Restriction map for
pFN21ASDA0724
(Circular) (Six-base) MAPSORT of: pFN21ASDA0724.seq Check: 1222 from: 1 to: 7618
pFN21ASDA0724 7618 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3163 3163
Size: 7618
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3468 5428 278
Size: 53 83 186 936 1932 1960 2468
Fragments arranged by size:
2468 1960 1932 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3165 3189 4961 1937
Size: 1228 24 1772 4594
Fragments arranged by size:
4594 1772 1228 24
Acc65I G'GTAC_C
Cuts at: 2233 4939 2233
Size: 2706 4912
AclI AA'CG_TT
Cuts at: 1563 5745 6118 1563
Size: 4182 373 3063
Fragments arranged by size:
4182 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2188 4445 4696 4783 5681 7065 7333 1632
Size: 556 2257 251 87 898 1384 268 1917
Fragments arranged by size:
2257 1917 1384 898 556 268 251 87
AflII C'TTAA_G
Cuts at: 820 1017 3842 820
Size: 197 2825 4596
Fragments arranged by size:
4596 2825 197
AflIII A'CryG_T
Cuts at: 1203 6517 7494 1203
Size: 5314 977 1327
Fragments arranged by size:
5314 1327 977
AgeI A'CCGG_T
Cuts at: 6449 6449
Size: 7618
AhdI GACnn_n'nnGTC
Cuts at: 4271 6347 4271
Size: 2076 5542
AleI CACnn'nnGTG
Cuts at: 1166 4201 4870 1166
Size: 3035 669 3914
Fragments arranged by size:
3914 3035 669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7618
AlwNI CAG_nnn'CTG
Cuts at: 2060 2218 3545 3802 3883 4423 5361 6933 2060
Size: 158 1327 257 81 540 938 1572 2745
Fragments arranged by size:
2745 1572 1327 938 540 257 158 81
ApaI G_GGCC'C
Cuts at: 4371 4371
Size: 7618
ApaLI G'TGCA_C
Cuts at: 5675 6831 5675
Size: 1156 6462
ApoI r'AATT_y
Cuts at: 1493 3285 4927 5153 5305 7414 1493
Size: 1792 1642 226 152 2109 1697
Fragments arranged by size:
2109 1792 1697 1642 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6172 160
Size: 872 5140 1606
Fragments arranged by size:
5140 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7618
AvaI C'yCGr_G
Cuts at: 1870 1943 2982 4943 1870
Size: 73 1039 1961 4545
Fragments arranged by size:
4545 1961 1039 73
AvrII C'CTAG_G
Cuts at: 3634 3634
Size: 7618
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2558 2591 2558
Size: 33 7585
BamHI G'GATC_C
Cuts at: 4948 5315 4948
Size: 367 7251
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2233 2722 3484 4192 4513
Size: 325 217 627 446 489 762 708 321
Cuts at: 4513 4939 6394 618
Size: 426 1455 1842
Fragments arranged by size:
1842 1455 762 708 627 489 446 426
325 321 217
BanII G_rGCy'C
Cuts at: 1391 2787 3402 3495 3568 4371 4937 1391
Size: 1396 615 93 73 803 566 4072
Fragments arranged by size:
4072 1396 803 615 566 93 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2617 4066 4608 928
Size: 971 718 1449 542 3938
Fragments arranged by size:
3938 1449 971 718 542
BbvCI CC'TCA_GC
Cuts at: 2478 4775 2478
Size: 2297 5321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4796 4830 5809 5843 1903
Size: 34 2859 34 979 34 3678
Fragments arranged by size:
3678 2859 979 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4786 5510 6720 4786
Size: 724 1210 5684
Fragments arranged by size:
5684 1210 724
BclI T'GATC_A
Cuts at: 1548 3600 3997 1548
Size: 2052 397 5169
Fragments arranged by size:
5169 2052 397
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2411 6229 136
Size: 107 122 71 631 605 739 3818 1525
Fragments arranged by size:
3818 1525 739 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2277 3681 7613 2277
Size: 1404 3932 2282
Fragments arranged by size:
3932 2282 1404
BlpI GC'TnA_GC
Cuts at: 3350 4097 5035 3350
Size: 747 938 5933
Fragments arranged by size:
5933 938 747
Bme1580I G_kGCm'C
Cuts at: 1790 4371 5679 6835 1790
Size: 2581 1308 1156 2573
Fragments arranged by size:
2581 2573 1308 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7618
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2566 3220 5349
Size: 416 53 83 399 24 1149 654 2129
Cuts at: 5349 6307 442
Size: 958 1753
Fragments arranged by size:
2129 1753 1149 958 654 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7618
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 3421 2995
Size: 426 7192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2082 2311 2657 2686 2738 3905 4652 6278
Size: 843 229 346 29 52 1167 747 1626
Cuts at: 6278 1239
Size: 2579
Fragments arranged by size:
2579 1626 1167 843 747 346 229 52
29
Bpu10I CC'TnA_GC
Cuts at: 2478 3645 4011 4721 4775 2478
Size: 1167 366 710 54 5321
Fragments arranged by size:
5321 1167 710 366 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2675 3659 4484 5743 6608 6906 2675
Size: 984 825 1259 865 298 3387
Fragments arranged by size:
3387 1259 984 865 825 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2188 2924 3077 3449 4886 6281 882
Size: 617 689 736 153 372 1437 1395 2219
Fragments arranged by size:
2219 1437 1395 736 689 617 372 153
BsaAI yAC'GTr
Cuts at: 493 1204 2848 4915 5409 493
Size: 711 1644 2067 494 2702
Fragments arranged by size:
2702 2067 1644 711 494
BsaBI GATnn'nnATC
Cuts at: 3518 7612 3518
Size: 4094 3524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3465 5425 5807 6442
Size: 53 83 186 936 1932 1960 382 635
Cuts at: 6442 275
Size: 1451
Fragments arranged by size:
1960 1932 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6051 6449 6723 6870 7281 6051
Size: 398 274 147 411 6388
Fragments arranged by size:
6388 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4121 4151 1178
Size: 30 165 30 2718 30 4645
Fragments arranged by size:
4645 2718 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2561 3410 3419 3848 3914 1186
Size: 865 510 849 9 429 66 4890
Fragments arranged by size:
4890 865 849 510 429 66 9
BseYI C'CCAG_C
Cuts at: 2657 2666 2706 2881 3370 3802 4213 6821 7317
Size: 9 40 175 489 432 411 2608 496
Cuts at: 7317 2657
Size: 2958
Fragments arranged by size:
2958 2608 496 489 432 411 175 40
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 2732 2933 3034 3200 3551 3836
Size: 573 360 636 201 101 166 351 285
Cuts at: 3836 1163
Size: 4945
Fragments arranged by size:
4945 636 573 360 351 285 201 166
101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4609 5068 5829 6857 1540
Size: 275 186 2608 459 761 1028 2301
Fragments arranged by size:
2608 2301 1028 761 459 275 186
BsiHKAI G_wGCw'C
Cuts at: 2266 2691 2787 3009 3568 5679 5764 6835 2266
Size: 425 96 222 559 2111 85 1071 3049
Fragments arranged by size:
3049 2111 1071 559 425 222 96 85
BsmI GAATG_Cn'
Cuts at: 2821 5129 5222 2821
Size: 2308 93 5217
Fragments arranged by size:
5217 2308 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2266 2691 2787 3009 3402 3495 3568
Size: 399 476 425 96 222 393 93 73
Cuts at: 3568 4371 4937 5679 5764 6835 1391
Size: 803 566 742 85 1071 2174
Fragments arranged by size:
2174 1071 803 742 566 476 425 399
393 222 96 93 85 73
BspEI T'CCGG_A
Cuts at: 7281 7281
Size: 7618
BspHI T'CATG_A
Cuts at: 3597 3988 4000 4288 5507 7237 3597
Size: 391 12 288 1219 1730 3978
Fragments arranged by size:
3978 1730 1219 391 288 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3163 3714 3858 4060 4973 844
Size: 2319 551 144 202 913 3489
Fragments arranged by size:
3489 2319 913 551 202 144
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 5505 1394
Size: 429 206 3476 3507
Fragments arranged by size:
3507 3476 429 206
BsrDI GCAATG_nn'
Cuts at: 65 1248 6113 6287 65
Size: 1183 4865 174 1396
Fragments arranged by size:
4865 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3180 6262 6449 1700
Size: 110 1370 3082 187 2869
Fragments arranged by size:
3082 2869 1370 187 110
BsrGI T'GTAC_A
Cuts at: 96 4224 96
Size: 4128 3490
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7618
BssSI C'ACGA_G
Cuts at: 5678 6690 5678
Size: 1012 6606
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2218 2911 794
Size: 1424 693 5501
Fragments arranged by size:
5501 1424 693
BstBI TT'CG_AA
Cuts at: 2007 6422 2007
Size: 4415 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7618
BstXI CCAn_nnnn'nTGG
Cuts at: 2196 2252 3730 3925 4051 2196
Size: 56 1478 195 126 5763
Fragments arranged by size:
5763 1478 195 126 56
BstYI r'GATC_y
Cuts at: 1972 2241 2277 3681 3762 3837 4809 4948 5315
Size: 269 36 1404 81 75 972 139 367
Cuts at: 5315 5700 5717 7169 7255 7267 7613 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1404 972 385 367 346 269
139 86 81 75 36 17 12
BstZ17I GTA'TAC
Cuts at: 3166 3166
Size: 7618
Bsu36I CC'TnA_GG
Cuts at: 2544 4509 2544
Size: 1965 5653
BtgI C'CryG_G
Cuts at: 513 1065 2022 2938 3509 4050 4188 4885 513
Size: 552 957 916 571 541 138 697 3246
Fragments arranged by size:
3246 957 916 697 571 552 541 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2938 3433 487
Size: 683 56 1712 495 4672
Fragments arranged by size:
4672 1712 683 495 56
BtsI GCAGTG_nn'
Cuts at: 2803 2916 3119 4632 4705 4865 5134 5928 5948
Size: 113 203 1513 73 160 269 794 20
Cuts at: 5948 2803
Size: 4473
Fragments arranged by size:
4473 1513 794 269 203 160 113 73
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7618
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7583
DraI TTT'AAA
Cuts at: 4922 4922
Size: 7618
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3593 6625 809
Size: 507 2277 3032 1802
Fragments arranged by size:
3032 2277 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2438 3064 3182
Size: 54 1425 50 275 18 608 626 118
Cuts at: 3182 3927 5065 5954 7583 8
Size: 745 1138 889 1629 43
Fragments arranged by size:
1629 1425 1138 889 745 626 608 275
118 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5065 1537
Size: 275 3253 4090
Fragments arranged by size:
4090 3253 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 2342 2474 2792 3664 3828 3968 4328
Size: 918 71 132 318 872 164 140 360
Cuts at: 4328 4361 5548 1353
Size: 33 1187 3423
Fragments arranged by size:
3423 1187 918 872 360 318 164 140
132 71 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6205 6579 6725 387
Size: 1064 138 4616 374 146 1280
Fragments arranged by size:
4616 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2785 3566 2785
Size: 781 6837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2188 2311 2657 2686 2738 3905
Size: 393 450 106 123 346 29 52 1167
Cuts at: 3905 4445 4652 4696 4783 5681 6278 7065 7333
Size: 540 207 44 87 898 597 787 268
Cuts at: 7333 1239
Size: 1524
Fragments arranged by size:
1524 1167 898 787 597 540 450 393
346 268 207 123 106 87 52 44
29
EcoNI CCTnn'n_nnAGG
Cuts at: 4415 4415
Size: 7618
EcoO109I rG'GnC_Cy
Cuts at: 3424 4007 4040 4318 4367 4741 3424
Size: 583 33 278 49 374 6301
Fragments arranged by size:
6301 583 374 278 49 33
EcoRI G'AATT_C
Cuts at: 4927 5305 4927
Size: 378 7240
EcoRV GAT'ATC
Cuts at: 4684 4684
Size: 7618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4726 4813 4726
Size: 87 7531
FspI TGC'GCA
Cuts at: 6124 6124
Size: 7618
HaeII r_GCGC'y
Cuts at: 1678 2111 2747 3803 6765 1678
Size: 433 636 1056 2962 2531
Fragments arranged by size:
2962 2531 1056 636 433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3122 3154 3895 3927 4201
Size: 32 135 32 1550 32 741 32 274
Cuts at: 4201 4233 4257 4289 4449 4481 6259 6291 6333
Size: 32 24 32 160 32 1778 32 42
Cuts at: 6333 6365 1373
Size: 32 2626
Fragments arranged by size:
2626 1778 1550 741 274 160 135 42
32 32 32 32 32 32 32 32
32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 3034 3469 4962 5208 669
Size: 988 281 1096 435 1493 246 3079
Fragments arranged by size:
3079 1493 1096 988 435 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7618
HpaI GTT'AAC
Cuts at: 5208 5208
Size: 7618
KpnI G_GTAC'C
Cuts at: 2237 4943 2237
Size: 2706 4912
MfeI C'AATT_G
Cuts at: 4311 5217 4311
Size: 906 6712
MluI A'CGCG_T
Cuts at: 7494 7494
Size: 7618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3942 3981 4199 6732 6916 1305
Size: 2637 39 218 2533 184 2007
Fragments arranged by size:
2637 2533 2007 218 184 39
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2440 3066 3929 10
Size: 54 1425 343 608 626 863 3699
Fragments arranged by size:
3699 1425 863 626 608 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2957 3041 3314 3548 3993 4201 4625
Size: 648 1791 84 273 234 445 208 424
Cuts at: 4625 4870 5576 5935 6094 518
Size: 245 706 359 159 2042
Fragments arranged by size:
2042 1791 706 648 445 424 359 273
245 234 208 159 84
MspA1I CmG'CkG
Cuts at: 1606 2208 2508 2632 2644 2670 2706 2716 2728
Size: 602 300 124 12 26 36 10 12
Cuts at: 2728 2875 3199 3535 4201 4270 4904 5034 5711
Size: 147 324 336 666 69 634 130 677
Cuts at: 5711 6859 7104 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 666 634 602 336 324
300 245 147 130 124 69 36 26
12 12 10
NcoI C'CATG_G
Cuts at: 513 1065 2022 2938 4188 4885 513
Size: 552 957 916 1250 697 3246
Fragments arranged by size:
3246 1250 957 916 697 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7618
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7618
NotI GC'GGCC_GC
Cuts at: 5065 5065
Size: 7618
NruI TCG'CGA
Cuts at: 4818 4818
Size: 7618
NspI r_CATG'y
Cuts at: 1132 1229 3632 3787 4729 4976 6521 1132
Size: 97 2403 155 942 247 1545 2229
Fragments arranged by size:
2403 2229 1545 942 247 155 97
PasI CC'CwG_GG
Cuts at: 1348 2965 4371 1348
Size: 1617 1406 4595
Fragments arranged by size:
4595 1617 1406
PciI A'CATG_T
Cuts at: 6517 6517
Size: 7618
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2495 3202 3883 4126 1108
Size: 1387 707 681 243 4600
Fragments arranged by size:
4600 1387 707 681 243
PmeI GTTT'AAAC
Cuts at: 4922 4922
Size: 7618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5687 5719 7200 7232 3839
Size: 32 1816 32 1481 32 4225
Fragments arranged by size:
4225 1816 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4040 4040
Size: 7618
PshAI GACnn'nnGTC
Cuts at: 1613 3981 1613
Size: 2368 5250
PsiI TTA'TAA
Cuts at: 5188 5188
Size: 7618
PspOMI G'GGCC_C
Cuts at: 4367 4367
Size: 7618
PstI C_TGCA'G
Cuts at: 830 2056 2223 2733 2775 2892 3126 4068 4749
Size: 1226 167 510 42 117 234 942 681
Cuts at: 4749 4970 830
Size: 221 3478
Fragments arranged by size:
3478 1226 942 681 510 234 221 167
117 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7618
PvuII CAG'CTG
Cuts at: 2208 2644 2670 2706 2716 3199 3535 4201 4270
Size: 436 26 36 10 483 336 666 69
Cuts at: 4270 4904 2208
Size: 634 4922
Fragments arranged by size:
4922 666 634 483 436 336 69 36
26 10
SacI G_AGCT'C
Cuts at: 2787 3568 2787
Size: 781 6837
SalI G'TCGA_C
Cuts at: 1936 4960 1936
Size: 3024 4594
SanDI GG'GwC_CC
Cuts at: 4040 4040
Size: 7618
SapI GCTCTTCn'nnn_
Cuts at: 2271 2342 2792 3828 2271
Size: 71 450 1036 6061
Fragments arranged by size:
6061 1036 450 71
SbfI CC_TGCA'GG
Cuts at: 4970 4970
Size: 7618
ScaI AGT'ACT
Cuts at: 2249 5866 7578 2249
Size: 3617 1712 2289
Fragments arranged by size:
3617 2289 1712
SexAI A'CCwGG_T
Cuts at: 4753 4753
Size: 7618
SfcI C'TryA_G
Cuts at: 826 1045 2052 2219 2648 2729 2771 2888 3122
Size: 219 1007 167 429 81 42 117 234
Cuts at: 3122 4064 4745 4966 6101 6782 6973 826
Size: 942 681 221 1135 681 191 1471
Fragments arranged by size:
1471 1135 1007 942 681 681 429 234
221 219 191 167 117 81 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7618
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7618
SmaI CCC'GGG
Cuts at: 1872 2984 4945 1872
Size: 1112 1961 4545
Fragments arranged by size:
4545 1961 1112
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2690 3674 3842 4499 5722 6623
Size: 197 926 747 984 168 657 1223 901
Cuts at: 6623 6885 820
Size: 262 1553
Fragments arranged by size:
1553 1223 984 926 901 747 657 262
197 168
SnaBI TAC'GTA
Cuts at: 493 4915 5409 493
Size: 4422 494 2702
Fragments arranged by size:
4422 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7618
SphI G_CATG'C
Cuts at: 1132 3632 4976 1132
Size: 2500 1344 3774
Fragments arranged by size:
3774 2500 1344
SspI AAT'ATT
Cuts at: 5 52 5542 5
Size: 47 5490 2081
Fragments arranged by size:
5490 2081 47
StuI AGG'CCT
Cuts at: 1517 2195 2586 4244 4529 4652 1517
Size: 678 391 1658 285 123 4483
Fragments arranged by size:
4483 1658 678 391 285 123
StyI C'CwwG_G
Cuts at: 513 1065 2022 2076 2938 3618 3634 4188 4885
Size: 552 957 54 862 680 16 554 697
Cuts at: 4885 5057 513
Size: 172 3074
Fragments arranged by size:
3074 957 862 697 680 554 552 172
54 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 2680 2698 5645 5662 5815 6000
Size: 1255 777 323 18 2947 17 153 185
Cuts at: 6000 6445 325
Size: 445 1498
Fragments arranged by size:
2947 1498 1255 777 445 323 185 153
18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 2377 4224
Size: 274 80 33 51 1443 270 130 1847
Cuts at: 4224 5864 7576 96
Size: 1640 1712 138
Fragments arranged by size:
1847 1712 1640 1443 274 270 138 130
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3858 3890 4121 4153 4743 4775 7578 7610 3858
Size: 32 231 32 590 32 2803 32 3866
Fragments arranged by size:
3866 2803 590 231 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3545 1316
Size: 2229 5389
XbaI T'CTAG_A
Cuts at: 4954 4954
Size: 7618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 4051 2605
Size: 1446 6172
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7618
XmaI C'CCGG_G
Cuts at: 1870 2982 4943 1870
Size: 1112 1961 4545
Fragments arranged by size:
4545 1961 1112
XmnI GAAnn'nnTTC
Cuts at: 4859 5747 4859
Size: 888 6730
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3466 5426 276
Size: 53 83 186 936 1932 1960 2468
Fragments arranged by size:
2468 1960 1932 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BfrBI BsiWI BsmBI DraIII
FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacII SfiI SfoI
SrfI SwaI