Restriction map for
pFN21ASDA0645
(Circular) (Six-base) MAPSORT of: pFN21ASDA0645.seq Check: 3174 from: 1 to: 9445
pFN21ASDA0645 9445 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3470 3648 3470
Size: 178 9267
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7255 278
Size: 53 83 186 936 5719 2468
Fragments arranged by size:
5719 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2040 2292 6788 1937
Size: 103 252 4496 4594
Fragments arranged by size:
4594 4496 252 103
Acc65I G'GTAC_C
Cuts at: 6766 6766
Size: 9445
AclI AA'CG_TT
Cuts at: 1563 4571 7572 7945 1563
Size: 3008 3001 373 3063
Fragments arranged by size:
3063 3008 3001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4060 4610 4777 4806 4832 6668 7508 8892
Size: 2428 550 167 29 26 1836 840 1384
Cuts at: 8892 9160 1632
Size: 268 1917
Fragments arranged by size:
2428 1917 1836 1384 840 550 268 167
29 26
AflII C'TTAA_G
Cuts at: 820 1017 2195 820
Size: 197 1178 8070
Fragments arranged by size:
8070 1178 197
AflIII A'CryG_T
Cuts at: 1203 2393 6540 8344 9321 1203
Size: 1190 4147 1804 977 1327
Fragments arranged by size:
4147 1804 1327 1190 977
AgeI A'CCGG_T
Cuts at: 8276 8276
Size: 9445
AhdI GACnn_n'nnGTC
Cuts at: 4060 4228 8174 4060
Size: 168 3946 5331
Fragments arranged by size:
5331 3946 168
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3488 6747 1184
Size: 2304 3259 3882
Fragments arranged by size:
3882 3259 2304
AlwNI CAG_nnn'CTG
Cuts at: 3513 4291 4671 5425 7188 8760 3513
Size: 778 380 754 1763 1572 4198
Fragments arranged by size:
4198 1763 1572 778 754 380
ApaI G_GGCC'C
Cuts at: 3448 5055 3448
Size: 1607 7838
ApaLI G'TGCA_C
Cuts at: 3732 5840 7502 8658 3732
Size: 2108 1662 1156 4519
Fragments arranged by size:
4519 2108 1662 1156
ApoI r'AATT_y
Cuts at: 1493 2720 4715 6754 6980 7132 9241 1493
Size: 1227 1995 2039 226 152 2109 1697
Fragments arranged by size:
2109 2039 1995 1697 1227 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7999 160
Size: 872 6967 1606
Fragments arranged by size:
6967 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9445
AvaI C'yCGr_G
Cuts at: 1870 1943 3035 3350 3441 3684 6770 1870
Size: 73 1092 315 91 243 3086 4545
Fragments arranged by size:
4545 3086 1092 315 243 91 73
AvrII C'CTAG_G
Cuts at: 3598 4136 3598
Size: 538 8907
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2490 2523 5552 5585 2490
Size: 33 3029 33 6350
Fragments arranged by size:
6350 3029 33 33
BamHI G'GATC_C
Cuts at: 3970 5589 6775 7142 3970
Size: 1619 1186 367 6273
Fragments arranged by size:
6273 1619 1186 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5297 6766 8221 618
Size: 325 217 627 3510 1469 1455 1842
Fragments arranged by size:
3510 1842 1469 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3448 5055 5195 5727 5838 6764 1391
Size: 2057 1607 140 532 111 926 4072
Fragments arranged by size:
4072 2057 1607 926 532 140 111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2784 4794 928
Size: 971 885 2010 5579
Fragments arranged by size:
5579 2010 971 885
BbvCI CC'TCA_GC
Cuts at: 2923 5110 2923
Size: 2187 7258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7636 7670 1903
Size: 34 5699 34 3678
Fragments arranged by size:
5699 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2874 3665 3785 4641 7337 8547 2874
Size: 791 120 856 2696 1210 3772
Fragments arranged by size:
3772 2696 1210 856 791 120
BclI T'GATC_A
Cuts at: 1548 3027 4602 1548
Size: 1479 1575 6391
Fragments arranged by size:
6391 1575 1479
BfrBI ATG'CAT
Cuts at: 5657 6724 5657
Size: 1067 8378
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5825 5884 8056
Size: 107 122 71 631 605 4153 59 2172
Cuts at: 8056 136
Size: 1525
Fragments arranged by size:
4153 2172 1525 631 605 122 107 71
59
BglII A'GATC_T
Cuts at: 3467 9440 3467
Size: 5973 3472
BlpI GC'TnA_GC
Cuts at: 4109 4484 6862 4109
Size: 375 2378 6692
Fragments arranged by size:
6692 2378 375
Bme1580I G_kGCm'C
Cuts at: 1790 3448 3736 5055 5844 6241 6736 7506 8662
Size: 1658 288 1319 789 397 495 770 1156
Cuts at: 8662 1790
Size: 2573
Fragments arranged by size:
2573 1658 1319 1156 789 770 495 397
288
BmgBI CAC'GTC
Cuts at: 1318 6007 1318
Size: 4689 4756
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3018 3442 5058
Size: 416 53 83 399 24 1601 424 1616
Cuts at: 5058 5084 5194 6176 6204 6334 6580 7176 8134
Size: 26 110 982 28 130 246 596 958
Cuts at: 8134 442
Size: 1753
Fragments arranged by size:
1753 1616 1601 982 958 596 424 416
399 246 130 110 83 53 28 26
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9445
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4717 4717
Size: 9445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2963 3712 3716 4019 4167 4754 4840 5093
Size: 1724 749 4 303 148 587 86 253
Cuts at: 5093 5386 6050 6592 6734 8105 1239
Size: 293 664 542 142 1371 2579
Fragments arranged by size:
2579 1724 1371 749 664 587 542 303
293 253 148 142 86 4
Bpu10I CC'TnA_GC
Cuts at: 2923 5110 2923
Size: 2187 7258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2229 2889 3399 6260 7570 8435 8733 2229
Size: 660 510 2861 1310 865 298 2941
Fragments arranged by size:
2941 2861 1310 865 660 510 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2254 8108 882
Size: 617 755 5854 2219
Fragments arranged by size:
5854 2219 755 617
BsaAI yAC'GTr
Cuts at: 493 1204 5581 6742 7236 493
Size: 711 4377 1161 494 2702
Fragments arranged by size:
4377 2702 1161 711 494
BsaBI GATnn'nnATC
Cuts at: 9439 9439
Size: 9445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3402 7252 7634 8269
Size: 53 83 186 936 1869 3850 382 635
Cuts at: 8269 275
Size: 1451
Fragments arranged by size:
3850 1869 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3899 7878 8276 8550 8697 9108 3899
Size: 3979 398 274 147 411 4236
Fragments arranged by size:
4236 3979 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2121 2151 3578 3608 4532
Size: 30 165 30 718 30 1427 30 924
Cuts at: 4532 4562 4730 4760 5266 5296 1178
Size: 30 168 30 506 30 5327
Fragments arranged by size:
5327 1427 924 718 506 168 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3586 3973 4103 4550 4553 4751 5380 6566
Size: 2400 387 130 447 3 198 629 1186
Cuts at: 6566 1186
Size: 4065
Fragments arranged by size:
4065 2400 1186 629 447 387 198 130
3
BseYI C'CCAG_C
Cuts at: 4380 4707 6240 8648 9144 4380
Size: 327 1533 2408 496 4681
Fragments arranged by size:
4681 2408 1533 496 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2819 3626 3780 4143 4388 4460 4938
Size: 573 1083 807 154 363 245 72 478
Cuts at: 4938 5067 6058 6077 1163
Size: 129 991 19 4531
Fragments arranged by size:
4531 1083 991 807 573 478 363 245
154 129 72 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5731 6895 7656 8684 1540
Size: 275 186 3730 1164 761 1028 2301
Fragments arranged by size:
3730 2301 1164 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2440 3184 3736 3811 5195 5838 5844 6294 7506
Size: 744 552 75 1384 643 6 450 1212
Cuts at: 7506 7591 8662 2440
Size: 85 1071 3223
Fragments arranged by size:
3223 1384 1212 1071 744 643 552 450
85 75 6
BsiWI C'GTAC_G
Cuts at: 6738 6738
Size: 9445
BsmI GAATG_Cn'
Cuts at: 4980 5065 6076 6339 6956 7049 4980
Size: 85 1011 263 617 93 7376
Fragments arranged by size:
7376 1011 617 263 93 85
BsmBI CGTCTCn'nnnn_
Cuts at: 4418 4705 4418
Size: 287 9158
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2440 3184 3448 3736 3811 5055 5195
Size: 399 650 744 264 288 75 1244 140
Cuts at: 5195 5727 5838 5844 6241 6294 6736 6764 7506
Size: 532 111 6 397 53 442 28 742
Cuts at: 7506 7591 8662 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1244 1071 744 742 650 532 442
399 397 288 264 140 111 85 75
53 28 6
BspEI T'CCGG_A
Cuts at: 3899 9108 3899
Size: 5209 4236
BspHI T'CATG_A
Cuts at: 2662 3069 4012 7334 9064 2662
Size: 407 943 3322 1730 3043
Fragments arranged by size:
3322 3043 1730 943 407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2173 3470 3648 4115 4375 6800 844
Size: 1329 1297 178 467 260 2425 3489
Fragments arranged by size:
3489 2425 1329 1297 467 260 178
BsrBI CCG'CTC
Cuts at: 1394 1823 6556 7332 1394
Size: 429 4733 776 3507
Fragments arranged by size:
4733 3507 776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6354 7940 8114 65
Size: 1183 5106 1586 174 1396
Fragments arranged by size:
5106 1586 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4778 5883 6024 6684 8089 8276 1700
Size: 110 2968 1105 141 660 1405 187 2869
Fragments arranged by size:
2968 2869 1405 1105 660 187 141 110
BsrGI T'GTAC_A
Cuts at: 96 6376 96
Size: 6280 3165
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9445
BssSI C'ACGA_G
Cuts at: 5837 7505 8517 5837
Size: 1668 1012 6765
Fragments arranged by size:
6765 1668 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5629 794
Size: 4835 4610
BstBI TT'CG_AA
Cuts at: 2007 8249 2007
Size: 6242 3203
BstEII G'GTnAC_C
Cuts at: 1184 6106 1184
Size: 4922 4523
BstXI CCAn_nnnn'nTGG
Cuts at: 5242 5354 5242
Size: 112 9333
BstYI r'GATC_y
Cuts at: 1972 3467 3915 3970 4650 5466 5589 6775 7142
Size: 1495 448 55 680 816 123 1186 367
Cuts at: 7142 7527 7544 8996 9082 9094 9440 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1495 1452 1186 816 680 448 385
367 346 123 86 55 17 12
Bsu36I CC'TnA_GG
Cuts at: 2298 4533 2298
Size: 2235 7210
BtgI C'CryG_G
Cuts at: 513 1065 2353 5328 6052 513
Size: 552 1288 2975 724 3906
Fragments arranged by size:
3906 2975 1288 724 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3219 4979 5618 487
Size: 683 56 1993 1760 639 4314
Fragments arranged by size:
4314 1993 1760 683 639 56
BtsI GCAGTG_nn'
Cuts at: 2083 3551 3763 4888 6203 6961 7755 7775 2083
Size: 1468 212 1125 1315 758 794 20 3753
Fragments arranged by size:
3753 1468 1315 1125 794 758 212 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9410
DraI TTT'AAA
Cuts at: 6749 6749
Size: 9445
DraIII CAC_nnn'GTG
Cuts at: 4582 4582
Size: 9445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8452 809
Size: 507 7136 1802
Fragments arranged by size:
7136 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2350 3334 5607
Size: 54 1425 50 275 18 520 984 2273
Cuts at: 5607 5736 5885 6892 7781 9410 8
Size: 129 149 1007 889 1629 43
Fragments arranged by size:
2273 1629 1425 1007 984 889 520 275
149 129 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6892 1537
Size: 275 5080 4090
Fragments arranged by size:
5080 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2806 2858 4389 5183 5631 5826 6128 6251
Size: 1453 52 1531 794 448 195 302 123
Cuts at: 6251 6347 7375 1353
Size: 96 1028 3423
Fragments arranged by size:
3423 1531 1453 1028 794 448 302 195
123 96 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3959 5185 5689 6526 8032 8406
Size: 1064 138 2370 1226 504 837 1506 374
Cuts at: 8406 8552 387
Size: 146 1280
Fragments arranged by size:
2370 1506 1280 1226 1064 837 504 374
146 138
EcoICRI GAG'CTC
Cuts at: 5193 5836 5193
Size: 643 8802
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2963 3712 3716 4019 4060 4167 4610
Size: 393 1331 749 4 303 41 107 443
Cuts at: 4610 4754 4777 4806 4832 4840 5093 5386 6050
Size: 144 23 29 26 8 253 293 664
Cuts at: 6050 6592 6668 6734 7508 8105 8892 9160 1239
Size: 542 76 66 774 597 787 268 1524
Fragments arranged by size:
1524 1331 787 774 749 664 597 542
443 393 303 293 268 253 144 107
76 66 41 29 26 23 8 4
EcoNI CCTnn'n_nnAGG
Cuts at: 6344 6344
Size: 9445
EcoO109I rG'GnC_Cy
Cuts at: 4140 4288 4824 4911 5524 6194 4140
Size: 148 536 87 613 670 7391
Fragments arranged by size:
7391 670 613 536 148 87
EcoRI G'AATT_C
Cuts at: 4715 6754 7132 4715
Size: 2039 378 7028
Fragments arranged by size:
7028 2039 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 9445
FspI TGC'GCA
Cuts at: 7951 7951
Size: 9445
HaeII r_GCGC'y
Cuts at: 1678 3871 6607 6615 8592 1678
Size: 2193 2736 8 1977 2531
Fragments arranged by size:
2736 2531 2193 1977 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2227 2251 2259 2283 2693
Size: 32 135 32 655 24 8 24 410
Cuts at: 2693 2725 3478 3509 3510 3541 3578 3610 3894
Size: 32 753 31 1 31 37 32 284
Cuts at: 3894 3926 4046 4078 4214 4246 5399 5431 8086
Size: 32 120 32 136 32 1153 32 2655
Cuts at: 8086 8118 8160 8192 1373
Size: 32 42 32 2626
Fragments arranged by size:
2655 2626 1153 753 655 410 284 136
135 120 42 37 32 32 32 32
32 32 32 32 32 32 31 31
24 24 8 1
HincII GTy'rAC
Cuts at: 669 1657 1938 5079 6004 6789 7035 669
Size: 988 281 3141 925 785 246 3079
Fragments arranged by size:
3141 3079 988 925 785 281 246
HindIII A'AGCT_T
Cuts at: 748 2123 3848 748
Size: 1375 1725 6345
Fragments arranged by size:
6345 1725 1375
HpaI GTT'AAC
Cuts at: 7035 7035
Size: 9445
KpnI G_GTAC'C
Cuts at: 6770 6770
Size: 9445
MfeI C'AATT_G
Cuts at: 7044 7044
Size: 9445
MluI A'CGCG_T
Cuts at: 9321 9321
Size: 9445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3882 3998 4195 5109 5271 8559 8743 1305
Size: 2577 116 197 914 162 3288 184 2007
Fragments arranged by size:
3288 2577 2007 914 197 184 162 116
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5609 5738 10
Size: 54 1425 343 3777 129 3717
Fragments arranged by size:
3777 3717 1425 343 129 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3620 3648 5240 5285 6729 7403 7762
Size: 648 2454 28 1592 45 1444 674 359
Cuts at: 7762 7921 518
Size: 159 2042
Fragments arranged by size:
2454 2042 1592 1444 674 648 359 159
45 28
MspA1I CmG'CkG
Cuts at: 1606 3088 3960 4483 5174 5425 5506 6042 6267
Size: 1482 872 523 691 251 81 536 225
Cuts at: 6267 6466 6502 6861 7538 8686 8931 1606
Size: 199 36 359 677 1148 245 2120
Fragments arranged by size:
2120 1482 1148 872 691 677 536 523
359 251 245 225 199 81 36
NaeI GCC'GGC
Cuts at: 4780 5885 4780
Size: 1105 8340
NcoI C'CATG_G
Cuts at: 513 1065 5328 6052 513
Size: 552 4263 724 3906
Fragments arranged by size:
4263 3906 724 552
NdeI CA'TA_TG
Cuts at: 387 5312 387
Size: 4925 4520
NgoMIV G'CCGG_C
Cuts at: 4778 5883 4778
Size: 1105 8340
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9445
NotI GC'GGCC_GC
Cuts at: 6892 6892
Size: 9445
NsiI A_TGCA'T
Cuts at: 5659 6726 5659
Size: 1067 8378
NspI r_CATG'y
Cuts at: 1132 1229 2397 3149 3779 4063 5657 6544 6728
Size: 97 1168 752 630 284 1594 887 184
Cuts at: 6728 6803 8348 1132
Size: 75 1545 2229
Fragments arranged by size:
2229 1594 1545 1168 887 752 630 284
184 97 75
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9445
PciI A'CATG_T
Cuts at: 2393 6540 8344 2393
Size: 4147 1804 3494
Fragments arranged by size:
4147 3494 1804
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2416 5329 5760 6600 1108
Size: 1308 2913 431 840 3953
Fragments arranged by size:
3953 2913 1308 840 431
PfoI T'CCnGG_A
Cuts at: 3693 4941 5469 6345 3693
Size: 1248 528 876 6793
Fragments arranged by size:
6793 1248 876 528
PmeI GTTT'AAAC
Cuts at: 6749 6749
Size: 9445
PmlI CAC'GTG
Cuts at: 5581 5581
Size: 9445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5098 5130 7514 7546 9027 9059 5098
Size: 32 2384 32 1481 32 5484
Fragments arranged by size:
5484 2384 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4140 4824 4140
Size: 684 8761
PshAI GACnn'nnGTC
Cuts at: 1613 2675 5498 1613
Size: 1062 2823 5560
Fragments arranged by size:
5560 2823 1062
PsiI TTA'TAA
Cuts at: 7015 7015
Size: 9445
PspOMI G'GGCC_C
Cuts at: 3444 5051 3444
Size: 1607 7838
PstI C_TGCA'G
Cuts at: 830 3467 3991 4296 6465 6797 830
Size: 2637 524 305 2169 332 3478
Fragments arranged by size:
3478 2637 2169 524 332 305
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9445
PvuII CAG'CTG
Cuts at: 5174 5425 5506 6042 6267 6466 5174
Size: 251 81 536 225 199 8153
Fragments arranged by size:
8153 536 251 225 199 81
SacI G_AGCT'C
Cuts at: 5195 5838 5195
Size: 643 8802
SalI G'TCGA_C
Cuts at: 1936 6787 1936
Size: 4851 4594
SapI GCTCTTCn'nnn_
Cuts at: 5183 5631 5826 6251 5183
Size: 448 195 425 8377
Fragments arranged by size:
8377 448 425 195
SbfI CC_TGCA'GG
Cuts at: 6797 6797
Size: 9445
ScaI AGT'ACT
Cuts at: 6120 7693 9405 6120
Size: 1573 1712 6160
Fragments arranged by size:
6160 1712 1573
SexAI A'CCwGG_T
Cuts at: 3784 3784
Size: 9445
SfcI C'TryA_G
Cuts at: 826 1045 2656 3463 3987 4292 4501 4663 6461
Size: 219 1611 807 524 305 209 162 1798
Cuts at: 6461 6793 7928 8609 8800 826
Size: 332 1135 681 191 1471
Fragments arranged by size:
1798 1611 1471 1135 807 681 524 332
305 219 209 191 162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9445
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9445
SmaI CCC'GGG
Cuts at: 1872 3037 3443 6772 1872
Size: 1165 406 3329 4545
Fragments arranged by size:
4545 3329 1165 406
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2195 2244 2904 3414 3684 6275
Size: 197 926 252 49 660 510 270 2591
Cuts at: 6275 7549 8450 8712 820
Size: 1274 901 262 1553
Fragments arranged by size:
2591 1553 1274 926 901 660 510 270
262 252 197 49
SnaBI TAC'GTA
Cuts at: 493 6742 7236 493
Size: 6249 494 2702
Fragments arranged by size:
6249 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9445
SphI G_CATG'C
Cuts at: 1132 6728 6803 1132
Size: 5596 75 3774
Fragments arranged by size:
5596 3774 75
SspI AAT'ATT
Cuts at: 5 52 6389 7369 5
Size: 47 6337 980 2081
Fragments arranged by size:
6337 2081 980 47
StuI AGG'CCT
Cuts at: 1517 4512 1517
Size: 2995 6450
StyI C'CwwG_G
Cuts at: 513 1065 3598 3666 4136 5328 6052 6136 6884
Size: 552 2533 68 470 1192 724 84 748
Cuts at: 6884 513
Size: 3074
Fragments arranged by size:
3074 2533 1192 748 724 552 470 84
68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2423 3018 3032 4699 5745 7472 7489
Size: 1255 843 595 14 1667 1046 1727 17
Cuts at: 7489 7642 7827 8272 325
Size: 153 185 445 1498
Fragments arranged by size:
1727 1667 1498 1255 1046 843 595 445
185 153 17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4740 6118 6376
Size: 274 80 33 51 1443 2763 1378 258
Cuts at: 6376 7691 9403 96
Size: 1315 1712 138
Fragments arranged by size:
2763 1712 1443 1378 1315 274 258 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3096 3107 3128 3139 4022 4054 4730 4762 9405
Size: 11 21 11 883 32 676 32 4643
Cuts at: 9405 9437 3096
Size: 32 3104
Fragments arranged by size:
4643 3104 883 676 32 32 32 21
11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9445
XbaI T'CTAG_A
Cuts at: 2687 4159 6781 2687
Size: 1472 2622 5351
Fragments arranged by size:
5351 2622 1472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5068 5287 5068
Size: 219 9226
XhoI C'TCGA_G
Cuts at: 1943 3684 1943
Size: 1741 7704
XmaI C'CCGG_G
Cuts at: 1870 3035 3441 6770 1870
Size: 1165 406 3329 4545
Fragments arranged by size:
4545 3329 1165 406
XmnI GAAnn'nnTTC
Cuts at: 2615 6542 6653 7574 2615
Size: 3927 111 921 4486
Fragments arranged by size:
4486 3927 921 111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7253 276
Size: 53 83 186 936 5719 2468
Fragments arranged by size:
5719 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BstZ17I EcoRV FseI FspAI
KasI NarI NruI PacI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI