Restriction map for
pFN21ASDA0636
(Circular) (Six-base) MAPSORT of: pFN21ASDA0636.seq Check: 8827 from: 1 to: 6340
pFN21ASDA0636 6340 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3553 3553
Size: 6340
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4150 278
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3683 1937
Size: 1746 4594
Acc65I G'GTAC_C
Cuts at: 3661 3661
Size: 6340
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2439 3388 4403 5787 6055 1632
Size: 807 949 1015 1384 268 1917
Fragments arranged by size:
1917 1384 1015 949 807 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 5239 6216 1203
Size: 4036 977 1327
Fragments arranged by size:
4036 1327 977
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 2938 5069 2938
Size: 2131 4209
AleI CACnn'nnGTG
Cuts at: 1166 2045 1166
Size: 879 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6340
AlwNI CAG_nnn'CTG
Cuts at: 4083 5655 4083
Size: 1572 4768
ApaLI G'TGCA_C
Cuts at: 4397 5553 4397
Size: 1156 5184
ApoI r'AATT_y
Cuts at: 1493 2249 2513 2759 2809 2893 3101 3649 3875
Size: 756 264 246 50 84 208 548 226
Cuts at: 3875 4027 6136 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 756 548 264 246 226 208
152 84 50
AseI AT'TA_AT
Cuts at: 160 1032 4894 160
Size: 872 3862 1606
Fragments arranged by size:
3862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 3665 1870
Size: 73 1722 4545
Fragments arranged by size:
4545 1722 73
BamHI G'GATC_C
Cuts at: 3670 4037 3670
Size: 367 5973
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3661 5116 618
Size: 325 217 627 1874 1455 1842
Fragments arranged by size:
1874 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3659 1391
Size: 2268 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3170 928
Size: 971 1271 4098
Fragments arranged by size:
4098 1271 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4531 4565 1903
Size: 34 2594 34 3678
Fragments arranged by size:
3678 2594 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4232 5442 4232
Size: 1210 5130
BclI T'GATC_A
Cuts at: 1548 3312 1548
Size: 1764 4576
BfrBI ATG'CAT
Cuts at: 2769 2769
Size: 6340
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2973 4951 136
Size: 107 122 71 631 605 1301 1978 1525
Fragments arranged by size:
1978 1525 1301 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2607 6335 2607
Size: 3728 2612
BlpI GC'TnA_GC
Cuts at: 3757 3757
Size: 6340
Bme1580I G_kGCm'C
Cuts at: 1790 4401 5557 1790
Size: 2611 1156 2573
Fragments arranged by size:
2611 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6340
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2026 4071 5029
Size: 416 53 83 399 24 609 2045 958
Cuts at: 5029 442
Size: 1753
Fragments arranged by size:
2045 1753 958 609 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2725 2928 3340 5000 1239
Size: 1486 203 412 1660 2579
Fragments arranged by size:
2579 1660 1486 412 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4465 5330 5628 4465
Size: 865 298 5177
Fragments arranged by size:
5177 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2946 5003 882
Size: 617 1447 2057 2219
Fragments arranged by size:
2219 2057 1447 617
BsaAI yAC'GTr
Cuts at: 493 1204 3232 3637 4131 493
Size: 711 2028 405 494 2702
Fragments arranged by size:
2702 2028 711 494 405
BsaBI GATnn'nnATC
Cuts at: 6334 6334
Size: 6340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4147 4529 5164 275
Size: 53 83 186 936 2614 382 635 1451
Fragments arranged by size:
2614 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4773 5171 5445 5592 6003 4773
Size: 398 274 147 411 5110
Fragments arranged by size:
5110 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6115
Fragments arranged by size:
6115 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3069 1186
Size: 1883 4457
BseYI C'CCAG_C
Cuts at: 3558 3610 5543 6039 3558
Size: 52 1933 496 3859
Fragments arranged by size:
3859 1933 496 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5767
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3790 4551 5579 1540
Size: 275 186 1789 761 1028 2301
Fragments arranged by size:
2301 1789 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4401 4486 5557 4401
Size: 85 1071 5184
Fragments arranged by size:
5184 1071 85
BsmI GAATG_Cn'
Cuts at: 2771 2858 3517 3851 3944 2771
Size: 87 659 334 93 5167
Fragments arranged by size:
5167 659 334 93 87
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3659 4401 4486 5557 1391
Size: 399 1869 742 85 1071 2174
Fragments arranged by size:
2174 1869 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6003 6003
Size: 6340
BspHI T'CATG_A
Cuts at: 4229 5959 4229
Size: 1730 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2395 3079 3553 3695 844
Size: 1551 684 474 142 3489
Fragments arranged by size:
3489 1551 684 474 142
BsrBI CCG'CTC
Cuts at: 1394 1823 3447 4227 1394
Size: 429 1624 780 3507
Fragments arranged by size:
3507 1624 780 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3145 4835 5009 65
Size: 1183 1897 1690 174 1396
Fragments arranged by size:
1897 1690 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4984 5171 1700
Size: 110 3174 187 2869
Fragments arranged by size:
3174 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6340
BssSI C'ACGA_G
Cuts at: 4400 5412 4400
Size: 1012 5328
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6340
BstBI TT'CG_AA
Cuts at: 2007 5144 2007
Size: 3137 3203
BstEII G'GTnAC_C
Cuts at: 1184 2930 1184
Size: 1746 4594
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2470 2656 2077
Size: 393 186 5761
Fragments arranged by size:
5761 393 186
BstYI r'GATC_y
Cuts at: 1972 2482 2607 3670 4037 4422 4439 5891 5977
Size: 510 125 1063 367 385 17 1452 86
Cuts at: 5977 5989 6335 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1063 510 385 367 346 125
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3411 513
Size: 552 957 1389 3442
Fragments arranged by size:
3442 1389 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5601
Fragments arranged by size:
5601 683 56
BtsI GCAGTG_nn'
Cuts at: 3856 4650 4670 3856
Size: 794 20 5526
Fragments arranged by size:
5526 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6305
DraI TTT'AAA
Cuts at: 3644 3644
Size: 6340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5347 809
Size: 507 4031 1802
Fragments arranged by size:
4031 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3234 3465 3787
Size: 54 1425 50 275 18 1404 231 322
Cuts at: 3787 4676 6305 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1404 889 322 275 231 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3787 1537
Size: 275 1975 4090
Fragments arranged by size:
4090 1975 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4270 1353
Size: 2917 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4927 5301 5447 387
Size: 1064 138 3338 374 146 1280
Fragments arranged by size:
3338 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2439 2725 2928 3340 3388 4403 5000
Size: 393 807 286 203 412 48 1015 597
Cuts at: 5000 5787 6055 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1015 807 787 597 412 393 286
268 203 48
EcoRI G'AATT_C
Cuts at: 2513 2809 3649 4027 2513
Size: 296 840 378 4826
Fragments arranged by size:
4826 840 378 296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 3545 2200
Size: 1345 4995
FspI TGC'GCA
Cuts at: 4846 4846
Size: 6340
HaeII r_GCGC'y
Cuts at: 1678 2054 3069 3411 5487 1678
Size: 376 1015 342 2076 2531
Fragments arranged by size:
2531 2076 1015 376 342
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2924 2956 3064
Size: 32 135 32 506 32 814 32 108
Cuts at: 3064 3096 3259 3291 3465 3497 4981 5013 5055
Size: 32 163 32 174 32 1484 32 42
Cuts at: 5055 5087 1373
Size: 32 2626
Fragments arranged by size:
2626 1484 814 506 174 163 135 108
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2141 3684 3930 669
Size: 988 281 203 1543 246 3079
Fragments arranged by size:
3079 1543 988 281 246 203
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6340
HpaI GTT'AAC
Cuts at: 3930 3930
Size: 6340
KpnI G_GTAC'C
Cuts at: 3665 3665
Size: 6340
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 6216 6216
Size: 6340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3364 3370 5454 5638 1305
Size: 2059 6 2084 184 2007
Fragments arranged by size:
2084 2059 2007 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3236 3467 10
Size: 54 1425 343 1404 231 2883
Fragments arranged by size:
2883 1425 1404 343 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2654 4298 4657 4816 518
Size: 648 879 609 1644 359 159 2042
Fragments arranged by size:
2042 1644 879 648 609 359 159
MspA1I CmG'CkG
Cuts at: 1606 2417 2890 3756 4433 5581 5826 1606
Size: 811 473 866 677 1148 245 2120
Fragments arranged by size:
2120 1148 866 811 677 473 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 3411 513
Size: 552 957 1389 3442
Fragments arranged by size:
3442 1389 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6340
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6340
NotI GC'GGCC_GC
Cuts at: 3787 3787
Size: 6340
NsiI A_TGCA'T
Cuts at: 2771 2771
Size: 6340
NspI r_CATG'y
Cuts at: 1132 1229 3698 5243 1132
Size: 97 2469 1545 2229
Fragments arranged by size:
2469 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6340
PciI A'CATG_T
Cuts at: 5239 5239
Size: 6340
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3056 3565 1108
Size: 1948 509 3883
Fragments arranged by size:
3883 1948 509
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PmlI CAC'GTG
Cuts at: 3232 3232
Size: 6340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4409 4441 5922 5954 4409
Size: 32 1481 32 4795
Fragments arranged by size:
4795 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6340
PsiI TTA'TAA
Cuts at: 3910 3910
Size: 6340
PstI C_TGCA'G
Cuts at: 830 2392 3253 3692 830
Size: 1562 861 439 3478
Fragments arranged by size:
3478 1562 861 439
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
PvuII CAG'CTG
Cuts at: 2417 2890 2417
Size: 473 5867
SalI G'TCGA_C
Cuts at: 1936 3682 1936
Size: 1746 4594
SbfI CC_TGCA'GG
Cuts at: 2392 3692 2392
Size: 1300 5040
ScaI AGT'ACT
Cuts at: 4588 6300 4588
Size: 1712 4628
SfcI C'TryA_G
Cuts at: 826 1045 2388 3249 3342 3688 4823 5504 5695
Size: 219 1343 861 93 346 1135 681 191
Cuts at: 5695 826
Size: 1471
Fragments arranged by size:
1471 1343 1135 861 681 346 219 191
93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 3667 1872
Size: 1795 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4444 5345 5607 820
Size: 197 926 2501 901 262 1553
Fragments arranged by size:
2501 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3637 4131 493
Size: 3144 494 2702
Fragments arranged by size:
3144 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6340
SphI G_CATG'C
Cuts at: 1132 3698 1132
Size: 2566 3774
SspI AAT'ATT
Cuts at: 5 52 3281 4264 5
Size: 47 3229 983 2081
Fragments arranged by size:
3229 2081 983 47
StuI AGG'CCT
Cuts at: 1517 2599 1517
Size: 1082 5258
StyI C'CwwG_G
Cuts at: 513 1065 2022 2326 2936 3148 3411 3779 513
Size: 552 957 304 610 212 263 368 3074
Fragments arranged by size:
3074 957 610 552 368 304 263 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3159 4367 4384 4537 4722 5167 325
Size: 1255 1579 1208 17 153 185 445 1498
Fragments arranged by size:
1579 1498 1255 1208 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4586 6298 96
Size: 274 80 33 51 1443 2609 1712 138
Fragments arranged by size:
2609 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6300 6332 6300
Size: 32 6308
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6340
XbaI T'CTAG_A
Cuts at: 3676 3676
Size: 6340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3418 3418
Size: 6340
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6340
XmaI C'CCGG_G
Cuts at: 1870 3665 1870
Size: 1795 4545
XmnI GAAnn'nnTTC
Cuts at: 2235 4469 2235
Size: 2234 4106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4148 276
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI