Restriction map for
pFN21ASDA0741
(Circular) (Six-base) MAPSORT of: pFN21ASDA0741.seq Check: 7865 from: 1 to: 8389
pFN21ASDA0741 8389 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3995 3995
Size: 8389
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6199 278
Size: 53 83 186 936 4663 2468
Fragments arranged by size:
4663 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5732 1937
Size: 3795 4594
Acc65I G'GTAC_C
Cuts at: 4483 5710 4483
Size: 1227 7162
AclI AA'CG_TT
Cuts at: 1563 6516 6889 1563
Size: 4953 373 3063
Fragments arranged by size:
4953 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2928 3636 5070 5681 6452 7836 8104 1632
Size: 1296 708 1434 611 771 1384 268 1917
Fragments arranged by size:
1917 1434 1384 1296 771 708 611 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8192
AflIII A'CryG_T
Cuts at: 1203 3985 7288 8265 1203
Size: 2782 3303 977 1327
Fragments arranged by size:
3303 2782 1327 977
AgeI A'CCGG_T
Cuts at: 7220 7220
Size: 8389
AhdI GACnn_n'nnGTC
Cuts at: 7118 7118
Size: 8389
AleI CACnn'nnGTG
Cuts at: 1166 4472 5395 1166
Size: 3306 923 4160
Fragments arranged by size:
4160 3306 923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8389
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3573 3605 3573
Size: 32 8357
AlwNI CAG_nnn'CTG
Cuts at: 3794 6132 7704 3794
Size: 2338 1572 4479
Fragments arranged by size:
4479 2338 1572
ApaI G_GGCC'C
Cuts at: 4710 4710
Size: 8389
ApaLI G'TGCA_C
Cuts at: 6446 7602 6446
Size: 1156 7233
ApoI r'AATT_y
Cuts at: 1493 2291 2563 2597 2846 3960 4081 4379 4412
Size: 798 272 34 249 1114 121 298 33
Cuts at: 4412 5009 5187 5270 5413 5698 5924 6076 8185
Size: 597 178 83 143 285 226 152 2109
Cuts at: 8185 1493
Size: 1697
Fragments arranged by size:
2109 1697 1114 798 597 298 285 272
249 226 178 152 143 121 83 34
33
AseI AT'TA_AT
Cuts at: 160 1032 4056 6943 160
Size: 872 3024 2887 1606
Fragments arranged by size:
3024 2887 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8389
AvaI C'yCGr_G
Cuts at: 1870 1943 4621 5334 5714 1870
Size: 73 2678 713 380 4545
Fragments arranged by size:
4545 2678 713 380 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2877 2910 2877
Size: 33 8356
BamHI G'GATC_C
Cuts at: 5719 6086 5719
Size: 367 8022
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4483 4631 5710 7165 618
Size: 325 217 627 2696 148 1079 1455 1842
Fragments arranged by size:
2696 1842 1455 1079 627 325 217 148
BanII G_rGCy'C
Cuts at: 1391 3788 3916 4227 4710 5708 1391
Size: 2397 128 311 483 998 4072
Fragments arranged by size:
4072 2397 998 483 311 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 3758 3959 5548 928
Size: 971 159 1700 201 1589 3769
Fragments arranged by size:
3769 1700 1589 971 201 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6580 6614 1903
Size: 34 4643 34 3678
Fragments arranged by size:
4643 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6281 7491 6281
Size: 1210 7179
BclI T'GATC_A
Cuts at: 1548 4653 1548
Size: 3105 5284
BfrBI ATG'CAT
Cuts at: 4213 4213
Size: 8389
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7000 136
Size: 107 122 71 631 605 5328 1525
Fragments arranged by size:
5328 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8384 8384
Size: 8389
BlpI GC'TnA_GC
Cuts at: 3069 5806 3069
Size: 2737 5652
Bme1580I G_kGCm'C
Cuts at: 1790 4634 4710 5034 6450 7606 1790
Size: 2844 76 324 1416 1156 2573
Fragments arranged by size:
2844 2573 1416 1156 324 76
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8389
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4994 5051 6120
Size: 416 53 83 399 24 3577 57 1069
Cuts at: 6120 7078 442
Size: 958 1753
Fragments arranged by size:
3577 1753 1069 958 416 399 83 57
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2910 4716 4856 7049 1239
Size: 1671 1806 140 2193 2579
Fragments arranged by size:
2579 2193 1806 1671 140
Bpu10I CC'TnA_GC
Cuts at: 3306 4806 3306
Size: 1500 6889
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2214 6514 7379 7677 2214
Size: 4300 865 298 2926
Fragments arranged by size:
4300 2926 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7052 882
Size: 617 5553 2219
Fragments arranged by size:
5553 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5686 6180 493
Size: 711 4482 494 2702
Fragments arranged by size:
4482 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3813 5306 8383 3813
Size: 1493 3077 3819
Fragments arranged by size:
3819 3077 1493
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2682 6196 6578 7213
Size: 53 83 186 936 1149 3514 382 635
Cuts at: 7213 275
Size: 1451
Fragments arranged by size:
3514 1451 1149 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6822 7220 7494 7641 8052 6822
Size: 398 274 147 411 7159
Fragments arranged by size:
7159 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3469 3499 4953 4983 1178
Size: 30 165 30 2066 30 1454 30 4584
Fragments arranged by size:
4584 2066 1454 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2531 3575 3635 3638 3641 3644 3686 3695
Size: 1345 1044 60 3 3 3 42 9
Cuts at: 3695 4400 4734 1186
Size: 705 334 4841
Fragments arranged by size:
4841 1345 1044 705 334 60 42 9
3 3 3
BseYI C'CCAG_C
Cuts at: 7592 8088 7592
Size: 496 7893
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4479 5217 1163
Size: 573 2743 738 4335
Fragments arranged by size:
4335 2743 738 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5839 6600 7628 1540
Size: 275 186 3838 761 1028 2301
Fragments arranged by size:
3838 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3788 4597 6450 6535 7606 3788
Size: 809 1853 85 1071 4571
Fragments arranged by size:
4571 1853 1071 809 85
BsmI GAATG_Cn'
Cuts at: 4119 4438 5900 5993 4119
Size: 319 1462 93 6515
Fragments arranged by size:
6515 1462 319 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3788 3916 4227 4597 4634 4710 5034
Size: 399 1998 128 311 370 37 76 324
Cuts at: 5034 5708 6450 6535 7606 1391
Size: 674 742 85 1071 2174
Fragments arranged by size:
2174 1998 1071 742 674 399 370 324
311 128 85 76 37
BspEI T'CCGG_A
Cuts at: 8052 8052
Size: 8389
BspHI T'CATG_A
Cuts at: 6278 8008 6278
Size: 1730 6659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3995 5360 5744 844
Size: 3151 1365 384 3489
Fragments arranged by size:
3489 3151 1365 384
BsrBI CCG'CTC
Cuts at: 1394 1823 6276 1394
Size: 429 4453 3507
Fragments arranged by size:
4453 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4185 6884 7058 65
Size: 1183 2937 2699 174 1396
Fragments arranged by size:
2937 2699 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5583 7033 7220 1700
Size: 110 3773 1450 187 2869
Fragments arranged by size:
3773 2869 1450 187 110
BsrGI T'GTAC_A
Cuts at: 96 3988 96
Size: 3892 4497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8389
BssSI C'ACGA_G
Cuts at: 6449 7461 6449
Size: 1012 7377
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8389
BstBI TT'CG_AA
Cuts at: 2007 7193 2007
Size: 5186 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8389
BstYI r'GATC_y
Cuts at: 1972 4459 5338 5719 6086 6471 6488 7940 8026
Size: 2487 879 381 367 385 17 1452 86
Cuts at: 8026 8038 8384 1972
Size: 12 346 1977
Fragments arranged by size:
2487 1977 1452 879 385 381 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6880
Fragments arranged by size:
6880 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7650
Fragments arranged by size:
7650 683 56
BtsI GCAGTG_nn'
Cuts at: 2268 3706 5905 6699 6719 2268
Size: 1438 2199 794 20 3938
Fragments arranged by size:
3938 2199 1438 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8354
DraI TTT'AAA
Cuts at: 4350 5693 4350
Size: 1343 7046
DraIII CAC_nnn'GTG
Cuts at: 4580 4580
Size: 8389
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4237 7396 809
Size: 507 2921 3159 1802
Fragments arranged by size:
3159 2921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3549 5836 6725
Size: 54 1425 50 275 18 1719 2287 889
Cuts at: 6725 8354 8
Size: 1629 43
Fragments arranged by size:
2287 1719 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5836 1537
Size: 275 4024 4090
Fragments arranged by size:
4090 4024 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2280 2787 2904 3092 3099 3150 3341 3468
Size: 927 507 117 188 7 51 191 127
Cuts at: 3468 3612 3633 3663 3672 4578 5627 6319 1353
Size: 144 21 30 9 906 1049 692 3423
Fragments arranged by size:
3423 1049 927 906 692 507 191 188
144 127 117 51 30 21 9 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6976 7350 7496 387
Size: 1064 138 5387 374 146 1280
Fragments arranged by size:
5387 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3786 3786
Size: 8389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2910 2928 3636 4716 4856 5070 5681
Size: 393 1278 18 708 1080 140 214 611
Cuts at: 5681 6452 7049 7836 8104 1239
Size: 771 597 787 268 1524
Fragments arranged by size:
1524 1278 1080 787 771 708 611 597
393 268 214 140 18
EcoO109I rG'GnC_Cy
Cuts at: 2648 3026 4706 2648
Size: 378 1680 6331
Fragments arranged by size:
6331 1680 378
EcoRI G'AATT_C
Cuts at: 2291 5698 6076 2291
Size: 3407 378 4604
Fragments arranged by size:
4604 3407 378
EcoRV GAT'ATC
Cuts at: 2194 2194
Size: 8389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 3257 3305 2194
Size: 1063 48 7278
Fragments arranged by size:
7278 1063 48
FspI TGC'GCA
Cuts at: 6895 6895
Size: 8389
HaeII r_GCGC'y
Cuts at: 1678 7536 1678
Size: 5858 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2372 2404 2669 2701 3469
Size: 32 135 32 800 32 265 32 768
Cuts at: 3469 3501 4668 4700 4953 4985 7030 7062 7104
Size: 32 1167 32 253 32 2045 32 42
Cuts at: 7104 7136 1373
Size: 32 2626
Fragments arranged by size:
2626 2045 1167 800 768 265 253 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4545 5422 5733 5979 669
Size: 988 281 2607 877 311 246 3079
Fragments arranged by size:
3079 2607 988 877 311 281 246
HindIII A'AGCT_T
Cuts at: 748 3071 748
Size: 2323 6066
HpaI GTT'AAC
Cuts at: 4545 5979 4545
Size: 1434 6955
KpnI G_GTAC'C
Cuts at: 4487 5714 4487
Size: 1227 7162
MfeI C'AATT_G
Cuts at: 4233 5988 4233
Size: 1755 6634
MluI A'CGCG_T
Cuts at: 8265 8265
Size: 8389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5076 7503 7687 1305
Size: 3771 2427 184 2007
Fragments arranged by size:
3771 2427 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3551 10
Size: 54 1425 343 1719 4848
Fragments arranged by size:
4848 1719 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3984 4472 4503 5395 6347 6706 6865
Size: 648 2818 488 31 892 952 359 159
Cuts at: 6865 518
Size: 2042
Fragments arranged by size:
2818 2042 952 892 648 488 359 159
31
MspA1I CmG'CkG
Cuts at: 1606 2933 5805 6482 7630 7875 1606
Size: 1327 2872 677 1148 245 2120
Fragments arranged by size:
2872 2120 1327 1148 677 245
NaeI GCC'GGC
Cuts at: 5585 5585
Size: 8389
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6880
Fragments arranged by size:
6880 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8389
NgoMIV G'CCGG_C
Cuts at: 5583 5583
Size: 8389
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8389
NotI GC'GGCC_GC
Cuts at: 5836 5836
Size: 8389
NsiI A_TGCA'T
Cuts at: 4215 4215
Size: 8389
NspI r_CATG'y
Cuts at: 1132 1229 3941 3989 5747 7292 1132
Size: 97 2712 48 1758 1545 2229
Fragments arranged by size:
2712 2229 1758 1545 97 48
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8389
PciI A'CATG_T
Cuts at: 3985 7288 3985
Size: 3303 5086
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8389
PfoI T'CCnGG_A
Cuts at: 4108 4693 4108
Size: 585 7804
PmeI GTTT'AAAC
Cuts at: 5693 5693
Size: 8389
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6458 6490 7971 8003 6458
Size: 32 1481 32 6844
Fragments arranged by size:
6844 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2648 3026 2648
Size: 378 8011
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8389
PsiI TTA'TAA
Cuts at: 4875 5959 4875
Size: 1084 7305
PspOMI G'GGCC_C
Cuts at: 4706 4706
Size: 8389
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3444 3476 3444
Size: 32 8357
PstI C_TGCA'G
Cuts at: 830 2095 2938 5741 830
Size: 1265 843 2803 3478
Fragments arranged by size:
3478 2803 1265 843
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8389
PvuII CAG'CTG
Cuts at: 2933 2933
Size: 8389
SacI G_AGCT'C
Cuts at: 3788 3788
Size: 8389
SalI G'TCGA_C
Cuts at: 1936 5731 1936
Size: 3795 4594
SapI GCTCTTCn'nnn_
Cuts at: 4578 4578
Size: 8389
SbfI CC_TGCA'GG
Cuts at: 5741 5741
Size: 8389
ScaI AGT'ACT
Cuts at: 6637 8349 6637
Size: 1712 6677
SfcI C'TryA_G
Cuts at: 826 1045 2091 2934 3438 4638 5556 5737 6872
Size: 219 1046 843 504 1200 918 181 1135
Cuts at: 6872 7553 7744 826
Size: 681 191 1471
Fragments arranged by size:
1471 1200 1135 1046 918 843 681 504
219 191 181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8389
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8389
SmaI CCC'GGG
Cuts at: 1872 4623 5716 1872
Size: 2751 1093 4545
Fragments arranged by size:
4545 2751 1093
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2229 5334 6493 7394 7656 820
Size: 197 926 286 3105 1159 901 262 1553
Fragments arranged by size:
3105 1553 1159 926 901 286 262 197
SnaBI TAC'GTA
Cuts at: 493 5686 6180 493
Size: 5193 494 2702
Fragments arranged by size:
5193 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8389
SphI G_CATG'C
Cuts at: 1132 5747 1132
Size: 4615 3774
SspI AAT'ATT
Cuts at: 5 52 6313 5
Size: 47 6261 2081
Fragments arranged by size:
6261 2081 47
StuI AGG'CCT
Cuts at: 1517 4731 1517
Size: 3214 5175
StyI C'CwwG_G
Cuts at: 513 1065 2022 2061 2085 5828 513
Size: 552 957 39 24 3743 3074
Fragments arranged by size:
3743 3074 957 552 39 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5407 5542 6416 6433 6586 6771 7216
Size: 1255 3827 135 874 17 153 185 445
Cuts at: 7216 325
Size: 1498
Fragments arranged by size:
3827 1498 1255 874 445 185 153 135
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 3988 5319
Size: 274 80 33 51 1443 444 1567 1331
Cuts at: 5319 6635 8347 96
Size: 1316 1712 138
Fragments arranged by size:
1712 1567 1443 1331 1316 444 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4024 4056 8349 8381 4024
Size: 32 4293 32 4032
Fragments arranged by size:
4293 4032 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8389
XbaI T'CTAG_A
Cuts at: 2609 3964 5725 2609
Size: 1355 1761 5273
Fragments arranged by size:
5273 1761 1355
XhoI C'TCGA_G
Cuts at: 1943 5334 1943
Size: 3391 4998
XmaI C'CCGG_G
Cuts at: 1870 4621 5714 1870
Size: 2751 1093 4545
Fragments arranged by size:
4545 2751 1093
XmnI GAAnn'nnTTC
Cuts at: 2563 6518 2563
Size: 3955 4434
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6197 276
Size: 53 83 186 936 4663 2468
Fragments arranged by size:
4663 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NarI
NruI PacI PmlI PspXI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI