Restriction map for
pFN21ASDA0634
(Circular) (Six-base) MAPSORT of: pFN21ASDA0634.seq Check: 8235 from: 1 to: 8593
pFN21ASDA0634 8593 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3877 4622 5001 3877
Size: 745 379 7469
Fragments arranged by size:
7469 745 379
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3071 6403 278
Size: 53 83 186 936 1535 3332 2468
Fragments arranged by size:
3332 2468 1535 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3623 5936 1937
Size: 1686 2313 4594
Fragments arranged by size:
4594 2313 1686
Acc65I G'GTAC_C
Cuts at: 5914 5914
Size: 8593
AclI AA'CG_TT
Cuts at: 1563 6720 7093 1563
Size: 5157 373 3063
Fragments arranged by size:
5157 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 5331 6656 8040 8308 1632
Size: 605 3094 1325 1384 268 1917
Fragments arranged by size:
3094 1917 1384 1325 605 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8396
AflIII A'CryG_T
Cuts at: 1203 5828 5862 7492 8469 1203
Size: 4625 34 1630 977 1327
Fragments arranged by size:
4625 1630 1327 977 34
AgeI A'CCGG_T
Cuts at: 7424 7424
Size: 8593
AhdI GACnn_n'nnGTC
Cuts at: 3805 5436 7322 3805
Size: 1631 1886 5076
Fragments arranged by size:
5076 1886 1631
AleI CACnn'nnGTG
Cuts at: 1166 3513 4874 1166
Size: 2347 1361 4885
Fragments arranged by size:
4885 2347 1361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2435 3164 1184
Size: 1251 729 6613
Fragments arranged by size:
6613 1251 729
AlwNI CAG_nnn'CTG
Cuts at: 2518 3528 4027 4654 4759 5210 6336 7908 2518
Size: 1010 499 627 105 451 1126 1572 3203
Fragments arranged by size:
3203 1572 1126 1010 627 499 451 105
ApaI G_GGCC'C
Cuts at: 4148 5649 4148
Size: 1501 7092
ApaLI G'TGCA_C
Cuts at: 2498 4953 6650 7806 2498
Size: 2455 1697 1156 3285
Fragments arranged by size:
3285 2455 1697 1156
ApoI r'AATT_y
Cuts at: 1493 4172 5228 5902 6128 6280 8389 1493
Size: 2679 1056 674 226 152 2109 1697
Fragments arranged by size:
2679 2109 1697 1056 674 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7147 160
Size: 872 6115 1606
Fragments arranged by size:
6115 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8593
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2098 2335 3272 3898 5918 1870
Size: 73 119 36 237 937 626 2020 4545
Fragments arranged by size:
4545 2020 937 626 237 119 73 36
AvrII C'CTAG_G
Cuts at: 2927 2927
Size: 8593
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5494 5527 5494
Size: 33 8560
BamHI G'GATC_C
Cuts at: 5923 6290 5923
Size: 367 8226
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2171 2276 2357 2528
Size: 325 217 627 249 135 105 81 171
Cuts at: 2528 4900 5914 7369 618
Size: 2372 1014 1455 1842
Fragments arranged by size:
2372 1842 1455 1014 627 325 249 217
171 135 105 81
BanII G_rGCy'C
Cuts at: 1391 2070 2199 2342 2604 3077 4148 4365 4611
Size: 679 129 143 262 473 1071 217 246
Cuts at: 4611 4707 4868 5046 5103 5352 5649 5912 1391
Size: 96 161 178 57 249 297 263 4072
Fragments arranged by size:
4072 1071 679 473 297 263 262 249
246 217 178 161 143 129 96 57
BbeI G_GCGC'C
Cuts at: 2040 2175 2280 2040
Size: 135 105 8353
Fragments arranged by size:
8353 135 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2225 3747 3801 4240 928
Size: 971 326 1522 54 439 5281
Fragments arranged by size:
5281 1522 971 439 326 54
BbvCI CC'TCA_GC
Cuts at: 5021 5021
Size: 8593
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2711 2745 6784 6818 1903
Size: 34 774 34 4039 34 3678
Fragments arranged by size:
4039 3678 774 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5380 6485 7695 5380
Size: 1105 1210 6278
Fragments arranged by size:
6278 1210 1105
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2929 4848 7204
Size: 107 122 71 631 605 1257 1919 2356
Cuts at: 7204 136
Size: 1525
Fragments arranged by size:
2356 1919 1525 1257 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5481 8588 5481
Size: 3107 5486
BlpI GC'TnA_GC
Cuts at: 2045 4472 4862 5411 6010 2045
Size: 2427 390 549 599 4628
Fragments arranged by size:
4628 2427 599 549 390
Bme1580I G_kGCm'C
Cuts at: 1790 2502 2871 3273 3914 4148 4957 5270 5649
Size: 712 369 402 641 234 809 313 379
Cuts at: 5649 5751 6654 7810 1790
Size: 102 903 1156 2573
Fragments arranged by size:
2573 1156 903 809 712 641 402 379
369 313 234 102
BmgBI CAC'GTC
Cuts at: 1318 2545 1318
Size: 1227 7366
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3155 3538 6324
Size: 416 53 83 399 24 1738 383 2786
Cuts at: 6324 7282 442
Size: 958 1753
Fragments arranged by size:
2786 1753 1738 958 416 399 383 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2290 2483 2524 2540 2579 4410 4646 5089
Size: 1051 193 41 16 39 1831 236 443
Cuts at: 5089 5366 5644 5686 5699 7253 1239
Size: 277 278 42 13 1554 2579
Fragments arranged by size:
2579 1831 1554 1051 443 278 277 236
193 42 41 39 16 13
Bpu10I CC'TnA_GC
Cuts at: 4978 5021 4978
Size: 43 8550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3587 4496 4691 5795 6718 7583 7881 3587
Size: 909 195 1104 923 865 298 4299
Fragments arranged by size:
4299 1104 923 909 865 298 195
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4670 5122 5167 5363 5557 7256 882
Size: 617 3171 452 45 196 194 1699 2219
Fragments arranged by size:
3171 2219 1699 617 452 196 194 45
BsaAI yAC'GTr
Cuts at: 493 1204 5829 5865 5890 6384 493
Size: 711 4625 36 25 494 2702
Fragments arranged by size:
4625 2702 711 494 36 25
BsaBI GATnn'nnATC
Cuts at: 2630 8587 2630
Size: 5957 2636
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 2172 2277 3068
Size: 53 83 186 936 504 135 105 791
Cuts at: 3068 6400 6782 7417 275
Size: 3332 382 635 1451
Fragments arranged by size:
3332 1451 936 791 635 504 382 186
135 105 83 53
BsaWI w'CCGG_w
Cuts at: 3458 4218 4369 4446 7026 7424 7698 7845 8256
Size: 760 151 77 2580 398 274 147 411
Cuts at: 8256 3458
Size: 3795
Fragments arranged by size:
3795 2580 760 411 398 274 151 147
77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2930 2960 3944 3974 4325
Size: 30 165 30 1527 30 984 30 351
Cuts at: 4325 4355 4535 4556 4565 4586 4694 4724 1178
Size: 30 180 21 9 21 108 30 5047
Fragments arranged by size:
5047 1527 984 351 180 165 108 30
30 30 30 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2633 2924 2933 3932 4100 4349 4556 4817
Size: 1447 291 9 999 168 249 207 261
Cuts at: 4817 5786 5849 1186
Size: 969 63 3930
Fragments arranged by size:
3930 1447 999 969 291 261 249 207
168 63 9
BseYI C'CCAG_C
Cuts at: 2297 2709 2800 4224 4293 4631 4687 5641 7796
Size: 412 91 1424 69 338 56 954 2155
Cuts at: 7796 8292 2297
Size: 496 2598
Fragments arranged by size:
2598 2155 1424 954 496 412 338 91
69 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 3487 3838 4451 5029 1163
Size: 573 705 1046 351 613 578 4727
Fragments arranged by size:
4727 1046 705 613 578 573 351
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2081 6043 6804 7832 1540
Size: 275 186 80 3962 761 1028 2301
Fragments arranged by size:
3962 2301 1028 761 275 186 80
BsiHKAI G_wGCw'C
Cuts at: 2502 3279 3732 4058 4611 4707 4957 4979 6654
Size: 777 453 326 553 96 250 22 1675
Cuts at: 6654 6739 7810 2502
Size: 85 1071 3285
Fragments arranged by size:
3285 1675 1071 777 553 453 326 250
96 85 22
BsiWI C'GTAC_G
Cuts at: 5865 5865
Size: 8593
BsmI GAATG_Cn'
Cuts at: 6104 6197 6104
Size: 93 8500
BsmBI CGTCTCn'nnnn_
Cuts at: 2551 2551
Size: 8593
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2199 2342 2502 2604 2871 3077
Size: 399 280 129 143 160 102 267 206
Cuts at: 3077 3273 3279 3732 3914 4058 4148 4365 4611
Size: 196 6 453 182 144 90 217 246
Cuts at: 4611 4707 4868 4957 4979 5046 5103 5270 5352
Size: 96 161 89 22 67 57 167 82
Cuts at: 5352 5649 5751 5912 6654 6739 7810 1391
Size: 297 102 161 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 399 297 280 267
246 217 206 196 182 167 161 161
160 144 143 129 102 102 96 90
89 85 82 67 57 22 6
BspEI T'CCGG_A
Cuts at: 4369 4446 8256 4369
Size: 77 3810 4706
Fragments arranged by size:
4706 3810 77
BspHI T'CATG_A
Cuts at: 2770 3475 6482 8212 2770
Size: 705 3007 1730 3151
Fragments arranged by size:
3151 3007 1730 705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3810 3877 4622 5001 5948 844
Size: 2966 67 745 379 947 3489
Fragments arranged by size:
3489 2966 947 745 379 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2274 6480 1394
Size: 429 451 4206 3507
Fragments arranged by size:
4206 3507 451 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7088 7262 65
Size: 1183 5840 174 1396
Fragments arranged by size:
5840 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 2168 2211 2354 3882 4327 4842
Size: 110 223 135 43 143 1528 445 515
Cuts at: 4842 5071 5883 7237 7424 1700
Size: 229 812 1354 187 2869
Fragments arranged by size:
2869 1528 1354 812 515 445 229 223
187 143 135 110 43
BsrGI T'GTAC_A
Cuts at: 96 5217 5493 96
Size: 5121 276 3196
Fragments arranged by size:
5121 3196 276
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8593
BssSI C'ACGA_G
Cuts at: 5391 6653 7665 5391
Size: 1262 1012 6319
Fragments arranged by size:
6319 1262 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3975 4027 5074 794
Size: 3181 52 1047 4313
Fragments arranged by size:
4313 3181 1047 52
BstBI TT'CG_AA
Cuts at: 2007 7397 2007
Size: 5390 3203
BstEII G'GTnAC_C
Cuts at: 1184 2974 4418 5419 1184
Size: 1790 1444 1001 4358
Fragments arranged by size:
4358 1790 1444 1001
BstXI CCAn_nnnn'nTGG
Cuts at: 4568 4687 4928 5113 4568
Size: 119 241 185 8048
Fragments arranged by size:
8048 241 185 119
BstYI r'GATC_y
Cuts at: 1972 2775 4515 5481 5923 6290 6675 6692 8144
Size: 803 1740 966 442 367 385 17 1452
Cuts at: 8144 8230 8242 8588 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1740 1452 966 803 442 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3915 5426 3915
Size: 1511 7082
BtgI C'CryG_G
Cuts at: 513 1065 2022 2694 3213 3392 4413 4425 5296
Size: 552 957 672 519 179 1021 12 871
Cuts at: 5296 513
Size: 3810
Fragments arranged by size:
3810 1021 957 871 672 552 519 179
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2398 2647 3782 4567 487
Size: 683 56 1172 249 1135 785 4513
Fragments arranged by size:
4513 1172 1135 785 683 249 56
BtsI GCAGTG_nn'
Cuts at: 2111 2237 2519 3529 4346 4442 5695 6109 6903
Size: 126 282 1010 817 96 1253 414 794
Cuts at: 6903 6923 2111
Size: 20 3781
Fragments arranged by size:
3781 1253 1010 817 794 414 282 126
96 20
ClaI AT'CG_AT
Cuts at: 1554 4998 1554
Size: 3444 5149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3766 3801 529
Size: 35 3202 35 5321
Fragments arranged by size:
5321 3202 35 35
DraI TTT'AAA
Cuts at: 5897 5897
Size: 8593
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7600 809
Size: 507 6284 1802
Fragments arranged by size:
6284 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2078 2166
Size: 54 1425 50 275 18 195 53 88
Cuts at: 2166 3210 3351 3708 4299 4844 6040 6929 8558
Size: 1044 141 357 591 545 1196 889 1629
Cuts at: 8558 8
Size: 43
Fragments arranged by size:
1629 1425 1196 1044 889 591 545 357
275 195 141 88 54 53 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2078 6040 1537
Size: 275 266 3962 4090
Fragments arranged by size:
4090 3962 275 266
EarI CTCTTCn'nnn_
Cuts at: 1353 2741 3006 6523 1353
Size: 1388 265 3517 3423
Fragments arranged by size:
3517 3423 1388 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2498 3669 7180 7554 7700 387
Size: 1064 138 909 1171 3511 374 146 1280
Fragments arranged by size:
3511 1280 1171 1064 909 374 146 138
EcoICRI GAG'CTC
Cuts at: 4609 4705 4609
Size: 96 8497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2290 2483 2524 2540 2579 4410
Size: 393 605 53 193 41 16 39 1831
Cuts at: 4410 4646 5089 5331 5366 5644 5686 5699 6656
Size: 236 443 242 35 278 42 13 957
Cuts at: 6656 7253 8040 8308 1239
Size: 597 787 268 1524
Fragments arranged by size:
1831 1524 957 787 605 597 443 393
278 268 242 236 193 53 42 41
39 35 16 13
EcoNI CCTnn'n_nnAGG
Cuts at: 4523 4523
Size: 8593
EcoO109I rG'GnC_Cy
Cuts at: 2804 3403 3545 4144 4145 4351 4591 5305 5645
Size: 599 142 599 1 206 240 714 340
Cuts at: 5645 2804
Size: 5752
Fragments arranged by size:
5752 714 599 599 340 240 206 142
1
EcoRI G'AATT_C
Cuts at: 5228 5902 6280 5228
Size: 674 378 7541
Fragments arranged by size:
7541 674 378
EcoRV GAT'ATC
Cuts at: 4396 4396
Size: 8593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3040 4960 3040
Size: 1920 6673
FspI TGC'GCA
Cuts at: 3521 7099 3521
Size: 3578 5015
HaeII r_GCGC'y
Cuts at: 1678 2040 2175 2280 2702 4816 5028 7740 1678
Size: 362 135 105 422 2114 212 2712 2531
Fragments arranged by size:
2712 2531 2114 422 362 212 135 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2968 3000 3223 3255 3791
Size: 32 135 32 1396 32 223 32 536
Cuts at: 3791 3823 4097 4129 4535 4567 4694 4726 5240
Size: 32 274 32 406 32 127 32 514
Cuts at: 5240 5272 5422 5454 5473 5505 7234 7266 7308
Size: 32 150 32 19 32 1729 32 42
Cuts at: 7308 7340 1373
Size: 32 2626
Fragments arranged by size:
2626 1729 1396 536 514 406 274 223
150 135 127 42 32 32 32 32
32 32 32 32 32 32 32 32
32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 5937 6183 669
Size: 988 281 3999 246 3079
Fragments arranged by size:
3999 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3356 3667 748
Size: 2608 311 5674
Fragments arranged by size:
5674 2608 311
HpaI GTT'AAC
Cuts at: 6183 6183
Size: 8593
KasI G'GCGC_C
Cuts at: 2036 2171 2276 2036
Size: 135 105 8353
Fragments arranged by size:
8353 135 105
KpnI G_GTAC'C
Cuts at: 5918 5918
Size: 8593
MfeI C'AATT_G
Cuts at: 6192 6192
Size: 8593
MluI A'CGCG_T
Cuts at: 8469 8469
Size: 8593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3360 4402 5190 7707 7891 1305
Size: 2055 1042 788 2517 184 2007
Fragments arranged by size:
2517 2055 2007 1042 788 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3212 3710 10
Size: 54 1425 343 1380 498 4893
Fragments arranged by size:
4893 1425 1380 498 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 2645 2864 3513 4064 4874 5543
Size: 648 1251 228 219 649 551 810 669
Cuts at: 5543 6551 6910 7069 518
Size: 1008 359 159 2042
Fragments arranged by size:
2042 1251 1008 810 669 649 648 551
359 228 219 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2161 2183 2480 2569 3100 3471 3714
Size: 516 39 22 297 89 531 371 243
Cuts at: 3714 4009 4297 4324 4654 4861 5285 6009 6686
Size: 295 288 27 330 207 424 724 677
Cuts at: 6686 7834 8079 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 724 677 531 516 424 371
330 297 295 288 245 243 207 89
39 27 22
NaeI GCC'GGC
Cuts at: 2035 2170 2213 2356 4844 2035
Size: 135 43 143 2488 5784
Fragments arranged by size:
5784 2488 143 135 43
NarI GG'CG_CC
Cuts at: 2037 2172 2277 2037
Size: 135 105 8353
Fragments arranged by size:
8353 135 105
NcoI C'CATG_G
Cuts at: 513 1065 2022 3213 3392 4413 4425 5296 513
Size: 552 957 1191 179 1021 12 871 3810
Fragments arranged by size:
3810 1191 1021 957 871 552 179 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8593
NgoMIV G'CCGG_C
Cuts at: 2033 2168 2211 2354 4842 2033
Size: 135 43 143 2488 5784
Fragments arranged by size:
5784 2488 143 135 43
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8593
NotI GC'GGCC_GC
Cuts at: 6040 6040
Size: 8593
NspI r_CATG'y
Cuts at: 1132 1229 3269 4232 5951 7496 1132
Size: 97 2040 963 1719 1545 2229
Fragments arranged by size:
2229 2040 1719 1545 963 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8593
PciI A'CATG_T
Cuts at: 7492 7492
Size: 8593
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2986 5150 1108
Size: 1878 2164 4551
Fragments arranged by size:
4551 2164 1878
PfoI T'CCnGG_A
Cuts at: 2595 2874 2889 2595
Size: 279 15 8299
Fragments arranged by size:
8299 279 15
PmeI GTTT'AAAC
Cuts at: 5897 5897
Size: 8593
PmlI CAC'GTG
Cuts at: 5829 5829
Size: 8593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2812 2844 6662 6694 8175 8207 2812
Size: 32 3818 32 1481 32 3198
Fragments arranged by size:
3818 3198 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2804 3403 3545 4351 5305 2804
Size: 599 142 806 954 6092
Fragments arranged by size:
6092 954 806 599 142
PshAI GACnn'nnGTC
Cuts at: 1613 5159 1613
Size: 3546 5047
PsiI TTA'TAA
Cuts at: 6163 6163
Size: 8593
PspOMI G'GGCC_C
Cuts at: 4144 5645 4144
Size: 1501 7092
PstI C_TGCA'G
Cuts at: 830 3751 4020 5057 5070 5181 5788 5945 830
Size: 2921 269 1037 13 111 607 157 3478
Fragments arranged by size:
3478 2921 1037 607 269 157 111 13
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8593
PvuII CAG'CTG
Cuts at: 2569 3100 3471 3714 4009 4297 4654 4861 5285
Size: 531 371 243 295 288 357 207 424
Cuts at: 5285 2569
Size: 5877
Fragments arranged by size:
5877 531 424 371 357 295 288 243
207
SacI G_AGCT'C
Cuts at: 4611 4707 4611
Size: 96 8497
SalI G'TCGA_C
Cuts at: 1936 5935 1936
Size: 3999 4594
SanDI GG'GwC_CC
Cuts at: 2804 2804
Size: 8593
SapI GCTCTTCn'nnn_
Cuts at: 2741 2741
Size: 8593
SbfI CC_TGCA'GG
Cuts at: 5788 5945 5788
Size: 157 8436
ScaI AGT'ACT
Cuts at: 5110 6841 8553 5110
Size: 1731 1712 5150
Fragments arranged by size:
5150 1731 1712
SfcI C'TryA_G
Cuts at: 826 1045 3747 4016 5053 5066 5177 5784 5941
Size: 219 2702 269 1037 13 111 607 157
Cuts at: 5941 7076 7757 7948 826
Size: 1135 681 191 1471
Fragments arranged by size:
2702 1471 1135 1037 681 607 269 219
191 157 111 13
SfoI GGC'GCC
Cuts at: 2038 2173 2278 2038
Size: 135 105 8353
Fragments arranged by size:
8353 135 105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8593
SgrAI Cr'CCGG_yG
Cuts at: 1700 2033 1700
Size: 333 8260
SmaI CCC'GGG
Cuts at: 1872 2100 2337 5920 1872
Size: 228 237 3583 4545
Fragments arranged by size:
4545 3583 237 228
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3566 4511 4706 5810 6697 7598
Size: 197 926 1623 945 195 1104 887 901
Cuts at: 7598 7860 820
Size: 262 1553
Fragments arranged by size:
1623 1553 1104 945 926 901 887 262
197 195
SnaBI TAC'GTA
Cuts at: 493 5890 6384 493
Size: 5397 494 2702
Fragments arranged by size:
5397 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8593
SphI G_CATG'C
Cuts at: 1132 5951 1132
Size: 4819 3774
SspI AAT'ATT
Cuts at: 5 52 6517 5
Size: 47 6465 2081
Fragments arranged by size:
6465 2081 47
StuI AGG'CCT
Cuts at: 1517 3645 4943 1517
Size: 2128 1298 5167
Fragments arranged by size:
5167 2128 1298
StyI C'CwwG_G
Cuts at: 513 1065 2022 2927 3060 3213 3392 3548 3640
Size: 552 957 905 133 153 179 156 92
Cuts at: 3640 4237 4413 4425 4524 5121 5157 5296 5877
Size: 597 176 12 99 597 36 139 581
Cuts at: 5877 6032 513
Size: 155 3074
Fragments arranged by size:
3074 957 905 597 597 581 552 179
176 156 155 153 139 133 99 92
36 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2642 3385 3737 5119 6620 6637 6790
Size: 1255 1062 743 352 1382 1501 17 153
Cuts at: 6790 6975 7420 325
Size: 185 445 1498
Fragments arranged by size:
1501 1498 1382 1255 1062 743 445 352
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2535 4089 5108
Size: 274 80 33 51 1443 558 1554 1019
Cuts at: 5108 5217 5493 6839 8551 96
Size: 109 276 1346 1712 138
Fragments arranged by size:
1712 1554 1443 1346 1019 558 276 274
138 109 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4325 4357 4556 4588 8553 8585 4325
Size: 32 199 32 3965 32 4333
Fragments arranged by size:
4333 3965 199 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5618 1316
Size: 4302 4291
XbaI T'CTAG_A
Cuts at: 5929 5929
Size: 8593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3703 4301 4385 3703
Size: 598 84 7911
Fragments arranged by size:
7911 598 84
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8593
XmaI C'CCGG_G
Cuts at: 1870 2098 2335 5918 1870
Size: 228 237 3583 4545
Fragments arranged by size:
4545 3583 237 228
XmnI GAAnn'nnTTC
Cuts at: 3612 4274 6722 3612
Size: 662 2448 5483
Fragments arranged by size:
5483 2448 662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3069 6401 276
Size: 53 83 186 936 1535 3332 2468
Fragments arranged by size:
3332 2468 1535 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BfrBI BplI BstZ17I DraIII FseI
FspAI NruI NsiI PacI PspXI PsrI RsrII SacII
SexAI SfiI SrfI SwaI