Restriction map for
pFN21ASDA0640
(Circular) (Six-base) MAPSORT of: pFN21ASDA0640.seq Check: 8843 from: 1 to: 6484
pFN21ASDA0640 6484 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 1937
Size: 1890 4594
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 2173 2783 2871 3137 3155 3353 3515
Size: 539 2 610 88 266 18 198 162
Cuts at: 3515 4547 5931 6199 1632
Size: 1032 1384 268 1917
Fragments arranged by size:
1917 1384 1032 610 539 268 266 198
162 88 18 2
AflII C'TTAA_G
Cuts at: 820 1017 2450 820
Size: 197 1433 4854
Fragments arranged by size:
4854 1433 197
AflIII A'CryG_T
Cuts at: 1203 3294 5383 6360 1203
Size: 2091 2089 977 1327
Fragments arranged by size:
2091 2089 1327 977
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 5213 5213
Size: 6484
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6484
AlwNI CAG_nnn'CTG
Cuts at: 3085 3508 4227 5799 3085
Size: 423 719 1572 3770
Fragments arranged by size:
3770 1572 719 423
ApaLI G'TGCA_C
Cuts at: 2140 4541 5697 2140
Size: 2401 1156 2927
Fragments arranged by size:
2927 2401 1156
ApoI r'AATT_y
Cuts at: 1493 2288 3793 4019 4171 6280 1493
Size: 795 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 795 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2417 2622 3653 5038 160
Size: 872 1385 205 1031 1385 1606
Fragments arranged by size:
1606 1385 1385 1031 872 205
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 3809 1870
Size: 73 1866 4545
Fragments arranged by size:
4545 1866 73
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3805 5260 618
Size: 325 217 627 2018 1455 1842
Fragments arranged by size:
2018 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3803 1391
Size: 2412 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3297 3387 928
Size: 971 1398 90 4025
Fragments arranged by size:
4025 1398 971 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4675 4709 1903
Size: 34 2738 34 3678
Fragments arranged by size:
3678 2738 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2421 4376 5586 2421
Size: 1955 1210 3319
Fragments arranged by size:
3319 1955 1210
BclI T'GATC_A
Cuts at: 1548 3693 1548
Size: 2145 4339
BfrBI ATG'CAT
Cuts at: 2366 2366
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5095 136
Size: 107 122 71 631 605 3423 1525
Fragments arranged by size:
3423 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 3901 3901
Size: 6484
Bme1580I G_kGCm'C
Cuts at: 1790 2144 4545 5701 1790
Size: 354 2401 1156 2573
Fragments arranged by size:
2573 2401 1156 354
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6484
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2162 3712 4215
Size: 416 53 83 399 24 745 1550 503
Cuts at: 4215 5173 442
Size: 958 1753
Fragments arranged by size:
1753 1550 958 745 503 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2801 2801
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3038 3053 3107 3128 3551 3614 5144 1239
Size: 1799 15 54 21 423 63 1530 2579
Fragments arranged by size:
2579 1799 1530 423 63 54 21 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2102 3144 3560 3584 4609 5474 5772 2102
Size: 1042 416 24 1025 865 298 2814
Fragments arranged by size:
2814 1042 1025 865 416 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2107 5147 882
Size: 617 608 3040 2219
Fragments arranged by size:
3040 2219 617 608
BsaAI yAC'GTr
Cuts at: 493 1204 3781 4275 493
Size: 711 2577 494 2702
Fragments arranged by size:
2702 2577 711 494
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4291 4673 5308 275
Size: 53 83 186 936 2758 382 635 1451
Fragments arranged by size:
2758 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3000 4917 5315 5589 5736 6147 3000
Size: 1917 398 274 147 411 3337
Fragments arranged by size:
3337 1917 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3377 3407 3527 3557 1178
Size: 30 165 30 1974 30 120 30 4105
Fragments arranged by size:
4105 1974 165 120 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2490 3548 1186
Size: 868 436 1058 4122
Fragments arranged by size:
4122 1058 868 436
BseYI C'CCAG_C
Cuts at: 2112 2950 5687 6183 2112
Size: 838 2737 496 2413
Fragments arranged by size:
2737 2413 838 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3147 1163
Size: 573 1411 4500
Fragments arranged by size:
4500 1411 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3934 4695 5723 1540
Size: 275 186 1933 761 1028 2301
Fragments arranged by size:
2301 1933 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2144 2187 4545 4630 5701 2144
Size: 43 2358 85 1071 2927
Fragments arranged by size:
2927 2358 1071 85 43
BsmI GAATG_Cn'
Cuts at: 3995 4088 3995
Size: 93 6391
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2187 3803 4545 4630 5701 1391
Size: 399 354 43 1616 742 85 1071 2174
Fragments arranged by size:
2174 1616 1071 742 399 354 85 43
BspEI T'CCGG_A
Cuts at: 3000 6147 3000
Size: 3147 3337
BspHI T'CATG_A
Cuts at: 2161 3643 4373 6103 2161
Size: 1482 730 1730 2542
Fragments arranged by size:
2542 1730 1482 730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2454 3718 3839 844
Size: 1610 1264 121 3489
Fragments arranged by size:
3489 1610 1264 121
BsrBI CCG'CTC
Cuts at: 1394 1823 4371 1394
Size: 429 2548 3507
Fragments arranged by size:
3507 2548 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4979 5153 65
Size: 1183 3731 174 1396
Fragments arranged by size:
3731 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2598 5128 5315 1700
Size: 110 788 2530 187 2869
Fragments arranged by size:
2869 2530 788 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6484
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6484
BssSI C'ACGA_G
Cuts at: 3123 4544 5556 3123
Size: 1421 1012 4051
Fragments arranged by size:
4051 1421 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3125 794
Size: 2331 4153
BstBI TT'CG_AA
Cuts at: 2007 5288 2007
Size: 3281 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstXI CCAn_nnnn'nTGG
Cuts at: 3382 3382
Size: 6484
BstYI r'GATC_y
Cuts at: 1972 2758 3814 4181 4566 4583 6035 6121 6133
Size: 786 1056 367 385 17 1452 86 12
Cuts at: 6133 6479 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1056 786 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3519 3771 513
Size: 552 957 1497 252 3226
Fragments arranged by size:
3226 1497 957 552 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5745
Fragments arranged by size:
5745 683 56
BtsI GCAGTG_nn'
Cuts at: 4000 4794 4814 4000
Size: 794 20 5670
Fragments arranged by size:
5670 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 2241 2371 3788 2241
Size: 130 1417 4937
Fragments arranged by size:
4937 1417 130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5491 809
Size: 507 4175 1802
Fragments arranged by size:
4175 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3931 4820 6449
Size: 54 1425 50 275 18 2101 889 1629
Cuts at: 6449 8
Size: 43
Fragments arranged by size:
2101 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3931 1537
Size: 275 2119 4090
Fragments arranged by size:
4090 2119 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2175 2427 3129 3345 3384 3732 4414 1353
Size: 822 252 702 216 39 348 682 3423
Fragments arranged by size:
3423 822 702 682 348 252 216 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3476 5071 5445 5591 387
Size: 1064 138 1887 1595 374 146 1280
Fragments arranged by size:
1887 1595 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2173 2783 2871 3038 3053 3107
Size: 393 539 2 610 88 167 15 54
Cuts at: 3107 3128 3137 3155 3353 3515 3551 3614 4547
Size: 21 9 18 198 162 36 63 933
Cuts at: 4547 5144 5931 6199 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 933 787 610 597 539 393 268
198 167 162 88 63 54 36 21
18 15 9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 3512 3512
Size: 6484
EcoO109I rG'GnC_Cy
Cuts at: 2122 3505 3707 2122
Size: 1383 202 4899
Fragments arranged by size:
4899 1383 202
EcoRI G'AATT_C
Cuts at: 2288 3793 4171 2288
Size: 1505 378 4601
Fragments arranged by size:
4601 1505 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3185 3442 3185
Size: 257 6227
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 5631 1678
Size: 3953 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2743 2775 3377 3409 5125
Size: 32 135 32 1171 32 602 32 1716
Cuts at: 5125 5157 5199 5231 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1716 1171 602 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3828 4074 669
Size: 988 281 1890 246 3079
Fragments arranged by size:
3079 1890 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2456 3206 3365 748
Size: 1708 750 159 3867
Fragments arranged by size:
3867 1708 750 159
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 2285 2969 3363 3456 5598 5782 1305
Size: 936 44 684 394 93 2142 184 2007
Fragments arranged by size:
2142 2007 936 684 394 184 93 44
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4662
Fragments arranged by size:
4662 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4442 4801 4960 518
Size: 648 3276 359 159 2042
Fragments arranged by size:
3276 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2094 3013 3529 3900 4577 5725 5970 1606
Size: 488 919 516 371 677 1148 245 2120
Fragments arranged by size:
2120 1148 919 677 516 488 371 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 3519 513
Size: 552 957 1497 3478
Fragments arranged by size:
3478 1497 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NsiI A_TGCA'T
Cuts at: 2368 2368
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 2233 3298 3842 5387 1132
Size: 97 1004 1065 544 1545 2229
Fragments arranged by size:
2229 1545 1065 1004 544 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6484
PciI A'CATG_T
Cuts at: 3294 5383 3294
Size: 2089 4395
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3621 1108
Size: 2513 3971
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 3707 2122
Size: 1585 4899
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 2508 4054 2508
Size: 1546 4938
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3252 3284 3252
Size: 32 6452
PstI C_TGCA'G
Cuts at: 830 3312 3513 3715 3836 830
Size: 2482 201 202 121 3478
Fragments arranged by size:
3478 2482 202 201 121
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 3013 3529 3013
Size: 516 5968
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SapI GCTCTTCn'nnn_
Cuts at: 2175 2175
Size: 6484
SbfI CC_TGCA'GG
Cuts at: 3513 3836 3513
Size: 323 6161
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SexAI A'CCwGG_T
Cuts at: 3670 3670
Size: 6484
SfcI C'TryA_G
Cuts at: 826 1045 3308 3509 3711 3832 4967 5648 5839
Size: 219 2263 201 202 121 1135 681 191
Cuts at: 5839 826
Size: 1471
Fragments arranged by size:
2263 1471 1135 681 219 202 201 191
121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3811 1872
Size: 1939 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 2450 3159 3539 3563 4588
Size: 197 926 174 333 709 380 24 1025
Cuts at: 4588 5489 5751 820
Size: 901 262 1553
Fragments arranged by size:
1553 1025 926 901 709 380 333 262
197 174 24
SnaBI TAC'GTA
Cuts at: 493 3781 4275 493
Size: 3288 494 2702
Fragments arranged by size:
3288 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3842 1132
Size: 2710 3774
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6484
StyI C'CwwG_G
Cuts at: 513 1065 2022 3519 3923 513
Size: 552 957 1497 404 3074
Fragments arranged by size:
3074 1497 957 552 404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4511 4528 4681 4866 5311 325
Size: 1255 2931 17 153 185 445 1498
Fragments arranged by size:
2931 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2719 4730 6442
Size: 274 80 33 51 1443 742 2011 1712
Cuts at: 6442 96
Size: 138
Fragments arranged by size:
2011 1712 1443 742 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2299 2323 2331 2355 6444 6476 2299
Size: 24 8 24 4089 32 2307
Fragments arranged by size:
4089 2307 32 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 1316 2608 1316
Size: 1292 5192
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3526 3526
Size: 6484
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 3809 1870
Size: 1939 4545
XmnI GAAnn'nnTTC
Cuts at: 4613 4613
Size: 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI