Restriction map for
pFN21ASDA0769
(Circular) (Six-base) MAPSORT of: pFN21ASDA0769.seq Check: 9137 from: 1 to: 6781
pFN21ASDA0769 6781 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3281 3281
Size: 6781
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4591 278
Size: 53 83 186 936 3055 2468
Fragments arranged by size:
3055 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4124 1937
Size: 2187 4594
Acc65I G'GTAC_C
Cuts at: 4102 4102
Size: 6781
AclI AA'CG_TT
Cuts at: 1563 4908 5281 1563
Size: 3345 373 3063
Fragments arranged by size:
3345 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 2593 2687 3039 3885 4844 6228 6496
Size: 437 524 94 352 846 959 1384 268
Cuts at: 6496 1632
Size: 1917
Fragments arranged by size:
1917 1384 959 846 524 437 352 268
94
AflII C'TTAA_G
Cuts at: 820 1017 3253 820
Size: 197 2236 4348
Fragments arranged by size:
4348 2236 197
AflIII A'CryG_T
Cuts at: 1203 2852 2854 3498 5680 6657 1203
Size: 1649 2 644 2182 977 1327
Fragments arranged by size:
2182 1649 1327 977 644 2
AgeI A'CCGG_T
Cuts at: 5612 5612
Size: 6781
AhdI GACnn_n'nnGTC
Cuts at: 3490 5510 3490
Size: 2020 4761
AleI CACnn'nnGTG
Cuts at: 1166 2855 4070 1166
Size: 1689 1215 3877
Fragments arranged by size:
3877 1689 1215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6781
AlwNI CAG_nnn'CTG
Cuts at: 2801 3472 4524 6096 2801
Size: 671 1052 1572 3486
Fragments arranged by size:
3486 1572 1052 671
ApaI G_GGCC'C
Cuts at: 3124 3124
Size: 6781
ApaLI G'TGCA_C
Cuts at: 4838 5994 4838
Size: 1156 5625
ApoI r'AATT_y
Cuts at: 1493 3686 4090 4316 4468 6577 1493
Size: 2193 404 226 152 2109 1697
Fragments arranged by size:
2193 2109 1697 404 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5335 160
Size: 872 4303 1606
Fragments arranged by size:
4303 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 4106 1870
Size: 73 183 1980 4545
Fragments arranged by size:
4545 1980 183 73
BamHI G'GATC_C
Cuts at: 4111 4478 4111
Size: 367 6414
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3248 4102 5557 618
Size: 325 217 627 1461 854 1455 1842
Fragments arranged by size:
1842 1461 1455 854 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3124 3878 4100 1391
Size: 1733 754 222 4072
Fragments arranged by size:
4072 1733 754 222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3375 928
Size: 971 1476 4334
Fragments arranged by size:
4334 1476 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4972 5006 1903
Size: 34 3035 34 3678
Fragments arranged by size:
3678 3035 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2115 4673 5883 2115
Size: 2558 1210 3013
Fragments arranged by size:
3013 2558 1210
BclI T'GATC_A
Cuts at: 1548 2581 1548
Size: 1033 5748
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3246 5392 136
Size: 107 122 71 631 605 1574 2146 1525
Fragments arranged by size:
2146 1574 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3780 6776 3780
Size: 2996 3785
BlpI GC'TnA_GC
Cuts at: 4198 4198
Size: 6781
Bme1580I G_kGCm'C
Cuts at: 1790 3124 4842 5998 1790
Size: 1334 1718 1156 2573
Fragments arranged by size:
2573 1718 1334 1156
BmgBI CAC'GTC
Cuts at: 1318 3501 1318
Size: 2183 4598
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2143 2811 3382
Size: 416 53 83 399 24 726 668 571
Cuts at: 3382 4512 5470 442
Size: 1130 958 1753
Fragments arranged by size:
1753 1130 958 726 668 571 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2085 2659 2930 5441 1239
Size: 846 574 271 2511 2579
Fragments arranged by size:
2579 2511 846 574 271
Bpu10I CC'TnA_GC
Cuts at: 3455 3455
Size: 6781
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2573 4906 5771 6069 2573
Size: 2333 865 298 3285
Fragments arranged by size:
3285 2333 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3900 3962 5444 882
Size: 617 2401 62 1482 2219
Fragments arranged by size:
2401 2219 1482 617 62
BsaAI yAC'GTr
Cuts at: 493 1204 2855 2864 4078 4572 493
Size: 711 1651 9 1214 494 2702
Fragments arranged by size:
2702 1651 1214 711 494 9
BsaBI GATnn'nnATC
Cuts at: 3779 6775 3779
Size: 2996 3785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4588 4970 5605 275
Size: 53 83 186 936 3055 382 635 1451
Fragments arranged by size:
3055 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5214 5612 5886 6033 6444 5214
Size: 398 274 147 411 5551
Fragments arranged by size:
5551 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2913 2943 1178
Size: 30 165 30 1510 30 5016
Fragments arranged by size:
5016 1510 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2828 3886 1186
Size: 1642 1058 4081
Fragments arranged by size:
4081 1642 1058
BseYI C'CCAG_C
Cuts at: 2742 3842 3857 5984 6480 2742
Size: 1100 15 2127 496 3043
Fragments arranged by size:
3043 2127 1100 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2663 1163
Size: 573 927 5281
Fragments arranged by size:
5281 927 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4231 4992 6020 1540
Size: 275 186 2230 761 1028 2301
Fragments arranged by size:
2301 2230 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2820 3878 4842 4927 5998 2820
Size: 1058 964 85 1071 3603
Fragments arranged by size:
3603 1071 1058 964 85
BsmI GAATG_Cn'
Cuts at: 4292 4385 4292
Size: 93 6688
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2820 3124 3878 4100 4842 4927 5998
Size: 399 1030 304 754 222 742 85 1071
Cuts at: 5998 1391
Size: 2174
Fragments arranged by size:
2174 1071 1030 754 742 399 304 222
85
BspEI T'CCGG_A
Cuts at: 6444 6444
Size: 6781
BspHI T'CATG_A
Cuts at: 4670 6400 4670
Size: 1730 5051
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2131 3042 3281 4136 844
Size: 1287 911 239 855 3489
Fragments arranged by size:
3489 1287 911 855 239
BsrBI CCG'CTC
Cuts at: 1394 1823 4668 1394
Size: 429 2845 3507
Fragments arranged by size:
3507 2845 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5276 5450 65
Size: 1183 4028 174 1396
Fragments arranged by size:
4028 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5425 5612 1700
Size: 110 3615 187 2869
Fragments arranged by size:
3615 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6781
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6781
BssSI C'ACGA_G
Cuts at: 4841 5853 4841
Size: 1012 5769
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2510 794
Size: 1716 5065
BstBI TT'CG_AA
Cuts at: 2007 5585 2007
Size: 3578 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6781
BstXI CCAn_nnnn'nTGG
Cuts at: 2683 2857 2683
Size: 174 6607
BstYI r'GATC_y
Cuts at: 1972 2262 3780 4111 4478 4863 4880 6332 6418
Size: 290 1518 331 367 385 17 1452 86
Cuts at: 6418 6430 6776 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1518 1452 385 367 346 331 290
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3621 3621
Size: 6781
BtgI C'CryG_G
Cuts at: 513 1065 3513 3761 513
Size: 552 2448 248 3533
Fragments arranged by size:
3533 2448 552 248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3130 487
Size: 683 56 1904 4138
Fragments arranged by size:
4138 1904 683 56
BtsI GCAGTG_nn'
Cuts at: 3141 3818 4297 5091 5111 3141
Size: 677 479 794 20 4811
Fragments arranged by size:
4811 794 677 479 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6781
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3807 3842 529
Size: 35 3243 35 3468
Fragments arranged by size:
3468 3243 35 35
DraI TTT'AAA
Cuts at: 4085 4085
Size: 6781
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5788 809
Size: 507 4472 1802
Fragments arranged by size:
4472 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3397 3474 4228
Size: 54 1425 50 275 18 1567 77 754
Cuts at: 4228 5117 6746 8
Size: 889 1629 43
Fragments arranged by size:
1629 1567 1425 889 754 275 77 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4228 1537
Size: 275 2416 4090
Fragments arranged by size:
4090 2416 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 3189 4711 1353
Size: 692 1144 1522 3423
Fragments arranged by size:
3423 1522 1144 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5368 5742 5888 387
Size: 1064 138 3779 374 146 1280
Fragments arranged by size:
3779 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3876 3876
Size: 6781
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2085 2593 2659 2687 2930 3039
Size: 393 437 16 508 66 28 243 109
Cuts at: 3039 3885 4844 5441 6228 6496 1239
Size: 846 959 597 787 268 1524
Fragments arranged by size:
1524 959 846 787 597 508 437 393
268 243 109 66 28 16
EcoRI G'AATT_C
Cuts at: 3686 4090 4468 3686
Size: 404 378 5999
Fragments arranged by size:
5999 404 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 6781
FspI TGC'GCA
Cuts at: 5287 5287
Size: 6781
HaeII r_GCGC'y
Cuts at: 1678 5928 1678
Size: 4250 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2089 2121 2360 2392 3476
Size: 32 135 32 517 32 239 32 1084
Cuts at: 3476 3508 3596 3618 3628 3650 5422 5454 5496
Size: 32 88 22 10 22 1772 32 42
Cuts at: 5496 5528 1373
Size: 32 2626
Fragments arranged by size:
2626 1772 1084 517 239 135 88 42
32 32 32 32 32 32 32 22
22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2256 2548 3318 4125 4371 669
Size: 988 281 318 292 770 807 246 3079
Fragments arranged by size:
3079 988 807 770 318 292 281 246
HindIII A'AGCT_T
Cuts at: 748 2215 748
Size: 1467 5314
HpaI GTT'AAC
Cuts at: 2548 4371 2548
Size: 1823 4958
KpnI G_GTAC'C
Cuts at: 4106 4106
Size: 6781
MfeI C'AATT_G
Cuts at: 4380 4380
Size: 6781
MluI A'CGCG_T
Cuts at: 6657 6657
Size: 6781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3009 5895 6079 1305
Size: 1704 2886 184 2007
Fragments arranged by size:
2886 2007 1704 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3399 10
Size: 54 1425 343 1567 3392
Fragments arranged by size:
3392 1567 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2855 4070 4739 5098 5257 518
Size: 648 1689 1215 669 359 159 2042
Fragments arranged by size:
2042 1689 1215 669 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2707 4197 4874 6022 6267 1606
Size: 1101 1490 677 1148 245 2120
Fragments arranged by size:
2120 1490 1148 1101 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3761 513
Size: 552 2696 3533
Fragments arranged by size:
3533 2696 552
NdeI CA'TA_TG
Cuts at: 387 3976 387
Size: 3589 3192
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6781
NotI GC'GGCC_GC
Cuts at: 4228 4228
Size: 6781
NspI r_CATG'y
Cuts at: 1132 1229 2104 2634 3463 4139 5684 1132
Size: 97 875 530 829 676 1545 2229
Fragments arranged by size:
2229 1545 875 829 676 530 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6781
PciI A'CATG_T
Cuts at: 5680 5680
Size: 6781
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3094 1108
Size: 1986 4795
PfoI T'CCnGG_A
Cuts at: 2688 2688
Size: 6781
PmeI GTTT'AAAC
Cuts at: 4085 4085
Size: 6781
PmlI CAC'GTG
Cuts at: 2855 2864 2855
Size: 9 6772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4850 4882 6363 6395 4850
Size: 32 1481 32 5236
Fragments arranged by size:
5236 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6781
PsiI TTA'TAA
Cuts at: 4351 4351
Size: 6781
PspOMI G'GGCC_C
Cuts at: 3120 3120
Size: 6781
PstI C_TGCA'G
Cuts at: 830 3136 3468 4133 830
Size: 2306 332 665 3478
Fragments arranged by size:
3478 2306 665 332
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6781
PvuII CAG'CTG
Cuts at: 2707 2707
Size: 6781
SacI G_AGCT'C
Cuts at: 3878 3878
Size: 6781
SalI G'TCGA_C
Cuts at: 1936 4123 1936
Size: 2187 4594
SbfI CC_TGCA'GG
Cuts at: 4133 4133
Size: 6781
ScaI AGT'ACT
Cuts at: 5029 6741 5029
Size: 1712 5069
SfcI C'TryA_G
Cuts at: 826 1045 3132 3428 3464 4129 5264 5945 6136
Size: 219 2087 296 36 665 1135 681 191
Cuts at: 6136 826
Size: 1471
Fragments arranged by size:
2087 1471 1135 681 665 296 219 191
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6781
SmaI CCC'GGG
Cuts at: 1872 4108 1872
Size: 2236 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 2588 3253 4885 5786 6048
Size: 197 926 183 462 665 1632 901 262
Cuts at: 6048 820
Size: 1553
Fragments arranged by size:
1632 1553 926 901 665 462 262 197
183
SnaBI TAC'GTA
Cuts at: 493 4078 4572 493
Size: 3585 494 2702
Fragments arranged by size:
3585 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6781
SphI G_CATG'C
Cuts at: 1132 3463 4139 1132
Size: 2331 676 3774
Fragments arranged by size:
3774 2331 676
SspI AAT'ATT
Cuts at: 5 52 2986 4705 5
Size: 47 2934 1719 2081
Fragments arranged by size:
2934 2081 1719 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6781
StyI C'CwwG_G
Cuts at: 513 1065 3761 4220 513
Size: 552 2696 459 3074
Fragments arranged by size:
3074 2696 552 459
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3480 4808 4825 4978 5163 5608 325
Size: 1255 1900 1328 17 153 185 445 1498
Fragments arranged by size:
1900 1498 1328 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3882 5027 6739
Size: 274 80 33 51 1443 1905 1145 1712
Cuts at: 6739 96
Size: 138
Fragments arranged by size:
1905 1712 1443 1145 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6741 6773 6741
Size: 32 6749
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6781
XbaI T'CTAG_A
Cuts at: 4117 4117
Size: 6781
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 3665 2029
Size: 1636 5145
XhoI C'TCGA_G
Cuts at: 1943 2126 1943
Size: 183 6598
XmaI C'CCGG_G
Cuts at: 1870 4106 1870
Size: 2236 4545
XmnI GAAnn'nnTTC
Cuts at: 2651 2985 4910 2651
Size: 334 1925 4522
Fragments arranged by size:
4522 1925 334
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4589 276
Size: 53 83 186 936 3055 2468
Fragments arranged by size:
3055 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI