Restriction map for
pFN21ASDA0661
(Circular) (Six-base) MAPSORT of: pFN21ASDA0661.seq Check: 2660 from: 1 to: 7732
pFN21ASDA0661 7732 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3300 4248 3300
Size: 948 6784
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5542 278
Size: 53 83 186 936 4006 2468
Fragments arranged by size:
4006 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4803 5075 1937
Size: 2866 272 4594
Fragments arranged by size:
4594 2866 272
Acc65I G'GTAC_C
Cuts at: 5053 5053
Size: 7732
AclI AA'CG_TT
Cuts at: 1563 5859 6232 1563
Size: 4296 373 3063
Fragments arranged by size:
4296 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2364 3086 3732 3798 4661 5795 7179
Size: 557 175 722 646 66 863 1134 1384
Cuts at: 7179 7447 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1134 863 722 646 557 268
175 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7535
AflIII A'CryG_T
Cuts at: 1203 2417 6631 7608 1203
Size: 1214 4214 977 1327
Fragments arranged by size:
4214 1327 1214 977
AgeI A'CCGG_T
Cuts at: 6563 6563
Size: 7732
AhdI GACnn_n'nnGTC
Cuts at: 6461 6461
Size: 7732
AleI CACnn'nnGTG
Cuts at: 1166 3749 1166
Size: 2583 5149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7732
AlwNI CAG_nnn'CTG
Cuts at: 2387 3055 3430 3683 3725 4498 4513 5475 7047
Size: 668 375 253 42 773 15 962 1572
Cuts at: 7047 2387
Size: 3072
Fragments arranged by size:
3072 1572 962 773 668 375 253 42
15
ApaI G_GGCC'C
Cuts at: 2073 2320 3456 3583 3811 3818 2073
Size: 247 1136 127 228 7 5987
Fragments arranged by size:
5987 1136 247 228 127 7
ApaLI G'TGCA_C
Cuts at: 5789 6945 5789
Size: 1156 6576
ApoI r'AATT_y
Cuts at: 1493 5041 5267 5419 7528 1493
Size: 3548 226 152 2109 1697
Fragments arranged by size:
3548 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6286 160
Size: 872 5254 1606
Fragments arranged by size:
5254 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7732
AvaI C'yCGr_G
Cuts at: 1870 1943 2712 3258 3825 4547 5057 1870
Size: 73 769 546 567 722 510 4545
Fragments arranged by size:
4545 769 722 567 546 510 73
AvrII C'CTAG_G
Cuts at: 3886 3886
Size: 7732
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2165 2198 2165
Size: 33 7699
BamHI G'GATC_C
Cuts at: 5062 5429 5062
Size: 367 7365
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2392 2531 3728 4256 4994
Size: 325 217 627 605 139 1197 528 738
Cuts at: 4994 5053 6508 618
Size: 59 1455 1842
Fragments arranged by size:
1842 1455 1197 738 627 605 528 325
217 139 59
BanII G_rGCy'C
Cuts at: 1391 2066 2073 2127 2320 2685 2805 2972 3456
Size: 675 7 54 193 365 120 167 484
Cuts at: 3456 3583 3811 3818 3999 4596 4747 5051 1391
Size: 127 228 7 181 597 151 304 4072
Fragments arranged by size:
4072 675 597 484 365 304 228 193
181 167 151 127 120 54 7 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2108 2370 3245 3482 928
Size: 971 209 262 875 237 5178
Fragments arranged by size:
5178 971 875 262 237 209
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 5923 5957 1903
Size: 34 2228 34 1724 34 3678
Fragments arranged by size:
3678 2228 1724 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5624 6834 5624
Size: 1210 6522
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7732
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4628 6343 136
Size: 107 122 71 631 605 2956 1715 1525
Fragments arranged by size:
2956 1715 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7727 7727
Size: 7732
BlpI GC'TnA_GC
Cuts at: 5149 5149
Size: 7732
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2320 3456 3583 3649 3811 3818 4973
Size: 283 247 1136 127 66 162 7 1155
Cuts at: 4973 4999 5793 6949 1790
Size: 26 794 1156 2573
Fragments arranged by size:
2573 1156 1155 1136 794 283 247 162
127 66 26 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2323 2537 5463
Size: 416 53 83 399 24 906 214 2926
Cuts at: 5463 6421 442
Size: 958 1753
Fragments arranged by size:
2926 1753 958 906 416 399 214 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7732
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4774 4013
Size: 761 6971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 2789 2861 3101 3386 3702 4535 4610
Size: 1340 210 72 240 285 316 833 75
Cuts at: 4610 4673 4811 6392 1239
Size: 63 138 1581 2579
Fragments arranged by size:
2579 1581 1340 833 316 285 240 210
138 75 72 63
Bpu10I CC'TnA_GC
Cuts at: 2065 2700 4110 4746 2065
Size: 635 1410 636 5051
Fragments arranged by size:
5051 1410 636 635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3116 3901 3962 3983 4457 4721 5857 6722 7020
Size: 785 61 21 474 264 1136 865 298
Cuts at: 7020 3116
Size: 3828
Fragments arranged by size:
3828 1136 865 785 474 298 264 61
21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2457 2822 3989 6395 882
Size: 617 958 365 1167 2406 2219
Fragments arranged by size:
2406 2219 1167 958 617 365
BsaAI yAC'GTr
Cuts at: 493 1204 4660 5029 5523 493
Size: 711 3456 369 494 2702
Fragments arranged by size:
3456 2702 711 494 369
BsaBI GATnn'nnATC
Cuts at: 7726 7726
Size: 7732
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3522 3639 5539 5921
Size: 53 83 186 936 1989 117 1900 382
Cuts at: 5921 6556 275
Size: 635 1451
Fragments arranged by size:
1989 1900 1451 936 635 382 186 117
83 53
BsaWI w'CCGG_w
Cuts at: 6165 6563 6837 6984 7395 6165
Size: 398 274 147 411 6502
Fragments arranged by size:
6502 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7507
Fragments arranged by size:
7507 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2171 2285 2421 2561 2962 3173 3680
Size: 925 60 114 136 140 401 211 507
Cuts at: 3680 3776 3965 4169 4238 4286 4289 4451 4532
Size: 96 189 204 69 48 3 162 81
Cuts at: 4532 4847 1186
Size: 315 4071
Fragments arranged by size:
4071 925 507 401 315 211 204 189
162 140 136 114 96 81 69 60
48 3
BseYI C'CCAG_C
Cuts at: 2319 2719 3243 3325 3525 3649 3881 4252 4491
Size: 400 524 82 200 124 232 371 239
Cuts at: 4491 4623 6935 7431 2319
Size: 132 2312 496 2620
Fragments arranged by size:
2620 2312 524 496 400 371 239 232
200 132 124 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4103 4526 4667 4682 1163
Size: 573 2367 423 141 15 4213
Fragments arranged by size:
4213 2367 573 423 141 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5182 5943 6971 1540
Size: 275 186 3181 761 1028 2301
Fragments arranged by size:
3181 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2805 2972 3309 3999 4596 4659 5793 5878 6949
Size: 167 337 690 597 63 1134 85 1071
Cuts at: 6949 2805
Size: 3588
Fragments arranged by size:
3588 1134 1071 690 597 337 167 85
63
BsmI GAATG_Cn'
Cuts at: 3034 4209 5243 5336 3034
Size: 1175 1034 93 5430
Fragments arranged by size:
5430 1175 1034 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3167 3167
Size: 7732
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2073 2127 2320 2685 2805 2972
Size: 399 276 7 54 193 365 120 167
Cuts at: 2972 3309 3456 3583 3649 3811 3818 3999 4596
Size: 337 147 127 66 162 7 181 597
Cuts at: 4596 4659 4747 4973 4999 5051 5793 5878 6949
Size: 63 88 226 26 52 742 85 1071
Cuts at: 6949 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 597 399 365 337 276
226 193 181 167 162 147 127 120
88 85 66 63 54 52 26 7
7
BspEI T'CCGG_A
Cuts at: 7395 7395
Size: 7732
BspHI T'CATG_A
Cuts at: 5621 7351 5621
Size: 1730 6002
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3300 4248 4450 4871 5087 844
Size: 2456 948 202 421 216 3489
Fragments arranged by size:
3489 2456 948 421 216 202
BsrBI CCG'CTC
Cuts at: 1394 1823 2206 3911 4399 5619 1394
Size: 429 383 1705 488 1220 3507
Fragments arranged by size:
3507 1705 1220 488 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 6227 6401 65
Size: 1183 4979 174 1396
Fragments arranged by size:
4979 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 2503 2733 6376 6563 1700
Size: 110 241 452 230 3643 187 2869
Fragments arranged by size:
3643 2869 452 241 230 187 110
BsrGI T'GTAC_A
Cuts at: 96 2661 4047 96
Size: 2565 1386 3781
Fragments arranged by size:
3781 2565 1386
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7732
BssSI C'ACGA_G
Cuts at: 2601 4713 5792 6804 2601
Size: 2112 1079 1012 3529
Fragments arranged by size:
3529 2112 1079 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2531 794
Size: 1737 5995
BstBI TT'CG_AA
Cuts at: 2007 6536 2007
Size: 4529 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7732
BstXI CCAn_nnnn'nTGG
Cuts at: 2839 3590 2839
Size: 751 6981
BstYI r'GATC_y
Cuts at: 1972 4680 5062 5429 5814 5831 7283 7369 7381
Size: 2708 382 367 385 17 1452 86 12
Cuts at: 7381 7727 1972
Size: 346 1977
Fragments arranged by size:
2708 1977 1452 385 382 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2388 2974 3156 3840 2388
Size: 586 182 684 6280
Fragments arranged by size:
6280 684 586 182
BtgI C'CryG_G
Cuts at: 513 1065 2151 513
Size: 552 1086 6094
Fragments arranged by size:
6094 1086 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6993
Fragments arranged by size:
6993 683 56
BtsI GCAGTG_nn'
Cuts at: 2548 2876 3209 5248 6042 6062 2548
Size: 328 333 2039 794 20 4218
Fragments arranged by size:
4218 2039 794 333 328 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7732
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7697
DraI TTT'AAA
Cuts at: 5036 5036
Size: 7732
DraIII CAC_nnn'GTG
Cuts at: 2531 2531
Size: 7732
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6739 809
Size: 507 5423 1802
Fragments arranged by size:
5423 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2751 3057 3273
Size: 54 1425 50 275 18 921 306 216
Cuts at: 3273 3435 4629 5179 6068 7697 8
Size: 162 1194 550 889 1629 43
Fragments arranged by size:
1629 1425 1194 921 889 550 306 275
216 162 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5179 1537
Size: 275 3367 4090
Fragments arranged by size:
4090 3367 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2152 3774 5662 1353
Size: 799 1622 1888 3423
Fragments arranged by size:
3423 1888 1622 799
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6319 6693 6839 387
Size: 1064 138 4730 374 146 1280
Fragments arranged by size:
4730 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2803 2970 3997 4594 2803
Size: 167 1027 597 5941
Fragments arranged by size:
5941 1027 597 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2364 2579 2789 2861 3086 3101
Size: 393 557 175 215 210 72 225 15
Cuts at: 3101 3386 3702 3732 3798 4535 4610 4661 4673
Size: 285 316 30 66 737 75 51 12
Cuts at: 4673 4811 5795 6392 7179 7447 1239
Size: 138 984 597 787 268 1524
Fragments arranged by size:
1524 984 787 737 597 557 393 316
285 268 225 215 210 175 138 75
72 66 51 30 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2690 2924 3155 4829 2690
Size: 234 231 1674 5593
Fragments arranged by size:
5593 1674 234 231
EcoO109I rG'GnC_Cy
Cuts at: 2069 2070 2400 3262 3452 3680 3807 3814 3815
Size: 1 330 862 190 228 127 7 1
Cuts at: 3815 3878 3890 4462 4636 2069
Size: 63 12 572 174 5165
Fragments arranged by size:
5165 862 572 330 228 190 174 127
63 12 7 1 1
EcoRI G'AATT_C
Cuts at: 5041 5419 5041
Size: 378 7354
FspI TGC'GCA
Cuts at: 3410 4577 6238 3410
Size: 1167 1661 4904
Fragments arranged by size:
4904 1661 1167
HaeII r_GCGC'y
Cuts at: 1678 2387 2635 3130 4061 6879 1678
Size: 709 248 495 931 2818 2531
Fragments arranged by size:
2818 2531 931 709 495 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 3472 3504 6373
Size: 32 135 32 476 32 1392 32 2869
Cuts at: 6373 6405 6447 6479 1373
Size: 32 42 32 2626
Fragments arranged by size:
2869 2626 1392 476 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4860 5076 5322 669
Size: 988 281 2922 216 246 3079
Fragments arranged by size:
3079 2922 988 281 246 216
HindIII A'AGCT_T
Cuts at: 748 3542 748
Size: 2794 4938
HpaI GTT'AAC
Cuts at: 5322 5322
Size: 7732
KpnI G_GTAC'C
Cuts at: 5057 5057
Size: 7732
MfeI C'AATT_G
Cuts at: 5331 5331
Size: 7732
MluI A'CGCG_T
Cuts at: 7608 7608
Size: 7732
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 6846 7030 1305
Size: 936 4605 184 2007
Fragments arranged by size:
4605 2007 936 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2753 3059 3275 10
Size: 54 1425 343 921 306 216 4467
Fragments arranged by size:
4467 1425 921 343 306 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3749 5690 6049 6208 518
Size: 648 2583 1941 359 159 2042
Fragments arranged by size:
2583 2042 1941 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2323 2482 2594 2761 3364 4072 4097 4495
Size: 717 159 112 167 603 708 25 398
Cuts at: 4495 4627 4651 5148 5825 6973 7218 1606
Size: 132 24 497 677 1148 245 2120
Fragments arranged by size:
2120 1148 717 708 677 603 497 398
245 167 159 132 112 25 24
NaeI GCC'GGC
Cuts at: 2053 2505 2053
Size: 452 7280
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7180
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7732
NgoMIV G'CCGG_C
Cuts at: 2051 2503 2051
Size: 452 7280
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7732
NotI GC'GGCC_GC
Cuts at: 5179 5179
Size: 7732
NspI r_CATG'y
Cuts at: 1132 1229 2421 3010 3193 4327 5090 6635 1132
Size: 97 1192 589 183 1134 763 1545 2229
Fragments arranged by size:
2229 1545 1192 1134 763 589 183 97
PasI CC'CwG_GG
Cuts at: 1348 3656 3803 3811 1348
Size: 2308 147 8 5269
Fragments arranged by size:
5269 2308 147 8
PciI A'CATG_T
Cuts at: 2417 6631 2417
Size: 4214 3518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3055 3725 4588 1108
Size: 1947 670 863 4252
Fragments arranged by size:
4252 1947 863 670
PfoI T'CCnGG_A
Cuts at: 2805 4143 4827 2805
Size: 1338 684 5710
Fragments arranged by size:
5710 1338 684
PmeI GTTT'AAAC
Cuts at: 5036 5036
Size: 7732
PmlI CAC'GTG
Cuts at: 4660 4660
Size: 7732
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5801 5833 7314 7346 5801
Size: 32 1481 32 6187
Fragments arranged by size:
6187 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2400 3878 3890 4462 4636 2400
Size: 1478 12 572 174 5496
Fragments arranged by size:
5496 1478 572 174 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7732
PsiI TTA'TAA
Cuts at: 5302 5302
Size: 7732
PspOMI G'GGCC_C
Cuts at: 2069 2316 3452 3579 3807 3814 2069
Size: 247 1136 127 228 7 5987
Fragments arranged by size:
5987 1136 247 228 127 7
PstI C_TGCA'G
Cuts at: 830 2745 2766 3093 3198 3336 4245 5084 830
Size: 1915 21 327 105 138 909 839 3478
Fragments arranged by size:
3478 1915 909 839 327 138 105 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7732
PvuII CAG'CTG
Cuts at: 2323 2482 2594 2761 3364 4495 4627 4651 2323
Size: 159 112 167 603 1131 132 24 5404
Fragments arranged by size:
5404 1131 603 167 159 132 112 24
SacI G_AGCT'C
Cuts at: 2805 2972 3999 4596 2805
Size: 167 1027 597 5941
Fragments arranged by size:
5941 1027 597 167
SalI G'TCGA_C
Cuts at: 1936 5074 1936
Size: 3138 4594
SanDI GG'GwC_CC
Cuts at: 2400 2400
Size: 7732
SbfI CC_TGCA'GG
Cuts at: 5084 5084
Size: 7732
ScaI AGT'ACT
Cuts at: 2795 5980 7692 2795
Size: 3185 1712 2835
Fragments arranged by size:
3185 2835 1712
SfcI C'TryA_G
Cuts at: 826 1045 2177 2627 2741 2762 3089 3194 3332
Size: 219 1132 450 114 21 327 105 138
Cuts at: 3332 3900 4241 4349 4611 5080 6215 6896 7087
Size: 568 341 108 262 469 1135 681 191
Cuts at: 7087 826
Size: 1471
Fragments arranged by size:
1471 1135 1132 681 568 469 450 341
327 262 219 191 138 114 108 105
21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7732
SgrAI Cr'CCGG_yG
Cuts at: 1700 2051 1700
Size: 351 7381
SmaI CCC'GGG
Cuts at: 1872 3827 5059 1872
Size: 1955 1232 4545
Fragments arranged by size:
4545 1955 1232
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3131 3916 3977 3998 4472 4736
Size: 197 926 1188 785 61 21 474 264
Cuts at: 4736 5836 6737 6999 820
Size: 1100 901 262 1553
Fragments arranged by size:
1553 1188 1100 926 901 785 474 264
262 197 61 21
SnaBI TAC'GTA
Cuts at: 493 5029 5523 493
Size: 4536 494 2702
Fragments arranged by size:
4536 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7732
SphI G_CATG'C
Cuts at: 1132 3010 3193 5090 1132
Size: 1878 183 1897 3774
Fragments arranged by size:
3774 1897 1878 183
SspI AAT'ATT
Cuts at: 5 52 5656 5
Size: 47 5604 2081
Fragments arranged by size:
5604 2081 47
StuI AGG'CCT
Cuts at: 1517 2615 2930 1517
Size: 1098 315 6319
Fragments arranged by size:
6319 1098 315
StyI C'CwwG_G
Cuts at: 513 1065 2508 2586 2832 3886 3939 4062 4465
Size: 552 1443 78 246 1054 53 123 403
Cuts at: 4465 5171 513
Size: 706 3074
Fragments arranged by size:
3074 1443 1054 706 552 403 246 123
78 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2519 3639 4244 5759 5776 5929 6114
Size: 1255 939 1120 605 1515 17 153 185
Cuts at: 6114 6559 325
Size: 445 1498
Fragments arranged by size:
1515 1498 1255 1120 939 605 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2661 2793 3553
Size: 274 80 33 51 1443 684 132 760
Cuts at: 3553 4047 4845 5978 7690 96
Size: 494 798 1133 1712 138
Fragments arranged by size:
1712 1443 1133 798 760 684 494 274
138 132 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2855 2887 7692 7724 2855
Size: 32 4805 32 2863
Fragments arranged by size:
4805 2863 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7732
XbaI T'CTAG_A
Cuts at: 5068 5068
Size: 7732
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2158 3067 3613 2158
Size: 909 546 6277
Fragments arranged by size:
6277 909 546
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7732
XmaI C'CCGG_G
Cuts at: 1870 3825 5057 1870
Size: 1955 1232 4545
Fragments arranged by size:
4545 1955 1232
XmnI GAAnn'nnTTC
Cuts at: 2343 5861 2343
Size: 3518 4214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5540 276
Size: 53 83 186 936 4006 2468
Fragments arranged by size:
4006 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BbvCI BfrBI BsiWI BstZ17I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PspXI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI