Restriction map for
pFN21ASDA0733
(Circular) (Six-base) MAPSORT of: pFN21ASDA0733.seq Check: 3115 from: 1 to: 6808
pFN21ASDA0733 6808 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4618 278
Size: 53 83 186 936 3082 2468
Fragments arranged by size:
3082 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2233 2871 4151 1937
Size: 296 638 1280 4594
Fragments arranged by size:
4594 1280 638 296
Acc65I G'GTAC_C
Cuts at: 4129 4129
Size: 6808
AclI AA'CG_TT
Cuts at: 1563 4935 5308 1563
Size: 3372 373 3063
Fragments arranged by size:
3372 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2106 2115 3066 4871 6255 6523 1632
Size: 474 9 951 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1384 951 474 268 9
AfeI AGC'GCT
Cuts at: 3901 3901
Size: 6808
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6611
AflIII A'CryG_T
Cuts at: 1203 3285 3559 5707 6684 1203
Size: 2082 274 2148 977 1327
Fragments arranged by size:
2148 2082 1327 977 274
AgeI A'CCGG_T
Cuts at: 5639 5639
Size: 6808
AhdI GACnn_n'nnGTC
Cuts at: 2796 5537 2796
Size: 2741 4067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6808
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6808
AlwNI CAG_nnn'CTG
Cuts at: 3805 4551 6123 3805
Size: 746 1572 4490
Fragments arranged by size:
4490 1572 746
ApaLI G'TGCA_C
Cuts at: 4865 6021 4865
Size: 1156 5652
ApoI r'AATT_y
Cuts at: 1493 2078 2203 2223 2470 2653 3763 4117 4343
Size: 585 125 20 247 183 1110 354 226
Cuts at: 4343 4495 6604 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1110 585 354 247 226 183
152 125 20
AseI AT'TA_AT
Cuts at: 160 1032 5362 160
Size: 872 4330 1606
Fragments arranged by size:
4330 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
AvaI C'yCGr_G
Cuts at: 1870 1943 3375 3876 4133 1870
Size: 73 1432 501 257 4545
Fragments arranged by size:
4545 1432 501 257 73
BamHI G'GATC_C
Cuts at: 4138 4505 4138
Size: 367 6441
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3747 4129 5584 618
Size: 325 217 627 1960 382 1455 1842
Fragments arranged by size:
1960 1842 1455 627 382 325 217
BanII G_rGCy'C
Cuts at: 1391 4021 4127 1391
Size: 2630 106 4072
Fragments arranged by size:
4072 2630 106
BbeI G_GCGC'C
Cuts at: 3751 3751
Size: 6808
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2931 928
Size: 971 1032 4805
Fragments arranged by size:
4805 1032 971
BbvCI CC'TCA_GC
Cuts at: 2754 2754
Size: 6808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4999 5033 1903
Size: 34 3062 34 3678
Fragments arranged by size:
3678 3062 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2116 4700 5910 2116
Size: 2584 1210 3014
Fragments arranged by size:
3014 2584 1210
BclI T'GATC_A
Cuts at: 1548 3505 1548
Size: 1957 4851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5419 136
Size: 107 122 71 631 605 3747 1525
Fragments arranged by size:
3747 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6803 6803
Size: 6808
BlpI GC'TnA_GC
Cuts at: 4225 4225
Size: 6808
Bme1580I G_kGCm'C
Cuts at: 1790 4869 6025 1790
Size: 3079 1156 2573
Fragments arranged by size:
3079 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6808
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4539 5497 442
Size: 416 53 83 399 24 3122 958 1753
Fragments arranged by size:
3122 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6808
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3052 3262 3282 3812 5468 1239
Size: 1813 210 20 530 1656 2579
Fragments arranged by size:
2579 1813 1656 530 210 20
Bpu10I CC'TnA_GC
Cuts at: 2754 2754
Size: 6808
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2400 2409 3695 3978 4933 5798 6096 2400
Size: 9 1286 283 955 865 298 3112
Fragments arranged by size:
3112 1286 955 865 298 283 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5471 882
Size: 617 3972 2219
Fragments arranged by size:
3972 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4105 4599 493
Size: 711 2901 494 2702
Fragments arranged by size:
2901 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3002 6802 3002
Size: 3800 3008
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3748 4615 4997 5632
Size: 53 83 186 936 2215 867 382 635
Cuts at: 5632 275
Size: 1451
Fragments arranged by size:
2215 1451 936 867 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5241 5639 5913 6060 6471 5241
Size: 398 274 147 411 5578
Fragments arranged by size:
5578 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3412 3442 1178
Size: 30 165 30 2009 30 4544
Fragments arranged by size:
4544 2009 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3363 1186
Size: 2177 4631
BseYI C'CCAG_C
Cuts at: 2724 3896 6011 6507 2724
Size: 1172 2115 496 3025
Fragments arranged by size:
3025 2115 1172 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6235
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4258 5019 6047 1540
Size: 275 186 2257 761 1028 2301
Fragments arranged by size:
2301 2257 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4021 4869 4954 6025 4021
Size: 848 85 1071 4804
Fragments arranged by size:
4804 1071 848 85
BsmI GAATG_Cn'
Cuts at: 2716 3202 4319 4412 2716
Size: 486 1117 93 5112
Fragments arranged by size:
5112 1117 486 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4021 4127 4869 4954 6025 1391
Size: 399 2231 106 742 85 1071 2174
Fragments arranged by size:
2231 2174 1071 742 399 106 85
BspEI T'CCGG_A
Cuts at: 6471 6471
Size: 6808
BspHI T'CATG_A
Cuts at: 4697 6427 4697
Size: 1730 5078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 4163 844
Size: 1232 2087 3489
Fragments arranged by size:
3489 2087 1232
BsrBI CCG'CTC
Cuts at: 1394 1823 4695 1394
Size: 429 2872 3507
Fragments arranged by size:
3507 2872 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5303 5477 65
Size: 1183 4055 174 1396
Fragments arranged by size:
4055 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5452 5639 1700
Size: 110 3642 187 2869
Fragments arranged by size:
3642 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6808
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6808
BssSI C'ACGA_G
Cuts at: 4868 5880 4868
Size: 1012 5796
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3272 794
Size: 2478 4330
BstBI TT'CG_AA
Cuts at: 2007 5612 2007
Size: 3605 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6808
BstYI r'GATC_y
Cuts at: 1972 3599 4138 4505 4890 4907 6359 6445 6457
Size: 1627 539 367 385 17 1452 86 12
Cuts at: 6457 6803 1972
Size: 346 1977
Fragments arranged by size:
1977 1627 1452 539 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3177 3177
Size: 6808
BtgI C'CryG_G
Cuts at: 513 1065 2022 2290 513
Size: 552 957 268 5031
Fragments arranged by size:
5031 957 552 268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6069
Fragments arranged by size:
6069 683 56
BtsI GCAGTG_nn'
Cuts at: 4324 5118 5138 4324
Size: 794 20 5994
Fragments arranged by size:
5994 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6808
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6773
DraI TTT'AAA
Cuts at: 4112 4112
Size: 6808
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5815 809
Size: 507 4499 1802
Fragments arranged by size:
4499 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2365 4255 5144
Size: 54 1425 50 275 18 535 1890 889
Cuts at: 5144 6773 8
Size: 1629 43
Fragments arranged by size:
1890 1629 1425 889 535 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4255 1537
Size: 275 2443 4090
Fragments arranged by size:
4090 2443 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2290 3085 3248 4738 1353
Size: 937 795 163 1490 3423
Fragments arranged by size:
3423 1490 937 795 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5395 5769 5915 387
Size: 1064 138 3806 374 146 1280
Fragments arranged by size:
3806 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4019 4019
Size: 6808
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2115 3052 3066 3262 3282 3812
Size: 393 474 9 937 14 196 20 530
Cuts at: 3812 4871 5468 6255 6523 1239
Size: 1059 597 787 268 1524
Fragments arranged by size:
1524 1059 937 787 597 530 474 393
268 196 20 14 9
EcoO109I rG'GnC_Cy
Cuts at: 2981 3308 3938 2981
Size: 327 630 5851
Fragments arranged by size:
5851 630 327
EcoRI G'AATT_C
Cuts at: 4117 4495 4117
Size: 378 6430
EcoRV GAT'ATC
Cuts at: 2180 2180
Size: 6808
FspI TGC'GCA
Cuts at: 5314 5314
Size: 6808
HaeII r_GCGC'y
Cuts at: 1678 3751 3903 5955 1678
Size: 2073 152 2052 2531
Fragments arranged by size:
2531 2073 2052 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2782 2814 3146
Size: 32 135 32 683 32 495 32 332
Cuts at: 3146 3178 3292 3324 3369 3401 3953 3985 5449
Size: 32 114 32 45 32 552 32 1464
Cuts at: 5449 5481 5523 5555 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1464 683 552 495 332 135 114
45 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3173 4152 4398 669
Size: 988 281 1235 979 246 3079
Fragments arranged by size:
3079 1235 988 979 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6808
HpaI GTT'AAC
Cuts at: 4398 4398
Size: 6808
KasI G'GCGC_C
Cuts at: 3747 3747
Size: 6808
KpnI G_GTAC'C
Cuts at: 4133 4133
Size: 6808
MfeI C'AATT_G
Cuts at: 4407 4407
Size: 6808
MluI A'CGCG_T
Cuts at: 6684 6684
Size: 6808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5922 6106 1305
Size: 4617 184 2007
Fragments arranged by size:
4617 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2367 10
Size: 54 1425 343 535 4451
Fragments arranged by size:
4451 1425 535 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2613 4766 5125 5284 518
Size: 648 1447 2153 359 159 2042
Fragments arranged by size:
2153 2042 1447 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3805 4224 4901 6049 6294 1606
Size: 2199 419 677 1148 245 2120
Fragments arranged by size:
2199 2120 1148 677 419 245
NarI GG'CG_CC
Cuts at: 3748 3748
Size: 6808
NcoI C'CATG_G
Cuts at: 513 1065 2022 2290 513
Size: 552 957 268 5031
Fragments arranged by size:
5031 957 552 268
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6808
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6808
NotI GC'GGCC_GC
Cuts at: 4255 4255
Size: 6808
NspI r_CATG'y
Cuts at: 1132 1229 2119 2878 3289 3563 4166 5711 1132
Size: 97 890 759 411 274 603 1545 2229
Fragments arranged by size:
2229 1545 890 759 603 411 274 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6808
PciI A'CATG_T
Cuts at: 3285 3559 5707 3285
Size: 274 2148 4386
Fragments arranged by size:
4386 2148 274
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2486 1108
Size: 1378 5430
PfoI T'CCnGG_A
Cuts at: 3278 3278
Size: 6808
PmeI GTTT'AAAC
Cuts at: 4112 4112
Size: 6808
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4877 4909 6390 6422 4877
Size: 32 1481 32 5263
Fragments arranged by size:
5263 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2981 3308 3938 2981
Size: 327 630 5851
Fragments arranged by size:
5851 630 327
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6808
PsiI TTA'TAA
Cuts at: 4378 4378
Size: 6808
PspXI vC'TCGA_Gb
Cuts at: 3375 3375
Size: 6808
PstI C_TGCA'G
Cuts at: 830 3448 4160 830
Size: 2618 712 3478
Fragments arranged by size:
3478 2618 712
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6808
PvuII CAG'CTG
Cuts at: 3805 3805
Size: 6808
SacI G_AGCT'C
Cuts at: 4021 4021
Size: 6808
SalI G'TCGA_C
Cuts at: 1936 4150 1936
Size: 2214 4594
SbfI CC_TGCA'GG
Cuts at: 4160 4160
Size: 6808
ScaI AGT'ACT
Cuts at: 5056 6768 5056
Size: 1712 5096
SfcI C'TryA_G
Cuts at: 826 1045 3444 4156 5291 5972 6163 826
Size: 219 2399 712 1135 681 191 1471
Fragments arranged by size:
2399 1471 1135 712 681 219 191
SfoI GGC'GCC
Cuts at: 3749 3749
Size: 6808
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6808
SmaI CCC'GGG
Cuts at: 1872 4135 1872
Size: 2263 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2415 2424 3375 3674 3993 4912
Size: 197 926 472 9 951 299 319 919
Cuts at: 4912 5813 6075 820
Size: 901 262 1553
Fragments arranged by size:
1553 951 926 919 901 472 319 299
262 197 9
SnaBI TAC'GTA
Cuts at: 493 4105 4599 493
Size: 3612 494 2702
Fragments arranged by size:
3612 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6808
SphI G_CATG'C
Cuts at: 1132 4166 1132
Size: 3034 3774
SspI AAT'ATT
Cuts at: 5 52 2971 3529 3925 4732 5
Size: 47 2919 558 396 807 2081
Fragments arranged by size:
2919 2081 807 558 396 47
StuI AGG'CCT
Cuts at: 1517 2671 1517
Size: 1154 5654
StyI C'CwwG_G
Cuts at: 513 1065 2022 2029 2290 4091 4247 513
Size: 552 957 7 261 1801 156 3074
Fragments arranged by size:
3074 1801 957 552 261 156 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4835 4852 5005 5190 5635 325
Size: 1255 3255 17 153 185 445 1498
Fragments arranged by size:
3255 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2171 2632 4040
Size: 274 80 33 51 1443 194 461 1408
Cuts at: 4040 5054 6766 96
Size: 1014 1712 138
Fragments arranged by size:
1712 1443 1408 1014 461 274 194 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6768 6800 6768
Size: 32 6776
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6808
XbaI T'CTAG_A
Cuts at: 4144 4144
Size: 6808
XhoI C'TCGA_G
Cuts at: 1943 3375 1943
Size: 1432 5376
XmaI C'CCGG_G
Cuts at: 1870 4133 1870
Size: 2263 4545
XmnI GAAnn'nnTTC
Cuts at: 2385 3031 3660 4937 2385
Size: 646 629 1277 4256
Fragments arranged by size:
4256 1277 646 629
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4616 276
Size: 53 83 186 936 3082 2468
Fragments arranged by size:
3082 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI