Restriction map for
pFN21ASDA0655
(Circular) (Six-base) MAPSORT of: pFN21ASDA0655.seq Check: 434 from: 1 to: 7933
pFN21ASDA0655 7933 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3505 3972 4202 4963 3505
Size: 467 230 761 6475
Fragments arranged by size:
6475 761 467 230
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5743 278
Size: 53 83 186 936 4207 2468
Fragments arranged by size:
4207 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2418 4371 5276 1937
Size: 481 1953 905 4594
Fragments arranged by size:
4594 1953 905 481
Acc65I G'GTAC_C
Cuts at: 2343 5254 2343
Size: 2911 5022
AclI AA'CG_TT
Cuts at: 1563 6060 6433 1563
Size: 4497 373 3063
Fragments arranged by size:
4497 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2603 3194 3392 4289 4970 5996 7380 7648
Size: 971 591 198 897 681 1026 1384 268
Cuts at: 7648 1632
Size: 1917
Fragments arranged by size:
1917 1384 1026 971 897 681 591 268
198
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7736
AflIII A'CryG_T
Cuts at: 1203 6832 7809 1203
Size: 5629 977 1327
Fragments arranged by size:
5629 1327 977
AgeI A'CCGG_T
Cuts at: 2775 6764 2775
Size: 3989 3944
AhdI GACnn_n'nnGTC
Cuts at: 6662 6662
Size: 7933
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7933
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 7901
AlwNI CAG_nnn'CTG
Cuts at: 3316 3748 3856 3868 4057 4255 4321 5676 7248
Size: 432 108 12 189 198 66 1355 1572
Cuts at: 7248 3316
Size: 4001
Fragments arranged by size:
4001 1572 1355 432 198 189 108 66
12
ApaI G_GGCC'C
Cuts at: 2997 4006 4213 2997
Size: 1009 207 6717
Fragments arranged by size:
6717 1009 207
ApaLI G'TGCA_C
Cuts at: 3401 5990 7146 3401
Size: 2589 1156 4188
Fragments arranged by size:
4188 2589 1156
ApoI r'AATT_y
Cuts at: 1493 4601 5242 5468 5620 7729 1493
Size: 3108 641 226 152 2109 1697
Fragments arranged by size:
3108 2109 1697 641 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6487 160
Size: 872 5455 1606
Fragments arranged by size:
5455 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7933
AvaI C'yCGr_G
Cuts at: 1870 1943 2870 2939 4212 4459 5258 1870
Size: 73 927 69 1273 247 799 4545
Fragments arranged by size:
4545 1273 927 799 247 73 69
BamHI G'GATC_C
Cuts at: 4488 5263 5630 4488
Size: 775 367 6791
Fragments arranged by size:
6791 775 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2313 2343 4142 5254 6709
Size: 325 217 627 526 30 1799 1112 1455
Cuts at: 6709 618
Size: 1842
Fragments arranged by size:
1842 1799 1455 1112 627 526 325 217
30
BanII G_rGCy'C
Cuts at: 1391 2997 3393 3662 4006 4213 4219 4227 4731
Size: 1606 396 269 344 207 6 8 504
Cuts at: 4731 5252 1391
Size: 521 4072
Fragments arranged by size:
4072 1606 521 504 396 344 269 207
8 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5003 928
Size: 971 3104 3858
Fragments arranged by size:
3858 3104 971
BbvCI CC'TCA_GC
Cuts at: 2899 3278 2899
Size: 379 7554
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6124 6158 1903
Size: 34 4187 34 3678
Fragments arranged by size:
4187 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2641 4117 5825 7035 2641
Size: 1476 1708 1210 3539
Fragments arranged by size:
3539 1708 1476 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7933
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3353 4010 4088
Size: 107 122 71 631 605 1681 657 78
Cuts at: 4088 4652 4763 6544 136
Size: 564 111 1781 1525
Fragments arranged by size:
1781 1681 1525 657 631 605 564 122
111 107 78 71
BglII A'GATC_T
Cuts at: 2442 7928 2442
Size: 5486 2447
BlpI GC'TnA_GC
Cuts at: 2057 2910 3656 3773 5350 2057
Size: 853 746 117 1577 4640
Fragments arranged by size:
4640 1577 853 746 117
Bme1580I G_kGCm'C
Cuts at: 1790 2195 2316 2997 3405 4006 4213 5994 7150
Size: 405 121 681 408 601 207 1781 1156
Cuts at: 7150 1790
Size: 2573
Fragments arranged by size:
2573 1781 1156 681 601 408 405 207
121
BmgBI CAC'GTC
Cuts at: 1318 2287 1318
Size: 969 6964
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2461 4045 5664
Size: 416 53 83 399 24 1044 1584 1619
Cuts at: 5664 6622 442
Size: 958 1753
Fragments arranged by size:
1753 1619 1584 1044 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7933
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3676 3858 3676
Size: 182 7751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2504 2525 3068 3152 3167 3215 3227
Size: 863 402 21 543 84 15 48 12
Cuts at: 3227 3314 3584 3605 4055 4241 4493 5006 5012
Size: 87 270 21 450 186 252 513 6
Cuts at: 5012 5024 5097 6593 1239
Size: 12 73 1496 2579
Fragments arranged by size:
2579 1496 863 543 513 450 402 270
252 186 87 84 73 48 21 21
15 12 12 6
Bpu10I CC'TnA_GC
Cuts at: 2899 3278 3626 4817 2899
Size: 379 348 1191 6015
Fragments arranged by size:
6015 1191 379 348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2441 3557 6058 6923 7221 2441
Size: 1116 2501 865 298 3153
Fragments arranged by size:
3153 2501 1116 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2364 6596 882
Size: 617 865 4232 2219
Fragments arranged by size:
4232 2219 865 617
BsaAI yAC'GTr
Cuts at: 493 1204 5050 5230 5724 493
Size: 711 3846 180 494 2702
Fragments arranged by size:
3846 2702 711 494 180
BsaBI GATnn'nnATC
Cuts at: 7927 7927
Size: 7933
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4074 5740 6122 6757
Size: 53 83 186 936 2541 1666 382 635
Cuts at: 6757 275
Size: 1451
Fragments arranged by size:
2541 1666 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2775 6366 6764 7038 7185 7596 2775
Size: 3591 398 274 147 411 3112
Fragments arranged by size:
3591 3112 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3518 3548 4046 4076 5070
Size: 30 165 30 2115 30 498 30 994
Cuts at: 5070 5100 1178
Size: 30 4011
Fragments arranged by size:
4011 2115 994 498 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2957 2975 3224 3338 3485 3560 4052 4367
Size: 1771 18 249 114 147 75 492 315
Cuts at: 4367 4739 5096 1186
Size: 372 357 4023
Fragments arranged by size:
4023 1771 492 372 357 315 249 147
114 75 18
BseYI C'CCAG_C
Cuts at: 2252 2516 3153 3273 3288 4309 4357 4662 4767
Size: 264 637 120 15 1021 48 305 105
Cuts at: 4767 5023 5057 5099 5153 5206 7136 7632 2252
Size: 256 34 42 54 53 1930 496 2553
Fragments arranged by size:
2553 1930 1021 637 496 305 264 256
120 105 54 53 48 42 34 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3944 4486 1163
Size: 573 2208 542 4610
Fragments arranged by size:
4610 2208 573 542
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4180 5383 6144 7172 1540
Size: 275 186 2179 1203 761 1028 2301
Fragments arranged by size:
2301 2179 1203 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2916 3393 3405 3662 4031 4227 4731 5994 6079
Size: 477 12 257 369 196 504 1263 85
Cuts at: 6079 7150 2916
Size: 1071 3699
Fragments arranged by size:
3699 1263 1071 504 477 369 257 196
85 12
BsmI GAATG_Cn'
Cuts at: 3193 5444 5537 3193
Size: 2251 93 5589
Fragments arranged by size:
5589 2251 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2301 4961 2301
Size: 2660 5273
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2316 2916 2997 3393 3405 3662
Size: 399 405 121 600 81 396 12 257
Cuts at: 3662 4006 4031 4213 4219 4227 4731 5252 5994
Size: 344 25 182 6 8 504 521 742
Cuts at: 5994 6079 7150 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 600 521 504 405 399
396 344 257 182 121 85 81 25
12 8 6
BspEI T'CCGG_A
Cuts at: 7596 7596
Size: 7933
BspHI T'CATG_A
Cuts at: 5822 7552 5822
Size: 1730 6203
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3505 3738 3972 4202 4963 5288 844
Size: 2661 233 234 230 761 325 3489
Fragments arranged by size:
3489 2661 761 325 234 233 230
BsrBI CCG'CTC
Cuts at: 1394 1823 3123 3304 4575 5820 1394
Size: 429 1300 181 1271 1245 3507
Fragments arranged by size:
3507 1300 1271 1245 429 181
BsrDI GCAATG_nn'
Cuts at: 65 1248 6428 6602 65
Size: 1183 5180 174 1396
Fragments arranged by size:
5180 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2775 2923 3822 6577 6764 1700
Size: 110 234 731 148 899 2755 187 2869
Fragments arranged by size:
2869 2755 899 731 234 187 148 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7933
BssHII G'CGCG_C
Cuts at: 1925 3356 3612 1925
Size: 1431 256 6246
Fragments arranged by size:
6246 1431 256
BssSI C'ACGA_G
Cuts at: 2201 2783 3263 4745 5216 5993 7005 2201
Size: 582 480 1482 471 777 1012 3129
Fragments arranged by size:
3129 1482 1012 777 582 480 471
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3856 3868 4203 4255 794
Size: 3062 12 335 52 4472
Fragments arranged by size:
4472 3062 335 52 12
BstBI TT'CG_AA
Cuts at: 2007 6737 2007
Size: 4730 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7933
BstXI CCAn_nnnn'nTGG
Cuts at: 4657 4678 4870 4657
Size: 21 192 7720
Fragments arranged by size:
7720 192 21
BstYI r'GATC_y
Cuts at: 1972 2442 2856 4488 5263 5630 6015 6032 7484
Size: 470 414 1632 775 367 385 17 1452
Cuts at: 7484 7570 7582 7928 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1632 1452 775 470 414 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2644 2801 2644
Size: 157 7776
BtgI C'CryG_G
Cuts at: 513 1065 2475 3000 3348 3915 4365 4917 513
Size: 552 1410 525 348 567 450 552 3529
Fragments arranged by size:
3529 1410 567 552 552 525 450 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2608 3148 487
Size: 683 56 1382 540 5272
Fragments arranged by size:
5272 1382 683 540 56
BtsI GCAGTG_nn'
Cuts at: 3894 5103 5449 6243 6263 3894
Size: 1209 346 794 20 5564
Fragments arranged by size:
5564 1209 794 346 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7933
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7898
DraI TTT'AAA
Cuts at: 5237 5237
Size: 7933
DraIII CAC_nnn'GTG
Cuts at: 5050 5050
Size: 7933
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6940 809
Size: 507 5624 1802
Fragments arranged by size:
5624 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2619 4386 5088
Size: 54 1425 50 275 18 789 1767 702
Cuts at: 5088 5380 6269 7898 8
Size: 292 889 1629 43
Fragments arranged by size:
1767 1629 1425 889 789 702 292 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5380 1537
Size: 275 3568 4090
Fragments arranged by size:
4090 3568 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3035 3095 3170 3545 5863 1353
Size: 1682 60 75 375 2318 3423
Fragments arranged by size:
3423 2318 1682 375 75 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3248 4069 6520 6894 7040 387
Size: 1064 138 1659 821 2451 374 146 1280
Fragments arranged by size:
2451 1659 1280 1064 821 374 146 138
EcoICRI GAG'CTC
Cuts at: 3391 3660 4225 4729 3391
Size: 269 565 504 6595
Fragments arranged by size:
6595 565 504 269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2504 2525 2603 3068 3152 3167
Size: 393 470 402 21 78 465 84 15
Cuts at: 3167 3194 3215 3227 3314 3392 3584 3605 4055
Size: 27 21 12 87 78 192 21 450
Cuts at: 4055 4241 4289 4493 4970 5006 5012 5024 5097
Size: 186 48 204 477 36 6 12 73
Cuts at: 5097 5996 6593 7380 7648 1239
Size: 899 597 787 268 1524
Fragments arranged by size:
1524 899 787 597 477 470 465 450
402 393 268 204 192 186 87 84
78 78 73 48 36 27 21 21
21 15 12 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2294 2672 3320 4037 4514 4556 2294
Size: 378 648 717 477 42 5671
Fragments arranged by size:
5671 717 648 477 378 42
EcoO109I rG'GnC_Cy
Cuts at: 2152 2299 2876 2947 2993 2994 3079 3235 3313
Size: 147 577 71 46 1 85 156 78
Cuts at: 3313 3622 4002 4209 2152
Size: 309 380 207 5876
Fragments arranged by size:
5876 577 380 309 207 156 147 85
78 71 46 1
EcoRI G'AATT_C
Cuts at: 5242 5620 5242
Size: 378 7555
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 7933
FspI TGC'GCA
Cuts at: 6439 6439
Size: 7933
HaeII r_GCGC'y
Cuts at: 1678 3773 3903 7080 1678
Size: 2095 130 3177 2531
Fragments arranged by size:
3177 2531 2095 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3824 3856 6574 6606 6648
Size: 32 135 32 2252 32 2718 32 42
Cuts at: 6648 6680 1373
Size: 32 2626
Fragments arranged by size:
2718 2626 2252 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2530 4372 5277 5523 669
Size: 988 281 592 1842 905 246 3079
Fragments arranged by size:
3079 1842 988 905 592 281 246
HindIII A'AGCT_T
Cuts at: 748 2585 748
Size: 1837 6096
HpaI GTT'AAC
Cuts at: 5523 5523
Size: 7933
KpnI G_GTAC'C
Cuts at: 2347 5258 2347
Size: 2911 5022
MfeI C'AATT_G
Cuts at: 5532 5532
Size: 7933
MluI A'CGCG_T
Cuts at: 7809 7809
Size: 7933
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2629 2850 3510 3630 7047 7231 1305
Size: 1324 221 660 120 3417 184 2007
Fragments arranged by size:
3417 2007 1324 660 221 184 120
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5090 10
Size: 54 1425 343 3258 2853
Fragments arranged by size:
3258 2853 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2319 2388 5891 6250 6409 518
Size: 648 1153 69 3503 359 159 2042
Fragments arranged by size:
3503 2042 1153 648 359 159 69
MspA1I CmG'CkG
Cuts at: 1606 2248 2271 2341 2407 2477 2516 3002 3157
Size: 642 23 70 66 70 39 486 155
Cuts at: 3157 3172 3217 3277 3292 3445 3496 3562 3718
Size: 15 45 60 15 153 51 66 156
Cuts at: 3718 3917 4237 4309 4678 4691 4771 5349 6026
Size: 199 320 72 369 13 80 578 677
Cuts at: 6026 7174 7419 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 642 578 486 369 320
245 199 156 155 153 80 72 70
70 66 66 60 51 45 39 23
15 15 13
NaeI GCC'GGC
Cuts at: 2046 3824 2046
Size: 1778 6155
NcoI C'CATG_G
Cuts at: 513 1065 4917 513
Size: 552 3852 3529
Fragments arranged by size:
3852 3529 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7933
NgoMIV G'CCGG_C
Cuts at: 2044 3822 2044
Size: 1778 6155
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7933
NotI GC'GGCC_GC
Cuts at: 5380 5380
Size: 7933
NspI r_CATG'y
Cuts at: 1132 1229 2833 4267 5020 5291 6836 1132
Size: 97 1604 1434 753 271 1545 2229
Fragments arranged by size:
2229 1604 1545 1434 753 271 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7933
PciI A'CATG_T
Cuts at: 6832 6832
Size: 7933
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2080 3268 4774 1108
Size: 972 1188 1506 4267
Fragments arranged by size:
4267 1506 1188 972
PfoI T'CCnGG_A
Cuts at: 2358 2682 4983 2358
Size: 324 2301 5308
Fragments arranged by size:
5308 2301 324
PmeI GTTT'AAAC
Cuts at: 5237 5237
Size: 7933
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6002 6034 7515 7547 6002
Size: 32 1481 32 6388
Fragments arranged by size:
6388 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2876 3079 2299
Size: 577 203 7153
Fragments arranged by size:
7153 577 203
PshAI GACnn'nnGTC
Cuts at: 1613 2525 1613
Size: 912 7021
PsiI TTA'TAA
Cuts at: 5503 5503
Size: 7933
PspOMI G'GGCC_C
Cuts at: 2993 4002 4209 2993
Size: 1009 207 6717
Fragments arranged by size:
6717 1009 207
PstI C_TGCA'G
Cuts at: 830 2693 3930 4199 4242 4296 4557 4830 5285
Size: 1863 1237 269 43 54 261 273 455
Cuts at: 5285 830
Size: 3478
Fragments arranged by size:
3478 1863 1237 455 273 269 261 54
43
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7933
PvuII CAG'CTG
Cuts at: 2271 2407 2516 3172 3277 3292 3445 3496 3562
Size: 136 109 656 105 15 153 51 66
Cuts at: 3562 4237 4309 4678 4691 4771 2271
Size: 675 72 369 13 80 5433
Fragments arranged by size:
5433 675 656 369 153 136 109 105
80 72 66 51 15 13
SacI G_AGCT'C
Cuts at: 3393 3662 4227 4731 3393
Size: 269 565 504 6595
Fragments arranged by size:
6595 565 504 269
SacII CC_GC'GG
Cuts at: 2478 3003 3918 2478
Size: 525 915 6493
Fragments arranged by size:
6493 915 525
SalI G'TCGA_C
Cuts at: 1936 4370 5275 1936
Size: 2434 905 4594
Fragments arranged by size:
4594 2434 905
SanDI GG'GwC_CC
Cuts at: 2876 2876
Size: 7933
SapI GCTCTTCn'nnn_
Cuts at: 3170 3545 3170
Size: 375 7558
SbfI CC_TGCA'GG
Cuts at: 3930 4199 4296 4830 5285 3930
Size: 269 97 534 455 6578
Fragments arranged by size:
6578 534 455 269 97
ScaI AGT'ACT
Cuts at: 6181 7893 6181
Size: 1712 6221
SexAI A'CCwGG_T
Cuts at: 3990 3990
Size: 7933
SfcI C'TryA_G
Cuts at: 826 1045 2689 3926 4195 4238 4292 4553 4826
Size: 219 1644 1237 269 43 54 261 273
Cuts at: 4826 5281 6416 7097 7288 826
Size: 455 1135 681 191 1471
Fragments arranged by size:
1644 1471 1237 1135 681 455 273 269
261 219 191 54 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 4010 4010
Size: 7933
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7933
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7933
SmaI CCC'GGG
Cuts at: 1872 4214 5260 1872
Size: 2342 1046 4545
Fragments arranged by size:
4545 2342 1046
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2456 3572 6037 6938 7200 820
Size: 197 926 513 1116 2465 901 262 1553
Fragments arranged by size:
2465 1553 1116 926 901 513 262 197
SnaBI TAC'GTA
Cuts at: 493 5230 5724 493
Size: 4737 494 2702
Fragments arranged by size:
4737 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7933
SphI G_CATG'C
Cuts at: 1132 5020 5291 1132
Size: 3888 271 3774
Fragments arranged by size:
3888 3774 271
SrfI GCCC'GGGC
Cuts at: 4214 4214
Size: 7933
SspI AAT'ATT
Cuts at: 5 52 5857 5
Size: 47 5805 2081
Fragments arranged by size:
5805 2081 47
StuI AGG'CCT
Cuts at: 1517 4013 4635 1517
Size: 2496 622 4815
Fragments arranged by size:
4815 2496 622
StyI C'CwwG_G
Cuts at: 513 1065 3591 4014 4065 4650 4785 4917 5372
Size: 552 2526 423 51 585 135 132 455
Cuts at: 5372 513
Size: 3074
Fragments arranged by size:
3074 2526 585 552 455 423 135 132
51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 2537 4643 5960 5977 6130 6315
Size: 1255 579 378 2106 1317 17 153 185
Cuts at: 6315 6760 325
Size: 445 1498
Fragments arranged by size:
2106 1498 1317 1255 579 445 378 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2835 6179 7891
Size: 274 80 33 51 1443 858 3344 1712
Cuts at: 7891 96
Size: 138
Fragments arranged by size:
3344 1712 1443 858 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2394 2426 3328 3360 5070 5102 7893 7925 2394
Size: 32 902 32 1710 32 2791 32 2402
Fragments arranged by size:
2791 2402 1710 902 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7933
XbaI T'CTAG_A
Cuts at: 5269 5269
Size: 7933
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4153 4678 4153
Size: 525 7408
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7933
XmaI C'CCGG_G
Cuts at: 1870 4212 5258 1870
Size: 2342 1046 4545
Fragments arranged by size:
4545 2342 1046
XmnI GAAnn'nnTTC
Cuts at: 2810 6062 2810
Size: 3252 4681
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5741 276
Size: 53 83 186 936 4207 2468
Fragments arranged by size:
4207 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SfoI SwaI