Restriction map for
pFN21ASDA0696
(Circular) (Six-base) MAPSORT of: pFN21ASDA0696.seq Check: 7579 from: 1 to: 6355
pFN21ASDA0696 6355 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4165 278
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2751 3129 3698 1937
Size: 814 378 569 4594
Fragments arranged by size:
4594 814 569 378
Acc65I G'GTAC_C
Cuts at: 3676 3676
Size: 6355
AclI AA'CG_TT
Cuts at: 1563 4482 4855 1563
Size: 2919 373 3063
Fragments arranged by size:
3063 2919 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2375 2898 2989 4418 5802 6070 1632
Size: 743 523 91 1429 1384 268 1917
Fragments arranged by size:
1917 1429 1384 743 523 268 91
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6158
AflIII A'CryG_T
Cuts at: 1203 3502 5254 6231 1203
Size: 2299 1752 977 1327
Fragments arranged by size:
2299 1752 1327 977
AgeI A'CCGG_T
Cuts at: 5186 5186
Size: 6355
AhdI GACnn_n'nnGTC
Cuts at: 2269 5084 2269
Size: 2815 3540
AleI CACnn'nnGTG
Cuts at: 1166 2317 3485 1166
Size: 1151 1168 4036
Fragments arranged by size:
4036 1168 1151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2149 1184
Size: 965 5390
AlwNI CAG_nnn'CTG
Cuts at: 2593 2867 4098 5670 2593
Size: 274 1231 1572 3278
Fragments arranged by size:
3278 1572 1231 274
ApaLI G'TGCA_C
Cuts at: 4412 5568 4412
Size: 1156 5199
ApoI r'AATT_y
Cuts at: 1493 2291 2626 3203 3664 3890 4042 6151 1493
Size: 798 335 577 461 226 152 2109 1697
Fragments arranged by size:
2109 1697 798 577 461 335 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 3450 3680 1870
Size: 73 1507 230 4545
Fragments arranged by size:
4545 1507 230 73
BamHI G'GATC_C
Cuts at: 3685 4052 3685
Size: 367 5988
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3320 3676 5131 618
Size: 325 217 627 1533 356 1455 1842
Fragments arranged by size:
1842 1533 1455 627 356 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 2064 3457 3674 1391
Size: 640 33 1393 217 4072
Fragments arranged by size:
4072 1393 640 217 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5384
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4546 4580 1903
Size: 34 2609 34 3678
Fragments arranged by size:
3678 2609 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4247 5457 4247
Size: 1210 5145
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6355
BfrBI ATG'CAT
Cuts at: 3366 3366
Size: 6355
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4966 136
Size: 107 122 71 631 605 3294 1525
Fragments arranged by size:
3294 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6350 6350
Size: 6355
BlpI GC'TnA_GC
Cuts at: 3772 3772
Size: 6355
Bme1580I G_kGCm'C
Cuts at: 1790 3228 3601 4416 5572 1790
Size: 1438 373 815 1156 2573
Fragments arranged by size:
2573 1438 1156 815 373
BmgBI CAC'GTC
Cuts at: 1318 3503 1318
Size: 2185 4170
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2145 3588 4086
Size: 416 53 83 399 24 728 1443 498
Cuts at: 4086 5044 442
Size: 958 1753
Fragments arranged by size:
1753 1443 958 728 498 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 6355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2185 3205 3233 3500 3607 5015 1239
Size: 911 35 1020 28 267 107 1408 2579
Fragments arranged by size:
2579 1408 1020 911 267 107 35 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4480 5345 5643 4480
Size: 865 298 5192
Fragments arranged by size:
5192 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2454 3602 5018 882
Size: 617 955 1148 1416 2219
Fragments arranged by size:
2219 1416 1148 955 617
BsaAI yAC'GTr
Cuts at: 493 1204 3652 4146 493
Size: 711 2448 494 2702
Fragments arranged by size:
2702 2448 711 494
BsaBI GATnn'nnATC
Cuts at: 6349 6349
Size: 6355
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4162 4544 5179 275
Size: 53 83 186 936 2629 382 635 1451
Fragments arranged by size:
2629 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3369 4788 5186 5460 5607 6018 3369
Size: 1419 398 274 147 411 3706
Fragments arranged by size:
3706 1419 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3601 3631 1178
Size: 30 165 30 2198 30 3902
Fragments arranged by size:
3902 2198 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6355
BseYI C'CCAG_C
Cuts at: 3457 5558 6054 3457
Size: 2101 496 3758
Fragments arranged by size:
3758 2101 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2478 1163
Size: 573 742 5040
Fragments arranged by size:
5040 742 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3075 3805 4566 5594 1540
Size: 275 186 1074 730 761 1028 2301
Fragments arranged by size:
2301 1074 1028 761 730 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 3191 3197 4416 4501 5572 2064
Size: 1127 6 1219 85 1071 2847
Fragments arranged by size:
2847 1219 1127 1071 85 6
BsmI GAATG_Cn'
Cuts at: 2511 3866 3959 2511
Size: 1355 93 4907
Fragments arranged by size:
4907 1355 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2203 2203
Size: 6355
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2064 3191 3197 3228 3457 3601
Size: 399 241 33 1127 6 31 229 144
Cuts at: 3601 3674 4416 4501 5572 1391
Size: 73 742 85 1071 2174
Fragments arranged by size:
2174 1127 1071 742 399 241 229 144
85 73 33 31 6
BspEI T'CCGG_A
Cuts at: 6018 6018
Size: 6355
BspHI T'CATG_A
Cuts at: 4244 5974 4244
Size: 1730 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3710 844
Size: 2866 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2730 3042 4242 1394
Size: 429 907 312 1200 3507
Fragments arranged by size:
3507 1200 907 429 312
BsrDI GCAATG_nn'
Cuts at: 65 1248 3016 3045 3237 4850 5024 65
Size: 1183 1768 29 192 1613 174 1396
Fragments arranged by size:
1768 1613 1396 1183 192 174 29
BsrFI r'CCGG_y
Cuts at: 1700 1810 4999 5186 1700
Size: 110 3189 187 2869
Fragments arranged by size:
3189 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6355
BssSI C'ACGA_G
Cuts at: 3190 4415 5427 3190
Size: 1225 1012 4118
Fragments arranged by size:
4118 1225 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2146 794
Size: 1352 5003
BstBI TT'CG_AA
Cuts at: 2007 5159 2007
Size: 3152 3203
BstEII G'GTnAC_C
Cuts at: 1184 3022 1184
Size: 1838 4517
BstYI r'GATC_y
Cuts at: 1972 3685 4052 4437 4454 5906 5992 6004 6350
Size: 1713 367 385 17 1452 86 12 346
Cuts at: 6350 1972
Size: 1977
Fragments arranged by size:
1977 1713 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4846
Fragments arranged by size:
4846 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5616
Fragments arranged by size:
5616 683 56
BtsI GCAGTG_nn'
Cuts at: 3871 4665 4685 3871
Size: 794 20 5541
Fragments arranged by size:
5541 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6320
DraI TTT'AAA
Cuts at: 2599 3659 2599
Size: 1060 5295
DraIII CAC_nnn'GTG
Cuts at: 3471 3471
Size: 6355
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5362 809
Size: 507 4046 1802
Fragments arranged by size:
4046 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3103 3802 4691
Size: 54 1425 50 275 18 1273 699 889
Cuts at: 4691 6320 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1273 889 699 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3802 1537
Size: 275 1990 4090
Fragments arranged by size:
4090 1990 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2569 2917 4285 1353
Size: 1216 348 1368 3423
Fragments arranged by size:
3423 1368 1216 348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4942 5316 5462 387
Size: 1064 138 3353 374 146 1280
Fragments arranged by size:
3353 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2062 2062
Size: 6355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2185 2375 2898 2989 3205 3233
Size: 393 518 35 190 523 91 216 28
Cuts at: 3233 3500 3607 4418 5015 5802 6070 1239
Size: 267 107 811 597 787 268 1524
Fragments arranged by size:
1524 811 787 597 523 518 393 268
267 216 190 107 91 35 28
EcoO109I rG'GnC_Cy
Cuts at: 3399 3399
Size: 6355
EcoRI G'AATT_C
Cuts at: 3664 4042 3664
Size: 378 5977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2525 3458 2525
Size: 933 5422
FspI TGC'GCA
Cuts at: 2125 3479 4861 2125
Size: 1354 1382 3619
Fragments arranged by size:
3619 1382 1354
FspAI rTGC'GCAy
Cuts at: 2125 2125
Size: 6355
HaeII r_GCGC'y
Cuts at: 1678 5502 1678
Size: 3824 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2432 2464 3601
Size: 32 135 32 683 32 145 32 1137
Cuts at: 3601 3633 4996 5028 5070 5102 1373
Size: 32 1363 32 42 32 2626
Fragments arranged by size:
2626 1363 1137 683 145 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2850 3699 3945 669
Size: 988 281 912 849 246 3079
Fragments arranged by size:
3079 988 912 849 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6355
HpaI GTT'AAC
Cuts at: 2850 3945 2850
Size: 1095 5260
KpnI G_GTAC'C
Cuts at: 3680 3680
Size: 6355
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5469 5653 1305
Size: 4164 184 2007
Fragments arranged by size:
4164 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3105 10
Size: 54 1425 343 1273 3260
Fragments arranged by size:
3260 1425 1273 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2317 2979 3050 3485 4313 4672 4831
Size: 648 1151 662 71 435 828 359 159
Cuts at: 4831 518
Size: 2042
Fragments arranged by size:
2042 1151 828 662 648 435 359 159
71
MspA1I CmG'CkG
Cuts at: 1606 2371 3771 4448 5596 5841 1606
Size: 765 1400 677 1148 245 2120
Fragments arranged by size:
2120 1400 1148 765 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4846
Fragments arranged by size:
4846 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6355
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NsiI A_TGCA'T
Cuts at: 3368 3368
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 2122 2149 3713 5258 1132
Size: 97 893 27 1564 1545 2229
Fragments arranged by size:
2229 1564 1545 893 97 27
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6355
PciI A'CATG_T
Cuts at: 5254 5254
Size: 6355
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6355
PfoI T'CCnGG_A
Cuts at: 2661 2661
Size: 6355
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 5937 5969 4424
Size: 32 1481 32 4810
Fragments arranged by size:
4810 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6355
PsiI TTA'TAA
Cuts at: 3925 3925
Size: 6355
PspXI vC'TCGA_Gb
Cuts at: 3450 3450
Size: 6355
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2520 2552 2520
Size: 32 6323
PstI C_TGCA'G
Cuts at: 830 2142 2883 3707 830
Size: 1312 741 824 3478
Fragments arranged by size:
3478 1312 824 741
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
SacI G_AGCT'C
Cuts at: 2064 2064
Size: 6355
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SapI GCTCTTCn'nnn_
Cuts at: 2917 2917
Size: 6355
SbfI CC_TGCA'GG
Cuts at: 3707 3707
Size: 6355
ScaI AGT'ACT
Cuts at: 4603 6315 4603
Size: 1712 4643
SexAI A'CCwGG_T
Cuts at: 2106 2106
Size: 6355
SfcI C'TryA_G
Cuts at: 826 1045 2138 2676 2879 3703 4838 5519 5710
Size: 219 1093 538 203 824 1135 681 191
Cuts at: 5710 826
Size: 1471
Fragments arranged by size:
1471 1135 1093 824 681 538 219 203
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 3682 1872
Size: 1810 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3450 4459 5360 5622 820
Size: 197 926 1507 1009 901 262 1553
Fragments arranged by size:
1553 1507 1009 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3652 4146 493
Size: 3159 494 2702
Fragments arranged by size:
3159 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 2149 3713 1132
Size: 1017 1564 3774
Fragments arranged by size:
3774 1564 1017
SspI AAT'ATT
Cuts at: 5 52 2258 4279 5
Size: 47 2206 2021 2081
Fragments arranged by size:
2206 2081 2021 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6355
StyI C'CwwG_G
Cuts at: 513 1065 2022 2078 3033 3794 513
Size: 552 957 56 955 761 3074
Fragments arranged by size:
3074 957 955 761 552 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3089 4382 4399 4552 4737 5182 325
Size: 1255 1509 1293 17 153 185 445 1498
Fragments arranged by size:
1509 1498 1293 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3144 3252 4601
Size: 274 80 33 51 1443 1167 108 1349
Cuts at: 4601 6313 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1349 1167 274 138 108 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3006 3038 3379 3411 6315 6347 3006
Size: 32 341 32 2904 32 3014
Fragments arranged by size:
3014 2904 341 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6355
XbaI T'CTAG_A
Cuts at: 3644 3691 3644
Size: 47 6308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2085 2174 2085
Size: 89 6266
XhoI C'TCGA_G
Cuts at: 1943 3450 1943
Size: 1507 4848
XmaI C'CCGG_G
Cuts at: 1870 3680 1870
Size: 1810 4545
XmnI GAAnn'nnTTC
Cuts at: 2424 3207 4484 2424
Size: 783 1277 4295
Fragments arranged by size:
4295 1277 783
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4163 276
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PstI PvuI SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV
FseI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PvuII RsrII SacII SanDI SfiI SfoI
SrfI SwaI