Restriction map for
pFN21ASDA0770
(Circular) (Six-base) MAPSORT of: pFN21ASDA0770.seq Check: 1665 from: 1 to: 7354
pFN21ASDA0770 7354 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3538 4501 3538
Size: 963 6391
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5164 278
Size: 53 83 186 936 3628 2468
Fragments arranged by size:
3628 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4697 1937
Size: 2760 4594
Acc65I G'GTAC_C
Cuts at: 4675 4675
Size: 7354
AclI AA'CG_TT
Cuts at: 1563 5481 5854 1563
Size: 3918 373 3063
Fragments arranged by size:
3918 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3719 3732 5417 6801 7069 1632
Size: 2087 13 1685 1384 268 1917
Fragments arranged by size:
2087 1917 1685 1384 268 13
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7157
AflIII A'CryG_T
Cuts at: 1203 6253 7230 1203
Size: 5050 977 1327
Fragments arranged by size:
5050 1327 977
AgeI A'CCGG_T
Cuts at: 2375 6185 2375
Size: 3810 3544
AhdI GACnn_n'nnGTC
Cuts at: 6083 6083
Size: 7354
AleI CACnn'nnGTG
Cuts at: 1166 3122 1166
Size: 1956 5398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2846 3111 1184
Size: 1662 265 5427
Fragments arranged by size:
5427 1662 265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3795 3827 4053 4085 3795
Size: 32 226 32 7064
Fragments arranged by size:
7064 226 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3616 3625 5097 6669 3616
Size: 9 1472 1572 4301
Fragments arranged by size:
4301 1572 1472 9
ApaLI G'TGCA_C
Cuts at: 5411 6567 5411
Size: 1156 6198
ApoI r'AATT_y
Cuts at: 1493 2447 2512 3021 3772 4472 4663 4889 5041
Size: 954 65 509 751 700 191 226 152
Cuts at: 5041 7150 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 954 751 700 509 226 191
152 65
AseI AT'TA_AT
Cuts at: 160 1032 5908 160
Size: 872 4876 1606
Fragments arranged by size:
4876 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7354
AvaI C'yCGr_G
Cuts at: 1870 1943 4313 4679 1870
Size: 73 2370 366 4545
Fragments arranged by size:
4545 2370 366 73
BamHI G'GATC_C
Cuts at: 4684 5051 4684
Size: 367 6987
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4675 6130 618
Size: 325 217 627 2888 1455 1842
Fragments arranged by size:
2888 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2586 2613 3531 4069 4673 1391
Size: 1195 27 918 538 604 4072
Fragments arranged by size:
4072 1195 918 604 538 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6383
BbvCI CC'TCA_GC
Cuts at: 4146 4146
Size: 7354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5545 5579 1903
Size: 34 3608 34 3678
Fragments arranged by size:
3678 3608 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3189 4126 5246 6456 3189
Size: 937 1120 1210 4087
Fragments arranged by size:
4087 1210 1120 937
BclI T'GATC_A
Cuts at: 1548 3292 1548
Size: 1744 5610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3416 5965 136
Size: 107 122 71 631 605 1744 2549 1525
Fragments arranged by size:
2549 1744 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3715 7349 3715
Size: 3634 3720
BlpI GC'TnA_GC
Cuts at: 4771 4771
Size: 7354
Bme1580I G_kGCm'C
Cuts at: 1790 2490 2710 3632 5415 6571 1790
Size: 700 220 922 1783 1156 2573
Fragments arranged by size:
2573 1783 1156 922 700 220
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7354
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2236 3923 5085
Size: 416 53 83 399 24 819 1687 1162
Cuts at: 5085 6043 442
Size: 958 1753
Fragments arranged by size:
1753 1687 1162 958 819 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7354
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3850 3850
Size: 7354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2627 2930 3455 3960 4277 4295 6014
Size: 1106 282 303 525 505 317 18 1719
Cuts at: 6014 1239
Size: 2579
Fragments arranged by size:
2579 1719 1106 525 505 317 303 282
18
Bpu10I CC'TnA_GC
Cuts at: 4146 4146
Size: 7354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3368 4436 5479 6344 6642 3368
Size: 1068 1043 865 298 4080
Fragments arranged by size:
4080 1068 1043 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2366 6017 882
Size: 617 867 3651 2219
Fragments arranged by size:
3651 2219 867 617
BsaAI yAC'GTr
Cuts at: 493 1204 3472 3743 4651 5145 493
Size: 711 2268 271 908 494 2702
Fragments arranged by size:
2702 2268 908 711 494 271
BsaBI GATnn'nnATC
Cuts at: 4200 7348 4200
Size: 3148 4206
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5161 5543 6178 275
Size: 53 83 186 936 3628 382 635 1451
Fragments arranged by size:
3628 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2375 5787 6185 6459 6606 7017 2375
Size: 3412 398 274 147 411 2712
Fragments arranged by size:
3412 2712 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2928 2958 4053 4083 4526
Size: 30 165 30 1525 30 1095 30 443
Cuts at: 4526 4556 4590 4620 1178
Size: 30 34 30 3912
Fragments arranged by size:
3912 1525 1095 443 165 34 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 3473 3839 3971 4163 4547 1186
Size: 1210 1077 366 132 192 384 3993
Fragments arranged by size:
3993 1210 1077 384 366 192 132
BseYI C'CCAG_C
Cuts at: 3934 3958 4235 4636 6557 7053 3934
Size: 24 277 401 1921 496 4235
Fragments arranged by size:
4235 1921 496 401 277 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2677 3629 1163
Size: 573 941 952 4888
Fragments arranged by size:
4888 952 941 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4804 5565 6593 1540
Size: 275 186 2803 761 1028 2301
Fragments arranged by size:
2803 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2586 2745 3201 3471 3555 4069 4452 5415 5500
Size: 159 456 270 84 514 383 963 85
Cuts at: 5500 6571 2586
Size: 1071 3369
Fragments arranged by size:
3369 1071 963 514 456 383 270 159
85 84
BsmI GAATG_Cn'
Cuts at: 4239 4865 4958 4239
Size: 626 93 6635
Fragments arranged by size:
6635 626 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2490 2586 2613 2710 2745 3201 3471
Size: 399 700 96 27 97 35 456 270
Cuts at: 3471 3531 3555 3632 4069 4452 4673 5415 5500
Size: 60 24 77 437 383 221 742 85
Cuts at: 5500 6571 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 700 456 437 399 383
270 221 97 96 85 77 60 35
27 24
BspEI T'CCGG_A
Cuts at: 7017 7017
Size: 7354
BspHI T'CATG_A
Cuts at: 2452 5243 6973 2452
Size: 2791 1730 2833
Fragments arranged by size:
2833 2791 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3079 3153 3538 3648 4297 4501 4709 844
Size: 2235 74 385 110 649 204 208 3489
Fragments arranged by size:
3489 2235 649 385 208 204 110 74
BsrBI CCG'CTC
Cuts at: 1394 1823 3316 4301 4495 5241 1394
Size: 429 1493 985 194 746 3507
Fragments arranged by size:
3507 1493 985 746 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 2760 3188 5849 6023 65
Size: 1183 1512 428 2661 174 1396
Fragments arranged by size:
2661 1512 1396 1183 428 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2375 3969 4020 5998 6185 1700
Size: 110 565 1594 51 1978 187 2869
Fragments arranged by size:
2869 1978 1594 565 187 110 51
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7354
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7354
BssSI C'ACGA_G
Cuts at: 3533 3554 3596 5414 6426 3533
Size: 21 42 1818 1012 4461
Fragments arranged by size:
4461 1818 1012 42 21
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3616 4245 794
Size: 2822 629 3903
Fragments arranged by size:
3903 2822 629
BstBI TT'CG_AA
Cuts at: 2007 6158 2007
Size: 4151 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7354
BstXI CCAn_nnnn'nTGG
Cuts at: 2497 4600 2497
Size: 2103 5251
BstYI r'GATC_y
Cuts at: 1972 2226 2688 2787 3112 3715 4684 5051 5436
Size: 254 462 99 325 603 969 367 385
Cuts at: 5436 5453 6905 6991 7003 7349 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 969 603 462 385 367 346
325 254 99 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2854 4478 2854
Size: 1624 5730
BtgI C'CryG_G
Cuts at: 513 1065 2496 2667 2736 2919 513
Size: 552 1431 171 69 183 4948
Fragments arranged by size:
4948 1431 552 183 171 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6615
Fragments arranged by size:
6615 683 56
BtsI GCAGTG_nn'
Cuts at: 2772 3445 4243 4870 5664 5684 2772
Size: 673 798 627 794 20 4442
Fragments arranged by size:
4442 798 794 673 627 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7354
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7319
DraI TTT'AAA
Cuts at: 4658 4658
Size: 7354
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6361 809
Size: 507 5045 1802
Fragments arranged by size:
5045 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2733 2880 4801
Size: 54 1425 50 275 18 903 147 1921
Cuts at: 4801 5690 7319 8
Size: 889 1629 43
Fragments arranged by size:
1921 1629 1425 903 889 275 147 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4801 1537
Size: 275 2989 4090
Fragments arranged by size:
4090 2989 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3062 3942 4485 5284 1353
Size: 1709 880 543 799 3423
Fragments arranged by size:
3423 1709 880 799 543
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5941 6315 6461 387
Size: 1064 138 4352 374 146 1280
Fragments arranged by size:
4352 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2584 4067 2584
Size: 1483 5871
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2627 2930 3455 3719 3732 3960
Size: 393 713 282 303 525 264 13 228
Cuts at: 3960 4277 4295 5417 6014 6801 7069 1239
Size: 317 18 1122 597 787 268 1524
Fragments arranged by size:
1524 1122 787 713 597 525 393 317
303 282 268 264 228 18 13
EcoNI CCTnn'n_nnAGG
Cuts at: 3588 4403 3588
Size: 815 6539
EcoO109I rG'GnC_Cy
Cuts at: 2838 4309 4348 2838
Size: 1471 39 5844
Fragments arranged by size:
5844 1471 39
EcoRI G'AATT_C
Cuts at: 4472 4663 5041 4472
Size: 191 378 6785
Fragments arranged by size:
6785 378 191
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2147 3244 2147
Size: 1097 6257
FspI TGC'GCA
Cuts at: 5860 5860
Size: 7354
HaeII r_GCGC'y
Cuts at: 1678 6501 1678
Size: 4823 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3284 3316 3820 3852 4590
Size: 32 135 32 1712 32 504 32 738
Cuts at: 4590 4622 5995 6027 6069 6101 1373
Size: 32 1373 32 42 32 2626
Fragments arranged by size:
2626 1712 1373 738 504 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4698 4944 669
Size: 988 281 2760 246 3079
Fragments arranged by size:
3079 2760 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7354
HpaI GTT'AAC
Cuts at: 4944 4944
Size: 7354
KpnI G_GTAC'C
Cuts at: 4679 4679
Size: 7354
MfeI C'AATT_G
Cuts at: 3266 4953 3266
Size: 1687 5667
MluI A'CGCG_T
Cuts at: 7230 7230
Size: 7354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2615 2959 6468 6652 1305
Size: 1310 344 3509 184 2007
Fragments arranged by size:
3509 2007 1310 344 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2735 2882 10
Size: 54 1425 343 903 147 4482
Fragments arranged by size:
4482 1425 903 343 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2701 2729 2765 3122 3402 5312 5671
Size: 648 1535 28 36 357 280 1910 359
Cuts at: 5671 5830 518
Size: 159 2042
Fragments arranged by size:
2042 1910 1535 648 359 357 280 159
36 28
MspA1I CmG'CkG
Cuts at: 1606 2605 3938 4640 4770 5447 6595 6840 1606
Size: 999 1333 702 130 677 1148 245 2120
Fragments arranged by size:
2120 1333 1148 999 702 677 245 130
NaeI GCC'GGC
Cuts at: 4022 4022
Size: 7354
NcoI C'CATG_G
Cuts at: 513 1065 2496 2736 2919 513
Size: 552 1431 240 183 4948
Fragments arranged by size:
4948 1431 552 240 183
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7354
NgoMIV G'CCGG_C
Cuts at: 4020 4020
Size: 7354
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7354
NotI GC'GGCC_GC
Cuts at: 4801 4801
Size: 7354
NspI r_CATG'y
Cuts at: 1132 1229 4712 6257 1132
Size: 97 3483 1545 2229
Fragments arranged by size:
3483 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7354
PciI A'CATG_T
Cuts at: 6253 6253
Size: 7354
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2502 3127 3692 1108
Size: 1394 625 565 4770
Fragments arranged by size:
4770 1394 625 565
PfoI T'CCnGG_A
Cuts at: 2946 3720 4398 4485 2946
Size: 774 678 87 5815
Fragments arranged by size:
5815 774 678 87
PmeI GTTT'AAAC
Cuts at: 4658 4658
Size: 7354
PmlI CAC'GTG
Cuts at: 3472 3743 3472
Size: 271 7083
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4053 4085 5423 5455 6936 6968 4053
Size: 32 1338 32 1481 32 4439
Fragments arranged by size:
4439 1481 1338 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4309 4309
Size: 7354
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7354
PsiI TTA'TAA
Cuts at: 4924 4924
Size: 7354
PspXI vC'TCGA_Gb
Cuts at: 4313 4313
Size: 7354
PstI C_TGCA'G
Cuts at: 830 2424 2976 3549 3621 3919 4308 4706 830
Size: 1594 552 573 72 298 389 398 3478
Fragments arranged by size:
3478 1594 573 552 398 389 298 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7354
PvuII CAG'CTG
Cuts at: 2605 3938 4640 2605
Size: 1333 702 5319
Fragments arranged by size:
5319 1333 702
SacI G_AGCT'C
Cuts at: 2586 4069 2586
Size: 1483 5871
SalI G'TCGA_C
Cuts at: 1936 4696 1936
Size: 2760 4594
SapI GCTCTTCn'nnn_
Cuts at: 4485 4485
Size: 7354
SbfI CC_TGCA'GG
Cuts at: 3919 4706 3919
Size: 787 6567
ScaI AGT'ACT
Cuts at: 4154 5602 7314 4154
Size: 1448 1712 4194
Fragments arranged by size:
4194 1712 1448
SfcI C'TryA_G
Cuts at: 826 1045 2140 2420 2972 3160 3545 3617 3915
Size: 219 1095 280 552 188 385 72 298
Cuts at: 3915 4304 4702 5837 6518 6709 826
Size: 389 398 1135 681 191 1471
Fragments arranged by size:
1471 1135 1095 681 552 398 389 385
298 280 219 191 188 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 3416 3416
Size: 7354
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7354
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7354
SmaI CCC'GGG
Cuts at: 1872 4681 1872
Size: 2809 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3383 4313 4451 5458 6359 6621
Size: 197 926 1440 930 138 1007 901 262
Cuts at: 6621 820
Size: 1553
Fragments arranged by size:
1553 1440 1007 930 926 901 262 197
138
SnaBI TAC'GTA
Cuts at: 493 4651 5145 493
Size: 4158 494 2702
Fragments arranged by size:
4158 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7354
SphI G_CATG'C
Cuts at: 1132 4712 1132
Size: 3580 3774
SspI AAT'ATT
Cuts at: 5 52 5278 5
Size: 47 5226 2081
Fragments arranged by size:
5226 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7354
StyI C'CwwG_G
Cuts at: 513 1065 2496 2736 2919 4344 4793 513
Size: 552 1431 240 183 1425 449 3074
Fragments arranged by size:
3074 1431 1425 552 449 240 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3134 3658 4188 5381 5398 5551 5736
Size: 1255 1554 524 530 1193 17 153 185
Cuts at: 5736 6181 325
Size: 445 1498
Fragments arranged by size:
1554 1498 1255 1193 530 524 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3492 4152 5600
Size: 274 80 33 51 1443 1515 660 1448
Cuts at: 5600 7312 96
Size: 1712 138
Fragments arranged by size:
1712 1515 1448 1443 660 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4526 4558 7314 7346 4526
Size: 32 2756 32 4534
Fragments arranged by size:
4534 2756 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7354
XbaI T'CTAG_A
Cuts at: 4690 4690
Size: 7354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3124 3124
Size: 7354
XhoI C'TCGA_G
Cuts at: 1943 4313 1943
Size: 2370 4984
XmaI C'CCGG_G
Cuts at: 1870 4679 1870
Size: 2809 4545
XmnI GAAnn'nnTTC
Cuts at: 2103 4436 5483 2103
Size: 2333 1047 3974
Fragments arranged by size:
3974 2333 1047
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5162 276
Size: 53 83 186 936 3628 2468
Fragments arranged by size:
3628 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PspOMI PsrI RsrII SacII SanDI
SexAI SfoI SrfI SwaI