Restriction map for
pFN21ASDA0685
(Circular) (Six-base) MAPSORT of: pFN21ASDA0685.seq Check: 2162 from: 1 to: 7510
pFN21ASDA0685 7510 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4001 4001
Size: 7510
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2568 5320 278
Size: 53 83 186 936 1032 2752 2468
Fragments arranged by size:
2752 2468 1032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4853 1937
Size: 2916 4594
Acc65I G'GTAC_C
Cuts at: 4831 4831
Size: 7510
AclI AA'CG_TT
Cuts at: 1563 5637 6010 1563
Size: 4074 373 3063
Fragments arranged by size:
4074 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2798 2978 3909 4303 5573 6957 7225 1632
Size: 1166 180 931 394 1270 1384 268 1917
Fragments arranged by size:
1917 1384 1270 1166 931 394 268 180
AfeI AGC'GCT
Cuts at: 2505 2745 2505
Size: 240 7270
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7313
AflIII A'CryG_T
Cuts at: 1203 2784 4347 6409 7386 1203
Size: 1581 1563 2062 977 1327
Fragments arranged by size:
2062 1581 1563 1327 977
AgeI A'CCGG_T
Cuts at: 6341 6341
Size: 7510
AhdI GACnn_n'nnGTC
Cuts at: 6239 6239
Size: 7510
AleI CACnn'nnGTG
Cuts at: 1166 3347 1166
Size: 2181 5329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2667 2723 1184
Size: 1483 56 5971
Fragments arranged by size:
5971 1483 56
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 2036
Size: 32 7478
AlwNI CAG_nnn'CTG
Cuts at: 2152 2776 2902 2977 3493 5253 6825 2152
Size: 624 126 75 516 1760 1572 2837
Fragments arranged by size:
2837 1760 1572 624 516 126 75
ApaI G_GGCC'C
Cuts at: 3506 4245 4713 3506
Size: 739 468 6303
Fragments arranged by size:
6303 739 468
ApaLI G'TGCA_C
Cuts at: 5567 6723 5567
Size: 1156 6354
ApoI r'AATT_y
Cuts at: 1493 4819 5045 5197 7306 1493
Size: 3326 226 152 2109 1697
Fragments arranged by size:
3326 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6064 160
Size: 872 5032 1606
Fragments arranged by size:
5032 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7510
AvaI C'yCGr_G
Cuts at: 1870 1943 2623 3885 4028 4610 4835 1870
Size: 73 680 1262 143 582 225 4545
Fragments arranged by size:
4545 1262 680 582 225 143 73
AvrII C'CTAG_G
Cuts at: 4705 4705
Size: 7510
BamHI G'GATC_C
Cuts at: 2212 3257 3387 4840 5207 2212
Size: 1045 130 1453 367 4515
Fragments arranged by size:
4515 1453 1045 367 130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2495 3059 4013 4073 4169
Size: 325 217 627 708 564 954 60 96
Cuts at: 4169 4831 6286 618
Size: 662 1455 1842
Fragments arranged by size:
1842 1455 954 708 662 627 564 325
217 96 60
BanII G_rGCy'C
Cuts at: 1391 2556 3036 3117 3506 3543 3916 4245 4406
Size: 1165 480 81 389 37 373 329 161
Cuts at: 4406 4713 4829 1391
Size: 307 116 4072
Fragments arranged by size:
4072 1165 480 389 373 329 307 161
116 81 37
BbeI G_GCGC'C
Cuts at: 3063 4077 3063
Size: 1014 6496
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6539
BbvCI CC'TCA_GC
Cuts at: 2500 4174 4284 2500
Size: 1674 110 5726
Fragments arranged by size:
5726 1674 110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5701 5735 1903
Size: 34 3764 34 3678
Fragments arranged by size:
3764 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5402 6612 5402
Size: 1210 6300
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7510
BfrBI ATG'CAT
Cuts at: 3204 3204
Size: 7510
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2551 4022 4745
Size: 107 122 71 631 605 879 1471 723
Cuts at: 4745 6121 136
Size: 1376 1525
Fragments arranged by size:
1525 1471 1376 879 723 631 605 122
107 71
BglII A'GATC_T
Cuts at: 7505 7505
Size: 7510
BlpI GC'TnA_GC
Cuts at: 2330 4927 2330
Size: 2597 4913
Bme1580I G_kGCm'C
Cuts at: 1790 3506 4018 4245 4608 4713 5571 6727 1790
Size: 1716 512 227 363 105 858 1156 2573
Fragments arranged by size:
2573 1716 1156 858 512 363 227 105
BmgBI CAC'GTC
Cuts at: 1318 2052 3443 4144 1318
Size: 734 1391 701 4684
Fragments arranged by size:
4684 1391 734 701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5241 6199 442
Size: 416 53 83 399 24 3824 958 1753
Fragments arranged by size:
3824 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7510
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3952 4078 3952
Size: 126 7384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2741 2768 3047 3326 3413 3515 4164 6170
Size: 1502 27 279 279 87 102 649 2006
Cuts at: 6170 1239
Size: 2579
Fragments arranged by size:
2579 2006 1502 649 279 279 102 87
27
Bpu10I CC'TnA_GC
Cuts at: 2500 4174 4284 2500
Size: 1674 110 5726
Fragments arranged by size:
5726 1674 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5635 6500 6798 5635
Size: 865 298 6347
Fragments arranged by size:
6347 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2685 2790 6173 882
Size: 617 1186 105 3383 2219
Fragments arranged by size:
3383 2219 1186 617 105
BsaAI yAC'GTr
Cuts at: 493 1204 4807 5301 493
Size: 711 3603 494 2702
Fragments arranged by size:
3603 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7504 7504
Size: 7510
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2565 3060 4074 5317
Size: 53 83 186 936 1032 495 1014 1243
Cuts at: 5317 5699 6334 275
Size: 382 635 1451
Fragments arranged by size:
1451 1243 1032 1014 936 635 495 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2799 3297 4258 5943 6341 6615 6762 7173 2799
Size: 498 961 1685 398 274 147 411 3136
Fragments arranged by size:
3136 1685 961 498 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4162 4192 4376 4406 1178
Size: 30 165 30 2759 30 184 30 4282
Fragments arranged by size:
4282 2759 184 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 2246 2726 3044 3251 3596 3707 4199
Size: 1015 45 480 318 207 345 111 492
Cuts at: 4199 4384 4523 1186
Size: 185 139 4173
Fragments arranged by size:
4173 1015 492 480 345 318 207 185
139 111 45
BseYI C'CCAG_C
Cuts at: 2683 3098 3315 3564 3782 4194 4591 4669 4735
Size: 415 217 249 218 412 397 78 66
Cuts at: 4735 6713 7209 2683
Size: 1978 496 2984
Fragments arranged by size:
2984 1978 496 415 412 397 249 218
217 78 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2188 2555 3068 3401 3506 3530 4270
Size: 573 452 367 513 333 105 24 740
Cuts at: 4270 4668 1163
Size: 398 4005
Fragments arranged by size:
4005 740 573 513 452 398 367 333
105 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2305 3324 4471 4518 4960 5721
Size: 275 186 304 1019 1147 47 442 761
Cuts at: 5721 6749 1540
Size: 1028 2301
Fragments arranged by size:
2301 1147 1028 1019 761 442 304 275
186 47
BsiHKAI G_wGCw'C
Cuts at: 3117 5571 5656 6727 3117
Size: 2454 85 1071 3900
Fragments arranged by size:
3900 2454 1071 85
BsmI GAATG_Cn'
Cuts at: 3049 4277 5021 5114 3049
Size: 1228 744 93 5445
Fragments arranged by size:
5445 1228 744 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3433 3578 3590 3433
Size: 145 12 7353
Fragments arranged by size:
7353 145 12
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2556 3036 3117 3506 3543 3916 4018
Size: 399 766 480 81 389 37 373 102
Cuts at: 4018 4245 4406 4608 4713 4829 5571 5656 6727
Size: 227 161 202 105 116 742 85 1071
Cuts at: 6727 1391
Size: 2174
Fragments arranged by size:
2174 1071 766 742 480 399 389 373
227 202 161 116 105 102 85 81
37
BspEI T'CCGG_A
Cuts at: 7173 7173
Size: 7510
BspHI T'CATG_A
Cuts at: 5399 7129 5399
Size: 1730 5780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2523 4001 4865 844
Size: 1679 1478 864 3489
Fragments arranged by size:
3489 1679 1478 864
BsrBI CCG'CTC
Cuts at: 1394 1823 2734 3499 5397 1394
Size: 429 911 765 1898 3507
Fragments arranged by size:
3507 1898 911 765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2415 6005 6179 65
Size: 1183 1167 3590 174 1396
Fragments arranged by size:
3590 1396 1183 1167 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3120 3319 4021 4542 6154 6341 1700
Size: 110 1310 199 702 521 1612 187 2869
Fragments arranged by size:
2869 1612 1310 702 521 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7510
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7510
BssSI C'ACGA_G
Cuts at: 5570 6582 5570
Size: 1012 6498
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4439 794
Size: 3645 3865
BstBI TT'CG_AA
Cuts at: 2007 6314 2007
Size: 4307 3203
BstEII G'GTnAC_C
Cuts at: 1184 3349 1184
Size: 2165 5345
BstXI CCAn_nnnn'nTGG
Cuts at: 3481 3667 4341 3481
Size: 186 674 6650
Fragments arranged by size:
6650 674 186
BstYI r'GATC_y
Cuts at: 1972 2212 3257 3387 3642 4840 5207 5592 5609
Size: 240 1045 130 255 1198 367 385 17
Cuts at: 5609 7061 7147 7159 7505 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1198 1045 385 367 346 255
240 130 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3556 4443 4596 4692 4740 513
Size: 552 2491 887 153 96 48 3283
Fragments arranged by size:
3283 2491 887 552 153 96 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6771
Fragments arranged by size:
6771 683 56
BtsI GCAGTG_nn'
Cuts at: 2183 3077 3817 4095 5026 5820 5840 2183
Size: 894 740 278 931 794 20 3853
Fragments arranged by size:
3853 931 894 794 740 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7510
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7475
DraI TTT'AAA
Cuts at: 4814 4814
Size: 7510
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6517 809
Size: 507 5201 1802
Fragments arranged by size:
5201 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3321 3559 3894
Size: 54 1425 50 275 18 1491 238 335
Cuts at: 3894 4468 4497 4623 4689 4746 4957 5846 7475
Size: 574 29 126 66 57 211 889 1629
Cuts at: 7475 8
Size: 43
Fragments arranged by size:
1629 1491 1425 889 574 335 275 238
211 126 66 57 54 50 43 29
18
EagI C'GGCC_G
Cuts at: 1537 1812 3321 4468 4957 1537
Size: 275 1509 1147 489 4090
Fragments arranged by size:
4090 1509 1147 489 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2580 4684 5440 1353
Size: 1227 2104 756 3423
Fragments arranged by size:
3423 2104 1227 756
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2205 6097 6471 6617 387
Size: 1064 138 616 3892 374 146 1280
Fragments arranged by size:
3892 1280 1064 616 374 146 138
EcoICRI GAG'CTC
Cuts at: 3115 3115
Size: 7510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2741 2768 2798 2978 3047 3326 3413
Size: 393 1109 27 30 180 69 279 87
Cuts at: 3413 3515 3909 4164 4303 5573 6170 6957 7225
Size: 102 394 255 139 1270 597 787 268
Cuts at: 7225 1239
Size: 1524
Fragments arranged by size:
1524 1270 1109 787 597 394 393 279
268 255 180 139 102 87 69 30
27
EcoNI CCTnn'n_nnAGG
Cuts at: 4008 4008
Size: 7510
EcoO109I rG'GnC_Cy
Cuts at: 3502 3503 4241 4304 4366 4463 4477 4709 3502
Size: 1 738 63 62 97 14 232 6303
Fragments arranged by size:
6303 738 232 97 63 62 14 1
EcoRI G'AATT_C
Cuts at: 4819 5197 4819
Size: 378 7132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2260 3997 2056
Size: 204 1737 5569
Fragments arranged by size:
5569 1737 204
FspI TGC'GCA
Cuts at: 6016 6016
Size: 7510
HaeII r_GCGC'y
Cuts at: 1678 2507 2747 3063 4077 6657 1678
Size: 829 240 316 1014 2580 2531
Fragments arranged by size:
2580 2531 1014 829 316 240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2630 2662 4376 4408 4447
Size: 32 135 32 1058 32 1714 32 39
Cuts at: 4447 4479 6151 6183 6225 6257 1373
Size: 32 1672 32 42 32 2626
Fragments arranged by size:
2626 1714 1672 1058 135 42 39 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2062 4854 5100 669
Size: 988 281 124 2792 246 3079
Fragments arranged by size:
3079 2792 988 281 246 124
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7510
HpaI GTT'AAC
Cuts at: 5100 5100
Size: 7510
KasI G'GCGC_C
Cuts at: 3059 4073 3059
Size: 1014 6496
KpnI G_GTAC'C
Cuts at: 4835 4835
Size: 7510
MfeI C'AATT_G
Cuts at: 5109 5109
Size: 7510
MluI A'CGCG_T
Cuts at: 7386 7386
Size: 7510
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4240 6624 6808 1305
Size: 2935 2384 184 2007
Fragments arranged by size:
2935 2384 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3896 4499 4625 4748 10
Size: 54 1425 343 2064 603 126 123 2772
Fragments arranged by size:
2772 2064 1425 603 343 126 123 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3020 3347 3996 5468 5827 5986 518
Size: 648 1854 327 649 1472 359 159 2042
Fragments arranged by size:
2042 1854 1472 649 648 359 327 159
MspA1I CmG'CkG
Cuts at: 1606 2535 2707 3255 3428 3564 4166 4486 4926
Size: 929 172 548 173 136 602 320 440
Cuts at: 4926 5603 6751 6996 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 929 677 602 548 440 320
245 173 172 136
NaeI GCC'GGC
Cuts at: 3122 3321 4544 3122
Size: 199 1223 6088
Fragments arranged by size:
6088 1223 199
NarI GG'CG_CC
Cuts at: 3060 4074 3060
Size: 1014 6496
NcoI C'CATG_G
Cuts at: 513 1065 4443 4740 513
Size: 552 3378 297 3283
Fragments arranged by size:
3378 3283 552 297
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7510
NgoMIV G'CCGG_C
Cuts at: 3120 3319 4542 3120
Size: 199 1223 6088
Fragments arranged by size:
6088 1223 199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7510
NotI GC'GGCC_GC
Cuts at: 4957 4957
Size: 7510
NsiI A_TGCA'T
Cuts at: 3206 3206
Size: 7510
NspI r_CATG'y
Cuts at: 1132 1229 2087 2788 4868 6413 1132
Size: 97 858 701 2080 1545 2229
Fragments arranged by size:
2229 2080 1545 858 701 97
PasI CC'CwG_GG
Cuts at: 1348 3037 4753 1348
Size: 1689 1716 4105
Fragments arranged by size:
4105 1716 1689
PciI A'CATG_T
Cuts at: 2784 6409 2784
Size: 3625 3885
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 3956 4158 1108
Size: 920 1928 202 4460
Fragments arranged by size:
4460 1928 920 202
PfoI T'CCnGG_A
Cuts at: 3390 3390
Size: 7510
PmeI GTTT'AAAC
Cuts at: 4814 4814
Size: 7510
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5579 5611 7092 7124 5579
Size: 32 1481 32 5965
Fragments arranged by size:
5965 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4463 4463
Size: 7510
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7510
PsiI TTA'TAA
Cuts at: 5080 5080
Size: 7510
PspOMI G'GGCC_C
Cuts at: 3502 4241 4709 3502
Size: 739 468 6303
Fragments arranged by size:
6303 739 468
PstI C_TGCA'G
Cuts at: 830 2097 3812 4862 830
Size: 1267 1715 1050 3478
Fragments arranged by size:
3478 1715 1267 1050
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7510
PvuII CAG'CTG
Cuts at: 2535 2707 4166 2535
Size: 172 1459 5879
Fragments arranged by size:
5879 1459 172
SacI G_AGCT'C
Cuts at: 3117 3117
Size: 7510
SalI G'TCGA_C
Cuts at: 1936 4852 1936
Size: 2916 4594
SanDI GG'GwC_CC
Cuts at: 4463 4463
Size: 7510
SbfI CC_TGCA'GG
Cuts at: 4862 4862
Size: 7510
ScaI AGT'ACT
Cuts at: 5758 7470 5758
Size: 1712 5798
SfcI C'TryA_G
Cuts at: 826 1045 2093 3808 4858 5993 6674 6865 826
Size: 219 1048 1715 1050 1135 681 191 1471
Fragments arranged by size:
1715 1471 1135 1050 1048 681 219 191
SfoI GGC'GCC
Cuts at: 3061 4075 3061
Size: 1014 6496
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7510
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7510
SmaI CCC'GGG
Cuts at: 1872 3887 4030 4837 1872
Size: 2015 143 807 4545
Fragments arranged by size:
4545 2015 807 143
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5614 6515 6777 820
Size: 197 926 3671 901 262 1553
Fragments arranged by size:
3671 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4807 5301 493
Size: 4314 494 2702
Fragments arranged by size:
4314 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7510
SphI G_CATG'C
Cuts at: 1132 4868 1132
Size: 3736 3774
SspI AAT'ATT
Cuts at: 5 52 5434 5
Size: 47 5382 2081
Fragments arranged by size:
5382 2081 47
StuI AGG'CCT
Cuts at: 1517 3398 3803 1517
Size: 1881 405 5224
Fragments arranged by size:
5224 1881 405
StyI C'CwwG_G
Cuts at: 513 1065 4300 4443 4705 4740 4949 513
Size: 552 3235 143 262 35 209 3074
Fragments arranged by size:
3235 3074 552 262 209 143 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3112 5537 5554 5707 5892 6337 325
Size: 1255 1532 2425 17 153 185 445 1498
Fragments arranged by size:
2425 1532 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3162 5756 7468
Size: 274 80 33 51 1443 1185 2594 1712
Cuts at: 7468 96
Size: 138
Fragments arranged by size:
2594 1712 1443 1185 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2150 2182 2197 2229 4162 4194 4336 4368 7470
Size: 32 15 32 1933 32 142 32 3102
Cuts at: 7470 7502 2150
Size: 32 2158
Fragments arranged by size:
3102 2158 1933 142 32 32 32 32
32 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7510
XbaI T'CTAG_A
Cuts at: 4846 4846
Size: 7510
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3022 4415 3022
Size: 1393 6117
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7510
XmaI C'CCGG_G
Cuts at: 1870 3885 4028 4835 1870
Size: 2015 143 807 4545
Fragments arranged by size:
4545 2015 807 143
XmnI GAAnn'nnTTC
Cuts at: 2963 4316 5639 2963
Size: 1353 1323 4834
Fragments arranged by size:
4834 1353 1323
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2566 5318 276
Size: 53 83 186 936 1032 2752 2468
Fragments arranged by size:
2752 2468 1032 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI NruI PacI PmlI PspXI PsrI RsrII SacII
SapI SexAI SfiI SrfI SwaI