Restriction map for
pFN21ASDA0642
(Circular) (Six-base) MAPSORT of: pFN21ASDA0642.seq Check: 5923 from: 1 to: 8689
pFN21ASDA0642 8689 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3966 3966
Size: 8689
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2845 6499 278
Size: 53 83 186 936 1309 3654 2468
Fragments arranged by size:
3654 2468 1309 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3337 6032 1937
Size: 1400 2695 4594
Fragments arranged by size:
4594 2695 1400
Acc65I G'GTAC_C
Cuts at: 2266 5075 6010 2266
Size: 2809 935 4945
Fragments arranged by size:
4945 2809 935
AclI AA'CG_TT
Cuts at: 1563 6816 7189 1563
Size: 5253 373 3063
Fragments arranged by size:
5253 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2184 2988 3219 3330 3393 4151 4247 4994
Size: 552 804 231 111 63 758 96 747
Cuts at: 4994 5055 5471 6752 8136 8404 1632
Size: 61 416 1281 1384 268 1917
Fragments arranged by size:
1917 1384 1281 804 758 747 552 416
268 231 111 96 63 61
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8492
AflIII A'CryG_T
Cuts at: 1203 7588 8565 1203
Size: 6385 977 1327
Fragments arranged by size:
6385 1327 977
AgeI A'CCGG_T
Cuts at: 7520 7520
Size: 8689
AhdI GACnn_n'nnGTC
Cuts at: 3244 7418 3244
Size: 4174 4515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2501 4506 1184
Size: 1317 2005 5367
Fragments arranged by size:
5367 2005 1317
AlwNI CAG_nnn'CTG
Cuts at: 2044 3394 3530 3958 4148 4247 5710 6432 8004
Size: 1350 136 428 190 99 1463 722 1572
Cuts at: 8004 2044
Size: 2729
Fragments arranged by size:
2729 1572 1463 1350 722 428 190 136
99
ApaI G_GGCC'C
Cuts at: 4639 4639
Size: 8689
ApaLI G'TGCA_C
Cuts at: 3600 6746 7902 3600
Size: 3146 1156 4387
Fragments arranged by size:
4387 3146 1156
ApoI r'AATT_y
Cuts at: 1493 2219 2629 2672 3717 4386 5212 5353 5998
Size: 726 410 43 1045 669 826 141 645
Cuts at: 5998 6224 6376 8485 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1045 826 726 669 645 410
226 152 141 43
AseI AT'TA_AT
Cuts at: 160 1032 7243 160
Size: 872 6211 1606
Fragments arranged by size:
6211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8689
AvaI C'yCGr_G
Cuts at: 1870 1943 4638 6014 1870
Size: 73 2695 1376 4545
Fragments arranged by size:
4545 2695 1376 73
AvrII C'CTAG_G
Cuts at: 5348 5348
Size: 8689
BamHI G'GATC_C
Cuts at: 6019 6386 6019
Size: 367 8322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 4980 5075 6010 7465
Size: 325 217 627 479 2714 95 935 1455
Cuts at: 7465 618
Size: 1842
Fragments arranged by size:
2714 1842 1455 935 627 479 325 217
95
BanII G_rGCy'C
Cuts at: 1391 2074 2371 4639 5173 5228 6008 1391
Size: 683 297 2268 534 55 780 4072
Fragments arranged by size:
4072 2268 780 683 534 297 55
BbeI G_GCGC'C
Cuts at: 4984 4984
Size: 8689
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4023 4378 4555 928
Size: 971 2124 355 177 5062
Fragments arranged by size:
5062 2124 971 355 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6880 6914 1903
Size: 34 4943 34 3678
Fragments arranged by size:
4943 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6581 7791 6581
Size: 1210 7479
BclI T'GATC_A
Cuts at: 1548 4099 1548
Size: 2551 6138
BfrBI ATG'CAT
Cuts at: 2488 3673 5933 2488
Size: 1185 2260 5244
Fragments arranged by size:
5244 2260 1185
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2062 7300 136
Size: 107 122 71 631 605 390 5238 1525
Fragments arranged by size:
5238 1525 631 605 390 122 107 71
BglII A'GATC_T
Cuts at: 8684 8684
Size: 8689
BlpI GC'TnA_GC
Cuts at: 6106 6106
Size: 8689
Bme1580I G_kGCm'C
Cuts at: 1790 3354 3604 4639 6750 7906 1790
Size: 1564 250 1035 2111 1156 2573
Fragments arranged by size:
2573 2111 1564 1156 1035 250
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5901 6420 7378
Size: 416 53 83 399 24 4484 519 958
Cuts at: 7378 442
Size: 1753
Fragments arranged by size:
4484 1753 958 519 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3106 3106
Size: 8689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2097 2219 3018 3849 4766 5114 7349 1239
Size: 858 122 799 831 917 348 2235 2579
Fragments arranged by size:
2579 2235 917 858 831 799 348 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5681 6814 7679 7977 5681
Size: 1133 865 298 6393
Fragments arranged by size:
6393 1133 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2617 3551 7352 882
Size: 617 1118 934 3801 2219
Fragments arranged by size:
3801 2219 1118 934 617
BsaAI yAC'GTr
Cuts at: 493 1204 5986 6480 493
Size: 711 4782 494 2702
Fragments arranged by size:
4782 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3622 8683 3622
Size: 5061 3628
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2842 4981 6496 6878
Size: 53 83 186 936 1309 2139 1515 382
Cuts at: 6878 7513 275
Size: 635 1451
Fragments arranged by size:
2139 1515 1451 1309 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4803 7122 7520 7794 7941 8352 4803
Size: 2319 398 274 147 411 5140
Fragments arranged by size:
5140 2319 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2086 2116 2343 2373 3406
Size: 30 165 30 683 30 227 30 1033
Cuts at: 3406 3436 5227 5257 1178
Size: 30 1791 30 4610
Fragments arranged by size:
4610 1791 1033 683 227 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2094 2358 2361 2364 2484 3098 3329 3414
Size: 908 264 3 3 120 614 231 85
Cuts at: 3414 3873 4576 4634 4685 4709 1186
Size: 459 703 58 51 24 5166
Fragments arranged by size:
5166 908 703 614 459 264 231 120
85 58 51 24 3 3
BseYI C'CCAG_C
Cuts at: 2502 2599 3353 4035 4656 7892 8388 2502
Size: 97 754 682 621 3236 496 2803
Fragments arranged by size:
3236 2803 754 682 621 496 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5180 5879 1163
Size: 573 3444 699 3973
Fragments arranged by size:
3973 3444 699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6139 6900 7928 1540
Size: 275 186 4138 761 1028 2301
Fragments arranged by size:
4138 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2074 3092 3502 3604 3642 5173 6750 6835 7906
Size: 1018 410 102 38 1531 1577 85 1071
Cuts at: 7906 2074
Size: 2857
Fragments arranged by size:
2857 1577 1531 1071 1018 410 102 85
38
BsmI GAATG_Cn'
Cuts at: 3002 5185 6200 6293 3002
Size: 2183 1015 93 5398
Fragments arranged by size:
5398 2183 1015 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5960 5960
Size: 8689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2371 3092 3354 3502 3604 3642
Size: 399 284 297 721 262 148 102 38
Cuts at: 3642 4639 5173 5228 6008 6750 6835 7906 1391
Size: 997 534 55 780 742 85 1071 2174
Fragments arranged by size:
2174 1071 997 780 742 721 534 399
297 284 262 148 102 85 55 38
BspEI T'CCGG_A
Cuts at: 4803 8352 4803
Size: 3549 5140
BspHI T'CATG_A
Cuts at: 3834 6578 8308 3834
Size: 2744 1730 4215
Fragments arranged by size:
4215 2744 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3966 5202 6044 844
Size: 3122 1236 842 3489
Fragments arranged by size:
3489 3122 1236 842
BsrBI CCG'CTC
Cuts at: 1394 1823 6576 1394
Size: 429 4753 3507
Fragments arranged by size:
4753 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2117 3687 7184 7358 65
Size: 1183 869 1570 3497 174 1396
Fragments arranged by size:
3497 1570 1396 1183 869 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4677 5412 7333 7520 1700
Size: 110 2867 735 1921 187 2869
Fragments arranged by size:
2869 2867 1921 735 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8689
BssSI C'ACGA_G
Cuts at: 6749 7761 6749
Size: 1012 7677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3958 5710 794
Size: 3164 1752 3773
Fragments arranged by size:
3773 3164 1752
BstBI TT'CG_AA
Cuts at: 2007 7493 2007
Size: 5486 3203
BstEII G'GTnAC_C
Cuts at: 1184 2888 1184
Size: 1704 6985
BstXI CCAn_nnnn'nTGG
Cuts at: 3824 3824
Size: 8689
BstYI r'GATC_y
Cuts at: 1972 3703 4216 5097 5902 6019 6386 6771 6788
Size: 1731 513 881 805 117 367 385 17
Cuts at: 6788 8240 8326 8338 8684 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1731 1452 881 805 513 385 367
346 117 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3708 3708
Size: 8689
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 7180
Fragments arranged by size:
7180 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7950
Fragments arranged by size:
7950 683 56
BtsI GCAGTG_nn'
Cuts at: 2917 3520 5337 5501 6205 6999 7019 2917
Size: 603 1817 164 704 794 20 4587
Fragments arranged by size:
4587 1817 794 704 603 164 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8654
DraI TTT'AAA
Cuts at: 5993 5993
Size: 8689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2793 7696 809
Size: 507 1477 4903 1802
Fragments arranged by size:
4903 1802 1477 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3473 6136 7025
Size: 54 1425 50 275 18 1643 2663 889
Cuts at: 7025 8654 8
Size: 1629 43
Fragments arranged by size:
2663 1643 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6136 1537
Size: 275 4324 4090
Fragments arranged by size:
4324 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3039 3111 3306 3432 3788 4303 4332 4338
Size: 1686 72 195 126 356 515 29 6
Cuts at: 4338 4452 4506 4551 4561 4566 4611 4686 4727
Size: 114 54 45 10 5 45 75 41
Cuts at: 4727 4965 5013 5094 5103 6619 1353
Size: 238 48 81 9 1516 3423
Fragments arranged by size:
3423 1686 1516 515 356 238 195 126
114 81 75 72 54 48 45 45
41 29 10 9 6 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4600 7276 7650 7796 387
Size: 1064 138 3011 2676 374 146 1280
Fragments arranged by size:
3011 2676 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2072 5171 2072
Size: 3099 5590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2184 2219 2988 3018 3219 3330
Size: 393 465 87 35 769 30 201 111
Cuts at: 3330 3393 3849 4151 4247 4766 4994 5055 5114
Size: 63 456 302 96 519 228 61 59
Cuts at: 5114 5471 6752 7349 8136 8404 1239
Size: 357 1281 597 787 268 1524
Fragments arranged by size:
1524 1281 787 769 597 519 465 456
393 357 302 268 228 201 111 96
87 63 61 59 35 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2237 2237
Size: 8689
EcoO109I rG'GnC_Cy
Cuts at: 3725 4244 5345 5610 3725
Size: 519 1101 265 6804
Fragments arranged by size:
6804 1101 519 265
EcoRI G'AATT_C
Cuts at: 5212 5353 5998 6376 5212
Size: 141 645 378 7525
Fragments arranged by size:
7525 645 378 141
EcoRV GAT'ATC
Cuts at: 3832 3832
Size: 8689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4454 4559 4454
Size: 105 8584
FspI TGC'GCA
Cuts at: 7195 7195
Size: 8689
HaeII r_GCGC'y
Cuts at: 1678 4984 5668 7836 1678
Size: 3306 684 2168 2531
Fragments arranged by size:
3306 2531 2168 684
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3230 3262 3404 3436 4136
Size: 32 135 32 1658 32 142 32 700
Cuts at: 4136 4168 4990 5022 5910 5942 7330 7362 7404
Size: 32 822 32 888 32 1388 32 42
Cuts at: 7404 7436 1373
Size: 32 2626
Fragments arranged by size:
2626 1658 1388 888 822 700 142 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6033 6279 669
Size: 988 281 4095 246 3079
Fragments arranged by size:
4095 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3811 4850 748
Size: 3063 1039 4587
Fragments arranged by size:
4587 3063 1039
HpaI GTT'AAC
Cuts at: 6279 6279
Size: 8689
KasI G'GCGC_C
Cuts at: 4980 4980
Size: 8689
KpnI G_GTAC'C
Cuts at: 2270 5079 6014 2270
Size: 2809 935 4945
Fragments arranged by size:
4945 2809 935
MfeI C'AATT_G
Cuts at: 6288 6288
Size: 8689
MluI A'CGCG_T
Cuts at: 8565 8565
Size: 8689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2825 3841 5214 5331 5952 7803 7987 1305
Size: 1520 1016 1373 117 621 1851 184 2007
Fragments arranged by size:
2007 1851 1520 1373 1016 621 184 117
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6867
Fragments arranged by size:
6867 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3839 6647 7006 7165 518
Size: 648 2673 2808 359 159 2042
Fragments arranged by size:
2808 2673 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 4477 4756 4837 5710 6105 6782 7930
Size: 424 2447 279 81 873 395 677 1148
Cuts at: 7930 8175 1606
Size: 245 2120
Fragments arranged by size:
2447 2120 1148 873 677 424 395 279
245 81
NarI GG'CG_CC
Cuts at: 4981 4981
Size: 8689
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 7180
Fragments arranged by size:
7180 957 552
NdeI CA'TA_TG
Cuts at: 387 3888 387
Size: 3501 5188
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8689
NotI GC'GGCC_GC
Cuts at: 6136 6136
Size: 8689
NsiI A_TGCA'T
Cuts at: 2490 3675 5935 2490
Size: 1185 2260 5244
Fragments arranged by size:
5244 2260 1185
NspI r_CATG'y
Cuts at: 1132 1229 2581 4899 6047 7592 1132
Size: 97 1352 2318 1148 1545 2229
Fragments arranged by size:
2318 2229 1545 1352 1148 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8689
PciI A'CATG_T
Cuts at: 7588 7588
Size: 8689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4148 1108
Size: 3040 5649
PfoI T'CCnGG_A
Cuts at: 2235 2235
Size: 8689
PmeI GTTT'AAAC
Cuts at: 5993 5993
Size: 8689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6758 6790 8271 8303 6758
Size: 32 1481 32 7144
Fragments arranged by size:
7144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3725 5345 3725
Size: 1620 7069
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8689
PsiI TTA'TAA
Cuts at: 6259 6259
Size: 8689
PspOMI G'GGCC_C
Cuts at: 4635 4635
Size: 8689
PstI C_TGCA'G
Cuts at: 830 2818 3963 6041 830
Size: 1988 1145 2078 3478
Fragments arranged by size:
3478 2078 1988 1145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8689
PvuII CAG'CTG
Cuts at: 4837 5710 4837
Size: 873 7816
SacI G_AGCT'C
Cuts at: 2074 5173 2074
Size: 3099 5590
SalI G'TCGA_C
Cuts at: 1936 6031 1936
Size: 4095 4594
SapI GCTCTTCn'nnn_
Cuts at: 3039 4338 4965 5013 3039
Size: 1299 627 48 6715
Fragments arranged by size:
6715 1299 627 48
SbfI CC_TGCA'GG
Cuts at: 6041 6041
Size: 8689
ScaI AGT'ACT
Cuts at: 6937 8649 6937
Size: 1712 6977
SexAI A'CCwGG_T
Cuts at: 3223 3223
Size: 8689
SfcI C'TryA_G
Cuts at: 826 1045 2814 3959 4188 5397 6037 7172 7853
Size: 219 1769 1145 229 1209 640 1135 681
Cuts at: 7853 8044 826
Size: 191 1471
Fragments arranged by size:
1769 1471 1209 1145 1135 681 640 229
219 191
SfoI GGC'GCC
Cuts at: 4982 4982
Size: 8689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8689
SmaI CCC'GGG
Cuts at: 1872 4640 6016 1872
Size: 2768 1376 4545
Fragments arranged by size:
4545 2768 1376
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5660 6793 7694 7956 820
Size: 197 926 3717 1133 901 262 1553
Fragments arranged by size:
3717 1553 1133 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5986 6480 493
Size: 5493 494 2702
Fragments arranged by size:
5493 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8689
SphI G_CATG'C
Cuts at: 1132 6047 1132
Size: 4915 3774
SspI AAT'ATT
Cuts at: 5 52 6613 5
Size: 47 6561 2081
Fragments arranged by size:
6561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8689
StyI C'CwwG_G
Cuts at: 513 1065 2022 2617 5348 5637 6128 513
Size: 552 957 595 2731 289 491 3074
Fragments arranged by size:
3074 2731 957 595 552 491 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5945 6716 6733 6886 7071 7516 325
Size: 1255 4365 771 17 153 185 445 1498
Fragments arranged by size:
4365 1498 1255 771 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 3022 5795
Size: 274 80 33 51 1443 603 442 2773
Cuts at: 5795 6935 8647 96
Size: 1140 1712 138
Fragments arranged by size:
2773 1712 1443 1140 603 442 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2343 2346 2375 2378 5762 5794 8649 8681 2343
Size: 3 29 3 3384 32 2855 32 2351
Fragments arranged by size:
3384 2855 2351 32 32 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 1316 5267 1316
Size: 3951 4738
XbaI T'CTAG_A
Cuts at: 6025 6025
Size: 8689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4918 4918
Size: 8689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8689
XmaI C'CCGG_G
Cuts at: 1870 4638 6014 1870
Size: 2768 1376 4545
Fragments arranged by size:
4545 2768 1376
XmnI GAAnn'nnTTC
Cuts at: 2247 2296 2386 3756 4260 4389 6818 2247
Size: 49 90 1370 504 129 2429 4118
Fragments arranged by size:
4118 2429 1370 504 129 90 49
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2843 6497 276
Size: 53 83 186 936 1309 3654 2468
Fragments arranged by size:
3654 2468 1309 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI Bpu10I BsiWI BstZ17I
DraIII FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI