Restriction map for
pFN21ASDA0757
(Circular) (Six-base) MAPSORT of: pFN21ASDA0757.seq Check: 4712 from: 1 to: 6289
pFN21ASDA0757 6289 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3443 3443
Size: 6289
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4099 278
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2393 3632 1937
Size: 456 1239 4594
Fragments arranged by size:
4594 1239 456
Acc65I G'GTAC_C
Cuts at: 2999 3610 2999
Size: 611 5678
AclI AA'CG_TT
Cuts at: 1563 4416 4789 1563
Size: 2853 373 3063
Fragments arranged by size:
3063 2853 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2272 2846 4352 5736 6004 1632
Size: 640 574 1506 1384 268 1917
Fragments arranged by size:
1917 1506 1384 640 574 268
AfeI AGC'GCT
Cuts at: 3134 3212 3478 3134
Size: 78 266 5945
Fragments arranged by size:
5945 266 78
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6092
AflIII A'CryG_T
Cuts at: 1203 5188 6165 1203
Size: 3985 977 1327
Fragments arranged by size:
3985 1327 977
AgeI A'CCGG_T
Cuts at: 5120 5120
Size: 6289
AhdI GACnn_n'nnGTC
Cuts at: 5018 5018
Size: 6289
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6289
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2020 2052 3536 3568 2020
Size: 32 1484 32 4741
Fragments arranged by size:
4741 1484 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2802 3044 4032 5604 2802
Size: 242 988 1572 3487
Fragments arranged by size:
3487 1572 988 242
ApaI G_GGCC'C
Cuts at: 2372 2372
Size: 6289
ApaLI G'TGCA_C
Cuts at: 4346 5502 4346
Size: 1156 5133
ApoI r'AATT_y
Cuts at: 1493 2051 2342 3598 3824 3976 6085 1493
Size: 558 291 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 558 291 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4843 160
Size: 872 3811 1606
Fragments arranged by size:
3811 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
AvaI C'yCGr_G
Cuts at: 1870 1943 2773 3614 1870
Size: 73 830 841 4545
Fragments arranged by size:
4545 841 830 73
BamHI G'GATC_C
Cuts at: 2188 3619 3986 2188
Size: 1431 367 4491
Fragments arranged by size:
4491 1431 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 2999 3422 3610 5065
Size: 325 217 627 495 717 423 188 1455
Cuts at: 5065 618
Size: 1842
Fragments arranged by size:
1842 1455 717 627 495 423 325 217
188
BanII G_rGCy'C
Cuts at: 1391 2240 2275 2372 2481 2520 3608 1391
Size: 849 35 97 109 39 1088 4072
Fragments arranged by size:
4072 1088 849 109 97 39 35
BbeI G_GCGC'C
Cuts at: 2286 3426 2286
Size: 1140 5149
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2834 928
Size: 971 935 4383
Fragments arranged by size:
4383 971 935
BbvCI CC'TCA_GC
Cuts at: 3473 3473
Size: 6289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4480 4514 1903
Size: 34 2543 34 3678
Fragments arranged by size:
3678 2543 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2038 2098 4181 5391 2038
Size: 60 2083 1210 2936
Fragments arranged by size:
2936 2083 1210 60
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6289
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3299 4900 136
Size: 107 122 71 631 605 1627 1601 1525
Fragments arranged by size:
1627 1601 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6284 6284
Size: 6289
BlpI GC'TnA_GC
Cuts at: 2171 2252 3706 2171
Size: 81 1454 4754
Fragments arranged by size:
4754 1454 81
Bme1580I G_kGCm'C
Cuts at: 1790 2372 2459 4350 5506 1790
Size: 582 87 1891 1156 2573
Fragments arranged by size:
2573 1891 1156 582 87
BmgBI CAC'GTC
Cuts at: 1318 3025 1318
Size: 1707 4582
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2028 2812 3436
Size: 416 53 83 399 24 611 784 624
Cuts at: 3436 4020 4978 442
Size: 584 958 1753
Fragments arranged by size:
1753 958 784 624 611 584 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2726 2726
Size: 6289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 2477 2696 3222 4949 1239
Size: 1079 159 219 526 1727 2579
Fragments arranged by size:
2579 1727 1079 526 219 159
Bpu10I CC'TnA_GC
Cuts at: 2426 2448 3473 2426
Size: 22 1025 5242
Fragments arranged by size:
5242 1025 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2867 4414 5279 5577 2867
Size: 1547 865 298 3579
Fragments arranged by size:
3579 1547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2480 2512 4952 882
Size: 617 981 32 2440 2219
Fragments arranged by size:
2440 2219 981 617 32
BsaAI yAC'GTr
Cuts at: 493 1204 2080 3586 4080 493
Size: 711 876 1506 494 2702
Fragments arranged by size:
2702 1506 876 711 494
BsaBI GATnn'nnATC
Cuts at: 6283 6283
Size: 6289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2283 2649 3423 3465
Size: 53 83 186 936 750 366 774 42
Cuts at: 3465 4096 4478 5113 275
Size: 631 382 635 1451
Fragments arranged by size:
1451 936 774 750 635 631 382 366
186 83 53 42
BsaWI w'CCGG_w
Cuts at: 4722 5120 5394 5541 5952 4722
Size: 398 274 147 411 5059
Fragments arranged by size:
5059 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3220 3250 3347 3377 1178
Size: 30 165 30 1817 30 97 30 4090
Fragments arranged by size:
4090 1817 165 97 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2248 3187 1186
Size: 1062 939 4288
Fragments arranged by size:
4288 1062 939
BseYI C'CCAG_C
Cuts at: 2452 2972 3251 3263 3558 5492 5988 2452
Size: 520 279 12 295 1934 496 2753
Fragments arranged by size:
2753 1934 520 496 295 279 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2102 2162 2261 2643 3421 3500 1163
Size: 573 366 60 99 382 778 79 3952
Fragments arranged by size:
3952 778 573 382 366 99 79 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3086 3346 3739 4500 5528 1540
Size: 275 186 1085 260 393 761 1028 2301
Fragments arranged by size:
2301 1085 1028 761 393 275 260 186
BsiHKAI G_wGCw'C
Cuts at: 2240 2481 2520 2682 4350 4435 5506 2240
Size: 241 39 162 1668 85 1071 3023
Fragments arranged by size:
3023 1668 1071 241 162 85 39
BsmI GAATG_Cn'
Cuts at: 3800 3893 3800
Size: 93 6196
BsmBI CGTCTCn'nnnn_
Cuts at: 2294 2294
Size: 6289
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2275 2372 2459 2481 2520 2682
Size: 399 450 35 97 87 22 39 162
Cuts at: 2682 3608 4350 4435 5506 1391
Size: 926 742 85 1071 2174
Fragments arranged by size:
2174 1071 926 742 450 399 162 97
87 85 39 35 22
BspEI T'CCGG_A
Cuts at: 5952 5952
Size: 6289
BspHI T'CATG_A
Cuts at: 3280 4178 5908 3280
Size: 898 1730 3661
Fragments arranged by size:
3661 1730 898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 3644 844
Size: 2599 201 3489
Fragments arranged by size:
3489 2599 201
BsrBI CCG'CTC
Cuts at: 1394 1823 2731 4176 1394
Size: 429 908 1445 3507
Fragments arranged by size:
3507 1445 908 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4784 4958 65
Size: 1183 3536 174 1396
Fragments arranged by size:
3536 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4933 5120 1700
Size: 110 3123 187 2869
Fragments arranged by size:
3123 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3033 3576 96
Size: 2937 543 2809
Fragments arranged by size:
2937 2809 543
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6289
BssSI C'ACGA_G
Cuts at: 2480 2700 3572 4349 5361 2480
Size: 220 872 777 1012 3408
Fragments arranged by size:
3408 1012 872 777 220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6289
BstBI TT'CG_AA
Cuts at: 2007 5093 2007
Size: 3086 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6289
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2041
Size: 6289
BstYI r'GATC_y
Cuts at: 1972 2188 3028 3619 3986 4371 4388 5840 5926
Size: 216 840 591 367 385 17 1452 86
Cuts at: 5926 5938 6284 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 840 591 385 367 346 216
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2330 2922 2938 3295 513
Size: 552 957 308 592 16 357 3507
Fragments arranged by size:
3507 957 592 552 357 308 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2151 487
Size: 683 56 925 4625
Fragments arranged by size:
4625 925 683 56
BtsI GCAGTG_nn'
Cuts at: 2457 2626 3805 4599 4619 2457
Size: 169 1179 794 20 4127
Fragments arranged by size:
4127 1179 794 169 20
ClaI AT'CG_AT
Cuts at: 1554 2200 1554
Size: 646 5643
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2850 2885 529
Size: 35 2286 35 3933
Fragments arranged by size:
3933 2286 35 35
DraI TTT'AAA
Cuts at: 3593 3593
Size: 6289
DraIII CAC_nnn'GTG
Cuts at: 2185 3444 2185
Size: 1259 5030
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5296 809
Size: 507 3980 1802
Fragments arranged by size:
3980 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2526 2721 2736
Size: 54 1425 50 275 18 696 195 15
Cuts at: 2736 2925 3083 3736 4625 6254 8
Size: 189 158 653 889 1629 43
Fragments arranged by size:
1629 1425 889 696 653 275 195 189
158 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3083 3736 1537
Size: 275 1271 653 4090
Fragments arranged by size:
4090 1271 653 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2324 2400 4219 1353
Size: 971 76 1819 3423
Fragments arranged by size:
3423 1819 971 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2888 3145 4876 5250 5396 387
Size: 1064 138 1299 257 1731 374 146 1280
Fragments arranged by size:
1731 1299 1280 1064 374 257 146 138
EcoICRI GAG'CTC
Cuts at: 2238 2479 2518 2238
Size: 241 39 6009
Fragments arranged by size:
6009 241 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 2318 2477 2696 2846 3222 4352
Size: 393 640 46 159 219 150 376 1130
Cuts at: 4352 4949 5736 6004 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1130 787 640 597 393 376 268
219 159 150 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2180 2180
Size: 6289
EcoO109I rG'GnC_Cy
Cuts at: 2369 3337 2369
Size: 968 5321
EcoRI G'AATT_C
Cuts at: 2342 3598 3976 2342
Size: 1256 378 4655
Fragments arranged by size:
4655 1256 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 6289
FspI TGC'GCA
Cuts at: 4795 4795
Size: 6289
HaeII r_GCGC'y
Cuts at: 1678 2286 3136 3214 3258 3426 3480 5436 1678
Size: 608 850 78 44 168 54 1956 2531
Fragments arranged by size:
2531 1956 850 608 168 78 54 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3101 3133 4930 4962 5004
Size: 32 135 32 1529 32 1797 32 42
Cuts at: 5004 5036 1373
Size: 32 2626
Fragments arranged by size:
2626 1797 1529 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2394 3633 3879 669
Size: 988 281 456 1239 246 3079
Fragments arranged by size:
3079 1239 988 456 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6289
HpaI GTT'AAC
Cuts at: 3879 3879
Size: 6289
KasI G'GCGC_C
Cuts at: 2282 3422 2282
Size: 1140 5149
KpnI G_GTAC'C
Cuts at: 3003 3614 3003
Size: 611 5678
MfeI C'AATT_G
Cuts at: 3888 3888
Size: 6289
MluI A'CGCG_T
Cuts at: 6165 6165
Size: 6289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5403 5587 1305
Size: 4098 184 2007
Fragments arranged by size:
4098 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2528 2927 10
Size: 54 1425 343 696 399 3372
Fragments arranged by size:
3372 1425 696 399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4247 4606 4765 518
Size: 648 3081 359 159 2042
Fragments arranged by size:
3081 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 3399 3541 3705 4382 5530 5775 1606
Size: 519 1274 142 164 677 1148 245 2120
Fragments arranged by size:
2120 1274 1148 677 519 245 164 142
NarI GG'CG_CC
Cuts at: 2283 3423 2283
Size: 1140 5149
NcoI C'CATG_G
Cuts at: 513 1065 2022 2922 513
Size: 552 957 900 3880
Fragments arranged by size:
3880 957 900 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6289
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6289
NotI GC'GGCC_GC
Cuts at: 3736 3736
Size: 6289
NspI r_CATG'y
Cuts at: 1132 1229 2296 3647 5192 1132
Size: 97 1067 1351 1545 2229
Fragments arranged by size:
2229 1545 1351 1067 97
PasI CC'CwG_GG
Cuts at: 1348 3051 1348
Size: 1703 4586
PciI A'CATG_T
Cuts at: 5188 5188
Size: 6289
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6289
PmeI GTTT'AAAC
Cuts at: 3593 3593
Size: 6289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 5871 5903 4358
Size: 32 1481 32 4744
Fragments arranged by size:
4744 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6289
PsiI TTA'TAA
Cuts at: 3859 3859
Size: 6289
PspOMI G'GGCC_C
Cuts at: 2368 2368
Size: 6289
PspXI vC'TCGA_Gb
Cuts at: 2773 2773
Size: 6289
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2069 2101 2069
Size: 32 6257
PstI C_TGCA'G
Cuts at: 830 3194 3641 830
Size: 2364 447 3478
Fragments arranged by size:
3478 2364 447
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6289
PvuII CAG'CTG
Cuts at: 3541 3541
Size: 6289
SacI G_AGCT'C
Cuts at: 2240 2481 2520 2240
Size: 241 39 6009
Fragments arranged by size:
6009 241 39
SalI G'TCGA_C
Cuts at: 1936 2392 3631 1936
Size: 456 1239 4594
Fragments arranged by size:
4594 1239 456
SbfI CC_TGCA'GG
Cuts at: 3641 3641
Size: 6289
ScaI AGT'ACT
Cuts at: 4537 6249 4537
Size: 1712 4577
SfcI C'TryA_G
Cuts at: 826 1045 3190 3637 4772 5453 5644 826
Size: 219 2145 447 1135 681 191 1471
Fragments arranged by size:
2145 1471 1135 681 447 219 191
SfoI GGC'GCC
Cuts at: 2284 3424 2284
Size: 1140 5149
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6289
SmaI CCC'GGG
Cuts at: 1872 3616 1872
Size: 1744 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2773 2882 4393 5294 5556 820
Size: 197 926 830 109 1511 901 262 1553
Fragments arranged by size:
1553 1511 926 901 830 262 197 109
SnaBI TAC'GTA
Cuts at: 493 3586 4080 493
Size: 3093 494 2702
Fragments arranged by size:
3093 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6289
SphI G_CATG'C
Cuts at: 1132 2296 3647 1132
Size: 1164 1351 3774
Fragments arranged by size:
3774 1351 1164
SspI AAT'ATT
Cuts at: 5 52 4213 5
Size: 47 4161 2081
Fragments arranged by size:
4161 2081 47
StuI AGG'CCT
Cuts at: 1517 2831 3272 1517
Size: 1314 441 4534
Fragments arranged by size:
4534 1314 441
StyI C'CwwG_G
Cuts at: 513 1065 2022 2739 2922 3728 513
Size: 552 957 717 183 806 3074
Fragments arranged by size:
3074 957 806 717 552 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3455 3572 4316 4333 4486 4671 5116
Size: 1255 1875 117 744 17 153 185 445
Cuts at: 5116 325
Size: 1498
Fragments arranged by size:
1875 1498 1255 744 445 185 153 117
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3033 3576 4535
Size: 274 80 33 51 1443 1056 543 959
Cuts at: 4535 6247 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1056 959 543 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3220 3252 3347 3353 3379 3385 6249 6281 3220
Size: 32 95 6 26 6 2864 32 3228
Fragments arranged by size:
3228 2864 95 32 32 26 6 6
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6289
XbaI T'CTAG_A
Cuts at: 3625 3625
Size: 6289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2701 2701
Size: 6289
XhoI C'TCGA_G
Cuts at: 1943 2773 1943
Size: 830 5459
XmaI C'CCGG_G
Cuts at: 1870 3614 1870
Size: 1744 4545
XmnI GAAnn'nnTTC
Cuts at: 2342 4418 2342
Size: 2076 4213
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4097 276
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI