Restriction map for
pFN21ASDA0588
(Circular) (Six-base) MAPSORT of: pFN21ASDA0588.seq Check: 6612 from: 1 to: 7525
pFN21ASDA0588 7525 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4763 4763
Size: 7525
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5335 278
Size: 53 83 186 936 3799 2468
Fragments arranged by size:
3799 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4707 4868 1937
Size: 2770 161 4594
Fragments arranged by size:
4594 2770 161
Acc65I G'GTAC_C
Cuts at: 4846 4846
Size: 7525
AclI AA'CG_TT
Cuts at: 1563 5652 6025 1563
Size: 4089 373 3063
Fragments arranged by size:
4089 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4188 5588 6972 7240 1632
Size: 2556 1400 1384 268 1917
Fragments arranged by size:
2556 1917 1400 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7328
AflIII A'CryG_T
Cuts at: 1203 3534 3657 6424 7401 1203
Size: 2331 123 2767 977 1327
Fragments arranged by size:
2767 2331 1327 977 123
AgeI A'CCGG_T
Cuts at: 6356 6356
Size: 7525
AhdI GACnn_n'nnGTC
Cuts at: 6254 6254
Size: 7525
AleI CACnn'nnGTG
Cuts at: 1166 3743 1166
Size: 2577 4948
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2819 1184
Size: 1635 5890
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2011 2043 2011
Size: 32 7493
AlwNI CAG_nnn'CTG
Cuts at: 2465 5268 6840 2465
Size: 2803 1572 3150
Fragments arranged by size:
3150 2803 1572
ApaLI G'TGCA_C
Cuts at: 5582 6738 5582
Size: 1156 6369
ApoI r'AATT_y
Cuts at: 1493 4834 5060 5212 7321 1493
Size: 3341 226 152 2109 1697
Fragments arranged by size:
3341 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3116 6079 160
Size: 872 2084 2963 1606
Fragments arranged by size:
2963 2084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7525
AvaI C'yCGr_G
Cuts at: 1870 1943 2190 2756 3701 4055 4850 1870
Size: 73 247 566 945 354 795 4545
Fragments arranged by size:
4545 945 795 566 354 247 73
BamHI G'GATC_C
Cuts at: 2497 4855 5222 2497
Size: 2358 367 4800
Fragments arranged by size:
4800 2358 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2693 3636 4496 4529 4628
Size: 325 217 627 906 943 860 33 99
Cuts at: 4628 4634 4846 6301 618
Size: 6 212 1455 1842
Fragments arranged by size:
1842 1455 943 906 860 627 325 217
212 99 33 6
BanII G_rGCy'C
Cuts at: 1391 2190 2199 2307 2538 3578 3774 3916 4392
Size: 799 9 108 231 1040 196 142 476
Cuts at: 4392 4613 4844 1391
Size: 221 231 4072
Fragments arranged by size:
4072 1040 799 476 231 231 221 196
142 108 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3367 4123 928
Size: 971 1468 756 4330
Fragments arranged by size:
4330 1468 971 756
BbvCI CC'TCA_GC
Cuts at: 3647 3647
Size: 7525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3507 3541 5716 5750 1903
Size: 34 1570 34 2175 34 3678
Fragments arranged by size:
3678 2175 1570 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5417 6627 5417
Size: 1210 6315
BclI T'GATC_A
Cuts at: 1548 3024 1548
Size: 1476 6049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6136 136
Size: 107 122 71 631 605 4464 1525
Fragments arranged by size:
4464 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7520 7520
Size: 7525
BlpI GC'TnA_GC
Cuts at: 2537 2751 3572 4942 2537
Size: 214 821 1370 5120
Fragments arranged by size:
5120 1370 821 214
Bme1580I G_kGCm'C
Cuts at: 1790 2798 3639 4689 5586 6742 1790
Size: 1008 841 1050 897 1156 2573
Fragments arranged by size:
2573 1156 1050 1008 897 841
BmgBI CAC'GTC
Cuts at: 1318 3040 1318
Size: 1722 5803
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4660 5256
Size: 416 53 83 399 24 2147 1096 596
Cuts at: 5256 6214 442
Size: 958 1753
Fragments arranged by size:
2147 1753 1096 958 596 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7525
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 3449 3950 2164
Size: 1285 501 5739
Fragments arranged by size:
5739 1285 501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2152 2159 2204 4047 6185 1239
Size: 913 7 45 1843 2138 2579
Fragments arranged by size:
2579 2138 1843 913 45 7
Bpu10I CC'TnA_GC
Cuts at: 3647 4163 3647
Size: 516 7009
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3833 3920 5650 6515 6813 3833
Size: 87 1730 865 298 4545
Fragments arranged by size:
4545 1730 865 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2090 4126 4483 6188 882
Size: 617 591 2036 357 1705 2219
Fragments arranged by size:
2219 2036 1705 617 591 357
BsaAI yAC'GTr
Cuts at: 493 1204 3535 4684 4822 5316 493
Size: 711 2331 1149 138 494 2702
Fragments arranged by size:
2702 2331 1149 711 494 138
BsaBI GATnn'nnATC
Cuts at: 7519 7519
Size: 7525
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4232 4583 5332 5714
Size: 53 83 186 936 2699 351 749 382
Cuts at: 5714 6349 275
Size: 635 1451
Fragments arranged by size:
2699 1451 936 749 635 382 351 186
83 53
BsaWI w'CCGG_w
Cuts at: 2099 2128 2500 2776 2865 5958 6356 6630 6777
Size: 29 372 276 89 3093 398 274 147
Cuts at: 6777 7188 2099
Size: 411 2436
Fragments arranged by size:
3093 2436 411 398 372 276 274 147
89 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2367 2397 3797 3827 4802
Size: 30 165 30 964 30 1400 30 975
Cuts at: 4802 4832 1178
Size: 30 3871
Fragments arranged by size:
3871 1400 975 964 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2315 2966 3438 1186
Size: 1129 651 472 5273
Fragments arranged by size:
5273 1129 651 472
BseYI C'CCAG_C
Cuts at: 2068 4335 4768 6728 7224 2068
Size: 2267 433 1960 496 2369
Fragments arranged by size:
2369 2267 1960 496 433
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2024 3869 4280 1163
Size: 573 288 1845 411 4408
Fragments arranged by size:
4408 1845 573 411 288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4975 5736 6764 1540
Size: 275 186 2974 761 1028 2301
Fragments arranged by size:
2974 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2199 2307 2538 3578 4613 5586 5671 6742 2199
Size: 108 231 1040 1035 973 85 1071 2982
Fragments arranged by size:
2982 1071 1040 1035 973 231 108 85
BsmI GAATG_Cn'
Cuts at: 5036 5129 5036
Size: 93 7432
BsmBI CGTCTCn'nnnn_
Cuts at: 3442 3921 3442
Size: 479 7046
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2199 2307 2538 2798 3578 3639
Size: 399 400 9 108 231 260 780 61
Cuts at: 3639 3774 3916 4392 4613 4689 4844 5586 5671
Size: 135 142 476 221 76 155 742 85
Cuts at: 5671 6742 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 780 742 476 400 399 260
231 221 155 142 135 108 85 76
61 9
BspEI T'CCGG_A
Cuts at: 2128 2500 2776 7188 2128
Size: 372 276 4412 2465
Fragments arranged by size:
4412 2465 372 276
BspHI T'CATG_A
Cuts at: 5414 7144 5414
Size: 1730 5795
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4763 4880 844
Size: 3919 117 3489
Fragments arranged by size:
3919 3489 117
BsrBI CCG'CTC
Cuts at: 1394 1823 3762 5412 1394
Size: 429 1939 1650 3507
Fragments arranged by size:
3507 1939 1650 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2991 4729 4786 6020 6194 65
Size: 1183 1743 1738 57 1234 174 1396
Fragments arranged by size:
1743 1738 1396 1234 1183 174 57
BsrFI r'CCGG_y
Cuts at: 1700 1810 3370 4228 4526 4631 6169 6356 1700
Size: 110 1560 858 298 105 1538 187 2869
Fragments arranged by size:
2869 1560 1538 858 298 187 110 105
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7525
BssHII G'CGCG_C
Cuts at: 1925 2699 1925
Size: 774 6751
BssSI C'ACGA_G
Cuts at: 2948 4286 5585 6597 2948
Size: 1338 1299 1012 3876
Fragments arranged by size:
3876 1338 1299 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4684 794
Size: 3890 3635
BstBI TT'CG_AA
Cuts at: 2007 6329 2007
Size: 4322 3203
BstEII G'GTnAC_C
Cuts at: 1184 3784 1184
Size: 2600 4925
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 3745 3796 4579 2176
Size: 1569 51 783 5122
Fragments arranged by size:
5122 1569 783 51
BstYI r'GATC_y
Cuts at: 1972 2497 3705 4855 5222 5607 5624 7076 7162
Size: 525 1208 1150 367 385 17 1452 86
Cuts at: 7162 7174 7520 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1208 1150 525 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3381 3381
Size: 7525
BtgI C'CryG_G
Cuts at: 513 1065 3321 3726 3967 4005 4011 4116 4302
Size: 552 2256 405 241 38 6 105 186
Cuts at: 4302 4638 513
Size: 336 3400
Fragments arranged by size:
3400 2256 552 405 336 241 186 105
38 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4533 487
Size: 683 56 3307 3479
Fragments arranged by size:
3479 3307 683 56
BtsI GCAGTG_nn'
Cuts at: 5041 5835 5855 5041
Size: 794 20 6711
Fragments arranged by size:
6711 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7525
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7490
DraI TTT'AAA
Cuts at: 3129 3199 4829 3129
Size: 70 1630 5825
Fragments arranged by size:
5825 1630 70
DraIII CAC_nnn'GTG
Cuts at: 4243 4243
Size: 7525
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2837 4358 6532 809
Size: 507 1521 1521 2174 1802
Fragments arranged by size:
2174 1802 1521 1521 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3817 3954 3970
Size: 54 1425 50 275 18 1987 137 16
Cuts at: 3970 4008 4014 4113 4972 5861 7490 8
Size: 38 6 99 859 889 1629 43
Fragments arranged by size:
1987 1629 1425 889 859 275 137 99
54 50 43 38 18 16 6
EagI C'GGCC_G
Cuts at: 1537 1812 4972 1537
Size: 275 3160 4090
Fragments arranged by size:
4090 3160 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 3462 3875 4310 5455 1353
Size: 774 1335 413 435 1145 3423
Fragments arranged by size:
3423 1335 1145 774 435 413
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3976 4055 4576 6112 6486 6632
Size: 1064 138 2387 79 521 1536 374 146
Cuts at: 6632 387
Size: 1280
Fragments arranged by size:
2387 1536 1280 1064 521 374 146 138
79
EcoICRI GAG'CTC
Cuts at: 2197 2305 2536 3576 4611 2197
Size: 108 231 1040 1035 5111
Fragments arranged by size:
5111 1040 1035 231 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 2159 2204 4047 4188 5588 6185
Size: 393 520 7 45 1843 141 1400 597
Cuts at: 6185 6972 7240 1239
Size: 787 268 1524
Fragments arranged by size:
1843 1524 1400 787 597 520 393 268
141 45 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2832 4196 2832
Size: 1364 6161
EcoO109I rG'GnC_Cy
Cuts at: 2173 4765 2173
Size: 2592 4933
EcoRI G'AATT_C
Cuts at: 4834 5212 4834
Size: 378 7147
EcoRV GAT'ATC
Cuts at: 2506 2506
Size: 7525
FspI TGC'GCA
Cuts at: 2687 3902 6031 2687
Size: 1215 2129 4181
Fragments arranged by size:
4181 2129 1215
FspAI rTGC'GCAy
Cuts at: 2687 3902 2687
Size: 1215 6310
HaeII r_GCGC'y
Cuts at: 1678 4163 4177 4229 4653 6672 1678
Size: 2485 14 52 424 2019 2531
Fragments arranged by size:
2531 2485 2019 424 52 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2202 2234 3003 3035 3153
Size: 32 135 32 630 32 769 32 118
Cuts at: 3153 3185 3797 3829 4595 4627 6166 6198 6240
Size: 32 612 32 766 32 1539 32 42
Cuts at: 6240 6272 1373
Size: 32 2626
Fragments arranged by size:
2626 1539 769 766 630 612 135 118
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2815 4869 5115 669
Size: 988 281 877 2054 246 3079
Fragments arranged by size:
3079 2054 988 877 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7525
HpaI GTT'AAC
Cuts at: 5115 5115
Size: 7525
KpnI G_GTAC'C
Cuts at: 4850 4850
Size: 7525
MfeI C'AATT_G
Cuts at: 5124 5124
Size: 7525
MluI A'CGCG_T
Cuts at: 7401 7401
Size: 7525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2691 2922 3573 6639 6823 1305
Size: 1386 231 651 3066 184 2007
Fragments arranged by size:
3066 2007 1386 651 231 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5703
Fragments arranged by size:
5703 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 2468 2708 3284 3743 4559 5483
Size: 648 1182 120 240 576 459 816 924
Cuts at: 5483 5842 6001 518
Size: 359 159 2042
Fragments arranged by size:
2042 1182 924 816 648 576 459 359
240 159 120
MspA1I CmG'CkG
Cuts at: 1606 2265 2803 4118 4503 4745 4941 5618 6766
Size: 659 538 1315 385 242 196 677 1148
Cuts at: 6766 7011 1606
Size: 245 2120
Fragments arranged by size:
2120 1315 1148 677 659 538 385 245
242 196
NaeI GCC'GGC
Cuts at: 4230 4633 4230
Size: 403 7122
NcoI C'CATG_G
Cuts at: 513 1065 4638 513
Size: 552 3573 3400
Fragments arranged by size:
3573 3400 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7525
NgoMIV G'CCGG_C
Cuts at: 4228 4631 4228
Size: 403 7122
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7525
NotI GC'GGCC_GC
Cuts at: 4972 4972
Size: 7525
NspI r_CATG'y
Cuts at: 1132 1229 4357 4883 6428 1132
Size: 97 3128 526 1545 2229
Fragments arranged by size:
3128 2229 1545 526 97
PasI CC'CwG_GG
Cuts at: 1348 4618 1348
Size: 3270 4255
PciI A'CATG_T
Cuts at: 6424 6424
Size: 7525
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4036 4775 1108
Size: 2928 739 3858
Fragments arranged by size:
3858 2928 739
PfoI T'CCnGG_A
Cuts at: 3960 3960
Size: 7525
PmeI GTTT'AAAC
Cuts at: 4829 4829
Size: 7525
PmlI CAC'GTG
Cuts at: 4684 4684
Size: 7525
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2459 2491 5594 5626 7107 7139 2459
Size: 32 3103 32 1481 32 2845
Fragments arranged by size:
3103 2845 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2173 2173
Size: 7525
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7525
PsiI TTA'TAA
Cuts at: 5095 5095
Size: 7525
PspXI vC'TCGA_Gb
Cuts at: 4055 4055
Size: 7525
PstI C_TGCA'G
Cuts at: 830 2526 4200 4275 4680 4877 830
Size: 1696 1674 75 405 197 3478
Fragments arranged by size:
3478 1696 1674 405 197 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7525
PvuII CAG'CTG
Cuts at: 2803 4745 2803
Size: 1942 5583
SacI G_AGCT'C
Cuts at: 2199 2307 2538 3578 4613 2199
Size: 108 231 1040 1035 5111
Fragments arranged by size:
5111 1040 1035 231 108
SacII CC_GC'GG
Cuts at: 4119 4119
Size: 7525
SalI G'TCGA_C
Cuts at: 1936 4867 1936
Size: 2931 4594
SapI GCTCTTCn'nnn_
Cuts at: 2127 2127
Size: 7525
SbfI CC_TGCA'GG
Cuts at: 4877 4877
Size: 7525
ScaI AGT'ACT
Cuts at: 5773 7485 5773
Size: 1712 5813
SexAI A'CCwGG_T
Cuts at: 2649 3780 4098 2649
Size: 1131 318 6076
Fragments arranged by size:
6076 1131 318
SfcI C'TryA_G
Cuts at: 826 1045 2522 3226 4196 4271 4676 4873 6008
Size: 219 1477 704 970 75 405 197 1135
Cuts at: 6008 6689 6880 826
Size: 681 191 1471
Fragments arranged by size:
1477 1471 1135 970 704 681 405 219
197 191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7525
SgrAI Cr'CCGG_yG
Cuts at: 1700 4228 1700
Size: 2528 4997
SmaI CCC'GGG
Cuts at: 1872 2192 4852 1872
Size: 320 2660 4545
Fragments arranged by size:
4545 2660 320
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3848 3935 4055 5629 6530 6792
Size: 197 926 1905 87 120 1574 901 262
Cuts at: 6792 820
Size: 1553
Fragments arranged by size:
1905 1574 1553 926 901 262 197 120
87
SnaBI TAC'GTA
Cuts at: 493 4822 5316 493
Size: 4329 494 2702
Fragments arranged by size:
4329 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7525
SphI G_CATG'C
Cuts at: 1132 4883 1132
Size: 3751 3774
SspI AAT'ATT
Cuts at: 5 52 5449 5
Size: 47 5397 2081
Fragments arranged by size:
5397 2081 47
StuI AGG'CCT
Cuts at: 1517 3386 1517
Size: 1869 5656
StyI C'CwwG_G
Cuts at: 513 1065 2790 3181 3511 3789 4638 4964 513
Size: 552 1725 391 330 278 849 326 3074
Fragments arranged by size:
3074 1725 849 552 391 330 326 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 5552 5569 5722 5907 6352 325
Size: 1255 543 3429 17 153 185 445 1498
Fragments arranged by size:
3429 1498 1255 543 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5771 7483 96
Size: 274 80 33 51 1443 3794 1712 138
Fragments arranged by size:
3794 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4277 4309 7485 7517 4277
Size: 32 3176 32 4285
Fragments arranged by size:
4285 3176 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3245 1316
Size: 1929 5596
XbaI T'CTAG_A
Cuts at: 2338 4861 2338
Size: 2523 5002
XhoI C'TCGA_G
Cuts at: 1943 4055 1943
Size: 2112 5413
XmaI C'CCGG_G
Cuts at: 1870 2190 4850 1870
Size: 320 2660 4545
Fragments arranged by size:
4545 2660 320
XmnI GAAnn'nnTTC
Cuts at: 3264 5654 3264
Size: 2390 5135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5333 276
Size: 53 83 186 936 3799 2468
Fragments arranged by size:
3799 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I FalI FseI KasI NarI NruI NsiI PacI
PspOMI PsrI RsrII SanDI SfiI SfoI SrfI SwaI
XcmI