Restriction map for
pFN21ASDA0742
(Circular) (Six-base) MAPSORT of: pFN21ASDA0742.seq Check: 46 from: 1 to: 8692
pFN21ASDA0742 8692 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3383 3383
Size: 8692
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6502 278
Size: 53 83 186 936 4966 2468
Fragments arranged by size:
4966 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2262 5034 6035 1937
Size: 325 2772 1001 4594
Fragments arranged by size:
4594 2772 1001 325
Acc65I G'GTAC_C
Cuts at: 6013 6013
Size: 8692
AclI AA'CG_TT
Cuts at: 1563 3849 6819 7192 1563
Size: 2286 2970 373 3063
Fragments arranged by size:
3063 2970 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 3105 3429 3468 3724 4990 5370 5826
Size: 593 880 324 39 256 1266 380 456
Cuts at: 5826 6755 8139 8407 1632
Size: 929 1384 268 1917
Fragments arranged by size:
1917 1384 1266 929 880 593 456 380
324 268 256 39
AflII C'TTAA_G
Cuts at: 820 1017 5156 5462 820
Size: 197 4139 306 4050
Fragments arranged by size:
4139 4050 306 197
AflIII A'CryG_T
Cuts at: 1203 2771 7591 8568 1203
Size: 1568 4820 977 1327
Fragments arranged by size:
4820 1568 1327 977
AgeI A'CCGG_T
Cuts at: 7523 7523
Size: 8692
AhdI GACnn_n'nnGTC
Cuts at: 7421 7421
Size: 8692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2872 5568 1184
Size: 1688 2696 4308
Fragments arranged by size:
4308 2696 1688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4218 4250 4218
Size: 32 8660
AlwNI CAG_nnn'CTG
Cuts at: 4412 6435 8007 4412
Size: 2023 1572 5097
Fragments arranged by size:
5097 2023 1572
ApaI G_GGCC'C
Cuts at: 5315 5315
Size: 8692
ApaLI G'TGCA_C
Cuts at: 6749 7905 6749
Size: 1156 7536
ApoI r'AATT_y
Cuts at: 1493 2219 2331 2528 2610 3018 3057 3165 3469
Size: 726 112 197 82 408 39 108 304
Cuts at: 3469 4690 5266 5424 5582 5858 6001 6227 6379
Size: 1221 576 158 158 276 143 226 152
Cuts at: 6379 8488 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1221 726 576 408 304 276
226 197 158 158 152 143 112 108
82 39
AseI AT'TA_AT
Cuts at: 160 1032 5339 7246 160
Size: 872 4307 1907 1606
Fragments arranged by size:
4307 1907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8692
AvaI C'yCGr_G
Cuts at: 1870 1943 5237 6017 1870
Size: 73 3294 780 4545
Fragments arranged by size:
4545 3294 780 73
BamHI G'GATC_C
Cuts at: 2399 6022 6389 2399
Size: 3623 367 4702
Fragments arranged by size:
4702 3623 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6013 7468 618
Size: 325 217 627 4226 1455 1842
Fragments arranged by size:
4226 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5315 5764 6011 1391
Size: 3924 449 247 4072
Fragments arranged by size:
4072 3924 449 247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2480 2805 4332 5678 928
Size: 971 581 325 1527 1346 3942
Fragments arranged by size:
3942 1527 1346 971 581 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6883 6917 1903
Size: 34 4946 34 3678
Fragments arranged by size:
4946 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6584 7794 6584
Size: 1210 7482
BclI T'GATC_A
Cuts at: 1548 3214 5647 1548
Size: 1666 2433 4593
Fragments arranged by size:
4593 2433 1666
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7303 136
Size: 107 122 71 631 605 5631 1525
Fragments arranged by size:
5631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5305 8687 5305
Size: 3382 5310
BlpI GC'TnA_GC
Cuts at: 6109 6109
Size: 8692
Bme1580I G_kGCm'C
Cuts at: 1790 5315 6753 7909 1790
Size: 3525 1438 1156 2573
Fragments arranged by size:
3525 2573 1438 1156
BmgBI CAC'GTC
Cuts at: 1318 2125 1318
Size: 807 7885
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2868 4051 6423
Size: 416 53 83 399 24 1451 1183 2372
Cuts at: 6423 7381 442
Size: 958 1753
Fragments arranged by size:
2372 1753 1451 1183 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3174 4364 4371 4980 5805 7352 1239
Size: 1935 1190 7 609 825 1547 2579
Fragments arranged by size:
2579 1935 1547 1190 825 609 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3009 4706 4788 6817 7682 7980 3009
Size: 1697 82 2029 865 298 3721
Fragments arranged by size:
3721 2029 1697 865 298 82
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3894 7355 882
Size: 617 2395 3461 2219
Fragments arranged by size:
3461 2395 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5989 6483 493
Size: 711 4785 494 2702
Fragments arranged by size:
4785 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8686 8686
Size: 8692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6499 6881 7516 275
Size: 53 83 186 936 4966 382 635 1451
Fragments arranged by size:
4966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4086 4677 7125 7523 7797 7944 8355 4086
Size: 591 2448 398 274 147 411 4423
Fragments arranged by size:
4423 2448 591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4059 4089 4218 4248 5064
Size: 30 165 30 2656 30 129 30 816
Cuts at: 5064 5094 1178
Size: 30 4776
Fragments arranged by size:
4776 2656 816 165 129 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 2714 4470 4836 1186
Size: 894 634 1756 366 5042
Fragments arranged by size:
5042 1756 894 634 366
BseYI C'CCAG_C
Cuts at: 2118 2668 3571 4823 5623 5712 7895 8391 2118
Size: 550 903 1252 800 89 2183 496 2419
Fragments arranged by size:
2419 2183 1252 903 800 550 496 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2909 4022 4392 1163
Size: 573 1173 1113 370 5463
Fragments arranged by size:
5463 1173 1113 573 370
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6142 6903 7931 1540
Size: 275 186 4141 761 1028 2301
Fragments arranged by size:
4141 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 4380 5539 5764 6753 6838 7909 2031
Size: 2349 1159 225 989 85 1071 2814
Fragments arranged by size:
2814 2349 1159 1071 989 225 85
BsmI GAATG_Cn'
Cuts at: 3544 4827 6203 6296 3544
Size: 1283 1376 93 5940
Fragments arranged by size:
5940 1376 1283 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 4380 5315 5539 5764 6011 6753
Size: 399 241 2349 935 224 225 247 742
Cuts at: 6753 6838 7909 1391
Size: 85 1071 2174
Fragments arranged by size:
2349 2174 1071 935 742 399 247 241
225 224 85
BspEI T'CCGG_A
Cuts at: 4677 8355 4677
Size: 3678 5014
BspHI T'CATG_A
Cuts at: 5644 6581 8311 5644
Size: 937 1730 6025
Fragments arranged by size:
6025 1730 937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3383 4044 6047 844
Size: 2539 661 2003 3489
Fragments arranged by size:
3489 2539 2003 661
BsrBI CCG'CTC
Cuts at: 1394 1823 6579 1394
Size: 429 4756 3507
Fragments arranged by size:
4756 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7187 7361 65
Size: 1183 5939 174 1396
Fragments arranged by size:
5939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2050 4442 7336 7523 1700
Size: 110 240 2392 2894 187 2869
Fragments arranged by size:
2894 2869 2392 240 187 110
BsrGI T'GTAC_A
Cuts at: 96 2347 2752 5722 96
Size: 2251 405 2970 3066
Fragments arranged by size:
3066 2970 2251 405
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8692
BssSI C'ACGA_G
Cuts at: 6752 7764 6752
Size: 1012 7680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8692
BstBI TT'CG_AA
Cuts at: 2007 7496 2007
Size: 5489 3203
BstEII G'GTnAC_C
Cuts at: 1184 3831 1184
Size: 2647 6045
BstXI CCAn_nnnn'nTGG
Cuts at: 4831 4831
Size: 8692
BstYI r'GATC_y
Cuts at: 1972 2200 2399 5305 6022 6389 6774 6791 8243
Size: 228 199 2906 717 367 385 17 1452
Cuts at: 8243 8329 8341 8687 1972
Size: 86 12 346 1977
Fragments arranged by size:
2906 1977 1452 717 385 367 346 228
199 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3264 3264
Size: 8692
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 7183
Fragments arranged by size:
7183 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2115 487
Size: 683 56 889 7064
Fragments arranged by size:
7064 889 683 56
BtsI GCAGTG_nn'
Cuts at: 4380 5231 5836 5937 6208 7002 7022 4380
Size: 851 605 101 271 794 20 6050
Fragments arranged by size:
6050 851 794 605 271 101 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3231 3266 529
Size: 35 2667 35 5955
Fragments arranged by size:
5955 2667 35 35
DraI TTT'AAA
Cuts at: 2574 3474 4560 5996 2574
Size: 900 1086 1436 5270
Fragments arranged by size:
5270 1436 1086 900
DraIII CAC_nnn'GTG
Cuts at: 4045 4045
Size: 8692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7699 809
Size: 507 6383 1802
Fragments arranged by size:
6383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2048 3888 4494
Size: 54 1425 50 275 18 218 1840 606
Cuts at: 4494 5165 5220 5300 6139 7028 8657 8
Size: 671 55 80 839 889 1629 43
Fragments arranged by size:
1840 1629 1425 889 839 671 606 275
218 80 55 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6139 1537
Size: 275 4327 4090
Fragments arranged by size:
4327 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 4088 4148 5598 6622 1353
Size: 1335 1400 60 1450 1024 3423
Fragments arranged by size:
3423 1450 1400 1335 1024 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7279 7653 7799 387
Size: 1064 138 5690 374 146 1280
Fragments arranged by size:
5690 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5762 5762
Size: 8692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 3105 3174 3429 3468 3724 4364
Size: 393 593 880 69 255 39 256 640
Cuts at: 4364 4371 4980 4990 5370 5805 5826 6755 7352
Size: 7 609 10 380 435 21 929 597
Cuts at: 7352 8139 8407 1239
Size: 787 268 1524
Fragments arranged by size:
1524 929 880 787 640 609 597 593
435 393 380 268 256 255 69 39
21 10 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3263 3263
Size: 8692
EcoO109I rG'GnC_Cy
Cuts at: 2959 5312 2959
Size: 2353 6339
EcoRI G'AATT_C
Cuts at: 4690 5424 5858 6001 6379 4690
Size: 734 434 143 378 7003
Fragments arranged by size:
7003 734 434 378 143
EcoRV GAT'ATC
Cuts at: 5867 5867
Size: 8692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3289 3901 5477 3289
Size: 612 1576 6504
Fragments arranged by size:
6504 1576 612
FspI TGC'GCA
Cuts at: 7198 7198
Size: 8692
HaeII r_GCGC'y
Cuts at: 1678 7839 1678
Size: 6161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2216 2248 4800 4832 7333
Size: 32 135 32 644 32 2552 32 2501
Cuts at: 7333 7365 7407 7439 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2552 2501 644 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2692 3945 4519 6036 6282 669
Size: 988 281 754 1253 574 1517 246 3079
Fragments arranged by size:
3079 1517 1253 988 754 574 281 246
HindIII A'AGCT_T
Cuts at: 748 2535 748
Size: 1787 6905
HpaI GTT'AAC
Cuts at: 6282 6282
Size: 8692
KpnI G_GTAC'C
Cuts at: 6017 6017
Size: 8692
MfeI C'AATT_G
Cuts at: 3819 6291 3819
Size: 2472 6220
MluI A'CGCG_T
Cuts at: 8568 8568
Size: 8692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2361 4204 4380 7806 7990 1305
Size: 1056 1843 176 3426 184 2007
Fragments arranged by size:
3426 2007 1843 1056 184 176
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3890 5167 5222 10
Size: 54 1425 343 2058 1277 55 3480
Fragments arranged by size:
3480 2058 1425 1277 343 55 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2556 5385 5550 5798 6650 7009 7168
Size: 648 1390 2829 165 248 852 359 159
Cuts at: 7168 518
Size: 2042
Fragments arranged by size:
2829 2042 1390 852 648 359 248 165
159
MspA1I CmG'CkG
Cuts at: 1606 2118 3189 4412 4509 6108 6785 7933 8178
Size: 512 1071 1223 97 1599 677 1148 245
Cuts at: 8178 1606
Size: 2120
Fragments arranged by size:
2120 1599 1223 1148 1071 677 512 245
97
NaeI GCC'GGC
Cuts at: 4444 4444
Size: 8692
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 7183
Fragments arranged by size:
7183 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8692
NgoMIV G'CCGG_C
Cuts at: 4442 4442
Size: 8692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8692
NotI GC'GGCC_GC
Cuts at: 6139 6139
Size: 8692
NspI r_CATG'y
Cuts at: 1132 1229 2775 3350 5831 6050 7595 1132
Size: 97 1546 575 2481 219 1545 2229
Fragments arranged by size:
2481 2229 1546 1545 575 219 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8692
PciI A'CATG_T
Cuts at: 2771 7591 2771
Size: 4820 3872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8692
PfoI T'CCnGG_A
Cuts at: 2156 3548 4201 2156
Size: 1392 653 6647
Fragments arranged by size:
6647 1392 653
PmeI GTTT'AAAC
Cuts at: 5996 5996
Size: 8692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4218 4250 6761 6793 8274 8306 4218
Size: 32 2511 32 1481 32 4604
Fragments arranged by size:
4604 2511 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2959 2959
Size: 8692
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8692
PsiI TTA'TAA
Cuts at: 6262 6262
Size: 8692
PspOMI G'GGCC_C
Cuts at: 5311 5311
Size: 8692
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2331 2363 2331
Size: 32 8660
PstI C_TGCA'G
Cuts at: 830 2941 3333 6044 830
Size: 2111 392 2711 3478
Fragments arranged by size:
3478 2711 2111 392
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8692
PvuII CAG'CTG
Cuts at: 2118 3189 4412 2118
Size: 1071 1223 6398
Fragments arranged by size:
6398 1223 1071
SacI G_AGCT'C
Cuts at: 5764 5764
Size: 8692
SalI G'TCGA_C
Cuts at: 1936 6034 1936
Size: 4098 4594
SapI GCTCTTCn'nnn_
Cuts at: 5598 5598
Size: 8692
SbfI CC_TGCA'GG
Cuts at: 6044 6044
Size: 8692
ScaI AGT'ACT
Cuts at: 2977 6940 8652 2977
Size: 3963 1712 3017
Fragments arranged by size:
3963 3017 1712
SfcI C'TryA_G
Cuts at: 826 1045 2263 2937 3329 4698 6040 7175 7856
Size: 219 1218 674 392 1369 1342 1135 681
Cuts at: 7856 8047 826
Size: 191 1471
Fragments arranged by size:
1471 1369 1342 1218 1135 681 674 392
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8692
SmaI CCC'GGG
Cuts at: 1872 6019 1872
Size: 4147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3024 4721 4803 5156 5462 6796
Size: 197 926 1081 1697 82 353 306 1334
Cuts at: 6796 7697 7959 820
Size: 901 262 1553
Fragments arranged by size:
1697 1553 1334 1081 926 901 353 306
262 197 82
SnaBI TAC'GTA
Cuts at: 493 5989 6483 493
Size: 5496 494 2702
Fragments arranged by size:
5496 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8692
SphI G_CATG'C
Cuts at: 1132 6050 1132
Size: 4918 3774
SspI AAT'ATT
Cuts at: 5 52 6616 5
Size: 47 6564 2081
Fragments arranged by size:
6564 2081 47
StuI AGG'CCT
Cuts at: 1517 2867 3608 4801 1517
Size: 1350 741 1193 5408
Fragments arranged by size:
5408 1350 1193 741
StyI C'CwwG_G
Cuts at: 513 1065 2022 3007 6131 513
Size: 552 957 985 3124 3074
Fragments arranged by size:
3124 3074 985 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4033 4406 5682 6719 6736 6889 7074
Size: 1255 2453 373 1276 1037 17 153 185
Cuts at: 7074 7519 325
Size: 445 1498
Fragments arranged by size:
2453 1498 1276 1255 1037 445 373 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2347 2752 2933
Size: 274 80 33 51 1443 370 405 181
Cuts at: 2933 2975 3191 3879 5241 5722 6938 8650 96
Size: 42 216 688 1362 481 1216 1712 138
Fragments arranged by size:
1712 1443 1362 1216 688 481 405 370
274 216 181 138 80 51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4059 4091 4722 4754 8652 8684 4059
Size: 32 631 32 3898 32 4067
Fragments arranged by size:
4067 3898 631 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8692
XbaI T'CTAG_A
Cuts at: 6028 6028
Size: 8692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2634 3850 5538 2634
Size: 1216 1688 5788
Fragments arranged by size:
5788 1688 1216
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8692
XmaI C'CCGG_G
Cuts at: 1870 6017 1870
Size: 4147 4545
XmnI GAAnn'nnTTC
Cuts at: 5109 6821 5109
Size: 1712 6980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6500 276
Size: 53 83 186 936 4966 2468
Fragments arranged by size:
4966 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI