(Circular) (Six-base) MAPSORT of: pFN21ASDA0757.seq Check: 4712 from: 1 to: 6289 pFN21ASDA0757 6289 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3443 3443 Size: 6289 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4099 278 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2393 3632 1937 Size: 456 1239 4594 Fragments arranged by size: 4594 1239 456 Acc65I G'GTAC_C Cuts at: 2999 3610 2999 Size: 611 5678 AclI AA'CG_TT Cuts at: 1563 4416 4789 1563 Size: 2853 373 3063 Fragments arranged by size: 3063 2853 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2272 2846 4352 5736 6004 1632 Size: 640 574 1506 1384 268 1917 Fragments arranged by size: 1917 1506 1384 640 574 268 AfeI AGC'GCT Cuts at: 3134 3212 3478 3134 Size: 78 266 5945 Fragments arranged by size: 5945 266 78 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6092 AflIII A'CryG_T Cuts at: 1203 5188 6165 1203 Size: 3985 977 1327 Fragments arranged by size: 3985 1327 977 AgeI A'CCGG_T Cuts at: 5120 5120 Size: 6289 AhdI GACnn_n'nnGTC Cuts at: 5018 5018 Size: 6289 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6289 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6289 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2020 2052 3536 3568 2020 Size: 32 1484 32 4741 Fragments arranged by size: 4741 1484 32 32 AlwNI CAG_nnn'CTG Cuts at: 2802 3044 4032 5604 2802 Size: 242 988 1572 3487 Fragments arranged by size: 3487 1572 988 242 ApaI G_GGCC'C Cuts at: 2372 2372 Size: 6289 ApaLI G'TGCA_C Cuts at: 4346 5502 4346 Size: 1156 5133 ApoI r'AATT_y Cuts at: 1493 2051 2342 3598 3824 3976 6085 1493 Size: 558 291 1256 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 558 291 226 152 AseI AT'TA_AT Cuts at: 160 1032 4843 160 Size: 872 3811 1606 Fragments arranged by size: 3811 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 AvaI C'yCGr_G Cuts at: 1870 1943 2773 3614 1870 Size: 73 830 841 4545 Fragments arranged by size: 4545 841 830 73 BamHI G'GATC_C Cuts at: 2188 3619 3986 2188 Size: 1431 367 4491 Fragments arranged by size: 4491 1431 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 2999 3422 3610 5065 Size: 325 217 627 495 717 423 188 1455 Cuts at: 5065 618 Size: 1842 Fragments arranged by size: 1842 1455 717 627 495 423 325 217 188 BanII G_rGCy'C Cuts at: 1391 2240 2275 2372 2481 2520 3608 1391 Size: 849 35 97 109 39 1088 4072 Fragments arranged by size: 4072 1088 849 109 97 39 35 BbeI G_GCGC'C Cuts at: 2286 3426 2286 Size: 1140 5149 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2834 928 Size: 971 935 4383 Fragments arranged by size: 4383 971 935 BbvCI CC'TCA_GC Cuts at: 3473 3473 Size: 6289 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4480 4514 1903 Size: 34 2543 34 3678 Fragments arranged by size: 3678 2543 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2038 2098 4181 5391 2038 Size: 60 2083 1210 2936 Fragments arranged by size: 2936 2083 1210 60 BclI T'GATC_A Cuts at: 1548 1548 Size: 6289 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3299 4900 136 Size: 107 122 71 631 605 1627 1601 1525 Fragments arranged by size: 1627 1601 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6284 6284 Size: 6289 BlpI GC'TnA_GC Cuts at: 2171 2252 3706 2171 Size: 81 1454 4754 Fragments arranged by size: 4754 1454 81 Bme1580I G_kGCm'C Cuts at: 1790 2372 2459 4350 5506 1790 Size: 582 87 1891 1156 2573 Fragments arranged by size: 2573 1891 1156 582 87 BmgBI CAC'GTC Cuts at: 1318 3025 1318 Size: 1707 4582 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2028 2812 3436 Size: 416 53 83 399 24 611 784 624 Cuts at: 3436 4020 4978 442 Size: 584 958 1753 Fragments arranged by size: 1753 958 784 624 611 584 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6289 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2726 2726 Size: 6289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2318 2477 2696 3222 4949 1239 Size: 1079 159 219 526 1727 2579 Fragments arranged by size: 2579 1727 1079 526 219 159 Bpu10I CC'TnA_GC Cuts at: 2426 2448 3473 2426 Size: 22 1025 5242 Fragments arranged by size: 5242 1025 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2867 4414 5279 5577 2867 Size: 1547 865 298 3579 Fragments arranged by size: 3579 1547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2480 2512 4952 882 Size: 617 981 32 2440 2219 Fragments arranged by size: 2440 2219 981 617 32 BsaAI yAC'GTr Cuts at: 493 1204 2080 3586 4080 493 Size: 711 876 1506 494 2702 Fragments arranged by size: 2702 1506 876 711 494 BsaBI GATnn'nnATC Cuts at: 6283 6283 Size: 6289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2283 2649 3423 3465 Size: 53 83 186 936 750 366 774 42 Cuts at: 3465 4096 4478 5113 275 Size: 631 382 635 1451 Fragments arranged by size: 1451 936 774 750 635 631 382 366 186 83 53 42 BsaWI w'CCGG_w Cuts at: 4722 5120 5394 5541 5952 4722 Size: 398 274 147 411 5059 Fragments arranged by size: 5059 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3220 3250 3347 3377 1178 Size: 30 165 30 1817 30 97 30 4090 Fragments arranged by size: 4090 1817 165 97 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2248 3187 1186 Size: 1062 939 4288 Fragments arranged by size: 4288 1062 939 BseYI C'CCAG_C Cuts at: 2452 2972 3251 3263 3558 5492 5988 2452 Size: 520 279 12 295 1934 496 2753 Fragments arranged by size: 2753 1934 520 496 295 279 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2102 2162 2261 2643 3421 3500 1163 Size: 573 366 60 99 382 778 79 3952 Fragments arranged by size: 3952 778 573 382 366 99 79 60 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3086 3346 3739 4500 5528 1540 Size: 275 186 1085 260 393 761 1028 2301 Fragments arranged by size: 2301 1085 1028 761 393 275 260 186 BsiHKAI G_wGCw'C Cuts at: 2240 2481 2520 2682 4350 4435 5506 2240 Size: 241 39 162 1668 85 1071 3023 Fragments arranged by size: 3023 1668 1071 241 162 85 39 BsmI GAATG_Cn' Cuts at: 3800 3893 3800 Size: 93 6196 BsmBI CGTCTCn'nnnn_ Cuts at: 2294 2294 Size: 6289 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2275 2372 2459 2481 2520 2682 Size: 399 450 35 97 87 22 39 162 Cuts at: 2682 3608 4350 4435 5506 1391 Size: 926 742 85 1071 2174 Fragments arranged by size: 2174 1071 926 742 450 399 162 97 87 85 39 35 22 BspEI T'CCGG_A Cuts at: 5952 5952 Size: 6289 BspHI T'CATG_A Cuts at: 3280 4178 5908 3280 Size: 898 1730 3661 Fragments arranged by size: 3661 1730 898 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3443 3644 844 Size: 2599 201 3489 Fragments arranged by size: 3489 2599 201 BsrBI CCG'CTC Cuts at: 1394 1823 2731 4176 1394 Size: 429 908 1445 3507 Fragments arranged by size: 3507 1445 908 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4784 4958 65 Size: 1183 3536 174 1396 Fragments arranged by size: 3536 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4933 5120 1700 Size: 110 3123 187 2869 Fragments arranged by size: 3123 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3033 3576 96 Size: 2937 543 2809 Fragments arranged by size: 2937 2809 543 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6289 BssSI C'ACGA_G Cuts at: 2480 2700 3572 4349 5361 2480 Size: 220 872 777 1012 3408 Fragments arranged by size: 3408 1012 872 777 220 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6289 BstBI TT'CG_AA Cuts at: 2007 5093 2007 Size: 3086 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6289 BstXI CCAn_nnnn'nTGG Cuts at: 2041 2041 Size: 6289 BstYI r'GATC_y Cuts at: 1972 2188 3028 3619 3986 4371 4388 5840 5926 Size: 216 840 591 367 385 17 1452 86 Cuts at: 5926 5938 6284 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 840 591 385 367 346 216 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2330 2922 2938 3295 513 Size: 552 957 308 592 16 357 3507 Fragments arranged by size: 3507 957 592 552 357 308 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2151 487 Size: 683 56 925 4625 Fragments arranged by size: 4625 925 683 56 BtsI GCAGTG_nn' Cuts at: 2457 2626 3805 4599 4619 2457 Size: 169 1179 794 20 4127 Fragments arranged by size: 4127 1179 794 169 20 ClaI AT'CG_AT Cuts at: 1554 2200 1554 Size: 646 5643 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2850 2885 529 Size: 35 2286 35 3933 Fragments arranged by size: 3933 2286 35 35 DraI TTT'AAA Cuts at: 3593 3593 Size: 6289 DraIII CAC_nnn'GTG Cuts at: 2185 3444 2185 Size: 1259 5030 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5296 809 Size: 507 3980 1802 Fragments arranged by size: 3980 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2526 2721 2736 Size: 54 1425 50 275 18 696 195 15 Cuts at: 2736 2925 3083 3736 4625 6254 8 Size: 189 158 653 889 1629 43 Fragments arranged by size: 1629 1425 889 696 653 275 195 189 158 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3083 3736 1537 Size: 275 1271 653 4090 Fragments arranged by size: 4090 1271 653 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2324 2400 4219 1353 Size: 971 76 1819 3423 Fragments arranged by size: 3423 1819 971 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2888 3145 4876 5250 5396 387 Size: 1064 138 1299 257 1731 374 146 1280 Fragments arranged by size: 1731 1299 1280 1064 374 257 146 138 EcoICRI GAG'CTC Cuts at: 2238 2479 2518 2238 Size: 241 39 6009 Fragments arranged by size: 6009 241 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2272 2318 2477 2696 2846 3222 4352 Size: 393 640 46 159 219 150 376 1130 Cuts at: 4352 4949 5736 6004 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1130 787 640 597 393 376 268 219 159 150 46 EcoNI CCTnn'n_nnAGG Cuts at: 2180 2180 Size: 6289 EcoO109I rG'GnC_Cy Cuts at: 2369 3337 2369 Size: 968 5321 EcoRI G'AATT_C Cuts at: 2342 3598 3976 2342 Size: 1256 378 4655 Fragments arranged by size: 4655 1256 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2080 2080 Size: 6289 FspI TGC'GCA Cuts at: 4795 4795 Size: 6289 HaeII r_GCGC'y Cuts at: 1678 2286 3136 3214 3258 3426 3480 5436 1678 Size: 608 850 78 44 168 54 1956 2531 Fragments arranged by size: 2531 1956 850 608 168 78 54 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3101 3133 4930 4962 5004 Size: 32 135 32 1529 32 1797 32 42 Cuts at: 5004 5036 1373 Size: 32 2626 Fragments arranged by size: 2626 1797 1529 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2394 3633 3879 669 Size: 988 281 456 1239 246 3079 Fragments arranged by size: 3079 1239 988 456 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6289 HpaI GTT'AAC Cuts at: 3879 3879 Size: 6289 KasI G'GCGC_C Cuts at: 2282 3422 2282 Size: 1140 5149 KpnI G_GTAC'C Cuts at: 3003 3614 3003 Size: 611 5678 MfeI C'AATT_G Cuts at: 3888 3888 Size: 6289 MluI A'CGCG_T Cuts at: 6165 6165 Size: 6289 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5403 5587 1305 Size: 4098 184 2007 Fragments arranged by size: 4098 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2528 2927 10 Size: 54 1425 343 696 399 3372 Fragments arranged by size: 3372 1425 696 399 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4247 4606 4765 518 Size: 648 3081 359 159 2042 Fragments arranged by size: 3081 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2125 3399 3541 3705 4382 5530 5775 1606 Size: 519 1274 142 164 677 1148 245 2120 Fragments arranged by size: 2120 1274 1148 677 519 245 164 142 NarI GG'CG_CC Cuts at: 2283 3423 2283 Size: 1140 5149 NcoI C'CATG_G Cuts at: 513 1065 2022 2922 513 Size: 552 957 900 3880 Fragments arranged by size: 3880 957 900 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6289 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6289 NotI GC'GGCC_GC Cuts at: 3736 3736 Size: 6289 NspI r_CATG'y Cuts at: 1132 1229 2296 3647 5192 1132 Size: 97 1067 1351 1545 2229 Fragments arranged by size: 2229 1545 1351 1067 97 PasI CC'CwG_GG Cuts at: 1348 3051 1348 Size: 1703 4586 PciI A'CATG_T Cuts at: 5188 5188 Size: 6289 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6289 PmeI GTTT'AAAC Cuts at: 3593 3593 Size: 6289 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4358 4390 5871 5903 4358 Size: 32 1481 32 4744 Fragments arranged by size: 4744 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6289 PsiI TTA'TAA Cuts at: 3859 3859 Size: 6289 PspOMI G'GGCC_C Cuts at: 2368 2368 Size: 6289 PspXI vC'TCGA_Gb Cuts at: 2773 2773 Size: 6289 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2069 2101 2069 Size: 32 6257 PstI C_TGCA'G Cuts at: 830 3194 3641 830 Size: 2364 447 3478 Fragments arranged by size: 3478 2364 447 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6289 PvuII CAG'CTG Cuts at: 3541 3541 Size: 6289 SacI G_AGCT'C Cuts at: 2240 2481 2520 2240 Size: 241 39 6009 Fragments arranged by size: 6009 241 39 SalI G'TCGA_C Cuts at: 1936 2392 3631 1936 Size: 456 1239 4594 Fragments arranged by size: 4594 1239 456 SbfI CC_TGCA'GG Cuts at: 3641 3641 Size: 6289 ScaI AGT'ACT Cuts at: 4537 6249 4537 Size: 1712 4577 SfcI C'TryA_G Cuts at: 826 1045 3190 3637 4772 5453 5644 826 Size: 219 2145 447 1135 681 191 1471 Fragments arranged by size: 2145 1471 1135 681 447 219 191 SfoI GGC'GCC Cuts at: 2284 3424 2284 Size: 1140 5149 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6289 SmaI CCC'GGG Cuts at: 1872 3616 1872 Size: 1744 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2773 2882 4393 5294 5556 820 Size: 197 926 830 109 1511 901 262 1553 Fragments arranged by size: 1553 1511 926 901 830 262 197 109 SnaBI TAC'GTA Cuts at: 493 3586 4080 493 Size: 3093 494 2702 Fragments arranged by size: 3093 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6289 SphI G_CATG'C Cuts at: 1132 2296 3647 1132 Size: 1164 1351 3774 Fragments arranged by size: 3774 1351 1164 SspI AAT'ATT Cuts at: 5 52 4213 5 Size: 47 4161 2081 Fragments arranged by size: 4161 2081 47 StuI AGG'CCT Cuts at: 1517 2831 3272 1517 Size: 1314 441 4534 Fragments arranged by size: 4534 1314 441 StyI C'CwwG_G Cuts at: 513 1065 2022 2739 2922 3728 513 Size: 552 957 717 183 806 3074 Fragments arranged by size: 3074 957 806 717 552 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3455 3572 4316 4333 4486 4671 5116 Size: 1255 1875 117 744 17 153 185 445 Cuts at: 5116 325 Size: 1498 Fragments arranged by size: 1875 1498 1255 744 445 185 153 117 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3033 3576 4535 Size: 274 80 33 51 1443 1056 543 959 Cuts at: 4535 6247 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1056 959 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3220 3252 3347 3353 3379 3385 6249 6281 3220 Size: 32 95 6 26 6 2864 32 3228 Fragments arranged by size: 3228 2864 95 32 32 26 6 6 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6289 XbaI T'CTAG_A Cuts at: 3625 3625 Size: 6289 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2701 2701 Size: 6289 XhoI C'TCGA_G Cuts at: 1943 2773 1943 Size: 830 5459 XmaI C'CCGG_G Cuts at: 1870 3614 1870 Size: 1744 4545 XmnI GAAnn'nnTTC Cuts at: 2342 4418 2342 Size: 2076 4213 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4097 276 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI