Restriction map for
pFN21ASDA0723
(Circular) (Six-base) MAPSORT of: pFN21ASDA0723.seq Check: 4418 from: 1 to: 7270
pFN21ASDA0723 7270 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5080 278
Size: 53 83 186 936 3544 2468
Fragments arranged by size:
3544 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3065 4613 1937
Size: 1128 1548 4594
Fragments arranged by size:
4594 1548 1128
Acc65I G'GTAC_C
Cuts at: 4591 4591
Size: 7270
AclI AA'CG_TT
Cuts at: 1563 5397 5770 1563
Size: 3834 373 3063
Fragments arranged by size:
3834 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2493 3483 4005 4125 5333 6717 6985 1632
Size: 861 990 522 120 1208 1384 268 1917
Fragments arranged by size:
1917 1384 1208 990 861 522 268 120
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7073
AflIII A'CryG_T
Cuts at: 1203 3508 6169 7146 1203
Size: 2305 2661 977 1327
Fragments arranged by size:
2661 2305 1327 977
AgeI A'CCGG_T
Cuts at: 6101 6101
Size: 7270
AhdI GACnn_n'nnGTC
Cuts at: 5999 5999
Size: 7270
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7270
AlwNI CAG_nnn'CTG
Cuts at: 4256 5013 6585 4256
Size: 757 1572 4941
Fragments arranged by size:
4941 1572 757
ApaLI G'TGCA_C
Cuts at: 2235 5327 6483 2235
Size: 3092 1156 3022
Fragments arranged by size:
3092 3022 1156
ApoI r'AATT_y
Cuts at: 1493 2431 2756 3084 4517 4579 4805 4957 7066
Size: 938 325 328 1433 62 226 152 2109
Cuts at: 7066 1493
Size: 1697
Fragments arranged by size:
2109 1697 1433 938 328 325 226 152
62
AseI AT'TA_AT
Cuts at: 160 1032 3324 5824 160
Size: 872 2292 2500 1606
Fragments arranged by size:
2500 2292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7270
AvaI C'yCGr_G
Cuts at: 1870 1943 4595 1870
Size: 73 2652 4545
Fragments arranged by size:
4545 2652 73
AvrII C'CTAG_G
Cuts at: 2543 2543
Size: 7270
BamHI G'GATC_C
Cuts at: 4600 4967 4600
Size: 367 6903
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4591 6046 618
Size: 325 217 627 2804 1455 1842
Fragments arranged by size:
2804 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2230 4589 1391
Size: 839 2359 4072
Fragments arranged by size:
4072 2359 839
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2235 2865 3878 928
Size: 971 336 630 1013 4320
Fragments arranged by size:
4320 1013 971 630 336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5461 5495 1903
Size: 34 3524 34 3678
Fragments arranged by size:
3678 3524 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5162 6372 5162
Size: 1210 6060
BclI T'GATC_A
Cuts at: 1548 3487 4192 1548
Size: 1939 705 4626
Fragments arranged by size:
4626 1939 705
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5881 136
Size: 107 122 71 631 605 4209 1525
Fragments arranged by size:
4209 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2510 2810 3806 7265 2510
Size: 300 996 3459 2515
Fragments arranged by size:
3459 2515 996 300
BlpI GC'TnA_GC
Cuts at: 3573 4687 3573
Size: 1114 6156
Bme1580I G_kGCm'C
Cuts at: 1790 2239 5331 6487 1790
Size: 449 3092 1156 2573
Fragments arranged by size:
3092 2573 1156 449
BmgBI CAC'GTC
Cuts at: 1318 2786 3509 1318
Size: 1468 723 5079
Fragments arranged by size:
5079 1468 723
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2116 2609 5001
Size: 416 53 83 399 24 699 493 2392
Cuts at: 5001 5959 442
Size: 958 1753
Fragments arranged by size:
2392 1753 958 699 493 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7270
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 2853 2321
Size: 532 6738
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3801 5930 1239
Size: 2562 2129 2579
Fragments arranged by size:
2579 2562 2129
Bpu10I CC'TnA_GC
Cuts at: 3363 3363
Size: 7270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2509 2631 3274 3548 5395 6260 6558 2509
Size: 122 643 274 1847 865 298 3221
Fragments arranged by size:
3221 1847 865 643 298 274 122
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2729 4049 5933 882
Size: 617 1230 1320 1884 2219
Fragments arranged by size:
2219 1884 1320 1230 617
BsaAI yAC'GTr
Cuts at: 493 1204 4567 5061 493
Size: 711 3363 494 2702
Fragments arranged by size:
3363 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7264 7264
Size: 7270
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5077 5459 6094 275
Size: 53 83 186 936 3544 382 635 1451
Fragments arranged by size:
3544 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3914 5703 6101 6375 6522 6933 3914
Size: 1789 398 274 147 411 4251
Fragments arranged by size:
4251 1789 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7045
Fragments arranged by size:
7045 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2479 2829 1186
Size: 1293 350 5627
Fragments arranged by size:
5627 1293 350
BseYI C'CCAG_C
Cuts at: 6473 6969 6473
Size: 496 6774
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2154 1163
Size: 573 418 6279
Fragments arranged by size:
6279 573 418
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4720 5481 6509 1540
Size: 275 186 2719 761 1028 2301
Fragments arranged by size:
2719 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2230 2239 2903 5331 5416 6487 2230
Size: 9 664 2428 85 1071 3013
Fragments arranged by size:
3013 2428 1071 664 85 9
BsmI GAATG_Cn'
Cuts at: 3535 4781 4874 3535
Size: 1246 93 5931
Fragments arranged by size:
5931 1246 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4044 4044
Size: 7270
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2230 2239 2903 4589 5331 5416 6487
Size: 399 440 9 664 1686 742 85 1071
Cuts at: 6487 1391
Size: 2174
Fragments arranged by size:
2174 1686 1071 742 664 440 399 85
9
BspEI T'CCGG_A
Cuts at: 6933 6933
Size: 7270
BspHI T'CATG_A
Cuts at: 5159 6889 5159
Size: 1730 5540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3177 4625 844
Size: 2333 1448 3489
Fragments arranged by size:
3489 2333 1448
BsrBI CCG'CTC
Cuts at: 1394 1823 5157 1394
Size: 429 3334 3507
Fragments arranged by size:
3507 3334 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3663 3669 4480 5765 5939 65
Size: 1183 2415 6 811 1285 174 1396
Fragments arranged by size:
2415 1396 1285 1183 811 174 6
BsrFI r'CCGG_y
Cuts at: 1700 1810 5914 6101 1700
Size: 110 4104 187 2869
Fragments arranged by size:
4104 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7270
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7270
BssSI C'ACGA_G
Cuts at: 5330 6342 5330
Size: 1012 6258
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2177 794
Size: 1383 5887
BstBI TT'CG_AA
Cuts at: 2007 6074 2007
Size: 4067 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7270
BstYI r'GATC_y
Cuts at: 1972 2510 2810 3806 4600 4967 5352 5369 6821
Size: 538 300 996 794 367 385 17 1452
Cuts at: 6821 6907 6919 7265 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 996 794 538 385 367 346
300 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2326 2632 2866 513
Size: 552 1261 306 234 4917
Fragments arranged by size:
4917 1261 552 306 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6531
Fragments arranged by size:
6531 683 56
BtsI GCAGTG_nn'
Cuts at: 3378 4022 4786 5580 5600 3378
Size: 644 764 794 20 5048
Fragments arranged by size:
5048 794 764 644 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7270
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7235
DraI TTT'AAA
Cuts at: 4574 4574
Size: 7270
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6277 809
Size: 507 4961 1802
Fragments arranged by size:
4961 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 4717 5606
Size: 54 1425 50 275 18 528 2359 889
Cuts at: 5606 7235 8
Size: 1629 43
Fragments arranged by size:
2359 1629 1425 889 528 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4717 1537
Size: 275 2905 4090
Fragments arranged by size:
4090 2905 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2572 3900 5200 1353
Size: 1219 1328 1300 3423
Fragments arranged by size:
3423 1328 1300 1219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5857 6231 6377 387
Size: 1064 138 4268 374 146 1280
Fragments arranged by size:
4268 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2228 2228
Size: 7270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2493 3483 3801 4005 4125 5333 5930
Size: 393 861 990 318 204 120 1208 597
Cuts at: 5930 6717 6985 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1208 990 861 787 597 393 318
268 204 120
EcoNI CCTnn'n_nnAGG
Cuts at: 4416 4416
Size: 7270
EcoO109I rG'GnC_Cy
Cuts at: 2480 3092 3176 2480
Size: 612 84 6574
Fragments arranged by size:
6574 612 84
EcoRI G'AATT_C
Cuts at: 3084 4579 4957 3084
Size: 1495 378 5397
Fragments arranged by size:
5397 1495 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3642 3642
Size: 7270
FspI TGC'GCA
Cuts at: 5776 5776
Size: 7270
HaeII r_GCGC'y
Cuts at: 1678 2838 6417 1678
Size: 1160 3579 2531
Fragments arranged by size:
3579 2531 1160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2389 2421 2495 2527 3604
Size: 32 135 32 817 32 74 32 1077
Cuts at: 3604 3636 3833 3865 5911 5943 5985 6017 1373
Size: 32 197 32 2046 32 42 32 2626
Fragments arranged by size:
2626 2046 1077 817 197 135 74 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2945 3670 3721 4614 4860 669
Size: 988 281 1007 725 51 893 246 3079
Fragments arranged by size:
3079 1007 988 893 725 281 246 51
HindIII A'AGCT_T
Cuts at: 748 3566 4160 748
Size: 2818 594 3858
Fragments arranged by size:
3858 2818 594
HpaI GTT'AAC
Cuts at: 4860 4860
Size: 7270
KpnI G_GTAC'C
Cuts at: 4595 4595
Size: 7270
MfeI C'AATT_G
Cuts at: 4869 4869
Size: 7270
MluI A'CGCG_T
Cuts at: 7146 7146
Size: 7270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3112 4194 6384 6568 1305
Size: 1807 1082 2190 184 2007
Fragments arranged by size:
2190 2007 1807 1082 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 10
Size: 54 1425 343 528 4920
Fragments arranged by size:
4920 1425 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2076 2952 3605 5228 5587 5746 518
Size: 648 910 876 653 1623 359 159 2042
Fragments arranged by size:
2042 1623 910 876 653 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3274 4686 5363 6511 6756 1606
Size: 1668 1412 677 1148 245 2120
Fragments arranged by size:
2120 1668 1412 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2632 2866 513
Size: 552 1567 234 4917
Fragments arranged by size:
4917 1567 552 234
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7270
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7270
NotI GC'GGCC_GC
Cuts at: 4717 4717
Size: 7270
NspI r_CATG'y
Cuts at: 1132 1229 3190 4628 6173 1132
Size: 97 1961 1438 1545 2229
Fragments arranged by size:
2229 1961 1545 1438 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7270
PciI A'CATG_T
Cuts at: 6169 6169
Size: 7270
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3131 1108
Size: 2023 5247
PmeI GTTT'AAAC
Cuts at: 4574 4574
Size: 7270
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5339 5371 6852 6884 5339
Size: 32 1481 32 5725
Fragments arranged by size:
5725 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3092 3176 3092
Size: 84 7186
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7270
PsiI TTA'TAA
Cuts at: 4840 4840
Size: 7270
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4423 4455 4423
Size: 32 7238
PstI C_TGCA'G
Cuts at: 830 4622 830
Size: 3792 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7270
PvuII CAG'CTG
Cuts at: 3274 3274
Size: 7270
SacI G_AGCT'C
Cuts at: 2230 2230
Size: 7270
SalI G'TCGA_C
Cuts at: 1936 4612 1936
Size: 2676 4594
SbfI CC_TGCA'GG
Cuts at: 4622 4622
Size: 7270
ScaI AGT'ACT
Cuts at: 2254 5518 7230 2254
Size: 3264 1712 2294
Fragments arranged by size:
3264 2294 1712
SexAI A'CCwGG_T
Cuts at: 4249 4249
Size: 7270
SfcI C'TryA_G
Cuts at: 826 1045 2450 4618 5753 6434 6625 826
Size: 219 1405 2168 1135 681 191 1471
Fragments arranged by size:
2168 1471 1405 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7270
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7270
SmaI CCC'GGG
Cuts at: 1872 4597 1872
Size: 2725 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2488 2646 3253 3563 5374 6275
Size: 197 926 545 158 607 310 1811 901
Cuts at: 6275 6537 820
Size: 262 1553
Fragments arranged by size:
1811 1553 926 901 607 545 310 262
197 158
SnaBI TAC'GTA
Cuts at: 493 4567 5061 493
Size: 4074 494 2702
Fragments arranged by size:
4074 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7270
SphI G_CATG'C
Cuts at: 1132 4628 1132
Size: 3496 3774
SspI AAT'ATT
Cuts at: 5 52 2854 5194 5
Size: 47 2802 2340 2081
Fragments arranged by size:
2802 2340 2081 47
StuI AGG'CCT
Cuts at: 1517 2103 1517
Size: 586 6684
StyI C'CwwG_G
Cuts at: 513 1065 2543 2632 2866 4709 513
Size: 552 1478 89 234 1843 3074
Fragments arranged by size:
3074 1843 1478 552 234 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3029 3140 4073 5297 5314 5467 5652
Size: 1255 1449 111 933 1224 17 153 185
Cuts at: 5652 6097 325
Size: 445 1498
Fragments arranged by size:
1498 1449 1255 1224 933 445 185 153
111 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2252 5516 7228
Size: 274 80 33 51 1443 275 3264 1712
Cuts at: 7228 96
Size: 138
Fragments arranged by size:
3264 1712 1443 275 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7230 7262 7230
Size: 32 7238
Tth111I GACn'n_nGTC
Cuts at: 1316 2787 1316
Size: 1471 5799
XbaI T'CTAG_A
Cuts at: 4606 4606
Size: 7270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2353 2353
Size: 7270
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7270
XmaI C'CCGG_G
Cuts at: 1870 4595 1870
Size: 2725 4545
XmnI GAAnn'nnTTC
Cuts at: 2203 2641 4138 5399 2203
Size: 438 1497 1261 4074
Fragments arranged by size:
4074 1497 1261 438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5078 276
Size: 53 83 186 936 3544 2468
Fragments arranged by size:
3544 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI