Restriction map for
pFN21ASDA0608
(Circular) (Six-base) MAPSORT of: pFN21ASDA0608.seq Check: 2365 from: 1 to: 7036
pFN21ASDA0608 7036 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2877 2877
Size: 7036
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4846 278
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4379 1937
Size: 2442 4594
Acc65I G'GTAC_C
Cuts at: 4357 4357
Size: 7036
AclI AA'CG_TT
Cuts at: 1563 5163 5536 1563
Size: 3600 373 3063
Fragments arranged by size:
3600 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2198 3056 5099 6483 6751 1632
Size: 566 858 2043 1384 268 1917
Fragments arranged by size:
2043 1917 1384 858 566 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6839
AflIII A'CryG_T
Cuts at: 1203 2829 5935 6912 1203
Size: 1626 3106 977 1327
Fragments arranged by size:
3106 1626 1327 977
AgeI A'CCGG_T
Cuts at: 5867 5867
Size: 7036
AhdI GACnn_n'nnGTC
Cuts at: 5765 5765
Size: 7036
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7036
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2934 3757 1184
Size: 1750 823 4463
Fragments arranged by size:
4463 1750 823
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4193 4225 4193
Size: 32 7004
AlwNI CAG_nnn'CTG
Cuts at: 2682 4779 6351 2682
Size: 2097 1572 3367
Fragments arranged by size:
3367 2097 1572
ApaLI G'TGCA_C
Cuts at: 5093 6249 5093
Size: 1156 5880
ApoI r'AATT_y
Cuts at: 1493 3160 3167 4085 4345 4571 4723 6832 1493
Size: 1667 7 918 260 226 152 2109 1697
Fragments arranged by size:
2109 1697 1667 918 260 226 152 7
AscI GG'CGCG_CC
Cuts at: 2742 2742
Size: 7036
AseI AT'TA_AT
Cuts at: 160 1032 3641 5590 160
Size: 872 2609 1949 1606
Fragments arranged by size:
2609 1949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2341 2416 2639 2659 2732 2934
Size: 73 110 288 75 223 20 73 202
Cuts at: 2934 4361 1870
Size: 1427 4545
Fragments arranged by size:
4545 1427 288 223 202 110 75 73
73 20
BamHI G'GATC_C
Cuts at: 4366 4733 4366
Size: 367 6669
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2291 2720 2890 4357
Size: 325 217 627 293 211 429 170 1467
Cuts at: 4357 5812 618
Size: 1455 1842
Fragments arranged by size:
1842 1467 1455 627 429 325 293 217
211 170
BanII G_rGCy'C
Cuts at: 1391 2259 2342 2348 2640 2941 4355 1391
Size: 868 83 6 292 301 1414 4072
Fragments arranged by size:
4072 1414 868 301 292 83 6
BbeI G_GCGC'C
Cuts at: 2295 2724 2295
Size: 429 6607
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3138 928
Size: 971 1239 4826
Fragments arranged by size:
4826 1239 971
BbvCI CC'TCA_GC
Cuts at: 3282 3282
Size: 7036
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5227 5261 1903
Size: 34 3290 34 3678
Fragments arranged by size:
3678 3290 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4928 6138 4928
Size: 1210 5826
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7036
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2203 2289 2337
Size: 107 122 71 631 605 531 86 48
Cuts at: 2337 2511 2759 5647 136
Size: 174 248 2888 1525
Fragments arranged by size:
2888 1525 631 605 531 248 174 122
107 86 71 48
BglII A'GATC_T
Cuts at: 7031 7031
Size: 7036
BlpI GC'TnA_GC
Cuts at: 4453 4453
Size: 7036
Bme1580I G_kGCm'C
Cuts at: 1790 2895 5097 6253 1790
Size: 1105 2202 1156 2573
Fragments arranged by size:
2573 2202 1156 1105
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7036
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4767 5725 442
Size: 416 53 83 399 24 3350 958 1753
Fragments arranged by size:
3350 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 2546 5696 1239
Size: 1028 279 3150 2579
Fragments arranged by size:
3150 2579 1028 279
Bpu10I CC'TnA_GC
Cuts at: 3282 3282
Size: 7036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2609 3221 3529 5161 6026 6324 2609
Size: 612 308 1632 865 298 3321
Fragments arranged by size:
3321 1632 865 612 308 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5699 882
Size: 617 4200 2219
Fragments arranged by size:
4200 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4333 4827 493
Size: 711 3129 494 2702
Fragments arranged by size:
3129 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7030 7030
Size: 7036
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 2195 2292 2325
Size: 53 83 186 936 615 47 97 33
Cuts at: 2325 2628 2721 4843 5225 5860 275
Size: 303 93 2122 382 635 1451
Fragments arranged by size:
2122 1451 936 635 615 382 303 186
97 93 83 53 47 33
BsaWI w'CCGG_w
Cuts at: 2032 5469 5867 6141 6288 6699 2032
Size: 3437 398 274 147 411 2369
Fragments arranged by size:
3437 2369 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3112 3142 1178
Size: 30 165 30 1709 30 5072
Fragments arranged by size:
5072 1709 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2198 2201 2204 2750 1186
Size: 1000 12 3 3 546 5472
Fragments arranged by size:
5472 1000 546 12 3 3
BseYI C'CCAG_C
Cuts at: 2335 6239 6735 2335
Size: 3904 496 2636
Fragments arranged by size:
3904 2636 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6463
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2235 2321 2485 2515 2763 4486
Size: 275 186 234 86 164 30 248 1723
Cuts at: 4486 5247 6275 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1723 1028 761 275 248 234 186
164 86 30
BsiHKAI G_wGCw'C
Cuts at: 3028 5097 5182 6253 3028
Size: 2069 85 1071 3811
Fragments arranged by size:
3811 2069 1071 85
BsmI GAATG_Cn'
Cuts at: 4547 4640 4547
Size: 93 6943
BsmBI CGTCTCn'nnnn_
Cuts at: 2186 2186
Size: 7036
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 2342 2348 2640 2895 2941 3028
Size: 399 469 83 6 292 255 46 87
Cuts at: 3028 4355 5097 5182 6253 1391
Size: 1327 742 85 1071 2174
Fragments arranged by size:
2174 1327 1071 742 469 399 292 255
87 85 83 46 6
BspEI T'CCGG_A
Cuts at: 2032 6699 2032
Size: 4667 2369
BspHI T'CATG_A
Cuts at: 3330 3706 4925 6655 3330
Size: 376 1219 1730 3711
Fragments arranged by size:
3711 1730 1219 376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2877 4391 844
Size: 2033 1514 3489
Fragments arranged by size:
3489 2033 1514
BsrBI CCG'CTC
Cuts at: 1394 1823 2272 4923 1394
Size: 429 449 2651 3507
Fragments arranged by size:
3507 2651 449 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3378 3783 5531 5705 65
Size: 1183 2130 405 1748 174 1396
Fragments arranged by size:
2130 1748 1396 1183 405 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2288 2477 2887 5680 5867 1700
Size: 110 478 189 410 2793 187 2869
Fragments arranged by size:
2869 2793 478 410 189 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7036
BssHII G'CGCG_C
Cuts at: 1925 2382 2471 2473 2742 1925
Size: 457 89 2 269 6219
Fragments arranged by size:
6219 457 269 89 2
BssSI C'ACGA_G
Cuts at: 5096 6108 5096
Size: 1012 6024
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3457 794
Size: 2663 4373
BstBI TT'CG_AA
Cuts at: 2007 5840 2007
Size: 3833 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7036
BstXI CCAn_nnnn'nTGG
Cuts at: 3235 3235
Size: 7036
BstYI r'GATC_y
Cuts at: 1972 4005 4366 4733 5118 5135 6587 6673 6685
Size: 2033 361 367 385 17 1452 86 12
Cuts at: 6685 7031 1972
Size: 346 1977
Fragments arranged by size:
2033 1977 1452 385 367 361 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2097 2127 2356 2387 2622 2845
Size: 552 957 75 30 229 31 235 223
Cuts at: 2845 2904 3472 513
Size: 59 568 4077
Fragments arranged by size:
4077 957 568 552 235 229 223 75
59 31 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6297
Fragments arranged by size:
6297 683 56
BtsI GCAGTG_nn'
Cuts at: 3186 4552 5346 5366 3186
Size: 1366 794 20 4856
Fragments arranged by size:
4856 1366 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7036
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7001
DraI TTT'AAA
Cuts at: 4340 4340
Size: 7036
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6043 809
Size: 507 4727 1802
Fragments arranged by size:
4727 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2281 2377
Size: 54 1425 50 275 18 402 49 96
Cuts at: 2377 2482 2512 2619 2760 4483 5372 7001 8
Size: 105 30 107 141 1723 889 1629 43
Fragments arranged by size:
1723 1629 1425 889 402 275 141 107
105 96 54 50 49 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2232 2482 2512 2760 4483 1537
Size: 275 420 250 30 248 1723 4090
Fragments arranged by size:
4090 1723 420 275 250 248 30
EarI CTCTTCn'nnn_
Cuts at: 1353 2448 3424 3807 4966 1353
Size: 1095 976 383 1159 3423
Fragments arranged by size:
3423 1159 1095 976 383
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2157 5623 5997 6143 387
Size: 1064 138 568 3466 374 146 1280
Fragments arranged by size:
3466 1280 1064 568 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2267 2546 3056 5099 5696 6483
Size: 393 566 69 279 510 2043 597 787
Cuts at: 6483 6751 1239
Size: 268 1524
Fragments arranged by size:
2043 1524 787 597 566 510 393 279
268 69
EcoO109I rG'GnC_Cy
Cuts at: 2531 2593 2729 2915 3123 2531
Size: 62 136 186 208 6444
Fragments arranged by size:
6444 208 186 136 62
EcoRI G'AATT_C
Cuts at: 4345 4723 4345
Size: 378 6658
EcoRV GAT'ATC
Cuts at: 4192 4192
Size: 7036
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3956 3956
Size: 7036
FspI TGC'GCA
Cuts at: 5542 5542
Size: 7036
HaeII r_GCGC'y
Cuts at: 1678 2157 2295 2724 2859 6183 1678
Size: 479 138 429 135 3324 2531
Fragments arranged by size:
3324 2531 479 429 138 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2185 2217 2564 2596 2833
Size: 32 135 32 613 32 347 32 237
Cuts at: 2833 2865 3197 3229 4095 4127 4181 4213 5677
Size: 32 332 32 866 32 54 32 1464
Cuts at: 5677 5709 5751 5783 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1464 866 613 347 332 237 135
54 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4380 4626 669
Size: 988 281 2442 246 3079
Fragments arranged by size:
3079 2442 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3243 748
Size: 2495 4541
HpaI GTT'AAC
Cuts at: 4626 4626
Size: 7036
KasI G'GCGC_C
Cuts at: 2291 2720 2291
Size: 429 6607
KpnI G_GTAC'C
Cuts at: 4361 4361
Size: 7036
MfeI C'AATT_G
Cuts at: 3403 4635 3403
Size: 1232 5804
MluI A'CGCG_T
Cuts at: 6912 6912
Size: 7036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3014 3630 6150 6334 1305
Size: 1709 616 2520 184 2007
Fragments arranged by size:
2520 2007 1709 616 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2283 10
Size: 54 1425 343 451 4763
Fragments arranged by size:
4763 1425 451 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3739 4232 4994 5353 5512 518
Size: 648 2573 493 762 359 159 2042
Fragments arranged by size:
2573 2042 762 648 493 359 159
MspA1I CmG'CkG
Cuts at: 1606 2245 2279 2358 2389 2599 2624 2682 2710
Size: 639 34 79 31 210 25 58 28
Cuts at: 2710 2847 2906 4452 5129 6277 6522 1606
Size: 137 59 1546 677 1148 245 2120
Fragments arranged by size:
2120 1546 1148 677 639 245 210 137
79 59 58 34 31 28 25
NaeI GCC'GGC
Cuts at: 2290 2479 2290
Size: 189 6847
NarI GG'CG_CC
Cuts at: 2292 2721 2292
Size: 429 6607
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5527
Fragments arranged by size:
5527 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7036
NgoMIV G'CCGG_C
Cuts at: 2288 2477 2288
Size: 189 6847
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7036
NotI GC'GGCC_GC
Cuts at: 4483 4483
Size: 7036
NruI TCG'CGA
Cuts at: 2445 2445
Size: 7036
NspI r_CATG'y
Cuts at: 1132 1229 3744 4394 5939 1132
Size: 97 2515 650 1545 2229
Fragments arranged by size:
2515 2229 1545 650 97
PacI TTA_AT'TAA
Cuts at: 3645 3645
Size: 7036
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7036
PciI A'CATG_T
Cuts at: 5935 5935
Size: 7036
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4254 1108
Size: 3146 3890
PmeI GTTT'AAAC
Cuts at: 4340 4340
Size: 7036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5105 5137 6618 6650 5105
Size: 32 1481 32 5491
Fragments arranged by size:
5491 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2593 2729 2915 3123 2593
Size: 136 186 208 6506
Fragments arranged by size:
6506 208 186 136
PshAI GACnn'nnGTC
Cuts at: 1613 4262 1613
Size: 2649 4387
PsiI TTA'TAA
Cuts at: 4606 4606
Size: 7036
PstI C_TGCA'G
Cuts at: 830 2687 4143 4388 830
Size: 1857 1456 245 3478
Fragments arranged by size:
3478 1857 1456 245
PvuI CG_AT'CG
Cuts at: 2001 2321 2001
Size: 320 6716
PvuII CAG'CTG
Cuts at: 2245 2682 2245
Size: 437 6599
SacII CC_GC'GG
Cuts at: 2359 2390 2625 2848 2907 2359
Size: 31 235 223 59 6488
Fragments arranged by size:
6488 235 223 59 31
SalI G'TCGA_C
Cuts at: 1936 4378 1936
Size: 2442 4594
SanDI GG'GwC_CC
Cuts at: 2729 2729
Size: 7036
SbfI CC_TGCA'GG
Cuts at: 4388 4388
Size: 7036
ScaI AGT'ACT
Cuts at: 3795 5284 6996 3795
Size: 1489 1712 3835
Fragments arranged by size:
3835 1712 1489
SfcI C'TryA_G
Cuts at: 826 1045 2683 3495 3745 4139 4384 5519 6200
Size: 219 1638 812 250 394 245 1135 681
Cuts at: 6200 6391 826
Size: 191 1471
Fragments arranged by size:
1638 1471 1135 812 681 394 250 245
219 191
SfoI GGC'GCC
Cuts at: 2293 2722 2293
Size: 429 6607
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
SgrAI Cr'CCGG_yG
Cuts at: 1700 2477 2887 1700
Size: 777 410 5849
Fragments arranged by size:
5849 777 410
SmaI CCC'GGG
Cuts at: 1872 2936 4363 1872
Size: 1064 1427 4545
Fragments arranged by size:
4545 1427 1064
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2588 3200 3544 5140 6041 6303
Size: 197 926 645 612 344 1596 901 262
Cuts at: 6303 820
Size: 1553
Fragments arranged by size:
1596 1553 926 901 645 612 344 262
197
SnaBI TAC'GTA
Cuts at: 493 4333 4827 493
Size: 3840 494 2702
Fragments arranged by size:
3840 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7036
SphI G_CATG'C
Cuts at: 1132 4394 1132
Size: 3262 3774
SrfI GCCC'GGGC
Cuts at: 2936 2936
Size: 7036
SspI AAT'ATT
Cuts at: 5 52 3361 3905 4960 5
Size: 47 3309 544 1055 2081
Fragments arranged by size:
3309 2081 1055 544 47
StuI AGG'CCT
Cuts at: 1517 3751 1517
Size: 2234 4802
StyI C'CwwG_G
Cuts at: 513 1065 2022 3769 4475 513
Size: 552 957 1747 706 3074
Fragments arranged by size:
3074 1747 957 706 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5063 5080 5233 5418 5863 325
Size: 1255 3483 17 153 185 445 1498
Fragments arranged by size:
3483 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3422 3793 5282
Size: 274 80 33 51 1443 1445 371 1489
Cuts at: 5282 6994 96
Size: 1712 138
Fragments arranged by size:
1712 1489 1445 1443 371 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6996 7028 6996
Size: 32 7004
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7036
XbaI T'CTAG_A
Cuts at: 4372 4372
Size: 7036
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3026 3026
Size: 7036
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7036
XmaI C'CCGG_G
Cuts at: 1870 2934 4361 1870
Size: 1064 1427 4545
Fragments arranged by size:
4545 1427 1064
XmnI GAAnn'nnTTC
Cuts at: 3569 4131 5165 3569
Size: 562 1034 5440
Fragments arranged by size:
5440 1034 562
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4844 276
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AvrII BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI FseI FspAI NsiI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI
SfiI SwaI