Restriction map for
pFN21ASDA0573
(Circular) (Six-base) MAPSORT of: pFN21ASDA0573.seq Check: 4439 from: 1 to: 5947
pFN21ASDA0573 5947 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3212 3290 1937
Size: 1275 78 4594
Fragments arranged by size:
4594 1275 78
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4010 5394 5662 1632
Size: 2378 1384 268 1917
Fragments arranged by size:
2378 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 4846 5823 1203
Size: 3643 977 1327
Fragments arranged by size:
3643 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5947
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2011 2043 2011
Size: 32 5915
AlwNI CAG_nnn'CTG
Cuts at: 3690 5262 3690
Size: 1572 4375
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 2145 2176 2255 2460 2707 2930 3098 3256
Size: 652 31 79 205 247 223 168 158
Cuts at: 3256 3482 3634 5743 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 652 247 226 223 205 168
158 152 79 31
AseI AT'TA_AT
Cuts at: 160 1032 2882 3038 4501 160
Size: 872 1850 156 1463 1606
Fragments arranged by size:
1850 1606 1463 872 156
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3268 4723 618
Size: 325 217 627 1481 1455 1842
Fragments arranged by size:
1842 1481 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3179 3266 1391
Size: 1788 87 4072
Fragments arranged by size:
4072 1788 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2029 928
Size: 971 130 4846
Fragments arranged by size:
4846 971 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2557 2591 4138 4172 1903
Size: 34 620 34 1547 34 3678
Fragments arranged by size:
3678 1547 620 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 2299 1548
Size: 751 5196
BfrBI ATG'CAT
Cuts at: 2026 2026
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4558 136
Size: 107 122 71 631 605 2886 1525
Fragments arranged by size:
2886 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 4008 5164 1790
Size: 2218 1156 2573
Fragments arranged by size:
2573 2218 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3192 3678 4636
Size: 416 53 83 399 24 1775 486 958
Cuts at: 4636 442
Size: 1753
Fragments arranged by size:
1775 1753 958 486 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2949 4607 1239
Size: 1710 1658 2579
Fragments arranged by size:
2579 1710 1658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3038 4072 4937 5235 3038
Size: 1034 865 298 3750
Fragments arranged by size:
3750 1034 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4610 882
Size: 617 3111 2219
Fragments arranged by size:
3111 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3244 3738 493
Size: 711 2040 494 2702
Fragments arranged by size:
2702 2040 711 494
BsaBI GATnn'nnATC
Cuts at: 2298 5941 2298
Size: 3643 2304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3754 4136 4771 275
Size: 53 83 186 936 2221 382 635 1451
Fragments arranged by size:
2221 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2623 2677 4380 4778 5052 5199 5610 2623
Size: 54 1703 398 274 147 411 2960
Fragments arranged by size:
2960 1703 411 398 274 147 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5722
Fragments arranged by size:
5722 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5947
BseYI C'CCAG_C
Cuts at: 5150 5646 5150
Size: 496 5451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2933 3074 1163
Size: 573 1197 141 4036
Fragments arranged by size:
4036 1197 573 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3179 4008 4093 5164 3179
Size: 829 85 1071 3962
Fragments arranged by size:
3962 1071 829 85
BsmI GAATG_Cn'
Cuts at: 2668 3458 3551 2668
Size: 790 93 5064
Fragments arranged by size:
5064 790 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3179 3266 4008 4093 5164 1391
Size: 399 1389 87 742 85 1071 2174
Fragments arranged by size:
2174 1389 1071 742 399 87 85
BspEI T'CCGG_A
Cuts at: 2677 5610 2677
Size: 2933 3014
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3302 844
Size: 2458 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3834 1394
Size: 429 2011 3507
Fragments arranged by size:
3507 2011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4442 4616 65
Size: 1183 3194 174 1396
Fragments arranged by size:
3194 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4591 4778 1700
Size: 110 2781 187 2869
Fragments arranged by size:
2869 2781 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5947
BstBI TT'CG_AA
Cuts at: 2007 4751 2007
Size: 2744 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942
Size: 1305 367 385 17 1452 86 12 346
Cuts at: 5942 1972
Size: 1977
Fragments arranged by size:
1977 1452 1305 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3213 3213
Size: 5947
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5395
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5208
Fragments arranged by size:
5208 683 56
BtsI GCAGTG_nn'
Cuts at: 3463 4257 4277 3463
Size: 794 20 5133
Fragments arranged by size:
5133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 2151 3251 2151
Size: 1100 4847
DraIII CAC_nnn'GTG
Cuts at: 3200 3200
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3394 4283 5912
Size: 54 1425 50 275 18 1564 889 1629
Cuts at: 5912 8
Size: 43
Fragments arranged by size:
1629 1564 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2375 2949 3877 1353
Size: 1022 574 928 3423
Fragments arranged by size:
3423 1022 928 574
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3177 3177
Size: 5947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2949 4010 4607 5394 5662 1239
Size: 393 1317 1061 597 787 268 1524
Fragments arranged by size:
1524 1317 1061 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3098 3256 3634 3098
Size: 158 378 5411
Fragments arranged by size:
5411 378 158
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 5094 1678
Size: 3416 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2200 2232 2291 2323 4588
Size: 32 135 32 628 32 59 32 2265
Cuts at: 4588 4620 4662 4694 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2265 628 135 59 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3291 3537 669
Size: 988 281 1353 246 3079
Fragments arranged by size:
3079 1353 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2232 748
Size: 1484 4463
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 2725 3546 2725
Size: 821 5126
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5061 5245 1305
Size: 3756 184 2007
Fragments arranged by size:
3756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4125
Fragments arranged by size:
4125 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3009 3905 4264 4423 518
Size: 648 1843 896 359 159 2042
Fragments arranged by size:
2042 1843 896 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3363 4040 5188 5433 1606
Size: 1757 677 1148 245 2120
Fragments arranged by size:
2120 1757 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5395
NdeI CA'TA_TG
Cuts at: 387 3006 387
Size: 2619 3328
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NsiI A_TGCA'T
Cuts at: 2028 2028
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 2918 3305 4850 1132
Size: 97 1689 387 1545 2229
Fragments arranged by size:
2229 1689 1545 387 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5947
PfoI T'CCnGG_A
Cuts at: 3043 3043
Size: 5947
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2193 1613
Size: 580 5367
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PstI C_TGCA'G
Cuts at: 830 3299 830
Size: 2469 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
SacI G_AGCT'C
Cuts at: 3179 3179
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 2545 3295 4430 5111 5302 826
Size: 219 1500 750 1135 681 191 1471
Fragments arranged by size:
1500 1471 1135 750 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3053 4051 4952 5214 820
Size: 197 926 1110 998 901 262 1553
Fragments arranged by size:
1553 1110 998 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3244 3738 493
Size: 2751 494 2702
Fragments arranged by size:
2751 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SspI AAT'ATT
Cuts at: 5 52 2572 2856 3871 5
Size: 47 2520 284 1015 2081
Fragments arranged by size:
2520 2081 1015 284 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5947
StyI C'CwwG_G
Cuts at: 513 1065 3386 513
Size: 552 2321 3074
Fragments arranged by size:
3074 2321 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3211 3974 3991 4144 4329 4774 325
Size: 1255 1631 763 17 153 185 445 1498
Fragments arranged by size:
1631 1498 1255 763 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2688 4193 5905
Size: 274 80 33 51 1443 711 1505 1712
Cuts at: 5905 96
Size: 138
Fragments arranged by size:
1712 1505 1443 711 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5907 5939 5907
Size: 32 5915
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 2256 4076 2256
Size: 1820 4127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI