(Circular) (Six-base) MAPSORT of: pFN21ASDA0642.seq Check: 5923 from: 1 to: 8689 pFN21ASDA0642 8689 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3966 3966 Size: 8689 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2845 6499 278 Size: 53 83 186 936 1309 3654 2468 Fragments arranged by size: 3654 2468 1309 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3337 6032 1937 Size: 1400 2695 4594 Fragments arranged by size: 4594 2695 1400 Acc65I G'GTAC_C Cuts at: 2266 5075 6010 2266 Size: 2809 935 4945 Fragments arranged by size: 4945 2809 935 AclI AA'CG_TT Cuts at: 1563 6816 7189 1563 Size: 5253 373 3063 Fragments arranged by size: 5253 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2184 2988 3219 3330 3393 4151 4247 4994 Size: 552 804 231 111 63 758 96 747 Cuts at: 4994 5055 5471 6752 8136 8404 1632 Size: 61 416 1281 1384 268 1917 Fragments arranged by size: 1917 1384 1281 804 758 747 552 416 268 231 111 96 63 61 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8492 AflIII A'CryG_T Cuts at: 1203 7588 8565 1203 Size: 6385 977 1327 Fragments arranged by size: 6385 1327 977 AgeI A'CCGG_T Cuts at: 7520 7520 Size: 8689 AhdI GACnn_n'nnGTC Cuts at: 3244 7418 3244 Size: 4174 4515 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2501 4506 1184 Size: 1317 2005 5367 Fragments arranged by size: 5367 2005 1317 AlwNI CAG_nnn'CTG Cuts at: 2044 3394 3530 3958 4148 4247 5710 6432 8004 Size: 1350 136 428 190 99 1463 722 1572 Cuts at: 8004 2044 Size: 2729 Fragments arranged by size: 2729 1572 1463 1350 722 428 190 136 99 ApaI G_GGCC'C Cuts at: 4639 4639 Size: 8689 ApaLI G'TGCA_C Cuts at: 3600 6746 7902 3600 Size: 3146 1156 4387 Fragments arranged by size: 4387 3146 1156 ApoI r'AATT_y Cuts at: 1493 2219 2629 2672 3717 4386 5212 5353 5998 Size: 726 410 43 1045 669 826 141 645 Cuts at: 5998 6224 6376 8485 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1045 826 726 669 645 410 226 152 141 43 AseI AT'TA_AT Cuts at: 160 1032 7243 160 Size: 872 6211 1606 Fragments arranged by size: 6211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8689 AvaI C'yCGr_G Cuts at: 1870 1943 4638 6014 1870 Size: 73 2695 1376 4545 Fragments arranged by size: 4545 2695 1376 73 AvrII C'CTAG_G Cuts at: 5348 5348 Size: 8689 BamHI G'GATC_C Cuts at: 6019 6386 6019 Size: 367 8322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 4980 5075 6010 7465 Size: 325 217 627 479 2714 95 935 1455 Cuts at: 7465 618 Size: 1842 Fragments arranged by size: 2714 1842 1455 935 627 479 325 217 95 BanII G_rGCy'C Cuts at: 1391 2074 2371 4639 5173 5228 6008 1391 Size: 683 297 2268 534 55 780 4072 Fragments arranged by size: 4072 2268 780 683 534 297 55 BbeI G_GCGC'C Cuts at: 4984 4984 Size: 8689 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4023 4378 4555 928 Size: 971 2124 355 177 5062 Fragments arranged by size: 5062 2124 971 355 177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6880 6914 1903 Size: 34 4943 34 3678 Fragments arranged by size: 4943 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6581 7791 6581 Size: 1210 7479 BclI T'GATC_A Cuts at: 1548 4099 1548 Size: 2551 6138 BfrBI ATG'CAT Cuts at: 2488 3673 5933 2488 Size: 1185 2260 5244 Fragments arranged by size: 5244 2260 1185 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2062 7300 136 Size: 107 122 71 631 605 390 5238 1525 Fragments arranged by size: 5238 1525 631 605 390 122 107 71 BglII A'GATC_T Cuts at: 8684 8684 Size: 8689 BlpI GC'TnA_GC Cuts at: 6106 6106 Size: 8689 Bme1580I G_kGCm'C Cuts at: 1790 3354 3604 4639 6750 7906 1790 Size: 1564 250 1035 2111 1156 2573 Fragments arranged by size: 2573 2111 1564 1156 1035 250 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5901 6420 7378 Size: 416 53 83 399 24 4484 519 958 Cuts at: 7378 442 Size: 1753 Fragments arranged by size: 4484 1753 958 519 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8689 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3106 3106 Size: 8689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2097 2219 3018 3849 4766 5114 7349 1239 Size: 858 122 799 831 917 348 2235 2579 Fragments arranged by size: 2579 2235 917 858 831 799 348 122 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5681 6814 7679 7977 5681 Size: 1133 865 298 6393 Fragments arranged by size: 6393 1133 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2617 3551 7352 882 Size: 617 1118 934 3801 2219 Fragments arranged by size: 3801 2219 1118 934 617 BsaAI yAC'GTr Cuts at: 493 1204 5986 6480 493 Size: 711 4782 494 2702 Fragments arranged by size: 4782 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3622 8683 3622 Size: 5061 3628 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2842 4981 6496 6878 Size: 53 83 186 936 1309 2139 1515 382 Cuts at: 6878 7513 275 Size: 635 1451 Fragments arranged by size: 2139 1515 1451 1309 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4803 7122 7520 7794 7941 8352 4803 Size: 2319 398 274 147 411 5140 Fragments arranged by size: 5140 2319 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2086 2116 2343 2373 3406 Size: 30 165 30 683 30 227 30 1033 Cuts at: 3406 3436 5227 5257 1178 Size: 30 1791 30 4610 Fragments arranged by size: 4610 1791 1033 683 227 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2094 2358 2361 2364 2484 3098 3329 3414 Size: 908 264 3 3 120 614 231 85 Cuts at: 3414 3873 4576 4634 4685 4709 1186 Size: 459 703 58 51 24 5166 Fragments arranged by size: 5166 908 703 614 459 264 231 120 85 58 51 24 3 3 BseYI C'CCAG_C Cuts at: 2502 2599 3353 4035 4656 7892 8388 2502 Size: 97 754 682 621 3236 496 2803 Fragments arranged by size: 3236 2803 754 682 621 496 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5180 5879 1163 Size: 573 3444 699 3973 Fragments arranged by size: 3973 3444 699 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6139 6900 7928 1540 Size: 275 186 4138 761 1028 2301 Fragments arranged by size: 4138 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2074 3092 3502 3604 3642 5173 6750 6835 7906 Size: 1018 410 102 38 1531 1577 85 1071 Cuts at: 7906 2074 Size: 2857 Fragments arranged by size: 2857 1577 1531 1071 1018 410 102 85 38 BsmI GAATG_Cn' Cuts at: 3002 5185 6200 6293 3002 Size: 2183 1015 93 5398 Fragments arranged by size: 5398 2183 1015 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5960 5960 Size: 8689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2371 3092 3354 3502 3604 3642 Size: 399 284 297 721 262 148 102 38 Cuts at: 3642 4639 5173 5228 6008 6750 6835 7906 1391 Size: 997 534 55 780 742 85 1071 2174 Fragments arranged by size: 2174 1071 997 780 742 721 534 399 297 284 262 148 102 85 55 38 BspEI T'CCGG_A Cuts at: 4803 8352 4803 Size: 3549 5140 BspHI T'CATG_A Cuts at: 3834 6578 8308 3834 Size: 2744 1730 4215 Fragments arranged by size: 4215 2744 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3966 5202 6044 844 Size: 3122 1236 842 3489 Fragments arranged by size: 3489 3122 1236 842 BsrBI CCG'CTC Cuts at: 1394 1823 6576 1394 Size: 429 4753 3507 Fragments arranged by size: 4753 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2117 3687 7184 7358 65 Size: 1183 869 1570 3497 174 1396 Fragments arranged by size: 3497 1570 1396 1183 869 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4677 5412 7333 7520 1700 Size: 110 2867 735 1921 187 2869 Fragments arranged by size: 2869 2867 1921 735 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8689 BssSI C'ACGA_G Cuts at: 6749 7761 6749 Size: 1012 7677 BstAPI GCAn_nnn'nTGC Cuts at: 794 3958 5710 794 Size: 3164 1752 3773 Fragments arranged by size: 3773 3164 1752 BstBI TT'CG_AA Cuts at: 2007 7493 2007 Size: 5486 3203 BstEII G'GTnAC_C Cuts at: 1184 2888 1184 Size: 1704 6985 BstXI CCAn_nnnn'nTGG Cuts at: 3824 3824 Size: 8689 BstYI r'GATC_y Cuts at: 1972 3703 4216 5097 5902 6019 6386 6771 6788 Size: 1731 513 881 805 117 367 385 17 Cuts at: 6788 8240 8326 8338 8684 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1731 1452 881 805 513 385 367 346 117 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3708 3708 Size: 8689 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 7180 Fragments arranged by size: 7180 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7950 Fragments arranged by size: 7950 683 56 BtsI GCAGTG_nn' Cuts at: 2917 3520 5337 5501 6205 6999 7019 2917 Size: 603 1817 164 704 794 20 4587 Fragments arranged by size: 4587 1817 794 704 603 164 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8654 DraI TTT'AAA Cuts at: 5993 5993 Size: 8689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2793 7696 809 Size: 507 1477 4903 1802 Fragments arranged by size: 4903 1802 1477 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3473 6136 7025 Size: 54 1425 50 275 18 1643 2663 889 Cuts at: 7025 8654 8 Size: 1629 43 Fragments arranged by size: 2663 1643 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6136 1537 Size: 275 4324 4090 Fragments arranged by size: 4324 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3039 3111 3306 3432 3788 4303 4332 4338 Size: 1686 72 195 126 356 515 29 6 Cuts at: 4338 4452 4506 4551 4561 4566 4611 4686 4727 Size: 114 54 45 10 5 45 75 41 Cuts at: 4727 4965 5013 5094 5103 6619 1353 Size: 238 48 81 9 1516 3423 Fragments arranged by size: 3423 1686 1516 515 356 238 195 126 114 81 75 72 54 48 45 45 41 29 10 9 6 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4600 7276 7650 7796 387 Size: 1064 138 3011 2676 374 146 1280 Fragments arranged by size: 3011 2676 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2072 5171 2072 Size: 3099 5590 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2184 2219 2988 3018 3219 3330 Size: 393 465 87 35 769 30 201 111 Cuts at: 3330 3393 3849 4151 4247 4766 4994 5055 5114 Size: 63 456 302 96 519 228 61 59 Cuts at: 5114 5471 6752 7349 8136 8404 1239 Size: 357 1281 597 787 268 1524 Fragments arranged by size: 1524 1281 787 769 597 519 465 456 393 357 302 268 228 201 111 96 87 63 61 59 35 30 EcoNI CCTnn'n_nnAGG Cuts at: 2237 2237 Size: 8689 EcoO109I rG'GnC_Cy Cuts at: 3725 4244 5345 5610 3725 Size: 519 1101 265 6804 Fragments arranged by size: 6804 1101 519 265 EcoRI G'AATT_C Cuts at: 5212 5353 5998 6376 5212 Size: 141 645 378 7525 Fragments arranged by size: 7525 645 378 141 EcoRV GAT'ATC Cuts at: 3832 3832 Size: 8689 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4454 4559 4454 Size: 105 8584 FspI TGC'GCA Cuts at: 7195 7195 Size: 8689 HaeII r_GCGC'y Cuts at: 1678 4984 5668 7836 1678 Size: 3306 684 2168 2531 Fragments arranged by size: 3306 2531 2168 684 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3230 3262 3404 3436 4136 Size: 32 135 32 1658 32 142 32 700 Cuts at: 4136 4168 4990 5022 5910 5942 7330 7362 7404 Size: 32 822 32 888 32 1388 32 42 Cuts at: 7404 7436 1373 Size: 32 2626 Fragments arranged by size: 2626 1658 1388 888 822 700 142 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6033 6279 669 Size: 988 281 4095 246 3079 Fragments arranged by size: 4095 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3811 4850 748 Size: 3063 1039 4587 Fragments arranged by size: 4587 3063 1039 HpaI GTT'AAC Cuts at: 6279 6279 Size: 8689 KasI G'GCGC_C Cuts at: 4980 4980 Size: 8689 KpnI G_GTAC'C Cuts at: 2270 5079 6014 2270 Size: 2809 935 4945 Fragments arranged by size: 4945 2809 935 MfeI C'AATT_G Cuts at: 6288 6288 Size: 8689 MluI A'CGCG_T Cuts at: 8565 8565 Size: 8689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2825 3841 5214 5331 5952 7803 7987 1305 Size: 1520 1016 1373 117 621 1851 184 2007 Fragments arranged by size: 2007 1851 1520 1373 1016 621 184 117 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6867 Fragments arranged by size: 6867 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3839 6647 7006 7165 518 Size: 648 2673 2808 359 159 2042 Fragments arranged by size: 2808 2673 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 4477 4756 4837 5710 6105 6782 7930 Size: 424 2447 279 81 873 395 677 1148 Cuts at: 7930 8175 1606 Size: 245 2120 Fragments arranged by size: 2447 2120 1148 873 677 424 395 279 245 81 NarI GG'CG_CC Cuts at: 4981 4981 Size: 8689 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 7180 Fragments arranged by size: 7180 957 552 NdeI CA'TA_TG Cuts at: 387 3888 387 Size: 3501 5188 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8689 NotI GC'GGCC_GC Cuts at: 6136 6136 Size: 8689 NsiI A_TGCA'T Cuts at: 2490 3675 5935 2490 Size: 1185 2260 5244 Fragments arranged by size: 5244 2260 1185 NspI r_CATG'y Cuts at: 1132 1229 2581 4899 6047 7592 1132 Size: 97 1352 2318 1148 1545 2229 Fragments arranged by size: 2318 2229 1545 1352 1148 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8689 PciI A'CATG_T Cuts at: 7588 7588 Size: 8689 PflMI CCAn_nnn'nTGG Cuts at: 1108 4148 1108 Size: 3040 5649 PfoI T'CCnGG_A Cuts at: 2235 2235 Size: 8689 PmeI GTTT'AAAC Cuts at: 5993 5993 Size: 8689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6758 6790 8271 8303 6758 Size: 32 1481 32 7144 Fragments arranged by size: 7144 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3725 5345 3725 Size: 1620 7069 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8689 PsiI TTA'TAA Cuts at: 6259 6259 Size: 8689 PspOMI G'GGCC_C Cuts at: 4635 4635 Size: 8689 PstI C_TGCA'G Cuts at: 830 2818 3963 6041 830 Size: 1988 1145 2078 3478 Fragments arranged by size: 3478 2078 1988 1145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8689 PvuII CAG'CTG Cuts at: 4837 5710 4837 Size: 873 7816 SacI G_AGCT'C Cuts at: 2074 5173 2074 Size: 3099 5590 SalI G'TCGA_C Cuts at: 1936 6031 1936 Size: 4095 4594 SapI GCTCTTCn'nnn_ Cuts at: 3039 4338 4965 5013 3039 Size: 1299 627 48 6715 Fragments arranged by size: 6715 1299 627 48 SbfI CC_TGCA'GG Cuts at: 6041 6041 Size: 8689 ScaI AGT'ACT Cuts at: 6937 8649 6937 Size: 1712 6977 SexAI A'CCwGG_T Cuts at: 3223 3223 Size: 8689 SfcI C'TryA_G Cuts at: 826 1045 2814 3959 4188 5397 6037 7172 7853 Size: 219 1769 1145 229 1209 640 1135 681 Cuts at: 7853 8044 826 Size: 191 1471 Fragments arranged by size: 1769 1471 1209 1145 1135 681 640 229 219 191 SfoI GGC'GCC Cuts at: 4982 4982 Size: 8689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8689 SmaI CCC'GGG Cuts at: 1872 4640 6016 1872 Size: 2768 1376 4545 Fragments arranged by size: 4545 2768 1376 SmlI C'TyrA_G Cuts at: 820 1017 1943 5660 6793 7694 7956 820 Size: 197 926 3717 1133 901 262 1553 Fragments arranged by size: 3717 1553 1133 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5986 6480 493 Size: 5493 494 2702 Fragments arranged by size: 5493 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8689 SphI G_CATG'C Cuts at: 1132 6047 1132 Size: 4915 3774 SspI AAT'ATT Cuts at: 5 52 6613 5 Size: 47 6561 2081 Fragments arranged by size: 6561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8689 StyI C'CwwG_G Cuts at: 513 1065 2022 2617 5348 5637 6128 513 Size: 552 957 595 2731 289 491 3074 Fragments arranged by size: 3074 2731 957 595 552 491 289 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5945 6716 6733 6886 7071 7516 325 Size: 1255 4365 771 17 153 185 445 1498 Fragments arranged by size: 4365 1498 1255 771 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2580 3022 5795 Size: 274 80 33 51 1443 603 442 2773 Cuts at: 5795 6935 8647 96 Size: 1140 1712 138 Fragments arranged by size: 2773 1712 1443 1140 603 442 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2343 2346 2375 2378 5762 5794 8649 8681 2343 Size: 3 29 3 3384 32 2855 32 2351 Fragments arranged by size: 3384 2855 2351 32 32 29 3 3 Tth111I GACn'n_nGTC Cuts at: 1316 5267 1316 Size: 3951 4738 XbaI T'CTAG_A Cuts at: 6025 6025 Size: 8689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4918 4918 Size: 8689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8689 XmaI C'CCGG_G Cuts at: 1870 4638 6014 1870 Size: 2768 1376 4545 Fragments arranged by size: 4545 2768 1376 XmnI GAAnn'nnTTC Cuts at: 2247 2296 2386 3756 4260 4389 6818 2247 Size: 49 90 1370 504 129 2429 4118 Fragments arranged by size: 4118 2429 1370 504 129 90 49 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2843 6497 276 Size: 53 83 186 936 1309 3654 2468 Fragments arranged by size: 3654 2468 1309 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI Bpu10I BsiWI BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI