Restriction map for
pFN21ASDA0637
(Circular) (Six-base) MAPSORT of: pFN21ASDA0637.seq Check: 5966 from: 1 to: 8242
pFN21ASDA0637 8242 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4333 4333
Size: 8242
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4686 6052 278
Size: 53 83 186 936 3150 1366 2468
Fragments arranged by size:
3150 2468 1366 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4050 5585 1937
Size: 2113 1535 4594
Fragments arranged by size:
4594 2113 1535
Acc65I G'GTAC_C
Cuts at: 3809 5563 3809
Size: 1754 6488
AclI AA'CG_TT
Cuts at: 1563 4056 6369 6742 1563
Size: 2493 2313 373 3063
Fragments arranged by size:
3063 2493 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2739 3413 4409 4489 4997 5286 5360 5397
Size: 1107 674 996 80 508 289 74 37
Cuts at: 5397 6305 7689 7957 1632
Size: 908 1384 268 1917
Fragments arranged by size:
1917 1384 1107 996 908 674 508 289
268 80 74 37
AflII C'TTAA_G
Cuts at: 820 1017 4181 820
Size: 197 3164 4881
Fragments arranged by size:
4881 3164 197
AflIII A'CryG_T
Cuts at: 1203 2736 3012 4335 7141 8118 1203
Size: 1533 276 1323 2806 977 1327
Fragments arranged by size:
2806 1533 1327 1323 977 276
AgeI A'CCGG_T
Cuts at: 4605 7073 4605
Size: 2468 5774
AhdI GACnn_n'nnGTC
Cuts at: 3214 4687 6971 3214
Size: 1473 2284 4485
Fragments arranged by size:
4485 2284 1473
AleI CACnn'nnGTG
Cuts at: 1166 3905 5166 5313 1166
Size: 2739 1261 147 4095
Fragments arranged by size:
4095 2739 1261 147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8242
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2345 2377 2345
Size: 32 8210
AlwNI CAG_nnn'CTG
Cuts at: 4567 5985 7557 4567
Size: 1418 1572 5252
Fragments arranged by size:
5252 1572 1418
ApaLI G'TGCA_C
Cuts at: 4601 6299 7455 4601
Size: 1698 1156 5388
Fragments arranged by size:
5388 1698 1156
ApoI r'AATT_y
Cuts at: 1493 2115 5131 5551 5777 5929 8038 1493
Size: 622 3016 420 226 152 2109 1697
Fragments arranged by size:
3016 2109 1697 622 420 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6796 160
Size: 872 5764 1606
Fragments arranged by size:
5764 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8242
AvaI C'yCGr_G
Cuts at: 1870 1943 2233 2406 3903 4489 4718 5567 1870
Size: 73 290 173 1497 586 229 849 4545
Fragments arranged by size:
4545 1497 849 586 290 229 173 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2464 2497 3793 3826 2464
Size: 33 1296 33 6880
Fragments arranged by size:
6880 1296 33 33
BamHI G'GATC_C
Cuts at: 5047 5572 5939 5047
Size: 525 367 7350
Fragments arranged by size:
7350 525 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3353 3500 3531 3809 3907
Size: 325 217 627 1566 147 31 278 98
Cuts at: 3907 5563 7018 618
Size: 1656 1455 1842
Fragments arranged by size:
1842 1656 1566 1455 627 325 278 217
147 98 31
BanII G_rGCy'C
Cuts at: 1391 2363 3129 5561 1391
Size: 972 766 2432 4072
Fragments arranged by size:
4072 2432 972 766
BbeI G_GCGC'C
Cuts at: 3535 3535
Size: 8242
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3255 3812 4472 5023 928
Size: 971 1356 557 660 551 4147
Fragments arranged by size:
4147 1356 971 660 557 551
BbvCI CC'TCA_GC
Cuts at: 2618 4662 4883 2618
Size: 2044 221 5977
Fragments arranged by size:
5977 2044 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2801 2835 6433 6467 1903
Size: 34 864 34 3598 34 3678
Fragments arranged by size:
3678 3598 864 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6134 7344 6134
Size: 1210 7032
BclI T'GATC_A
Cuts at: 1548 4785 1548
Size: 3237 5005
BfrBI ATG'CAT
Cuts at: 2430 2430
Size: 8242
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 3561 3605
Size: 107 122 71 631 605 649 1240 44
Cuts at: 3605 4294 6853 136
Size: 689 2559 1525
Fragments arranged by size:
2559 1525 1240 689 649 631 605 122
107 71 44
BglII A'GATC_T
Cuts at: 4587 8237 4587
Size: 3650 4592
BlpI GC'TnA_GC
Cuts at: 3128 4571 5659 3128
Size: 1443 1088 5711
Fragments arranged by size:
5711 1443 1088
Bme1580I G_kGCm'C
Cuts at: 1790 2993 3025 3059 3503 3920 4605 6303 7459
Size: 1203 32 34 444 417 685 1698 1156
Cuts at: 7459 1790
Size: 2573
Fragments arranged by size:
2573 1698 1203 1156 685 444 417 34
32
BmgBI CAC'GTC
Cuts at: 1318 2311 3253 4215 4336 5149 1318
Size: 993 942 962 121 813 4411
Fragments arranged by size:
4411 993 962 942 813 121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2281 3974 4279
Size: 416 53 83 399 24 864 1693 305
Cuts at: 4279 5973 6931 442
Size: 1694 958 1753
Fragments arranged by size:
1753 1694 1693 958 864 416 399 305
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8242
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2219 3148 2219
Size: 929 7313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2347 2361 3252 4016 4108 4406 5309 6902
Size: 1108 14 891 764 92 298 903 1593
Cuts at: 6902 1239
Size: 2579
Fragments arranged by size:
2579 1593 1108 903 891 764 298 92
14
Bpu10I CC'TnA_GC
Cuts at: 2618 4662 4883 2618
Size: 2044 221 5977
Fragments arranged by size:
5977 2044 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2497 3425 6367 7232 7530 2497
Size: 928 2942 865 298 3209
Fragments arranged by size:
3209 2942 928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3874 6905 882
Size: 617 2375 3031 2219
Fragments arranged by size:
3031 2375 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2503 5539 6033 493
Size: 711 1299 3036 494 2702
Fragments arranged by size:
3036 2702 1299 711 494
BsaBI GATnn'nnATC
Cuts at: 4952 8236 4952
Size: 3284 4958
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2660 3296 3532 4683
Size: 53 83 186 936 1127 636 236 1151
Cuts at: 4683 5348 6049 6431 7066 275
Size: 665 701 382 635 1451
Fragments arranged by size:
1451 1151 1127 936 701 665 636 635
382 236 186 83 53
BsaWI w'CCGG_w
Cuts at: 3148 3895 4605 6675 7073 7347 7494 7905 3148
Size: 747 710 2070 398 274 147 411 3485
Fragments arranged by size:
3485 2070 747 710 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2345 2375 2687 2717 4014
Size: 30 165 30 942 30 312 30 1297
Cuts at: 4014 4044 4379 4409 5048 5078 5198 5228 1178
Size: 30 335 30 639 30 120 30 4192
Fragments arranged by size:
4192 1297 942 639 335 312 165 120
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2708 2719 3172 3175 3880 4955 5093 5096
Size: 1522 11 453 3 705 1075 138 3
Cuts at: 5096 5219 5306 5309 1186
Size: 123 87 3 4119
Fragments arranged by size:
4119 1522 1075 705 453 138 123 87
11 3 3 3
BseYI C'CCAG_C
Cuts at: 7445 7941 7445
Size: 496 7746
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4517 4580 1163
Size: 573 2781 63 4825
Fragments arranged by size:
4825 2781 573 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2523 2683 4298 4610 5692 6453
Size: 275 186 522 160 1615 312 1082 761
Cuts at: 6453 7481 1540
Size: 1028 2301
Fragments arranged by size:
2301 1615 1082 1028 761 522 312 275
186 160
BsiHKAI G_wGCw'C
Cuts at: 2363 2529 2864 3129 4488 4605 4703 4868 6303
Size: 166 335 265 1359 117 98 165 1435
Cuts at: 6303 6388 7459 2363
Size: 85 1071 3146
Fragments arranged by size:
3146 1435 1359 1071 335 265 166 165
117 98 85
BsmI GAATG_Cn'
Cuts at: 2940 4371 5753 5846 2940
Size: 1431 1382 93 5336
Fragments arranged by size:
5336 1431 1382 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2790 3150 3869 4937 2790
Size: 360 719 1068 6095
Fragments arranged by size:
6095 1068 719 360
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2363 2529 2864 2993 3025 3059 3129
Size: 399 573 166 335 129 32 34 70
Cuts at: 3129 3503 3920 4488 4605 4703 4868 5561 6303
Size: 374 417 568 117 98 165 693 742
Cuts at: 6303 6388 7459 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 693 573 568 417 399
374 335 166 165 129 117 98 85
70 34 32
BspEI T'CCGG_A
Cuts at: 3895 7905 3895
Size: 4010 4232
BspHI T'CATG_A
Cuts at: 2493 3381 5127 5469 6131 7861 2493
Size: 888 1746 342 662 1730 2874
Fragments arranged by size:
2874 1746 1730 888 662 342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2827 4333 4575 5597 844
Size: 1983 1506 242 1022 3489
Fragments arranged by size:
3489 1983 1506 1022 242
BsrBI CCG'CTC
Cuts at: 1394 1823 2198 4597 4723 5323 6129 1394
Size: 429 375 2399 126 600 806 3507
Fragments arranged by size:
3507 2399 806 600 429 375 126
BsrDI GCAATG_nn'
Cuts at: 65 1248 6737 6911 65
Size: 1183 5489 174 1396
Fragments arranged by size:
5489 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3157 3528 4418 4605 6886 7073 1700
Size: 110 1347 371 890 187 2281 187 2869
Fragments arranged by size:
2869 2281 1347 890 371 187 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8242
BssHII G'CGCG_C
Cuts at: 1925 2274 2509 1925
Size: 349 235 7658
Fragments arranged by size:
7658 349 235
BssSI C'ACGA_G
Cuts at: 2992 6302 7314 2992
Size: 3310 1012 3920
Fragments arranged by size:
3920 3310 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2946 2997 3507 4567 794
Size: 2152 51 510 1060 4469
Fragments arranged by size:
4469 2152 1060 510 51
BstBI TT'CG_AA
Cuts at: 2007 7046 2007
Size: 5039 3203
BstEII G'GTnAC_C
Cuts at: 1184 2612 4408 1184
Size: 1428 1796 5018
Fragments arranged by size:
5018 1796 1428
BstXI CCAn_nnnn'nTGG
Cuts at: 5044 5044
Size: 8242
BstYI r'GATC_y
Cuts at: 1972 2667 4587 5047 5572 5939 6324 6341 7793
Size: 695 1920 460 525 367 385 17 1452
Cuts at: 7793 7879 7891 8237 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1920 1452 695 525 460 385 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2316 3429 3911 4899 4986 5021
Size: 552 957 294 1113 482 988 87 35
Cuts at: 5021 5271 513
Size: 250 3484
Fragments arranged by size:
3484 1113 988 957 552 482 294 250
87 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7503
Fragments arranged by size:
7503 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 4315 4505 5758 6552 6572 2944
Size: 1371 190 1253 794 20 4614
Fragments arranged by size:
4614 1371 1253 794 190 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8207
DraI TTT'AAA
Cuts at: 2089 2149 5546 2089
Size: 60 3397 4785
Fragments arranged by size:
4785 3397 60
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7249 809
Size: 507 5933 1802
Fragments arranged by size:
5933 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2313 3769 4295
Size: 54 1425 50 275 18 483 1456 526
Cuts at: 4295 4632 5226 5354 5373 5455 5689 6578 8207
Size: 337 594 128 19 82 234 889 1629
Cuts at: 8207 8
Size: 43
Fragments arranged by size:
1629 1456 1425 889 594 526 483 337
275 234 128 82 54 50 43 19
18
EagI C'GGCC_G
Cuts at: 1537 1812 4295 5689 1537
Size: 275 2483 1394 4090
Fragments arranged by size:
4090 2483 1394 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 2834 3631 4940 6172 1353
Size: 738 743 797 1309 1232 3423
Fragments arranged by size:
3423 1309 1232 797 743 738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5102 6829 7203 7349 387
Size: 1064 138 3513 1727 374 146 1280
Fragments arranged by size:
3513 1727 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2361 3127 2361
Size: 766 7476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2347 2361 2739 3252 3413 4016 4108
Size: 393 715 14 378 513 161 603 92
Cuts at: 4108 4406 4409 4489 4997 5286 5309 5360 5397
Size: 298 3 80 508 289 23 51 37
Cuts at: 5397 6305 6902 7689 7957 1239
Size: 908 597 787 268 1524
Fragments arranged by size:
1524 908 787 715 603 597 513 508
393 378 298 289 268 161 92 80
51 37 23 14 3
EcoNI CCTnn'n_nnAGG
Cuts at: 4676 4886 5161 5342 4676
Size: 210 275 181 7576
Fragments arranged by size:
7576 275 210 181
EcoO109I rG'GnC_Cy
Cuts at: 2983 2983
Size: 8242
EcoRI G'AATT_C
Cuts at: 2115 5131 5551 5929 2115
Size: 3016 420 378 4428
Fragments arranged by size:
4428 3016 420 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3642 5528 3642
Size: 1886 6356
FspI TGC'GCA
Cuts at: 6748 6748
Size: 8242
HaeII r_GCGC'y
Cuts at: 1678 3535 3936 4328 7389 1678
Size: 1857 401 392 3061 2531
Fragments arranged by size:
3061 2531 1857 401 392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2687 2719 3200 3232 3505
Size: 32 135 32 1115 32 481 32 273
Cuts at: 3505 3537 4673 4705 4770 4802 5162 5194 6883
Size: 32 1136 32 65 32 360 32 1689
Cuts at: 6883 6915 6957 6989 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1689 1136 1115 481 360 273 135
65 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5586 5832 669
Size: 988 281 3648 246 3079
Fragments arranged by size:
3648 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8242
HpaI GTT'AAC
Cuts at: 5832 5832
Size: 8242
KasI G'GCGC_C
Cuts at: 3531 3531
Size: 8242
KpnI G_GTAC'C
Cuts at: 3813 5567 3813
Size: 1754 6488
MfeI C'AATT_G
Cuts at: 5841 5841
Size: 8242
MluI A'CGCG_T
Cuts at: 8118 8118
Size: 8242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3378 4311 7356 7540 1305
Size: 2073 933 3045 184 2007
Fragments arranged by size:
3045 2073 2007 933 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2315 5228 5457 10
Size: 54 1425 343 483 2913 229 2795
Fragments arranged by size:
2913 2795 1425 483 343 229 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2811 3905 4871 4898 5166 5313 6200
Size: 648 1645 1094 966 27 268 147 887
Cuts at: 6200 6559 6718 518
Size: 359 159 2042
Fragments arranged by size:
2042 1645 1094 966 887 648 359 268
159 147 27
MspA1I CmG'CkG
Cuts at: 1606 2184 2223 2888 3507 3816 3838 3913 4384
Size: 578 39 665 619 309 22 75 471
Cuts at: 4384 4552 4791 5023 5313 5658 6335 7483 7728
Size: 168 239 232 290 345 677 1148 245
Cuts at: 7728 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 665 619 578 471 345
309 290 245 239 232 168 75 39
22
NarI GG'CG_CC
Cuts at: 3532 3532
Size: 8242
NcoI C'CATG_G
Cuts at: 513 1065 2022 2316 3429 4899 4986 5271 513
Size: 552 957 294 1113 1470 87 285 3484
Fragments arranged by size:
3484 1470 1113 957 552 294 285 87
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8242
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8242
NotI GC'GGCC_GC
Cuts at: 5689 5689
Size: 8242
NsiI A_TGCA'T
Cuts at: 2432 2432
Size: 8242
NspI r_CATG'y
Cuts at: 1132 1229 2434 2740 3016 4717 5600 7145 1132
Size: 97 1205 306 276 1701 883 1545 2229
Fragments arranged by size:
2229 1701 1545 1205 883 306 276 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8242
PciI A'CATG_T
Cuts at: 2736 3012 7141 2736
Size: 276 4129 3837
Fragments arranged by size:
4129 3837 276
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2656 4555 1108
Size: 1548 1899 4795
Fragments arranged by size:
4795 1899 1548
PfoI T'CCnGG_A
Cuts at: 2363 2873 4124 4677 2363
Size: 510 1251 553 5928
Fragments arranged by size:
5928 1251 553 510
PmeI GTTT'AAAC
Cuts at: 5546 5546
Size: 8242
PmlI CAC'GTG
Cuts at: 2503 2503
Size: 8242
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2345 2377 5109 5141 6311 6343 7824 7856 2345
Size: 32 2732 32 1170 32 1481 32 2731
Fragments arranged by size:
2732 2731 1481 1170 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2983 2983
Size: 8242
PshAI GACnn'nnGTC
Cuts at: 1613 3394 1613
Size: 1781 6461
PsiI TTA'TAA
Cuts at: 5812 5812
Size: 8242
PspXI vC'TCGA_Gb
Cuts at: 4489 4718 4489
Size: 229 8013
PstI C_TGCA'G
Cuts at: 830 2254 2824 3616 4644 5594 830
Size: 1424 570 792 1028 950 3478
Fragments arranged by size:
3478 1424 1028 950 792 570
PvuI CG_AT'CG
Cuts at: 2001 2683 2001
Size: 682 7560
PvuII CAG'CTG
Cuts at: 2223 3507 3816 4384 4791 5313 2223
Size: 1284 309 568 407 522 5152
Fragments arranged by size:
5152 1284 568 522 407 309
SacI G_AGCT'C
Cuts at: 2363 3129 2363
Size: 766 7476
SacII CC_GC'GG
Cuts at: 3914 5024 3914
Size: 1110 7132
SalI G'TCGA_C
Cuts at: 1936 5584 1936
Size: 3648 4594
SapI GCTCTTCn'nnn_
Cuts at: 2834 4940 2834
Size: 2106 6136
SbfI CC_TGCA'GG
Cuts at: 5594 5594
Size: 8242
ScaI AGT'ACT
Cuts at: 2243 4097 6490 8202 2243
Size: 1854 2393 1712 2283
Fragments arranged by size:
2393 2283 1854 1712
SfcI C'TryA_G
Cuts at: 826 1045 2250 2706 2820 3612 4357 4640 5590
Size: 219 1205 456 114 792 745 283 950
Cuts at: 5590 6725 7406 7597 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1205 1135 950 792 745 681 456
283 219 191 114
SfiI GGCCn_nnn'nGGCC
Cuts at: 2321 2321
Size: 8242
SfoI GGC'GCC
Cuts at: 3533 3533
Size: 8242
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8242
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8242
SmaI CCC'GGG
Cuts at: 1872 2235 3905 5569 1872
Size: 363 1670 1664 4545
Fragments arranged by size:
4545 1670 1664 363
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2476 3440 4181 4489 4718 6346
Size: 197 926 533 964 741 308 229 1628
Cuts at: 6346 7247 7509 820
Size: 901 262 1553
Fragments arranged by size:
1628 1553 964 926 901 741 533 308
262 229 197
SnaBI TAC'GTA
Cuts at: 493 5539 6033 493
Size: 5046 494 2702
Fragments arranged by size:
5046 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8242
SphI G_CATG'C
Cuts at: 1132 5600 1132
Size: 4468 3774
SspI AAT'ATT
Cuts at: 5 52 6166 5
Size: 47 6114 2081
Fragments arranged by size:
6114 2081 47
StuI AGG'CCT
Cuts at: 1517 3290 5063 1517
Size: 1773 1773 4696
Fragments arranged by size:
4696 1773 1773
StyI C'CwwG_G
Cuts at: 513 1065 2022 2316 3429 4614 4899 4986 5229
Size: 552 957 294 1113 1185 285 87 243
Cuts at: 5229 5271 5452 5681 513
Size: 42 181 229 3074
Fragments arranged by size:
3074 1185 1113 957 552 294 285 243
229 181 87 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2650 3560 3993 4396 5449 6269 6286
Size: 1255 1070 910 433 403 1053 820 17
Cuts at: 6286 6439 6624 7069 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1070 1053 910 820 445 433
403 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 2289 2433
Size: 274 80 33 51 1443 264 48 144
Cuts at: 2433 3075 4095 5094 6488 8200 96
Size: 642 1020 999 1394 1712 138
Fragments arranged by size:
1712 1443 1394 1020 999 642 274 264
144 138 80 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3475 3507 4014 4046 4259 4291 4379 4411 5198
Size: 32 507 32 213 32 88 32 787
Cuts at: 5198 5230 8202 8234 3475
Size: 32 2972 32 3483
Fragments arranged by size:
3483 2972 787 507 213 88 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8242
XbaI T'CTAG_A
Cuts at: 5578 5578
Size: 8242
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4214 5470 4214
Size: 1256 6986
XhoI C'TCGA_G
Cuts at: 1943 4489 4718 1943
Size: 2546 229 5467
Fragments arranged by size:
5467 2546 229
XmaI C'CCGG_G
Cuts at: 1870 2233 3903 5567 1870
Size: 363 1670 1664 4545
Fragments arranged by size:
4545 1670 1664 363
XmnI GAAnn'nnTTC
Cuts at: 2447 2900 2957 3563 6371 2447
Size: 453 57 606 2808 4318
Fragments arranged by size:
4318 2808 606 453 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4684 6050 276
Size: 53 83 186 936 3150 1366 2468
Fragments arranged by size:
3150 2468 1366 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BsiWI BstZ17I Bsu36I DraIII
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspOMI
PsrI RsrII SanDI SexAI SrfI SwaI