Restriction map for
pFN21ASDA0719
(Circular) (Six-base) MAPSORT of: pFN21ASDA0719.seq Check: 5480 from: 1 to: 6553
pFN21ASDA0719 6553 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4363 278
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3896 1937
Size: 1959 4594
Acc65I G'GTAC_C
Cuts at: 3874 3874
Size: 6553
AclI AA'CG_TT
Cuts at: 1563 4680 5053 1563
Size: 3117 373 3063
Fragments arranged by size:
3117 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 2340 2788 3012 3569 4616 6000 6268
Size: 641 67 448 224 557 1047 1384 268
Cuts at: 6268 1632
Size: 1917
Fragments arranged by size:
1917 1384 1047 641 557 448 268 224
67
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6356
AflIII A'CryG_T
Cuts at: 1203 3720 5452 6429 1203
Size: 2517 1732 977 1327
Fragments arranged by size:
2517 1732 1327 977
AgeI A'CCGG_T
Cuts at: 5384 5384
Size: 6553
AhdI GACnn_n'nnGTC
Cuts at: 5282 5282
Size: 6553
AleI CACnn'nnGTG
Cuts at: 1166 2657 1166
Size: 1491 5062
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3170 1184
Size: 1986 4567
AlwNI CAG_nnn'CTG
Cuts at: 2066 4296 5868 2066
Size: 2230 1572 2751
Fragments arranged by size:
2751 2230 1572
ApaI G_GGCC'C
Cuts at: 2114 2114
Size: 6553
ApaLI G'TGCA_C
Cuts at: 4610 5766 4610
Size: 1156 5397
ApoI r'AATT_y
Cuts at: 1493 2905 3434 3862 4088 4240 6349 1493
Size: 1412 529 428 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 529 428 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5107 160
Size: 872 4075 1606
Fragments arranged by size:
4075 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
AvaI C'yCGr_G
Cuts at: 1870 1943 2113 2302 3878 1870
Size: 73 170 189 1576 4545
Fragments arranged by size:
4545 1576 189 170 73
BamHI G'GATC_C
Cuts at: 3883 4250 3883
Size: 367 6186
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2161 2183 2214 3874 5329
Size: 325 217 627 374 22 31 1660 1455
Cuts at: 5329 618
Size: 1842
Fragments arranged by size:
1842 1660 1455 627 374 325 217 31
22
BanII G_rGCy'C
Cuts at: 1391 2078 2114 2365 3872 1391
Size: 687 36 251 1507 4072
Fragments arranged by size:
4072 1507 687 251 36
BbeI G_GCGC'C
Cuts at: 2218 2218
Size: 6553
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2279 928
Size: 971 380 5202
Fragments arranged by size:
5202 971 380
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3440 3474 4744 4778 1903
Size: 34 1503 34 1270 34 3678
Fragments arranged by size:
3678 1503 1270 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2906 4445 5655 2906
Size: 1539 1210 3804
Fragments arranged by size:
3804 1539 1210
BclI T'GATC_A
Cuts at: 1548 3265 1548
Size: 1717 4836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2137 2297 5164
Size: 107 122 71 631 605 465 160 2867
Cuts at: 5164 136
Size: 1525
Fragments arranged by size:
2867 1525 631 605 465 160 122 107
71
BglII A'GATC_T
Cuts at: 3598 6548 3598
Size: 2950 3603
BlpI GC'TnA_GC
Cuts at: 3970 3970
Size: 6553
Bme1580I G_kGCm'C
Cuts at: 1790 2114 2120 2164 2456 4614 5770 1790
Size: 324 6 44 292 2158 1156 2573
Fragments arranged by size:
2573 2158 1156 324 292 44 6
BmgBI CAC'GTC
Cuts at: 1318 2600 1318
Size: 1282 5271
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4284 5242 442
Size: 416 53 83 399 24 2867 958 1753
Fragments arranged by size:
2867 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5213 1239
Size: 3974 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3168 4678 5543 5841 3168
Size: 1510 865 298 3880
Fragments arranged by size:
3880 1510 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5216 882
Size: 617 3717 2219
Fragments arranged by size:
3717 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3850 4344 493
Size: 711 2646 494 2702
Fragments arranged by size:
2702 2646 711 494
BsaBI GATnn'nnATC
Cuts at: 3231 6547 3231
Size: 3316 3237
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2215 2241 4360 4742
Size: 53 83 186 936 682 26 2119 382
Cuts at: 4742 5377 275
Size: 635 1451
Fragments arranged by size:
2119 1451 936 682 635 382 186 83
53 26
BsaWI w'CCGG_w
Cuts at: 4986 5384 5658 5805 6216 4986
Size: 398 274 147 411 5323
Fragments arranged by size:
5323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6328
Fragments arranged by size:
6328 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6553
BseYI C'CCAG_C
Cuts at: 2173 2832 5756 6252 2173
Size: 659 2924 496 2474
Fragments arranged by size:
2924 2474 659 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5980
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2157 4003 4764 5792 1540
Size: 275 186 156 1846 761 1028 2301
Fragments arranged by size:
2301 1846 1028 761 275 186 156
BsiHKAI G_wGCw'C
Cuts at: 2078 2335 4614 4699 5770 2078
Size: 257 2279 85 1071 2861
Fragments arranged by size:
2861 2279 1071 257 85
BsmI GAATG_Cn'
Cuts at: 2975 3226 4064 4157 2975
Size: 251 838 93 5371
Fragments arranged by size:
5371 838 251 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2114 2120 2164 2335 2365 2456
Size: 399 288 36 6 44 171 30 91
Cuts at: 2456 3872 4614 4699 5770 1391
Size: 1416 742 85 1071 2174
Fragments arranged by size:
2174 1416 1071 742 399 288 171 91
85 44 36 30 6
BspEI T'CCGG_A
Cuts at: 6216 6216
Size: 6553
BspHI T'CATG_A
Cuts at: 4442 6172 4442
Size: 1730 4823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3048 3908 844
Size: 2204 860 3489
Fragments arranged by size:
3489 2204 860
BsrBI CCG'CTC
Cuts at: 1394 1823 4440 1394
Size: 429 2617 3507
Fragments arranged by size:
3507 2617 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3014 3058 5048 5222 65
Size: 1183 1766 44 1990 174 1396
Fragments arranged by size:
1990 1766 1396 1183 174 44
BsrFI r'CCGG_y
Cuts at: 1700 1810 5197 5384 1700
Size: 110 3387 187 2869
Fragments arranged by size:
3387 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2549 3150 96
Size: 2453 601 3499
Fragments arranged by size:
3499 2453 601
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6553
BssSI C'ACGA_G
Cuts at: 4613 5625 4613
Size: 1012 5541
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3172 794
Size: 2378 4175
BstBI TT'CG_AA
Cuts at: 2007 5357 2007
Size: 3350 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6553
BstYI r'GATC_y
Cuts at: 1972 3598 3883 4250 4635 4652 6104 6190 6202
Size: 1626 285 367 385 17 1452 86 12
Cuts at: 6202 6548 1972
Size: 346 1977
Fragments arranged by size:
1977 1626 1452 385 367 346 285 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3672 513
Size: 552 957 1650 3394
Fragments arranged by size:
3394 1650 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2149 3801 487
Size: 683 56 923 1652 3239
Fragments arranged by size:
3239 1652 923 683 56
BtsI GCAGTG_nn'
Cuts at: 2652 3288 4069 4863 4883 2652
Size: 636 781 794 20 4322
Fragments arranged by size:
4322 794 781 636 20
ClaI AT'CG_AT
Cuts at: 1554 3210 1554
Size: 1656 4897
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3569 3604 529
Size: 35 3005 35 3478
Fragments arranged by size:
3478 3005 35 35
DraI TTT'AAA
Cuts at: 2398 3857 2398
Size: 1459 5094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5560 809
Size: 507 4244 1802
Fragments arranged by size:
4244 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 3744 4000
Size: 54 1425 50 275 18 195 1719 256
Cuts at: 4000 4889 6518 8
Size: 889 1629 43
Fragments arranged by size:
1719 1629 1425 889 275 256 195 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4000 1537
Size: 275 2188 4090
Fragments arranged by size:
4090 2188 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3391 3414 4483 1353
Size: 2038 23 1069 3423
Fragments arranged by size:
3423 2038 1069 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5140 5514 5660 387
Size: 1064 138 3551 374 146 1280
Fragments arranged by size:
3551 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2076 2076
Size: 6553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2340 2788 3012 3569 4616 5213
Size: 393 641 67 448 224 557 1047 597
Cuts at: 5213 6000 6268 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1047 787 641 597 557 448 393
268 224 67
EcoO109I rG'GnC_Cy
Cuts at: 2324 2324
Size: 6553
EcoRI G'AATT_C
Cuts at: 3862 4240 3862
Size: 378 6175
EcoRV GAT'ATC
Cuts at: 2824 2824
Size: 6553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3593 3593
Size: 6553
FspI TGC'GCA
Cuts at: 5059 5059
Size: 6553
HaeII r_GCGC'y
Cuts at: 1678 2218 5700 1678
Size: 540 3482 2531
Fragments arranged by size:
3482 2531 540
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2333 2365 2764 2796 3754
Size: 32 135 32 761 32 399 32 958
Cuts at: 3754 3786 5194 5226 5268 5300 1373
Size: 32 1408 32 42 32 2626
Fragments arranged by size:
2626 1408 958 761 399 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2473 3897 4143 669
Size: 988 281 535 1424 246 3079
Fragments arranged by size:
3079 1424 988 535 281 246
HindIII A'AGCT_T
Cuts at: 748 3113 748
Size: 2365 4188
HpaI GTT'AAC
Cuts at: 4143 4143
Size: 6553
KasI G'GCGC_C
Cuts at: 2214 2214
Size: 6553
KpnI G_GTAC'C
Cuts at: 3878 3878
Size: 6553
MfeI C'AATT_G
Cuts at: 4152 4152
Size: 6553
MluI A'CGCG_T
Cuts at: 6429 6429
Size: 6553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5667 5851 1305
Size: 4362 184 2007
Fragments arranged by size:
4362 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3746 10
Size: 54 1425 343 1914 2817
Fragments arranged by size:
2817 1914 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2657 3152 3343 3719 4511 4870 5029
Size: 648 1491 495 191 376 792 359 159
Cuts at: 5029 518
Size: 2042
Fragments arranged by size:
2042 1491 792 648 495 376 359 191
159
MspA1I CmG'CkG
Cuts at: 1606 2051 2066 2143 2247 2310 3250 3969 4646
Size: 445 15 77 104 63 940 719 677
Cuts at: 4646 5794 6039 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 940 719 677 445 245 104
77 63 15
NarI GG'CG_CC
Cuts at: 2215 2215
Size: 6553
NcoI C'CATG_G
Cuts at: 513 1065 2022 3672 513
Size: 552 957 1650 3394
Fragments arranged by size:
3394 1650 957 552
NdeI CA'TA_TG
Cuts at: 387 3559 387
Size: 3172 3381
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6553
NotI GC'GGCC_GC
Cuts at: 4000 4000
Size: 6553
NspI r_CATG'y
Cuts at: 1132 1229 2698 3151 3157 3724 3911 5456 1132
Size: 97 1469 453 6 567 187 1545 2229
Fragments arranged by size:
2229 1545 1469 567 453 187 97 6
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6553
PciI A'CATG_T
Cuts at: 3720 5452 3720
Size: 1732 4821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6553
PfoI T'CCnGG_A
Cuts at: 2078 2078
Size: 6553
PmeI GTTT'AAAC
Cuts at: 3857 3857
Size: 6553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4622 4654 6135 6167 4622
Size: 32 1481 32 5008
Fragments arranged by size:
5008 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2324 2324
Size: 6553
PshAI GACnn'nnGTC
Cuts at: 1613 3319 1613
Size: 1706 4847
PsiI TTA'TAA
Cuts at: 4123 4123
Size: 6553
PspOMI G'GGCC_C
Cuts at: 2110 2110
Size: 6553
PstI C_TGCA'G
Cuts at: 830 3905 830
Size: 3075 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6553
PvuII CAG'CTG
Cuts at: 2143 3250 2143
Size: 1107 5446
SacI G_AGCT'C
Cuts at: 2078 2078
Size: 6553
SalI G'TCGA_C
Cuts at: 1936 3895 1936
Size: 1959 4594
SbfI CC_TGCA'GG
Cuts at: 3905 3905
Size: 6553
ScaI AGT'ACT
Cuts at: 4801 6513 4801
Size: 1712 4841
SfcI C'TryA_G
Cuts at: 826 1045 2457 3901 5036 5717 5908 826
Size: 219 1412 1444 1135 681 191 1471
Fragments arranged by size:
1471 1444 1412 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2297 2297
Size: 6553
SfoI GGC'GCC
Cuts at: 2216 2216
Size: 6553
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6553
SmaI CCC'GGG
Cuts at: 1872 2115 2304 3880 1872
Size: 243 189 1576 4545
Fragments arranged by size:
4545 1576 243 189
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3183 4657 5558 5820 820
Size: 197 926 1240 1474 901 262 1553
Fragments arranged by size:
1553 1474 1240 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3850 4344 493
Size: 3357 494 2702
Fragments arranged by size:
3357 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6553
SphI G_CATG'C
Cuts at: 1132 2698 3911 1132
Size: 1566 1213 3774
Fragments arranged by size:
3774 1566 1213
SrfI GCCC'GGGC
Cuts at: 2115 2115
Size: 6553
SspI AAT'ATT
Cuts at: 5 52 2393 4477 5
Size: 47 2341 2084 2081
Fragments arranged by size:
2341 2084 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6553
StyI C'CwwG_G
Cuts at: 513 1065 2022 2725 3440 3672 3992 513
Size: 552 957 703 715 232 320 3074
Fragments arranged by size:
3074 957 715 703 552 320 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4580 4597 4750 4935 5380 325
Size: 1255 3000 17 153 185 445 1498
Fragments arranged by size:
3000 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2549 3150 3688
Size: 274 80 33 51 1443 572 601 538
Cuts at: 3688 4799 6511 96
Size: 1111 1712 138
Fragments arranged by size:
1712 1443 1111 601 572 538 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6513 6545 6513
Size: 32 6521
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6553
XbaI T'CTAG_A
Cuts at: 3889 3889
Size: 6553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2932 3754 2932
Size: 822 5731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6553
XmaI C'CCGG_G
Cuts at: 1870 2113 2302 3878 1870
Size: 243 189 1576 4545
Fragments arranged by size:
4545 1576 243 189
XmnI GAAnn'nnTTC
Cuts at: 4682 4682
Size: 6553
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4361 276
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SwaI