Restriction map for
pFN21ASDA0722
(Circular) (Six-base) MAPSORT of: pFN21ASDA0722.seq Check: 5362 from: 1 to: 7879
pFN21ASDA0722 7879 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3285 3322 4813 3285
Size: 37 1491 6351
Fragments arranged by size:
6351 1491 37
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5689 278
Size: 53 83 186 936 4153 2468
Fragments arranged by size:
4153 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2286 2655 5222 1937
Size: 349 369 2567 4594
Fragments arranged by size:
4594 2567 369 349
Acc65I G'GTAC_C
Cuts at: 2532 5200 2532
Size: 2668 5211
AclI AA'CG_TT
Cuts at: 1563 6006 6379 1563
Size: 4443 373 3063
Fragments arranged by size:
4443 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2243 4631 4754 4948 5942 7326 7594 1632
Size: 611 2388 123 194 994 1384 268 1917
Fragments arranged by size:
2388 1917 1384 994 611 268 194 123
AfeI AGC'GCT
Cuts at: 4898 4898
Size: 7879
AflII C'TTAA_G
Cuts at: 820 1017 4130 820
Size: 197 3113 4569
Fragments arranged by size:
4569 3113 197
AflIII A'CryG_T
Cuts at: 1203 6778 7755 1203
Size: 5575 977 1327
Fragments arranged by size:
5575 1327 977
AgeI A'CCGG_T
Cuts at: 6710 6710
Size: 7879
AhdI GACnn_n'nnGTC
Cuts at: 6608 6608
Size: 7879
AleI CACnn'nnGTG
Cuts at: 1166 3332 1166
Size: 2166 5713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5010 1184
Size: 3826 4053
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4994 5026 4994
Size: 32 7847
AlwNI CAG_nnn'CTG
Cuts at: 2197 2665 2707 2992 3946 4421 4879 5622 7194
Size: 468 42 285 954 475 458 743 1572
Cuts at: 7194 2197
Size: 2882
Fragments arranged by size:
2882 1572 954 743 475 468 458 285
42
ApaI G_GGCC'C
Cuts at: 4052 4085 4328 4355 4401 4643 4052
Size: 33 243 27 46 242 7288
Fragments arranged by size:
7288 243 242 46 33 27
ApaLI G'TGCA_C
Cuts at: 5936 7092 5936
Size: 1156 6723
ApoI r'AATT_y
Cuts at: 1493 5188 5414 5566 7675 1493
Size: 3695 226 152 2109 1697
Fragments arranged by size:
3695 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6433 160
Size: 872 5401 1606
Fragments arranged by size:
5401 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
AvaI C'yCGr_G
Cuts at: 1870 1943 2591 2754 2914 3045 3644 4162 4300
Size: 73 648 163 160 131 599 518 138
Cuts at: 4300 5204 1870
Size: 904 4545
Fragments arranged by size:
4545 904 648 599 518 163 160 138
131 73
BamHI G'GATC_C
Cuts at: 5209 5576 5209
Size: 367 7512
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2393 2532 2645 2677
Size: 325 217 627 309 297 139 113 32
Cuts at: 2677 3136 3302 4256 4390 5200 6655 618
Size: 459 166 954 134 810 1455 1842
Fragments arranged by size:
1842 1455 954 810 627 459 325 309
297 217 166 139 134 113 32
BanII G_rGCy'C
Cuts at: 1391 2031 3185 3836 3890 4052 4085 4246 4295
Size: 640 1154 651 54 162 33 161 49
Cuts at: 4295 4328 4355 4401 4428 4643 5198 1391
Size: 33 27 46 27 215 555 4072
Fragments arranged by size:
4072 1154 651 640 555 215 162 161
54 49 46 33 33 27 27
BbeI G_GCGC'C
Cuts at: 2100 2397 2681 3140 3306 2100
Size: 297 284 459 166 6673
Fragments arranged by size:
6673 459 297 284 166
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2104 3005 3932 4273 928
Size: 971 205 901 927 341 4534
Fragments arranged by size:
4534 971 927 901 341 205
BbvCI CC'TCA_GC
Cuts at: 3132 4210 3132
Size: 1078 6801
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2809 2843 4448 4482 6070 6104 1903
Size: 34 872 34 1605 34 1588 34 3678
Fragments arranged by size:
3678 1605 1588 872 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5771 6981 5771
Size: 1210 6669
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7879
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 3014 3276
Size: 107 122 71 631 605 368 974 262
Cuts at: 3276 3435 4089 4359 4628 6490 136
Size: 159 654 270 269 1862 1525
Fragments arranged by size:
1862 1525 974 654 631 605 368 270
269 262 159 122 107 71
BglII A'GATC_T
Cuts at: 7874 7874
Size: 7879
BlpI GC'TnA_GC
Cuts at: 2351 3474 4691 5296 2351
Size: 1123 1217 605 4934
Fragments arranged by size:
4934 1217 1123 605
Bme1580I G_kGCm'C
Cuts at: 1790 2901 3909 4052 4085 4259 4328 4355 4401
Size: 1111 1008 143 33 174 69 27 46
Cuts at: 4401 4643 5940 7096 1790
Size: 242 1297 1156 2573
Fragments arranged by size:
2573 1297 1156 1111 1008 242 174 143
69 46 33 27
BmgBI CAC'GTC
Cuts at: 1318 3715 4591 1318
Size: 2397 876 4606
Fragments arranged by size:
4606 2397 876
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3135 3252 3314
Size: 416 53 83 399 24 1718 117 62
Cuts at: 3314 3373 5610 6568 442
Size: 59 2237 958 1753
Fragments arranged by size:
2237 1753 1718 958 416 399 117 83
62 59 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 2917 3271 3592 4419 4499 4616 5084
Size: 1283 395 354 321 827 80 117 468
Cuts at: 5084 6539 1239
Size: 1455 2579
Fragments arranged by size:
2579 1455 1283 827 468 395 354 321
117 80
Bpu10I CC'TnA_GC
Cuts at: 3132 3558 4210 4422 4880 3132
Size: 426 652 212 458 6131
Fragments arranged by size:
6131 652 458 426 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3525 6004 6869 7167 3525
Size: 2479 865 298 4237
Fragments arranged by size:
4237 2479 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2042 2753 4514 6542 882
Size: 617 543 711 1761 2028 2219
Fragments arranged by size:
2219 2028 1761 711 617 543
BsaAI yAC'GTr
Cuts at: 493 1204 5176 5670 493
Size: 711 3972 494 2702
Fragments arranged by size:
3972 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7873 7873
Size: 7879
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2394 2678 3137
Size: 53 83 186 936 564 297 284 459
Cuts at: 3137 3303 3486 3581 3983 5686 6068 6703 275
Size: 166 183 95 402 1703 382 635 1451
Fragments arranged by size:
1703 1451 936 635 564 459 402 382
297 284 186 183 166 95 83 53
BsaWI w'CCGG_w
Cuts at: 3996 6312 6710 6984 7131 7542 3996
Size: 2316 398 274 147 411 4333
Fragments arranged by size:
4333 2316 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2945 2975 3214 3244 3520
Size: 30 165 30 1542 30 239 30 276
Cuts at: 3520 3550 1178
Size: 30 5507
Fragments arranged by size:
5507 1542 276 239 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3528 3625 4532 1186
Size: 2342 97 907 4533
Fragments arranged by size:
4533 2342 907 97
BseYI C'CCAG_C
Cuts at: 2200 2607 3670 3792 3932 4250 4414 7082 7578
Size: 407 1063 122 140 318 164 2668 496
Cuts at: 7578 2200
Size: 2501
Fragments arranged by size:
2668 2501 1063 496 407 318 164 140
122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2393 3020 3193 3356 3624 3668
Size: 573 582 75 627 173 163 268 44
Cuts at: 3668 4217 4604 4997 5006 1163
Size: 549 387 393 9 4036
Fragments arranged by size:
4036 627 582 573 549 393 387 268
173 163 75 44 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2035 2476 3220 5329 6090 7118
Size: 275 186 34 441 744 2109 761 1028
Cuts at: 7118 1540
Size: 2301
Fragments arranged by size:
2301 2109 1028 761 744 441 275 186
34
BsiHKAI G_wGCw'C
Cuts at: 3185 3651 4313 4428 4542 5940 6025 7096 3185
Size: 466 662 115 114 1398 85 1071 3968
Fragments arranged by size:
3968 1398 1071 662 466 115 114 85
BsmI GAATG_Cn'
Cuts at: 4932 5390 5483 4932
Size: 458 93 7328
Fragments arranged by size:
7328 458 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2901 3185 3651 3836 3890 3909
Size: 399 241 870 284 466 185 54 19
Cuts at: 3909 4052 4085 4246 4259 4295 4313 4328 4355
Size: 143 33 161 13 36 18 15 27
Cuts at: 4355 4401 4428 4542 4643 5198 5940 6025 7096
Size: 46 27 114 101 555 742 85 1071
Cuts at: 7096 1391
Size: 2174
Fragments arranged by size:
2174 1071 870 742 555 466 399 284
241 185 161 143 114 101 85 54
46 36 33 27 27 19 18 15
13
BspEI T'CCGG_A
Cuts at: 7542 7542
Size: 7879
BspHI T'CATG_A
Cuts at: 5768 7498 5768
Size: 1730 6149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2340 2715 3285 3322 3372 3402 3606 3834
Size: 1496 375 570 37 50 30 204 228
Cuts at: 3834 3954 4131 4813 5234 844
Size: 120 177 682 421 3489
Fragments arranged by size:
3489 1496 682 570 421 375 228 204
177 120 50 37 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3562 3576 4305 5137 5766 1394
Size: 429 1739 14 729 832 629 3507
Fragments arranged by size:
3507 1739 832 729 629 429 14
BsrDI GCAATG_nn'
Cuts at: 65 1248 4464 6374 6548 65
Size: 1183 3216 1910 174 1396
Fragments arranged by size:
3216 1910 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2471 3519 4092 4790 6523 6710 1700
Size: 110 661 1048 573 698 1733 187 2869
Fragments arranged by size:
2869 1733 1048 698 661 573 187 110
BsrGI T'GTAC_A
Cuts at: 96 2580 4848 96
Size: 2484 2268 3127
Fragments arranged by size:
3127 2484 2268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7879
BssSI C'ACGA_G
Cuts at: 5939 6951 5939
Size: 1012 6867
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2341 2992 3297 4800 4828 4984 5080 5127
Size: 1547 651 305 1503 28 156 96 47
Cuts at: 5127 794
Size: 3546
Fragments arranged by size:
3546 1547 1503 651 305 156 96 47
28
BstBI TT'CG_AA
Cuts at: 2007 6683 2007
Size: 4676 3203
BstEII G'GTnAC_C
Cuts at: 1184 3119 1184
Size: 1935 5944
BstXI CCAn_nnnn'nTGG
Cuts at: 2584 3433 2584
Size: 849 7030
BstYI r'GATC_y
Cuts at: 1972 4548 4782 5209 5576 5961 5978 7430 7516
Size: 2576 234 427 367 385 17 1452 86
Cuts at: 7516 7528 7874 1972
Size: 12 346 1977
Fragments arranged by size:
2576 1977 1452 427 385 367 346 234
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3265 4515 3265
Size: 1250 6629
BtgI C'CryG_G
Cuts at: 513 1065 2022 2035 2052 2092 3214 3744 3802
Size: 552 957 13 17 40 1122 530 58
Cuts at: 3802 4033 4194 4287 513
Size: 231 161 93 4105
Fragments arranged by size:
4105 1122 957 552 530 231 161 93
58 40 17 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7140
Fragments arranged by size:
7140 683 56
BtsI GCAGTG_nn'
Cuts at: 3181 4944 5395 6189 6209 3181
Size: 1763 451 794 20 4851
Fragments arranged by size:
4851 1763 794 451 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7844
DraI TTT'AAA
Cuts at: 5183 5183
Size: 7879
DraIII CAC_nnn'GTG
Cuts at: 4390 4975 5118 4390
Size: 585 143 7151
Fragments arranged by size:
7151 585 143
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3092 3713 6886 809
Size: 507 1776 621 3173 1802
Fragments arranged by size:
3173 1802 1776 621 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2089 2322
Size: 54 1425 50 275 18 202 57 233
Cuts at: 2322 2473 2556 3217 3492 3563 4061 4090 4671
Size: 151 83 661 275 71 498 29 581
Cuts at: 4671 4740 5326 6215 7844 8
Size: 69 586 889 1629 43
Fragments arranged by size:
1629 1425 889 661 586 581 498 275
275 233 202 151 83 71 69 57
54 50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2032 2473 3217 5326 1537
Size: 275 220 441 744 2109 4090
Fragments arranged by size:
4090 2109 744 441 275 220
EarI CTCTTCn'nnn_
Cuts at: 1353 2378 3076 4378 5809 1353
Size: 1025 698 1302 1431 3423
Fragments arranged by size:
3423 1431 1302 1025 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2642 3710 6466 6840 6986 387
Size: 1064 138 1053 1068 2756 374 146 1280
Fragments arranged by size:
2756 1280 1068 1064 1053 374 146 138
EcoICRI GAG'CTC
Cuts at: 3183 4426 3183
Size: 1243 6636
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2522 2917 3271 3592 4419 4499
Size: 393 611 279 395 354 321 827 80
Cuts at: 4499 4616 4631 4754 4948 5084 5942 6539 7326
Size: 117 15 123 194 136 858 597 787
Cuts at: 7326 7594 1239
Size: 268 1524
Fragments arranged by size:
1524 858 827 787 611 597 395 393
354 321 279 268 194 136 123 117
80 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2872 3627 4016 4434 4514 2872
Size: 755 389 418 80 6237
Fragments arranged by size:
6237 755 418 389 80
EcoO109I rG'GnC_Cy
Cuts at: 2080 2317 2704 3618 4025 4049 4072 4081 4082
Size: 237 387 914 407 24 23 9 1
Cuts at: 4082 4324 4325 4352 4398 4411 4501 4639 4640
Size: 242 1 27 46 13 90 138 1
Cuts at: 4640 4876 5050 2080
Size: 236 174 4909
Fragments arranged by size:
4909 914 407 387 242 237 236 174
138 90 46 27 24 23 13 9
1 1 1
EcoRI G'AATT_C
Cuts at: 5188 5566 5188
Size: 378 7501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 4102 2698
Size: 1404 6475
FseI GG_CCGG'CC
Cuts at: 3523 3523
Size: 7879
FspI TGC'GCA
Cuts at: 2345 4832 6385 2345
Size: 2487 1553 3839
Fragments arranged by size:
3839 2487 1553
FspAI rTGC'GCAy
Cuts at: 2345 2345
Size: 7879
HaeII r_GCGC'y
Cuts at: 1678 2100 2397 2681 3140 3306 3693 4623 4900
Size: 422 297 284 459 166 387 930 277
Cuts at: 4900 5153 7026 1678
Size: 253 1873 2531
Fragments arranged by size:
2531 1873 930 459 422 387 297 284
277 253 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3214 3246 3973 4005 4736
Size: 32 135 32 1642 32 727 32 731
Cuts at: 4736 4768 6520 6552 6594 6626 1373
Size: 32 1752 32 42 32 2626
Fragments arranged by size:
2626 1752 1642 731 727 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5223 5469 669
Size: 988 281 3285 246 3079
Fragments arranged by size:
3285 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7879
HpaI GTT'AAC
Cuts at: 5469 5469
Size: 7879
KasI G'GCGC_C
Cuts at: 2096 2393 2677 3136 3302 2096
Size: 297 284 459 166 6673
Fragments arranged by size:
6673 459 297 284 166
KpnI G_GTAC'C
Cuts at: 2536 5204 2536
Size: 2668 5211
MfeI C'AATT_G
Cuts at: 5478 5478
Size: 7879
MluI A'CGCG_T
Cuts at: 7755 7755
Size: 7879
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 3526 6993 7177 1305
Size: 1182 1039 3467 184 2007
Fragments arranged by size:
3467 2007 1182 1039 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3494 10
Size: 54 1425 343 1662 4395
Fragments arranged by size:
4395 1662 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 3332 4337 5837 6196 6355 518
Size: 648 1416 750 1005 1500 359 159 2042
Fragments arranged by size:
2042 1500 1416 1005 750 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2773 2782 2992 3026 4214 4349 4654
Size: 431 736 9 210 34 1188 135 305
Cuts at: 4654 4720 4918 5295 5972 7120 7365 1606
Size: 66 198 377 677 1148 245 2120
Fragments arranged by size:
2120 1188 1148 736 677 431 377 305
245 210 198 135 66 34 9
NaeI GCC'GGC
Cuts at: 2473 3521 4792 2473
Size: 1048 1271 5560
Fragments arranged by size:
5560 1271 1048
NarI GG'CG_CC
Cuts at: 2097 2394 2678 3137 3303 2097
Size: 297 284 459 166 6673
Fragments arranged by size:
6673 459 297 284 166
NcoI C'CATG_G
Cuts at: 513 1065 2022 4033 4194 513
Size: 552 957 2011 161 4198
Fragments arranged by size:
4198 2011 957 552 161
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7879
NgoMIV G'CCGG_C
Cuts at: 2471 3519 4790 2471
Size: 1048 1271 5560
Fragments arranged by size:
5560 1271 1048
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7879
NotI GC'GGCC_GC
Cuts at: 5326 5326
Size: 7879
NspI r_CATG'y
Cuts at: 1132 1229 5083 5237 6782 1132
Size: 97 3854 154 1545 2229
Fragments arranged by size:
3854 2229 1545 154 97
PasI CC'CwG_GG
Cuts at: 1348 2974 3433 3754 4321 1348
Size: 1626 459 321 567 4906
Fragments arranged by size:
4906 1626 567 459 321
PciI A'CATG_T
Cuts at: 6778 6778
Size: 7879
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2272 1108
Size: 1164 6715
PfoI T'CCnGG_A
Cuts at: 2265 4435 4593 4905 4923 5061 2265
Size: 2170 158 312 18 138 5083
Fragments arranged by size:
5083 2170 312 158 138 18
PmeI GTTT'AAAC
Cuts at: 5183 5183
Size: 7879
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5948 5980 7461 7493 5948
Size: 32 1481 32 6334
Fragments arranged by size:
6334 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2317 2704 3618 4025 4072 2080
Size: 237 387 914 407 47 5887
Fragments arranged by size:
5887 914 407 387 237 47
PshAI GACnn'nnGTC
Cuts at: 1613 2738 1613
Size: 1125 6754
PsiI TTA'TAA
Cuts at: 5449 5449
Size: 7879
PspOMI G'GGCC_C
Cuts at: 4048 4081 4324 4351 4397 4639 4048
Size: 33 243 27 46 242 7288
Fragments arranged by size:
7288 243 242 46 33 27
PstI C_TGCA'G
Cuts at: 830 2382 2997 3248 3369 3813 3861 4185 4406
Size: 1552 615 251 121 444 48 324 221
Cuts at: 4406 4451 4659 4896 5076 5231 830
Size: 45 208 237 180 155 3478
Fragments arranged by size:
3478 1552 615 444 324 251 237 221
208 180 155 121 48 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7879
PvuII CAG'CTG
Cuts at: 2782 2992 4214 4654 4720 2782
Size: 210 1222 440 66 5941
Fragments arranged by size:
5941 1222 440 210 66
SacI G_AGCT'C
Cuts at: 3185 4428 3185
Size: 1243 6636
SacII CC_GC'GG
Cuts at: 2038 2038
Size: 7879
SalI G'TCGA_C
Cuts at: 1936 5221 1936
Size: 3285 4594
SanDI GG'GwC_CC
Cuts at: 4025 4025
Size: 7879
SapI GCTCTTCn'nnn_
Cuts at: 2378 4378 2378
Size: 2000 5879
SbfI CC_TGCA'GG
Cuts at: 4185 5231 4185
Size: 1046 6833
ScaI AGT'ACT
Cuts at: 2303 6127 7839 2303
Size: 3824 1712 2343
Fragments arranged by size:
3824 2343 1712
SexAI A'CCwGG_T
Cuts at: 2289 3123 4386 2289
Size: 834 1263 5782
Fragments arranged by size:
5782 1263 834
SfcI C'TryA_G
Cuts at: 826 1045 2378 2993 3244 3365 3809 3857 4181
Size: 219 1333 615 251 121 444 48 324
Cuts at: 4181 4402 4447 4655 4892 5072 5227 6362 7043
Size: 221 45 208 237 180 155 1135 681
Cuts at: 7043 7234 826
Size: 191 1471
Fragments arranged by size:
1471 1333 1135 681 615 444 324 251
237 221 219 208 191 180 155 121
48 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 3276 4089 3276
Size: 813 7066
SfoI GGC'GCC
Cuts at: 2098 2395 2679 3138 3304 2098
Size: 297 284 459 166 6673
Fragments arranged by size:
6673 459 297 284 166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7879
SmaI CCC'GGG
Cuts at: 1872 2756 3047 4164 5206 1872
Size: 884 291 1117 1042 4545
Fragments arranged by size:
4545 1117 1042 884 291
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3540 4130 5983 6884 7146 820
Size: 197 926 1597 590 1853 901 262 1553
Fragments arranged by size:
1853 1597 1553 926 901 590 262 197
SnaBI TAC'GTA
Cuts at: 493 5176 5670 493
Size: 4683 494 2702
Fragments arranged by size:
4683 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7879
SphI G_CATG'C
Cuts at: 1132 5237 1132
Size: 4105 3774
SspI AAT'ATT
Cuts at: 5 52 5803 5
Size: 47 5751 2081
Fragments arranged by size:
5751 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7879
StyI C'CwwG_G
Cuts at: 513 1065 2022 3897 4033 4077 4194 5318 513
Size: 552 957 1875 136 44 117 1124 3074
Fragments arranged by size:
3074 1875 1124 957 552 136 117 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2860 3384 3388 3392 3500 3853 4251
Size: 1255 1280 524 4 4 108 353 398
Cuts at: 4251 5906 5923 6076 6261 6706 325
Size: 1655 17 153 185 445 1498
Fragments arranged by size:
1655 1498 1280 1255 524 445 398 353
185 153 108 17 4 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 2580 4848
Size: 274 80 33 51 1443 324 279 2268
Cuts at: 4848 6125 7837 96
Size: 1277 1712 138
Fragments arranged by size:
2268 1712 1443 1277 324 279 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2945 2977 3520 3552 7839 7871 2945
Size: 32 543 32 4287 32 2953
Fragments arranged by size:
4287 2953 543 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3092 1316
Size: 1776 6103
XbaI T'CTAG_A
Cuts at: 5215 5215
Size: 7879
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2953 3476 3751 4391 2953
Size: 523 275 640 6441
Fragments arranged by size:
6441 640 523 275
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7879
XmaI C'CCGG_G
Cuts at: 1870 2754 3045 4162 5204 1870
Size: 884 291 1117 1042 4545
Fragments arranged by size:
4545 1117 1042 884 291
XmnI GAAnn'nnTTC
Cuts at: 6008 6008
Size: 7879
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5687 276
Size: 53 83 186 936 4153 2468
Fragments arranged by size:
4153 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I
EcoRV NruI NsiI PacI PmlI PspXI PsrI RsrII
SrfI SwaI