: 3027 4149 5236 6125 7754 8
Size: 1122 1087 889 1629 43
Fragments arranged by size:
1629 1425 1122 1087 965 889 275 208
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 5236 1537
Size: 275 3424 4090
Fragments arranged by size:
4090 3424 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2641 5719 1353
Size: 1288 3078 3423
Fragments arranged by size:
3423 3078 1288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2872 4661 6376 6750 6896 387
Size: 1064 138 1283 1789 1715 374 146 1280
Fragments arranged by size:
1789 1715 1283 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2478 3400 2478
Size: 922 6867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2747 2867 2990 3119 4043 4727
Size: 393 509 606 120 123 129 924 684
Cuts at: 4727 4910 5852 6449 7236 7504 1239
Size: 183 942 597 787 268 1524
Fragments arranged by size:
1524 942 924 787 684 606 597 509
393 268 183 129 123 120
EcoNI CCTnn'n_nnAGG
Cuts at: 2195 2588 3581 2195
Size: 393 993 6403
Fragments arranged by size:
6403 993 393
EcoO109I rG'GnC_Cy
Cuts at: 2200 3540 3541 2200
Size: 1340 1 6448
Fragments arranged by size:
6448 1340 1
EcoRI G'AATT_C
Cuts at: 5098 5476 5098
Size: 378 7411
EcoRV GAT'ATC
Cuts at: 4504 4504
Size: 7789
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 3781 2173
Size: 1608 6181
FspI TGC'GCA
Cuts at: 6295 6295
Size: 7789
HaeII r_GCGC'y
Cuts at: 1678 6936 1678
Size: 5258 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 2595 2627 2648
Size: 32 135 32 803 32 188 32 21
Cuts at: 2648 2680 3242 3274 3551 3583 3778 3810 4019
Size: 32 562 32 277 32 195 32 209
Cuts at: 4019 4051 6430 6462 6504 6536 1373
Size: 32 2379 32 42 32 2626
Fragments arranged by size:
2626 2379 803 562 277 209 195 188
135 42 32 32 32 32 32 32
32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 3683 3979 5133 5379 669
Size: 988 281 1745 296 1154 246 3079
Fragments arranged by size:
3079 1745 1154 988 296 281 246
HindIII A'AGCT_T
Cuts at: 748 3758 748
Size: 3010 4779
HpaI GTT'AAC
Cuts at: 5379 5379
Size: 7789
KpnI G_GTAC'C
Cuts at: 2513 5114 2513
Size: 2601 5188
MfeI C'AATT_G
Cuts at: 5388 5388
Size: 7789
MluI A'CGCG_T
Cuts at: 7665 7665
Size: 7789
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2418 2661 6903 7087 1305
Size: 1113 243 4242 184 2007
Fragments arranged by size:
4242 2007 1113 243 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3005 3029 10
Size: 54 1425 343 1173 24 4770
Fragments arranged by size:
4770 1425 1173 343 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2870 2999 3338 3578 3782 3848 3965
Size: 648 1704 129 339 240 204 66 117
Cuts at: 3965 4115 4211 4295 4457 4565 4793 5747 6106
Size: 150 96 84 162 108 228 954 359
Cuts at: 6106 6265 518
Size: 159 2042
Fragments arranged by size:
2042 1704 954 648 359 339 240 228
204 162 159 150 129 117 108 96
84 66
MspA1I CmG'CkG
Cuts at: 1606 2215 2429 2896 2964 3359 3484 4493 4744
Size: 609 214 467 68 395 125 1009 251
Cuts at: 4744 5205 5882 7030 7275 1606
Size: 461 677 1148 245 2120
Fragments arranged by size:
2120 1148 1009 677 609 467 461 395
251 245 214 125 68
NcoI C'CATG_G
Cuts at: 513 1065 2022 2376 4212 513
Size: 552 957 354 1836 4090
Fragments arranged by size:
4090 1836 957 552 354
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7789
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7789
NotI GC'GGCC_GC
Cuts at: 5236 5236
Size: 7789
NspI r_CATG'y
Cuts at: 1132 1229 3190 4002 4954 5147 6692 1132
Size: 97 1961 812 952 193 1545 2229
Fragments arranged by size:
2229 1961 1545 952 812 193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7789
PciI A'CATG_T
Cuts at: 3186 6688 3186
Size: 3502 4287
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3526 4600 1108
Size: 2418 1074 4297
Fragments arranged by size:
4297 2418 1074
PfoI T'CCnGG_A
Cuts at: 2532 4887 2532
Size: 2355 5434
PmeI GTTT'AAAC
Cuts at: 5093 5093
Size: 7789
PmlI CAC'GTG
Cuts at: 3523 4764 3523
Size: 1241 6548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5858 5890 7371 7403 5858
Size: 32 1481 32 6244
Fragments arranged by size:
6244 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7789
PsiI TTA'TAA
Cuts at: 5359 5359
Size: 7789
PspOMI G'GGCC_C
Cuts at: 2243 3540 2243
Size: 1297 6492
PstI C_TGCA'G
Cuts at: 830 5141 830
Size: 4311 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7789
PvuII CAG'CTG
Cuts at: 2429 2896 2964 3359 3484 4493 2429
Size: 467 68 395 125 1009 5725
Fragments arranged by size:
5725 1009 467 395 125 68
SacI G_AGCT'C
Cuts at: 2480 3402 2480
Size: 922 6867
SalI G'TCGA_C
Cuts at: 1936 5131 1936
Size: 3195 4594
SbfI CC_TGCA'GG
Cuts at: 5141 5141
Size: 7789
ScaI AGT'ACT
Cuts at: 2048 6037 7749 2048
Size: 3989 1712 2088
Fragments arranged by size:
3989 2088 1712
SexAI A'CCwGG_T
Cuts at: 2583 4970 2583
Size: 2387 5402
SfcI C'TryA_G
Cuts at: 826 1045 2959 4993 5137 6272 6953 7144 826
Size: 219 1914 2034 144 1135 681 191 1471
Fragments arranged by size:
2034 1914 1471 1135 681 219 191 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7789
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7789
SmaI CCC'GGG
Cuts at: 1872 3800 5116 1872
Size: 1928 1316 4545
Fragments arranged by size:
4545 1928 1316
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2235 3386 4058 5893 6794 7056
Size: 197 926 292 1151 672 1835 901 262
Cuts at: 7056 820
Size: 1553
Fragments arranged by size:
1835 1553 1151 926 901 672 292 262
197
SnaBI TAC'GTA
Cuts at: 493 5086 5580 493
Size: 4593 494 2702
Fragments arranged by size:
4593 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7789
SphI G_CATG'C
Cuts at: 1132 4002 5147 1132
Size: 2870 1145 3774
Fragments arranged by size:
3774 2870 1145
SspI AAT'ATT
Cuts at: 5 52 5713 5
Size: 47 5661 2081
Fragments arranged by size:
5661 2081 47
StuI AGG'CCT
Cuts at: 1517 2844 1517
Size: 1327 6462
StyI C'CwwG_G
Cuts at: 513 1065 2022 2157 2196 2376 3441 3885 3993
Size: 552 957 135 39 180 1065 444 108
Cuts at: 3993 4212 4476 5228 513
Size: 219 264 752 3074
Fragments arranged by size:
3074 1065 957 752 552 444 264 219
180 135 108 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2402 2726 2878 3176 4592 4925 5050
Size: 1255 822 324 152 298 1416 333 125
Cuts at: 5050 5816 5833 5986 6171 6616 325
Size: 766 17 153 185 445 1498
Fragments arranged by size:
1498 1416 1255 822 766 445 333 324
298 185 153 152 125 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2046 6035 7747
Size: 274 80 33 51 1443 69 3989 1712
Cuts at: 7747 96
Size: 138
Fragments arranged by size:
3989 1712 1443 274 138 80 69 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2138 2170 2248 2280 2571 2603 4131 4163 4748
Size: 32 78 32 291 32 1528 32 585
Cuts at: 4748 4780 7749 7781 2138
Size: 32 2969 32 2146
Fragments arranged by size:
2969 2146 1528 585 291 78 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2609 1316
Size: 1293 6496
XbaI T'CTAG_A
Cuts at: 5125 5125
Size: 7789
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4000 4601 4702 4792 4000
Size: 601 101 90 6997
Fragments arranged by size:
6997 601 101 90
XhoI C'TCGA_G
Cuts at: 1943 2235 1943
Size: 292 7497
XmaI C'CCGG_G
Cuts at: 1870 3798 5114 1870
Size: 1928 1316 4545
Fragments arranged by size:
4545 1928 1316
XmnI GAAnn'nnTTC
Cuts at: 4643 5918 4643
Size: 1275 6514
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4234 5597 276
Size: 53 83 186 936 2700 1363 2468
Fragments arranged by size:
2700 2468 1363 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI