Restriction map for
pFN21ASDA0607
(Circular) (Six-base) MAPSORT of: pFN21ASDA0607.seq Check: 4223 from: 1 to: 6865
pFN21ASDA0607 6865 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2156 3103 3283 2156
Size: 947 180 5738
Fragments arranged by size:
5738 947 180
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3626 4675 278
Size: 53 83 186 936 2090 1049 2468
Fragments arranged by size:
2468 2090 1049 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4208 1937
Size: 2271 4594
Acc65I G'GTAC_C
Cuts at: 4186 4186
Size: 6865
AclI AA'CG_TT
Cuts at: 1563 4992 5365 1563
Size: 3429 373 3063
Fragments arranged by size:
3429 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2837 3713 4928 6312 6580 1632
Size: 1205 876 1215 1384 268 1917
Fragments arranged by size:
1917 1384 1215 1205 876 268
AfeI AGC'GCT
Cuts at: 2084 2084
Size: 6865
AflII C'TTAA_G
Cuts at: 820 1017 3038 820
Size: 197 2021 4647
Fragments arranged by size:
4647 2021 197
AflIII A'CryG_T
Cuts at: 1203 5764 6741 1203
Size: 4561 977 1327
Fragments arranged by size:
4561 1327 977
AgeI A'CCGG_T
Cuts at: 5696 5696
Size: 6865
AhdI GACnn_n'nnGTC
Cuts at: 5594 5594
Size: 6865
AleI CACnn'nnGTG
Cuts at: 1166 2954 3494 1166
Size: 1788 540 4537
Fragments arranged by size:
4537 1788 540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2505 2860 1184
Size: 1321 355 5189
Fragments arranged by size:
5189 1321 355
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 6833
AlwNI CAG_nnn'CTG
Cuts at: 2446 2557 2701 3034 3223 3970 4147 4608 6180
Size: 111 144 333 189 747 177 461 1572
Cuts at: 6180 2446
Size: 3131
Fragments arranged by size:
3131 1572 747 461 333 189 177 144
111
ApaI G_GGCC'C
Cuts at: 3333 3402 3430 3333
Size: 69 28 6768
Fragments arranged by size:
6768 69 28
ApaLI G'TGCA_C
Cuts at: 4922 6078 4922
Size: 1156 5709
ApoI r'AATT_y
Cuts at: 1493 4174 4400 4552 6661 1493
Size: 2681 226 152 2109 1697
Fragments arranged by size:
2681 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5419 160
Size: 872 4387 1606
Fragments arranged by size:
4387 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
AvaI C'yCGr_G
Cuts at: 1870 1943 2388 3402 3747 3808 4013 4190 1870
Size: 73 445 1014 345 61 205 177 4545
Fragments arranged by size:
4545 1014 445 345 205 177 73 61
BamHI G'GATC_C
Cuts at: 2859 3003 3162 4195 4562 2859
Size: 144 159 1033 367 5162
Fragments arranged by size:
5162 1033 367 159 144
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2251 2481 2498 3170 3375
Size: 325 217 627 464 230 17 672 205
Cuts at: 3375 4186 5641 618
Size: 811 1455 1842
Fragments arranged by size:
1842 1455 811 672 627 464 325 230
217 205 17
BanII G_rGCy'C
Cuts at: 1391 2098 2619 2691 2795 3141 3333 3402 3430
Size: 707 521 72 104 346 192 69 28
Cuts at: 3430 3693 3815 3879 4158 4184 1391
Size: 263 122 64 279 26 4072
Fragments arranged by size:
4072 707 521 346 279 263 192 122
104 72 69 64 28 26
BbeI G_GCGC'C
Cuts at: 2255 2255
Size: 6865
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5894
BbvCI CC'TCA_GC
Cuts at: 2667 2667
Size: 6865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2111 2145 2710 2744 5056 5090 1903
Size: 34 174 34 565 34 2312 34 3678
Fragments arranged by size:
3678 2312 565 174 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4757 5967 4757
Size: 1210 5655
BclI T'GATC_A
Cuts at: 1548 3414 3576 1548
Size: 1866 162 4837
Fragments arranged by size:
4837 1866 162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2303 2765 2834
Size: 107 122 71 631 605 631 462 69
Cuts at: 2834 3089 3179 3272 3305 3337 5476 136
Size: 255 90 93 33 32 2139 1525
Fragments arranged by size:
2139 1525 631 631 605 462 255 122
107 93 90 71 69 33 32
BglII A'GATC_T
Cuts at: 6860 6860
Size: 6865
BlpI GC'TnA_GC
Cuts at: 2789 4282 2789
Size: 1493 5372
Bme1580I G_kGCm'C
Cuts at: 1790 2535 2733 2951 3333 3402 3430 4926 6082
Size: 745 198 218 382 69 28 1496 1156
Cuts at: 6082 1790
Size: 2573
Fragments arranged by size:
2573 1496 1156 745 382 218 198 69
28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6865
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4596 5554 442
Size: 416 53 83 399 24 3179 958 1753
Fragments arranged by size:
3179 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3833 1056
Size: 2777 4088
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3208 3368 3208
Size: 160 6705
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2957 3705 3959 5525 1239
Size: 860 858 748 254 1566 2579
Fragments arranged by size:
2579 1566 860 858 748 254
Bpu10I CC'TnA_GC
Cuts at: 2667 3710 2667
Size: 1043 5822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4990 5855 6153 4990
Size: 865 298 5702
Fragments arranged by size:
5702 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3182 5528 882
Size: 617 1683 2346 2219
Fragments arranged by size:
2346 2219 1683 617
BsaAI yAC'GTr
Cuts at: 493 1204 3793 4162 4656 493
Size: 711 2589 369 494 2702
Fragments arranged by size:
2702 2589 711 494 369
BsaBI GATnn'nnATC
Cuts at: 3413 6859 3413
Size: 3446 3419
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2252 3588 3623 4672
Size: 53 83 186 936 719 1336 35 1049
Cuts at: 4672 5054 5689 275
Size: 382 635 1451
Fragments arranged by size:
1451 1336 1049 936 719 635 382 186
83 53 35
BsaWI w'CCGG_w
Cuts at: 5298 5696 5970 6117 6528 5298
Size: 398 274 147 411 5635
Fragments arranged by size:
5635 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3866 3896 1178
Size: 30 165 30 2463 30 4147
Fragments arranged by size:
4147 2463 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2954 3200 3275 3284 3350 3683 4118 1186
Size: 1768 246 75 9 66 333 435 3933
Fragments arranged by size:
3933 1768 435 333 246 75 66 9
BseYI C'CCAG_C
Cuts at: 3288 6068 6564 3288
Size: 2780 496 3589
Fragments arranged by size:
3589 2780 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2796 3077 3662 1163
Size: 573 1060 281 585 4366
Fragments arranged by size:
4366 1060 585 573 281
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4315 5076 6104 1540
Size: 275 186 2314 761 1028 2301
Fragments arranged by size:
2314 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 2795 3879 4926 5011 6082 2691
Size: 104 1084 1047 85 1071 3474
Fragments arranged by size:
3474 1084 1071 1047 104 85
BsmI GAATG_Cn'
Cuts at: 2744 3017 4376 4469 2744
Size: 273 1359 93 5140
Fragments arranged by size:
5140 1359 273 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2237 2936 3339 4100 2237
Size: 699 403 761 5002
Fragments arranged by size:
5002 761 699 403
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2535 2619 2691 2733 2795 2951
Size: 399 308 437 84 72 42 62 156
Cuts at: 2951 3141 3333 3402 3430 3693 3815 3879 4158
Size: 190 192 69 28 263 122 64 279
Cuts at: 4158 4184 4926 5011 6082 1391
Size: 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 437 399 308 279 263
192 190 156 122 85 84 72 69
64 62 42 28 26
BspEI T'CCGG_A
Cuts at: 6528 6528
Size: 6865
BspHI T'CATG_A
Cuts at: 3072 3618 4754 6484 3072
Size: 546 1136 1730 3453
Fragments arranged by size:
3453 1730 1136 546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2156 2631 3103 3114 3283 3539 3876 4220
Size: 1312 475 472 11 169 256 337 344
Cuts at: 4220 844
Size: 3489
Fragments arranged by size:
3489 1312 475 472 344 337 256 169
11
BsrBI CCG'CTC
Cuts at: 1394 1823 2418 4752 1394
Size: 429 595 2334 3507
Fragments arranged by size:
3507 2334 595 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2487 5360 5534 65
Size: 1183 1239 2873 174 1396
Fragments arranged by size:
2873 1396 1239 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3905 4065 5509 5696 1700
Size: 110 2095 160 1444 187 2869
Fragments arranged by size:
2869 2095 1444 187 160 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6865
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6865
BssSI C'ACGA_G
Cuts at: 3062 3554 4925 5937 3062
Size: 492 1371 1012 3990
Fragments arranged by size:
3990 1371 1012 492
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3034 3970 794
Size: 2240 936 3689
Fragments arranged by size:
3689 2240 936
BstBI TT'CG_AA
Cuts at: 2007 5669 2007
Size: 3662 3203
BstEII G'GTnAC_C
Cuts at: 1184 2971 3559 1184
Size: 1787 588 4490
Fragments arranged by size:
4490 1787 588
BstYI r'GATC_y
Cuts at: 1972 2196 2749 2859 3003 3162 3408 4195 4562
Size: 224 553 110 144 159 246 787 367
Cuts at: 4562 4947 4964 6416 6502 6514 6860 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 787 553 385 367 346 246
224 159 144 110 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5356
Fragments arranged by size:
5356 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6126
Fragments arranged by size:
6126 683 56
BtsI GCAGTG_nn'
Cuts at: 2323 3032 3467 3831 3994 4148 4381 5175 5195
Size: 709 435 364 163 154 233 794 20
Cuts at: 5195 2323
Size: 3993
Fragments arranged by size:
3993 794 709 435 364 233 163 154
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6865
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6830
DraI TTT'AAA
Cuts at: 4169 4169
Size: 6865
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5872 809
Size: 507 4556 1802
Fragments arranged by size:
4556 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2331 2628 3180
Size: 54 1425 50 275 18 501 297 552
Cuts at: 3180 3297 3306 3338 3481 3678 3959 4023 4110
Size: 117 9 32 143 197 281 64 87
Cuts at: 4110 4312 5201 6830 8
Size: 202 889 1629 43
Fragments arranged by size:
1629 1425 889 552 501 297 281 275
202 197 143 117 87 64 54 50
43 32 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 4312 1537
Size: 275 2500 4090
Fragments arranged by size:
4090 2500 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3732 3779 4795 1353
Size: 2379 47 1016 3423
Fragments arranged by size:
3423 2379 1016 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2209 2627 5452 5826 5972 387
Size: 1064 138 620 418 2825 374 146 1280
Fragments arranged by size:
2825 1280 1064 620 418 374 146 138
EcoICRI GAG'CTC
Cuts at: 2689 2793 3877 2689
Size: 104 1084 5677
Fragments arranged by size:
5677 1084 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2837 2957 3705 3713 3959 4928
Size: 393 467 738 120 748 8 246 969
Cuts at: 4928 5525 6312 6580 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 969 787 748 738 597 467 393
268 246 120 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2516 2648 3110 3257 3416 2102
Size: 414 132 462 147 159 5551
Fragments arranged by size:
5551 462 414 159 147 132
EcoO109I rG'GnC_Cy
Cuts at: 2395 2474 2554 2641 3329 3399 3426 3427 2395
Size: 79 80 87 688 70 27 1 5833
Fragments arranged by size:
5833 688 87 80 79 70 27 1
EcoRI G'AATT_C
Cuts at: 4174 4552 4174
Size: 378 6487
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3157 3157
Size: 6865
FspI TGC'GCA
Cuts at: 5371 5371
Size: 6865
HaeII r_GCGC'y
Cuts at: 1678 2086 2255 6012 1678
Size: 408 169 3757 2531
Fragments arranged by size:
3757 2531 408 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3179 3211 5506 5538 5580
Size: 32 135 32 1607 32 2295 32 42
Cuts at: 5580 5612 1373
Size: 32 2626
Fragments arranged by size:
2626 2295 1607 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4209 4455 669
Size: 988 281 2271 246 3079
Fragments arranged by size:
3079 2271 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6865
HpaI GTT'AAC
Cuts at: 4455 4455
Size: 6865
KasI G'GCGC_C
Cuts at: 2251 2251
Size: 6865
KpnI G_GTAC'C
Cuts at: 4190 4190
Size: 6865
MfeI C'AATT_G
Cuts at: 4464 4464
Size: 6865
MluI A'CGCG_T
Cuts at: 6741 6741
Size: 6865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2877 5979 6163 1305
Size: 1572 3102 184 2007
Fragments arranged by size:
3102 2007 1572 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3299 3308 3340 3483 3680
Size: 54 1425 343 1467 9 32 143 197
Cuts at: 3680 10
Size: 3195
Fragments arranged by size:
3195 1467 1425 343 197 143 54 32
9
MslI CAynn'nnrTG
Cuts at: 518 1166 2538 2954 3494 3617 4823 5182 5341
Size: 648 1372 416 540 123 1206 359 159
Cuts at: 5341 518
Size: 2042
Fragments arranged by size:
2042 1372 1206 648 540 416 359 159
123
MspA1I CmG'CkG
Cuts at: 1606 2698 3220 3764 3941 3957 3979 4281 4958
Size: 1092 522 544 177 16 22 302 677
Cuts at: 4958 6106 6351 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1092 677 544 522 302 245
177 22 16
NarI GG'CG_CC
Cuts at: 2252 2252
Size: 6865
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5356
Fragments arranged by size:
5356 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6865
NheI G'CTAG_C
Cuts at: 1052 3829 1052
Size: 2777 4088
NotI GC'GGCC_GC
Cuts at: 4312 4312
Size: 6865
NspI r_CATG'y
Cuts at: 1132 1229 3895 4223 5768 1132
Size: 97 2666 328 1545 2229
Fragments arranged by size:
2666 2229 1545 328 97
PasI CC'CwG_GG
Cuts at: 1348 3326 3685 1348
Size: 1978 359 4528
Fragments arranged by size:
4528 1978 359
PciI A'CATG_T
Cuts at: 5764 5764
Size: 6865
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2557 3295 1108
Size: 1449 738 4678
Fragments arranged by size:
4678 1449 738
PfoI T'CCnGG_A
Cuts at: 2876 3006 2876
Size: 130 6735
PmeI GTTT'AAAC
Cuts at: 4169 4169
Size: 6865
PmlI CAC'GTG
Cuts at: 3793 3793
Size: 6865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 4934 4966 6447 6479 3866
Size: 32 1036 32 1481 32 4252
Fragments arranged by size:
4252 1481 1036 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 2395
Size: 6865
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6865
PsiI TTA'TAA
Cuts at: 4435 4435
Size: 6865
PspOMI G'GGCC_C
Cuts at: 3329 3398 3426 3329
Size: 69 28 6768
Fragments arranged by size:
6768 69 28
PstI C_TGCA'G
Cuts at: 830 2318 2757 3114 3459 3528 3873 4095 4217
Size: 1488 439 357 345 69 345 222 122
Cuts at: 4217 830
Size: 3478
Fragments arranged by size:
3478 1488 439 357 345 345 222 122
69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6865
PvuII CAG'CTG
Cuts at: 2698 3220 3764 3979 2698
Size: 522 544 215 5584
Fragments arranged by size:
5584 544 522 215
SacI G_AGCT'C
Cuts at: 2691 2795 3879 2691
Size: 104 1084 5677
Fragments arranged by size:
5677 1084 104
SalI G'TCGA_C
Cuts at: 1936 4207 1936
Size: 2271 4594
SanDI GG'GwC_CC
Cuts at: 2395 2395
Size: 6865
SapI GCTCTTCn'nnn_
Cuts at: 3732 3732
Size: 6865
SbfI CC_TGCA'GG
Cuts at: 3873 4217 3873
Size: 344 6521
ScaI AGT'ACT
Cuts at: 5113 6825 5113
Size: 1712 5153
SfcI C'TryA_G
Cuts at: 826 1045 2314 2454 2753 3110 3455 3524 3869
Size: 219 1269 140 299 357 345 69 345
Cuts at: 3869 4091 4213 5348 6029 6220 826
Size: 222 122 1135 681 191 1471
Fragments arranged by size:
1471 1269 1135 681 357 345 345 299
222 219 191 140 122 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 2303 3272 3305 3337 2303
Size: 969 33 32 5831
Fragments arranged by size:
5831 969 33 32
SfoI GGC'GCC
Cuts at: 2253 2253
Size: 6865
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6865
SmaI CCC'GGG
Cuts at: 1872 2390 3404 3810 4192 1872
Size: 518 1014 406 382 4545
Fragments arranged by size:
4545 1014 518 406 382
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3038 4969 5870 6132 820
Size: 197 926 1095 1931 901 262 1553
Fragments arranged by size:
1931 1553 1095 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4162 4656 493
Size: 3669 494 2702
Fragments arranged by size:
3669 2702 494
SpeI A'CTAG_T
Cuts at: 152 3637 152
Size: 3485 3380
SphI G_CATG'C
Cuts at: 1132 3895 4223 1132
Size: 2763 328 3774
Fragments arranged by size:
3774 2763 328
SspI AAT'ATT
Cuts at: 5 52 4789 5
Size: 47 4737 2081
Fragments arranged by size:
4737 2081 47
StuI AGG'CCT
Cuts at: 1517 3899 1517
Size: 2382 4483
StyI C'CwwG_G
Cuts at: 513 1065 2022 2151 2244 4134 4304 513
Size: 552 957 129 93 1890 170 3074
Fragments arranged by size:
3074 1890 957 552 170 129 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2279 2482 3158 4892 4909 5062 5247
Size: 1255 699 203 676 1734 17 153 185
Cuts at: 5247 5692 325
Size: 445 1498
Fragments arranged by size:
1734 1498 1255 699 676 445 203 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5111 6823 96
Size: 274 80 33 51 1443 3134 1712 138
Fragments arranged by size:
3134 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3154 3186 6825 6857 3154
Size: 32 3639 32 3162
Fragments arranged by size:
3639 3162 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6865
XbaI T'CTAG_A
Cuts at: 4201 4201
Size: 6865
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3333 3333
Size: 6865
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6865
XmaI C'CCGG_G
Cuts at: 1870 2388 3402 3808 4190 1870
Size: 518 1014 406 382 4545
Fragments arranged by size:
4545 1014 518 406 382
XmnI GAAnn'nnTTC
Cuts at: 4994 4994
Size: 6865
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3624 4673 276
Size: 53 83 186 936 2090 1049 2468
Fragments arranged by size:
2468 2090 1049 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I
DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacII SexAI SrfI SwaI