Restriction map for
pFN21ASDA0763
(Circular) (Six-base) MAPSORT of: pFN21ASDA0763.seq Check: 7259 from: 1 to: 7252
pFN21ASDA0763 7252 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5062 278
Size: 53 83 186 936 3526 2468
Fragments arranged by size:
3526 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4595 1937
Size: 2658 4594
Acc65I G'GTAC_C
Cuts at: 4573 4573
Size: 7252
AclI AA'CG_TT
Cuts at: 1563 5379 5752 1563
Size: 3816 373 3063
Fragments arranged by size:
3816 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5315 6699 6967 1632
Size: 3683 1384 268 1917
Fragments arranged by size:
3683 1917 1384 268
AfeI AGC'GCT
Cuts at: 2123 3557 2123
Size: 1434 5818
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7055
AflIII A'CryG_T
Cuts at: 1203 3651 6151 7128 1203
Size: 2448 2500 977 1327
Fragments arranged by size:
2500 2448 1327 977
AgeI A'CCGG_T
Cuts at: 6083 6083
Size: 7252
AhdI GACnn_n'nnGTC
Cuts at: 2629 3811 5981 2629
Size: 1182 2170 3900
Fragments arranged by size:
3900 2170 1182
AleI CACnn'nnGTG
Cuts at: 1166 3501 1166
Size: 2335 4917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4448 1184
Size: 3264 3988
AlwNI CAG_nnn'CTG
Cuts at: 2770 4096 4995 6567 2770
Size: 1326 899 1572 3455
Fragments arranged by size:
3455 1572 1326 899
ApaI G_GGCC'C
Cuts at: 2485 2692 2485
Size: 207 7045
ApaLI G'TGCA_C
Cuts at: 2216 5309 6465 2216
Size: 3093 1156 3003
Fragments arranged by size:
3093 3003 1156
ApoI r'AATT_y
Cuts at: 1493 3485 4211 4502 4561 4787 4939 7048 1493
Size: 1992 726 291 59 226 152 2109 1697
Fragments arranged by size:
2109 1992 1697 726 291 226 152 59
AseI AT'TA_AT
Cuts at: 160 1032 5806 160
Size: 872 4774 1606
Fragments arranged by size:
4774 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7252
AvaI C'yCGr_G
Cuts at: 1870 1943 2484 2949 3674 3875 4283 4577 1870
Size: 73 541 465 725 201 408 294 4545
Fragments arranged by size:
4545 725 541 465 408 294 201 73
BamHI G'GATC_C
Cuts at: 3774 3863 4582 4949 3774
Size: 89 719 367 6077
Fragments arranged by size:
6077 719 367 89
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2046 2458 2930 4573 6028
Size: 325 217 627 259 412 472 1643 1455
Cuts at: 6028 618
Size: 1842
Fragments arranged by size:
1842 1643 1455 627 472 412 325 259
217
BanII G_rGCy'C
Cuts at: 1391 2281 2316 2485 2692 3882 4284 4571 1391
Size: 890 35 169 207 1190 402 287 4072
Fragments arranged by size:
4072 1190 890 402 287 207 169 35
BbeI G_GCGC'C
Cuts at: 2050 2934 2050
Size: 884 6368
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6281
BbvCI CC'TCA_GC
Cuts at: 2306 3362 4421 2306
Size: 1056 1059 5137
Fragments arranged by size:
5137 1059 1056
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4370 4404 5443 5477 1903
Size: 34 2433 34 1039 34 3678
Fragments arranged by size:
3678 2433 1039 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5144 6354 5144
Size: 1210 6042
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7252
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2983 5863 136
Size: 107 122 71 631 605 1311 2880 1525
Fragments arranged by size:
2880 1525 1311 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4023 7247 4023
Size: 3224 4028
BlpI GC'TnA_GC
Cuts at: 3016 3155 4669 3016
Size: 139 1514 5599
Fragments arranged by size:
5599 1514 139
Bme1580I G_kGCm'C
Cuts at: 1790 2220 2485 2692 3315 5313 6469 1790
Size: 430 265 207 623 1998 1156 2573
Fragments arranged by size:
2573 1998 1156 623 430 265 207
BmgBI CAC'GTC
Cuts at: 1318 3084 3421 1318
Size: 1766 337 5149
Fragments arranged by size:
5149 1766 337
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2695 4315 4983
Size: 416 53 83 399 24 1278 1620 668
Cuts at: 4983 5941 442
Size: 958 1753
Fragments arranged by size:
1753 1620 1278 958 668 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2326 2393 2771 5912 1239
Size: 1087 67 378 3141 2579
Fragments arranged by size:
3141 2579 1087 378 67
Bpu10I CC'TnA_GC
Cuts at: 2306 3362 4421 2306
Size: 1056 1059 5137
Fragments arranged by size:
5137 1059 1056
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2309 2843 4182 4391 5377 6242 6540 2309
Size: 534 1339 209 986 865 298 3021
Fragments arranged by size:
3021 1339 986 865 534 298 209
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2251 5915 882
Size: 617 752 3664 2219
Fragments arranged by size:
3664 2219 752 617
BsaAI yAC'GTr
Cuts at: 493 1204 4549 5043 493
Size: 711 3345 494 2702
Fragments arranged by size:
3345 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3761 7246 3761
Size: 3485 3767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2047 2358 2379 2931
Size: 53 83 186 936 514 311 21 552
Cuts at: 2931 4299 4465 5059 5441 6076 275
Size: 1368 166 594 382 635 1451
Fragments arranged by size:
1451 1368 936 635 594 552 514 382
311 186 166 83 53 21
BsaWI w'CCGG_w
Cuts at: 3594 4203 5685 6083 6357 6504 6915 3594
Size: 609 1482 398 274 147 411 3931
Fragments arranged by size:
3931 1482 609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2249 2268 2279 2298 2576
Size: 30 165 30 846 19 11 19 278
Cuts at: 2576 2606 2837 2867 2948 2978 3098 3128 1178
Size: 30 231 30 81 30 120 30 5302
Fragments arranged by size:
5302 846 278 231 165 120 81 30
30 30 30 30 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2468 2597 2600 2969 1186
Size: 1282 129 3 369 5469
Fragments arranged by size:
5469 1282 369 129 3
BseYI C'CCAG_C
Cuts at: 2268 2272 2663 2810 3192 4310 6455 6951 2268
Size: 4 391 147 382 1118 2145 496 2569
Fragments arranged by size:
2569 2145 1118 496 391 382 147 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 2495 3842 1163
Size: 573 693 66 1347 4573
Fragments arranged by size:
4573 1347 693 573 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2630 3861 4702 5463 6491 1540
Size: 275 186 629 1231 841 761 1028 2301
Fragments arranged by size:
2301 1231 1028 841 761 629 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 2741 3426 3894 4284 4328 4544 5313 5398
Size: 521 685 468 390 44 216 769 85
Cuts at: 5398 6469 2220
Size: 1071 3003
Fragments arranged by size:
3003 1071 769 685 521 468 390 216
85 44
BsmI GAATG_Cn'
Cuts at: 4763 4856 4763
Size: 93 7159
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2281 2316 2485 2692 2741 3315
Size: 399 430 61 35 169 207 49 574
Cuts at: 3315 3426 3882 3894 4284 4328 4544 4571 5313
Size: 111 456 12 390 44 216 27 742
Cuts at: 5313 5398 6469 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 574 456 430 399 390
216 207 169 111 85 61 49 44
35 27 12
BspEI T'CCGG_A
Cuts at: 3594 6915 3594
Size: 3321 3931
BspHI T'CATG_A
Cuts at: 5141 6871 5141
Size: 1730 5522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 2658 4081 4233 4607 844
Size: 1389 425 1423 152 374 3489
Fragments arranged by size:
3489 1423 1389 425 374 152
BsrBI CCG'CTC
Cuts at: 1394 1823 2755 2841 2848 3532 3679 5139 1394
Size: 429 932 86 7 684 147 1460 3507
Fragments arranged by size:
3507 1460 932 684 429 147 86 7
BsrDI GCAATG_nn'
Cuts at: 65 1248 4236 4501 5747 5921 65
Size: 1183 2988 265 1246 174 1396
Fragments arranged by size:
2988 1396 1246 1183 265 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2461 2625 3024 3366 3396 3927 5896
Size: 110 651 164 399 342 30 531 1969
Cuts at: 5896 6083 1700
Size: 187 2869
Fragments arranged by size:
2869 1969 651 531 399 342 187 164
110 30
BsrGI T'GTAC_A
Cuts at: 96 2571 3654 96
Size: 2475 1083 3694
Fragments arranged by size:
3694 2475 1083
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7252
BssSI C'ACGA_G
Cuts at: 2725 5312 6324 2725
Size: 2587 1012 3653
Fragments arranged by size:
3653 2587 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2770 4096 794
Size: 1976 1326 3950
Fragments arranged by size:
3950 1976 1326
BstBI TT'CG_AA
Cuts at: 2007 6056 2007
Size: 4049 3203
BstEII G'GTnAC_C
Cuts at: 1184 2053 2300 4015 1184
Size: 869 247 1715 4421
Fragments arranged by size:
4421 1715 869 247
BstXI CCAn_nnnn'nTGG
Cuts at: 2685 2989 3466 2685
Size: 304 477 6471
Fragments arranged by size:
6471 477 304
BstYI r'GATC_y
Cuts at: 1972 3762 3774 3863 4023 4582 4949 5334 5351
Size: 1790 12 89 160 559 367 385 17
Cuts at: 5351 6803 6889 6901 7247 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1790 1452 559 385 367 346 160
89 86 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 3479 3479
Size: 7252
BtgI C'CryG_G
Cuts at: 513 1065 3459 513
Size: 552 2394 4306
Fragments arranged by size:
4306 2394 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6513
Fragments arranged by size:
6513 683 56
BtsI GCAGTG_nn'
Cuts at: 4480 4520 4768 5562 5582 4480
Size: 40 248 794 20 6150
Fragments arranged by size:
6150 794 248 40 20
ClaI AT'CG_AT
Cuts at: 1554 2577 1554
Size: 1023 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7217
DraI TTT'AAA
Cuts at: 4556 4556
Size: 7252
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3323 6259 809
Size: 507 2007 2936 1802
Fragments arranged by size:
2936 2007 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2406 2627 3003
Size: 54 1425 50 275 18 576 221 376
Cuts at: 3003 4699 5588 7217 8
Size: 1696 889 1629 43
Fragments arranged by size:
1696 1629 1425 889 576 376 275 221
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2627 4699 1537
Size: 275 815 2072 4090
Fragments arranged by size:
4090 2072 815 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3263 4106 5182 1353
Size: 1910 843 1076 3423
Fragments arranged by size:
3423 1910 1076 843
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5839 6213 6359 387
Size: 1064 138 4250 374 146 1280
Fragments arranged by size:
4250 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4282 4282
Size: 7252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2326 2393 2771 5315 5912 6699 6967
Size: 393 694 67 378 2544 597 787 268
Cuts at: 6967 1239
Size: 1524
Fragments arranged by size:
2544 1524 787 694 597 393 378 268
67
EcoNI CCTnn'n_nnAGG
Cuts at: 2386 2612 4337 2386
Size: 226 1725 5301
Fragments arranged by size:
5301 1725 226
EcoO109I rG'GnC_Cy
Cuts at: 2204 2689 2985 3358 3475 4093 4402 4438 2204
Size: 485 296 373 117 618 309 36 5018
Fragments arranged by size:
5018 618 485 373 309 296 117 36
EcoRI G'AATT_C
Cuts at: 4561 4939 4561
Size: 378 6874
EcoRV GAT'ATC
Cuts at: 4162 4162
Size: 7252
FspI TGC'GCA
Cuts at: 2129 5758 2129
Size: 3629 3623
HaeII r_GCGC'y
Cuts at: 1678 2050 2125 2934 3559 4419 6399 1678
Size: 372 75 809 625 860 1980 2531
Fragments arranged by size:
2531 1980 860 809 625 372 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 2576 2608 2615
Size: 32 135 32 797 32 175 32 7
Cuts at: 2615 2647 2837 2869 3110 3142 3797 3829 4146
Size: 32 190 32 241 32 655 32 317
Cuts at: 4146 4178 5893 5925 5967 5999 1373
Size: 32 1715 32 42 32 2626
Fragments arranged by size:
2626 1715 797 655 317 241 190 175
135 42 32 32 32 32 32 32
32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4596 4842 669
Size: 988 281 2658 246 3079
Fragments arranged by size:
3079 2658 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7252
HpaI GTT'AAC
Cuts at: 4842 4842
Size: 7252
KasI G'GCGC_C
Cuts at: 2046 2930 2046
Size: 884 6368
KpnI G_GTAC'C
Cuts at: 4577 4577
Size: 7252
MfeI C'AATT_G
Cuts at: 4851 4851
Size: 7252
MluI A'CGCG_T
Cuts at: 7128 7128
Size: 7252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2199 2625 3024 3111 3117 4087 4257 6366
Size: 894 426 399 87 6 970 170 2109
Cuts at: 6366 6550 1305
Size: 184 2007
Fragments arranged by size:
2109 2007 970 894 426 399 184 170
87 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3005 10
Size: 54 1425 343 1173 4257
Fragments arranged by size:
4257 1425 1173 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 3501 3650 5210 5569 5728 518
Size: 648 1017 1318 149 1560 359 159 2042
Fragments arranged by size:
2042 1560 1318 1017 648 359 159 149
MspA1I CmG'CkG
Cuts at: 1606 2272 2767 2791 2928 3192 4395 4668 5345
Size: 666 495 24 137 264 1203 273 677
Cuts at: 5345 6493 6738 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1203 1148 677 666 495 273 264
245 137 24
NaeI GCC'GGC
Cuts at: 3368 3929 3368
Size: 561 6691
NarI GG'CG_CC
Cuts at: 2047 2931 2047
Size: 884 6368
NcoI C'CATG_G
Cuts at: 513 1065 3459 513
Size: 552 2394 4306
Fragments arranged by size:
4306 2394 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7252
NgoMIV G'CCGG_C
Cuts at: 3366 3927 3366
Size: 561 6691
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7252
NotI GC'GGCC_GC
Cuts at: 4699 4699
Size: 7252
NspI r_CATG'y
Cuts at: 1132 1229 2155 3655 4090 4318 4610 6155 1132
Size: 97 926 1500 435 228 292 1545 2229
Fragments arranged by size:
2229 1545 1500 926 435 292 228 97
PasI CC'CwG_GG
Cuts at: 1348 3575 1348
Size: 2227 5025
PciI A'CATG_T
Cuts at: 3651 6151 3651
Size: 2500 4752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4255 1108
Size: 3147 4105
PmeI GTTT'AAAC
Cuts at: 4556 4556
Size: 7252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5321 5353 6834 6866 5321
Size: 32 1481 32 5707
Fragments arranged by size:
5707 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4093 4438 4093
Size: 345 6907
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7252
PsiI TTA'TAA
Cuts at: 4822 4822
Size: 7252
PspOMI G'GGCC_C
Cuts at: 2481 2688 2481
Size: 207 7045
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 7220
PstI C_TGCA'G
Cuts at: 830 3113 3351 4527 4604 830
Size: 2283 238 1176 77 3478
Fragments arranged by size:
3478 2283 1176 238 77
PvuI CG_AT'CG
Cuts at: 2001 3861 2001
Size: 1860 5392
PvuII CAG'CTG
Cuts at: 2272 3192 2272
Size: 920 6332
SacI G_AGCT'C
Cuts at: 4284 4284
Size: 7252
SalI G'TCGA_C
Cuts at: 1936 4594 1936
Size: 2658 4594
SapI GCTCTTCn'nnn_
Cuts at: 4106 4106
Size: 7252
SbfI CC_TGCA'GG
Cuts at: 4604 4604
Size: 7252
ScaI AGT'ACT
Cuts at: 4115 5500 7212 4115
Size: 1385 1712 4155
Fragments arranged by size:
4155 1712 1385
SfcI C'TryA_G
Cuts at: 826 1045 3109 3347 4523 4600 5735 6416 6607
Size: 219 2064 238 1176 77 1135 681 191
Cuts at: 6607 826
Size: 1471
Fragments arranged by size:
2064 1471 1176 1135 681 238 219 191
77
SfiI GGCCn_nnn'nGGCC
Cuts at: 2983 2983
Size: 7252
SfoI GGC'GCC
Cuts at: 2048 2932 2048
Size: 884 6368
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7252
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7252
SmaI CCC'GGG
Cuts at: 1872 2486 2951 3877 4579 1872
Size: 614 465 926 702 4545
Fragments arranged by size:
4545 926 702 614 465
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2324 2858 4197 4283 4406 5356
Size: 197 926 381 534 1339 86 123 950
Cuts at: 5356 6257 6519 820
Size: 901 262 1553
Fragments arranged by size:
1553 1339 950 926 901 534 381 262
197 123 86
SnaBI TAC'GTA
Cuts at: 493 4549 5043 493
Size: 4056 494 2702
Fragments arranged by size:
4056 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7252
SphI G_CATG'C
Cuts at: 1132 4090 4610 1132
Size: 2958 520 3774
Fragments arranged by size:
3774 2958 520
SspI AAT'ATT
Cuts at: 5 52 5176 5
Size: 47 5124 2081
Fragments arranged by size:
5124 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7252
StyI C'CwwG_G
Cuts at: 513 1065 2887 3459 3508 4691 513
Size: 552 1822 572 49 1183 3074
Fragments arranged by size:
3074 1822 1183 572 552 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2824 5279 5296 5449 5634 6079 325
Size: 1255 1244 2455 17 153 185 445 1498
Fragments arranged by size:
2455 1498 1255 1244 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2571 3654 4113
Size: 274 80 33 51 1443 594 1083 459
Cuts at: 4113 5498 7210 96
Size: 1385 1712 138
Fragments arranged by size:
1712 1443 1385 1083 594 459 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2268 2300 7212 7244 2268
Size: 32 4912 32 2276
Fragments arranged by size:
4912 2276 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3431 1316
Size: 2115 5137
XbaI T'CTAG_A
Cuts at: 4588 4588
Size: 7252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2368 3616 2368
Size: 1248 6004
XhoI C'TCGA_G
Cuts at: 1943 4283 1943
Size: 2340 4912
XmaI C'CCGG_G
Cuts at: 1870 2484 2949 3875 4577 1870
Size: 614 465 926 702 4545
Fragments arranged by size:
4545 926 702 614 465
XmnI GAAnn'nnTTC
Cuts at: 3257 3407 4211 5381 3257
Size: 150 804 1170 5128
Fragments arranged by size:
5128 1170 804 150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5060 276
Size: 53 83 186 936 3526 2468
Fragments arranged by size:
3526 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI RsrII SacII SanDI SexAI
SrfI SwaI