Restriction map for
pFN21ASDA0735
(Circular) (Six-base) MAPSORT of: pFN21ASDA0735.seq Check: 658 from: 1 to: 6778
pFN21ASDA0735 6778 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4588 278
Size: 53 83 186 936 3052 2468
Fragments arranged by size:
3052 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2484 4121 1937
Size: 547 1637 4594
Fragments arranged by size:
4594 1637 547
Acc65I G'GTAC_C
Cuts at: 3099 4099 3099
Size: 1000 5778
AclI AA'CG_TT
Cuts at: 1563 4905 5278 1563
Size: 3342 373 3063
Fragments arranged by size:
3342 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2800 2924 4841 6225 6493 1632
Size: 1168 124 1917 1384 268 1917
Fragments arranged by size:
1917 1917 1384 1168 268 124
AfeI AGC'GCT
Cuts at: 3107 3107
Size: 6778
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6581
AflIII A'CryG_T
Cuts at: 1203 2439 3418 5677 6654 1203
Size: 1236 979 2259 977 1327
Fragments arranged by size:
2259 1327 1236 979 977
AgeI A'CCGG_T
Cuts at: 5609 5609
Size: 6778
AhdI GACnn_n'nnGTC
Cuts at: 5507 5507
Size: 6778
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6778
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3775 1184
Size: 2591 4187
AlwNI CAG_nnn'CTG
Cuts at: 3814 4066 4521 6093 3814
Size: 252 455 1572 4499
Fragments arranged by size:
4499 1572 455 252
ApaI G_GGCC'C
Cuts at: 3177 3177
Size: 6778
ApaLI G'TGCA_C
Cuts at: 4835 5991 4835
Size: 1156 5622
ApoI r'AATT_y
Cuts at: 1493 2696 3065 3926 4087 4313 4465 6574 1493
Size: 1203 369 861 161 226 152 2109 1697
Fragments arranged by size:
2109 1697 1203 861 369 226 161 152
AseI AT'TA_AT
Cuts at: 160 1032 5332 160
Size: 872 4300 1606
Fragments arranged by size:
4300 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
AvaI C'yCGr_G
Cuts at: 1870 1943 2706 3674 4053 4103 1870
Size: 73 763 968 379 50 4545
Fragments arranged by size:
4545 968 763 379 73 50
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2161 2194 2161
Size: 33 6745
BamHI G'GATC_C
Cuts at: 2349 4108 4475 2349
Size: 1759 367 4652
Fragments arranged by size:
4652 1759 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2221 2504 3099 3332 3932
Size: 325 217 627 434 283 595 233 600
Cuts at: 3932 4099 5554 618
Size: 167 1455 1842
Fragments arranged by size:
1842 1455 627 600 595 434 325 283
233 217 167
BanII G_rGCy'C
Cuts at: 1391 2924 3177 4097 1391
Size: 1533 253 920 4072
Fragments arranged by size:
4072 1533 920 253
BbeI G_GCGC'C
Cuts at: 2225 2508 3336 3936 2225
Size: 283 828 600 5067
Fragments arranged by size:
5067 828 600 283
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2154 3468 928
Size: 971 255 1314 4238
Fragments arranged by size:
4238 1314 971 255
BbvCI CC'TCA_GC
Cuts at: 2555 2555
Size: 6778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4969 5003 1903
Size: 34 3032 34 3678
Fragments arranged by size:
3678 3032 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2189 3885 4670 5880 2189
Size: 1696 785 1210 3087
Fragments arranged by size:
3087 1696 1210 785
BclI T'GATC_A
Cuts at: 1548 3858 1548
Size: 2310 4468
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2210 2219 2247
Size: 107 122 71 631 605 538 9 28
Cuts at: 2247 2381 5389 136
Size: 134 3008 1525
Fragments arranged by size:
3008 1525 631 605 538 134 122 107
71 28 9
BglII A'GATC_T
Cuts at: 2154 6773 2154
Size: 4619 2159
BlpI GC'TnA_GC
Cuts at: 4195 4195
Size: 6778
Bme1580I G_kGCm'C
Cuts at: 1790 3177 4839 5995 1790
Size: 1387 1662 1156 2573
Fragments arranged by size:
2573 1662 1387 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6778
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2061 2418 2766
Size: 416 53 83 399 24 644 357 348
Cuts at: 2766 4509 5467 442
Size: 1743 958 1753
Fragments arranged by size:
1753 1743 958 644 416 399 357 348
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6778
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2642 2744 2642
Size: 102 6676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2212 2806 2869 3602 5438 1239
Size: 973 594 63 733 1836 2579
Fragments arranged by size:
2579 1836 973 733 594 63
Bpu10I CC'TnA_GC
Cuts at: 2555 3003 2555
Size: 448 6330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2957 3704 4903 5768 6066 2957
Size: 747 1199 865 298 3669
Fragments arranged by size:
3669 1199 865 747 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2303 5441 882
Size: 617 804 3138 2219
Fragments arranged by size:
3138 2219 804 617
BsaAI yAC'GTr
Cuts at: 493 1204 4075 4569 493
Size: 711 2871 494 2702
Fragments arranged by size:
2871 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6772 6772
Size: 6778
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2222 2505 3333 3933
Size: 53 83 186 936 689 283 828 600
Cuts at: 3933 4585 4967 5602 275
Size: 652 382 635 1451
Fragments arranged by size:
1451 936 828 689 652 635 600 382
283 186 83 53
BsaWI w'CCGG_w
Cuts at: 5211 5609 5883 6030 6441 5211
Size: 398 274 147 411 5548
Fragments arranged by size:
5548 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6553
Fragments arranged by size:
6553 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 2733 1186
Size: 1114 433 5231
Fragments arranged by size:
5231 1114 433
BseYI C'CCAG_C
Cuts at: 2536 2737 2808 5981 6477 2536
Size: 201 71 3173 496 2837
Fragments arranged by size:
3173 2837 496 201 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2448 2627 2866 1163
Size: 573 712 179 239 5075
Fragments arranged by size:
5075 712 573 239 179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4228 4989 6017 1540
Size: 275 186 2227 761 1028 2301
Fragments arranged by size:
2301 2227 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2668 2875 3543 4839 4924 5995 2668
Size: 207 668 1296 85 1071 3451
Fragments arranged by size:
3451 1296 1071 668 207 85
BsmI GAATG_Cn'
Cuts at: 2472 3224 3850 3906 4289 4382 2472
Size: 752 626 56 383 93 4868
Fragments arranged by size:
4868 752 626 383 93 56
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2668 2875 2924 3177 3543 4097 4839
Size: 399 878 207 49 253 366 554 742
Cuts at: 4839 4924 5995 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 878 742 554 399 366 253
207 85 49
BspEI T'CCGG_A
Cuts at: 6441 6441
Size: 6778
BspHI T'CATG_A
Cuts at: 4667 6397 4667
Size: 1730 5048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3130 4133 844
Size: 2286 1003 3489
Fragments arranged by size:
3489 2286 1003
BsrBI CCG'CTC
Cuts at: 1394 1823 4665 1394
Size: 429 2842 3507
Fragments arranged by size:
3507 2842 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2095 3801 3831 5273 5447 65
Size: 1183 847 1706 30 1442 174 1396
Fragments arranged by size:
1706 1442 1396 1183 847 174 30
BsrFI r'CCGG_y
Cuts at: 1700 1810 5422 5609 1700
Size: 110 3612 187 2869
Fragments arranged by size:
3612 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6778
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6778
BssSI C'ACGA_G
Cuts at: 4838 5850 4838
Size: 1012 5766
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6778
BstBI TT'CG_AA
Cuts at: 2007 5582 2007
Size: 3575 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6778
BstXI CCAn_nnnn'nTGG
Cuts at: 3058 3374 3580 3997 3058
Size: 316 206 417 5839
Fragments arranged by size:
5839 417 316 206
BstYI r'GATC_y
Cuts at: 1972 2154 2349 3075 3363 4108 4475 4860 4877
Size: 182 195 726 288 745 367 385 17
Cuts at: 4877 6329 6415 6427 6773 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 745 726 385 367 346 288
195 182 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2083 2332 2778 2892 3204 3219
Size: 552 957 61 249 446 114 312 15
Cuts at: 3219 513
Size: 4072
Fragments arranged by size:
4072 957 552 446 312 249 114 61
15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6039
Fragments arranged by size:
6039 683 56
BtsI GCAGTG_nn'
Cuts at: 3815 4294 5088 5108 3815
Size: 479 794 20 5485
Fragments arranged by size:
5485 794 479 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6743
DraI TTT'AAA
Cuts at: 3415 4082 3415
Size: 667 6111
DraIII CAC_nnn'GTG
Cuts at: 2120 2120
Size: 6778
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5785 809
Size: 507 4469 1802
Fragments arranged by size:
4469 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2329 2335 2382
Size: 54 1425 50 275 18 499 6 47
Cuts at: 2382 2568 3201 4225 5114 6743 8
Size: 186 633 1024 889 1629 43
Fragments arranged by size:
1629 1425 1024 889 633 499 275 186
54 50 47 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 4225 1537
Size: 275 2413 4090
Fragments arranged by size:
4090 2413 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2602 2633 4708 1353
Size: 1249 31 2075 3423
Fragments arranged by size:
3423 2075 1249 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5365 5739 5885 387
Size: 1064 138 3776 374 146 1280
Fragments arranged by size:
3776 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2800 2806 2869 2924 3602 4841
Size: 393 580 588 6 63 55 678 1239
Cuts at: 4841 5438 6225 6493 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1239 787 678 597 588 580 393
268 63 55 6
EcoO109I rG'GnC_Cy
Cuts at: 2761 3009 3173 3174 4063 2761
Size: 248 164 1 889 5476
Fragments arranged by size:
5476 889 248 164 1
EcoRI G'AATT_C
Cuts at: 2696 3065 4087 4465 2696
Size: 369 1022 378 5009
Fragments arranged by size:
5009 1022 378 369
FspI TGC'GCA
Cuts at: 5284 5284
Size: 6778
HaeII r_GCGC'y
Cuts at: 1678 2225 2508 3109 3336 3936 5925 1678
Size: 547 283 601 227 600 1989 2531
Fragments arranged by size:
2531 1989 601 600 547 283 227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2844 2876 3343 3375 3455
Size: 32 135 32 1272 32 467 32 80
Cuts at: 3455 3487 4047 4079 5419 5451 5493 5525 1373
Size: 32 560 32 1340 32 42 32 2626
Fragments arranged by size:
2626 1340 1272 560 467 135 80 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4122 4368 669
Size: 988 281 2184 246 3079
Fragments arranged by size:
3079 2184 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6778
HpaI GTT'AAC
Cuts at: 4368 4368
Size: 6778
KasI G'GCGC_C
Cuts at: 2221 2504 3332 3932 2221
Size: 283 828 600 5067
Fragments arranged by size:
5067 828 600 283
KpnI G_GTAC'C
Cuts at: 3103 4103 3103
Size: 1000 5778
MfeI C'AATT_G
Cuts at: 4377 4377
Size: 6778
MluI A'CGCG_T
Cuts at: 6654 6654
Size: 6778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 3060 5892 6076 1305
Size: 816 939 2832 184 2007
Fragments arranged by size:
2832 2007 939 816 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2331 10
Size: 54 1425 343 499 4457
Fragments arranged by size:
4457 1425 499 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2190 2993 3372 3404 4736 5095 5254
Size: 648 1024 803 379 32 1332 359 159
Cuts at: 5254 518
Size: 2042
Fragments arranged by size:
2042 1332 1024 803 648 379 359 159
32
MspA1I CmG'CkG
Cuts at: 1606 2085 4194 4871 6019 6264 1606
Size: 479 2109 677 1148 245 2120
Fragments arranged by size:
2120 2109 1148 677 479 245
NarI GG'CG_CC
Cuts at: 2222 2505 3333 3933 2222
Size: 283 828 600 5067
Fragments arranged by size:
5067 828 600 283
NcoI C'CATG_G
Cuts at: 513 1065 2022 2332 2778 2892 3219 513
Size: 552 957 310 446 114 327 4072
Fragments arranged by size:
4072 957 552 446 327 310 114
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6778
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6778
NotI GC'GGCC_GC
Cuts at: 4225 4225
Size: 6778
NspI r_CATG'y
Cuts at: 1132 1229 2443 2563 3326 3422 4136 5681 1132
Size: 97 1214 120 763 96 714 1545 2229
Fragments arranged by size:
2229 1545 1214 763 714 120 97 96
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6778
PciI A'CATG_T
Cuts at: 2439 3418 5677 2439
Size: 979 2259 3540
Fragments arranged by size:
3540 2259 979
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2784 2804 1108
Size: 1676 20 5082
Fragments arranged by size:
5082 1676 20
PfoI T'CCnGG_A
Cuts at: 3579 3579
Size: 6778
PmeI GTTT'AAAC
Cuts at: 4082 4082
Size: 6778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3040 3530 3562 4847 4879 6360 6392 3008
Size: 32 490 32 1285 32 1481 32 3394
Fragments arranged by size:
3394 1481 1285 490 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3009 4063 3009
Size: 1054 5724
PshAI GACnn'nnGTC
Cuts at: 1613 2442 1613
Size: 829 5949
PsiI TTA'TAA
Cuts at: 4348 4348
Size: 6778
PspOMI G'GGCC_C
Cuts at: 3173 3173
Size: 6778
PstI C_TGCA'G
Cuts at: 830 3754 4130 830
Size: 2924 376 3478
Fragments arranged by size:
3478 2924 376
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6778
SacII CC_GC'GG
Cuts at: 2086 2086
Size: 6778
SalI G'TCGA_C
Cuts at: 1936 4120 1936
Size: 2184 4594
SanDI GG'GwC_CC
Cuts at: 3009 3009
Size: 6778
SbfI CC_TGCA'GG
Cuts at: 4130 4130
Size: 6778
ScaI AGT'ACT
Cuts at: 5026 6738 5026
Size: 1712 5066
SexAI A'CCwGG_T
Cuts at: 3095 3095
Size: 6778
SfcI C'TryA_G
Cuts at: 826 1045 3178 3750 4126 5261 5942 6133 826
Size: 219 2133 572 376 1135 681 191 1471
Fragments arranged by size:
2133 1471 1135 681 572 376 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2210 2381 2210
Size: 171 6607
SfoI GGC'GCC
Cuts at: 2223 2506 3334 3934 2223
Size: 283 828 600 5067
Fragments arranged by size:
5067 828 600 283
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6778
SmaI CCC'GGG
Cuts at: 1872 3676 4105 1872
Size: 1804 429 4545
Fragments arranged by size:
4545 1804 429
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2972 3719 4053 4882 5783 6045
Size: 197 926 1029 747 334 829 901 262
Cuts at: 6045 820
Size: 1553
Fragments arranged by size:
1553 1029 926 901 829 747 334 262
197
SnaBI TAC'GTA
Cuts at: 493 4075 4569 493
Size: 3582 494 2702
Fragments arranged by size:
3582 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6778
SphI G_CATG'C
Cuts at: 1132 4136 1132
Size: 3004 3774
SspI AAT'ATT
Cuts at: 5 52 4702 5
Size: 47 4650 2081
Fragments arranged by size:
4650 2081 47
StuI AGG'CCT
Cuts at: 1517 2523 1517
Size: 1006 5772
StyI C'CwwG_G
Cuts at: 513 1065 2022 2332 2778 2892 2924 3219 4217
Size: 552 957 310 446 114 32 295 998
Cuts at: 4217 513
Size: 3074
Fragments arranged by size:
3074 998 957 552 446 310 295 114
32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2202 4805 4822 4975 5160 5605 325
Size: 1255 622 2603 17 153 185 445 1498
Fragments arranged by size:
2603 1498 1255 622 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3159 3408 3421
Size: 274 80 33 51 1443 1182 249 13
Cuts at: 3421 5024 6736 96
Size: 1603 1712 138
Fragments arranged by size:
1712 1603 1443 1182 274 249 138 80
51 33 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6738 6770 6738
Size: 32 6746
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6778
XbaI T'CTAG_A
Cuts at: 4114 4114
Size: 6778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2805 2805
Size: 6778
XhoI C'TCGA_G
Cuts at: 1943 4053 1943
Size: 2110 4668
XmaI C'CCGG_G
Cuts at: 1870 3674 4103 1870
Size: 1804 429 4545
Fragments arranged by size:
4545 1804 429
XmnI GAAnn'nnTTC
Cuts at: 3023 4907 3023
Size: 1884 4894
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4586 276
Size: 53 83 186 936 3052 2468
Fragments arranged by size:
3052 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacI SapI SrfI SwaI