Restriction map for
pFN21ASDA0647
(Circular) (Six-base) MAPSORT of: pFN21ASDA0647.seq Check: 1316 from: 1 to: 8314
pFN21ASDA0647 8314 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6124 278
Size: 53 83 186 936 4588 2468
Fragments arranged by size:
4588 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2494 3210 5143 5657 1937
Size: 557 716 1933 514 4594
Fragments arranged by size:
4594 1933 716 557 514
Acc65I G'GTAC_C
Cuts at: 5635 5635
Size: 8314
AclI AA'CG_TT
Cuts at: 1563 6441 6814 1563
Size: 4878 373 3063
Fragments arranged by size:
4878 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2415 5144 5492 5600 6377 7761 8029
Size: 464 319 2729 348 108 777 1384 268
Cuts at: 8029 1632
Size: 1917
Fragments arranged by size:
2729 1917 1384 777 464 348 319 268
108
AflII C'TTAA_G
Cuts at: 820 1017 3464 820
Size: 197 2447 5670
Fragments arranged by size:
5670 2447 197
AflIII A'CryG_T
Cuts at: 1203 5234 7213 8190 1203
Size: 4031 1979 977 1327
Fragments arranged by size:
4031 1979 1327 977
AgeI A'CCGG_T
Cuts at: 7145 7145
Size: 8314
AhdI GACnn_n'nnGTC
Cuts at: 7043 7043
Size: 8314
AleI CACnn'nnGTG
Cuts at: 1166 5078 5187 1166
Size: 3912 109 4293
Fragments arranged by size:
4293 3912 109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5395 1184
Size: 4211 4103
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2630 2662 2630
Size: 32 8282
AlwNI CAG_nnn'CTG
Cuts at: 2599 4301 4541 6057 7629 2599
Size: 1702 240 1516 1572 3284
Fragments arranged by size:
3284 1702 1572 1516 240
ApaI G_GGCC'C
Cuts at: 2040 3709 4863 2040
Size: 1669 1154 5491
Fragments arranged by size:
5491 1669 1154
ApaLI G'TGCA_C
Cuts at: 4308 6371 7527 4308
Size: 2063 1156 5095
Fragments arranged by size:
5095 2063 1156
ApoI r'AATT_y
Cuts at: 1493 2210 3488 5399 5623 5849 6001 8110 1493
Size: 717 1278 1911 224 226 152 2109 1697
Fragments arranged by size:
2109 1911 1697 1278 717 226 224 152
AseI AT'TA_AT
Cuts at: 160 1032 6868 160
Size: 872 5836 1606
Fragments arranged by size:
5836 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8314
AvaI C'yCGr_G
Cuts at: 1870 1943 2809 4152 5639 1870
Size: 73 866 1343 1487 4545
Fragments arranged by size:
4545 1487 1343 866 73
BamHI G'GATC_C
Cuts at: 3115 5644 6011 3115
Size: 2529 367 5418
Fragments arranged by size:
5418 2529 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2316 2427 3545 5635 7090
Size: 325 217 627 529 111 1118 2090 1455
Cuts at: 7090 618
Size: 1842
Fragments arranged by size:
2090 1842 1455 1118 627 529 325 217
111
BanII G_rGCy'C
Cuts at: 1391 2040 2834 3709 3960 4863 4885 5208 5633
Size: 649 794 875 251 903 22 323 425
Cuts at: 5633 1391
Size: 4072
Fragments arranged by size:
4072 903 875 794 649 425 323 251
22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2403 4037 4356 4611 5298 5425 928
Size: 971 504 1634 319 255 687 127 3817
Fragments arranged by size:
3817 1634 971 687 504 319 255 127
BbvCI CC'TCA_GC
Cuts at: 2837 4020 4454 2837
Size: 1183 434 6697
Fragments arranged by size:
6697 1183 434
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5123 5157 6505 6539 1903
Size: 34 3186 34 1348 34 3678
Fragments arranged by size:
3678 3186 1348 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6206 7416 6206
Size: 1210 7104
BclI T'GATC_A
Cuts at: 1548 4444 5281 5488 1548
Size: 2896 837 207 4374
Fragments arranged by size:
4374 2896 837 207
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2984 3543 6925
Size: 107 122 71 631 605 1312 559 3382
Cuts at: 6925 136
Size: 1525
Fragments arranged by size:
3382 1525 1312 631 605 559 122 107
71
BglII A'GATC_T
Cuts at: 8309 8309
Size: 8314
BlpI GC'TnA_GC
Cuts at: 2363 5731 2363
Size: 3368 4946
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2582 2803 3366 3709 4312 4428 4863
Size: 250 542 221 563 343 603 116 435
Cuts at: 4863 5194 6375 7531 1790
Size: 331 1181 1156 2573
Fragments arranged by size:
2573 1181 1156 603 563 542 435 343
331 250 221 116
BmgBI CAC'GTC
Cuts at: 1318 2530 2781 1318
Size: 1212 251 6851
Fragments arranged by size:
6851 1212 251
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2577 2835 3785
Size: 416 53 83 399 24 1160 258 950
Cuts at: 3785 4750 5501 6045 7003 442
Size: 965 751 544 958 1753
Fragments arranged by size:
1753 1160 965 958 950 751 544 416
399 258 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8314
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 8314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2563 2654 2928 3161 3419 3974 4203
Size: 830 494 91 274 233 258 555 229
Cuts at: 4203 5013 5181 6974 1239
Size: 810 168 1793 2579
Fragments arranged by size:
2579 1793 830 810 555 494 274 258
233 229 168 91
Bpu10I CC'TnA_GC
Cuts at: 2837 4020 4454 2837
Size: 1183 434 6697
Fragments arranged by size:
6697 1183 434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4102 6439 7304 7602 4102
Size: 2337 865 298 4814
Fragments arranged by size:
4814 2337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6977 882
Size: 617 5478 2219
Fragments arranged by size:
5478 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5611 6105 493
Size: 711 4407 494 2702
Fragments arranged by size:
4407 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8308 8308
Size: 8314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6121 6503 7138 275
Size: 53 83 186 936 4588 382 635 1451
Fragments arranged by size:
4588 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2241 3069 4686 6747 7145 7419 7566 7977 2241
Size: 828 1617 2061 398 274 147 411 2578
Fragments arranged by size:
2578 2061 1617 828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2911 2941 3515 3545 1178
Size: 30 165 30 1508 30 574 30 5947
Fragments arranged by size:
5947 1508 574 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2459 2919 4041 4044 4485 5028 1186
Size: 859 414 460 1122 3 441 543 4472
Fragments arranged by size:
4472 1122 859 543 460 441 414 3
BseYI C'CCAG_C
Cuts at: 2042 3252 3665 4061 4243 4401 5313 5352 7517
Size: 1210 413 396 182 158 912 39 2165
Cuts at: 7517 8013 2042
Size: 496 2343
Fragments arranged by size:
2343 2165 1210 912 496 413 396 182
158 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 3695 1163
Size: 573 816 1143 5782
Fragments arranged by size:
5782 1143 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5764 6525 7553 1540
Size: 275 186 3763 761 1028 2301
Fragments arranged by size:
3763 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2484 2906 2921 4312 6375 6460 7531 2484
Size: 422 15 1391 2063 85 1071 3267
Fragments arranged by size:
3267 2063 1391 1071 422 85 15
BsmI GAATG_Cn'
Cuts at: 3500 5825 5918 3500
Size: 2325 93 5896
Fragments arranged by size:
5896 2325 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2484 2582 2803 2834 2906 2921
Size: 399 250 444 98 221 31 72 15
Cuts at: 2921 3366 3709 3960 4312 4428 4863 4885 5194
Size: 445 343 251 352 116 435 22 309
Cuts at: 5194 5208 5633 6375 6460 7531 1391
Size: 14 425 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 445 444 435 425 399
352 343 309 251 250 221 116 98
85 72 31 22 15 14
BspEI T'CCGG_A
Cuts at: 2241 3069 4686 7977 2241
Size: 828 1617 3291 2578
Fragments arranged by size:
3291 2578 1617 828
BspHI T'CATG_A
Cuts at: 5565 6203 7933 5565
Size: 638 1730 5946
Fragments arranged by size:
5946 1730 638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2392 3611 5669 844
Size: 1548 1219 2058 3489
Fragments arranged by size:
3489 2058 1548 1219
BsrBI CCG'CTC
Cuts at: 1394 1823 2655 3822 6201 1394
Size: 429 832 1167 2379 3507
Fragments arranged by size:
3507 2379 1167 832 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2178 2704 2761 2784 5425 5589 6809
Size: 1183 930 526 57 23 2641 164 1220
Cuts at: 6809 6983 65
Size: 174 1396
Fragments arranged by size:
2641 1396 1220 1183 930 526 174 164
57 23
BsrFI r'CCGG_y
Cuts at: 1700 1810 2196 3222 6958 7145 1700
Size: 110 386 1026 3736 187 2869
Fragments arranged by size:
3736 2869 1026 386 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8314
BssSI C'ACGA_G
Cuts at: 5550 6374 7386 5550
Size: 824 1012 6478
Fragments arranged by size:
6478 1012 824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2171 2384 4464 794
Size: 1377 213 2080 4644
Fragments arranged by size:
4644 2080 1377 213
BstBI TT'CG_AA
Cuts at: 2007 7118 2007
Size: 5111 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8314
BstXI CCAn_nnnn'nTGG
Cuts at: 3853 3853
Size: 8314
BstYI r'GATC_y
Cuts at: 1972 2068 3115 3775 3848 4528 4585 5644 6011
Size: 96 1047 660 73 680 57 1059 367
Cuts at: 6011 6396 6413 7865 7951 7963 8309 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1059 1047 680 660 385 367
346 96 86 73 57 17 12
Bsu36I CC'TnA_GG
Cuts at: 3975 4534 3975
Size: 559 7755
BtgI C'CryG_G
Cuts at: 513 1065 2022 2673 3852 4594 5073 5211 513
Size: 552 957 651 1179 742 479 138 3616
Fragments arranged by size:
3616 1179 957 742 651 552 479 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2695 487
Size: 683 56 1469 6106
Fragments arranged by size:
6106 1469 683 56
BtsI GCAGTG_nn'
Cuts at: 2345 2979 3238 4152 5182 5263 5377 5830 6624
Size: 634 259 914 1030 81 114 453 794
Cuts at: 6624 6644 2345
Size: 20 4015
Fragments arranged by size:
4015 1030 914 794 634 453 259 114
81 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3978 4013 529
Size: 35 3414 35 4830
Fragments arranged by size:
4830 3414 35 35
DraI TTT'AAA
Cuts at: 5618 5618
Size: 8314
DraIII CAC_nnn'GTG
Cuts at: 4478 4478
Size: 8314
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7321 809
Size: 507 6005 1802
Fragments arranged by size:
6005 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2976 3051 3282
Size: 54 1425 50 275 18 1146 75 231
Cuts at: 3282 3385 3555 3817 5409 5761 6650 8279 8
Size: 103 170 262 1592 352 889 1629 43
Fragments arranged by size:
1629 1592 1425 1146 889 352 275 262
231 170 103 75 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5761 1537
Size: 275 3949 4090
Fragments arranged by size:
4090 3949 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2097 3006 4157 4194 6244 1353
Size: 744 909 1151 37 2050 3423
Fragments arranged by size:
3423 2050 1151 909 744 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2519 6901 7275 7421 387
Size: 1064 138 930 4382 374 146 1280
Fragments arranged by size:
4382 1280 1064 930 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2096 2415 2563 2654 2928 3161
Size: 393 437 27 319 148 91 274 233
Cuts at: 3161 3419 3974 4203 5013 5144 5181 5492 5600
Size: 258 555 229 810 131 37 311 108
Cuts at: 5600 6377 6974 7761 8029 1239
Size: 777 597 787 268 1524
Fragments arranged by size:
1524 810 787 777 597 555 437 393
319 311 274 268 258 233 229 148
131 108 91 37 27
EcoO109I rG'GnC_Cy
Cuts at: 2036 2037 2116 3706 4016 4859 4860 2036
Size: 1 79 1590 310 843 1 5490
Fragments arranged by size:
5490 1590 843 310 79 1 1
EcoRI G'AATT_C
Cuts at: 5399 5623 6001 5399
Size: 224 378 7712
Fragments arranged by size:
7712 378 224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2126 4061 5315 2126
Size: 1935 1254 5125
Fragments arranged by size:
5125 1935 1254
FseI GG_CCGG'CC
Cuts at: 3226 3226
Size: 8314
FspI TGC'GCA
Cuts at: 2696 6820 2696
Size: 4124 4190
HaeII r_GCGC'y
Cuts at: 1678 3620 7461 1678
Size: 1942 3841 2531
Fragments arranged by size:
3841 2531 1942
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2942 2974 3100 3132 5261
Size: 32 135 32 1370 32 126 32 2129
Cuts at: 5261 5293 6955 6987 7029 7061 1373
Size: 32 1662 32 42 32 2626
Fragments arranged by size:
2626 2129 1662 1370 135 126 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2495 5144 5658 5904 669
Size: 988 281 557 2649 514 246 3079
Fragments arranged by size:
3079 2649 988 557 514 281 246
HindIII A'AGCT_T
Cuts at: 748 3991 748
Size: 3243 5071
HpaI GTT'AAC
Cuts at: 5904 5904
Size: 8314
KpnI G_GTAC'C
Cuts at: 5639 5639
Size: 8314
MfeI C'AATT_G
Cuts at: 5913 5913
Size: 8314
MluI A'CGCG_T
Cuts at: 8190 8190
Size: 8314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3297 4761 7428 7612 1305
Size: 1992 1464 2667 184 2007
Fragments arranged by size:
2667 2007 1992 1464 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2978 3053 3284 3387 3557
Size: 54 1425 343 1146 75 231 103 170
Cuts at: 3557 5411 10
Size: 1854 2913
Fragments arranged by size:
2913 1854 1425 1146 343 231 170 103
75 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3047 3748 4926 5078 5187 6272 6631
Size: 648 1881 701 1178 152 109 1085 359
Cuts at: 6631 6790 518
Size: 159 2042
Fragments arranged by size:
2042 1881 1178 1085 701 648 359 159
152 109
MspA1I CmG'CkG
Cuts at: 1606 2718 2739 3188 3725 5578 5730 6407 7555
Size: 1112 21 449 537 1853 152 677 1148
Cuts at: 7555 7800 1606
Size: 245 2120
Fragments arranged by size:
2120 1853 1148 1112 677 537 449 245
152 21
NaeI GCC'GGC
Cuts at: 3224 3224
Size: 8314
NcoI C'CATG_G
Cuts at: 513 1065 2022 3852 4594 5211 513
Size: 552 957 1830 742 617 3616
Fragments arranged by size:
3616 1830 957 742 617 552
NdeI CA'TA_TG
Cuts at: 387 5367 387
Size: 4980 3334
NgoMIV G'CCGG_C
Cuts at: 3222 3222
Size: 8314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8314
NotI GC'GGCC_GC
Cuts at: 5761 5761
Size: 8314
NspI r_CATG'y
Cuts at: 1132 1229 5238 5672 7217 1132
Size: 97 4009 434 1545 2229
Fragments arranged by size:
4009 2229 1545 434 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8314
PciI A'CATG_T
Cuts at: 5234 7213 5234
Size: 1979 6335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4759 1108
Size: 3651 4663
PfoI T'CCnGG_A
Cuts at: 2657 2946 2657
Size: 289 8025
PmeI GTTT'AAAC
Cuts at: 5618 5618
Size: 8314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 6383 6415 7896 7928 3023
Size: 32 3328 32 1481 32 3409
Fragments arranged by size:
3409 3328 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2116 2116
Size: 8314
PshAI GACnn'nnGTC
Cuts at: 1613 5178 1613
Size: 3565 4749
PsiI TTA'TAA
Cuts at: 5884 5884
Size: 8314
PspOMI G'GGCC_C
Cuts at: 2036 3705 4859 2036
Size: 1669 1154 5491
Fragments arranged by size:
5491 1669 1154
PstI C_TGCA'G
Cuts at: 830 2136 2723 3162 5666 830
Size: 1306 587 439 2504 3478
Fragments arranged by size:
3478 2504 1306 587 439
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8314
PvuII CAG'CTG
Cuts at: 2739 3188 3725 5578 2739
Size: 449 537 1853 5475
Fragments arranged by size:
5475 1853 537 449
SalI G'TCGA_C
Cuts at: 1936 2493 5142 5656 1936
Size: 557 2649 514 4594
Fragments arranged by size:
4594 2649 557 514
SapI GCTCTTCn'nnn_
Cuts at: 4157 4157
Size: 8314
SbfI CC_TGCA'GG
Cuts at: 5666 5666
Size: 8314
ScaI AGT'ACT
Cuts at: 3017 6562 8274 3017
Size: 3545 1712 3057
Fragments arranged by size:
3545 3057 1712
SexAI A'CCwGG_T
Cuts at: 2772 2772
Size: 8314
SfcI C'TryA_G
Cuts at: 826 1045 2132 2719 3158 4029 4380 5595 5662
Size: 219 1087 587 439 871 351 1215 67
Cuts at: 5662 6797 7478 7669 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1215 1135 1087 871 681 587 439
351 219 191 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 2984 2984
Size: 8314
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8314
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8314
SmaI CCC'GGG
Cuts at: 1872 2811 5641 1872
Size: 939 2830 4545
Fragments arranged by size:
4545 2830 939
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3464 4081 6418 7319 7581 820
Size: 197 926 1521 617 2337 901 262 1553
Fragments arranged by size:
2337 1553 1521 926 901 617 262 197
SnaBI TAC'GTA
Cuts at: 493 5611 6105 493
Size: 5118 494 2702
Fragments arranged by size:
5118 2702 494
SpeI A'CTAG_T
Cuts at: 152 2089 152
Size: 1937 6377
SphI G_CATG'C
Cuts at: 1132 5672 1132
Size: 4540 3774
SspI AAT'ATT
Cuts at: 5 52 3291 6238 5
Size: 47 3239 2947 2081
Fragments arranged by size:
3239 2947 2081 47
StuI AGG'CCT
Cuts at: 1517 3951 1517
Size: 2434 5880
StyI C'CwwG_G
Cuts at: 513 1065 2022 2064 2082 2988 3852 4317 4594
Size: 552 957 42 18 906 864 465 277
Cuts at: 4594 4753 5211 5753 513
Size: 159 458 542 3074
Fragments arranged by size:
3074 957 906 864 552 542 465 458
277 159 42 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2015 2456 3679 6341 6358 6511 6696
Size: 1255 435 441 1223 2662 17 153 185
Cuts at: 6696 7141 325
Size: 445 1498
Fragments arranged by size:
2662 1498 1255 1223 445 441 435 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 3015 4937
Size: 274 80 33 51 1443 75 963 1922
Cuts at: 4937 6560 8272 96
Size: 1623 1712 138
Fragments arranged by size:
1922 1712 1623 1443 963 274 138 80
75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2911 2943 3515 3547 4162 4194 8274 8306 2911
Size: 32 572 32 615 32 4080 32 2919
Fragments arranged by size:
4080 2919 615 572 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2222 3677 1316
Size: 906 1455 5953
Fragments arranged by size:
5953 1455 906
XbaI T'CTAG_A
Cuts at: 4498 5650 4498
Size: 1152 7162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2736 3292 3673 3812 2736
Size: 556 381 139 7238
Fragments arranged by size:
7238 556 381 139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8314
XmaI C'CCGG_G
Cuts at: 1870 2809 5639 1870
Size: 939 2830 4545
Fragments arranged by size:
4545 2830 939
XmnI GAAnn'nnTTC
Cuts at: 5538 6443 5538
Size: 905 7409
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6122 276
Size: 53 83 186 936 4588 2468
Fragments arranged by size:
4588 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoICRI EcoNI EcoRV FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SfoI SrfI SwaI