Restriction map for
pFN21ASDA0646
(Circular) (Six-base) MAPSORT of: pFN21ASDA0646.seq Check: 4105 from: 1 to: 6184
pFN21ASDA0646 6184 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3994 278
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3527 1937
Size: 1590 4594
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2418 2541 2724 2882 2978 3050 4247 5631
Size: 786 123 183 158 96 72 1197 1384
Cuts at: 5631 5899 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1197 786 268 183 158 123
96 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 5083 6060 1203
Size: 3880 977 1327
Fragments arranged by size:
3880 1327 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 2908 3355 4913 2908
Size: 447 1558 4179
Fragments arranged by size:
4179 1558 447
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6184
AlwNI CAG_nnn'CTG
Cuts at: 2513 3052 3288 3927 5499 2513
Size: 539 236 639 1572 3198
Fragments arranged by size:
3198 1572 639 539 236
ApaLI G'TGCA_C
Cuts at: 4241 5397 4241
Size: 1156 5028
ApoI r'AATT_y
Cuts at: 1493 2486 2533 3493 3719 3871 5980 1493
Size: 993 47 960 226 152 2109 1697
Fragments arranged by size:
2109 1697 993 960 226 152 47
AseI AT'TA_AT
Cuts at: 160 1032 3393 4738 160
Size: 872 2361 1345 1606
Fragments arranged by size:
2361 1606 1345 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 3509 1870
Size: 73 1566 4545
Fragments arranged by size:
4545 1566 73
BamHI G'GATC_C
Cuts at: 2632 3514 3881 2632
Size: 882 367 4935
Fragments arranged by size:
4935 882 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2076 3505 4960 618
Size: 325 217 627 289 1429 1455 1842
Fragments arranged by size:
1842 1455 1429 627 325 289 217
BanII G_rGCy'C
Cuts at: 1391 2034 2677 3070 3225 3419 3503 1391
Size: 643 643 393 155 194 84 4072
Fragments arranged by size:
4072 643 643 393 194 155 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2409 2972 928
Size: 971 510 563 4140
Fragments arranged by size:
4140 971 563 510
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4375 4409 1903
Size: 34 2438 34 3678
Fragments arranged by size:
3678 2438 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4076 5286 4076
Size: 1210 4974
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4795 136
Size: 107 122 71 631 605 3123 1525
Fragments arranged by size:
3123 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6179 6179
Size: 6184
BlpI GC'TnA_GC
Cuts at: 2934 3601 2934
Size: 667 5517
Bme1580I G_kGCm'C
Cuts at: 1790 3280 4245 5401 1790
Size: 1490 965 1156 2573
Fragments arranged by size:
2573 1490 1156 965
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2416 3915 4873
Size: 416 53 83 399 24 999 1499 958
Cuts at: 4873 442
Size: 1753
Fragments arranged by size:
1753 1499 999 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2535 4844 1239
Size: 1296 2309 2579
Fragments arranged by size:
2579 2309 1296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2672 4309 5174 5472 2672
Size: 1637 865 298 3384
Fragments arranged by size:
3384 1637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2046 4847 882
Size: 617 547 2801 2219
Fragments arranged by size:
2801 2219 617 547
BsaAI yAC'GTr
Cuts at: 493 1204 3481 3975 493
Size: 711 2277 494 2702
Fragments arranged by size:
2702 2277 711 494
BsaBI GATnn'nnATC
Cuts at: 6178 6178
Size: 6184
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3991 4373 5008 275
Size: 53 83 186 936 2458 382 635 1451
Fragments arranged by size:
2458 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4617 5015 5289 5436 5847 4617
Size: 398 274 147 411 4954
Fragments arranged by size:
4954 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2659 2689 1178
Size: 30 165 30 1256 30 4673
Fragments arranged by size:
4673 1256 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6184
BseYI C'CCAG_C
Cuts at: 2913 5387 5883 2913
Size: 2474 496 3214
Fragments arranged by size:
3214 2474 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2943 1163
Size: 573 1207 4404
Fragments arranged by size:
4404 1207 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3634 4395 5423 1540
Size: 275 186 1633 761 1028 2301
Fragments arranged by size:
2301 1633 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3225 3419 4245 4330 5401 3225
Size: 194 826 85 1071 4008
Fragments arranged by size:
4008 1071 826 194 85
BsmI GAATG_Cn'
Cuts at: 2377 3335 3695 3788 2377
Size: 958 360 93 4773
Fragments arranged by size:
4773 958 360 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2677 3070 3225 3280 3419 3503
Size: 399 244 643 393 155 55 139 84
Cuts at: 3503 4245 4330 5401 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 643 399 393 244 155
139 85 84 55
BspEI T'CCGG_A
Cuts at: 5847 5847
Size: 6184
BspHI T'CATG_A
Cuts at: 2716 4073 5803 2716
Size: 1357 1730 3097
Fragments arranged by size:
3097 1730 1357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2498 3539 844
Size: 1654 1041 3489
Fragments arranged by size:
3489 1654 1041
BsrBI CCG'CTC
Cuts at: 1394 1823 4071 1394
Size: 429 2248 3507
Fragments arranged by size:
3507 2248 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4679 4853 65
Size: 1183 3431 174 1396
Fragments arranged by size:
3431 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 4828 5015 1700
Size: 110 250 2768 187 2869
Fragments arranged by size:
2869 2768 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 4244 5256 4244
Size: 1012 5172
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6184
BstBI TT'CG_AA
Cuts at: 2007 4988 2007
Size: 2981 3203
BstEII G'GTnAC_C
Cuts at: 1184 2773 1184
Size: 1589 4595
BstYI r'GATC_y
Cuts at: 1972 2632 3514 3881 4266 4283 5735 5821 5833
Size: 660 882 367 385 17 1452 86 12
Cuts at: 5833 6179 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 882 660 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2232 2232
Size: 6184
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4675
Fragments arranged by size:
4675 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5445
Fragments arranged by size:
5445 683 56
BtsI GCAGTG_nn'
Cuts at: 3700 4494 4514 3700
Size: 794 20 5370
Fragments arranged by size:
5370 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6149
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5191 809
Size: 507 3875 1802
Fragments arranged by size:
3875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2065 2607 3631
Size: 54 1425 50 275 18 235 542 1024
Cuts at: 3631 4520 6149 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1024 889 542 275 235 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2743 2973 3072 3147 4114 1353
Size: 1390 230 99 75 967 3423
Fragments arranged by size:
3423 1390 967 230 99 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4771 5145 5291 387
Size: 1064 138 3182 374 146 1280
Fragments arranged by size:
3182 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3223 3417 3223
Size: 194 5990
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2418 2535 2541 2724 2882 2978 3050
Size: 393 786 117 6 183 158 96 72
Cuts at: 3050 4247 4844 5631 5899 1239
Size: 1197 597 787 268 1524
Fragments arranged by size:
1524 1197 787 786 597 393 268 183
158 117 96 72 6
EcoO109I rG'GnC_Cy
Cuts at: 2510 2525 3025 2510
Size: 15 500 5669
Fragments arranged by size:
5669 500 15
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3118 3206 3118
Size: 88 6096
FspI TGC'GCA
Cuts at: 4690 4690
Size: 6184
HaeII r_GCGC'y
Cuts at: 1678 2103 5331 1678
Size: 425 3228 2531
Fragments arranged by size:
3228 2531 425
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2142 2174 2185 2217 2322
Size: 32 135 32 570 32 11 32 105
Cuts at: 2322 2354 2404 2436 2894 2926 3341 3373 4825
Size: 32 50 32 458 32 415 32 1452
Cuts at: 4825 4857 4899 4931 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1452 570 458 415 135 105 50
42 32 32 32 32 32 32 32
32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2643 3528 3774 669
Size: 988 281 705 885 246 3079
Fragments arranged by size:
3079 988 885 705 281 246
HindIII A'AGCT_T
Cuts at: 748 2755 748
Size: 2007 4177
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 3385 3783 3385
Size: 398 5786
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2365 3323 5298 5482 1305
Size: 1060 958 1975 184 2007
Fragments arranged by size:
2007 1975 1060 958 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2609 10
Size: 54 1425 343 777 3585
Fragments arranged by size:
3585 1425 777 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3201 4142 4501 4660 518
Size: 648 2035 941 359 159 2042
Fragments arranged by size:
2042 2035 941 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2917 3040 3600 4277 5425 5670 1606
Size: 1311 123 560 677 1148 245 2120
Fragments arranged by size:
2120 1311 1148 677 560 245 123
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4675
Fragments arranged by size:
4675 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 3542 5087 1132
Size: 97 2313 1545 2229
Fragments arranged by size:
2313 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6184
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6184
PfoI T'CCnGG_A
Cuts at: 2512 2783 2973 2512
Size: 271 190 5723
Fragments arranged by size:
5723 271 190
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2510 3025 2510
Size: 515 5669
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6184
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3223 3255 3223
Size: 32 6152
PstI C_TGCA'G
Cuts at: 830 3057 3536 830
Size: 2227 479 3478
Fragments arranged by size:
3478 2227 479
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
PvuII CAG'CTG
Cuts at: 2917 3040 2917
Size: 123 6061
SacI G_AGCT'C
Cuts at: 3225 3419 3225
Size: 194 5990
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SapI GCTCTTCn'nnn_
Cuts at: 3072 3072
Size: 6184
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 4432 6144 4432
Size: 1712 4472
SfcI C'TryA_G
Cuts at: 826 1045 2139 3053 3532 4667 5348 5539 826
Size: 219 1094 914 479 1135 681 191 1471
Fragments arranged by size:
1471 1135 1094 914 681 479 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 2060 1700
Size: 360 5824
SmaI CCC'GGG
Cuts at: 1872 3511 1872
Size: 1639 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 4288 5189 5451 820
Size: 197 926 708 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 708 262 197
SnaBI TAC'GTA
Cuts at: 493 3481 3975 493
Size: 2988 494 2702
Fragments arranged by size:
2988 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 4108 5
Size: 47 4056 2081
Fragments arranged by size:
4056 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6184
StyI C'CwwG_G
Cuts at: 513 1065 2022 3013 3147 3623 513
Size: 552 957 991 134 476 3074
Fragments arranged by size:
3074 991 957 552 476 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2115 4211 4228 4381 4566 5011 325
Size: 1255 535 2096 17 153 185 445 1498
Fragments arranged by size:
2096 1498 1255 535 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2612 4430 6142
Size: 274 80 33 51 1443 635 1818 1712
Cuts at: 6142 96
Size: 138
Fragments arranged by size:
1818 1712 1443 635 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 2659 2691 6144 6176 2111
Size: 32 516 32 3453 32 2119
Fragments arranged by size:
3453 2119 516 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2779 1316
Size: 1463 4721
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 3509 1870
Size: 1639 4545
XmnI GAAnn'nnTTC
Cuts at: 2969 3186 4313 2969
Size: 217 1127 4840
Fragments arranged by size:
4840 1127 217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3992 276
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI