Restriction map for
pFN21ASDA0758
(Circular) (Six-base) MAPSORT of: pFN21ASDA0758.seq Check: 9935 from: 1 to: 8767
pFN21ASDA0758 8767 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3047 5186 3047
Size: 2139 6628
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2454 3702 6577 278
Size: 53 83 186 936 918 1248 2875 2468
Fragments arranged by size:
2875 2468 1248 936 918 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2108 6110 1937
Size: 171 4002 4594
Fragments arranged by size:
4594 4002 171
Acc65I G'GTAC_C
Cuts at: 6088 6088
Size: 8767
AclI AA'CG_TT
Cuts at: 1563 4095 6894 7267 1563
Size: 2532 2799 373 3063
Fragments arranged by size:
3063 2799 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2223 2504 4289 4848 4882 5855 6830 8214
Size: 591 281 1785 559 34 973 975 1384
Cuts at: 8214 8482 1632
Size: 268 1917
Fragments arranged by size:
1917 1785 1384 975 973 591 559 281
268 34
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8570
AflIII A'CryG_T
Cuts at: 1203 7666 8643 1203
Size: 6463 977 1327
Fragments arranged by size:
6463 1327 977
AgeI A'CCGG_T
Cuts at: 7598 7598
Size: 8767
AhdI GACnn_n'nnGTC
Cuts at: 7496 7496
Size: 8767
AleI CACnn'nnGTG
Cuts at: 1166 5594 5735 5924 1166
Size: 4428 141 189 4009
Fragments arranged by size:
4428 4009 189 141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8767
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2576 2608 2618 2650 2576
Size: 32 10 32 8693
Fragments arranged by size:
8693 32 32 10
AlwNI CAG_nnn'CTG
Cuts at: 6510 8082 6510
Size: 1572 7195
ApaLI G'TGCA_C
Cuts at: 6824 7980 6824
Size: 1156 7611
ApoI r'AATT_y
Cuts at: 1493 2652 3135 3673 4384 6076 6302 6454 8563
Size: 1159 483 538 711 1692 226 152 2109
Cuts at: 8563 1493
Size: 1697
Fragments arranged by size:
2109 1697 1692 1159 711 538 483 226
152
AseI AT'TA_AT
Cuts at: 160 1032 7321 160
Size: 872 6289 1606
Fragments arranged by size:
6289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8767
AvaI C'yCGr_G
Cuts at: 1870 1943 2409 5478 6092 1870
Size: 73 466 3069 614 4545
Fragments arranged by size:
4545 3069 614 466 73
BamHI G'GATC_C
Cuts at: 2254 4033 6097 6464 2254
Size: 1779 2064 367 4557
Fragments arranged by size:
4557 2064 1779 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2466 6088 7543 618
Size: 325 217 627 679 3622 1455 1842
Fragments arranged by size:
3622 1842 1455 679 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3986 4238 6086 1391
Size: 2595 252 1848 4072
Fragments arranged by size:
4072 2595 1848 252
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2120 2414 2446 2580 4879 5314 5782
Size: 971 221 294 32 134 2299 435 468
Cuts at: 5782 5874 928
Size: 92 3821
Fragments arranged by size:
3821 2299 971 468 435 294 221 134
92 32
BbvCI CC'TCA_GC
Cuts at: 5315 5315
Size: 8767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6958 6992 1903
Size: 34 5021 34 3678
Fragments arranged by size:
5021 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6659 7869 6659
Size: 1210 7557
BclI T'GATC_A
Cuts at: 1548 4227 5562 1548
Size: 2679 1335 4753
Fragments arranged by size:
4753 2679 1335
BfrBI ATG'CAT
Cuts at: 2243 3498 2243
Size: 1255 7512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4866 5282 5540
Size: 107 122 71 631 605 3194 416 258
Cuts at: 5540 7378 136
Size: 1838 1525
Fragments arranged by size:
3194 1838 1525 631 605 416 258 122
107 71
BglII A'GATC_T
Cuts at: 8762 8762
Size: 8767
BlpI GC'TnA_GC
Cuts at: 6184 6184
Size: 8767
Bme1580I G_kGCm'C
Cuts at: 1790 2469 3332 6828 7984 1790
Size: 679 863 3496 1156 2573
Fragments arranged by size:
3496 2573 1156 863 679
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3319 4150 5351
Size: 416 53 83 399 24 1902 831 1201
Cuts at: 5351 5527 6498 7456 442
Size: 176 971 958 1753
Fragments arranged by size:
1902 1753 1201 971 958 831 416 399
176 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4257 4257
Size: 8767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3030 4193 7427 1239
Size: 1791 1163 3234 2579
Fragments arranged by size:
3234 2579 1791 1163
Bpu10I CC'TnA_GC
Cuts at: 4218 4752 5315 4218
Size: 534 563 7670
Fragments arranged by size:
7670 563 534
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2765 3293 5116 6892 7757 8055 2765
Size: 528 1823 1776 865 298 3477
Fragments arranged by size:
3477 1823 1776 865 528 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5732 7430 882
Size: 617 4233 1698 2219
Fragments arranged by size:
4233 2219 1698 617
BsaAI yAC'GTr
Cuts at: 493 1204 4405 6064 6558 493
Size: 711 3201 1659 494 2702
Fragments arranged by size:
3201 2702 1659 711 494
BsaBI GATnn'nnATC
Cuts at: 8761 8761
Size: 8767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2451 3699 6574 6956
Size: 53 83 186 936 918 1248 2875 382
Cuts at: 6956 7591 275
Size: 635 1451
Fragments arranged by size:
2875 1451 1248 936 918 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2637 7200 7598 7872 8019 8430 2637
Size: 4563 398 274 147 411 2974
Fragments arranged by size:
4563 2974 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2576 2606 2798 2828 1178
Size: 30 165 30 1173 30 192 30 7117
Fragments arranged by size:
7117 1173 192 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3595 3815 4178 1186
Size: 2409 220 363 5775
Fragments arranged by size:
5775 2409 363 220
BseYI C'CCAG_C
Cuts at: 4100 5461 5472 5554 7970 8466 4100
Size: 1361 11 82 2416 496 4401
Fragments arranged by size:
4401 2416 1361 496 82 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2070 4595 1163
Size: 573 334 2525 5335
Fragments arranged by size:
5335 2525 573 334
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6217 6978 8006 1540
Size: 275 186 4216 761 1028 2301
Fragments arranged by size:
4216 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2384 4410 6828 6913 7984 2384
Size: 2026 2418 85 1071 3167
Fragments arranged by size:
3167 2418 2026 1071 85
BsmI GAATG_Cn'
Cuts at: 6278 6371 6278
Size: 93 8674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2384 2469 3332 3986 4238 4410 6086
Size: 399 594 85 863 654 252 172 1676
Cuts at: 6086 6828 6913 7984 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1676 1071 863 742 654 594 399
252 172 85 85
BspEI T'CCGG_A
Cuts at: 2637 8430 2637
Size: 5793 2974
BspHI T'CATG_A
Cuts at: 2131 2580 6656 8386 2131
Size: 449 4076 1730 2512
Fragments arranged by size:
4076 2512 1730 449
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2368 3047 5186 5382 6122 844
Size: 1524 679 2139 196 740 3489
Fragments arranged by size:
3489 2139 1524 740 679 196
BsrBI CCG'CTC
Cuts at: 1394 1823 6654 1394
Size: 429 4831 3507
Fragments arranged by size:
4831 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2470 4478 4666 5409 7262 7436 65
Size: 1183 1222 2008 188 743 1853 174 1396
Fragments arranged by size:
2008 1853 1396 1222 1183 743 188 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4083 7411 7598 1700
Size: 110 2273 3328 187 2869
Fragments arranged by size:
3328 2869 2273 187 110
BsrGI T'GTAC_A
Cuts at: 96 2776 96
Size: 2680 6087
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8767
BssSI C'ACGA_G
Cuts at: 3577 6827 7839 3577
Size: 3250 1012 4505
Fragments arranged by size:
4505 3250 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4938 794
Size: 4144 4623
BstBI TT'CG_AA
Cuts at: 2007 7571 2007
Size: 5564 3203
BstEII G'GTnAC_C
Cuts at: 1184 5143 1184
Size: 3959 4808
BstYI r'GATC_y
Cuts at: 1972 2254 4033 4273 5029 5610 5830 6097 6464
Size: 282 1779 240 756 581 220 267 367
Cuts at: 6464 6849 6866 8318 8404 8416 8762 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1779 1452 756 581 385 367 346
282 267 240 220 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5615 5615
Size: 8767
BtgI C'CryG_G
Cuts at: 513 1065 4719 513
Size: 552 3654 4561
Fragments arranged by size:
4561 3654 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8028
Fragments arranged by size:
8028 683 56
BtsI GCAGTG_nn'
Cuts at: 3406 3652 3775 4939 6283 7077 7097 3406
Size: 246 123 1164 1344 794 20 5076
Fragments arranged by size:
5076 1344 1164 794 246 123 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8732
DraI TTT'AAA
Cuts at: 6071 6071
Size: 8767
DraIII CAC_nnn'GTG
Cuts at: 2049 2049
Size: 8767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3016 7774 809
Size: 507 1700 4758 1802
Fragments arranged by size:
4758 1802 1700 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3432 6214 7103
Size: 54 1425 50 275 18 1602 2782 889
Cuts at: 7103 8732 8
Size: 1629 43
Fragments arranged by size:
2782 1629 1602 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6214 1537
Size: 275 4402 4090
Fragments arranged by size:
4402 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 4163 4321 5648 6697 1353
Size: 795 2015 158 1327 1049 3423
Fragments arranged by size:
3423 2015 1327 1049 795 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2584 7354 7728 7874 387
Size: 1064 138 995 4770 374 146 1280
Fragments arranged by size:
4770 1280 1064 995 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2504 3030 4193 4289 4848 4882
Size: 393 591 281 526 1163 96 559 34
Cuts at: 4882 5855 6830 7427 8214 8482 1239
Size: 973 975 597 787 268 1524
Fragments arranged by size:
1524 1163 975 973 787 597 591 559
526 393 281 268 96 34
EcoNI CCTnn'n_nnAGG
Cuts at: 2519 3628 4751 2519
Size: 1109 1123 6535
Fragments arranged by size:
6535 1123 1109
EcoO109I rG'GnC_Cy
Cuts at: 2607 5533 5689 2607
Size: 2926 156 5685
Fragments arranged by size:
5685 2926 156
EcoRI G'AATT_C
Cuts at: 3673 6076 6454 3673
Size: 2403 378 5986
Fragments arranged by size:
5986 2403 378
EcoRV GAT'ATC
Cuts at: 4759 4759
Size: 8767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 4459 4763 4903 2638
Size: 1821 304 140 6502
Fragments arranged by size:
6502 1821 304 140
FspI TGC'GCA
Cuts at: 7273 7273
Size: 8767
HaeII r_GCGC'y
Cuts at: 1678 2938 7914 1678
Size: 1260 4976 2531
Fragments arranged by size:
4976 2531 1260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2773 2798 2805 2830 3291
Size: 32 135 32 1201 25 7 25 461
Cuts at: 3291 3323 3571 3603 4169 4201 4346 4378 5137
Size: 32 248 32 566 32 145 32 759
Cuts at: 5137 5169 7408 7440 7482 7514 1373
Size: 32 2239 32 42 32 2626
Fragments arranged by size:
2626 2239 1201 759 566 461 248 145
135 42 32 32 32 32 32 32
32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3868 5901 6111 6357 669
Size: 988 281 1930 2033 210 246 3079
Fragments arranged by size:
3079 2033 1930 988 281 246 210
HindIII A'AGCT_T
Cuts at: 748 5847 748
Size: 5099 3668
HpaI GTT'AAC
Cuts at: 3868 6357 3868
Size: 2489 6278
KpnI G_GTAC'C
Cuts at: 6092 6092
Size: 8767
MfeI C'AATT_G
Cuts at: 6366 6366
Size: 8767
MluI A'CGCG_T
Cuts at: 8643 8643
Size: 8767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2934 4030 4397 4680 7881 8065 1305
Size: 1629 1096 367 283 3201 184 2007
Fragments arranged by size:
3201 2007 1629 1096 367 283 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3434 10
Size: 54 1425 343 1602 5343
Fragments arranged by size:
5343 1602 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2136 2339 3413 3428 5009 5570 5594
Size: 648 970 203 1074 15 1581 561 24
Cuts at: 5594 5735 5924 6725 7084 7243 518
Size: 141 189 801 359 159 2042
Fragments arranged by size:
2042 1581 1074 970 801 648 561 359
203 189 159 141 24 15
MspA1I CmG'CkG
Cuts at: 1606 2147 3715 6183 6860 8008 8253 1606
Size: 541 1568 2468 677 1148 245 2120
Fragments arranged by size:
2468 2120 1568 1148 677 541 245
NcoI C'CATG_G
Cuts at: 513 1065 4719 513
Size: 552 3654 4561
Fragments arranged by size:
4561 3654 552
NdeI CA'TA_TG
Cuts at: 387 2716 3831 387
Size: 2329 1115 5323
Fragments arranged by size:
5323 2329 1115
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8767
NotI GC'GGCC_GC
Cuts at: 6214 6214
Size: 8767
NsiI A_TGCA'T
Cuts at: 2245 3500 2245
Size: 1255 7512
NspI r_CATG'y
Cuts at: 1132 1229 4029 6125 7670 1132
Size: 97 2800 2096 1545 2229
Fragments arranged by size:
2800 2229 2096 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2462 5744 5908 1348
Size: 1114 3282 164 4207
Fragments arranged by size:
4207 3282 1114 164
PciI A'CATG_T
Cuts at: 7666 7666
Size: 8767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2297 3139 3636 5220 1108
Size: 1189 842 497 1584 4655
Fragments arranged by size:
4655 1584 1189 842 497
PfoI T'CCnGG_A
Cuts at: 2250 3259 4761 5055 5244 2250
Size: 1009 1502 294 189 5773
Fragments arranged by size:
5773 1502 1009 294 189
PmeI GTTT'AAAC
Cuts at: 6071 6071
Size: 8767
PmlI CAC'GTG
Cuts at: 4405 4405
Size: 8767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2576 2608 6836 6868 8349 8381 2576
Size: 32 4228 32 1481 32 2962
Fragments arranged by size:
4228 2962 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2607 5689 2607
Size: 3082 5685
PshAI GACnn'nnGTC
Cuts at: 1613 2348 1613
Size: 735 8032
PsiI TTA'TAA
Cuts at: 6337 6337
Size: 8767
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2084 2116 2760 2792 2084
Size: 32 644 32 8059
Fragments arranged by size:
8059 644 32 32
PstI C_TGCA'G
Cuts at: 830 2357 3200 3296 6119 830
Size: 1527 843 96 2823 3478
Fragments arranged by size:
3478 2823 1527 843 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8767
PvuII CAG'CTG
Cuts at: 2147 2147
Size: 8767
SalI G'TCGA_C
Cuts at: 1936 6109 1936
Size: 4173 4594
SapI GCTCTTCn'nnn_
Cuts at: 4321 5648 4321
Size: 1327 7440
SbfI CC_TGCA'GG
Cuts at: 6119 6119
Size: 8767
ScaI AGT'ACT
Cuts at: 3609 7015 8727 3609
Size: 3406 1712 3649
Fragments arranged by size:
3649 3406 1712
SexAI A'CCwGG_T
Cuts at: 5219 5219
Size: 8767
SfcI C'TryA_G
Cuts at: 826 1045 2353 2602 3196 3292 6115 7250 7931
Size: 219 1308 249 594 96 2823 1135 681
Cuts at: 7931 8122 826
Size: 191 1471
Fragments arranged by size:
2823 1471 1308 1135 681 594 249 219
191 96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8767
SmaI CCC'GGG
Cuts at: 1872 5480 6094 1872
Size: 3608 614 4545
Fragments arranged by size:
4545 3608 614
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2744 3308 5095 6871 7772 8034
Size: 197 926 801 564 1787 1776 901 262
Cuts at: 8034 820
Size: 1553
Fragments arranged by size:
1787 1776 1553 926 901 801 564 262
197
SnaBI TAC'GTA
Cuts at: 493 6064 6558 493
Size: 5571 494 2702
Fragments arranged by size:
5571 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8767
SphI G_CATG'C
Cuts at: 1132 6125 1132
Size: 4993 3774
SspI AAT'ATT
Cuts at: 5 52 2221 3256 6691 5
Size: 47 2169 1035 3435 2081
Fragments arranged by size:
3435 2169 2081 1035 47
StuI AGG'CCT
Cuts at: 1517 5620 1517
Size: 4103 4664
StyI C'CwwG_G
Cuts at: 513 1065 2033 4429 4719 5529 6206 513
Size: 552 968 2396 290 810 677 3074
Fragments arranged by size:
3074 2396 968 810 677 552 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2391 2633 4623 4926 5440 5486 6794
Size: 1255 811 242 1990 303 514 46 1308
Cuts at: 6794 6811 6964 7149 7594 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1990 1498 1308 1255 811 514 445 303
242 185 153 46 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2776 3607 7013
Size: 274 80 33 51 1443 799 831 3406
Cuts at: 7013 8725 96
Size: 1712 138
Fragments arranged by size:
3406 1712 1443 831 799 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2176 2208 8727 8759 2176
Size: 32 6519 32 2184
Fragments arranged by size:
6519 2184 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2885 4967 1316
Size: 1569 2082 5116
Fragments arranged by size:
5116 2082 1569
XbaI T'CTAG_A
Cuts at: 4684 6103 4684
Size: 1419 7348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3136 5098 5536 5593 6022 3136
Size: 1962 438 57 429 5881
Fragments arranged by size:
5881 1962 438 429 57
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8767
XmaI C'CCGG_G
Cuts at: 1870 5478 6092 1870
Size: 3608 614 4545
Fragments arranged by size:
4545 3608 614
XmnI GAAnn'nnTTC
Cuts at: 6896 6896
Size: 8767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2452 3700 6575 276
Size: 53 83 186 936 918 1248 2875 2468
Fragments arranged by size:
2875 2468 1248 936 918 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI
BstXI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PspOMI PspXI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI