Restriction map for
pFN21ASDA0753
(Circular) (Six-base) MAPSORT of: pFN21ASDA0753.seq Check: 9112 from: 1 to: 7630
pFN21ASDA0753 7630 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5440 278
Size: 53 83 186 936 3904 2468
Fragments arranged by size:
3904 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2541 3307 3592 4973 1937
Size: 604 766 285 1381 4594
Fragments arranged by size:
4594 1381 766 604 285
Acc65I G'GTAC_C
Cuts at: 4951 4951
Size: 7630
AclI AA'CG_TT
Cuts at: 1563 5757 6130 1563
Size: 4194 373 3063
Fragments arranged by size:
4194 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 2228 2600 4023 4844 5693 7077 7345
Size: 458 138 372 1423 821 849 1384 268
Cuts at: 7345 1632
Size: 1917
Fragments arranged by size:
1917 1423 1384 849 821 458 372 268
138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7433
AflIII A'CryG_T
Cuts at: 1203 6529 7506 1203
Size: 5326 977 1327
Fragments arranged by size:
5326 1327 977
AgeI A'CCGG_T
Cuts at: 6461 6461
Size: 7630
AhdI GACnn_n'nnGTC
Cuts at: 6359 6359
Size: 7630
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7630
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3348 1184
Size: 2164 5466
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2766 2798 2766
Size: 32 7598
AlwNI CAG_nnn'CTG
Cuts at: 3830 4889 5373 6945 3830
Size: 1059 484 1572 4515
Fragments arranged by size:
4515 1572 1059 484
ApaLI G'TGCA_C
Cuts at: 5687 6843 5687
Size: 1156 6474
ApoI r'AATT_y
Cuts at: 1493 2587 3189 4904 4939 5165 5317 7426 1493
Size: 1094 602 1715 35 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 1094 602 226 152 35
AseI AT'TA_AT
Cuts at: 160 1032 6184 160
Size: 872 5152 1606
Fragments arranged by size:
5152 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7630
AvaI C'yCGr_G
Cuts at: 1870 1943 4955 1870
Size: 73 3012 4545
Fragments arranged by size:
4545 3012 73
AvrII C'CTAG_G
Cuts at: 2066 2066
Size: 7630
BamHI G'GATC_C
Cuts at: 2761 4540 4960 5327 2761
Size: 1779 420 367 5064
Fragments arranged by size:
5064 1779 420 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2531 3550 3685 4951 6406
Size: 325 217 627 744 1019 135 1266 1455
Cuts at: 6406 618
Size: 1842
Fragments arranged by size:
1842 1455 1266 1019 744 627 325 217
135
BanII G_rGCy'C
Cuts at: 1391 3996 4269 4684 4949 1391
Size: 2605 273 415 265 4072
Fragments arranged by size:
4072 2605 415 273 265
BbeI G_GCGC'C
Cuts at: 3554 3554
Size: 7630
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2359 4529 928
Size: 971 460 2170 4029
Fragments arranged by size:
4029 2170 971 460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2160 2194 2792 2826 4733 4767 4862
Size: 34 223 34 598 34 1907 34 95
Cuts at: 4862 4896 5821 5855 1903
Size: 34 925 34 3678
Fragments arranged by size:
3678 1907 925 598 223 95 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2720 3687 5522 6732 2720
Size: 967 1835 1210 3618
Fragments arranged by size:
3618 1835 1210 967
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7630
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2957 6241 136
Size: 107 122 71 631 605 1285 3284 1525
Fragments arranged by size:
3284 1525 1285 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2572 4290 4342 7625 2572
Size: 1718 52 3283 2577
Fragments arranged by size:
3283 2577 1718 52
BlpI GC'TnA_GC
Cuts at: 5047 5047
Size: 7630
Bme1580I G_kGCm'C
Cuts at: 1790 5691 6847 1790
Size: 3901 1156 2573
Fragments arranged by size:
3901 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7630
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3038 5361 6319
Size: 416 53 83 399 24 1621 2323 958
Cuts at: 6319 442
Size: 1753
Fragments arranged by size:
2323 1753 1621 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7630
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4703 4703
Size: 7630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2506 2672 2822 3773 4367 6290 1239
Size: 1267 166 150 951 594 1923 2579
Fragments arranged by size:
2579 1923 1267 951 594 166 150
Bpu10I CC'TnA_GC
Cuts at: 4309 4309
Size: 7630
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3789 5755 6620 6918 3789
Size: 1966 865 298 4501
Fragments arranged by size:
4501 1966 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3362 6293 882
Size: 617 1863 2931 2219
Fragments arranged by size:
2931 2219 1863 617
BsaAI yAC'GTr
Cuts at: 493 1204 4927 5421 493
Size: 711 3723 494 2702
Fragments arranged by size:
3723 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3342 7624 3342
Size: 4282 3348
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3551 5437 5819 6454
Size: 53 83 186 936 2018 1886 382 635
Cuts at: 6454 275
Size: 1451
Fragments arranged by size:
2018 1886 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3364 6063 6461 6735 6882 7293 3364
Size: 2699 398 274 147 411 3701
Fragments arranged by size:
3701 2699 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7405
Fragments arranged by size:
7405 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2101 2961 3277 4079 4491 1186
Size: 915 860 316 802 412 4325
Fragments arranged by size:
4325 915 860 802 412 316
BseYI C'CCAG_C
Cuts at: 4179 6833 7329 4179
Size: 2654 496 4480
Fragments arranged by size:
4480 2654 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3644 4170 1163
Size: 573 1908 526 4623
Fragments arranged by size:
4623 1908 573 526
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5080 5841 6869 1540
Size: 275 186 3079 761 1028 2301
Fragments arranged by size:
3079 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3996 4269 4684 5691 5776 6847 3996
Size: 273 415 1007 85 1071 4779
Fragments arranged by size:
4779 1071 1007 415 273 85
BsmI GAATG_Cn'
Cuts at: 2829 5141 5234 2829
Size: 2312 93 5225
Fragments arranged by size:
5225 2312 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3729 3729
Size: 7630
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3996 4269 4684 4949 5691 5776 6847
Size: 399 2206 273 415 265 742 85 1071
Cuts at: 6847 1391
Size: 2174
Fragments arranged by size:
2206 2174 1071 742 415 399 273 265
85
BspEI T'CCGG_A
Cuts at: 3364 7293 3364
Size: 3929 3701
BspHI T'CATG_A
Cuts at: 4353 4765 5519 7249 4353
Size: 412 754 1730 4734
Fragments arranged by size:
4734 1730 754 412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2836 4985 844
Size: 1992 2149 3489
Fragments arranged by size:
3489 2149 1992
BsrBI CCG'CTC
Cuts at: 1394 1823 5517 1394
Size: 429 3694 3507
Fragments arranged by size:
3694 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2925 6125 6299 65
Size: 1183 1677 3200 174 1396
Fragments arranged by size:
3200 1677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3660 6274 6461 1700
Size: 110 1850 2614 187 2869
Fragments arranged by size:
2869 2614 1850 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7630
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7630
BssSI C'ACGA_G
Cuts at: 5690 6702 5690
Size: 1012 6618
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7630
BstBI TT'CG_AA
Cuts at: 2007 6434 2007
Size: 4427 3203
BstEII G'GTnAC_C
Cuts at: 1184 2636 1184
Size: 1452 6178
BstXI CCAn_nnnn'nTGG
Cuts at: 2081 2081
Size: 7630
BstYI r'GATC_y
Cuts at: 1972 2572 2761 3799 4290 4342 4540 4960 5327
Size: 600 189 1038 491 52 198 420 367
Cuts at: 5327 5712 5729 7181 7267 7279 7625 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1038 600 491 420 385 367
346 198 189 86 52 17 12
BstZ17I GTA'TAC
Cuts at: 2542 2542
Size: 7630
BtgI C'CryG_G
Cuts at: 513 1065 2022 3477 3689 4790 513
Size: 552 957 1455 212 1101 3353
Fragments arranged by size:
3353 1455 1101 957 552 212
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6891
Fragments arranged by size:
6891 683 56
BtsI GCAGTG_nn'
Cuts at: 5146 5940 5960 5146
Size: 794 20 6816
Fragments arranged by size:
6816 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7630
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7595
DraI TTT'AAA
Cuts at: 4934 4934
Size: 7630
DraIII CAC_nnn'GTG
Cuts at: 2452 2452
Size: 7630
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6637 809
Size: 507 5321 1802
Fragments arranged by size:
5321 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3236 5077 5966
Size: 54 1425 50 275 18 1406 1841 889
Cuts at: 5966 7595 8
Size: 1629 43
Fragments arranged by size:
1841 1629 1425 1406 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5077 1537
Size: 275 3265 4090
Fragments arranged by size:
4090 3265 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2332 2718 3894 4365 4644 4812 5560 1353
Size: 979 386 1176 471 279 168 748 3423
Fragments arranged by size:
3423 1176 979 748 471 386 279 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6217 6591 6737 387
Size: 1064 138 4628 374 146 1280
Fragments arranged by size:
4628 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3994 4267 4682 3994
Size: 273 415 6942
Fragments arranged by size:
6942 415 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2228 2506 2600 2672 2822 3773
Size: 393 458 138 278 94 72 150 951
Cuts at: 3773 4023 4367 4844 5693 6290 7077 7345 1239
Size: 250 344 477 849 597 787 268 1524
Fragments arranged by size:
1524 951 849 787 597 477 458 393
344 278 268 250 150 138 94 72
EcoO109I rG'GnC_Cy
Cuts at: 2070 3267 4569 4633 2070
Size: 1197 1302 64 5067
Fragments arranged by size:
5067 1302 1197 64
EcoRI G'AATT_C
Cuts at: 4904 4939 5317 4904
Size: 35 378 7217
Fragments arranged by size:
7217 378 35
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2327 2561 3327 4111 2327
Size: 234 766 784 5846
Fragments arranged by size:
5846 784 766 234
FspI TGC'GCA
Cuts at: 6136 6136
Size: 7630
HaeII r_GCGC'y
Cuts at: 1678 3554 6777 1678
Size: 1876 3223 2531
Fragments arranged by size:
3223 2531 1876
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3192 3224 4142 4174 4199
Size: 32 135 32 1620 32 918 32 25
Cuts at: 4199 4231 4516 4548 4601 4633 4747 4779 6271
Size: 32 285 32 53 32 114 32 1492
Cuts at: 6271 6303 6345 6377 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1620 1492 918 285 135 114 53
42 32 32 32 32 32 32 32
32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3308 4974 5220 669
Size: 988 281 1370 1666 246 3079
Fragments arranged by size:
3079 1666 1370 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2754 3454 4807 748
Size: 2006 700 1353 3571
Fragments arranged by size:
3571 2006 1353 700
HpaI GTT'AAC
Cuts at: 5220 5220
Size: 7630
KasI G'GCGC_C
Cuts at: 3550 3550
Size: 7630
KpnI G_GTAC'C
Cuts at: 4955 4955
Size: 7630
MfeI C'AATT_G
Cuts at: 4025 5229 4025
Size: 1204 6426
MluI A'CGCG_T
Cuts at: 7506 7506
Size: 7630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4045 4242 6744 6928 1305
Size: 2740 197 2502 184 2007
Fragments arranged by size:
2740 2502 2007 197 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3238 10
Size: 54 1425 343 1406 4402
Fragments arranged by size:
4402 1425 1406 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5588 5947 6106 518
Size: 648 4422 359 159 2042
Fragments arranged by size:
4422 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 4202 5046 5723 6871 7116 1606
Size: 516 2080 844 677 1148 245 2120
Fragments arranged by size:
2120 2080 1148 844 677 516 245
NarI GG'CG_CC
Cuts at: 3551 3551
Size: 7630
NcoI C'CATG_G
Cuts at: 513 1065 2022 3689 513
Size: 552 957 1667 4454
Fragments arranged by size:
4454 1667 957 552
NdeI CA'TA_TG
Cuts at: 387 4423 387
Size: 4036 3594
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7630
NotI GC'GGCC_GC
Cuts at: 5077 5077
Size: 7630
NspI r_CATG'y
Cuts at: 1132 1229 2189 3931 4988 6533 1132
Size: 97 960 1742 1057 1545 2229
Fragments arranged by size:
2229 1742 1545 1057 960 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7630
PciI A'CATG_T
Cuts at: 6529 6529
Size: 7630
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2452 1108
Size: 1344 6286
PfoI T'CCnGG_A
Cuts at: 2608 2859 3141 2608
Size: 251 282 7097
Fragments arranged by size:
7097 282 251
PmeI GTTT'AAAC
Cuts at: 4934 4934
Size: 7630
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2370 2402 5699 5731 7212 7244 2370
Size: 32 3297 32 1481 32 2756
Fragments arranged by size:
3297 2756 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2070 2070
Size: 7630
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7630
PsiI TTA'TAA
Cuts at: 5200 5200
Size: 7630
PstI C_TGCA'G
Cuts at: 830 2127 4982 830
Size: 1297 2855 3478
Fragments arranged by size:
3478 2855 1297
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7630
PvuII CAG'CTG
Cuts at: 2122 4202 2122
Size: 2080 5550
SacI G_AGCT'C
Cuts at: 3996 4269 4684 3996
Size: 273 415 6942
Fragments arranged by size:
6942 415 273
SalI G'TCGA_C
Cuts at: 1936 3306 4972 1936
Size: 1370 1666 4594
Fragments arranged by size:
4594 1666 1370
SapI GCTCTTCn'nnn_
Cuts at: 4812 4812
Size: 7630
SbfI CC_TGCA'GG
Cuts at: 4982 4982
Size: 7630
ScaI AGT'ACT
Cuts at: 2103 5878 7590 2103
Size: 3775 1712 2143
Fragments arranged by size:
3775 2143 1712
SfcI C'TryA_G
Cuts at: 826 1045 2123 3315 4125 4729 4773 4978 6113
Size: 219 1078 1192 810 604 44 205 1135
Cuts at: 6113 6794 6985 826
Size: 681 191 1471
Fragments arranged by size:
1471 1192 1135 1078 810 681 604 219
205 191 44
SfoI GGC'GCC
Cuts at: 3552 3552
Size: 7630
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7630
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7630
SmaI CCC'GGG
Cuts at: 1872 4957 1872
Size: 3085 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3804 5734 6635 6897 820
Size: 197 926 1861 1930 901 262 1553
Fragments arranged by size:
1930 1861 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4927 5421 493
Size: 4434 494 2702
Fragments arranged by size:
4434 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7630
SphI G_CATG'C
Cuts at: 1132 3931 4988 1132
Size: 2799 1057 3774
Fragments arranged by size:
3774 2799 1057
SspI AAT'ATT
Cuts at: 5 52 4138 5554 5
Size: 47 4086 1416 2081
Fragments arranged by size:
4086 2081 1416 47
StuI AGG'CCT
Cuts at: 1517 3224 1517
Size: 1707 5923
StyI C'CwwG_G
Cuts at: 513 1065 2022 2066 3225 3689 3912 5069 513
Size: 552 957 44 1159 464 223 1157 3074
Fragments arranged by size:
3074 1159 1157 957 552 464 223 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2611 2615 3006 4058 4829 5657 5674
Size: 1255 1031 4 391 1052 771 828 17
Cuts at: 5674 5827 6012 6457 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1052 1031 828 771 445 391
185 153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2101 3242 5876
Size: 274 80 33 51 1443 124 1141 2634
Cuts at: 5876 7588 96
Size: 1712 138
Fragments arranged by size:
2634 1712 1443 1141 274 138 124 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2489 2521 2590 2622 7590 7622 2489
Size: 32 69 32 4968 32 2497
Fragments arranged by size:
4968 2497 69 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7630
XbaI T'CTAG_A
Cuts at: 3439 3968 4966 3439
Size: 529 998 6103
Fragments arranged by size:
6103 998 529
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7630
XmaI C'CCGG_G
Cuts at: 1870 4955 1870
Size: 3085 4545
XmnI GAAnn'nnTTC
Cuts at: 5759 5759
Size: 7630
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5438 276
Size: 53 83 186 936 3904 2468
Fragments arranged by size:
3904 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbvCI BfrBI BsiWI
Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI XcmI