Restriction map for
pFN21ASDA0638
(Circular) (Six-base) MAPSORT of: pFN21ASDA0638.seq Check: 1112 from: 1 to: 8860
pFN21ASDA0638 8860 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5313 5973 5313
Size: 660 8200
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6670 278
Size: 53 83 186 936 5134 2468
Fragments arranged by size:
5134 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4785 6203 1937
Size: 2848 1418 4594
Fragments arranged by size:
4594 2848 1418
Acc65I G'GTAC_C
Cuts at: 2875 4800 6181 2875
Size: 1925 1381 5554
Fragments arranged by size:
5554 1925 1381
AclI AA'CG_TT
Cuts at: 1563 6987 7360 1563
Size: 5424 373 3063
Fragments arranged by size:
5424 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2451 3607 5207 5747 5936 6923 8307 8575
Size: 819 1156 1600 540 189 987 1384 268
Cuts at: 8575 1632
Size: 1917
Fragments arranged by size:
1917 1600 1384 1156 987 819 540 268
189
AfeI AGC'GCT
Cuts at: 4064 5044 5849 4064
Size: 980 805 7075
Fragments arranged by size:
7075 980 805
AflII C'TTAA_G
Cuts at: 820 1017 3400 820
Size: 197 2383 6280
Fragments arranged by size:
6280 2383 197
AflIII A'CryG_T
Cuts at: 1203 5430 7759 8736 1203
Size: 4227 2329 977 1327
Fragments arranged by size:
4227 2329 1327 977
AgeI A'CCGG_T
Cuts at: 7691 7691
Size: 8860
AhdI GACnn_n'nnGTC
Cuts at: 5431 7589 5431
Size: 2158 6702
AleI CACnn'nnGTG
Cuts at: 1166 6005 1166
Size: 4839 4021
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4146 1184
Size: 2962 5898
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4433 4465 4433
Size: 32 8828
AlwNI CAG_nnn'CTG
Cuts at: 3035 3128 3188 3862 4192 4243 4510 4660 4810
Size: 93 60 674 330 51 267 150 150
Cuts at: 4810 5560 5632 6128 6603 8175 3035
Size: 750 72 496 475 1572 3720
Fragments arranged by size:
3720 1572 750 674 496 475 330 267
150 150 93 72 60 51
ApaI G_GGCC'C
Cuts at: 2255 2484 5261 2255
Size: 229 2777 5854
Fragments arranged by size:
5854 2777 229
ApaLI G'TGCA_C
Cuts at: 6917 8073 6917
Size: 1156 7704
ApoI r'AATT_y
Cuts at: 1493 6169 6395 6547 8656 1493
Size: 4676 226 152 2109 1697
Fragments arranged by size:
4676 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7414 160
Size: 872 6382 1606
Fragments arranged by size:
6382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8860
AvaI C'yCGr_G
Cuts at: 1870 1943 4257 4632 5263 6185 1870
Size: 73 2314 375 631 922 4545
Fragments arranged by size:
4545 2314 922 631 375 73
BamHI G'GATC_C
Cuts at: 6190 6557 6190
Size: 367 8493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2297 2741 2875 3604 3727
Size: 325 217 627 510 444 134 729 123
Cuts at: 3727 4800 6181 7636 618
Size: 1073 1381 1455 1842
Fragments arranged by size:
1842 1455 1381 1073 729 627 510 444
325 217 134 123
BanII G_rGCy'C
Cuts at: 1391 2255 2346 2476 2484 2597 2759 3071 3427
Size: 864 91 130 8 113 162 312 356
Cuts at: 3427 3578 3641 3659 4186 4603 5261 5372 6020
Size: 151 63 18 527 417 658 111 648
Cuts at: 6020 6179 1391
Size: 159 4072
Fragments arranged by size:
4072 864 658 648 527 417 356 312
162 159 151 130 113 111 91 63
18 8
BbeI G_GCGC'C
Cuts at: 2745 2745
Size: 8860
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4742 5735 5851 928
Size: 971 2843 993 116 3937
Fragments arranged by size:
3937 2843 993 971 116
BbvCI CC'TCA_GC
Cuts at: 5205 6129 5205
Size: 924 7936
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7051 7085 1903
Size: 34 5114 34 3678
Fragments arranged by size:
5114 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6752 7962 6752
Size: 1210 7650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2365 3369 3518
Size: 107 122 71 631 605 693 1004 149
Cuts at: 3518 4262 5265 5314 7471 136
Size: 744 1003 49 2157 1525
Fragments arranged by size:
2157 1525 1004 1003 744 693 631 605
149 122 107 71 49
BglII A'GATC_T
Cuts at: 8855 8855
Size: 8860
BlpI GC'TnA_GC
Cuts at: 2960 3635 4527 5003 5181 5783 6277 2960
Size: 675 892 476 178 602 494 5543
Fragments arranged by size:
5543 892 675 602 494 476 178
Bme1580I G_kGCm'C
Cuts at: 1790 2255 2300 2484 2868 3556 4200 4282 4315
Size: 465 45 184 384 688 644 82 33
Cuts at: 4315 5261 5620 6921 8077 1790
Size: 946 359 1301 1156 2573
Fragments arranged by size:
2573 1301 1156 946 688 644 465 384
359 184 82 45 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2832 3599 3675
Size: 416 53 83 399 24 1415 767 76
Cuts at: 3675 6591 7549 442
Size: 2916 958 1753
Fragments arranged by size:
2916 1753 1415 958 767 416 399 83
76 53 24
BmtI G_CTAG'C
Cuts at: 1056 4166 1056
Size: 3110 5750
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2791 3553 2791
Size: 762 8098
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2250 2670 2743 2763 2875 3165 3200
Size: 980 31 420 73 20 112 290 35
Cuts at: 3200 3234 3694 3812 3884 4133 4436 5384 5582
Size: 34 460 118 72 249 303 948 198
Cuts at: 5582 7520 1239
Size: 1938 2579
Fragments arranged by size:
2579 1938 980 948 460 420 303 290
249 198 118 112 73 72 35 34
31 20
Bpu10I CC'TnA_GC
Cuts at: 4185 4602 5205 6129 4185
Size: 417 603 924 6916
Fragments arranged by size:
6916 924 603 417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4067 4196 5855 6985 7850 8148 4067
Size: 129 1659 1130 865 298 4779
Fragments arranged by size:
4779 1659 1130 865 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3860 5105 5378 7523 882
Size: 617 2361 1245 273 2145 2219
Fragments arranged by size:
2361 2219 2145 1245 617 273
BsaAI yAC'GTr
Cuts at: 493 1204 4201 5776 6157 6651 493
Size: 711 2997 1575 381 494 2702
Fragments arranged by size:
2997 2702 1575 711 494 381
BsaBI GATnn'nnATC
Cuts at: 8854 8854
Size: 8860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2742 6667 7049 7684
Size: 53 83 186 936 1209 3925 382 635
Cuts at: 7684 275
Size: 1451
Fragments arranged by size:
3925 1451 1209 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4515 5706 7293 7691 7965 8112 8523 4515
Size: 1191 1587 398 274 147 411 4852
Fragments arranged by size:
4852 1587 1191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8635
Fragments arranged by size:
8635 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2657 4034 4103 4496 4499 4960 4963 4978
Size: 1471 1377 69 393 3 461 3 15
Cuts at: 4978 4981 4984 5345 5483 5579 5582 1186
Size: 3 3 361 138 96 3 4464
Fragments arranged by size:
4464 1471 1377 461 393 361 138 96
69 15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2990 3076 3121 3230 3504 3547 3640 3920 3932
Size: 86 45 109 274 43 93 280 12
Cuts at: 3932 4113 4192 4586 4822 4998 5251 6025 8063
Size: 181 79 394 236 176 253 774 2038
Cuts at: 8063 8559 2990
Size: 496 3291
Fragments arranged by size:
3291 2038 774 496 394 280 274 253
236 181 176 109 93 86 79 45
43 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 2314 4114 4631 5756 5994 1163
Size: 573 363 215 1800 517 1125 238 4029
Fragments arranged by size:
4029 1800 1125 573 517 363 238 215
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5866 6310 7071 8099 1540
Size: 275 186 3865 444 761 1028 2301
Fragments arranged by size:
3865 2301 1028 761 444 275 186
BsiHKAI G_wGCw'C
Cuts at: 2274 2597 2941 3136 3427 4112 4125 5343 5372
Size: 323 344 195 291 685 13 1218 29
Cuts at: 5372 5784 6921 7006 8077 2274
Size: 412 1137 85 1071 3057
Fragments arranged by size:
3057 1218 1137 1071 685 412 344 323
291 195 85 29 13
BsmI GAATG_Cn'
Cuts at: 3153 4648 6371 6464 3153
Size: 1495 1723 93 5549
Fragments arranged by size:
5549 1723 1495 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5900 5900
Size: 8860
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2255 2274 2300 2346 2476 2484 2597
Size: 399 465 19 26 46 130 8 113
Cuts at: 2597 2759 2868 2941 3071 3136 3427 3556 3578
Size: 162 109 73 130 65 291 129 22
Cuts at: 3578 3641 3659 4112 4125 4186 4200 4282 4315
Size: 63 18 453 13 61 14 82 33
Cuts at: 4315 4603 5261 5343 5372 5620 5784 6020 6179
Size: 288 658 82 29 248 164 236 159
Cuts at: 6179 6921 7006 8077 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 658 465 453 399 291
288 248 236 164 162 159 130 130
129 113 109 85 82 82 73 65
63 61 46 33 29 26 22 19
18 14 13 8
BspEI T'CCGG_A
Cuts at: 4515 5706 8523 4515
Size: 1191 2817 4852
Fragments arranged by size:
4852 2817 1191
BspHI T'CATG_A
Cuts at: 6147 6749 8479 6147
Size: 602 1730 6528
Fragments arranged by size:
6528 1730 602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2298 2619 2724 2887 4888 5313 5578 5706
Size: 1454 321 105 163 2001 425 265 128
Cuts at: 5706 5774 5973 6215 844
Size: 68 199 242 3489
Fragments arranged by size:
3489 2001 1454 425 321 265 242 199
163 128 105 68
BsrBI CCG'CTC
Cuts at: 1394 1823 3262 3745 3820 4180 4276 4309 4465
Size: 429 1439 483 75 360 96 33 156
Cuts at: 4465 4908 5533 5572 6747 1394
Size: 443 625 39 1175 3507
Fragments arranged by size:
3507 1439 1175 625 483 443 429 360
156 96 75 39 33
BsrDI GCAATG_nn'
Cuts at: 65 1248 2329 7355 7529 65
Size: 1183 1081 5026 174 1396
Fragments arranged by size:
5026 1396 1183 1081 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3363 3801 4499 5511 5865 6057 7504
Size: 110 1553 438 698 1012 354 192 1447
Cuts at: 7504 7691 1700
Size: 187 2869
Fragments arranged by size:
2869 1553 1447 1012 698 438 354 192
187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8860
BssSI C'ACGA_G
Cuts at: 3984 6920 7932 3984
Size: 2936 1012 4912
Fragments arranged by size:
4912 2936 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2328 2388 4243 5779 5796 794
Size: 1534 60 1855 1536 17 3858
Fragments arranged by size:
3858 1855 1536 1534 60 17
BstBI TT'CG_AA
Cuts at: 2007 7664 2007
Size: 5657 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8860
BstXI CCAn_nnnn'nTGG
Cuts at: 2752 2752
Size: 8860
BstYI r'GATC_y
Cuts at: 1972 3457 4593 6190 6557 6942 6959 8411 8497
Size: 1485 1136 1597 367 385 17 1452 86
Cuts at: 8497 8509 8855 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1597 1485 1452 1136 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3915 3915
Size: 8860
BtgI C'CryG_G
Cuts at: 513 1065 2022 2073 3514 5752 5800 513
Size: 552 957 51 1441 2238 48 3573
Fragments arranged by size:
3573 2238 1441 957 552 51 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4528 487
Size: 683 56 3302 4819
Fragments arranged by size:
4819 3302 683 56
BtsI GCAGTG_nn'
Cuts at: 2167 2272 2818 3262 4280 4313 4344 4802 5096
Size: 105 546 444 1018 33 31 458 294
Cuts at: 5096 5977 6376 7170 7190 2167
Size: 881 399 794 20 3837
Fragments arranged by size:
3837 1018 881 794 546 458 444 399
294 105 33 31 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8825
DraI TTT'AAA
Cuts at: 6164 6164
Size: 8860
DraIII CAC_nnn'GTG
Cuts at: 2149 2840 3593 5779 2149
Size: 691 753 2186 5230
Fragments arranged by size:
5230 2186 753 691
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7867 809
Size: 507 6551 1802
Fragments arranged by size:
6551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3099 4497 4501
Size: 54 1425 50 275 18 1269 1398 4
Cuts at: 4501 4566 4815 6307 7196 8825 8
Size: 65 249 1492 889 1629 43
Fragments arranged by size:
1629 1492 1425 1398 1269 889 275 249
65 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 6307 1537
Size: 275 4495 4090
Fragments arranged by size:
4495 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2803 4170 4861 4987 5154 5978 6790 1353
Size: 1450 1367 691 126 167 824 812 3423
Fragments arranged by size:
3423 1450 1367 824 812 691 167 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3059 4024 5590 7447 7821 7967
Size: 1064 138 1470 965 1566 1857 374 146
Cuts at: 7967 387
Size: 1280
Fragments arranged by size:
1857 1566 1470 1280 1064 965 374 146
138
EcoICRI GAG'CTC
Cuts at: 2595 3425 5370 2595
Size: 830 1945 6085
Fragments arranged by size:
6085 1945 830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2250 2451 2670 2743 2763 2875
Size: 393 587 31 201 219 73 20 112
Cuts at: 2875 3165 3200 3234 3607 3694 3812 3884 4133
Size: 290 35 34 373 87 118 72 249
Cuts at: 4133 4436 5207 5384 5582 5747 5936 6923 7520
Size: 303 771 177 198 165 189 987 597
Cuts at: 7520 8307 8575 1239
Size: 787 268 1524
Fragments arranged by size:
1524 987 787 771 597 587 393 373
303 290 268 249 219 201 198 189
177 165 118 112 87 73 72 35
34 31 20
EcoNI CCTnn'n_nnAGG
Cuts at: 5564 5564
Size: 8860
EcoO109I rG'GnC_Cy
Cuts at: 2051 2087 2251 2481 2558 2608 2855 3370 3911
Size: 36 164 230 77 50 247 515 541
Cuts at: 3911 3978 3999 4411 5258 5557 2051
Size: 67 21 412 847 299 5354
Fragments arranged by size:
5354 847 541 515 412 299 247 230
164 77 67 50 36 21
EcoRI G'AATT_C
Cuts at: 6169 6547 6169
Size: 378 8482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3637 5734 3637
Size: 2097 6763
FseI GG_CCGG'CC
Cuts at: 4503 4503
Size: 8860
FspI TGC'GCA
Cuts at: 5969 7366 5969
Size: 1397 7463
HaeII r_GCGC'y
Cuts at: 1678 2713 2745 3505 3814 3862 4066 4564 5046
Size: 1035 32 760 309 48 204 498 482
Cuts at: 5046 5122 5851 8007 1678
Size: 76 729 2156 2531
Fragments arranged by size:
2531 2156 1035 760 729 498 482 309
204 76 48 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4585 4617 5417 5449 7501
Size: 32 135 32 3013 32 800 32 2052
Cuts at: 7501 7533 7575 7607 1373
Size: 32 42 32 2626
Fragments arranged by size:
3013 2626 2052 800 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3143 6204 6450 669
Size: 988 281 1205 3061 246 3079
Fragments arranged by size:
3079 3061 1205 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4881 748
Size: 4133 4727
HpaI GTT'AAC
Cuts at: 6450 6450
Size: 8860
KasI G'GCGC_C
Cuts at: 2741 2741
Size: 8860
KpnI G_GTAC'C
Cuts at: 2879 4804 6185 2879
Size: 1925 1381 5554
Fragments arranged by size:
5554 1925 1381
MfeI C'AATT_G
Cuts at: 6459 6459
Size: 8860
MluI A'CGCG_T
Cuts at: 8736 8736
Size: 8860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4440 7974 8158 1305
Size: 3135 3534 184 2007
Fragments arranged by size:
3534 3135 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3101 4568 10
Size: 54 1425 343 1269 1467 4302
Fragments arranged by size:
4302 1467 1425 1269 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2750 4128 5346 6005 6818 7177 7336
Size: 648 1584 1378 1218 659 813 359 159
Cuts at: 7336 518
Size: 2042
Fragments arranged by size:
2042 1584 1378 1218 813 659 648 359
159
MspA1I CmG'CkG
Cuts at: 1606 2197 2872 3088 3436 3714 4243 4348 4657
Size: 591 675 216 348 278 529 105 309
Cuts at: 4657 4838 4894 4956 5167 5204 5255 5632 6276
Size: 181 56 62 211 37 51 377 644
Cuts at: 6276 6953 8101 8346 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 675 644 591 529 377
348 309 278 245 216 211 181 105
62 56 51 37
NaeI GCC'GGC
Cuts at: 3365 4501 3365
Size: 1136 7724
NarI GG'CG_CC
Cuts at: 2742 2742
Size: 8860
NcoI C'CATG_G
Cuts at: 513 1065 2022 2073 3514 5752 513
Size: 552 957 51 1441 2238 3621
Fragments arranged by size:
3621 2238 1441 957 552 51
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8860
NgoMIV G'CCGG_C
Cuts at: 3363 4499 3363
Size: 1136 7724
NheI G'CTAG_C
Cuts at: 1052 4162 1052
Size: 3110 5750
NotI GC'GGCC_GC
Cuts at: 6307 6307
Size: 8860
NspI r_CATG'y
Cuts at: 1132 1229 2386 5434 6218 7763 1132
Size: 97 1157 3048 784 1545 2229
Fragments arranged by size:
3048 2229 1545 1157 784 97
PasI CC'CwG_GG
Cuts at: 1348 2477 5017 1348
Size: 1129 2540 5191
Fragments arranged by size:
5191 2540 1129
PciI A'CATG_T
Cuts at: 5430 7759 5430
Size: 2329 6531
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2074 3128 3295 3862 4459 1108
Size: 966 1054 167 567 597 5509
Fragments arranged by size:
5509 1054 966 597 567 167
PfoI T'CCnGG_A
Cuts at: 3216 3789 3825 4572 3216
Size: 573 36 747 7504
Fragments arranged by size:
7504 747 573 36
PmeI GTTT'AAAC
Cuts at: 6164 6164
Size: 8860
PmlI CAC'GTG
Cuts at: 4201 5776 4201
Size: 1575 7285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6929 6961 8442 8474 6929
Size: 32 1481 32 7315
Fragments arranged by size:
7315 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2087 2558 2608 2855 3911 2087
Size: 471 50 247 1056 7036
Fragments arranged by size:
7036 1056 471 247 50
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8860
PsiI TTA'TAA
Cuts at: 6430 6430
Size: 8860
PspOMI G'GGCC_C
Cuts at: 2251 2480 5257 2251
Size: 229 2777 5854
Fragments arranged by size:
5854 2777 229
PstI C_TGCA'G
Cuts at: 830 2230 2506 3031 3339 4248 4290 4323 4353
Size: 1400 276 525 308 909 42 33 30
Cuts at: 4353 4843 4899 5103 5136 5607 6088 6212 830
Size: 490 56 204 33 471 481 124 3478
Fragments arranged by size:
3478 1400 909 525 490 481 471 308
276 204 124 56 42 33 33 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8860
PvuII CAG'CTG
Cuts at: 3088 4243 4348 4657 4894 5204 5632 3088
Size: 1155 105 309 237 310 428 6316
Fragments arranged by size:
6316 1155 428 310 309 237 105
SacI G_AGCT'C
Cuts at: 2597 3427 5372 2597
Size: 830 1945 6085
Fragments arranged by size:
6085 1945 830
SalI G'TCGA_C
Cuts at: 1936 6202 1936
Size: 4266 4594
SanDI GG'GwC_CC
Cuts at: 2558 2558
Size: 8860
SapI GCTCTTCn'nnn_
Cuts at: 4170 4987 5154 4170
Size: 817 167 7876
Fragments arranged by size:
7876 817 167
SbfI CC_TGCA'GG
Cuts at: 6212 6212
Size: 8860
ScaI AGT'ACT
Cuts at: 7108 8820 7108
Size: 1712 7148
SexAI A'CCwGG_T
Cuts at: 2145 2145
Size: 8860
SfcI C'TryA_G
Cuts at: 826 1045 2226 2246 2488 2502 2909 3027 3335
Size: 219 1181 20 242 14 407 118 308
Cuts at: 3335 3646 4244 4286 4319 4349 4782 4839 4895
Size: 311 598 42 33 30 433 57 56
Cuts at: 4895 5099 5132 5603 6084 6208 7343 8024 8215
Size: 204 33 471 481 124 1135 681 191
Cuts at: 8215 826
Size: 1471
Fragments arranged by size:
1471 1181 1135 681 598 481 471 433
407 311 308 242 219 204 191 124
118 57 56 42 33 33 30 20
14
SfiI GGCCn_nnn'nGGCC
Cuts at: 3369 3369
Size: 8860
SfoI GGC'GCC
Cuts at: 2743 2743
Size: 8860
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8860
SgrAI Cr'CCGG_yG
Cuts at: 1700 3801 1700
Size: 2101 6759
SmaI CCC'GGG
Cuts at: 1872 4634 6187 1872
Size: 2762 1553 4545
Fragments arranged by size:
4545 2762 1553
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3400 4082 4211 5870 6964 7865
Size: 197 926 1457 682 129 1659 1094 901
Cuts at: 7865 8127 820
Size: 262 1553
Fragments arranged by size:
1659 1553 1457 1094 926 901 682 262
197 129
SnaBI TAC'GTA
Cuts at: 493 6157 6651 493
Size: 5664 494 2702
Fragments arranged by size:
5664 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8860
SphI G_CATG'C
Cuts at: 1132 6218 1132
Size: 5086 3774
SspI AAT'ATT
Cuts at: 5 52 6784 5
Size: 47 6732 2081
Fragments arranged by size:
6732 2081 47
StuI AGG'CCT
Cuts at: 1517 2259 2986 3990 4726 1517
Size: 742 727 1004 736 5651
Fragments arranged by size:
5651 1004 742 736 727
StyI C'CwwG_G
Cuts at: 513 1065 2022 2073 2091 3514 4368 5752 6299
Size: 552 957 51 18 1423 854 1384 547
Cuts at: 6299 513
Size: 3074
Fragments arranged by size:
3074 1423 1384 957 854 552 547 51
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2851 3068 3581 3598 4052 4127 5069
Size: 1255 1271 217 513 17 454 75 942
Cuts at: 5069 6887 6904 7057 7242 7687 325
Size: 1818 17 153 185 445 1498
Fragments arranged by size:
1818 1498 1271 1255 942 513 454 445
217 185 153 75 17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5952 6063 7106
Size: 274 80 33 51 1443 3975 111 1043
Cuts at: 7106 8818 96
Size: 1712 138
Fragments arranged by size:
3975 1712 1443 1043 274 138 111 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2847 2879 3139 3171 3442 3474 5136 5168 8820
Size: 32 260 32 271 32 1662 32 3652
Cuts at: 8820 8852 2847
Size: 32 2855
Fragments arranged by size:
3652 2855 1662 271 260 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8860
XbaI T'CTAG_A
Cuts at: 6196 6196
Size: 8860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2051 2899 3955 2051
Size: 848 1056 6956
Fragments arranged by size:
6956 1056 848
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8860
XmaI C'CCGG_G
Cuts at: 1870 4632 6185 1870
Size: 2762 1553 4545
Fragments arranged by size:
4545 2762 1553
XmnI GAAnn'nnTTC
Cuts at: 3307 6989 3307
Size: 3682 5178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6668 276
Size: 53 83 186 936 5134 2468
Fragments arranged by size:
5134 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FspAI
NruI NsiI PacI PspXI PsrI RsrII SacII SrfI
SwaI