Restriction map for
pFN21ASDA0775
(Circular) (Six-base) MAPSORT of: pFN21ASDA0775.seq Check: 7890 from: 1 to: 6574
pFN21ASDA0775 6574 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2505 2505
Size: 6574
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4384 278
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2235 3917 1937
Size: 298 1682 4594
Fragments arranged by size:
4594 1682 298
Acc65I G'GTAC_C
Cuts at: 3810 3895 3810
Size: 85 6489
AclI AA'CG_TT
Cuts at: 1563 4701 5074 1563
Size: 3138 373 3063
Fragments arranged by size:
3138 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2825 4637 6021 6289 1632
Size: 1193 1812 1384 268 1917
Fragments arranged by size:
1917 1812 1384 1193 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 2127 5473 6450 1203
Size: 924 3346 977 1327
Fragments arranged by size:
3346 1327 977 924
AgeI A'CCGG_T
Cuts at: 5405 5405
Size: 6574
AhdI GACnn_n'nnGTC
Cuts at: 5303 5303
Size: 6574
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3578 1184
Size: 2394 4180
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2018 2050 2018
Size: 32 6542
AlwNI CAG_nnn'CTG
Cuts at: 2527 2836 4317 5889 2527
Size: 309 1481 1572 3212
Fragments arranged by size:
3212 1572 1481 309
ApaI G_GGCC'C
Cuts at: 2482 2830 2482
Size: 348 6226
ApaLI G'TGCA_C
Cuts at: 4631 5787 4631
Size: 1156 5418
ApoI r'AATT_y
Cuts at: 1493 2899 3883 4109 4261 6370 1493
Size: 1406 984 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 984 226 152
AscI GG'CGCG_CC
Cuts at: 3541 3541
Size: 6574
AseI AT'TA_AT
Cuts at: 160 1032 5128 160
Size: 872 4096 1606
Fragments arranged by size:
4096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 2742 2823 3084 3489 3677 3899 1870
Size: 73 799 81 261 405 188 222 4545
Fragments arranged by size:
4545 799 405 261 222 188 81 73
BamHI G'GATC_C
Cuts at: 3904 4271 3904
Size: 367 6207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3226 3271 3440 3635 3810
Size: 325 217 627 1439 45 169 195 175
Cuts at: 3810 3895 5350 618
Size: 85 1455 1842
Fragments arranged by size:
1842 1455 1439 627 325 217 195 175
169 85 45
BanII G_rGCy'C
Cuts at: 1391 2482 2576 2672 2752 2830 3510 3764 3893
Size: 1091 94 96 80 78 680 254 129
Cuts at: 3893 1391
Size: 4072
Fragments arranged by size:
4072 1091 680 254 129 96 94 80
78
BbeI G_GCGC'C
Cuts at: 3444 3444
Size: 6574
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5603
BbvCI CC'TCA_GC
Cuts at: 2465 3530 2465
Size: 1065 5509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4765 4799 1903
Size: 34 2828 34 3678
Fragments arranged by size:
3678 2828 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4466 5676 4466
Size: 1210 5364
BclI T'GATC_A
Cuts at: 1548 2256 2890 1548
Size: 708 634 5232
Fragments arranged by size:
5232 708 634
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2747 3089 3470
Size: 107 122 71 631 605 1075 342 381
Cuts at: 3470 5185 136
Size: 1715 1525
Fragments arranged by size:
1715 1525 1075 631 605 381 342 122
107 71
BglII A'GATC_T
Cuts at: 2505 3864 6569 2505
Size: 1359 2705 2510
Fragments arranged by size:
2705 2510 1359
BlpI GC'TnA_GC
Cuts at: 2751 3991 2751
Size: 1240 5334
Bme1580I G_kGCm'C
Cuts at: 1790 2482 2830 3065 3104 3147 3155 3276 3609
Size: 692 348 235 39 43 8 121 333
Cuts at: 3609 3638 4635 5791 1790
Size: 29 997 1156 2573
Fragments arranged by size:
2573 1156 997 692 348 333 235 121
43 39 29 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6574
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2673 3052 4305
Size: 416 53 83 399 24 1256 379 1253
Cuts at: 4305 5263 442
Size: 958 1753
Fragments arranged by size:
1753 1256 1253 958 416 399 379 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2339 2531 2714 2756 3872 5234 1239
Size: 977 123 192 183 42 1116 1362 2579
Fragments arranged by size:
2579 1362 1116 977 192 183 123 42
Bpu10I CC'TnA_GC
Cuts at: 2465 2570 3530 2465
Size: 105 960 5509
Fragments arranged by size:
5509 960 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2668 2998 4699 5564 5862 2668
Size: 330 1701 865 298 3380
Fragments arranged by size:
3380 1701 865 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2100 2813 5237 882
Size: 617 601 713 2424 2219
Fragments arranged by size:
2424 2219 713 617 601
BsaAI yAC'GTr
Cuts at: 493 1204 3376 3871 4365 493
Size: 711 2172 495 494 2702
Fragments arranged by size:
2702 2172 711 495 494
BsaBI GATnn'nnATC
Cuts at: 6568 6568
Size: 6574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2221 3441 4381 4763
Size: 53 83 186 936 688 1220 940 382
Cuts at: 4763 5398 275
Size: 635 1451
Fragments arranged by size:
1451 1220 940 936 688 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5007 5405 5679 5826 6237 5007
Size: 398 274 147 411 5344
Fragments arranged by size:
5344 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2018 2048 2117 2147 3581
Size: 30 165 30 615 30 69 30 1434
Cuts at: 3581 3611 3827 3857 1178
Size: 30 216 30 3895
Fragments arranged by size:
3895 1434 615 216 165 69 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2777 2945 3602 1186
Size: 1102 489 168 657 4158
Fragments arranged by size:
4158 1102 657 489 168
BseYI C'CCAG_C
Cuts at: 2185 2935 3126 3248 3278 3365 3620 3793 5777
Size: 750 191 122 30 87 255 173 1984
Cuts at: 5777 6273 2185
Size: 496 2486
Fragments arranged by size:
2486 1984 750 496 255 191 173 122
87 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2584 2600 1163
Size: 573 848 16 5137
Fragments arranged by size:
5137 848 573 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4024 4785 5813 1540
Size: 275 186 2023 761 1028 2301
Fragments arranged by size:
2301 2023 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2135 3510 3717 4635 4720 5791 2135
Size: 1375 207 918 85 1071 2918
Fragments arranged by size:
2918 1375 1071 918 207 85
BsmI GAATG_Cn'
Cuts at: 4085 4178 4085
Size: 93 6481
BsmBI CGTCTCn'nnnn_
Cuts at: 2483 3746 2483
Size: 1263 5311
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2482 2576 2672 2752 2830 3065
Size: 399 345 347 94 96 80 78 235
Cuts at: 3065 3104 3147 3155 3276 3510 3609 3638 3717
Size: 39 43 8 121 234 99 29 79
Cuts at: 3717 3764 3893 4635 4720 5791 1391
Size: 47 129 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 347 345 235 234
129 121 99 96 94 85 80 79
78 47 43 39 29 8
BspEI T'CCGG_A
Cuts at: 6237 6237
Size: 6574
BspHI T'CATG_A
Cuts at: 4463 6193 4463
Size: 1730 4844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2505 2622 3929 844
Size: 1661 117 1307 3489
Fragments arranged by size:
3489 1661 1307 117
BsrBI CCG'CTC
Cuts at: 1394 1823 2698 2788 3089 3135 3174 3219 4461
Size: 429 875 90 301 46 39 45 1242
Cuts at: 4461 1394
Size: 3507
Fragments arranged by size:
3507 1242 875 429 301 90 46 45
39
BsrDI GCAATG_nn'
Cuts at: 65 1248 5069 5243 65
Size: 1183 3821 174 1396
Fragments arranged by size:
3821 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2940 3223 3383 5218 5405 1700
Size: 110 1130 283 160 1835 187 2869
Fragments arranged by size:
2869 1835 1130 283 187 160 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6574
BssHII G'CGCG_C
Cuts at: 1925 3346 3541 1925
Size: 1421 195 4958
Fragments arranged by size:
4958 1421 195
BssSI C'ACGA_G
Cuts at: 4634 5646 4634
Size: 1012 5562
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2527 794
Size: 1733 4841
BstBI TT'CG_AA
Cuts at: 2007 5378 2007
Size: 3371 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6574
BstYI r'GATC_y
Cuts at: 1972 2505 2639 2833 3864 3904 4271 4656 4673
Size: 533 134 194 1031 40 367 385 17
Cuts at: 4673 6125 6211 6223 6569 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1031 533 385 367 346 194
134 86 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3536 513
Size: 552 957 1514 3551
Fragments arranged by size:
3551 1514 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5835
Fragments arranged by size:
5835 683 56
BtsI GCAGTG_nn'
Cuts at: 2157 4090 4884 4904 2157
Size: 1933 794 20 3827
Fragments arranged by size:
3827 1933 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 3878 3878
Size: 6574
DraIII CAC_nnn'GTG
Cuts at: 2681 3376 2681
Size: 695 5879
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5581 809
Size: 507 4265 1802
Fragments arranged by size:
4265 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2663 3013 3302
Size: 54 1425 50 275 18 833 350 289
Cuts at: 3302 3339 3471 3728 4021 4910 6539 8
Size: 37 132 257 293 889 1629 43
Fragments arranged by size:
1629 1425 889 833 350 293 289 275
257 132 54 50 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 4021 1537
Size: 275 2209 4090
Fragments arranged by size:
4090 2209 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2438 2706 4504 1353
Size: 1085 268 1798 3423
Fragments arranged by size:
3423 1798 1085 268
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2125 3434 5161 5535 5681 387
Size: 1064 138 536 1309 1727 374 146 1280
Fragments arranged by size:
1727 1309 1280 1064 536 374 146 138
EcoICRI GAG'CTC
Cuts at: 3508 3508
Size: 6574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2339 2531 2714 2756 2825 3872
Size: 393 584 123 192 183 42 69 1047
Cuts at: 3872 4637 5234 6021 6289 1239
Size: 765 597 787 268 1524
Fragments arranged by size:
1524 1047 787 765 597 584 393 268
192 183 123 69 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2318 2060
Size: 258 6316
EcoO109I rG'GnC_Cy
Cuts at: 2068 2075 2191 2478 2479 3525 2068
Size: 7 116 287 1 1046 5117
Fragments arranged by size:
5117 1046 287 116 7 1
EcoRI G'AATT_C
Cuts at: 3883 4261 3883
Size: 378 6196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2417 2467 3403 2417
Size: 50 936 5588
Fragments arranged by size:
5588 936 50
FspI TGC'GCA
Cuts at: 5080 5080
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 2909 3133 3172 3217 3262 3444 5721 1678
Size: 1231 224 39 45 45 182 2277 2531
Fragments arranged by size:
2531 2277 1231 224 182 45 45 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2211 2243 2397
Size: 32 135 32 545 32 62 32 154
Cuts at: 2397 2429 2825 2857 5215 5247 5289 5321 1373
Size: 32 396 32 2358 32 42 32 2626
Fragments arranged by size:
2626 2358 545 396 154 135 62 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3918 4164 669
Size: 988 281 1980 246 3079
Fragments arranged by size:
3079 1980 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6574
HpaI GTT'AAC
Cuts at: 4164 4164
Size: 6574
KasI G'GCGC_C
Cuts at: 3440 3440
Size: 6574
KpnI G_GTAC'C
Cuts at: 3814 3899 3814
Size: 85 6489
MfeI C'AATT_G
Cuts at: 4173 4173
Size: 6574
MluI A'CGCG_T
Cuts at: 6450 6450
Size: 6574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2242 2589 2832 2868 3564 3789 5688 5872
Size: 937 347 243 36 696 225 1899 184
Cuts at: 5872 1305
Size: 2007
Fragments arranged by size:
2007 1899 937 696 347 243 225 184
36
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4752
Fragments arranged by size:
4752 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4532 4891 5050 518
Size: 648 3366 359 159 2042
Fragments arranged by size:
3366 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2527 2812 3031 3282 3538 3645 3777 3793
Size: 921 285 219 251 256 107 132 16
Cuts at: 3793 3990 4667 5815 6060 1606
Size: 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 677 285 256 251 245
219 197 132 107 16
NaeI GCC'GGC
Cuts at: 2942 3385 2942
Size: 443 6131
NarI GG'CG_CC
Cuts at: 3441 3441
Size: 6574
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5065
Fragments arranged by size:
5065 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6574
NgoMIV G'CCGG_C
Cuts at: 2940 3383 2940
Size: 443 6131
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 2131 3932 5477 1132
Size: 97 902 1801 1545 2229
Fragments arranged by size:
2229 1801 1545 902 97
PasI CC'CwG_GG
Cuts at: 1348 2071 1348
Size: 723 5851
PciI A'CATG_T
Cuts at: 2127 5473 2127
Size: 3346 3228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2836 1108
Size: 1728 4846
PfoI T'CCnGG_A
Cuts at: 2101 2547 2757 2101
Size: 446 210 5918
Fragments arranged by size:
5918 446 210
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PmlI CAC'GTG
Cuts at: 3376 3376
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2018 2050 4643 4675 6156 6188 2018
Size: 32 2593 32 1481 32 2404
Fragments arranged by size:
2593 2404 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 4144 4144
Size: 6574
PspOMI G'GGCC_C
Cuts at: 2478 2826 2478
Size: 348 6226
PstI C_TGCA'G
Cuts at: 830 2343 2796 2817 3462 3926 830
Size: 1513 453 21 645 464 3478
Fragments arranged by size:
3478 1513 645 464 453 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 2527 2812 3282 3777 3793 2527
Size: 285 470 495 16 5308
Fragments arranged by size:
5308 495 470 285 16
SacI G_AGCT'C
Cuts at: 3510 3510
Size: 6574
SacII CC_GC'GG
Cuts at: 3539 3539
Size: 6574
SalI G'TCGA_C
Cuts at: 1936 3916 1936
Size: 1980 4594
SbfI CC_TGCA'GG
Cuts at: 3926 3926
Size: 6574
ScaI AGT'ACT
Cuts at: 4822 6534 4822
Size: 1712 4862
SfcI C'TryA_G
Cuts at: 826 1045 2339 2792 2813 2963 3458 3922 5057
Size: 219 1294 453 21 150 495 464 1135
Cuts at: 5057 5738 5929 826
Size: 681 191 1471
Fragments arranged by size:
1471 1294 1135 681 495 464 453 219
191 150 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 3470 3470
Size: 6574
SfoI GGC'GCC
Cuts at: 3442 3442
Size: 6574
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 3223 1700
Size: 1523 5051
SmaI CCC'GGG
Cuts at: 1872 2744 2825 3491 3901 1872
Size: 872 81 666 410 4545
Fragments arranged by size:
4545 872 666 410 81
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2647 2977 4678 5579 5841 820
Size: 197 926 704 330 1701 901 262 1553
Fragments arranged by size:
1701 1553 926 901 704 330 262 197
SnaBI TAC'GTA
Cuts at: 493 3871 4365 493
Size: 3378 494 2702
Fragments arranged by size:
3378 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 3932 1132
Size: 2800 3774
SspI AAT'ATT
Cuts at: 5 52 4498 5
Size: 47 4446 2081
Fragments arranged by size:
4446 2081 47
StuI AGG'CCT
Cuts at: 1517 2420 2544 1517
Size: 903 124 5547
Fragments arranged by size:
5547 903 124
StyI C'CwwG_G
Cuts at: 513 1065 2022 4013 513
Size: 552 957 1991 3074
Fragments arranged by size:
3074 1991 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2692 2860 4601 4618 4771 4956 5401
Size: 1255 1112 168 1741 17 153 185 445
Cuts at: 5401 325
Size: 1498
Fragments arranged by size:
1741 1498 1255 1112 445 185 168 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4820 6532 96
Size: 274 80 33 51 1443 2843 1712 138
Fragments arranged by size:
2843 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3707 3739 3827 3859 6534 6566 3581
Size: 32 94 32 88 32 2675 32 3589
Fragments arranged by size:
3589 2675 94 88 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6574
XbaI T'CTAG_A
Cuts at: 3910 3910
Size: 6574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3628 3628
Size: 6574
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6574
XmaI C'CCGG_G
Cuts at: 1870 2742 2823 3489 3899 1870
Size: 872 81 666 410 4545
Fragments arranged by size:
4545 872 666 410 81
XmnI GAAnn'nnTTC
Cuts at: 4703 4703
Size: 6574
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4382 276
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I
Bsu36I EcoRV FseI FspAI NruI NsiI PacI PpuMI
PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI