Restriction map for
pFN21ASDA0738
(Circular) (Six-base) MAPSORT of: pFN21ASDA0738.seq Check: 6029 from: 1 to: 7492
pFN21ASDA0738 7492 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4364 4364
Size: 7492
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5302 278
Size: 53 83 186 936 3766 2468
Fragments arranged by size:
3766 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3021 4835 1937
Size: 1084 1814 4594
Fragments arranged by size:
4594 1814 1084
Acc65I G'GTAC_C
Cuts at: 4206 4813 4206
Size: 607 6885
AclI AA'CG_TT
Cuts at: 1563 5619 5992 1563
Size: 4056 373 3063
Fragments arranged by size:
4056 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3729 3752 5555 6939 7207 1632
Size: 2097 23 1803 1384 268 1917
Fragments arranged by size:
2097 1917 1803 1384 268 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7295
AflIII A'CryG_T
Cuts at: 1203 3396 6391 7368 1203
Size: 2193 2995 977 1327
Fragments arranged by size:
2995 2193 1327 977
AgeI A'CCGG_T
Cuts at: 3807 6323 3807
Size: 2516 4976
AhdI GACnn_n'nnGTC
Cuts at: 6221 6221
Size: 7492
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7492
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7492
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4313 4345 4313
Size: 32 7460
AlwNI CAG_nnn'CTG
Cuts at: 5235 6807 5235
Size: 1572 5920
ApaI G_GGCC'C
Cuts at: 2873 3225 4002 4286 2873
Size: 352 777 284 6079
Fragments arranged by size:
6079 777 352 284
ApaLI G'TGCA_C
Cuts at: 5549 6705 5549
Size: 1156 6336
ApoI r'AATT_y
Cuts at: 1493 4801 5027 5179 7288 1493
Size: 3308 226 152 2109 1697
Fragments arranged by size:
3308 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6046 160
Size: 872 5014 1606
Fragments arranged by size:
5014 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
AvaI C'yCGr_G
Cuts at: 1870 1943 2336 3456 4817 1870
Size: 73 393 1120 1361 4545
Fragments arranged by size:
4545 1361 1120 393 73
BamHI G'GATC_C
Cuts at: 4822 5189 4822
Size: 367 7125
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2317 3638 4206 4813 6268
Size: 325 217 627 530 1321 568 607 1455
Cuts at: 6268 618
Size: 1842
Fragments arranged by size:
1842 1455 1321 627 607 568 530 325
217
BanII G_rGCy'C
Cuts at: 1391 2293 2345 2873 2997 3106 3225 3669 4002
Size: 902 52 528 124 109 119 444 333
Cuts at: 4002 4137 4245 4286 4811 1391
Size: 135 108 41 525 4072
Fragments arranged by size:
4072 902 528 525 444 333 135 124
119 109 108 52 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6521
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5683 5717 1903
Size: 34 3746 34 3678
Fragments arranged by size:
3746 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5384 6594 3992
Size: 1392 1210 4890
Fragments arranged by size:
4890 1392 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7492
BfrBI ATG'CAT
Cuts at: 4198 4499 4198
Size: 301 7191
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 2916 6103
Size: 107 122 71 631 605 376 868 3187
Cuts at: 6103 136
Size: 1525
Fragments arranged by size:
3187 1525 868 631 605 376 122 107
71
BglII A'GATC_T
Cuts at: 4179 7487 4179
Size: 3308 4184
BlpI GC'TnA_GC
Cuts at: 4131 4909 4131
Size: 778 6714
Bme1580I G_kGCm'C
Cuts at: 1790 2873 3225 4002 4286 5553 6709 1790
Size: 1083 352 777 284 1267 1156 2573
Fragments arranged by size:
2573 1267 1156 1083 777 352 284
BmgBI CAC'GTC
Cuts at: 1318 4174 1318
Size: 2856 4636
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2624 2876 3672
Size: 416 53 83 399 24 1207 252 796
Cuts at: 3672 3902 4133 4430 5223 6181 442
Size: 230 231 297 793 958 1753
Fragments arranged by size:
1753 1207 958 796 793 416 399 297
252 231 230 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7492
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3812
Size: 7492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2367 3983 4085 4247 4514 6152 1239
Size: 1128 1616 102 162 267 1638 2579
Fragments arranged by size:
2579 1638 1616 1128 267 162 102
Bpu10I CC'TnA_GC
Cuts at: 4074 4466 4074
Size: 392 7100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5617 6482 6780 5617
Size: 865 298 6329
Fragments arranged by size:
6329 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3947 6155 882
Size: 617 2448 2208 2219
Fragments arranged by size:
2448 2219 2208 617
BsaAI yAC'GTr
Cuts at: 493 1204 3843 4789 5283 493
Size: 711 2639 946 494 2702
Fragments arranged by size:
2702 2639 946 711 494
BsaBI GATnn'nnATC
Cuts at: 7486 7486
Size: 7492
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5299 5681 6316 275
Size: 53 83 186 936 3766 382 635 1451
Fragments arranged by size:
3766 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3807 5925 6323 6597 6744 7155 3807
Size: 2118 398 274 147 411 4144
Fragments arranged by size:
4144 2118 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3251 3281 4313 4343 1178
Size: 30 165 30 1848 30 1032 30 4327
Fragments arranged by size:
4327 1848 1032 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2167 2951 3161 3164 3851 3980 4757 1186
Size: 981 784 210 3 687 129 777 3921
Fragments arranged by size:
3921 981 784 777 687 210 129 3
BseYI C'CCAG_C
Cuts at: 2073 2235 2730 3340 3946 4297 6695 7191 2073
Size: 162 495 610 606 351 2398 496 2374
Fragments arranged by size:
2398 2374 610 606 496 495 351 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2951 3375 3610 4196 4256 4673 1163
Size: 573 1215 424 235 586 60 417 3982
Fragments arranged by size:
3982 1215 586 573 424 417 235 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4047 4942 5703 6731 1540
Size: 275 186 2046 895 761 1028 2301
Fragments arranged by size:
2301 2046 1028 895 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4245 5553 5638 6709 4245
Size: 1308 85 1071 5028
Fragments arranged by size:
5028 1308 1071 85
BsmI GAATG_Cn'
Cuts at: 4398 4501 5003 5096 4398
Size: 103 502 93 6794
Fragments arranged by size:
6794 502 103 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4379 4568 4379
Size: 189 7303
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2293 2345 2873 2997 3106 3225 3669
Size: 399 503 52 528 124 109 119 444
Cuts at: 3669 4002 4137 4245 4286 4811 5553 5638 6709
Size: 333 135 108 41 525 742 85 1071
Cuts at: 6709 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 528 525 503 444 399
333 135 124 119 109 108 85 52
41
BspEI T'CCGG_A
Cuts at: 7155 7155
Size: 7492
BspHI T'CATG_A
Cuts at: 2445 5381 7111 2445
Size: 2936 1730 2826
Fragments arranged by size:
2936 2826 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4364 4847 844
Size: 3520 483 3489
Fragments arranged by size:
3520 3489 483
BsrBI CCG'CTC
Cuts at: 1394 1823 3267 5379 1394
Size: 429 1444 2112 3507
Fragments arranged by size:
3507 2112 1444 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2166 5987 6161 65
Size: 1183 918 3821 174 1396
Fragments arranged by size:
3821 1396 1183 918 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3807 6136 6323 1700
Size: 110 1997 2329 187 2869
Fragments arranged by size:
2869 2329 1997 187 110
BsrGI T'GTAC_A
Cuts at: 96 2302 3570 96
Size: 2206 1268 4018
Fragments arranged by size:
4018 2206 1268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7492
BssSI C'ACGA_G
Cuts at: 2543 2590 5552 6564 2543
Size: 47 2962 1012 3471
Fragments arranged by size:
3471 2962 1012 47
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7492
BstBI TT'CG_AA
Cuts at: 2007 6296 2007
Size: 4289 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7492
BstXI CCAn_nnnn'nTGG
Cuts at: 3517 4002 3517
Size: 485 7007
BstYI r'GATC_y
Cuts at: 1972 2625 3384 4179 4822 5189 5574 5591 7043
Size: 653 759 795 643 367 385 17 1452
Cuts at: 7043 7129 7141 7487 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 795 759 653 643 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3416 3416
Size: 7492
BtgI C'CryG_G
Cuts at: 513 1065 2022 2188 2782 4017 513
Size: 552 957 166 594 1235 3988
Fragments arranged by size:
3988 1235 957 594 552 166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6753
Fragments arranged by size:
6753 683 56
BtsI GCAGTG_nn'
Cuts at: 2268 2973 3701 5008 5802 5822 2268
Size: 705 728 1307 794 20 3938
Fragments arranged by size:
3938 1307 794 728 705 20
ClaI AT'CG_AT
Cuts at: 1554 3836 1554
Size: 2282 5210
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3095 3130 529
Size: 35 2531 35 4891
Fragments arranged by size:
4891 2531 35 35
DraI TTT'AAA
Cuts at: 2602 4796 2602
Size: 2194 5298
DraIII CAC_nnn'GTG
Cuts at: 4651 4651
Size: 7492
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6499 809
Size: 507 5183 1802
Fragments arranged by size:
5183 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2185 2191 2325
Size: 54 1425 50 275 18 355 6 134
Cuts at: 2325 2812 2836 3276 3507 4014 4939 5828 7457
Size: 487 24 440 231 507 925 889 1629
Cuts at: 7457 8
Size: 43
Fragments arranged by size:
1629 1425 925 889 507 487 440 355
275 231 134 54 50 43 24 18
6
EagI C'GGCC_G
Cuts at: 1537 1812 4939 1537
Size: 275 3127 4090
Fragments arranged by size:
4090 3127 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2284 2667 3299 3375 3968 4442 4571 5422
Size: 931 383 632 76 593 474 129 851
Cuts at: 5422 1353
Size: 3423
Fragments arranged by size:
3423 931 851 632 593 474 383 129
76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4076 6079 6453 6599 387
Size: 1064 138 2487 2003 374 146 1280
Fragments arranged by size:
2487 2003 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4243 4243
Size: 7492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2367 3729 3752 3983 4085 4247 4514
Size: 393 735 1362 23 231 102 162 267
Cuts at: 4514 5555 6152 6939 7207 1239
Size: 1041 597 787 268 1524
Fragments arranged by size:
1524 1362 1041 787 735 597 393 268
267 231 162 102 23
EcoNI CCTnn'n_nnAGG
Cuts at: 3240 4469 3240
Size: 1229 6263
EcoO109I rG'GnC_Cy
Cuts at: 2050 2161 2870 3221 3222 3252 3998 3999 4282
Size: 111 709 351 1 30 746 1 283
Cuts at: 4282 4283 4475 2050
Size: 1 192 5067
Fragments arranged by size:
5067 746 709 351 283 192 111 30
1 1 1
EcoRI G'AATT_C
Cuts at: 4801 5179 4801
Size: 378 7114
EcoRV GAT'ATC
Cuts at: 3437 3437
Size: 7492
FspI TGC'GCA
Cuts at: 5998 5998
Size: 7492
HaeII r_GCGC'y
Cuts at: 1678 6639 1678
Size: 4961 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2642 2674 4036 4068 4443
Size: 32 135 32 1070 32 1362 32 375
Cuts at: 4443 4459 4475 4491 6133 6165 6207 6239 1373
Size: 16 16 16 1642 32 42 32 2626
Fragments arranged by size:
2626 1642 1362 1070 375 135 42 32
32 32 32 32 32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3622 4612 4836 5082 669
Size: 988 281 1684 990 224 246 3079
Fragments arranged by size:
3079 1684 990 988 281 246 224
HindIII A'AGCT_T
Cuts at: 748 3779 748
Size: 3031 4461
HpaI GTT'AAC
Cuts at: 5082 5082
Size: 7492
KpnI G_GTAC'C
Cuts at: 4210 4817 4210
Size: 607 6885
MfeI C'AATT_G
Cuts at: 5091 5091
Size: 7492
MluI A'CGCG_T
Cuts at: 7368 7368
Size: 7492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2685 3079 6606 6790 1305
Size: 1380 394 3527 184 2007
Fragments arranged by size:
3527 2007 1380 394 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 2838 3509 4016 10
Size: 54 1425 343 495 511 671 507 3486
Fragments arranged by size:
3486 1425 671 511 507 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2787 3401 3756 4775 5450 5809 5968
Size: 648 1621 614 355 1019 675 359 159
Cuts at: 5968 518
Size: 2042
Fragments arranged by size:
2042 1621 1019 675 648 614 359 355
159
MspA1I CmG'CkG
Cuts at: 1606 2734 2895 4081 4315 4908 5585 6733 6978
Size: 1128 161 1186 234 593 677 1148 245
Cuts at: 6978 1606
Size: 2120
Fragments arranged by size:
2120 1186 1148 1128 677 593 245 234
161
NcoI C'CATG_G
Cuts at: 513 1065 2022 2782 513
Size: 552 957 760 5223
Fragments arranged by size:
5223 957 760 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7492
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7492
NotI GC'GGCC_GC
Cuts at: 4939 4939
Size: 7492
NsiI A_TGCA'T
Cuts at: 4200 4501 4200
Size: 301 7191
NspI r_CATG'y
Cuts at: 1132 1229 3400 3932 4202 4850 6395 1132
Size: 97 2171 532 270 648 1545 2229
Fragments arranged by size:
2229 2171 1545 648 532 270 97
PasI CC'CwG_GG
Cuts at: 1348 2285 2386 2534 4002 1348
Size: 937 101 148 1468 4838
Fragments arranged by size:
4838 1468 937 148 101
PciI A'CATG_T
Cuts at: 3396 6391 3396
Size: 2995 4497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4297 1108
Size: 3189 4303
PfoI T'CCnGG_A
Cuts at: 3984 4530 3984
Size: 546 6946
PmeI GTTT'AAAC
Cuts at: 4796 4796
Size: 7492
PmlI CAC'GTG
Cuts at: 3843 3843
Size: 7492
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 5561 5593 7074 7106 4313
Size: 32 1216 32 1481 32 4699
Fragments arranged by size:
4699 1481 1216 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2161 3252 4475 2161
Size: 1091 1223 5178
Fragments arranged by size:
5178 1223 1091
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7492
PsiI TTA'TAA
Cuts at: 3293 5062 3293
Size: 1769 5723
PspOMI G'GGCC_C
Cuts at: 2869 3221 3998 4282 2869
Size: 352 777 284 6079
Fragments arranged by size:
6079 777 352 284
PspXI vC'TCGA_Gb
Cuts at: 2336 2336
Size: 7492
PstI C_TGCA'G
Cuts at: 830 3066 3369 3488 4844 830
Size: 2236 303 119 1356 3478
Fragments arranged by size:
3478 2236 1356 303 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7492
PvuII CAG'CTG
Cuts at: 2734 2734
Size: 7492
SacI G_AGCT'C
Cuts at: 4245 4245
Size: 7492
SalI G'TCGA_C
Cuts at: 1936 4834 1936
Size: 2898 4594
SanDI GG'GwC_CC
Cuts at: 4475 4475
Size: 7492
SapI GCTCTTCn'nnn_
Cuts at: 2284 2667 2284
Size: 383 7109
SbfI CC_TGCA'GG
Cuts at: 4844 4844
Size: 7492
ScaI AGT'ACT
Cuts at: 3661 5740 7452 3661
Size: 2079 1712 3701
Fragments arranged by size:
3701 2079 1712
SfcI C'TryA_G
Cuts at: 826 1045 3062 3365 3484 4840 5975 6656 6847
Size: 219 2017 303 119 1356 1135 681 191
Cuts at: 6847 826
Size: 1471
Fragments arranged by size:
2017 1471 1356 1135 681 303 219 191
119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7492
SmaI CCC'GGG
Cuts at: 1872 4819 1872
Size: 2947 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 5596 6497 6759 820
Size: 197 926 393 3260 901 262 1553
Fragments arranged by size:
3260 1553 926 901 393 262 197
SnaBI TAC'GTA
Cuts at: 493 4789 5283 493
Size: 4296 494 2702
Fragments arranged by size:
4296 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7492
SphI G_CATG'C
Cuts at: 1132 3932 4202 4850 1132
Size: 2800 270 648 3774
Fragments arranged by size:
3774 2800 648 270
SspI AAT'ATT
Cuts at: 5 52 4420 4750 5416 5
Size: 47 4368 330 666 2081
Fragments arranged by size:
4368 2081 666 330 47
StuI AGG'CCT
Cuts at: 1517 4682 1517
Size: 3165 4327
StyI C'CwwG_G
Cuts at: 513 1065 2022 2782 3273 4269 4931 513
Size: 552 957 760 491 996 662 3074
Fragments arranged by size:
3074 996 957 760 662 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2322 5519 5536 5689 5874 6319 325
Size: 1255 742 3197 17 153 185 445 1498
Fragments arranged by size:
3197 1498 1255 742 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2302 3570 3659
Size: 274 80 33 51 1443 325 1268 89
Cuts at: 3659 4575 5738 7450 96
Size: 916 1163 1712 138
Fragments arranged by size:
1712 1443 1268 1163 916 325 274 138
89 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3251 3283 7452 7484 3251
Size: 32 4169 32 3259
Fragments arranged by size:
4169 3259 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7492
XbaI T'CTAG_A
Cuts at: 4828 4828
Size: 7492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4538 4538
Size: 7492
XhoI C'TCGA_G
Cuts at: 1943 2336 1943
Size: 393 7099
XmaI C'CCGG_G
Cuts at: 1870 4817 1870
Size: 2947 4545
XmnI GAAnn'nnTTC
Cuts at: 2109 2517 2676 5621 2109
Size: 408 159 2945 3980
Fragments arranged by size:
3980 2945 408 159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5300 276
Size: 53 83 186 936 3766 2468
Fragments arranged by size:
3766 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PsrI RsrII SacII SexAI SfiI SfoI SrfI
SwaI