Restriction map for
pFN21ASDA0663
(Circular) (Six-base) MAPSORT of: pFN21ASDA0663.seq Check: 5499 from: 1 to: 7159
pFN21ASDA0663 7159 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4969 278
Size: 53 83 186 936 3433 2468
Fragments arranged by size:
3433 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4502 1937
Size: 2565 4594
Acc65I G'GTAC_C
Cuts at: 4480 4480
Size: 7159
AclI AA'CG_TT
Cuts at: 1563 5286 5659 1563
Size: 3723 373 3063
Fragments arranged by size:
3723 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2601 2715 3183 3575 3633 4194 4209 5222
Size: 969 114 468 392 58 561 15 1013
Cuts at: 5222 6606 6874 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1013 969 561 468 392 268
114 58 15
AflII C'TTAA_G
Cuts at: 820 1017 3014 820
Size: 197 1997 4965
Fragments arranged by size:
4965 1997 197
AflIII A'CryG_T
Cuts at: 1203 2066 6058 7035 1203
Size: 863 3992 977 1327
Fragments arranged by size:
3992 1327 977 863
AgeI A'CCGG_T
Cuts at: 5990 5990
Size: 7159
AhdI GACnn_n'nnGTC
Cuts at: 5888 5888
Size: 7159
AleI CACnn'nnGTG
Cuts at: 1166 3821 1166
Size: 2655 4504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7159
AlwNI CAG_nnn'CTG
Cuts at: 2777 4166 4902 6474 2777
Size: 1389 736 1572 3462
Fragments arranged by size:
3462 1572 1389 736
ApaLI G'TGCA_C
Cuts at: 3428 5216 6372 3428
Size: 1788 1156 4215
Fragments arranged by size:
4215 1788 1156
ApoI r'AATT_y
Cuts at: 1493 2211 2653 2885 3111 3681 4468 4694 4846
Size: 718 442 232 226 570 787 226 152
Cuts at: 4846 6955 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 787 718 570 442 232 226
226 152
AseI AT'TA_AT
Cuts at: 160 1032 2493 5713 160
Size: 872 1461 3220 1606
Fragments arranged by size:
3220 1606 1461 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
AvaI C'yCGr_G
Cuts at: 1870 1943 4484 1870
Size: 73 2541 4545
Fragments arranged by size:
4545 2541 73
BamHI G'GATC_C
Cuts at: 4396 4489 4856 4396
Size: 93 367 6699
Fragments arranged by size:
6699 367 93
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3840 4480 5935 618
Size: 325 217 627 2053 640 1455 1842
Fragments arranged by size:
2053 1842 1455 640 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2766 3488 4478 1391
Size: 1375 722 990 4072
Fragments arranged by size:
4072 1375 990 722
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2046 3104 928
Size: 971 147 1058 4983
Fragments arranged by size:
4983 1058 971 147
BbvCI CC'TCA_GC
Cuts at: 2421 2421
Size: 7159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2151 2185 5350 5384 1903
Size: 34 214 34 3165 34 3678
Fragments arranged by size:
3678 3165 214 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3298 5051 6261 3298
Size: 1753 1210 4196
Fragments arranged by size:
4196 1753 1210
BclI T'GATC_A
Cuts at: 1548 2524 1548
Size: 976 6183
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4118 5770 136
Size: 107 122 71 631 605 2446 1652 1525
Fragments arranged by size:
2446 1652 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7154 7154
Size: 7159
BlpI GC'TnA_GC
Cuts at: 4576 4576
Size: 7159
Bme1580I G_kGCm'C
Cuts at: 1790 3432 5220 6376 1790
Size: 1642 1788 1156 2573
Fragments arranged by size:
2573 1788 1642 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7159
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2882 4210 4890
Size: 416 53 83 399 24 1465 1328 680
Cuts at: 4890 5848 442
Size: 958 1753
Fragments arranged by size:
1753 1465 1328 958 680 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2370 1056
Size: 1314 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2946 3479 3513 4069 5819 1239
Size: 1707 533 34 556 1750 2579
Fragments arranged by size:
2579 1750 1707 556 533 34
Bpu10I CC'TnA_GC
Cuts at: 2421 2760 2421
Size: 339 6820
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2693 2982 3146 4448 5284 6149 6447 2693
Size: 289 164 1302 836 865 298 3405
Fragments arranged by size:
3405 1302 865 836 298 289 164
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3650 3695 5822 882
Size: 617 2151 45 2127 2219
Fragments arranged by size:
2219 2151 2127 617 45
BsaAI yAC'GTr
Cuts at: 493 1204 4456 4950 493
Size: 711 3252 494 2702
Fragments arranged by size:
3252 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2523 7153 2523
Size: 4630 2529
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4966 5348 5983 275
Size: 53 83 186 936 3433 382 635 1451
Fragments arranged by size:
3433 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2613 5592 5990 6264 6411 6822 2613
Size: 2979 398 274 147 411 2950
Fragments arranged by size:
2979 2950 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6934
Fragments arranged by size:
6934 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3205 3407 1186
Size: 2019 202 4938
Fragments arranged by size:
4938 2019 202
BseYI C'CCAG_C
Cuts at: 4108 6362 6858 4108
Size: 2254 496 4409
Fragments arranged by size:
4409 2254 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2191 3497 3951 1163
Size: 573 455 1306 454 4371
Fragments arranged by size:
4371 1306 573 455 454
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4609 5370 6398 1540
Size: 275 186 2608 761 1028 2301
Fragments arranged by size:
2608 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3432 3488 3816 5220 5305 6376 3432
Size: 56 328 1404 85 1071 4215
Fragments arranged by size:
4215 1404 1071 328 85 56
BsmI GAATG_Cn'
Cuts at: 4670 4763 4670
Size: 93 7066
BsmBI CGTCTCn'nnnn_
Cuts at: 3516 3516
Size: 7159
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2766 3432 3488 3816 4478 5220 5305
Size: 399 976 666 56 328 662 742 85
Cuts at: 5305 6376 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 976 742 666 662 399 328
85 56
BspEI T'CCGG_A
Cuts at: 2613 6822 2613
Size: 4209 2950
BspHI T'CATG_A
Cuts at: 5048 6778 5048
Size: 1730 5429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 2629 3392 4514 844
Size: 1319 466 763 1122 3489
Fragments arranged by size:
3489 1319 1122 763 466
BsrBI CCG'CTC
Cuts at: 1394 1823 5046 1394
Size: 429 3223 3507
Fragments arranged by size:
3507 3223 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4059 5654 5828 65
Size: 1183 2811 1595 174 1396
Fragments arranged by size:
2811 1595 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5803 5990 1700
Size: 110 3993 187 2869
Fragments arranged by size:
3993 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7159
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7159
BssSI C'ACGA_G
Cuts at: 3518 5219 6231 3518
Size: 1701 1012 4446
Fragments arranged by size:
4446 1701 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3427 794
Size: 2633 4526
BstBI TT'CG_AA
Cuts at: 2007 5963 2007
Size: 3956 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7159
BstYI r'GATC_y
Cuts at: 1972 3087 4396 4489 4856 5241 5258 6710 6796
Size: 1115 1309 93 367 385 17 1452 86
Cuts at: 6796 6808 7154 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1309 1115 385 367 346 93
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3895 3895
Size: 7159
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6607
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6420
Fragments arranged by size:
6420 683 56
BtsI GCAGTG_nn'
Cuts at: 2212 3816 4044 4096 4675 5469 5489 2212
Size: 1604 228 52 579 794 20 3882
Fragments arranged by size:
3882 1604 794 579 228 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7159
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3649 3684 529
Size: 35 3085 35 4004
Fragments arranged by size:
4004 3085 35 35
DraI TTT'AAA
Cuts at: 4463 4463
Size: 7159
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4160 6166 809
Size: 507 2844 2006 1802
Fragments arranged by size:
2844 2006 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4606 5495 7124
Size: 54 1425 50 275 18 2776 889 1629
Cuts at: 7124 8
Size: 43
Fragments arranged by size:
2776 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4606 1537
Size: 275 2794 4090
Fragments arranged by size:
4090 2794 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2346 3573 3768 4228 5089 1353
Size: 993 1227 195 460 861 3423
Fragments arranged by size:
3423 1227 993 861 460 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5746 6120 6266 387
Size: 1064 138 4157 374 146 1280
Fragments arranged by size:
4157 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3486 3486
Size: 7159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2601 2715 2946 3183 3479 3513 3575
Size: 393 969 114 231 237 296 34 62
Cuts at: 3575 3633 4069 4194 4209 5222 5819 6606 6874
Size: 58 436 125 15 1013 597 787 268
Cuts at: 6874 1239
Size: 1524
Fragments arranged by size:
1524 1013 969 787 597 436 393 296
268 237 231 125 114 62 58 34
15
EcoNI CCTnn'n_nnAGG
Cuts at: 2031 2031
Size: 7159
EcoO109I rG'GnC_Cy
Cuts at: 2774 3173 3247 2774
Size: 399 74 6686
Fragments arranged by size:
6686 399 74
EcoRI G'AATT_C
Cuts at: 4468 4846 4468
Size: 378 6781
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3131 4436 3131
Size: 1305 5854
FspI TGC'GCA
Cuts at: 5665 5665
Size: 7159
HaeII r_GCGC'y
Cuts at: 1678 2155 6306 1678
Size: 477 4151 2531
Fragments arranged by size:
4151 2531 477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2800 2832 3657 3689 3764
Size: 32 135 32 1228 32 825 32 75
Cuts at: 3764 3796 4142 4174 5800 5832 5874 5906 1373
Size: 32 346 32 1626 32 42 32 2626
Fragments arranged by size:
2626 1626 1228 825 346 135 75 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3670 4503 4749 669
Size: 988 281 1732 833 246 3079
Fragments arranged by size:
3079 1732 988 833 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7159
HpaI GTT'AAC
Cuts at: 4749 4749
Size: 7159
KpnI G_GTAC'C
Cuts at: 4484 4484
Size: 7159
MfeI C'AATT_G
Cuts at: 4758 4758
Size: 7159
MluI A'CGCG_T
Cuts at: 7035 7035
Size: 7159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4235 6273 6457 1305
Size: 2930 2038 184 2007
Fragments arranged by size:
2930 2038 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5337
Fragments arranged by size:
5337 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3821 5117 5476 5635 518
Size: 648 2655 1296 359 159 2042
Fragments arranged by size:
2655 2042 1296 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2425 3038 4067 4124 4575 5252 6400
Size: 479 340 613 1029 57 451 677 1148
Cuts at: 6400 6645 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1029 677 613 479 451 340
245 57
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6607
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7159
NheI G'CTAG_C
Cuts at: 1052 2366 1052
Size: 1314 5845
NotI GC'GGCC_GC
Cuts at: 4606 4606
Size: 7159
NspI r_CATG'y
Cuts at: 1132 1229 2070 3034 3724 4517 6062 1132
Size: 97 841 964 690 793 1545 2229
Fragments arranged by size:
2229 1545 964 841 793 690 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7159
PciI A'CATG_T
Cuts at: 2066 6058 2066
Size: 3992 3167
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3880 1108
Size: 2772 4387
PmeI GTTT'AAAC
Cuts at: 4463 4463
Size: 7159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5228 5260 6741 6773 5228
Size: 32 1481 32 5614
Fragments arranged by size:
5614 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2774 3173 3247 2774
Size: 399 74 6686
Fragments arranged by size:
6686 399 74
PshAI GACnn'nnGTC
Cuts at: 1613 2609 1613
Size: 996 6163
PsiI TTA'TAA
Cuts at: 4729 4729
Size: 7159
PstI C_TGCA'G
Cuts at: 830 2503 2626 3403 4511 830
Size: 1673 123 777 1108 3478
Fragments arranged by size:
3478 1673 1108 777 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7159
PvuII CAG'CTG
Cuts at: 2085 2425 3038 4124 2085
Size: 340 613 1086 5120
Fragments arranged by size:
5120 1086 613 340
SacI G_AGCT'C
Cuts at: 3488 3488
Size: 7159
SalI G'TCGA_C
Cuts at: 1936 4501 1936
Size: 2565 4594
SbfI CC_TGCA'GG
Cuts at: 3403 4511 3403
Size: 1108 6051
ScaI AGT'ACT
Cuts at: 5407 7119 5407
Size: 1712 5447
SfcI C'TryA_G
Cuts at: 826 1045 2499 2622 3399 4097 4507 5642 6323
Size: 219 1454 123 777 698 410 1135 681
Cuts at: 6323 6514 826
Size: 191 1471
Fragments arranged by size:
1471 1454 1135 777 698 681 410 219
191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7159
SmaI CCC'GGG
Cuts at: 1872 4486 1872
Size: 2614 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2672 2997 3014 3161 4427 5263
Size: 197 926 729 325 17 147 1266 836
Cuts at: 5263 6164 6426 820
Size: 901 262 1553
Fragments arranged by size:
1553 1266 926 901 836 729 325 262
197 147 17
SnaBI TAC'GTA
Cuts at: 493 4456 4950 493
Size: 3963 494 2702
Fragments arranged by size:
3963 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7159
SphI G_CATG'C
Cuts at: 1132 4517 1132
Size: 3385 3774
SspI AAT'ATT
Cuts at: 5 52 5083 5
Size: 47 5031 2081
Fragments arranged by size:
5031 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7159
StyI C'CwwG_G
Cuts at: 513 1065 2844 3942 4598 513
Size: 552 1779 1098 656 3074
Fragments arranged by size:
3074 1779 1098 656 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3379 5186 5203 5356 5541 5986 325
Size: 1255 1799 1807 17 153 185 445 1498
Fragments arranged by size:
1807 1799 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5405 7117 96
Size: 274 80 33 51 1443 3428 1712 138
Fragments arranged by size:
3428 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2586 2618 7119 7151 2586
Size: 32 4501 32 2594
Fragments arranged by size:
4501 2594 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7159
XbaI T'CTAG_A
Cuts at: 4495 4495
Size: 7159
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7159
XmaI C'CCGG_G
Cuts at: 1870 4484 1870
Size: 2614 4545
XmnI GAAnn'nnTTC
Cuts at: 2460 2887 3111 3114 5288 2460
Size: 427 224 3 2174 4331
Fragments arranged by size:
4331 2174 427 224 3
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4967 276
Size: 53 83 186 936 3433 2468
Fragments arranged by size:
3433 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI