Restriction map for
pFN21ASDA0693
(Circular) (Six-base) MAPSORT of: pFN21ASDA0693.seq Check: 1561 from: 1 to: 5917
pFN21ASDA0693 5917 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2199 3260 1937
Size: 262 1061 4594
Fragments arranged by size:
4594 1061 262
Acc65I G'GTAC_C
Cuts at: 2970 3238 2970
Size: 268 5649
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3980 5364 5632 1632
Size: 2348 1384 268 1917
Fragments arranged by size:
2348 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 2409 2541 4816 5793 1203
Size: 1206 132 2275 977 1327
Fragments arranged by size:
2275 1327 1206 977 132
AgeI A'CCGG_T
Cuts at: 2898 4748 2898
Size: 1850 4067
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2375 1184
Size: 1191 4726
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2222 2254 2222
Size: 32 5885
AlwNI CAG_nnn'CTG
Cuts at: 2182 2242 2317 2761 3660 5232 2182
Size: 60 75 444 899 1572 2867
Fragments arranged by size:
2867 1572 899 444 75 60
ApaI G_GGCC'C
Cuts at: 2282 2472 2282
Size: 190 5727
ApaLI G'TGCA_C
Cuts at: 2161 3974 5130 2161
Size: 1813 1156 2948
Fragments arranged by size:
2948 1813 1156
ApoI r'AATT_y
Cuts at: 1493 3226 3452 3604 5713 1493
Size: 1733 226 152 2109 1697
Fragments arranged by size:
2109 1733 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 3038 3242 1870
Size: 73 1095 204 4545
Fragments arranged by size:
4545 1095 204 73
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2428 2525 2719 2807 2970
Size: 325 217 627 641 97 194 88 163
Cuts at: 2970 2981 3238 4693 618
Size: 11 257 1455 1842
Fragments arranged by size:
1842 1455 641 627 325 257 217 194
163 97 88 11
BanII G_rGCy'C
Cuts at: 1391 2282 2290 2472 2820 3076 3236 1391
Size: 891 8 182 348 256 160 4072
Fragments arranged by size:
4072 891 348 256 182 160 8
BbeI G_GCGC'C
Cuts at: 2432 2723 2811 2432
Size: 291 88 5538
Fragments arranged by size:
5538 291 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5019 3809
Size: 1210 4707
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5917
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2391 2728 4528
Size: 107 122 71 631 605 719 337 1800
Cuts at: 4528 136
Size: 1525
Fragments arranged by size:
1800 1525 719 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 5912 5912
Size: 5917
BlpI GC'TnA_GC
Cuts at: 3334 3334
Size: 5917
Bme1580I G_kGCm'C
Cuts at: 1790 2165 2282 2472 3978 5134 1790
Size: 375 117 190 1506 1156 2573
Fragments arranged by size:
2573 1506 1156 375 190 117
BmgBI CAC'GTC
Cuts at: 1318 2607 1318
Size: 1289 4628
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2570 2941 3648
Size: 416 53 83 399 24 1153 371 707
Cuts at: 3648 4606 442
Size: 958 1753
Fragments arranged by size:
1753 1153 958 707 416 399 371 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2290 2304 4577 1239
Size: 911 140 14 2273 2579
Fragments arranged by size:
2579 2273 911 140 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4042 4907 5205 4042
Size: 865 298 4754
Fragments arranged by size:
4754 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4580 882
Size: 617 3081 2219
Fragments arranged by size:
3081 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2410 2542 3214 3708 493
Size: 711 1206 132 672 494 2702
Fragments arranged by size:
2702 1206 711 672 494 132
BsaBI GATnn'nnATC
Cuts at: 5911 5911
Size: 5917
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2343 2429 2505 2720
Size: 53 83 186 936 810 86 76 215
Cuts at: 2720 2808 3724 4106 4741 275
Size: 88 916 382 635 1451
Fragments arranged by size:
1451 936 916 810 635 382 215 186
88 86 83 76 53
BsaWI w'CCGG_w
Cuts at: 2187 2898 4350 4748 5022 5169 5580 2187
Size: 711 1452 398 274 147 411 2524
Fragments arranged by size:
2524 1452 711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2564 2594 2717 2747 1178
Size: 30 165 30 1161 30 123 30 4348
Fragments arranged by size:
4348 1161 165 123 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2359 2786 2924 1186
Size: 1173 427 138 4179
Fragments arranged by size:
4179 1173 427 138
BseYI C'CCAG_C
Cuts at: 2736 2751 3006 3020 3156 5120 5616 2736
Size: 15 255 14 136 1964 496 3037
Fragments arranged by size:
3037 1964 496 255 136 15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 2303 1163
Size: 573 432 135 4777
Fragments arranged by size:
4777 573 432 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2157 2214 2899 3367 4128 5156
Size: 275 186 156 57 685 468 761 1028
Cuts at: 5156 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 685 468 275 186 156
57
BsiHKAI G_wGCw'C
Cuts at: 2165 3136 3978 4063 5134 2165
Size: 971 842 85 1071 2948
Fragments arranged by size:
2948 1071 971 842 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2282 2290 2472 2820 3076 3136
Size: 399 375 117 8 182 348 256 60
Cuts at: 3136 3236 3978 4063 5134 1391
Size: 100 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 348 256 182
117 100 85 60 8
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3272 844
Size: 2428 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2301 3804 1394
Size: 429 478 1503 3507
Fragments arranged by size:
3507 1503 478 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4412 4586 65
Size: 1183 3164 174 1396
Fragments arranged by size:
3164 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2727 2898 4561 4748 1700
Size: 110 917 171 1663 187 2869
Fragments arranged by size:
2869 1663 917 187 171 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2182 2242 794
Size: 1388 60 4469
Fragments arranged by size:
4469 1388 60
BstBI TT'CG_AA
Cuts at: 2007 4721 2007
Size: 2714 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2029
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 2178 3247 3614 3999 4016 5468 5554 5566
Size: 206 1069 367 385 17 1452 86 12
Cuts at: 5566 5912 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1069 385 367 346 206 86
17 12
BstZ17I GTA'TAC
Cuts at: 2200 2200
Size: 5917
BtgI C'CryG_G
Cuts at: 513 1065 2028 2715 2811 2820 2848 513
Size: 552 963 687 96 9 28 3582
Fragments arranged by size:
3582 963 687 552 96 28 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2647 487
Size: 683 56 1421 3757
Fragments arranged by size:
3757 1421 683 56
BtsI GCAGTG_nn'
Cuts at: 3433 4227 4247 3433
Size: 794 20 5103
Fragments arranged by size:
5103 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3148 4924 809
Size: 507 1832 1776 1802
Fragments arranged by size:
1832 1802 1776 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2048 2154 2205
Size: 54 1425 50 275 18 218 106 51
Cuts at: 2205 2601 2845 3364 4253 5882 8
Size: 396 244 519 889 1629 43
Fragments arranged by size:
1629 1425 889 519 396 275 244 218
106 54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2154 3364 1537
Size: 275 342 1210 4090
Fragments arranged by size:
4090 1210 342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2752 3847 1353
Size: 1399 1095 3423
Fragments arranged by size:
3423 1399 1095
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4504 4878 5024 387
Size: 1064 138 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2290 2304 3980 4577 5364 5632
Size: 393 518 140 14 1676 597 787 268
Cuts at: 5632 1239
Size: 1524
Fragments arranged by size:
1676 1524 787 597 518 393 268 140
14
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 2765 2378
Size: 387 5530
EcoO109I rG'GnC_Cy
Cuts at: 2279 2314 2489 2279
Size: 35 175 5707
Fragments arranged by size:
5707 175 35
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 2432 2723 2811 3149 5064 1678
Size: 754 291 88 338 1915 2531
Fragments arranged by size:
2531 1915 754 338 291 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4558 4590 4632 4664 1373
Size: 32 135 32 2986 32 42 32 2626
Fragments arranged by size:
2986 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3261 3507 669
Size: 988 281 1323 246 3079
Fragments arranged by size:
3079 1323 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KasI G'GCGC_C
Cuts at: 2428 2719 2807 2428
Size: 291 88 5538
Fragments arranged by size:
5538 291 88
KpnI G_GTAC'C
Cuts at: 2974 3242 2974
Size: 268 5649
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2247 2595 5031 5215 1305
Size: 942 348 2436 184 2007
Fragments arranged by size:
2436 2007 942 348 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2207 10
Size: 54 1425 343 375 3720
Fragments arranged by size:
3720 1425 375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2453 2561 2666 2928 3875 4234
Size: 648 861 426 108 105 262 947 359
Cuts at: 4234 4393 518
Size: 159 2042
Fragments arranged by size:
2042 947 861 648 426 359 262 159
108 105
MspA1I CmG'CkG
Cuts at: 1606 2710 2813 3166 3333 4010 5158 5403 1606
Size: 1104 103 353 167 677 1148 245 2120
Fragments arranged by size:
2120 1148 1104 677 353 245 167 103
NarI GG'CG_CC
Cuts at: 2429 2720 2808 2429
Size: 291 88 5538
Fragments arranged by size:
5538 291 88
NcoI C'CATG_G
Cuts at: 513 1065 2715 2820 2848 513
Size: 552 1650 105 28 3582
Fragments arranged by size:
3582 1650 552 105 28
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 2236 2566 3275 4820 1132
Size: 97 1007 330 709 1545 2229
Fragments arranged by size:
2229 1545 1007 709 330 97
PasI CC'CwG_GG
Cuts at: 1348 3067 1348
Size: 1719 4198
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5917
PfoI T'CCnGG_A
Cuts at: 2127 2127
Size: 5917
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PmlI CAC'GTG
Cuts at: 2410 2410
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2489 2489
Size: 5917
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PspOMI G'GGCC_C
Cuts at: 2278 2468 2278
Size: 190 5727
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2183 2215 2183
Size: 32 5885
PstI C_TGCA'G
Cuts at: 830 2382 3269 830
Size: 1552 887 3478
Fragments arranged by size:
3478 1552 887
PvuI CG_AT'CG
Cuts at: 2001 2214 2001
Size: 213 5704
SacII CC_GC'GG
Cuts at: 2814 2814
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SanDI GG'GwC_CC
Cuts at: 2489 2489
Size: 5917
SapI GCTCTTCn'nnn_
Cuts at: 2752 2752
Size: 5917
SbfI CC_TGCA'GG
Cuts at: 2382 3269 2382
Size: 887 5030
ScaI AGT'ACT
Cuts at: 2777 2834 4165 5877 2777
Size: 57 1331 1712 2817
Fragments arranged by size:
2817 1712 1331 57
SexAI A'CCwGG_T
Cuts at: 2193 2193
Size: 5917
SfcI C'TryA_G
Cuts at: 826 1045 2378 2476 2614 3265 4400 5081 5272
Size: 219 1333 98 138 651 1135 681 191
Cuts at: 5272 826
Size: 1471
Fragments arranged by size:
1471 1333 1135 681 651 219 191 138
98
SfoI GGC'GCC
Cuts at: 2430 2721 2809 2430
Size: 291 88 5538
Fragments arranged by size:
5538 291 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 3040 3244 1872
Size: 1168 204 4545
Fragments arranged by size:
4545 1168 204
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4021 4922 5184 820
Size: 197 926 2078 901 262 1553
Fragments arranged by size:
2078 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3214 3708 493
Size: 2721 494 2702
Fragments arranged by size:
2721 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5917
StyI C'CwwG_G
Cuts at: 513 1065 2715 2820 2848 3106 3356 513
Size: 552 1650 105 28 258 250 3074
Fragments arranged by size:
3074 1650 552 258 250 105 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3944 3961 4114 4299 4744 325
Size: 1255 2364 17 153 185 445 1498
Fragments arranged by size:
2364 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2775 2790 2832
Size: 274 80 33 51 1443 798 15 42
Cuts at: 2832 2949 4163 5875 96
Size: 117 1214 1712 138
Fragments arranged by size:
1712 1443 1214 798 274 138 117 80
51 42 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5877 5909 5877
Size: 32 5885
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5917
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2668 2668
Size: 5917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 3038 3242 1870
Size: 1168 204 4545
Fragments arranged by size:
4545 1168 204
XmnI GAAnn'nnTTC
Cuts at: 4046 4046
Size: 5917
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI
PvuII RsrII SacI SfiI SrfI SwaI