Restriction map for
pFN21ASDA0654
(Circular) (Six-base) MAPSORT of: pFN21ASDA0654.seq Check: 3924 from: 1 to: 8311
pFN21ASDA0654 8311 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6121 278
Size: 53 83 186 936 4585 2468
Fragments arranged by size:
4585 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5041 5654 1937
Size: 3104 613 4594
Fragments arranged by size:
4594 3104 613
Acc65I G'GTAC_C
Cuts at: 5632 5632
Size: 8311
AclI AA'CG_TT
Cuts at: 1563 6438 6811 1563
Size: 4875 373 3063
Fragments arranged by size:
4875 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2360 3422 3824 5207 6374 7758 8026 1632
Size: 728 1062 402 1383 1167 1384 268 1917
Fragments arranged by size:
1917 1384 1383 1167 1062 728 402 268
AfeI AGC'GCT
Cuts at: 2954 3353 2954
Size: 399 7912
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8114
AflIII A'CryG_T
Cuts at: 1203 4779 7210 8187 1203
Size: 3576 2431 977 1327
Fragments arranged by size:
3576 2431 1327 977
AgeI A'CCGG_T
Cuts at: 7142 7142
Size: 8311
AhdI GACnn_n'nnGTC
Cuts at: 7040 7040
Size: 8311
AleI CACnn'nnGTG
Cuts at: 1166 4550 1166
Size: 3384 4927
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2270 2700 4703 1184
Size: 1086 430 2003 4792
Fragments arranged by size:
4792 2003 1086 430
AlwNI CAG_nnn'CTG
Cuts at: 2311 3103 3115 3826 3978 4861 5320 6054 7626
Size: 792 12 711 152 883 459 734 1572
Cuts at: 7626 2311
Size: 2996
Fragments arranged by size:
2996 1572 883 792 734 711 459 152
12
ApaI G_GGCC'C
Cuts at: 2084 3271 4545 2084
Size: 1187 1274 5850
Fragments arranged by size:
5850 1274 1187
ApaLI G'TGCA_C
Cuts at: 6368 7524 6368
Size: 1156 7155
ApoI r'AATT_y
Cuts at: 1493 3202 5192 5330 5620 5846 5998 8107 1493
Size: 1709 1990 138 290 226 152 2109 1697
Fragments arranged by size:
2109 1990 1709 1697 290 226 152 138
AseI AT'TA_AT
Cuts at: 160 1032 6865 160
Size: 872 5833 1606
Fragments arranged by size:
5833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8311
AvaI C'yCGr_G
Cuts at: 1870 1943 2449 2573 3137 4829 5568 5636 1870
Size: 73 506 124 564 1692 739 68 4545
Fragments arranged by size:
4545 1692 739 564 506 124 73 68
BamHI G'GATC_C
Cuts at: 3613 5641 6008 3613
Size: 2028 367 5916
Fragments arranged by size:
5916 2028 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2834 3583 3602 4060 4085
Size: 325 217 627 1047 749 19 458 25
Cuts at: 4085 4268 4625 5632 7087 618
Size: 183 357 1007 1455 1842
Fragments arranged by size:
1842 1455 1047 1007 749 627 458 357
325 217 183 25 19
BanII G_rGCy'C
Cuts at: 1391 2072 2084 2211 3271 3570 3681 3716 4129
Size: 681 12 127 1060 299 111 35 413
Cuts at: 4129 4545 4998 5506 5513 5523 5630 1391
Size: 416 453 508 7 10 107 4072
Fragments arranged by size:
4072 1060 681 508 453 416 413 299
127 111 107 35 12 10 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7340
BbvCI CC'TCA_GC
Cuts at: 4744 4744
Size: 8311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6502 6536 1903
Size: 34 4565 34 3678
Fragments arranged by size:
4565 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6203 7413 6203
Size: 1210 7101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2067 4515 4607
Size: 107 122 71 631 605 395 2448 92
Cuts at: 4607 4634 6922 136
Size: 27 2288 1525
Fragments arranged by size:
2448 2288 1525 631 605 395 122 107
92 71 27
BglII A'GATC_T
Cuts at: 8306 8306
Size: 8311
BlpI GC'TnA_GC
Cuts at: 2243 3335 5728 2243
Size: 1092 2393 4826
Fragments arranged by size:
4826 2393 1092
Bme1580I G_kGCm'C
Cuts at: 1790 2084 2837 3271 3562 3607 4088 4271 4545
Size: 294 753 434 291 45 481 183 274
Cuts at: 4545 5233 6372 7528 1790
Size: 688 1139 1156 2573
Fragments arranged by size:
2573 1156 1139 753 688 481 434 294
291 274 183 45
BmgBI CAC'GTC
Cuts at: 1318 2982 3817 5134 1318
Size: 1664 835 1317 4495
Fragments arranged by size:
4495 1664 1317 835
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2437 3795 6042
Size: 416 53 83 399 24 1020 1358 2247
Cuts at: 6042 7000 442
Size: 958 1753
Fragments arranged by size:
2247 1753 1358 1020 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3027 4439 1056
Size: 1971 1412 4928
Fragments arranged by size:
4928 1971 1412
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 3418 3697 4744 2242
Size: 1176 279 1047 5809
Fragments arranged by size:
5809 1176 1047 279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2171 2333 2402 2576 2756 2768 2840
Size: 887 45 162 69 174 180 12 72
Cuts at: 2840 2966 3029 3248 3410 3523 3584 3872 3944
Size: 126 63 219 162 113 61 288 72
Cuts at: 3944 4143 4517 4583 5114 5342 6971 1239
Size: 199 374 66 531 228 1629 2579
Fragments arranged by size:
2579 1629 887 531 374 288 228 219
199 180 174 162 162 126 113 72
72 69 66 63 61 45 12
Bpu10I CC'TnA_GC
Cuts at: 2960 3407 4744 2960
Size: 447 1337 6527
Fragments arranged by size:
6527 1337 447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2879 3353 4214 4682 4994 6436 7301 7599 2879
Size: 474 861 468 312 1442 865 298 3591
Fragments arranged by size:
3591 1442 865 861 474 468 312 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2464 3122 3549 4732 4826 5243 6974
Size: 617 965 658 427 1183 94 417 1731
Cuts at: 6974 882
Size: 2219
Fragments arranged by size:
2219 1731 1183 965 658 617 427 417
94
BsaAI yAC'GTr
Cuts at: 493 1204 5608 6102 493
Size: 711 4404 494 2702
Fragments arranged by size:
4404 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3500 4673 8305 3500
Size: 1173 3632 3506
Fragments arranged by size:
3632 3506 1173
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2994 3090 6118 6500
Size: 53 83 186 936 1461 96 3028 382
Cuts at: 6500 7135 275
Size: 635 1451
Fragments arranged by size:
3028 1461 1451 936 635 382 186 96
83 53
BsaWI w'CCGG_w
Cuts at: 5082 5589 6744 7142 7416 7563 7974 5082
Size: 507 1155 398 274 147 411 5419
Fragments arranged by size:
5419 1155 507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2813 2843 2966 2996 3002
Size: 30 165 30 1410 30 123 30 6
Cuts at: 3002 3023 3032 3053 4790 4820 5330 5360 1178
Size: 21 9 21 1737 30 510 30 4129
Fragments arranged by size:
4129 1737 1410 510 165 123 30 30
30 30 30 30 21 21 9 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2452 2591 2594 2753 2837 2840 2942 3224
Size: 1266 139 3 159 84 3 102 282
Cuts at: 3224 3407 3428 3689 3773 3776 3902 3932 4514
Size: 183 21 261 84 3 126 30 582
Cuts at: 4514 4747 4811 1186
Size: 233 64 4686
Fragments arranged by size:
4686 1266 582 282 261 233 183 159
139 126 102 84 84 64 30 21
3 3 3
BseYI C'CCAG_C
Cuts at: 2077 2851 2967 3234 4031 4848 4910 5179 7514
Size: 774 116 267 797 817 62 269 2335
Cuts at: 7514 8010 2077
Size: 496 2378
Fragments arranged by size:
2378 2335 817 797 774 496 269 267
116 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2972 3839 4268 4673 1163
Size: 573 1236 867 429 405 4801
Fragments arranged by size:
4801 1236 867 573 429 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5761 6522 7550 1540
Size: 275 186 3760 761 1028 2301
Fragments arranged by size:
3760 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2481 2508 2574 3570 4129 4998 6372 6457
Size: 270 27 66 996 559 869 1374 85
Cuts at: 6457 7528 2211
Size: 1071 2994
Fragments arranged by size:
2994 1374 1071 996 869 559 270 85
66 27
BsmI GAATG_Cn'
Cuts at: 5305 5822 5915 5305
Size: 517 93 7701
Fragments arranged by size:
7701 517 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2147 2988 3311 4334 5124 2147
Size: 841 323 1023 790 5334
Fragments arranged by size:
5334 1023 841 790 323
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2084 2211 2481 2508 2574 2837
Size: 399 282 12 127 270 27 66 263
Cuts at: 2837 3271 3562 3570 3607 3681 3716 4088 4129
Size: 434 291 8 37 74 35 372 41
Cuts at: 4129 4271 4545 4998 5233 5506 5513 5523 5630
Size: 142 274 453 235 273 7 10 107
Cuts at: 5630 6372 6457 7528 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 434 399 372 291
282 274 273 270 263 235 142 127
107 85 74 66 41 37 35 27
12 10 8 7
BspEI T'CCGG_A
Cuts at: 5589 7974 5589
Size: 2385 5926
BspHI T'CATG_A
Cuts at: 6200 7930 6200
Size: 1730 6581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5666 844
Size: 4822 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2539 2752 2932 3214 3487 3697 4182
Size: 429 716 213 180 282 273 210 485
Cuts at: 4182 4623 5098 5155 5217 5400 6198 1394
Size: 441 475 57 62 183 798 3507
Fragments arranged by size:
3507 798 716 485 475 441 429 282
273 213 210 183 180 62 57
BsrDI GCAATG_nn'
Cuts at: 65 1248 6806 6980 65
Size: 1183 5558 174 1396
Fragments arranged by size:
5558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2061 2718 4144 6955 7142 1700
Size: 110 251 657 1426 2811 187 2869
Fragments arranged by size:
2869 2811 1426 657 251 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8311
BssHII G'CGCG_C
Cuts at: 1925 2758 1925
Size: 833 7478
BssSI C'ACGA_G
Cuts at: 2216 2402 4131 4793 4883 6371 7383 2216
Size: 186 1729 662 90 1488 1012 3144
Fragments arranged by size:
3144 1729 1488 1012 662 186 90
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3103 3115 3978 794
Size: 2309 12 863 5127
Fragments arranged by size:
5127 2309 863 12
BstBI TT'CG_AA
Cuts at: 2007 7115 2007
Size: 5108 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8311
BstXI CCAn_nnnn'nTGG
Cuts at: 2695 5558 2695
Size: 2863 5448
BstYI r'GATC_y
Cuts at: 1972 3613 4656 4857 5124 5286 5641 6008 6393
Size: 1641 1043 201 267 162 355 367 385
Cuts at: 6393 6410 7862 7948 7960 8306 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1641 1452 1043 385 367 355 346
267 201 162 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4110 5350 4110
Size: 1240 7071
BtgI C'CryG_G
Cuts at: 513 1065 2424 4253 4548 513
Size: 552 1359 1829 295 4276
Fragments arranged by size:
4276 1829 1359 552 295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2233 487
Size: 683 56 1007 6565
Fragments arranged by size:
6565 1007 683 56
BtsI GCAGTG_nn'
Cuts at: 2411 2574 3562 3637 4217 5231 5827 6621 6641
Size: 163 988 75 580 1014 596 794 20
Cuts at: 6641 2411
Size: 4081
Fragments arranged by size:
4081 1014 988 794 596 580 163 75
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8276
DraI TTT'AAA
Cuts at: 5615 5615
Size: 8311
DraIII CAC_nnn'GTG
Cuts at: 5035 5035
Size: 8311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7318 809
Size: 507 6002 1802
Fragments arranged by size:
6002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2059 2212 3072
Size: 54 1425 50 275 18 229 153 860
Cuts at: 3072 3474 3828 4039 4146 4416 4596 4635 4815
Size: 402 354 211 107 270 180 39 180
Cuts at: 4815 5758 6647 8276 8
Size: 943 889 1629 43
Fragments arranged by size:
1629 1425 943 889 860 402 354 275
270 229 211 180 180 153 107 54
50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 5758 1537
Size: 275 3946 4090
Fragments arranged by size:
4090 3946 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 2504 2646 3048 3053 3237 4298 4387
Size: 966 185 142 402 5 184 1061 89
Cuts at: 4387 6241 1353
Size: 1854 3423
Fragments arranged by size:
3423 1854 1061 966 402 185 184 142
89 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2360 5428 6898 7272 7418 387
Size: 1064 138 771 3068 1470 374 146 1280
Fragments arranged by size:
3068 1470 1280 1064 771 374 146 138
EcoICRI GAG'CTC
Cuts at: 2209 3568 4127 4996 2209
Size: 1359 559 869 5524
Fragments arranged by size:
5524 1359 869 559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2171 2333 2360 2402 2576 2756
Size: 393 494 45 162 27 42 174 180
Cuts at: 2756 2768 2840 2966 3029 3248 3410 3422 3523
Size: 12 72 126 63 219 162 12 101
Cuts at: 3523 3584 3824 3872 3944 4143 4517 4583 5114
Size: 61 240 48 72 199 374 66 531
Cuts at: 5114 5207 5342 6374 6971 7758 8026 1239
Size: 93 135 1032 597 787 268 1524
Fragments arranged by size:
1524 1032 787 597 531 494 393 374
268 240 219 199 180 174 162 162
135 126 101 93 72 72 66 63
61 48 45 42 27 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2897 3410 3538 4499 2897
Size: 513 128 961 6709
Fragments arranged by size:
6709 961 513 128
EcoO109I rG'GnC_Cy
Cuts at: 2515 2699 2743 4230 4247 4361 4516 5089 5434
Size: 184 44 1487 17 114 155 573 345
Cuts at: 5434 2515
Size: 5392
Fragments arranged by size:
5392 1487 573 345 184 155 114 44
17
EcoRI G'AATT_C
Cuts at: 5330 5620 5998 5330
Size: 290 378 7643
Fragments arranged by size:
7643 378 290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 8311
FseI GG_CCGG'CC
Cuts at: 4148 4148
Size: 8311
FspI TGC'GCA
Cuts at: 2417 6817 2417
Size: 4400 3911
HaeII r_GCGC'y
Cuts at: 1678 2078 2113 2146 2239 2437 2555 2788 2906
Size: 400 35 33 93 198 118 233 118
Cuts at: 2906 2956 2967 3176 3355 3389 5385 7458 1678
Size: 50 11 209 179 34 1996 2073 2531
Fragments arranged by size:
2531 2073 1996 400 233 209 198 179
118 118 93 50 35 34 33 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2966 2998 3779 3811 4790
Size: 32 135 32 1394 32 781 32 979
Cuts at: 4790 4822 6952 6984 7026 7058 1373
Size: 32 2130 32 42 32 2626
Fragments arranged by size:
2626 2130 1394 979 781 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5655 5901 669
Size: 988 281 3717 246 3079
Fragments arranged by size:
3717 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3434 748
Size: 2686 5625
HpaI GTT'AAC
Cuts at: 5901 5901
Size: 8311
KpnI G_GTAC'C
Cuts at: 5636 5636
Size: 8311
MfeI C'AATT_G
Cuts at: 5910 5910
Size: 8311
MluI A'CGCG_T
Cuts at: 8187 8187
Size: 8311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2739 3063 3369 5289 7425 7609 1305
Size: 1434 324 306 1920 2136 184 2007
Fragments arranged by size:
2136 2007 1920 1434 324 306 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3830 4041 4418 4637 10
Size: 54 1425 343 1998 211 377 219 3684
Fragments arranged by size:
3684 1998 1425 377 343 219 211 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 4550 6269 6628 6787 518
Size: 648 1257 2127 1719 359 159 2042
Fragments arranged by size:
2127 2042 1719 1257 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2194 2308 2626 2776 2818 2914 2971 3070
Size: 588 114 318 150 42 96 57 99
Cuts at: 3070 3154 3226 3238 3265 3277 3511 3657 3850
Size: 84 72 12 27 12 234 146 193
Cuts at: 3850 3978 4687 4848 5462 5498 5727 6404 7552
Size: 128 709 161 614 36 229 677 1148
Cuts at: 7552 7797 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 709 677 614 588 318 245
234 229 193 161 150 146 128 114
99 96 84 72 57 42 36 27
12 12
NaeI GCC'GGC
Cuts at: 2063 4146 2063
Size: 2083 6228
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7759
NdeI CA'TA_TG
Cuts at: 387 4867 387
Size: 4480 3831
NgoMIV G'CCGG_C
Cuts at: 2061 4144 2061
Size: 2083 6228
NheI G'CTAG_C
Cuts at: 1052 3023 4435 1052
Size: 1971 1412 4928
Fragments arranged by size:
4928 1971 1412
NotI GC'GGCC_GC
Cuts at: 5758 5758
Size: 8311
NspI r_CATG'y
Cuts at: 1132 1229 4582 5669 7214 1132
Size: 97 3353 1087 1545 2229
Fragments arranged by size:
3353 2229 1545 1087 97
PasI CC'CwG_GG
Cuts at: 1348 3734 5506 1348
Size: 2386 1772 4153
Fragments arranged by size:
4153 2386 1772
PciI A'CATG_T
Cuts at: 7210 7210
Size: 8311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3690 3734 4213 4723 1108
Size: 2582 44 479 510 4696
Fragments arranged by size:
4696 2582 510 479 44
PfoI T'CCnGG_A
Cuts at: 2370 2532 4494 4716 5424 2370
Size: 162 1962 222 708 5257
Fragments arranged by size:
5257 1962 708 222 162
PmeI GTTT'AAAC
Cuts at: 5615 5615
Size: 8311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6380 6412 7893 7925 6380
Size: 32 1481 32 6766
Fragments arranged by size:
6766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2699 4230 4247 4361 5089 5434 2699
Size: 1531 17 114 728 345 5576
Fragments arranged by size:
5576 1531 728 345 114 17
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8311
PsiI TTA'TAA
Cuts at: 5881 5881
Size: 8311
PspOMI G'GGCC_C
Cuts at: 2080 3267 4541 2080
Size: 1187 1274 5850
Fragments arranged by size:
5850 1274 1187
PspXI vC'TCGA_Gb
Cuts at: 2573 2573
Size: 8311
PstI C_TGCA'G
Cuts at: 830 2235 2886 3108 3120 3264 4224 5286 5497
Size: 1405 651 222 12 144 960 1062 211
Cuts at: 5497 5541 5663 830
Size: 44 122 3478
Fragments arranged by size:
3478 1405 1062 960 651 222 211 144
122 44 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8311
PvuII CAG'CTG
Cuts at: 2194 2308 2626 2776 2818 3070 3154 3226 3238
Size: 114 318 150 42 252 84 72 12
Cuts at: 3238 3511 3850 3978 4848 5462 2194
Size: 273 339 128 870 614 5043
Fragments arranged by size:
5043 870 614 339 318 273 252 150
128 114 84 72 42 12
RsrII CG'GwC_CG
Cuts at: 5587 5587
Size: 8311
SacI G_AGCT'C
Cuts at: 2211 3570 4129 4998 2211
Size: 1359 559 869 5524
Fragments arranged by size:
5524 1359 869 559
SalI G'TCGA_C
Cuts at: 1936 5653 1936
Size: 3717 4594
SanDI GG'GwC_CC
Cuts at: 4230 4247 4361 4230
Size: 17 114 8180
Fragments arranged by size:
8180 114 17
SapI GCTCTTCn'nnn_
Cuts at: 2646 3053 4298 4387 2646
Size: 407 1245 89 6570
Fragments arranged by size:
6570 1245 407 89
SbfI CC_TGCA'GG
Cuts at: 5663 5663
Size: 8311
ScaI AGT'ACT
Cuts at: 6559 8271 6559
Size: 1712 6599
SfcI C'TryA_G
Cuts at: 826 1045 2184 2231 2882 3104 3116 3260 4220
Size: 219 1139 47 651 222 12 144 960
Cuts at: 4220 5282 5493 5537 5659 6794 7475 7666 826
Size: 1062 211 44 122 1135 681 191 1471
Fragments arranged by size:
1471 1139 1135 1062 960 681 651 222
219 211 191 144 122 47 44 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8311
SmaI CCC'GGG
Cuts at: 1872 2451 5570 5638 1872
Size: 579 3119 68 4545
Fragments arranged by size:
4545 3119 579 68
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2573 2894 3368 4193 4697 5009
Size: 197 926 630 321 474 825 504 312
Cuts at: 5009 6415 7316 7578 820
Size: 1406 901 262 1553
Fragments arranged by size:
1553 1406 926 901 825 630 504 474
321 312 262 197
SnaBI TAC'GTA
Cuts at: 493 5608 6102 493
Size: 5115 494 2702
Fragments arranged by size:
5115 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8311
SphI G_CATG'C
Cuts at: 1132 4582 5669 1132
Size: 3450 1087 3774
Fragments arranged by size:
3774 3450 1087
SspI AAT'ATT
Cuts at: 5 52 6235 5
Size: 47 6183 2081
Fragments arranged by size:
6183 2081 47
StuI AGG'CCT
Cuts at: 1517 4211 4505 1517
Size: 2694 294 5323
Fragments arranged by size:
5323 2694 294
StyI C'CwwG_G
Cuts at: 513 1065 3684 4212 4836 5750 513
Size: 552 2619 528 624 914 3074
Fragments arranged by size:
3074 2619 914 624 552 528
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3601 4186 4482 4561 4879 5158 5575
Size: 1255 2021 585 296 79 318 279 417
Cuts at: 5575 6338 6355 6508 6693 7138 325
Size: 763 17 153 185 445 1498
Fragments arranged by size:
2021 1498 1255 763 585 445 417 318
296 279 185 153 79 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6557 8269 96
Size: 274 80 33 51 1443 4580 1712 138
Fragments arranged by size:
4580 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3714 3746 8271 8303 3714
Size: 32 4525 32 3722
Fragments arranged by size:
4525 3722 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8311
XbaI T'CTAG_A
Cuts at: 5647 5647
Size: 8311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2450 3004 3946 4426 4720 2450
Size: 554 942 480 294 6041
Fragments arranged by size:
6041 942 554 480 294
XhoI C'TCGA_G
Cuts at: 1943 2573 1943
Size: 630 7681
XmaI C'CCGG_G
Cuts at: 1870 2449 5568 5636 1870
Size: 579 3119 68 4545
Fragments arranged by size:
4545 3119 579 68
XmnI GAAnn'nnTTC
Cuts at: 5330 5420 6440 5330
Size: 90 1020 7201
Fragments arranged by size:
7201 1020 90
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6119 276
Size: 53 83 186 936 4585 2468
Fragments arranged by size:
4585 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoRV FspAI KasI NarI NruI NsiI PacI
PmlI PsrI SacII SexAI SfiI SfoI SrfI SwaI