Restriction map for
pFN21ASDA0680
(Circular) (Six-base) MAPSORT of: pFN21ASDA0680.seq Check: 7142 from: 1 to: 6631
pFN21ASDA0680 6631 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3832 3832
Size: 6631
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4441 278
Size: 53 83 186 936 2905 2468
Fragments arranged by size:
2905 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3898 3974 1937
Size: 1961 76 4594
Fragments arranged by size:
4594 1961 76
Acc65I G'GTAC_C
Cuts at: 3952 3952
Size: 6631
AclI AA'CG_TT
Cuts at: 1563 4758 5131 1563
Size: 3195 373 3063
Fragments arranged by size:
3195 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2397 3171 3276 3549 3624 4694 6078 6346
Size: 765 774 105 273 75 1070 1384 268
Cuts at: 6346 1632
Size: 1917
Fragments arranged by size:
1917 1384 1070 774 765 273 268 105
75
AflII C'TTAA_G
Cuts at: 820 1017 2264 3193 820
Size: 197 1247 929 4258
Fragments arranged by size:
4258 1247 929 197
AflIII A'CryG_T
Cuts at: 1203 5530 6507 1203
Size: 4327 977 1327
Fragments arranged by size:
4327 1327 977
AgeI A'CCGG_T
Cuts at: 5462 5462
Size: 6631
AhdI GACnn_n'nnGTC
Cuts at: 5360 5360
Size: 6631
AleI CACnn'nnGTG
Cuts at: 1166 2580 1166
Size: 1414 5217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6631
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2931 2963 2931
Size: 32 6599
AlwNI CAG_nnn'CTG
Cuts at: 2480 3188 3265 4374 5946 2480
Size: 708 77 1109 1572 3165
Fragments arranged by size:
3165 1572 1109 708 77
ApaLI G'TGCA_C
Cuts at: 4688 5844 4688
Size: 1156 5475
ApoI r'AATT_y
Cuts at: 1493 2183 2311 2978 3860 3940 4166 4318 6427
Size: 690 128 667 882 80 226 152 2109
Cuts at: 6427 1493
Size: 1697
Fragments arranged by size:
2109 1697 882 690 667 226 152 128
80
AseI AT'TA_AT
Cuts at: 160 1032 5185 160
Size: 872 4153 1606
Fragments arranged by size:
4153 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
AvaI C'yCGr_G
Cuts at: 1870 1943 2352 2673 3956 1870
Size: 73 409 321 1283 4545
Fragments arranged by size:
4545 1283 409 321 73
BamHI G'GATC_C
Cuts at: 3738 3961 4328 3738
Size: 223 367 6041
Fragments arranged by size:
6041 367 223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3952 5407 618
Size: 325 217 627 2165 1455 1842
Fragments arranged by size:
2165 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3950 1391
Size: 2559 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2177 3254 3399 928
Size: 971 278 1077 145 4160
Fragments arranged by size:
4160 1077 971 278 145
BbvCI CC'TCA_GC
Cuts at: 2958 3258 2958
Size: 300 6331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2712 2746 4822 4856 1903
Size: 34 775 34 2076 34 3678
Fragments arranged by size:
3678 2076 775 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3445 4523 5733 3445
Size: 1078 1210 4343
Fragments arranged by size:
4343 1210 1078
BclI T'GATC_A
Cuts at: 1548 2278 1548
Size: 730 5901
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5242 136
Size: 107 122 71 631 605 3570 1525
Fragments arranged by size:
3570 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6626 6626
Size: 6631
BlpI GC'TnA_GC
Cuts at: 3584 3698 4048 3584
Size: 114 350 6167
Fragments arranged by size:
6167 350 114
Bme1580I G_kGCm'C
Cuts at: 1790 2325 4692 5848 1790
Size: 535 2367 1156 2573
Fragments arranged by size:
2573 2367 1156 535
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6631
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4362 5320 442
Size: 416 53 83 399 24 2945 958 1753
Fragments arranged by size:
2945 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6631
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3471 3471
Size: 6631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2438 2973 2997 3063 5291 1239
Size: 1199 535 24 66 2228 2579
Fragments arranged by size:
2579 2228 1199 535 66 24
Bpu10I CC'TnA_GC
Cuts at: 2505 2958 3258 2505
Size: 453 300 5878
Fragments arranged by size:
5878 453 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3068 4756 5621 5919 3068
Size: 1688 865 298 3780
Fragments arranged by size:
3780 1688 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2906 3700 5294 882
Size: 617 1407 794 1594 2219
Fragments arranged by size:
2219 1594 1407 794 617
BsaAI yAC'GTr
Cuts at: 493 1204 3928 4422 493
Size: 711 2724 494 2702
Fragments arranged by size:
2724 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2333 6625 2333
Size: 4292 2339
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4438 4820 5455 275
Size: 53 83 186 936 2905 382 635 1451
Fragments arranged by size:
2905 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5064 5462 5736 5883 6294 5064
Size: 398 274 147 411 5401
Fragments arranged by size:
5401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2602 2632 3760 3790 1178
Size: 30 165 30 1199 30 1128 30 4019
Fragments arranged by size:
4019 1199 1128 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2490 2680 2975 3015 3251 1186
Size: 1069 235 190 295 40 236 4566
Fragments arranged by size:
4566 1069 295 236 235 190 40
BseYI C'CCAG_C
Cuts at: 2564 3241 5834 6330 2564
Size: 677 2593 496 2865
Fragments arranged by size:
2865 2593 677 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6058
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4081 4842 5870 1540
Size: 275 186 2080 761 1028 2301
Fragments arranged by size:
2301 2080 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4692 4777 5848 4692
Size: 85 1071 5475
Fragments arranged by size:
5475 1071 85
BsmI GAATG_Cn'
Cuts at: 4142 4235 4142
Size: 93 6538
BsmBI CGTCTCn'nnnn_
Cuts at: 3121 3121
Size: 6631
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2325 3950 4692 4777 5848 1391
Size: 399 535 1625 742 85 1071 2174
Fragments arranged by size:
2174 1625 1071 742 535 399 85
BspEI T'CCGG_A
Cuts at: 6294 6294
Size: 6631
BspHI T'CATG_A
Cuts at: 3886 4520 6250 3886
Size: 634 1730 4267
Fragments arranged by size:
4267 1730 634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2075 3117 3832 3986 844
Size: 1231 1042 715 154 3489
Fragments arranged by size:
3489 1231 1042 715 154
BsrBI CCG'CTC
Cuts at: 1394 1823 4518 1394
Size: 429 2695 3507
Fragments arranged by size:
3507 2695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5126 5300 65
Size: 1183 3878 174 1396
Fragments arranged by size:
3878 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2470 5275 5462 1700
Size: 110 660 2805 187 2869
Fragments arranged by size:
2869 2805 660 187 110
BsrGI T'GTAC_A
Cuts at: 96 3372 96
Size: 3276 3355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6631
BssSI C'ACGA_G
Cuts at: 4691 5703 4691
Size: 1012 5619
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2480 794
Size: 1686 4945
BstBI TT'CG_AA
Cuts at: 2007 5435 2007
Size: 3428 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6631
BstYI r'GATC_y
Cuts at: 1972 2513 3738 3961 4328 4713 4730 6182 6268
Size: 541 1225 223 367 385 17 1452 86
Cuts at: 6268 6280 6626 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1225 541 385 367 346 223
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2988 513
Size: 552 957 966 4156
Fragments arranged by size:
4156 966 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5892
Fragments arranged by size:
5892 683 56
BtsI GCAGTG_nn'
Cuts at: 2559 3418 4147 4941 4961 2559
Size: 859 729 794 20 4229
Fragments arranged by size:
4229 859 794 729 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6596
DraI TTT'AAA
Cuts at: 3865 3935 3865
Size: 70 6561
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3113 5638 809
Size: 507 1797 2525 1802
Fragments arranged by size:
2525 1802 1797 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4078 4967 6596
Size: 54 1425 50 275 18 2248 889 1629
Cuts at: 6596 8
Size: 43
Fragments arranged by size:
2248 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4078 1537
Size: 275 2266 4090
Fragments arranged by size:
4090 2266 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3147 3528 3642 3885 4561 1353
Size: 1794 381 114 243 676 3423
Fragments arranged by size:
3423 1794 676 381 243 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5218 5592 5738 387
Size: 1064 138 3629 374 146 1280
Fragments arranged by size:
3629 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2397 2438 2973 2997 3063 3171 3276
Size: 393 765 41 535 24 66 108 105
Cuts at: 3276 3549 3624 4694 5291 6078 6346 1239
Size: 273 75 1070 597 787 268 1524
Fragments arranged by size:
1524 1070 787 765 597 535 393 273
268 108 105 75 66 41 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2550 3811 2550
Size: 1261 5370
EcoO109I rG'GnC_Cy
Cuts at: 2632 2632
Size: 6631
EcoRI G'AATT_C
Cuts at: 3940 4318 3940
Size: 378 6253
EcoRV GAT'ATC
Cuts at: 2220 2220
Size: 6631
FspI TGC'GCA
Cuts at: 5137 5137
Size: 6631
HaeII r_GCGC'y
Cuts at: 1678 5778 1678
Size: 4100 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3241 3273 3338 3370 5272
Size: 32 135 32 1669 32 65 32 1902
Cuts at: 5272 5304 5346 5378 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1902 1669 135 65 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2038 2302 3202 3819 3899 3975
Size: 988 281 100 264 900 617 80 76
Cuts at: 3975 4221 669
Size: 246 3079
Fragments arranged by size:
3079 988 900 617 281 264 246 100
80 76
HindIII A'AGCT_T
Cuts at: 748 2052 2766 748
Size: 1304 714 4613
Fragments arranged by size:
4613 1304 714
HpaI GTT'AAC
Cuts at: 2302 3202 3819 4221 2302
Size: 900 617 402 4712
Fragments arranged by size:
4712 900 617 402
KpnI G_GTAC'C
Cuts at: 3956 3956
Size: 6631
MfeI C'AATT_G
Cuts at: 4230 4230
Size: 6631
MluI A'CGCG_T
Cuts at: 6507 6507
Size: 6631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2913 3079 3574 5745 5929 1305
Size: 1608 166 495 2171 184 2007
Fragments arranged by size:
2171 2007 1608 495 184 166
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4809
Fragments arranged by size:
4809 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2580 3875 4589 4948 5107 518
Size: 648 1414 1295 714 359 159 2042
Fragments arranged by size:
2042 1414 1295 714 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4047 4724 5872 6117 1606
Size: 2441 677 1148 245 2120
Fragments arranged by size:
2441 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2988 513
Size: 552 957 966 4156
Fragments arranged by size:
4156 966 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6631
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6631
NotI GC'GGCC_GC
Cuts at: 4078 4078
Size: 6631
NspI r_CATG'y
Cuts at: 1132 1229 3989 5534 1132
Size: 97 2760 1545 2229
Fragments arranged by size:
2760 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3807 1348
Size: 2459 4172
PciI A'CATG_T
Cuts at: 5530 5530
Size: 6631
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6631
PmeI GTTT'AAAC
Cuts at: 3935 3935
Size: 6631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4700 4732 6213 6245 4700
Size: 32 1481 32 5086
Fragments arranged by size:
5086 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3132 1613
Size: 1519 5112
PsiI TTA'TAA
Cuts at: 4201 4201
Size: 6631
PstI C_TGCA'G
Cuts at: 830 2911 3829 3983 830
Size: 2081 918 154 3478
Fragments arranged by size:
3478 2081 918 154
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6631
SalI G'TCGA_C
Cuts at: 1936 3897 3973 1936
Size: 1961 76 4594
Fragments arranged by size:
4594 1961 76
SapI GCTCTTCn'nnn_
Cuts at: 3147 3147
Size: 6631
SbfI CC_TGCA'GG
Cuts at: 3983 3983
Size: 6631
ScaI AGT'ACT
Cuts at: 4879 6591 4879
Size: 1712 4919
SfcI C'TryA_G
Cuts at: 826 1045 2058 2907 3825 3979 5114 5795 5986
Size: 219 1013 849 918 154 1135 681 191
Cuts at: 5986 826
Size: 1471
Fragments arranged by size:
1471 1135 1013 918 849 681 219 191
154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6631
SmaI CCC'GGG
Cuts at: 1872 3958 1872
Size: 2086 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 3047 3193 4735 5636 5898
Size: 197 926 321 783 146 1542 901 262
Cuts at: 5898 820
Size: 1553
Fragments arranged by size:
1553 1542 926 901 783 321 262 197
146
SnaBI TAC'GTA
Cuts at: 493 3928 4422 493
Size: 3435 494 2702
Fragments arranged by size:
3435 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6631
SphI G_CATG'C
Cuts at: 1132 3989 1132
Size: 2857 3774
SspI AAT'ATT
Cuts at: 5 52 4555 5
Size: 47 4503 2081
Fragments arranged by size:
4503 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6631
StyI C'CwwG_G
Cuts at: 513 1065 2022 2150 2613 2814 2988 4070 513
Size: 552 957 128 463 201 174 1082 3074
Fragments arranged by size:
3074 1082 957 552 463 201 174 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2662 4658 4675 4828 5013 5458 325
Size: 1255 1082 1996 17 153 185 445 1498
Fragments arranged by size:
1996 1498 1255 1082 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3372 4877 6589
Size: 274 80 33 51 1443 1395 1505 1712
Cuts at: 6589 96
Size: 138
Fragments arranged by size:
1712 1505 1443 1395 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3760 3792 6591 6623 3760
Size: 32 2799 32 3768
Fragments arranged by size:
3768 2799 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6631
XbaI T'CTAG_A
Cuts at: 3967 3967
Size: 6631
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3238 3238
Size: 6631
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6631
XmaI C'CCGG_G
Cuts at: 1870 3956 1870
Size: 2086 4545
XmnI GAAnn'nnTTC
Cuts at: 4760 4760
Size: 6631
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4439 276
Size: 53 83 186 936 2905 2468
Fragments arranged by size:
2905 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI