Restriction map for
pFN21ASDA0659
(Circular) (Six-base) MAPSORT of: pFN21ASDA0659.seq Check: 3663 from: 1 to: 7855
pFN21ASDA0659 7855 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3981 3981
Size: 7855
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5665 278
Size: 53 83 186 936 4129 2468
Fragments arranged by size:
4129 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4304 5198 1937
Size: 2367 894 4594
Fragments arranged by size:
4594 2367 894
Acc65I G'GTAC_C
Cuts at: 5176 5176
Size: 7855
AclI AA'CG_TT
Cuts at: 1563 5982 6355 1563
Size: 4419 373 3063
Fragments arranged by size:
4419 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5918 7302 7570 1632
Size: 4286 1384 268 1917
Fragments arranged by size:
4286 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7658
AflIII A'CryG_T
Cuts at: 1203 6754 7731 1203
Size: 5551 977 1327
Fragments arranged by size:
5551 1327 977
AgeI A'CCGG_T
Cuts at: 6686 6686
Size: 7855
AhdI GACnn_n'nnGTC
Cuts at: 2653 4381 4558 6584 2653
Size: 1728 177 2026 3924
Fragments arranged by size:
3924 2026 1728 177
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7855
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2798 3886 4556 4850 1184
Size: 1614 1088 670 294 4189
Fragments arranged by size:
4189 1614 1088 670 294
AlwNI CAG_nnn'CTG
Cuts at: 3646 4588 4753 5598 7170 3646
Size: 942 165 845 1572 4331
Fragments arranged by size:
4331 1572 942 845 165
ApaI G_GGCC'C
Cuts at: 4871 4871
Size: 7855
ApaLI G'TGCA_C
Cuts at: 5912 7068 5912
Size: 1156 6699
ApoI r'AATT_y
Cuts at: 1493 2866 4757 5164 5390 5542 7651 1493
Size: 1373 1891 407 226 152 2109 1697
Fragments arranged by size:
2109 1891 1697 1373 407 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6409 160
Size: 872 5377 1606
Fragments arranged by size:
5377 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7855
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 4505 4838 4972 5011 5180 1870
Size: 73 84 2478 333 134 39 169 4545
Fragments arranged by size:
4545 2478 333 169 134 84 73 39
BamHI G'GATC_C
Cuts at: 5185 5552 5185
Size: 367 7488
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2571 3087 3285 3293 4345
Size: 325 217 627 784 516 198 8 1052
Cuts at: 4345 4722 4834 4927 5176 6631 618
Size: 377 112 93 249 1455 1842
Fragments arranged by size:
1842 1455 1052 784 627 516 377 325
249 217 198 112 93 8
BanII G_rGCy'C
Cuts at: 1391 2292 3897 3999 4070 4098 4439 4529 4847
Size: 901 1605 102 71 28 341 90 318
Cuts at: 4847 4871 4920 5022 5174 1391
Size: 24 49 102 152 4072
Fragments arranged by size:
4072 1605 901 341 318 152 102 102
90 71 49 28 24
BbeI G_GCGC'C
Cuts at: 3091 4726 3091
Size: 1635 6220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2482 3057 4037 928
Size: 971 583 575 980 4746
Fragments arranged by size:
4746 980 971 583 575
BbvCI CC'TCA_GC
Cuts at: 4863 4863
Size: 7855
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2393 2427 2714 2748 3577 3611 6046
Size: 34 456 34 287 34 829 34 2435
Cuts at: 6046 6080 1903
Size: 34 3678
Fragments arranged by size:
3678 2435 829 456 287 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3221 3498 5747 6957 3221
Size: 277 2249 1210 4119
Fragments arranged by size:
4119 2249 1210 277
BclI T'GATC_A
Cuts at: 1548 3204 3942 1548
Size: 1656 738 5461
Fragments arranged by size:
5461 1656 738
BfrBI ATG'CAT
Cuts at: 3708 3708
Size: 7855
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3679 4224 4343
Size: 107 122 71 631 605 2007 545 119
Cuts at: 4343 4942 6466 136
Size: 599 1524 1525
Fragments arranged by size:
2007 1525 1524 631 605 599 545 122
119 107 71
BglII A'GATC_T
Cuts at: 7850 7850
Size: 7855
BlpI GC'TnA_GC
Cuts at: 2222 2258 3264 4957 5003 5272 2222
Size: 36 1006 1693 46 269 4805
Fragments arranged by size:
4805 1693 1006 269 46 36
Bme1580I G_kGCm'C
Cuts at: 1790 3288 3452 4348 4839 4871 4909 4932 5916
Size: 1498 164 896 491 32 38 23 984
Cuts at: 5916 7072 1790
Size: 1156 2573
Fragments arranged by size:
2573 1498 1156 984 896 491 164 38
32 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7855
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2458 2647 4198
Size: 416 53 83 399 24 1041 189 1551
Cuts at: 4198 4629 4734 4903 5586 6544 442
Size: 431 105 169 683 958 1753
Fragments arranged by size:
1753 1551 1041 958 683 431 416 399
189 169 105 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7855
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 4246 4387 2191
Size: 2055 141 5659
Fragments arranged by size:
5659 2055 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3054 3371 4031 4175 4241 4481 4610 6515
Size: 1815 317 660 144 66 240 129 1905
Cuts at: 6515 1239
Size: 2579
Fragments arranged by size:
2579 1905 1815 660 317 240 144 129
66
Bpu10I CC'TnA_GC
Cuts at: 4092 4523 4623 4863 4092
Size: 431 100 240 7084
Fragments arranged by size:
7084 431 240 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2846 3482 5980 6845 7143 2846
Size: 636 2498 865 298 3558
Fragments arranged by size:
3558 2498 865 636 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3381 6518 882
Size: 617 1882 3137 2219
Fragments arranged by size:
3137 2219 1882 617
BsaAI yAC'GTr
Cuts at: 493 1204 2311 2320 3172 3844 5152 5646 493
Size: 711 1107 9 852 672 1308 494 2702
Fragments arranged by size:
2702 1308 1107 852 711 672 494 9
BsaBI GATnn'nnATC
Cuts at: 2246 3209 7849 2246
Size: 963 4640 2252
Fragments arranged by size:
4640 2252 963
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3088 4614 4723 5033
Size: 53 83 186 936 1555 1526 109 310
Cuts at: 5033 5662 6044 6679 275
Size: 629 382 635 1451
Fragments arranged by size:
1555 1526 1451 936 635 629 382 310
186 109 83 53
BsaWI w'CCGG_w
Cuts at: 5079 6288 6686 6960 7107 7518 5079
Size: 1209 398 274 147 411 5416
Fragments arranged by size:
5416 1209 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2378 2408 2530 2560 1178
Size: 30 165 30 975 30 122 30 6473
Fragments arranged by size:
6473 975 165 122 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2551 2597 3746 3905 4402 4670 4960 4999
Size: 1365 46 1149 159 497 268 290 39
Cuts at: 4999 1186
Size: 4042
Fragments arranged by size:
4042 1365 1149 497 290 268 159 46
39
BseYI C'CCAG_C
Cuts at: 3301 3779 4442 4880 7058 7554 3301
Size: 478 663 438 2178 496 3602
Fragments arranged by size:
3602 2178 663 496 478 438
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2617 4697 1163
Size: 573 881 2080 4321
Fragments arranged by size:
4321 2080 881 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2200 3832 4551 5305 6066 7094
Size: 275 186 199 1632 719 754 761 1028
Cuts at: 7094 1540
Size: 2301
Fragments arranged by size:
2301 1632 1028 761 754 719 275 199
186
BsiHKAI G_wGCw'C
Cuts at: 2142 2316 3972 4098 4529 5022 5916 6001 7072
Size: 174 1656 126 431 493 894 85 1071
Cuts at: 7072 2142
Size: 2925
Fragments arranged by size:
2925 1656 1071 894 493 431 174 126
85
BsmI GAATG_Cn'
Cuts at: 5063 5366 5459 5063
Size: 303 93 7459
Fragments arranged by size:
7459 303 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2292 2316 3288 3452 3897 3972
Size: 399 352 150 24 972 164 445 75
Cuts at: 3972 3999 4070 4098 4348 4439 4529 4839 4847
Size: 27 71 28 250 91 90 310 8
Cuts at: 4847 4871 4909 4920 4932 5022 5174 5916 6001
Size: 24 38 11 12 90 152 742 85
Cuts at: 6001 7072 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 972 742 445 399 352 310
250 164 152 150 91 90 90 85
75 71 38 28 27 24 24 12
11 8
BspEI T'CCGG_A
Cuts at: 5079 7518 5079
Size: 2439 5416
BspHI T'CATG_A
Cuts at: 5744 7474 5744
Size: 1730 6125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2559 2574 3981 5210 844
Size: 1715 15 1407 1229 3489
Fragments arranged by size:
3489 1715 1407 1229 15
BsrBI CCG'CTC
Cuts at: 1394 1823 4037 4315 4410 4627 5002 5742 1394
Size: 429 2214 278 95 217 375 740 3507
Fragments arranged by size:
3507 2214 740 429 375 278 217 95
BsrDI GCAATG_nn'
Cuts at: 65 1248 2730 3075 3552 6350 6524 65
Size: 1183 1482 345 477 2798 174 1396
Fragments arranged by size:
2798 1482 1396 1183 477 345 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2610 2830 4687 4952 6499 6686 1700
Size: 110 800 220 1857 265 1547 187 2869
Fragments arranged by size:
2869 1857 1547 800 265 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 4389 96
Size: 4293 3562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7855
BssSI C'ACGA_G
Cuts at: 2165 2995 5915 6927 2165
Size: 830 2920 1012 3093
Fragments arranged by size:
3093 2920 1012 830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2311 794
Size: 1517 6338
BstBI TT'CG_AA
Cuts at: 2007 6659 2007
Size: 4652 3203
BstEII G'GTnAC_C
Cuts at: 1184 2833 3742 1184
Size: 1649 909 5297
Fragments arranged by size:
5297 1649 909
BstXI CCAn_nnnn'nTGG
Cuts at: 3380 4060 3380
Size: 680 7175
BstYI r'GATC_y
Cuts at: 1972 4893 5076 5185 5552 5937 5954 7406 7492
Size: 2921 183 109 367 385 17 1452 86
Cuts at: 7492 7504 7850 1972
Size: 12 346 1977
Fragments arranged by size:
2921 1977 1452 385 367 346 183 109
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3729 4414 4746 3729
Size: 685 332 6838
Fragments arranged by size:
6838 685 332
BtgI C'CryG_G
Cuts at: 513 1065 2133 2194 2200 2499 3020 3276 3289
Size: 552 1068 61 6 299 521 256 13
Cuts at: 3289 3397 4122 4359 4696 4940 5037 5101 513
Size: 108 725 237 337 244 97 64 3267
Fragments arranged by size:
3267 1068 725 552 521 337 299 256
244 237 108 97 64 61 13 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7116
Fragments arranged by size:
7116 683 56
BtsI GCAGTG_nn'
Cuts at: 2071 2409 3450 3875 4612 5371 6165 6185 2071
Size: 338 1041 425 737 759 794 20 3741
Fragments arranged by size:
3741 1041 794 759 737 425 338 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7855
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2101 2136 3043 3078 3886 3921 4129
Size: 35 1537 35 907 35 808 35 208
Cuts at: 4129 4164 529
Size: 35 4220
Fragments arranged by size:
4220 1537 907 808 208 35 35 35
35 35
DraI TTT'AAA
Cuts at: 5159 5159
Size: 7855
DraIII CAC_nnn'GTG
Cuts at: 4456 4456
Size: 7855
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6862 809
Size: 507 5546 1802
Fragments arranged by size:
5546 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2197 2244 2328
Size: 54 1425 50 275 18 367 47 84
Cuts at: 2328 3753 3829 4353 4548 4644 5098 5302 6191
Size: 1425 76 524 195 96 454 204 889
Cuts at: 6191 7820 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1425 889 524 454 367 275
204 195 96 84 76 54 50 47
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2197 3829 4548 5302 1537
Size: 275 385 1632 719 754 4090
Fragments arranged by size:
4090 1632 754 719 385 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2147 4487 4656 4787 5785 1353
Size: 794 2340 169 131 998 3423
Fragments arranged by size:
3423 2340 998 794 169 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3697 4340 6442 6816 6962 387
Size: 1064 138 2108 643 2102 374 146 1280
Fragments arranged by size:
2108 2102 1280 1064 643 374 146 138
EcoICRI GAG'CTC
Cuts at: 4096 4527 5020 4096
Size: 431 493 6931
Fragments arranged by size:
6931 493 431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3054 3371 4031 4175 4241 4481 4610
Size: 393 1422 317 660 144 66 240 129
Cuts at: 4610 5918 6515 7302 7570 1239
Size: 1308 597 787 268 1524
Fragments arranged by size:
1524 1422 1308 787 660 597 393 317
268 240 144 129 66
EcoNI CCTnn'n_nnAGG
Cuts at: 3371 3371
Size: 7855
EcoO109I rG'GnC_Cy
Cuts at: 2704 3219 3526 4279 4867 4868 2704
Size: 515 307 753 588 1 5691
Fragments arranged by size:
5691 753 588 515 307 1
EcoRI G'AATT_C
Cuts at: 2866 4757 5164 5542 2866
Size: 1891 407 378 5179
Fragments arranged by size:
5179 1891 407 378
FseI GG_CCGG'CC
Cuts at: 4691 4691
Size: 7855
FspI TGC'GCA
Cuts at: 6361 6361
Size: 7855
HaeII r_GCGC'y
Cuts at: 1678 2053 3091 3271 4712 4726 7002 1678
Size: 375 1038 180 1441 14 2276 2531
Fragments arranged by size:
2531 2276 1441 1038 375 180 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2639 2651 2671 2683 3027
Size: 32 135 32 1067 12 20 12 344
Cuts at: 3027 3059 4367 4399 4544 4576 4955 4987 6496
Size: 32 1308 32 145 32 379 32 1509
Cuts at: 6496 6528 6570 6602 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1509 1308 1067 379 344 145 135
42 32 32 32 32 32 32 32
32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 5199 5445 669
Size: 988 281 3261 246 3079
Fragments arranged by size:
3261 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7855
HpaI GTT'AAC
Cuts at: 5445 5445
Size: 7855
KasI G'GCGC_C
Cuts at: 3087 4722 3087
Size: 1635 6220
KpnI G_GTAC'C
Cuts at: 5180 5180
Size: 7855
MfeI C'AATT_G
Cuts at: 5454 5454
Size: 7855
MluI A'CGCG_T
Cuts at: 7731 7731
Size: 7855
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2748 3515 3699 6969 7153 1305
Size: 1443 767 184 3270 184 2007
Fragments arranged by size:
3270 2007 1443 767 184 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2246 2330 3755 4355 5100
Size: 54 1425 343 414 84 1425 600 745
Cuts at: 5100 10
Size: 2765
Fragments arranged by size:
2765 1425 1425 745 600 414 343 84
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 3842 4832 5813 6172 6331 518
Size: 648 1170 1506 990 981 359 159 2042
Fragments arranged by size:
2042 1506 1170 990 981 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2202 2601 2758 2764 3022 3329 3399 3622
Size: 596 399 157 6 258 307 70 223
Cuts at: 3622 4698 5271 5948 7096 7341 1606
Size: 1076 573 677 1148 245 2120
Fragments arranged by size:
2120 1148 1076 677 596 573 399 307
258 245 223 157 70 6
NaeI GCC'GGC
Cuts at: 2612 4689 4954 2612
Size: 2077 265 5513
Fragments arranged by size:
5513 2077 265
NarI GG'CG_CC
Cuts at: 3088 4723 3088
Size: 1635 6220
NcoI C'CATG_G
Cuts at: 513 1065 4359 513
Size: 552 3294 4009
Fragments arranged by size:
4009 3294 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7855
NgoMIV G'CCGG_C
Cuts at: 2610 4687 4952 2610
Size: 2077 265 5513
Fragments arranged by size:
5513 2077 265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7855
NotI GC'GGCC_GC
Cuts at: 5302 5302
Size: 7855
NsiI A_TGCA'T
Cuts at: 3710 3710
Size: 7855
NspI r_CATG'y
Cuts at: 1132 1229 2266 2911 5213 6758 1132
Size: 97 1037 645 2302 1545 2229
Fragments arranged by size:
2302 2229 1545 1037 645 97
PasI CC'CwG_GG
Cuts at: 1348 3439 4600 1348
Size: 2091 1161 4603
Fragments arranged by size:
4603 2091 1161
PciI A'CATG_T
Cuts at: 6754 6754
Size: 7855
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2253 2601 3097 3439 1108
Size: 1145 348 496 342 5524
Fragments arranged by size:
5524 1145 496 348 342
PfoI T'CCnGG_A
Cuts at: 2700 3096 3645 2700
Size: 396 549 6910
Fragments arranged by size:
6910 549 396
PmeI GTTT'AAAC
Cuts at: 5159 5159
Size: 7855
PmlI CAC'GTG
Cuts at: 3844 3844
Size: 7855
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5924 5956 7437 7469 5924
Size: 32 1481 32 6310
Fragments arranged by size:
6310 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2704 3219 4279 2704
Size: 515 1060 6280
Fragments arranged by size:
6280 1060 515
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7855
PsiI TTA'TAA
Cuts at: 5425 5425
Size: 7855
PspOMI G'GGCC_C
Cuts at: 4867 4867
Size: 7855
PstI C_TGCA'G
Cuts at: 830 3660 3865 4051 4171 4335 5207 830
Size: 2830 205 186 120 164 872 3478
Fragments arranged by size:
3478 2830 872 205 186 164 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7855
PvuII CAG'CTG
Cuts at: 2601 3329 3622 2601
Size: 728 293 6834
Fragments arranged by size:
6834 728 293
SacI G_AGCT'C
Cuts at: 4098 4529 5022 4098
Size: 431 493 6931
Fragments arranged by size:
6931 493 431
SacII CC_GC'GG
Cuts at: 2203 3023 3400 4699 2203
Size: 820 377 1299 5359
Fragments arranged by size:
5359 1299 820 377
SalI G'TCGA_C
Cuts at: 1936 5197 1936
Size: 3261 4594
SanDI GG'GwC_CC
Cuts at: 3219 3219
Size: 7855
SapI GCTCTTCn'nnn_
Cuts at: 2147 2147
Size: 7855
SbfI CC_TGCA'GG
Cuts at: 5207 5207
Size: 7855
ScaI AGT'ACT
Cuts at: 2387 6103 7815 2387
Size: 3716 1712 2427
Fragments arranged by size:
3716 2427 1712
SexAI A'CCwGG_T
Cuts at: 2114 2184 4923 2114
Size: 70 2739 5046
Fragments arranged by size:
5046 2739 70
SfcI C'TryA_G
Cuts at: 826 1045 3656 3792 3861 4047 4167 4331 5203
Size: 219 2611 136 69 186 120 164 872
Cuts at: 5203 6338 7019 7210 826
Size: 1135 681 191 1471
Fragments arranged by size:
2611 1471 1135 872 681 219 191 186
164 136 120 69
SfoI GGC'GCC
Cuts at: 3089 4724 3089
Size: 1635 6220
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7855
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7855
SmaI CCC'GGG
Cuts at: 1872 2029 5182 1872
Size: 157 3153 4545
Fragments arranged by size:
4545 3153 157
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2861 3497 5959 6860 7122 820
Size: 197 926 918 636 2462 901 262 1553
Fragments arranged by size:
2462 1553 926 918 901 636 262 197
SnaBI TAC'GTA
Cuts at: 493 5152 5646 493
Size: 4659 494 2702
Fragments arranged by size:
4659 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7855
SphI G_CATG'C
Cuts at: 1132 2266 5213 1132
Size: 1134 2947 3774
Fragments arranged by size:
3774 2947 1134
SspI AAT'ATT
Cuts at: 5 52 5779 5
Size: 47 5727 2081
Fragments arranged by size:
5727 2081 47
StuI AGG'CCT
Cuts at: 1517 2171 3377 3929 1517
Size: 654 1206 552 5443
Fragments arranged by size:
5443 1206 654 552
StyI C'CwwG_G
Cuts at: 513 1065 3231 3558 4053 4359 5294 513
Size: 552 2166 327 495 306 935 3074
Fragments arranged by size:
3074 2166 935 552 495 327 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2976 5882 5899 6052 6237 6682 325
Size: 1255 1396 2906 17 153 185 445 1498
Fragments arranged by size:
2906 1498 1396 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 3333 4389
Size: 274 80 33 51 1443 408 948 1056
Cuts at: 4389 6101 7813 96
Size: 1712 1712 138
Fragments arranged by size:
1712 1712 1443 1056 948 408 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7815 7847 7815
Size: 32 7823
Tth111I GACn'n_nGTC
Cuts at: 1316 2075 3125 3223 3596 1316
Size: 759 1050 98 373 5575
Fragments arranged by size:
5575 1050 759 373 98
XbaI T'CTAG_A
Cuts at: 5191 5191
Size: 7855
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7855
XmaI C'CCGG_G
Cuts at: 1870 2027 5180 1870
Size: 157 3153 4545
Fragments arranged by size:
4545 3153 157
XmnI GAAnn'nnTTC
Cuts at: 5984 5984
Size: 7855
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5663 276
Size: 53 83 186 936 4129 2468
Fragments arranged by size:
4129 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I
EcoRV FalI FspAI NruI PacI PspXI PsrI RsrII
SfiI SrfI SwaI XcmI