Restriction map for
pFN21ASDA0679
(Circular) (Six-base) MAPSORT of: pFN21ASDA0679.seq Check: 8376 from: 1 to: 7477
pFN21ASDA0679 7477 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5287 278
Size: 53 83 186 936 3751 2468
Fragments arranged by size:
3751 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4436 4820 1937
Size: 2499 384 4594
Fragments arranged by size:
4594 2499 384
Acc65I G'GTAC_C
Cuts at: 4798 4798
Size: 7477
AclI AA'CG_TT
Cuts at: 1563 5604 5977 1563
Size: 4041 373 3063
Fragments arranged by size:
4041 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4008 4646 5540 6924 7192 1632
Size: 2376 638 894 1384 268 1917
Fragments arranged by size:
2376 1917 1384 894 638 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7280
AflIII A'CryG_T
Cuts at: 1203 3939 6376 7353 1203
Size: 2736 2437 977 1327
Fragments arranged by size:
2736 2437 1327 977
AgeI A'CCGG_T
Cuts at: 6308 6308
Size: 7477
AhdI GACnn_n'nnGTC
Cuts at: 6206 6206
Size: 7477
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7477
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3720 1184
Size: 2536 4941
AlwNI CAG_nnn'CTG
Cuts at: 5220 6792 5220
Size: 1572 5905
ApaI G_GGCC'C
Cuts at: 2906 2906
Size: 7477
ApaLI G'TGCA_C
Cuts at: 5534 6690 5534
Size: 1156 6321
ApoI r'AATT_y
Cuts at: 1493 2796 2972 3764 4404 4680 4688 4786 5012
Size: 1303 176 792 640 276 8 98 226
Cuts at: 5012 5164 7273 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1303 792 640 276 226 176
152 98 8
AseI AT'TA_AT
Cuts at: 160 1032 6031 160
Size: 872 4999 1606
Fragments arranged by size:
4999 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7477
AvaI C'yCGr_G
Cuts at: 1870 1943 4641 4802 1870
Size: 73 2698 161 4545
Fragments arranged by size:
4545 2698 161 73
AvrII C'CTAG_G
Cuts at: 2638 2638
Size: 7477
BamHI G'GATC_C
Cuts at: 4413 4807 5174 4413
Size: 394 367 6716
Fragments arranged by size:
6716 394 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 4798 6253 618
Size: 325 217 627 341 2670 1455 1842
Fragments arranged by size:
2670 1842 1455 627 341 325 217
BanII G_rGCy'C
Cuts at: 1391 2073 2194 2845 2906 3330 4796 1391
Size: 682 121 651 61 424 1466 4072
Fragments arranged by size:
4072 1466 682 651 424 121 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2145 2239 4611 928
Size: 971 246 94 2372 3794
Fragments arranged by size:
3794 2372 971 246 94
BbvCI CC'TCA_GC
Cuts at: 4307 4307
Size: 7477
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5668 5702 1903
Size: 34 3731 34 3678
Fragments arranged by size:
3731 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2485 5369 6579 2485
Size: 2884 1210 3383
Fragments arranged by size:
3383 2884 1210
BclI T'GATC_A
Cuts at: 1548 2881 1548
Size: 1333 6144
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6088 136
Size: 107 122 71 631 605 4416 1525
Fragments arranged by size:
4416 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7472 7472
Size: 7477
BlpI GC'TnA_GC
Cuts at: 2072 2370 4894 2072
Size: 298 2524 4655
Fragments arranged by size:
4655 2524 298
Bme1580I G_kGCm'C
Cuts at: 1790 2906 4370 5538 6694 1790
Size: 1116 1464 1168 1156 2573
Fragments arranged by size:
2573 1464 1168 1156 1116
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7477
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5208 6166 442
Size: 416 53 83 399 24 3791 958 1753
Fragments arranged by size:
3791 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3170 1056
Size: 2114 5363
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 7477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2261 2829 3707 6137 1239
Size: 890 132 568 878 2430 2579
Fragments arranged by size:
2579 2430 890 878 568 132
Bpu10I CC'TnA_GC
Cuts at: 4307 4307
Size: 7477
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3373 5602 6467 6765 3373
Size: 2229 865 298 4085
Fragments arranged by size:
4085 2229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4038 4657 6140 882
Size: 617 2539 619 1483 2219
Fragments arranged by size:
2539 2219 1483 619 617
BsaAI yAC'GTr
Cuts at: 493 1204 4774 5268 493
Size: 711 3570 494 2702
Fragments arranged by size:
3570 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2880 7471 2880
Size: 4591 2886
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5284 5666 6301 275
Size: 53 83 186 936 3751 382 635 1451
Fragments arranged by size:
3751 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5910 6308 6582 6729 7140 5910
Size: 398 274 147 411 6247
Fragments arranged by size:
6247 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2154 2184 3929 3959 1178
Size: 30 165 30 751 30 1745 30 4696
Fragments arranged by size:
4696 1745 751 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 1186
Size: 883 6594
BseYI C'CCAG_C
Cuts at: 2156 6680 7176 2156
Size: 4524 496 2457
Fragments arranged by size:
4524 2457 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 2382 2539 2586 1163
Size: 573 312 334 157 47 6054
Fragments arranged by size:
6054 573 334 312 157 47
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4927 5688 6716 1540
Size: 275 186 2926 761 1028 2301
Fragments arranged by size:
2926 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 2845 3751 5538 5623 6694 2073
Size: 772 906 1787 85 1071 2856
Fragments arranged by size:
2856 1787 1071 906 772 85
BsmI GAATG_Cn'
Cuts at: 4988 5081 4988
Size: 93 7384
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2194 2845 2906 3330 3751 4370
Size: 399 283 121 651 61 424 421 619
Cuts at: 4370 4796 5538 5623 6694 1391
Size: 426 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 619 426 424 421
399 283 121 85 61
BspEI T'CCGG_A
Cuts at: 7140 7140
Size: 7477
BspHI T'CATG_A
Cuts at: 5366 7096 5366
Size: 1730 5747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4832 844
Size: 3988 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 2165 5364 1394
Size: 429 236 106 3199 3507
Fragments arranged by size:
3507 3199 429 236 106
BsrDI GCAATG_nn'
Cuts at: 65 1248 3591 5972 6146 65
Size: 1183 2343 2381 174 1396
Fragments arranged by size:
2381 2343 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2116 6121 6308 1700
Size: 110 306 4005 187 2869
Fragments arranged by size:
4005 2869 306 187 110
BsrGI T'GTAC_A
Cuts at: 96 3663 3994 4206 96
Size: 3567 331 212 3367
Fragments arranged by size:
3567 3367 331 212
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7477
BssSI C'ACGA_G
Cuts at: 2400 3849 5537 6549 2400
Size: 1449 1688 1012 3328
Fragments arranged by size:
3328 1688 1449 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3637 794
Size: 2843 4634
BstBI TT'CG_AA
Cuts at: 2007 6281 2007
Size: 4274 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7477
BstXI CCAn_nnnn'nTGG
Cuts at: 2853 3421 2853
Size: 568 6909
BstYI r'GATC_y
Cuts at: 1972 2918 4413 4807 5174 5559 5576 7028 7114
Size: 946 1495 394 367 385 17 1452 86
Cuts at: 7114 7126 7472 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1495 1452 946 394 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 4437 4437
Size: 7477
Bsu36I CC'TnA_GG
Cuts at: 4188 4188
Size: 7477
BtgI C'CryG_G
Cuts at: 513 1065 2022 2936 3414 513
Size: 552 957 914 478 4576
Fragments arranged by size:
4576 957 914 552 478
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2088 487
Size: 683 56 862 5876
Fragments arranged by size:
5876 862 683 56
BtsI GCAGTG_nn'
Cuts at: 3118 3666 4993 5787 5807 3118
Size: 548 1327 794 20 4788
Fragments arranged by size:
4788 1327 794 548 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7477
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4728 4763 529
Size: 35 4164 35 3243
Fragments arranged by size:
4164 3243 35 35
DraI TTT'AAA
Cuts at: 3291 4781 3291
Size: 1490 5987
DraIII CAC_nnn'GTG
Cuts at: 3236 3236
Size: 7477
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6484 809
Size: 507 5168 1802
Fragments arranged by size:
5168 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 4590 4924
Size: 54 1425 50 275 18 342 2418 334
Cuts at: 4924 5813 7442 8
Size: 889 1629 43
Fragments arranged by size:
2418 1629 1425 889 342 334 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4924 1537
Size: 275 3112 4090
Fragments arranged by size:
4090 3112 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2666 2811 2882 3861 5407 1353
Size: 1313 145 71 979 1546 3423
Fragments arranged by size:
3423 1546 1313 979 145 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6064 6438 6584 387
Size: 1064 138 4475 374 146 1280
Fragments arranged by size:
4475 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2071 2843 2071
Size: 772 6705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2261 2829 3707 4008 4646 5540
Size: 393 497 132 568 878 301 638 894
Cuts at: 5540 6137 6924 7192 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 894 878 787 638 597 568 497
393 301 268 132
EcoO109I rG'GnC_Cy
Cuts at: 2774 2774
Size: 7477
EcoRI G'AATT_C
Cuts at: 4680 4786 5164 4680
Size: 106 378 6993
Fragments arranged by size:
6993 378 106
EcoRV GAT'ATC
Cuts at: 2449 3961 2449
Size: 1512 5965
FspI TGC'GCA
Cuts at: 5983 5983
Size: 7477
HaeII r_GCGC'y
Cuts at: 1678 6624 1678
Size: 4946 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2433 2465 3929 3961 4184
Size: 32 135 32 861 32 1464 32 223
Cuts at: 4184 4216 6118 6150 6192 6224 1373
Size: 32 1902 32 42 32 2626
Fragments arranged by size:
2626 1902 1464 861 223 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4821 5067 669
Size: 988 281 2883 246 3079
Fragments arranged by size:
3079 2883 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2741 748
Size: 1993 5484
HpaI GTT'AAC
Cuts at: 5067 5067
Size: 7477
KpnI G_GTAC'C
Cuts at: 4802 4802
Size: 7477
MfeI C'AATT_G
Cuts at: 3805 3910 4104 5076 3805
Size: 105 194 972 6206
Fragments arranged by size:
6206 972 194 105
MluI A'CGCG_T
Cuts at: 7353 7353
Size: 7477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2031 2103 4742 6591 6775 1305
Size: 726 72 2639 1849 184 2007
Fragments arranged by size:
2639 2007 1849 726 184 72
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4592 10
Size: 54 1425 343 2760 2895
Fragments arranged by size:
2895 2760 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2871 3419 3425 3456 5435 5794 5953
Size: 648 1705 548 6 31 1979 359 159
Cuts at: 5953 518
Size: 2042
Fragments arranged by size:
2042 1979 1705 648 548 359 159 31
6
MspA1I CmG'CkG
Cuts at: 1606 4893 5570 6718 6963 1606
Size: 3287 677 1148 245 2120
Fragments arranged by size:
3287 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2118 2118
Size: 7477
NcoI C'CATG_G
Cuts at: 513 1065 2022 3414 513
Size: 552 957 1392 4576
Fragments arranged by size:
4576 1392 957 552
NdeI CA'TA_TG
Cuts at: 387 4349 387
Size: 3962 3515
NgoMIV G'CCGG_C
Cuts at: 2116 2116
Size: 7477
NheI G'CTAG_C
Cuts at: 1052 3166 1052
Size: 2114 5363
NotI GC'GGCC_GC
Cuts at: 4924 4924
Size: 7477
NspI r_CATG'y
Cuts at: 1132 1229 3943 4835 6380 1132
Size: 97 2714 892 1545 2229
Fragments arranged by size:
2714 2229 1545 892 97
PasI CC'CwG_GG
Cuts at: 1348 4056 1348
Size: 2708 4769
PciI A'CATG_T
Cuts at: 3939 6376 3939
Size: 2437 5040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4109 1108
Size: 3001 4476
PfoI T'CCnGG_A
Cuts at: 4409 4409
Size: 7477
PmeI GTTT'AAAC
Cuts at: 4781 4781
Size: 7477
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2402 2434 5546 5578 7059 7091 2402
Size: 32 3112 32 1481 32 2788
Fragments arranged by size:
3112 2788 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2774 2774
Size: 7477
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7477
PsiI TTA'TAA
Cuts at: 5047 5047
Size: 7477
PspOMI G'GGCC_C
Cuts at: 2902 2902
Size: 7477
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4494 4526 4494
Size: 32 7445
PstI C_TGCA'G
Cuts at: 830 2185 4829 830
Size: 1355 2644 3478
Fragments arranged by size:
3478 2644 1355
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7477
SacI G_AGCT'C
Cuts at: 2073 2845 2073
Size: 772 6705
SalI G'TCGA_C
Cuts at: 1936 4819 1936
Size: 2883 4594
SbfI CC_TGCA'GG
Cuts at: 4829 4829
Size: 7477
ScaI AGT'ACT
Cuts at: 3076 4512 5725 7437 3076
Size: 1436 1213 1712 3116
Fragments arranged by size:
3116 1712 1436 1213
SexAI A'CCwGG_T
Cuts at: 2499 3011 2499
Size: 512 6965
SfcI C'TryA_G
Cuts at: 826 1045 2181 3142 4371 4825 5960 6641 6832
Size: 219 1136 961 1229 454 1135 681 191
Cuts at: 6832 826
Size: 1471
Fragments arranged by size:
1471 1229 1136 1135 961 681 454 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7477
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7477
SmaI CCC'GGG
Cuts at: 1872 4643 4804 1872
Size: 2771 161 4545
Fragments arranged by size:
4545 2771 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3352 5581 6482 6744 820
Size: 197 926 1409 2229 901 262 1553
Fragments arranged by size:
2229 1553 1409 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4774 5268 493
Size: 4281 494 2702
Fragments arranged by size:
4281 2702 494
SpeI A'CTAG_T
Cuts at: 152 3880 152
Size: 3728 3749
SphI G_CATG'C
Cuts at: 1132 4835 1132
Size: 3703 3774
SspI AAT'ATT
Cuts at: 5 52 5401 5
Size: 47 5349 2081
Fragments arranged by size:
5349 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7477
StyI C'CwwG_G
Cuts at: 513 1065 2022 2237 2466 2638 3378 3414 4362
Size: 552 957 215 229 172 740 36 948
Cuts at: 4362 4916 513
Size: 554 3074
Fragments arranged by size:
3074 957 948 740 554 552 229 215
172 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2505 2723 5504 5521 5674 5859 6304
Size: 1255 925 218 2781 17 153 185 445
Cuts at: 6304 325
Size: 1498
Fragments arranged by size:
2781 1498 1255 925 445 218 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3074 3136 3663
Size: 274 80 33 51 1443 1097 62 527
Cuts at: 3663 3942 3994 4206 4510 5723 7435 96
Size: 279 52 212 304 1213 1712 138
Fragments arranged by size:
1712 1443 1213 1097 527 304 279 274
212 138 80 62 52 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2154 2186 2719 2745 2751 2777 7437 7469 2154
Size: 32 533 26 6 26 4660 32 2162
Fragments arranged by size:
4660 2162 533 32 32 26 26 6
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7477
XbaI T'CTAG_A
Cuts at: 4813 4813
Size: 7477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3421 4600 3421
Size: 1179 6298
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7477
XmaI C'CCGG_G
Cuts at: 1870 4641 4802 1870
Size: 2771 161 4545
Fragments arranged by size:
4545 2771 161
XmnI GAAnn'nnTTC
Cuts at: 2742 4734 5606 2742
Size: 1992 872 4613
Fragments arranged by size:
4613 1992 872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5285 276
Size: 53 83 186 936 3751 2468
Fragments arranged by size:
3751 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI
BsmBI EcoNI FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PvuII RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI