Restriction map for
pFN21ASDA0554
(Circular) (Six-base) MAPSORT of: pFN21ASDA0554.seq Check: 4978 from: 1 to: 6580
pFN21ASDA0554 6580 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2731 2731
Size: 6580
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4390 278
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2222 3345 3923 1937
Size: 285 1123 578 4594
Fragments arranged by size:
4594 1123 578 285
Acc65I G'GTAC_C
Cuts at: 3901 3901
Size: 6580
AclI AA'CG_TT
Cuts at: 1563 4707 5080 1563
Size: 3144 373 3063
Fragments arranged by size:
3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3157 4643 6027 6295 1632
Size: 1525 1486 1384 268 1917
Fragments arranged by size:
1917 1525 1486 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6383
AflIII A'CryG_T
Cuts at: 1203 5479 6456 1203
Size: 4276 977 1327
Fragments arranged by size:
4276 1327 977
AgeI A'CCGG_T
Cuts at: 5411 5411
Size: 6580
AhdI GACnn_n'nnGTC
Cuts at: 5309 5309
Size: 6580
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6580
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6580
AlwNI CAG_nnn'CTG
Cuts at: 2347 4323 5895 2347
Size: 1976 1572 3032
Fragments arranged by size:
3032 1976 1572
ApaLI G'TGCA_C
Cuts at: 4637 5793 4637
Size: 1156 5424
ApoI r'AATT_y
Cuts at: 1493 2609 2870 2926 2957 3294 3443 3889 4115
Size: 1116 261 56 31 337 149 446 226
Cuts at: 4115 4267 6376 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1116 446 337 261 226 152
149 56 31
AscI GG'CGCG_CC
Cuts at: 3500 3500
Size: 6580
AseI AT'TA_AT
Cuts at: 160 1032 5134 160
Size: 872 4102 1606
Fragments arranged by size:
4102 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
AvaI C'yCGr_G
Cuts at: 1870 1943 3905 1870
Size: 73 1962 4545
Fragments arranged by size:
4545 1962 73
BamHI G'GATC_C
Cuts at: 3910 4277 3910
Size: 367 6213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 3901 5356 618
Size: 325 217 627 246 1868 1455 1842
Fragments arranged by size:
1868 1842 1455 627 325 246 217
BanII G_rGCy'C
Cuts at: 1391 2043 3575 3666 3899 1391
Size: 652 1532 91 233 4072
Fragments arranged by size:
4072 1532 652 233 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2723 3774 928
Size: 971 824 1051 3734
Fragments arranged by size:
3734 1051 971 824
BbvCI CC'TCA_GC
Cuts at: 3462 3462
Size: 6580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4771 4805 1903
Size: 34 2834 34 3678
Fragments arranged by size:
3678 2834 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4472 5682 4472
Size: 1210 5370
BclI T'GATC_A
Cuts at: 1548 2800 1548
Size: 1252 5328
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3458 3579 5191
Size: 107 122 71 631 605 1786 121 1612
Cuts at: 5191 136
Size: 1525
Fragments arranged by size:
1786 1612 1525 631 605 122 121 107
71
BglII A'GATC_T
Cuts at: 6575 6575
Size: 6580
BlpI GC'TnA_GC
Cuts at: 3997 3997
Size: 6580
Bme1580I G_kGCm'C
Cuts at: 1790 2036 4641 5797 1790
Size: 246 2605 1156 2573
Fragments arranged by size:
2605 2573 1156 246
BmgBI CAC'GTC
Cuts at: 1318 3684 1318
Size: 2366 4214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3088 4311 5269
Size: 416 53 83 399 24 1671 1223 958
Cuts at: 5269 442
Size: 1753
Fragments arranged by size:
1753 1671 1223 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2588 2880 5240 1239
Size: 1349 292 2360 2579
Fragments arranged by size:
2579 2360 1349 292
Bpu10I CC'TnA_GC
Cuts at: 3462 3462
Size: 6580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 3185 4705 5570 5868 2117
Size: 1068 1520 865 298 2829
Fragments arranged by size:
2829 1520 1068 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3363 3428 5243 882
Size: 617 1864 65 1815 2219
Fragments arranged by size:
2219 1864 1815 617 65
BsaAI yAC'GTr
Cuts at: 493 1204 3877 4371 493
Size: 711 2673 494 2702
Fragments arranged by size:
2702 2673 711 494
BsaBI GATnn'nnATC
Cuts at: 2750 6574 2750
Size: 3824 2756
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4387 4769 5404 275
Size: 53 83 186 936 2854 382 635 1451
Fragments arranged by size:
2854 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3217 5013 5411 5685 5832 6243 3217
Size: 1796 398 274 147 411 3554
Fragments arranged by size:
3554 1796 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6355
Fragments arranged by size:
6355 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2949 3608 3674 1186
Size: 1763 659 66 4092
Fragments arranged by size:
4092 1763 659 66
BseYI C'CCAG_C
Cuts at: 2028 3205 3373 3481 5783 6279 2028
Size: 1177 168 108 2302 496 2329
Fragments arranged by size:
2329 2302 1177 496 168 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6007
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4030 4791 5819 1540
Size: 275 186 2029 761 1028 2301
Fragments arranged by size:
2301 2029 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 4641 4726 5797 2043
Size: 2598 85 1071 2826
Fragments arranged by size:
2826 2598 1071 85
BsmI GAATG_Cn'
Cuts at: 3792 4091 4184 3792
Size: 299 93 6188
Fragments arranged by size:
6188 299 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2043 3575 3666 3899 4641 4726
Size: 399 246 7 1532 91 233 742 85
Cuts at: 4726 5797 1391
Size: 1071 2174
Fragments arranged by size:
2174 1532 1071 742 399 246 233 91
85 7
BspEI T'CCGG_A
Cuts at: 6243 6243
Size: 6580
BspHI T'CATG_A
Cuts at: 3144 4469 6199 3144
Size: 1325 1730 3525
Fragments arranged by size:
3525 1730 1325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2731 3935 844
Size: 1887 1204 3489
Fragments arranged by size:
3489 1887 1204
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 4467 1394
Size: 429 1690 954 3507
Fragments arranged by size:
3507 1690 954 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5075 5249 65
Size: 1183 3827 174 1396
Fragments arranged by size:
3827 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3474 3504 5224 5411 1700
Size: 110 1664 30 1720 187 2869
Fragments arranged by size:
2869 1720 1664 187 110 30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6580
BssHII G'CGCG_C
Cuts at: 1925 3500 1925
Size: 1575 5005
BssSI C'ACGA_G
Cuts at: 4640 5652 4640
Size: 1012 5568
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6580
BstBI TT'CG_AA
Cuts at: 2007 5384 2007
Size: 3377 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6580
BstYI r'GATC_y
Cuts at: 1972 3910 4277 4662 4679 6131 6217 6229 6575
Size: 1938 367 385 17 1452 86 12 346
Cuts at: 6575 1972
Size: 1977
Fragments arranged by size:
1977 1938 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2223 2223
Size: 6580
BtgI C'CryG_G
Cuts at: 513 1065 3630 513
Size: 552 2565 3463
Fragments arranged by size:
3463 2565 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3792 487
Size: 683 56 2566 3275
Fragments arranged by size:
3275 2566 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 4096 4890 4910 3166
Size: 930 794 20 4836
Fragments arranged by size:
4836 930 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2944 2979 3619 3654 529
Size: 35 2380 35 640 35 3455
Fragments arranged by size:
3455 2380 640 35 35 35
DraI TTT'AAA
Cuts at: 3884 3884
Size: 6580
DraIII CAC_nnn'GTG
Cuts at: 2079 2079
Size: 6580
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2510 5587 809
Size: 507 1194 3077 1802
Fragments arranged by size:
3077 1802 1194 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2380 2987 3627
Size: 54 1425 50 275 18 550 607 640
Cuts at: 3627 4027 4916 6545 8
Size: 400 889 1629 43
Fragments arranged by size:
1629 1425 889 640 607 550 400 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4027 1537
Size: 275 2215 4090
Fragments arranged by size:
4090 2215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3807 4510 1353
Size: 2454 703 3423
Fragments arranged by size:
3423 2454 703
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2480 5167 5541 5687 387
Size: 1064 138 891 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 891 374 146 138
EcoICRI GAG'CTC
Cuts at: 2041 2041
Size: 6580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2588 2880 3157 4643 5240 6027 6295
Size: 393 956 292 277 1486 597 787 268
Cuts at: 6295 1239
Size: 1524
Fragments arranged by size:
1524 1486 956 787 597 393 292 277
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3675 3675
Size: 6580
EcoRI G'AATT_C
Cuts at: 3889 4267 3889
Size: 378 6202
FspI TGC'GCA
Cuts at: 5086 5086
Size: 6580
HaeII r_GCGC'y
Cuts at: 1678 5727 1678
Size: 4049 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 2579 2611 5221
Size: 32 135 32 776 32 199 32 2610
Cuts at: 5221 5253 5295 5327 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2610 776 199 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3547 3924 4170 669
Size: 988 281 1609 377 246 3079
Fragments arranged by size:
3079 1609 988 377 281 246
HindIII A'AGCT_T
Cuts at: 748 2235 2832 3008 748
Size: 1487 597 176 4320
Fragments arranged by size:
4320 1487 597 176
HpaI GTT'AAC
Cuts at: 4170 4170
Size: 6580
KpnI G_GTAC'C
Cuts at: 3905 3905
Size: 6580
MfeI C'AATT_G
Cuts at: 2796 4179 2796
Size: 1383 5197
MluI A'CGCG_T
Cuts at: 6456 6456
Size: 6580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2271 5694 5878 1305
Size: 966 3423 184 2007
Fragments arranged by size:
3423 2007 966 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3629 10
Size: 54 1425 343 1797 2961
Fragments arranged by size:
2961 1797 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4538 4897 5056 518
Size: 648 3372 359 159 2042
Fragments arranged by size:
3372 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2821 3996 4673 5821 6066 1606
Size: 1215 1175 677 1148 245 2120
Fragments arranged by size:
2120 1215 1175 1148 677 245
NaeI GCC'GGC
Cuts at: 3476 3506 3476
Size: 30 6550
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6028
NdeI CA'TA_TG
Cuts at: 387 2722 3835 387
Size: 2335 1113 3132
Fragments arranged by size:
3132 2335 1113
NgoMIV G'CCGG_C
Cuts at: 3474 3504 3474
Size: 30 6550
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6580
NotI GC'GGCC_GC
Cuts at: 4027 4027
Size: 6580
NspI r_CATG'y
Cuts at: 1132 1229 3938 5483 1132
Size: 97 2709 1545 2229
Fragments arranged by size:
2709 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6580
PciI A'CATG_T
Cuts at: 5479 5479
Size: 6580
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 2756 1108
Size: 920 728 4932
Fragments arranged by size:
4932 920 728
PfoI T'CCnGG_A
Cuts at: 2857 2857
Size: 6580
PmeI GTTT'AAAC
Cuts at: 3884 3884
Size: 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 6162 6194 4649
Size: 32 1481 32 5035
Fragments arranged by size:
5035 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6580
PsiI TTA'TAA
Cuts at: 2838 4150 2838
Size: 1312 5268
PstI C_TGCA'G
Cuts at: 830 3267 3932 830
Size: 2437 665 3478
Fragments arranged by size:
3478 2437 665
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6580
PvuII CAG'CTG
Cuts at: 2821 2821
Size: 6580
SacI G_AGCT'C
Cuts at: 2043 2043
Size: 6580
SalI G'TCGA_C
Cuts at: 1936 3922 1936
Size: 1986 4594
SbfI CC_TGCA'GG
Cuts at: 3932 3932
Size: 6580
ScaI AGT'ACT
Cuts at: 2483 4828 6540 2483
Size: 2345 1712 2523
Fragments arranged by size:
2523 2345 1712
SfcI C'TryA_G
Cuts at: 826 1045 3263 3928 5063 5744 5935 826
Size: 219 2218 665 1135 681 191 1471
Fragments arranged by size:
2218 1471 1135 681 665 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6580
SmaI CCC'GGG
Cuts at: 1872 3907 1872
Size: 2035 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 3200 4684 5585 5847 820
Size: 197 926 153 1104 1484 901 262 1553
Fragments arranged by size:
1553 1484 1104 926 901 262 197 153
SnaBI TAC'GTA
Cuts at: 493 3877 4371 493
Size: 3384 494 2702
Fragments arranged by size:
3384 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6580
SphI G_CATG'C
Cuts at: 1132 3938 1132
Size: 2806 3774
SspI AAT'ATT
Cuts at: 5 52 2633 4504 5
Size: 47 2581 1871 2081
Fragments arranged by size:
2581 2081 1871 47
StuI AGG'CCT
Cuts at: 1517 3452 1517
Size: 1935 4645
StyI C'CwwG_G
Cuts at: 513 1065 4019 513
Size: 552 2954 3074
Fragments arranged by size:
3074 2954 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3197 3553 4607 4624 4777 4962 5407
Size: 1255 1617 356 1054 17 153 185 445
Cuts at: 5407 325
Size: 1498
Fragments arranged by size:
1617 1498 1255 1054 445 356 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2481 4826 6538
Size: 274 80 33 51 1443 504 2345 1712
Cuts at: 6538 96
Size: 138
Fragments arranged by size:
2345 1712 1443 504 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6540 6572 6540
Size: 32 6548
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6580
XbaI T'CTAG_A
Cuts at: 3916 3916
Size: 6580
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6580
XmaI C'CCGG_G
Cuts at: 1870 3905 1870
Size: 2035 4545
XmnI GAAnn'nnTTC
Cuts at: 3727 4709 3727
Size: 982 5598
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4388 276
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI Bsu36I EcoO109I EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI