Restriction map for
pFN21ASDA0557
(Circular) (Six-base) MAPSORT of: pFN21ASDA0557.seq Check: 1589 from: 1 to: 6169
pFN21ASDA0557 6169 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3008 3344 3008
Size: 336 5833
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3035 3979 278
Size: 53 83 186 936 1499 944 2468
Fragments arranged by size:
2468 1499 944 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2715 3512 1937
Size: 778 797 4594
Fragments arranged by size:
4594 797 778
Acc65I G'GTAC_C
Cuts at: 2847 3490 2847
Size: 643 5526
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3007 3109 3175 3277 4232 5616 5884 1632
Size: 1375 102 66 102 955 1384 268 1917
Fragments arranged by size:
1917 1384 1375 955 268 102 102 66
AfeI AGC'GCT
Cuts at: 3188 3272 3188
Size: 84 6085
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5972
AflIII A'CryG_T
Cuts at: 1203 5068 6045 1203
Size: 3865 977 1327
Fragments arranged by size:
3865 1327 977
AgeI A'CCGG_T
Cuts at: 5000 5000
Size: 6169
AhdI GACnn_n'nnGTC
Cuts at: 4898 4898
Size: 6169
AleI CACnn'nnGTG
Cuts at: 1166 3318 1166
Size: 2152 4017
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3392 1184
Size: 2208 3961
AlwNI CAG_nnn'CTG
Cuts at: 2050 2071 2290 2317 3912 5484 2050
Size: 21 219 27 1595 1572 2735
Fragments arranged by size:
2735 1595 1572 219 27 21
ApaI G_GGCC'C
Cuts at: 2214 2600 2214
Size: 386 5783
ApaLI G'TGCA_C
Cuts at: 3143 4226 5382 3143
Size: 1083 1156 3930
Fragments arranged by size:
3930 1156 1083
ApoI r'AATT_y
Cuts at: 1493 3478 3704 3856 5965 1493
Size: 1985 226 152 2109 1697
Fragments arranged by size:
2109 1985 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4723 160
Size: 872 3691 1606
Fragments arranged by size:
3691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2346 2471 3173 3494 1870
Size: 73 271 132 125 702 321 4545
Fragments arranged by size:
4545 702 321 271 132 125 73
BamHI G'GATC_C
Cuts at: 2790 3499 3866 2790
Size: 709 367 5093
Fragments arranged by size:
5093 709 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2464 2686 2847 3368 3490
Size: 325 217 627 677 222 161 521 122
Cuts at: 3490 4945 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 677 627 521 325 222 217
161 122
BanII G_rGCy'C
Cuts at: 1391 2133 2214 2441 2600 3203 3488 1391
Size: 742 81 227 159 603 285 4072
Fragments arranged by size:
4072 742 603 285 227 159 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2971 928
Size: 971 1072 4126
Fragments arranged by size:
4126 1072 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4360 4394 1903
Size: 34 2423 34 3678
Fragments arranged by size:
3678 2423 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4061 5271 4061
Size: 1210 4959
BclI T'GATC_A
Cuts at: 1548 2935 1548
Size: 1387 4782
BfrBI ATG'CAT
Cuts at: 2742 2742
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4780 136
Size: 107 122 71 631 605 3108 1525
Fragments arranged by size:
3108 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6164 6164
Size: 6169
BlpI GC'TnA_GC
Cuts at: 2511 2576 2754 3586 2511
Size: 65 178 832 5094
Fragments arranged by size:
5094 832 178 65
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2469 2600 2691 3147 3173 3371 4230
Size: 424 255 131 91 456 26 198 859
Cuts at: 4230 5386 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 859 456 424 255 198 131
91 26
BmgBI CAC'GTC
Cuts at: 1318 2707 1318
Size: 1389 4780
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2906 3253 3900
Size: 416 53 83 399 24 1489 347 647
Cuts at: 3900 4858 442
Size: 958 1753
Fragments arranged by size:
1753 1489 958 647 416 399 347 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2640 2149
Size: 491 5678
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2027 2312 2327 2536 2552 2610 2792 3187
Size: 788 285 15 209 16 58 182 395
Cuts at: 3187 3410 3462 4829 1239
Size: 223 52 1367 2579
Fragments arranged by size:
2579 1367 788 395 285 223 209 182
58 52 16 15
Bpu10I CC'TnA_GC
Cuts at: 2225 2225
Size: 6169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4294 5159 5457 4294
Size: 865 298 5006
Fragments arranged by size:
5006 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4832 882
Size: 617 3333 2219
Fragments arranged by size:
3333 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3466 3960 493
Size: 711 2262 494 2702
Fragments arranged by size:
2702 2262 711 494
BsaBI GATnn'nnATC
Cuts at: 6163 6163
Size: 6169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2651 3032 3976 4358
Size: 53 83 186 936 1118 381 944 382
Cuts at: 4358 4993 275
Size: 635 1451
Fragments arranged by size:
1451 1118 944 936 635 382 381 186
83 53
BsaWI w'CCGG_w
Cuts at: 4602 5000 5274 5421 5832 4602
Size: 398 274 147 411 4939
Fragments arranged by size:
4939 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2636 2666 1178
Size: 30 165 30 1233 30 4681
Fragments arranged by size:
4681 1233 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2141 2393 2744 1186
Size: 934 21 252 351 4611
Fragments arranged by size:
4611 934 351 252 21
BseYI C'CCAG_C
Cuts at: 2153 2207 2566 2571 2749 5372 5868 2153
Size: 54 359 5 178 2623 496 2454
Fragments arranged by size:
2623 2454 496 359 178 54 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3238 3362 1163
Size: 573 1502 124 3970
Fragments arranged by size:
3970 1502 573 124
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2264 3115 3619 4380 5408 1540
Size: 275 186 263 851 504 761 1028 2301
Fragments arranged by size:
2301 1028 851 761 504 275 263 186
BsiHKAI G_wGCw'C
Cuts at: 2859 3147 3203 4230 4315 5386 2859
Size: 288 56 1027 85 1071 3642
Fragments arranged by size:
3642 1071 1027 288 85 56
BsmI GAATG_Cn'
Cuts at: 3680 3773 3680
Size: 93 6076
BsmBI CGTCTCn'nnnn_
Cuts at: 2135 2135
Size: 6169
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2214 2441 2469 2600 2691 2859
Size: 399 343 81 227 28 131 91 168
Cuts at: 2859 3147 3173 3203 3371 3488 4230 4315 5386
Size: 288 26 30 168 117 742 85 1071
Cuts at: 5386 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 343 288 227 168
168 131 117 91 85 81 30 28
26
BspEI T'CCGG_A
Cuts at: 5832 5832
Size: 6169
BspHI T'CATG_A
Cuts at: 4058 5788 4058
Size: 1730 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 3344 3384 3524 844
Size: 2164 336 40 140 3489
Fragments arranged by size:
3489 2164 336 140 40
BsrBI CCG'CTC
Cuts at: 1394 1823 2373 2890 3073 3117 3241 4056 1394
Size: 429 550 517 183 44 124 815 3507
Fragments arranged by size:
3507 815 550 517 429 183 124 44
BsrDI GCAATG_nn'
Cuts at: 65 1248 4664 4838 65
Size: 1183 3416 174 1396
Fragments arranged by size:
3416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3430 4813 5000 1700
Size: 110 1620 1383 187 2869
Fragments arranged by size:
2869 1620 1383 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6169
BssSI C'ACGA_G
Cuts at: 2393 4229 5241 2393
Size: 1836 1012 3321
Fragments arranged by size:
3321 1836 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3261 794
Size: 2467 3702
BstBI TT'CG_AA
Cuts at: 2007 4973 2007
Size: 2966 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6169
BstXI CCAn_nnnn'nTGG
Cuts at: 2879 2879
Size: 6169
BstYI r'GATC_y
Cuts at: 1972 2085 2286 2337 2521 2790 3499 3866 4251
Size: 113 201 51 184 269 709 367 385
Cuts at: 4251 4268 5720 5806 5818 6164 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 709 385 367 346 269 201
184 113 86 51 17 12
BstZ17I GTA'TAC
Cuts at: 2716 2716
Size: 6169
BtgI C'CryG_G
Cuts at: 513 1065 2022 2136 2385 2977 513
Size: 552 957 114 249 592 3705
Fragments arranged by size:
3705 957 592 552 249 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5430
Fragments arranged by size:
5430 683 56
BtsI GCAGTG_nn'
Cuts at: 3685 4479 4499 3685
Size: 794 20 5355
Fragments arranged by size:
5355 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6134
DraI TTT'AAA
Cuts at: 3473 3473
Size: 6169
DraIII CAC_nnn'GTG
Cuts at: 3261 3261
Size: 6169
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5176 809
Size: 507 3860 1802
Fragments arranged by size:
3860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2261 2609
Size: 54 1425 50 275 18 195 236 348
Cuts at: 2609 2881 3349 3616 4505 6134 8
Size: 272 468 267 889 1629 43
Fragments arranged by size:
1629 1425 889 468 348 275 272 267
236 195 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2261 3616 1537
Size: 275 449 1355 4090
Fragments arranged by size:
4090 1355 449 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2118 2183 2192 2532 4099 1353
Size: 723 42 65 9 340 1567 3423
Fragments arranged by size:
3423 1567 723 340 65 42 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2159 3350 4756 5130 5276 387
Size: 1064 138 570 1191 1406 374 146 1280
Fragments arranged by size:
1406 1280 1191 1064 570 374 146 138
EcoICRI GAG'CTC
Cuts at: 3201 3201
Size: 6169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 2312 2327 2536 2552 2610 2792
Size: 393 395 285 15 209 16 58 182
Cuts at: 2792 3007 3109 3175 3187 3277 3410 3462 4232
Size: 215 102 66 12 90 133 52 770
Cuts at: 4232 4829 5616 5884 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 770 597 395 393 285 268
215 209 182 133 102 90 66 58
52 16 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2075 2075
Size: 6169
EcoO109I rG'GnC_Cy
Cuts at: 2068 2149 2211 2221 2596 2621 2783 2876 3446
Size: 81 62 10 375 25 162 93 570
Cuts at: 3446 2068
Size: 4791
Fragments arranged by size:
4791 570 375 162 93 81 62 25
10
EcoRI G'AATT_C
Cuts at: 3478 3856 3478
Size: 378 5791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2988 2988
Size: 6169
FspI TGC'GCA
Cuts at: 2273 4675 2273
Size: 2402 3767
HaeII r_GCGC'y
Cuts at: 1678 3190 3274 3300 5316 1678
Size: 1512 84 26 2016 2531
Fragments arranged by size:
2531 2016 1512 84 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2329 2361 2510 2542 2636
Size: 32 135 32 757 32 149 32 94
Cuts at: 2636 2668 2775 2807 4810 4842 4884 4916 1373
Size: 32 107 32 2003 32 42 32 2626
Fragments arranged by size:
2626 2003 757 149 135 107 94 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3513 3759 669
Size: 988 281 1575 246 3079
Fragments arranged by size:
3079 1575 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KpnI G_GTAC'C
Cuts at: 2851 3494 2851
Size: 643 5526
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2778 3144 5283 5467 1305
Size: 1473 366 2139 184 2007
Fragments arranged by size:
2139 2007 1473 366 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 2611 2883 10
Size: 54 1425 343 195 584 272 3296
Fragments arranged by size:
3296 1425 584 343 272 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3318 4127 4486 4645 518
Size: 648 2152 809 359 159 2042
Fragments arranged by size:
2152 2042 809 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2253 2410 2434 2575 2602 2758 2770 3234
Size: 647 157 24 141 27 156 12 464
Cuts at: 3234 3391 3585 4262 5410 5655 1606
Size: 157 194 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 647 464 245 194 157
157 156 141 27 24 12
NcoI C'CATG_G
Cuts at: 513 1065 2022 2977 513
Size: 552 957 955 3705
Fragments arranged by size:
3705 957 955 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NsiI A_TGCA'T
Cuts at: 2744 2744
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 3527 5072 1132
Size: 97 2298 1545 2229
Fragments arranged by size:
2298 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6169
PciI A'CATG_T
Cuts at: 5068 5068
Size: 6169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2203 3433 1108
Size: 1095 1230 3844
Fragments arranged by size:
3844 1230 1095
PfoI T'CCnGG_A
Cuts at: 2289 3439 2289
Size: 1150 5019
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 5751 5783 4238
Size: 32 1481 32 4624
Fragments arranged by size:
4624 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 2149 2783 2068
Size: 81 634 5454
Fragments arranged by size:
5454 634 81
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6169
PsiI TTA'TAA
Cuts at: 3739 3739
Size: 6169
PspOMI G'GGCC_C
Cuts at: 2210 2596 2210
Size: 386 5783
PstI C_TGCA'G
Cuts at: 830 2409 3521 830
Size: 1579 1112 3478
Fragments arranged by size:
3478 1579 1112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
PvuII CAG'CTG
Cuts at: 2575 2575
Size: 6169
SacI G_AGCT'C
Cuts at: 3203 3203
Size: 6169
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SapI GCTCTTCn'nnn_
Cuts at: 2183 2192 2183
Size: 9 6160
SbfI CC_TGCA'GG
Cuts at: 3521 3521
Size: 6169
ScaI AGT'ACT
Cuts at: 4417 6129 4417
Size: 1712 4457
SfcI C'TryA_G
Cuts at: 826 1045 2405 3517 4652 5333 5524 826
Size: 219 1360 1112 1135 681 191 1471
Fragments arranged by size:
1471 1360 1135 1112 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6169
SmaI CCC'GGG
Cuts at: 1872 2216 3496 1872
Size: 344 1280 4545
Fragments arranged by size:
4545 1280 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4273 5174 5436 820
Size: 197 926 2330 901 262 1553
Fragments arranged by size:
2330 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3466 3960 493
Size: 2973 494 2702
Fragments arranged by size:
2973 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 3527 1132
Size: 2395 3774
SspI AAT'ATT
Cuts at: 5 52 4093 5
Size: 47 4041 2081
Fragments arranged by size:
4041 2081 47
StuI AGG'CCT
Cuts at: 1517 2932 1517
Size: 1415 4754
StyI C'CwwG_G
Cuts at: 513 1065 2022 2058 2277 2977 3608 513
Size: 552 957 36 219 700 631 3074
Fragments arranged by size:
3074 957 700 631 552 219 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3272 4196 4213 4366 4551 4996 325
Size: 1255 1692 924 17 153 185 445 1498
Fragments arranged by size:
1692 1498 1255 924 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4415 6127 96
Size: 274 80 33 51 1443 2438 1712 138
Fragments arranged by size:
2438 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6129 6161 6129
Size: 32 6137
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6169
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2204 2204
Size: 6169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 2214 3494 1870
Size: 344 1280 4545
Fragments arranged by size:
4545 1280 344
XmnI GAAnn'nnTTC
Cuts at: 3188 3272 4298 3188
Size: 84 1026 5059
Fragments arranged by size:
5059 1026 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3033 3977 276
Size: 53 83 186 936 1499 944 2468
Fragments arranged by size:
2468 1499 944 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BsiWI Bsu36I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI