Restriction map for
pFN21ASDA0601
(Circular) (Six-base) MAPSORT of: pFN21ASDA0601.seq Check: 2313 from: 1 to: 7285
pFN21ASDA0601 7285 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5095 278
Size: 53 83 186 936 3559 2468
Fragments arranged by size:
3559 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4523 4628 1937
Size: 2586 105 4594
Fragments arranged by size:
4594 2586 105
Acc65I G'GTAC_C
Cuts at: 4606 4606
Size: 7285
AclI AA'CG_TT
Cuts at: 1563 5412 5785 1563
Size: 3849 373 3063
Fragments arranged by size:
3849 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2547 3441 3530 3798 3932 5348 6732 7000
Size: 915 894 89 268 134 1416 1384 268
Cuts at: 7000 1632
Size: 1917
Fragments arranged by size:
1917 1416 1384 915 894 268 268 134
89
AflII C'TTAA_G
Cuts at: 820 1017 3668 820
Size: 197 2651 4437
Fragments arranged by size:
4437 2651 197
AflIII A'CryG_T
Cuts at: 1203 6184 7161 1203
Size: 4981 977 1327
Fragments arranged by size:
4981 1327 977
AgeI A'CCGG_T
Cuts at: 3171 6116 3171
Size: 2945 4340
AhdI GACnn_n'nnGTC
Cuts at: 6014 6014
Size: 7285
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7285
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7285
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3114 3146 4416 4448 3114
Size: 32 1270 32 5951
Fragments arranged by size:
5951 1270 32 32
AlwNI CAG_nnn'CTG
Cuts at: 5028 6600 5028
Size: 1572 5713
ApaI G_GGCC'C
Cuts at: 2162 2162
Size: 7285
ApaLI G'TGCA_C
Cuts at: 5342 6498 5342
Size: 1156 6129
ApoI r'AATT_y
Cuts at: 1493 3370 3632 4594 4820 4972 7081 1493
Size: 1877 262 962 226 152 2109 1697
Fragments arranged by size:
2109 1877 1697 962 262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5839 160
Size: 872 4807 1606
Fragments arranged by size:
4807 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7285
AvaI C'yCGr_G
Cuts at: 1870 1943 2234 4293 4610 1870
Size: 73 291 2059 317 4545
Fragments arranged by size:
4545 2059 317 291 73
BamHI G'GATC_C
Cuts at: 4036 4615 4982 4036
Size: 579 367 6339
Fragments arranged by size:
6339 579 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4385 4606 6061 618
Size: 325 217 627 2598 221 1455 1842
Fragments arranged by size:
2598 1842 1455 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2162 4095 4294 4301 4604 1391
Size: 771 1933 199 7 303 4072
Fragments arranged by size:
4072 1933 771 303 199 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6314
BbvCI CC'TCA_GC
Cuts at: 4245 4245
Size: 7285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5476 5510 1903
Size: 34 3539 34 3678
Fragments arranged by size:
3678 3539 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5177 6387 5177
Size: 1210 6075
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7285
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 2157 5896
Size: 107 122 71 631 605 382 103 3739
Cuts at: 5896 136
Size: 1525
Fragments arranged by size:
3739 1525 631 605 382 122 107 103
71
BglII A'GATC_T
Cuts at: 7280 7280
Size: 7285
BlpI GC'TnA_GC
Cuts at: 4353 4702 4353
Size: 349 6936
Bme1580I G_kGCm'C
Cuts at: 1790 2162 4390 5346 6502 1790
Size: 372 2228 956 1156 2573
Fragments arranged by size:
2573 2228 1156 956 372
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3685 5016 5974
Size: 416 53 83 399 24 2268 1331 958
Cuts at: 5974 442
Size: 1753
Fragments arranged by size:
2268 1753 1331 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5945 1239
Size: 4706 2579
Bpu10I CC'TnA_GC
Cuts at: 4245 4245
Size: 7285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2475 2577 5410 6275 6573 2475
Size: 102 2833 865 298 3187
Fragments arranged by size:
3187 2833 865 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2890 5948 882
Size: 617 1391 3058 2219
Fragments arranged by size:
3058 2219 1391 617
BsaAI yAC'GTr
Cuts at: 493 1204 4582 5076 493
Size: 711 3378 494 2702
Fragments arranged by size:
3378 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3579 7279 3579
Size: 3700 3585
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2344 5092 5474 6109
Size: 53 83 186 936 811 2748 382 635
Cuts at: 6109 275
Size: 1451
Fragments arranged by size:
2748 1451 936 811 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2335 2580 3171 5718 6116 6390 6537 6948 2335
Size: 245 591 2547 398 274 147 411 2672
Fragments arranged by size:
2672 2547 591 411 398 274 245 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3003 3033 1178
Size: 30 165 30 1600 30 5430
Fragments arranged by size:
5430 1600 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7285
BseYI C'CCAG_C
Cuts at: 2161 4444 6488 6984 2161
Size: 2283 2044 496 2462
Fragments arranged by size:
2462 2283 2044 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4257 4542 1163
Size: 573 2521 285 3906
Fragments arranged by size:
3906 2521 573 285
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4735 5496 6524 1540
Size: 275 186 2734 761 1028 2301
Fragments arranged by size:
2734 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4095 4465 5346 5431 6502 4095
Size: 370 881 85 1071 4878
Fragments arranged by size:
4878 1071 881 370 85
BsmI GAATG_Cn'
Cuts at: 4796 4889 4796
Size: 93 7192
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 4095 4294 4301 4390 4465 4604
Size: 399 372 1933 199 7 89 75 139
Cuts at: 4604 5346 5431 6502 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1933 1071 742 399 372 199 139
89 85 75 7
BspEI T'CCGG_A
Cuts at: 2335 6948 2335
Size: 4613 2672
BspHI T'CATG_A
Cuts at: 2575 5174 6904 2575
Size: 2599 1730 2956
Fragments arranged by size:
2956 2599 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4640 844
Size: 3796 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 5172 1394
Size: 429 2023 1326 3507
Fragments arranged by size:
3507 2023 1326 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5780 5954 65
Size: 1183 4532 174 1396
Fragments arranged by size:
4532 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2276 2355 3171 5929 6116 1700
Size: 110 466 79 816 2758 187 2869
Fragments arranged by size:
2869 2758 816 466 187 110 79
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7285
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7285
BssSI C'ACGA_G
Cuts at: 3736 3850 5345 6357 3736
Size: 114 1495 1012 4664
Fragments arranged by size:
4664 1495 1012 114
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7285
BstBI TT'CG_AA
Cuts at: 2007 6089 2007
Size: 4082 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7285
BstYI r'GATC_y
Cuts at: 1972 3478 4036 4615 4982 5367 5384 6836 6922
Size: 1506 558 579 367 385 17 1452 86
Cuts at: 6922 6934 7280 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1506 1452 579 558 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 4524 4524
Size: 7285
Bsu36I CC'TnA_GG
Cuts at: 2271 2502 2271
Size: 231 7054
BtgI C'CryG_G
Cuts at: 513 1065 2049 2145 2219 2310 3015 513
Size: 552 984 96 74 91 705 4783
Fragments arranged by size:
4783 984 705 552 96 91 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6546
Fragments arranged by size:
6546 683 56
BtsI GCAGTG_nn'
Cuts at: 2672 3795 4240 4801 5595 5615 2672
Size: 1123 445 561 794 20 4342
Fragments arranged by size:
4342 1123 794 561 445 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7285
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7250
DraI TTT'AAA
Cuts at: 4589 4589
Size: 7285
DraIII CAC_nnn'GTG
Cuts at: 4550 4550
Size: 7285
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3218 6292 809
Size: 507 1902 3074 1802
Fragments arranged by size:
3074 1902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2403 3081
Size: 54 1425 50 275 18 303 270 678
Cuts at: 3081 3247 4195 4206 4732 5621 7250 8
Size: 166 948 11 526 889 1629 43
Fragments arranged by size:
1629 1425 948 889 678 526 303 275
270 166 54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 4732 1537
Size: 275 2920 4090
Fragments arranged by size:
4090 2920 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2433 4100 4106 4415 5215 1353
Size: 1080 1667 6 309 800 3423
Fragments arranged by size:
3423 1667 1080 800 309 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2261 5872 6246 6392 387
Size: 1064 138 672 3611 374 146 1280
Fragments arranged by size:
3611 1280 1064 672 374 146 138
EcoICRI GAG'CTC
Cuts at: 4093 4093
Size: 7285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2547 3441 3530 3798 3932 5348 5945
Size: 393 915 894 89 268 134 1416 597
Cuts at: 5945 6732 7000 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1416 915 894 787 597 393 268
268 134 89
EcoNI CCTnn'n_nnAGG
Cuts at: 3248 3494 4001 3248
Size: 246 507 6532
Fragments arranged by size:
6532 507 246
EcoO109I rG'GnC_Cy
Cuts at: 2239 2267 2239
Size: 28 7257
EcoRI G'AATT_C
Cuts at: 4594 4972 4594
Size: 378 6907
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3371 3560 3371
Size: 189 7096
FseI GG_CCGG'CC
Cuts at: 2280 2280
Size: 7285
FspI TGC'GCA
Cuts at: 5791 5791
Size: 7285
HaeII r_GCGC'y
Cuts at: 1678 6432 1678
Size: 4754 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 2575 2607 5926
Size: 32 135 32 536 32 435 32 3319
Cuts at: 5926 5958 6000 6032 1373
Size: 32 42 32 2626
Fragments arranged by size:
3319 2626 536 435 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4629 4875 669
Size: 988 281 2691 246 3079
Fragments arranged by size:
3079 2691 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2398 2471 748
Size: 1650 73 5562
Fragments arranged by size:
5562 1650 73
HpaI GTT'AAC
Cuts at: 4875 4875
Size: 7285
KpnI G_GTAC'C
Cuts at: 4610 4610
Size: 7285
MfeI C'AATT_G
Cuts at: 4884 4884
Size: 7285
MluI A'CGCG_T
Cuts at: 7161 7161
Size: 7285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2327 2667 2752 3534 6399 6583 1305
Size: 1022 340 85 782 2865 184 2007
Fragments arranged by size:
2865 2007 1022 782 340 184 85
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2405 3083 3249 4208 10
Size: 54 1425 343 573 678 166 959 3087
Fragments arranged by size:
3087 1425 959 678 573 343 166 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5243 5602 5761 518
Size: 648 4077 359 159 2042
Fragments arranged by size:
4077 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2069 2147 2221 2707 4701 5378 6526
Size: 445 18 78 74 486 1994 677 1148
Cuts at: 6526 6771 1606
Size: 245 2120
Fragments arranged by size:
2120 1994 1148 677 486 445 245 78
74 18
NaeI GCC'GGC
Cuts at: 2278 2357 2278
Size: 79 7206
NcoI C'CATG_G
Cuts at: 513 1065 2310 3015 513
Size: 552 1245 705 4783
Fragments arranged by size:
4783 1245 705 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7285
NgoMIV G'CCGG_C
Cuts at: 2276 2355 2276
Size: 79 7206
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7285
NotI GC'GGCC_GC
Cuts at: 4732 4732
Size: 7285
NspI r_CATG'y
Cuts at: 1132 1229 3290 4064 4579 4643 6188 1132
Size: 97 2061 774 515 64 1545 2229
Fragments arranged by size:
2229 2061 1545 774 515 97 64
PasI CC'CwG_GG
Cuts at: 1348 2096 3901 4287 1348
Size: 748 1805 386 4346
Fragments arranged by size:
4346 1805 748 386
PciI A'CATG_T
Cuts at: 6184 6184
Size: 7285
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3256 3982 4510 1108
Size: 2148 726 528 3883
Fragments arranged by size:
3883 2148 726 528
PmeI GTTT'AAAC
Cuts at: 4589 4589
Size: 7285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5354 5386 6867 6899 5354
Size: 32 1481 32 5740
Fragments arranged by size:
5740 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7285
PsiI TTA'TAA
Cuts at: 2748 3870 4855 2748
Size: 1122 985 5178
Fragments arranged by size:
5178 1122 985
PspOMI G'GGCC_C
Cuts at: 2158 2158
Size: 7285
PstI C_TGCA'G
Cuts at: 830 2068 2679 3973 4321 4637 830
Size: 1238 611 1294 348 316 3478
Fragments arranged by size:
3478 1294 1238 611 348 316
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7285
PvuII CAG'CTG
Cuts at: 2707 2707
Size: 7285
SacI G_AGCT'C
Cuts at: 4095 4095
Size: 7285
SacII CC_GC'GG
Cuts at: 2052 2148 2222 2052
Size: 96 74 7115
Fragments arranged by size:
7115 96 74
SalI G'TCGA_C
Cuts at: 1936 4627 1936
Size: 2691 4594
SapI GCTCTTCn'nnn_
Cuts at: 4100 4100
Size: 7285
SbfI CC_TGCA'GG
Cuts at: 4637 4637
Size: 7285
ScaI AGT'ACT
Cuts at: 5533 7245 5533
Size: 1712 5573
SexAI A'CCwGG_T
Cuts at: 4546 4546
Size: 7285
SfcI C'TryA_G
Cuts at: 826 1045 2064 2675 3969 4317 4633 5768 6449
Size: 219 1019 611 1294 348 316 1135 681
Cuts at: 6449 6640 826
Size: 191 1471
Fragments arranged by size:
1471 1294 1135 1019 681 611 348 316
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2157 2157
Size: 7285
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7285
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7285
SmaI CCC'GGG
Cuts at: 1872 2236 4612 1872
Size: 364 2376 4545
Fragments arranged by size:
4545 2376 364
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2490 2592 3668 5389 6290 6552
Size: 197 926 547 102 1076 1721 901 262
Cuts at: 6552 820
Size: 1553
Fragments arranged by size:
1721 1553 1076 926 901 547 262 197
102
SnaBI TAC'GTA
Cuts at: 493 4582 5076 493
Size: 4089 494 2702
Fragments arranged by size:
4089 2702 494
SpeI A'CTAG_T
Cuts at: 152 4138 152
Size: 3986 3299
SphI G_CATG'C
Cuts at: 1132 4643 1132
Size: 3511 3774
SspI AAT'ATT
Cuts at: 5 52 5209 5
Size: 47 5157 2081
Fragments arranged by size:
5157 2081 47
StuI AGG'CCT
Cuts at: 1517 3765 1517
Size: 2248 5037
StyI C'CwwG_G
Cuts at: 513 1065 2310 3015 3244 4724 513
Size: 552 1245 705 229 1480 3074
Fragments arranged by size:
3074 1480 1245 705 552 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2951 3448 3893 5312 5329 5482 5667
Size: 1255 1371 497 445 1419 17 153 185
Cuts at: 5667 6112 325
Size: 445 1498
Fragments arranged by size:
1498 1419 1371 1255 497 445 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 5531 7243
Size: 274 80 33 51 1443 402 3152 1712
Cuts at: 7243 96
Size: 138
Fragments arranged by size:
3152 1712 1443 402 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3003 3035 3810 3842 7245 7277 3003
Size: 32 775 32 3403 32 3011
Fragments arranged by size:
3403 3011 775 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7285
XbaI T'CTAG_A
Cuts at: 4621 4621
Size: 7285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2956 4258 2956
Size: 1302 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7285
XmaI C'CCGG_G
Cuts at: 1870 2234 4610 1870
Size: 364 2376 4545
Fragments arranged by size:
4545 2376 364
XmnI GAAnn'nnTTC
Cuts at: 3543 5414 3543
Size: 1871 5414
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5093 276
Size: 53 83 186 936 3559 2468
Fragments arranged by size:
3559 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI EcoRV FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SanDI SfoI SrfI SwaI