Restriction map for
pFN21ASDA0745
(Circular) (Six-base) MAPSORT of: pFN21ASDA0745.seq Check: 3388 from: 1 to: 7444
pFN21ASDA0745 7444 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2433 5254 278
Size: 53 83 186 936 897 2821 2468
Fragments arranged by size:
2821 2468 936 897 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4787 1937
Size: 2850 4594
Acc65I G'GTAC_C
Cuts at: 3759 4765 3759
Size: 1006 6438
AclI AA'CG_TT
Cuts at: 1563 5571 5944 1563
Size: 4008 373 3063
Fragments arranged by size:
4008 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2395 2607 3977 4013 4260 5507 6891 7159
Size: 763 212 1370 36 247 1247 1384 268
Cuts at: 7159 1632
Size: 1917
Fragments arranged by size:
1917 1384 1370 1247 763 268 247 212
36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7247
AflIII A'CryG_T
Cuts at: 1203 2846 6343 7320 1203
Size: 1643 3497 977 1327
Fragments arranged by size:
3497 1643 1327 977
AgeI A'CCGG_T
Cuts at: 6275 6275
Size: 7444
AhdI GACnn_n'nnGTC
Cuts at: 6173 6173
Size: 7444
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2233 1184
Size: 1049 6395
AlwNI CAG_nnn'CTG
Cuts at: 2044 2206 2899 4423 4660 5187 6759 2044
Size: 162 693 1524 237 527 1572 2729
Fragments arranged by size:
2729 1572 1524 693 527 237 162
ApaLI G'TGCA_C
Cuts at: 2850 5501 6657 2850
Size: 2651 1156 3637
Fragments arranged by size:
3637 2651 1156
ApoI r'AATT_y
Cuts at: 1493 2129 4699 4753 4979 5131 7240 1493
Size: 636 2570 54 226 152 2109 1697
Fragments arranged by size:
2570 2109 1697 636 226 152 54
AseI AT'TA_AT
Cuts at: 160 1032 2508 5998 160
Size: 872 1476 3490 1606
Fragments arranged by size:
3490 1606 1476 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
AvaI C'yCGr_G
Cuts at: 1870 1943 4769 1870
Size: 73 2826 4545
Fragments arranged by size:
4545 2826 73
BamHI G'GATC_C
Cuts at: 4774 5141 4774
Size: 367 7077
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3759 4765 6220 618
Size: 325 217 627 1972 1006 1455 1842
Fragments arranged by size:
1972 1842 1455 1006 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3756 4763 1391
Size: 2365 1007 4072
Fragments arranged by size:
4072 2365 1007
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2401 2849 3241 4461 928
Size: 971 502 448 392 1220 3911
Fragments arranged by size:
3911 1220 971 502 448 392
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5635 5669 1903
Size: 34 3698 34 3678
Fragments arranged by size:
3698 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5336 6546 5336
Size: 1210 6234
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7444
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6055 136
Size: 107 122 71 631 605 4383 1525
Fragments arranged by size:
4383 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3153 7439 3153
Size: 4286 3158
BlpI GC'TnA_GC
Cuts at: 3128 4133 4861 3128
Size: 1005 728 5711
Fragments arranged by size:
5711 1005 728
Bme1580I G_kGCm'C
Cuts at: 1790 2854 5505 6661 1790
Size: 1064 2651 1156 2573
Fragments arranged by size:
2651 2573 1156 1064
BmgBI CAC'GTC
Cuts at: 1318 2847 4210 1318
Size: 1529 1363 4552
Fragments arranged by size:
4552 1529 1363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2194 2384 2878
Size: 416 53 83 399 24 777 190 494
Cuts at: 2878 3292 5175 6133 442
Size: 414 1883 958 1753
Fragments arranged by size:
1883 1753 958 777 494 416 414 399
190 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2797 3044 3818 6104 1239
Size: 851 707 247 774 2286 2579
Fragments arranged by size:
2579 2286 851 774 707 247
Bpu10I CC'TnA_GC
Cuts at: 2153 3242 2153
Size: 1089 6355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2666 3776 3947 5569 6434 6732 2666
Size: 1110 171 1622 865 298 3378
Fragments arranged by size:
3378 1622 1110 865 298 171
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3224 3642 6107 882
Size: 617 1725 418 2465 2219
Fragments arranged by size:
2465 2219 1725 617 418
BsaAI yAC'GTr
Cuts at: 493 1204 4331 4741 5235 493
Size: 711 3127 410 494 2702
Fragments arranged by size:
3127 2702 711 494 410
BsaBI GATnn'nnATC
Cuts at: 3528 7438 3528
Size: 3910 3534
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2430 3027 3496 5251
Size: 53 83 186 936 897 597 469 1755
Cuts at: 5251 5633 6268 275
Size: 382 635 1451
Fragments arranged by size:
1755 1451 936 897 635 597 469 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4561 5877 6275 6549 6696 7107 4561
Size: 1316 398 274 147 411 4898
Fragments arranged by size:
4898 1316 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7219
Fragments arranged by size:
7219 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2846 3491 4082 1186
Size: 976 684 645 591 4548
Fragments arranged by size:
4548 976 684 645 591
BseYI C'CCAG_C
Cuts at: 3825 6647 7143 3825
Size: 2822 496 4126
Fragments arranged by size:
4126 2822 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2057 2780 2882 2960 3194 4236 4367
Size: 573 321 723 102 78 234 1042 131
Cuts at: 4367 1163
Size: 4240
Fragments arranged by size:
4240 1042 723 573 321 234 131 102
78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2315 4375 4894 5655 6683 1540
Size: 275 186 314 2060 519 761 1028 2301
Fragments arranged by size:
2301 2060 1028 761 519 314 275 186
BsiHKAI G_wGCw'C
Cuts at: 2295 2854 3060 3417 3756 5505 5590 6661 2295
Size: 559 206 357 339 1749 85 1071 3078
Fragments arranged by size:
3078 1749 1071 559 357 339 206 85
BsmI GAATG_Cn'
Cuts at: 4667 4955 5048 4667
Size: 288 93 7063
Fragments arranged by size:
7063 288 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3503 4041 4043 3503
Size: 538 2 6904
Fragments arranged by size:
6904 538 2
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2295 2854 3060 3417 3756 4763 5505
Size: 399 505 559 206 357 339 1007 742
Cuts at: 5505 5590 6661 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1007 742 559 505 399 357
339 206 85
BspEI T'CCGG_A
Cuts at: 7107 7107
Size: 7444
BspHI T'CATG_A
Cuts at: 3693 5333 7063 3693
Size: 1640 1730 4074
Fragments arranged by size:
4074 1730 1640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4799 844
Size: 3955 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5331 1394
Size: 429 3508 3507
Fragments arranged by size:
3508 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2931 4729 5939 6113 65
Size: 1183 1683 1798 1210 174 1396
Fragments arranged by size:
1798 1683 1396 1210 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6088 6275 1700
Size: 110 4278 187 2869
Fragments arranged by size:
4278 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2753 96
Size: 2657 4787
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7444
BssSI C'ACGA_G
Cuts at: 2858 3406 5504 6516 2858
Size: 548 2098 1012 3786
Fragments arranged by size:
3786 2098 1012 548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2665 3547 4278 4390 4579 794
Size: 1871 882 731 112 189 3659
Fragments arranged by size:
3659 1871 882 731 189 112
BstBI TT'CG_AA
Cuts at: 2007 6248 2007
Size: 4241 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7444
BstYI r'GATC_y
Cuts at: 1972 3153 4774 5141 5526 5543 6995 7081 7093
Size: 1181 1621 367 385 17 1452 86 12
Cuts at: 7093 7439 1972
Size: 346 1977
Fragments arranged by size:
1977 1621 1452 1181 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3030 3234 4263 4428 513
Size: 552 957 1008 204 1029 165 3529
Fragments arranged by size:
3529 1029 1008 957 552 204 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6705
Fragments arranged by size:
6705 683 56
BtsI GCAGTG_nn'
Cuts at: 2943 2988 4960 5754 5774 2943
Size: 45 1972 794 20 4613
Fragments arranged by size:
4613 1972 794 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7409
DraI TTT'AAA
Cuts at: 4748 4748
Size: 7444
DraIII CAC_nnn'GTG
Cuts at: 4303 4303
Size: 7444
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6451 809
Size: 507 5135 1802
Fragments arranged by size:
5135 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2312 2992 3120
Size: 54 1425 50 275 18 482 680 128
Cuts at: 3120 4891 5780 7409 8
Size: 1771 889 1629 43
Fragments arranged by size:
1771 1629 1425 889 680 482 275 128
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2312 4891 1537
Size: 275 500 2579 4090
Fragments arranged by size:
4090 2579 500 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2563 3853 3875 4234 4319 4337 5374 1353
Size: 1210 1290 22 359 85 18 1037 3423
Fragments arranged by size:
3423 1290 1210 1037 359 85 22 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 6031 6405 6551 387
Size: 1064 138 453 3989 374 146 1280
Fragments arranged by size:
3989 1280 1064 453 374 146 138
EcoICRI GAG'CTC
Cuts at: 3754 3754
Size: 7444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2395 2607 2797 3044 3818 3977
Size: 393 458 305 212 190 247 774 159
Cuts at: 3977 4013 4260 5507 6104 6891 7159 1239
Size: 36 247 1247 597 787 268 1524
Fragments arranged by size:
1524 1247 787 774 597 458 393 305
268 247 247 212 190 159 36
EcoNI CCTnn'n_nnAGG
Cuts at: 4169 4361 4169
Size: 192 7252
EcoO109I rG'GnC_Cy
Cuts at: 3822 3931 3822
Size: 109 7335
EcoRI G'AATT_C
Cuts at: 4699 4753 5131 4699
Size: 54 378 7012
Fragments arranged by size:
7012 378 54
EcoRV GAT'ATC
Cuts at: 2587 2587
Size: 7444
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2208 3706 2208
Size: 1498 5946
FspI TGC'GCA
Cuts at: 5950 5950
Size: 7444
HaeII r_GCGC'y
Cuts at: 1678 2913 6591 1678
Size: 1235 3678 2531
Fragments arranged by size:
3678 2531 1235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2723 2755 2970 3002 6085
Size: 32 135 32 1151 32 215 32 3083
Cuts at: 6085 6117 6159 6191 1373
Size: 32 42 32 2626
Fragments arranged by size:
3083 2626 1151 215 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4684 4788 5034 669
Size: 988 281 2746 104 246 3079
Fragments arranged by size:
3079 2746 988 281 246 104
HindIII A'AGCT_T
Cuts at: 748 2228 3371 748
Size: 1480 1143 4821
Fragments arranged by size:
4821 1480 1143
HpaI GTT'AAC
Cuts at: 5034 5034
Size: 7444
KpnI G_GTAC'C
Cuts at: 3763 4769 3763
Size: 1006 6438
MfeI C'AATT_G
Cuts at: 3747 5043 3747
Size: 1296 6148
MluI A'CGCG_T
Cuts at: 7320 7320
Size: 7444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3449 3471 3763 6558 6742 1305
Size: 2144 22 292 2795 184 2007
Fragments arranged by size:
2795 2144 2007 292 184 22
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3122 10
Size: 54 1425 343 1290 4332
Fragments arranged by size:
4332 1425 1290 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2936 5402 5761 5920 518
Size: 648 1770 2466 359 159 2042
Fragments arranged by size:
2466 2042 1770 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2775 2812 3064 4324 4860 5537 6685
Size: 468 701 37 252 1260 536 677 1148
Cuts at: 6685 6930 1606
Size: 245 2120
Fragments arranged by size:
2120 1260 1148 701 677 536 468 252
245 37
NcoI C'CATG_G
Cuts at: 513 1065 2022 3030 3234 4263 513
Size: 552 957 1008 204 1029 3694
Fragments arranged by size:
3694 1029 1008 957 552 204
NdeI CA'TA_TG
Cuts at: 387 4179 387
Size: 3792 3652
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7444
NotI GC'GGCC_GC
Cuts at: 4891 4891
Size: 7444
NruI TCG'CGA
Cuts at: 2568 2568
Size: 7444
NspI r_CATG'y
Cuts at: 1132 1229 2220 3463 4802 6347 1132
Size: 97 991 1243 1339 1545 2229
Fragments arranged by size:
2229 1545 1339 1243 991 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7444
PciI A'CATG_T
Cuts at: 6343 6343
Size: 7444
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2206 2775 4493 1108
Size: 1098 569 1718 4059
Fragments arranged by size:
4059 1718 1098 569
PmeI GTTT'AAAC
Cuts at: 4748 4748
Size: 7444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5513 5545 7026 7058 5513
Size: 32 1481 32 5899
Fragments arranged by size:
5899 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3822 3931 3822
Size: 109 7335
PshAI GACnn'nnGTC
Cuts at: 1613 3236 1613
Size: 1623 5821
PsiI TTA'TAA
Cuts at: 5014 5014
Size: 7444
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 7412
PstI C_TGCA'G
Cuts at: 830 4074 4796 830
Size: 3244 722 3478
Fragments arranged by size:
3478 3244 722
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7444
PvuII CAG'CTG
Cuts at: 2074 2775 2812 3064 4324 2074
Size: 701 37 252 1260 5194
Fragments arranged by size:
5194 1260 701 252 37
SacI G_AGCT'C
Cuts at: 3756 3756
Size: 7444
SalI G'TCGA_C
Cuts at: 1936 4786 1936
Size: 2850 4594
SbfI CC_TGCA'GG
Cuts at: 4796 4796
Size: 7444
ScaI AGT'ACT
Cuts at: 3281 5692 7404 3281
Size: 2411 1712 3321
Fragments arranged by size:
3321 2411 1712
SfcI C'TryA_G
Cuts at: 826 1045 2903 3317 4070 4459 4792 5927 6608
Size: 219 1858 414 753 389 333 1135 681
Cuts at: 6608 6799 826
Size: 191 1471
Fragments arranged by size:
1858 1471 1135 753 681 414 389 333
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7444
SmaI CCC'GGG
Cuts at: 1872 4771 1872
Size: 2899 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2681 3791 3962 5548 6449 6711
Size: 197 926 738 1110 171 1586 901 262
Cuts at: 6711 820
Size: 1553
Fragments arranged by size:
1586 1553 1110 926 901 738 262 197
171
SnaBI TAC'GTA
Cuts at: 493 4741 5235 493
Size: 4248 494 2702
Fragments arranged by size:
4248 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7444
SphI G_CATG'C
Cuts at: 1132 4802 1132
Size: 3670 3774
SspI AAT'ATT
Cuts at: 5 52 5368 5
Size: 47 5316 2081
Fragments arranged by size:
5316 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7444
StyI C'CwwG_G
Cuts at: 513 1065 2022 2121 3030 3234 4263 4883 513
Size: 552 957 99 909 204 1029 620 3074
Fragments arranged by size:
3074 1029 957 909 620 552 204 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2540 4667 5471 5488 5641 5826 6271
Size: 1255 960 2127 804 17 153 185 445
Cuts at: 6271 325
Size: 1498
Fragments arranged by size:
2127 1498 1255 960 804 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2753 3279 3776
Size: 274 80 33 51 1443 776 526 497
Cuts at: 3776 3787 5690 7402 96
Size: 11 1903 1712 138
Fragments arranged by size:
1903 1712 1443 776 526 497 274 138
80 51 33 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2512 2544 7404 7436 2512
Size: 32 4860 32 2520
Fragments arranged by size:
4860 2520 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2405 1316
Size: 1089 6355
XbaI T'CTAG_A
Cuts at: 4780 4780
Size: 7444
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7444
XmaI C'CCGG_G
Cuts at: 1870 4769 1870
Size: 2899 4545
XmnI GAAnn'nnTTC
Cuts at: 3144 3810 5573 3144
Size: 666 1763 5015
Fragments arranged by size:
5015 1763 666
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2431 5252 276
Size: 53 83 186 936 897 2821 2468
Fragments arranged by size:
2821 2468 936 897 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I FseI
FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI
PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI