Restriction map for
pFN21ASDA0690
(Circular) (Six-base) MAPSORT of: pFN21ASDA0690.seq Check: 1347 from: 1 to: 8320
pFN21ASDA0690 8320 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6130 278
Size: 53 83 186 936 4594 2468
Fragments arranged by size:
4594 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5608 5663 1937
Size: 3671 55 4594
Fragments arranged by size:
4594 3671 55
Acc65I G'GTAC_C
Cuts at: 2300 5641 2300
Size: 3341 4979
AclI AA'CG_TT
Cuts at: 1563 6447 6820 1563
Size: 4884 373 3063
Fragments arranged by size:
4884 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2131 2326 2675 3071 3530 3783 4193 6383
Size: 499 195 349 396 459 253 410 2190
Cuts at: 6383 7767 8035 1632
Size: 1384 268 1917
Fragments arranged by size:
2190 1917 1384 499 459 410 396 349
268 253 195
AfeI AGC'GCT
Cuts at: 2072 2357 2072
Size: 285 8035
AflII C'TTAA_G
Cuts at: 820 1017 2894 820
Size: 197 1877 6246
Fragments arranged by size:
6246 1877 197
AflIII A'CryG_T
Cuts at: 1203 4663 7219 8196 1203
Size: 3460 2556 977 1327
Fragments arranged by size:
3460 2556 1327 977
AgeI A'CCGG_T
Cuts at: 7151 7151
Size: 8320
AhdI GACnn_n'nnGTC
Cuts at: 7049 7049
Size: 8320
AleI CACnn'nnGTG
Cuts at: 1166 2420 3577 1166
Size: 1254 1157 5909
Fragments arranged by size:
5909 1254 1157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8320
AlwNI CAG_nnn'CTG
Cuts at: 3113 3202 3268 3715 3752 4552 4634 6063 7635
Size: 89 66 447 37 800 82 1429 1572
Cuts at: 7635 3113
Size: 3798
Fragments arranged by size:
3798 1572 1429 800 447 89 82 66
37
ApaI G_GGCC'C
Cuts at: 4136 4144 4136
Size: 8 8312
ApaLI G'TGCA_C
Cuts at: 4315 6377 7533 4315
Size: 2062 1156 5102
Fragments arranged by size:
5102 2062 1156
ApoI r'AATT_y
Cuts at: 1493 2525 3167 5629 5855 6007 8116 1493
Size: 1032 642 2462 226 152 2109 1697
Fragments arranged by size:
2462 2109 1697 1032 642 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6874 160
Size: 872 5842 1606
Fragments arranged by size:
5842 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8320
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2296 4136 4796 4842 5645 1870
Size: 73 89 264 1840 660 46 803 4545
Fragments arranged by size:
4545 1840 803 660 264 89 73 46
BamHI G'GATC_C
Cuts at: 5086 5467 5650 6017 5086
Size: 381 183 367 7389
Fragments arranged by size:
7389 381 367 183
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 4854 5231 5641 7096
Size: 325 217 627 513 2554 377 410 1455
Cuts at: 7096 618
Size: 1842
Fragments arranged by size:
2554 1842 1455 627 513 410 377 325
217
BanII G_rGCy'C
Cuts at: 1391 2820 3188 4136 4144 4864 4917 5073 5639
Size: 1429 368 948 8 720 53 156 566
Cuts at: 5639 1391
Size: 4072
Fragments arranged by size:
4072 1429 948 720 566 368 156 53
8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3202 4184 928
Size: 971 1303 982 5064
Fragments arranged by size:
5064 1303 982 971
BbvCI CC'TCA_GC
Cuts at: 4836 5325 4836
Size: 489 7831
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6511 6545 1903
Size: 34 4574 34 3678
Fragments arranged by size:
4574 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6212 7422 6212
Size: 1210 7110
BclI T'GATC_A
Cuts at: 1548 5184 5277 1548
Size: 3636 93 4591
Fragments arranged by size:
4591 3636 93
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2762 4455 5399
Size: 107 122 71 631 605 1090 1693 944
Cuts at: 5399 6931 136
Size: 1532 1525
Fragments arranged by size:
1693 1532 1525 1090 944 631 605 122
107 71
BglII A'GATC_T
Cuts at: 2391 3561 8315 2391
Size: 1170 4754 2396
Fragments arranged by size:
4754 2396 1170
BlpI GC'TnA_GC
Cuts at: 5737 5737
Size: 8320
Bme1580I G_kGCm'C
Cuts at: 1790 4136 4144 4319 6381 7537 1790
Size: 2346 8 175 2062 1156 2573
Fragments arranged by size:
2573 2346 2062 1156 175 8
BmgBI CAC'GTC
Cuts at: 1318 4594 4645 1318
Size: 3276 51 4993
Fragments arranged by size:
4993 3276 51
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2853 4619 6051
Size: 416 53 83 399 24 1436 1766 1432
Cuts at: 6051 7009 442
Size: 958 1753
Fragments arranged by size:
1766 1753 1436 1432 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2888 1056
Size: 1832 6488
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2311 2389 2311
Size: 78 8242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2628 3202 3245 3560 3825 4127 4184 4574
Size: 1389 574 43 315 265 302 57 390
Cuts at: 4574 5360 5385 6980 1239
Size: 786 25 1595 2579
Fragments arranged by size:
2579 1595 1389 786 574 390 315 302
265 57 43 25
Bpu10I CC'TnA_GC
Cuts at: 2798 3534 4836 4865 5325 2798
Size: 736 1302 29 460 5793
Fragments arranged by size:
5793 1302 736 460 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 2749 3023 5291 6445 7310 7608 2498
Size: 251 274 2268 1154 865 298 3210
Fragments arranged by size:
3210 2268 1154 865 298 274 251
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3438 6983 882
Size: 617 1939 3545 2219
Fragments arranged by size:
3545 2219 1939 617
BsaAI yAC'GTr
Cuts at: 493 1204 5617 6111 493
Size: 711 4413 494 2702
Fragments arranged by size:
4413 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8314 8314
Size: 8320
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2252 6127 6509 7144
Size: 53 83 186 936 719 3875 382 635
Cuts at: 7144 275
Size: 1451
Fragments arranged by size:
3875 1451 936 719 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2478 4827 6753 7151 7425 7572 7983 2478
Size: 2349 1926 398 274 147 411 2815
Fragments arranged by size:
2815 2349 1926 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 3071 3101 4100
Size: 30 165 30 1041 30 597 30 999
Cuts at: 4100 4130 4547 4577 1178
Size: 30 417 30 4921
Fragments arranged by size:
4921 1041 999 597 417 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 3088 3164 3413 4124 4198 4814 4898
Size: 1516 386 76 249 711 74 616 84
Cuts at: 4898 4910 4913 4916 4919 4922 4925 4958 4985
Size: 12 3 3 3 3 3 33 27
Cuts at: 4985 4997 5000 5047 5210 5342 5345 5348 1186
Size: 12 3 47 163 132 3 3 4158
Fragments arranged by size:
4158 1516 711 616 386 249 163 132
84 76 74 47 33 27 12 12
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2490 3661 7523 8019 2490
Size: 1171 3862 496 2791
Fragments arranged by size:
3862 2791 1171 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2311 2621 3595 4103 4172 4590 4688
Size: 573 575 310 974 508 69 418 98
Cuts at: 4688 1163
Size: 4795
Fragments arranged by size:
4795 974 575 573 508 418 310 98
69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2156 5465 5770 6531 7559 1540
Size: 275 186 155 3309 305 761 1028 2301
Fragments arranged by size:
3309 2301 1028 761 305 275 186 155
BsiHKAI G_wGCw'C
Cuts at: 2804 2820 3188 3945 4205 4319 6381 6466 7537
Size: 16 368 757 260 114 2062 85 1071
Cuts at: 7537 2804
Size: 3587
Fragments arranged by size:
3587 2062 1071 757 368 260 114 85
16
BsmI GAATG_Cn'
Cuts at: 3062 5376 5831 5924 3062
Size: 2314 455 93 5458
Fragments arranged by size:
5458 2314 455 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2243 2441 2243
Size: 198 8122
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2804 2820 3188 3945 4136 4144 4205
Size: 399 1014 16 368 757 191 8 61
Cuts at: 4205 4319 4864 4917 5073 5639 6381 6466 7537
Size: 114 545 53 156 566 742 85 1071
Cuts at: 7537 1391
Size: 2174
Fragments arranged by size:
2174 1071 1014 757 742 566 545 399
368 191 156 114 85 61 53 16
8
BspEI T'CCGG_A
Cuts at: 2478 4827 7983 2478
Size: 2349 3156 2815
Fragments arranged by size:
3156 2815 2349
BspHI T'CATG_A
Cuts at: 2721 6209 7939 2721
Size: 3488 1730 3102
Fragments arranged by size:
3488 3102 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2670 2996 3285 5675 844
Size: 1826 326 289 2390 3489
Fragments arranged by size:
3489 2390 1826 326 289
BsrBI CCG'CTC
Cuts at: 1394 1823 6207 1394
Size: 429 4384 3507
Fragments arranged by size:
4384 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6815 6989 65
Size: 1183 5567 174 1396
Fragments arranged by size:
5567 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2151 5178 5460 6964 7151 1700
Size: 110 341 3027 282 1504 187 2869
Fragments arranged by size:
3027 2869 1504 341 282 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8320
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8320
BssSI C'ACGA_G
Cuts at: 4364 6380 7392 4364
Size: 2016 1012 5292
Fragments arranged by size:
5292 2016 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3373 794
Size: 2579 5741
BstBI TT'CG_AA
Cuts at: 2007 7124 2007
Size: 5117 3203
BstEII G'GTnAC_C
Cuts at: 1184 2983 3337 3697 4462 1184
Size: 1799 354 360 765 5042
Fragments arranged by size:
5042 1799 765 360 354
BstXI CCAn_nnnn'nTGG
Cuts at: 3423 3529 4540 3423
Size: 106 1011 7203
Fragments arranged by size:
7203 1011 106
BstYI r'GATC_y
Cuts at: 1972 2391 3045 3561 5086 5467 5650 6017 6402
Size: 419 654 516 1525 381 183 367 385
Cuts at: 6402 6419 7871 7957 7969 8315 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1525 1452 654 516 419 385 381
367 346 183 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2714 3106 3354 3692 4128 4388 2714
Size: 392 248 338 436 260 6646
Fragments arranged by size:
6646 436 392 338 260 248
BtgI C'CryG_G
Cuts at: 513 1065 2022 2256 3416 3522 3822 4075 4467
Size: 552 957 234 1160 106 300 253 392
Cuts at: 4467 4878 513
Size: 411 3955
Fragments arranged by size:
3955 1160 957 552 411 392 300 253
234 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5366 487
Size: 683 56 4140 3441
Fragments arranged by size:
4140 3441 683 56
BtsI GCAGTG_nn'
Cuts at: 2527 2571 2707 2942 3150 3330 4181 4287 4358
Size: 44 136 235 208 180 851 106 71
Cuts at: 4358 4427 4635 5836 6630 6650 2527
Size: 69 208 1201 794 20 4197
Fragments arranged by size:
4197 1201 851 794 235 208 208 180
136 106 71 69 44 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8285
DraI TTT'AAA
Cuts at: 4516 5624 4516
Size: 1108 7212
DraIII CAC_nnn'GTG
Cuts at: 3244 3705 4081 4252 3244
Size: 461 376 171 7312
Fragments arranged by size:
7312 461 376 171
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2177 3695 7327 809
Size: 507 861 1518 3632 1802
Fragments arranged by size:
3632 1802 1518 861 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2153 3630 3669
Size: 54 1425 50 275 18 323 1477 39
Cuts at: 3669 4656 5112 5176 5391 5462 5767 6656 8285
Size: 987 456 64 215 71 305 889 1629
Cuts at: 8285 8
Size: 43
Fragments arranged by size:
1629 1477 1425 987 889 456 323 305
275 215 71 64 54 50 43 39
18
EagI C'GGCC_G
Cuts at: 1537 1812 2153 5462 5767 1537
Size: 275 341 3309 305 4090
Fragments arranged by size:
4090 3309 341 305 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 2687 2783 3506 3806 4151 4404 4887
Size: 906 428 96 723 300 345 253 483
Cuts at: 4887 5217 5244 6250 1353
Size: 330 27 1006 3423
Fragments arranged by size:
3423 1006 906 723 483 428 345 330
300 253 96 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2285 6907 7281 7427 387
Size: 1064 138 696 4622 374 146 1280
Fragments arranged by size:
4622 1280 1064 696 374 146 138
EcoICRI GAG'CTC
Cuts at: 2818 3186 2818
Size: 368 7952
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2131 2326 2628 2675 3071 3202 3245
Size: 393 499 195 302 47 396 131 43
Cuts at: 3245 3530 3560 3783 3825 4127 4184 4193 4574
Size: 285 30 223 42 302 57 9 381
Cuts at: 4574 5360 5385 6383 6980 7767 8035 1239
Size: 786 25 998 597 787 268 1524
Fragments arranged by size:
1524 998 787 786 597 499 396 393
381 302 302 285 268 223 195 131
57 47 43 42 30 25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3533 4868 5552 3533
Size: 1335 684 6301
Fragments arranged by size:
6301 1335 684
EcoO109I rG'GnC_Cy
Cuts at: 2217 2542 2659 2941 3110 3154 3350 3712 4132
Size: 325 117 282 169 44 196 362 420
Cuts at: 4132 4133 4140 4141 4168 4224 4414 2217
Size: 1 7 1 27 56 190 6123
Fragments arranged by size:
6123 420 362 325 282 196 190 169
117 56 44 27 7 1 1
EcoRI G'AATT_C
Cuts at: 2525 5629 6007 2525
Size: 3104 378 4838
Fragments arranged by size:
4838 3104 378
FseI GG_CCGG'CC
Cuts at: 5464 5464
Size: 8320
FspI TGC'GCA
Cuts at: 3719 6826 3719
Size: 3107 5213
HaeII r_GCGC'y
Cuts at: 1678 2074 2359 5172 7467 1678
Size: 396 285 2813 2295 2531
Fragments arranged by size:
2813 2531 2295 396 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 3071 3103 3963
Size: 32 135 32 623 32 844 32 860
Cuts at: 3963 3995 4803 4835 5335 5367 6961 6993 7035
Size: 32 808 32 500 32 1594 32 42
Cuts at: 7035 7067 1373
Size: 32 2626
Fragments arranged by size:
2626 1594 860 844 808 623 500 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4822 5609 5664 5910 669
Size: 988 281 2884 787 55 246 3079
Fragments arranged by size:
3079 2884 988 787 281 246 55
HindIII A'AGCT_T
Cuts at: 748 4694 4730 748
Size: 3946 36 4338
Fragments arranged by size:
4338 3946 36
HpaI GTT'AAC
Cuts at: 5910 5910
Size: 8320
KpnI G_GTAC'C
Cuts at: 2304 5645 2304
Size: 3341 4979
MfeI C'AATT_G
Cuts at: 5919 5919
Size: 8320
MluI A'CGCG_T
Cuts at: 8196 8196
Size: 8320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2346 2394 7434 7618 1305
Size: 1041 48 5040 184 2007
Fragments arranged by size:
5040 2007 1041 184 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3632 3671 4658 5114 5393
Size: 54 1425 343 1800 39 987 456 279
Cuts at: 5393 10
Size: 2937
Fragments arranged by size:
2937 1800 1425 987 456 343 279 54
39
MslI CAynn'nnrTG
Cuts at: 518 1166 2420 3577 4622 4643 6278 6637 6796
Size: 648 1254 1157 1045 21 1635 359 159
Cuts at: 6796 518
Size: 2042
Fragments arranged by size:
2042 1635 1254 1157 1045 648 359 159
21
MspA1I CmG'CkG
Cuts at: 1606 3199 4268 4476 4549 4672 5014 5736 6413
Size: 1593 1069 208 73 123 342 722 677
Cuts at: 6413 7561 7806 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1593 1148 1069 722 677 342 245
208 123 73
NaeI GCC'GGC
Cuts at: 2153 5180 5462 2153
Size: 3027 282 5011
Fragments arranged by size:
5011 3027 282
NcoI C'CATG_G
Cuts at: 513 1065 2022 2256 3522 4467 513
Size: 552 957 234 1266 945 4366
Fragments arranged by size:
4366 1266 957 945 552 234
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8320
NgoMIV G'CCGG_C
Cuts at: 2151 5178 5460 2151
Size: 3027 282 5011
Fragments arranged by size:
5011 3027 282
NheI G'CTAG_C
Cuts at: 1052 2884 1052
Size: 1832 6488
NotI GC'GGCC_GC
Cuts at: 5767 5767
Size: 8320
NspI r_CATG'y
Cuts at: 1132 1229 2644 4667 4711 5678 7223 1132
Size: 97 1415 2023 44 967 1545 2229
Fragments arranged by size:
2229 2023 1545 1415 967 97 44
PasI CC'CwG_GG
Cuts at: 1348 2434 4921 1348
Size: 1086 2487 4747
Fragments arranged by size:
4747 2487 1086
PciI A'CATG_T
Cuts at: 4663 7219 4663
Size: 2556 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2740 3325 1108
Size: 1632 585 6103
Fragments arranged by size:
6103 1632 585
PfoI T'CCnGG_A
Cuts at: 2589 2676 3918 3981 5082 2589
Size: 87 1242 63 1101 5827
Fragments arranged by size:
5827 1242 1101 87 63
PmeI GTTT'AAAC
Cuts at: 5624 5624
Size: 8320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3435 3467 6389 6421 7902 7934 3435
Size: 32 2922 32 1481 32 3821
Fragments arranged by size:
3821 2922 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2217 2659 2941 3350 4168 2217
Size: 442 282 409 818 6369
Fragments arranged by size:
6369 818 442 409 282
PshAI GACnn'nnGTC
Cuts at: 1613 4469 1613
Size: 2856 5464
PsiI TTA'TAA
Cuts at: 5890 5890
Size: 8320
PspOMI G'GGCC_C
Cuts at: 4132 4140 4132
Size: 8 8312
PstI C_TGCA'G
Cuts at: 830 2211 2702 3369 4422 4481 5532 5672 830
Size: 1381 491 667 1053 59 1051 140 3478
Fragments arranged by size:
3478 1381 1053 1051 667 491 140 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8320
PvuII CAG'CTG
Cuts at: 3199 4268 4476 4549 4672 3199
Size: 1069 208 73 123 6847
Fragments arranged by size:
6847 1069 208 123 73
SacI G_AGCT'C
Cuts at: 2820 3188 2820
Size: 368 7952
SalI G'TCGA_C
Cuts at: 1936 5607 5662 1936
Size: 3671 55 4594
Fragments arranged by size:
4594 3671 55
SapI GCTCTTCn'nnn_
Cuts at: 2687 3506 2687
Size: 819 7501
SbfI CC_TGCA'GG
Cuts at: 5672 5672
Size: 8320
ScaI AGT'ACT
Cuts at: 2462 6568 8280 2462
Size: 4106 1712 2502
Fragments arranged by size:
4106 2502 1712
SexAI A'CCwGG_T
Cuts at: 2922 3987 2922
Size: 1065 7255
SfcI C'TryA_G
Cuts at: 826 1045 2207 2698 3365 3621 4418 4477 5528
Size: 219 1162 491 667 256 797 59 1051
Cuts at: 5528 5668 6803 7484 7675 826
Size: 140 1135 681 191 1471
Fragments arranged by size:
1471 1162 1135 1051 797 681 667 491
256 219 191 140 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8320
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8320
SmaI CCC'GGG
Cuts at: 1872 4138 4844 5647 1872
Size: 2266 706 803 4545
Fragments arranged by size:
4545 2266 803 706
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 2728 2894 3038 5306 6424
Size: 197 926 570 215 166 144 2268 1118
Cuts at: 6424 7325 7587 820
Size: 901 262 1553
Fragments arranged by size:
2268 1553 1118 926 901 570 262 215
197 166 144
SnaBI TAC'GTA
Cuts at: 493 5617 6111 493
Size: 5124 494 2702
Fragments arranged by size:
5124 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8320
SphI G_CATG'C
Cuts at: 1132 5678 1132
Size: 4546 3774
SrfI GCCC'GGGC
Cuts at: 4844 4844
Size: 8320
SspI AAT'ATT
Cuts at: 5 52 6244 5
Size: 47 6192 2081
Fragments arranged by size:
6192 2081 47
StuI AGG'CCT
Cuts at: 1517 2685 2765 2791 3619 4094 5547 1517
Size: 1168 80 26 828 475 1453 4290
Fragments arranged by size:
4290 1453 1168 828 475 80 26
StyI C'CwwG_G
Cuts at: 513 1065 2022 2256 2616 3522 3732 3999 4320
Size: 552 957 234 360 906 210 267 321
Cuts at: 4320 4467 5091 5759 513
Size: 147 624 668 3074
Fragments arranged by size:
3074 957 906 668 624 552 360 321
267 234 210 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6347 6364 6517 6702 7147 325
Size: 1255 4767 17 153 185 445 1498
Fragments arranged by size:
4767 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2350 2460 4535
Size: 274 80 33 51 1443 373 110 2075
Cuts at: 4535 6566 8278 96
Size: 2031 1712 138
Fragments arranged by size:
2075 2031 1712 1443 373 274 138 110
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2967 2999 3342 3374 4100 4132 4547 4579 8280
Size: 32 343 32 726 32 415 32 3701
Cuts at: 8280 8312 2967
Size: 32 2975
Fragments arranged by size:
3701 2975 726 415 343 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3989 4595 1316
Size: 2673 606 5041
Fragments arranged by size:
5041 2673 606
XbaI T'CTAG_A
Cuts at: 5656 5656
Size: 8320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2789 2789
Size: 8320
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8320
XmaI C'CCGG_G
Cuts at: 1870 4136 4842 5645 1870
Size: 2266 706 803 4545
Fragments arranged by size:
4545 2266 803 706
XmnI GAAnn'nnTTC
Cuts at: 4742 6449 4742
Size: 1707 6613
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6128 276
Size: 53 83 186 936 4594 2468
Fragments arranged by size:
4594 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoRV FalI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SwaI