Restriction map for
pFN21ASDA0652
(Circular) (Six-base) MAPSORT of: pFN21ASDA0652.seq Check: 957 from: 1 to: 6280
pFN21ASDA0652 6280 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4090 278
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2612 3623 1937
Size: 675 1011 4594
Fragments arranged by size:
4594 1011 675
Acc65I G'GTAC_C
Cuts at: 2950 2981 3601 2950
Size: 31 620 5629
Fragments arranged by size:
5629 620 31
AclI AA'CG_TT
Cuts at: 1563 4407 4780 1563
Size: 2844 373 3063
Fragments arranged by size:
3063 2844 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2417 3510 4343 5727 5995 1632
Size: 785 1093 833 1384 268 1917
Fragments arranged by size:
1917 1384 1093 833 785 268
AflII C'TTAA_G
Cuts at: 820 1017 2279 820
Size: 197 1262 4821
Fragments arranged by size:
4821 1262 197
AflIII A'CryG_T
Cuts at: 1203 5179 6156 1203
Size: 3976 977 1327
Fragments arranged by size:
3976 1327 977
AgeI A'CCGG_T
Cuts at: 5111 5111
Size: 6280
AhdI GACnn_n'nnGTC
Cuts at: 5009 5009
Size: 6280
AleI CACnn'nnGTG
Cuts at: 1166 2558 1166
Size: 1392 4888
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6280
AlwNI CAG_nnn'CTG
Cuts at: 3278 4023 5595 3278
Size: 745 1572 3963
Fragments arranged by size:
3963 1572 745
ApaLI G'TGCA_C
Cuts at: 4337 5493 4337
Size: 1156 5124
ApoI r'AATT_y
Cuts at: 1493 2075 3223 3589 3815 3967 6076 1493
Size: 582 1148 366 226 152 2109 1697
Fragments arranged by size:
2109 1697 1148 582 366 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4834 160
Size: 872 3802 1606
Fragments arranged by size:
3802 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
AvaI C'yCGr_G
Cuts at: 1870 1943 3605 1870
Size: 73 1662 4545
Fragments arranged by size:
4545 1662 73
BamHI G'GATC_C
Cuts at: 3610 3977 3610
Size: 367 5913
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2552 2564 2950 2981 3601
Size: 325 217 627 765 12 386 31 620
Cuts at: 3601 5056 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 765 627 620 386 325 217
31 12
BanII G_rGCy'C
Cuts at: 1391 3599 1391
Size: 2208 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2766 3498 928
Size: 971 867 732 3710
Fragments arranged by size:
3710 971 867 732
BbvCI CC'TCA_GC
Cuts at: 3444 3444
Size: 6280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3533 3567 4471 4505 1903
Size: 34 1596 34 904 34 3678
Fragments arranged by size:
3678 1596 904 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3058 3232 4172 5382 3058
Size: 174 940 1210 3956
Fragments arranged by size:
3956 1210 940 174
BclI T'GATC_A
Cuts at: 1548 2659 1548
Size: 1111 5169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4891 136
Size: 107 122 71 631 605 3219 1525
Fragments arranged by size:
3219 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6275 6275
Size: 6280
BlpI GC'TnA_GC
Cuts at: 3449 3697 3449
Size: 248 6032
Bme1580I G_kGCm'C
Cuts at: 1790 2555 2567 4341 5497 1790
Size: 765 12 1774 1156 2573
Fragments arranged by size:
2573 1774 1156 765 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6280
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4011 4969 442
Size: 416 53 83 399 24 2594 958 1753
Fragments arranged by size:
2594 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2428 2757 3037 3246 3254 3275 3301 4940
Size: 1189 329 280 209 8 21 26 1639
Cuts at: 4940 1239
Size: 2579
Fragments arranged by size:
2579 1639 1189 329 280 209 26 21
8
Bpu10I CC'TnA_GC
Cuts at: 2512 3444 2512
Size: 932 5348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2072 2751 2800 4405 5270 5568 2072
Size: 679 49 1605 865 298 2784
Fragments arranged by size:
2784 1605 865 679 298 49
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3116 4943 882
Size: 617 1617 1827 2219
Fragments arranged by size:
2219 1827 1617 617
BsaAI yAC'GTr
Cuts at: 493 1204 3577 4071 493
Size: 711 2373 494 2702
Fragments arranged by size:
2702 2373 711 494
BsaBI GATnn'nnATC
Cuts at: 3243 6274 3243
Size: 3031 3249
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4087 4469 5104 275
Size: 53 83 186 936 2554 382 635 1451
Fragments arranged by size:
2554 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4713 5111 5385 5532 5943 4713
Size: 398 274 147 411 5050
Fragments arranged by size:
5050 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3268 3298 1178
Size: 30 165 30 1865 30 4160
Fragments arranged by size:
4160 1865 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2680 3276 1186
Size: 1494 596 4190
Fragments arranged by size:
4190 1494 596
BseYI C'CCAG_C
Cuts at: 2684 2808 5483 5979 2684
Size: 124 2675 496 2985
Fragments arranged by size:
2985 2675 496 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2998 3287 1163
Size: 573 1262 289 4156
Fragments arranged by size:
4156 1262 573 289
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3730 4491 5519 1540
Size: 275 186 1729 761 1028 2301
Fragments arranged by size:
2301 1729 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3478 4341 4426 5497 3478
Size: 863 85 1071 4261
Fragments arranged by size:
4261 1071 863 85
BsmI GAATG_Cn'
Cuts at: 2603 3791 3884 2603
Size: 1188 93 4999
Fragments arranged by size:
4999 1188 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2555 2567 3478 3599 4341 4426 5497
Size: 399 765 12 911 121 742 85 1071
Cuts at: 5497 1391
Size: 2174
Fragments arranged by size:
2174 1071 911 765 742 399 121 85
12
BspEI T'CCGG_A
Cuts at: 5943 5943
Size: 6280
BspHI T'CATG_A
Cuts at: 4169 5899 4169
Size: 1730 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3020 3635 844
Size: 2176 615 3489
Fragments arranged by size:
3489 2176 615
BsrBI CCG'CTC
Cuts at: 1394 1823 4167 1394
Size: 429 2344 3507
Fragments arranged by size:
3507 2344 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2387 4775 4949 65
Size: 1183 1139 2388 174 1396
Fragments arranged by size:
2388 1396 1183 1139 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4924 5111 1700
Size: 110 3114 187 2869
Fragments arranged by size:
3114 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2373 96
Size: 2277 4003
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6280
BssSI C'ACGA_G
Cuts at: 4340 5352 4340
Size: 1012 5268
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6280
BstBI TT'CG_AA
Cuts at: 2007 5084 2007
Size: 3077 3203
BstEII G'GTnAC_C
Cuts at: 1184 3175 1184
Size: 1991 4289
BstXI CCAn_nnnn'nTGG
Cuts at: 2266 2308 3226 2266
Size: 42 918 5320
Fragments arranged by size:
5320 918 42
BstYI r'GATC_y
Cuts at: 1972 3610 3977 4362 4379 5831 5917 5929 6275
Size: 1638 367 385 17 1452 86 12 346
Cuts at: 6275 1972
Size: 1977
Fragments arranged by size:
1977 1638 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2301 2977 3483 513
Size: 552 957 279 676 506 3310
Fragments arranged by size:
3310 957 676 552 506 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5541
Fragments arranged by size:
5541 683 56
BtsI GCAGTG_nn'
Cuts at: 3094 3337 3796 4590 4610 3094
Size: 243 459 794 20 4764
Fragments arranged by size:
4764 794 459 243 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6280
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6245
DraI TTT'AAA
Cuts at: 3370 3584 3370
Size: 214 6066
DraIII CAC_nnn'GTG
Cuts at: 2668 2668
Size: 6280
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5287 809
Size: 507 3971 1802
Fragments arranged by size:
3971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3727 4616 6245
Size: 54 1425 50 275 18 1897 889 1629
Cuts at: 6245 8
Size: 43
Fragments arranged by size:
1897 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3727 1537
Size: 275 1915 4090
Fragments arranged by size:
4090 1915 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4210 1353
Size: 2857 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4867 5241 5387 387
Size: 1064 138 3278 374 146 1280
Fragments arranged by size:
3278 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2428 2757 3037 3246 3254 3275
Size: 393 785 11 329 280 209 8 21
Cuts at: 3275 3301 3510 4343 4940 5727 5995 1239
Size: 26 209 833 597 787 268 1524
Fragments arranged by size:
1524 833 787 785 597 393 329 280
268 209 209 26 21 11 8
EcoO109I rG'GnC_Cy
Cuts at: 2056 2631 3414 2056
Size: 575 783 4922
Fragments arranged by size:
4922 783 575
EcoRI G'AATT_C
Cuts at: 3589 3967 3589
Size: 378 5902
FspI TGC'GCA
Cuts at: 4786 4786
Size: 6280
HaeII r_GCGC'y
Cuts at: 1678 5427 1678
Size: 3749 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2122 2154 3266 3268 3298
Size: 32 135 32 550 32 1112 2 30
Cuts at: 3298 3300 3406 3438 3485 3517 4921 4953 4995
Size: 2 106 32 47 32 1404 32 42
Cuts at: 4995 5027 1373
Size: 32 2626
Fragments arranged by size:
2626 1404 1112 550 135 106 47 42
32 32 32 32 32 32 32 30
2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3157 3624 3870 669
Size: 988 281 1219 467 246 3079
Fragments arranged by size:
3079 1219 988 467 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6280
HpaI GTT'AAC
Cuts at: 3870 3870
Size: 6280
KpnI G_GTAC'C
Cuts at: 2954 2985 3605 2954
Size: 31 620 5629
Fragments arranged by size:
5629 620 31
MfeI C'AATT_G
Cuts at: 3879 3879
Size: 6280
MluI A'CGCG_T
Cuts at: 6156 6156
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2180 2836 5394 5578 1305
Size: 875 656 2558 184 2007
Fragments arranged by size:
2558 2007 875 656 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4458
Fragments arranged by size:
4458 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 2463 2558 3309 4238 4597 4756
Size: 648 1098 199 95 751 929 359 159
Cuts at: 4756 518
Size: 2042
Fragments arranged by size:
2042 1098 929 751 648 359 199 159
95
MspA1I CmG'CkG
Cuts at: 1606 2239 2503 2864 2923 3321 3448 3696 4373
Size: 633 264 361 59 398 127 248 677
Cuts at: 4373 5521 5766 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 633 398 361 264 248
245 127 59
NcoI C'CATG_G
Cuts at: 513 1065 2022 2301 2977 3483 513
Size: 552 957 279 676 506 3310
Fragments arranged by size:
3310 957 676 552 506 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6280
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6280
NotI GC'GGCC_GC
Cuts at: 3727 3727
Size: 6280
NspI r_CATG'y
Cuts at: 1132 1229 3638 5183 1132
Size: 97 2409 1545 2229
Fragments arranged by size:
2409 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2848 1348
Size: 1500 4780
PciI A'CATG_T
Cuts at: 5179 5179
Size: 6280
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2926 1108
Size: 1818 4462
PfoI T'CCnGG_A
Cuts at: 2444 2444
Size: 6280
PmeI GTTT'AAAC
Cuts at: 3584 3584
Size: 6280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4349 4381 5862 5894 4349
Size: 32 1481 32 4735
Fragments arranged by size:
4735 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2631 3414 2056
Size: 575 783 4922
Fragments arranged by size:
4922 783 575
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6280
PsiI TTA'TAA
Cuts at: 3850 3850
Size: 6280
PstI C_TGCA'G
Cuts at: 830 2248 3573 3632 830
Size: 1418 1325 59 3478
Fragments arranged by size:
3478 1418 1325 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6280
PvuII CAG'CTG
Cuts at: 2239 2503 2864 2923 3448 2239
Size: 264 361 59 525 5071
Fragments arranged by size:
5071 525 361 264 59
SalI G'TCGA_C
Cuts at: 1936 3622 1936
Size: 1686 4594
SbfI CC_TGCA'GG
Cuts at: 3632 3632
Size: 6280
ScaI AGT'ACT
Cuts at: 4528 6240 4528
Size: 1712 4568
SexAI A'CCwGG_T
Cuts at: 3171 3171
Size: 6280
SfcI C'TryA_G
Cuts at: 826 1045 2244 2434 2754 3569 3628 4763 5444
Size: 219 1199 190 320 815 59 1135 681
Cuts at: 5444 5635 826
Size: 191 1471
Fragments arranged by size:
1471 1199 1135 815 681 320 219 191
190 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6280
SmaI CCC'GGG
Cuts at: 1872 3607 1872
Size: 1735 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2087 2279 2766 2815 4384 5285
Size: 197 926 144 192 487 49 1569 901
Cuts at: 5285 5547 820
Size: 262 1553
Fragments arranged by size:
1569 1553 926 901 487 262 197 192
144 49
SnaBI TAC'GTA
Cuts at: 493 3577 4071 493
Size: 3084 494 2702
Fragments arranged by size:
3084 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6280
SphI G_CATG'C
Cuts at: 1132 3638 1132
Size: 2506 3774
SspI AAT'ATT
Cuts at: 5 52 4204 5
Size: 47 4152 2081
Fragments arranged by size:
4152 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6280
StyI C'CwwG_G
Cuts at: 513 1065 2022 2301 2977 3483 3719 513
Size: 552 957 279 676 506 236 3074
Fragments arranged by size:
3074 957 676 552 506 279 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4307 4324 4477 4662 5107 325
Size: 1255 2727 17 153 185 445 1498
Fragments arranged by size:
2727 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2373 4526 6238
Size: 274 80 33 51 1443 396 2153 1712
Cuts at: 6238 96
Size: 138
Fragments arranged by size:
2153 1712 1443 396 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2303 2335 2929 2961 6240 6272 2303
Size: 32 594 32 3279 32 2311
Fragments arranged by size:
3279 2311 594 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6280
XbaI T'CTAG_A
Cuts at: 3616 3616
Size: 6280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2117 2975 2117
Size: 858 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6280
XmaI C'CCGG_G
Cuts at: 1870 3605 1870
Size: 1735 4545
XmnI GAAnn'nnTTC
Cuts at: 4409 4409
Size: 6280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4088 276
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI