Restriction map for
pFN21ASDA0547
(Circular) (Six-base) MAPSORT of: pFN21ASDA0547.seq Check: 9617 from: 1 to: 6787
pFN21ASDA0547 6787 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4597 278
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3753 4130 1937
Size: 1816 377 4594
Fragments arranged by size:
4594 1816 377
Acc65I G'GTAC_C
Cuts at: 4022 4108 4022
Size: 86 6701
AclI AA'CG_TT
Cuts at: 1563 4914 5287 1563
Size: 3351 373 3063
Fragments arranged by size:
3351 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2945 3018 3107 3636 3990 4850 6234 6502
Size: 1313 73 89 529 354 860 1384 268
Cuts at: 6502 1632
Size: 1917
Fragments arranged by size:
1917 1384 1313 860 529 354 268 89
73
AflII C'TTAA_G
Cuts at: 820 1017 4066 820
Size: 197 3049 3541
Fragments arranged by size:
3541 3049 197
AflIII A'CryG_T
Cuts at: 1203 5686 6663 1203
Size: 4483 977 1327
Fragments arranged by size:
4483 1327 977
AgeI A'CCGG_T
Cuts at: 5618 5618
Size: 6787
AhdI GACnn_n'nnGTC
Cuts at: 5516 5516
Size: 6787
AleI CACnn'nnGTG
Cuts at: 1166 2984 1166
Size: 1818 4969
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2503 1184
Size: 1319 5468
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 6755
AlwNI CAG_nnn'CTG
Cuts at: 3639 3985 4530 6102 3639
Size: 346 545 1572 4324
Fragments arranged by size:
4324 1572 545 346
ApaI G_GGCC'C
Cuts at: 2030 2030
Size: 6787
ApaLI G'TGCA_C
Cuts at: 4844 6000 4844
Size: 1156 5631
ApoI r'AATT_y
Cuts at: 1493 2840 2906 4096 4322 4474 6583 1493
Size: 1347 66 1190 226 152 2109 1697
Fragments arranged by size:
2109 1697 1347 1190 226 152 66
AscI GG'CGCG_CC
Cuts at: 2159 2247 2159
Size: 88 6699
AseI AT'TA_AT
Cuts at: 160 1032 3935 5341 160
Size: 872 2903 1406 1606
Fragments arranged by size:
2903 1606 1406 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
AvaI C'yCGr_G
Cuts at: 1870 1943 2340 2603 3677 4112 1870
Size: 73 397 263 1074 435 4545
Fragments arranged by size:
4545 1074 435 397 263 73
BamHI G'GATC_C
Cuts at: 3591 4117 4484 3591
Size: 526 367 5894
Fragments arranged by size:
5894 526 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2540 2787 2822 4022 4108
Size: 325 217 627 753 247 35 1200 86
Cuts at: 4108 5563 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1200 753 627 325 247 217
86 35
BanII G_rGCy'C
Cuts at: 1391 2030 2454 2553 3122 3661 3896 4106 1391
Size: 639 424 99 569 539 235 210 4072
Fragments arranged by size:
4072 639 569 539 424 235 210 99
BbeI G_GCGC'C
Cuts at: 2544 2791 2544
Size: 247 6540
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3103 928
Size: 971 1204 4612
Fragments arranged by size:
4612 1204 971
BbvCI CC'TCA_GC
Cuts at: 2087 2087
Size: 6787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2708 2742 4978 5012 1903
Size: 34 771 34 2236 34 3678
Fragments arranged by size:
3678 2236 771 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3968 4679 5889 3968
Size: 711 1210 4866
Fragments arranged by size:
4866 1210 711
BclI T'GATC_A
Cuts at: 1548 3870 1548
Size: 2322 4465
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3237 5398 136
Size: 107 122 71 631 605 1565 2161 1525
Fragments arranged by size:
2161 1565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2274 6782 2274
Size: 4508 2279
BlpI GC'TnA_GC
Cuts at: 4204 4204
Size: 6787
Bme1580I G_kGCm'C
Cuts at: 1790 2030 4848 6004 1790
Size: 240 2818 1156 2573
Fragments arranged by size:
2818 2573 1156 240
BmgBI CAC'GTC
Cuts at: 1318 2374 2773 1318
Size: 1056 399 5332
Fragments arranged by size:
5332 1056 399
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3332 3590 4518
Size: 416 53 83 399 24 1915 258 928
Cuts at: 4518 5476 442
Size: 958 1753
Fragments arranged by size:
1915 1753 958 928 416 399 258 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6787
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 2987
Size: 6787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2777 3273 3347 3690 3879 5447 1239
Size: 1262 276 496 74 343 189 1568 2579
Fragments arranged by size:
2579 1568 1262 496 343 276 189 74
Bpu10I CC'TnA_GC
Cuts at: 2087 2087
Size: 6787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2676 3136 3184 4912 5777 6075 2676
Size: 460 48 1728 865 298 3388
Fragments arranged by size:
3388 1728 865 460 298 48
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3680 3712 5450 882
Size: 617 2181 32 1738 2219
Fragments arranged by size:
2219 2181 1738 617 32
BsaAI yAC'GTr
Cuts at: 493 1204 2119 4084 4578 493
Size: 711 915 1965 494 2702
Fragments arranged by size:
2702 1965 915 711 494
BsaBI GATnn'nnATC
Cuts at: 6781 6781
Size: 6787
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2408 2541 2697 2788
Size: 53 83 186 936 875 133 156 91
Cuts at: 2788 3271 4594 4976 5611 275
Size: 483 1323 382 635 1451
Fragments arranged by size:
1451 1323 936 875 635 483 382 186
156 133 91 83 53
BsaWI w'CCGG_w
Cuts at: 2368 5220 5618 5892 6039 6450 2368
Size: 2852 398 274 147 411 2705
Fragments arranged by size:
2852 2705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 2957 2987 3852
Size: 30 165 30 1038 30 486 30 865
Cuts at: 3852 3882 1178
Size: 30 4083
Fragments arranged by size:
4083 1038 865 486 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 2927 3704 3838 1186
Size: 1357 384 777 134 4135
Fragments arranged by size:
4135 1357 777 384 134
BseYI C'CCAG_C
Cuts at: 2643 4073 5990 6486 2643
Size: 1430 1917 496 2944
Fragments arranged by size:
2944 1917 1430 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2141 3159 3625 1163
Size: 573 405 1018 466 4325
Fragments arranged by size:
4325 1018 573 466 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4237 4998 6026 1540
Size: 275 186 2236 761 1028 2301
Fragments arranged by size:
2301 2236 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3711 3804 3896 4848 4933 6004 3711
Size: 93 92 952 85 1071 4494
Fragments arranged by size:
4494 1071 952 93 92 85
BsmI GAATG_Cn'
Cuts at: 3466 3625 4298 4391 3466
Size: 159 673 93 5862
Fragments arranged by size:
5862 673 159 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2399 2399
Size: 6787
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2454 2553 3122 3661 3711 3804
Size: 399 240 424 99 569 539 50 93
Cuts at: 3804 3896 4106 4848 4933 6004 1391
Size: 92 210 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 539 424 399 240
210 99 93 92 85 50
BspEI T'CCGG_A
Cuts at: 2368 6450 2368
Size: 4082 2705
BspHI T'CATG_A
Cuts at: 4676 6406 4676
Size: 1730 5057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4142 844
Size: 3298 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2173 4674 1394
Size: 429 350 2501 3507
Fragments arranged by size:
3507 2501 429 350
BsrDI GCAATG_nn'
Cuts at: 65 1248 5282 5456 65
Size: 1183 4034 174 1396
Fragments arranged by size:
4034 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2874 5431 5618 1700
Size: 110 1064 2557 187 2869
Fragments arranged by size:
2869 2557 1064 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6787
BssHII G'CGCG_C
Cuts at: 1925 2108 2159 2224 2247 2268 1925
Size: 183 51 65 23 21 6444
Fragments arranged by size:
6444 183 65 51 23 21
BssSI C'ACGA_G
Cuts at: 4847 5859 4847
Size: 1012 5775
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2221 794
Size: 1427 5360
BstBI TT'CG_AA
Cuts at: 2007 5591 2007
Size: 3584 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6787
BstYI r'GATC_y
Cuts at: 1972 2274 3591 3831 4117 4484 4869 4886 6338
Size: 302 1317 240 286 367 385 17 1452
Cuts at: 6338 6424 6436 6782 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1317 385 367 346 302 286
240 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2325 2544 2619 2826 3926 513
Size: 552 957 303 219 75 207 1100 3374
Fragments arranged by size:
3374 1100 957 552 303 219 207 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2614 487
Size: 683 56 1388 4660
Fragments arranged by size:
4660 1388 683 56
BtsI GCAGTG_nn'
Cuts at: 2215 2848 3169 4303 5097 5117 2215
Size: 633 321 1134 794 20 3885
Fragments arranged by size:
3885 1134 794 633 321 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6787
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6752
DraI TTT'AAA
Cuts at: 4091 4091
Size: 6787
DraIII CAC_nnn'GTG
Cuts at: 2221 3453 2221
Size: 1232 5555
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5794 809
Size: 507 4478 1802
Fragments arranged by size:
4478 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2580 2704
Size: 54 1425 50 275 18 273 477 124
Cuts at: 2704 3097 3430 3929 4234 5123 6752 8
Size: 393 333 499 305 889 1629 43
Fragments arranged by size:
1629 1425 889 499 477 393 333 305
275 273 124 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4234 1537
Size: 275 2422 4090
Fragments arranged by size:
4090 2422 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3083 3966 4717 1353
Size: 1730 883 751 3423
Fragments arranged by size:
3423 1730 883 751
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5374 5748 5894 387
Size: 1064 138 3785 374 146 1280
Fragments arranged by size:
3785 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3894 3894
Size: 6787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 2777 2945 3018 3107 3273 3347
Size: 393 869 276 168 73 89 166 74
Cuts at: 3347 3636 3690 3879 3990 4850 5447 6234 6502
Size: 289 54 189 111 860 597 787 268
Cuts at: 6502 1239
Size: 1524
Fragments arranged by size:
1524 869 860 787 597 393 289 276
268 189 168 166 111 89 74 73
54
EcoNI CCTnn'n_nnAGG
Cuts at: 2582 2582
Size: 6787
EcoO109I rG'GnC_Cy
Cuts at: 2027 2125 2533 3076 3403 2027
Size: 98 408 543 327 5411
Fragments arranged by size:
5411 543 408 327 98
EcoRI G'AATT_C
Cuts at: 2840 4096 4474 2840
Size: 1256 378 5153
Fragments arranged by size:
5153 1256 378
FspI TGC'GCA
Cuts at: 5293 5293
Size: 6787
HaeII r_GCGC'y
Cuts at: 1678 2085 2292 2544 2791 5934 1678
Size: 407 207 252 247 3143 2531
Fragments arranged by size:
3143 2531 407 252 247 207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2266 2298 2441 2473 2546
Size: 32 135 32 694 32 143 32 73
Cuts at: 2546 2578 2674 2706 2957 2989 3197 3229 5428
Size: 32 96 32 251 32 208 32 2199
Cuts at: 5428 5460 5502 5534 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2199 694 251 208 143 135 96
73 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2427 4131 4377 669
Size: 988 281 489 1704 246 3079
Fragments arranged by size:
3079 1704 988 489 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6787
HpaI GTT'AAC
Cuts at: 2427 4377 2427
Size: 1950 4837
KasI G'GCGC_C
Cuts at: 2540 2787 2540
Size: 247 6540
KpnI G_GTAC'C
Cuts at: 4026 4112 4026
Size: 86 6701
MfeI C'AATT_G
Cuts at: 4386 4386
Size: 6787
MluI A'CGCG_T
Cuts at: 6663 6663
Size: 6787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3805 5901 6085 1305
Size: 2500 2096 184 2007
Fragments arranged by size:
2500 2096 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2706 3099 3931 10
Size: 54 1425 343 874 393 832 2866
Fragments arranged by size:
2866 1425 874 832 393 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2984 4745 5104 5263 518
Size: 648 1818 1761 359 159 2042
Fragments arranged by size:
2042 1818 1761 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2327 2546 2621 2785 4203 4880 6028 6273
Size: 721 219 75 164 1418 677 1148 245
Cuts at: 6273 1606
Size: 2120
Fragments arranged by size:
2120 1418 1148 721 677 245 219 164
75
NaeI GCC'GGC
Cuts at: 2876 2876
Size: 6787
NarI GG'CG_CC
Cuts at: 2541 2788 2541
Size: 247 6540
NcoI C'CATG_G
Cuts at: 513 1065 2022 3926 513
Size: 552 957 1904 3374
Fragments arranged by size:
3374 1904 957 552
NdeI CA'TA_TG
Cuts at: 387 3919 387
Size: 3532 3255
NgoMIV G'CCGG_C
Cuts at: 2874 2874
Size: 6787
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6787
NotI GC'GGCC_GC
Cuts at: 4234 4234
Size: 6787
NspI r_CATG'y
Cuts at: 1132 1229 4145 5690 1132
Size: 97 2916 1545 2229
Fragments arranged by size:
2916 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2536 1348
Size: 1188 5599
PciI A'CATG_T
Cuts at: 5686 5686
Size: 6787
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2524 1108
Size: 1416 5371
PfoI T'CCnGG_A
Cuts at: 3856 3856
Size: 6787
PmeI GTTT'AAAC
Cuts at: 4091 4091
Size: 6787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4856 4888 6369 6401 4856
Size: 32 1481 32 5242
Fragments arranged by size:
5242 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 3076 3403 2125
Size: 951 327 5509
Fragments arranged by size:
5509 951 327
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6787
PsiI TTA'TAA
Cuts at: 4357 4357
Size: 6787
PspOMI G'GGCC_C
Cuts at: 2026 2026
Size: 6787
PspXI vC'TCGA_Gb
Cuts at: 2603 2603
Size: 6787
PstI C_TGCA'G
Cuts at: 830 2350 4139 830
Size: 1520 1789 3478
Fragments arranged by size:
3478 1789 1520
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6787
SacI G_AGCT'C
Cuts at: 3896 3896
Size: 6787
SacII CC_GC'GG
Cuts at: 2328 2547 2622 2328
Size: 219 75 6493
Fragments arranged by size:
6493 219 75
SalI G'TCGA_C
Cuts at: 1936 4129 1936
Size: 2193 4594
SapI GCTCTTCn'nnn_
Cuts at: 3966 3966
Size: 6787
SbfI CC_TGCA'GG
Cuts at: 4139 4139
Size: 6787
ScaI AGT'ACT
Cuts at: 5035 6747 5035
Size: 1712 5075
SfcI C'TryA_G
Cuts at: 826 1045 2346 3882 4135 5270 5951 6142 826
Size: 219 1301 1536 253 1135 681 191 1471
Fragments arranged by size:
1536 1471 1301 1135 681 253 219 191
SfoI GGC'GCC
Cuts at: 2542 2789 2542
Size: 247 6540
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
SgrAI Cr'CCGG_yG
Cuts at: 1700 2874 1700
Size: 1174 5613
SmaI CCC'GGG
Cuts at: 1872 2342 4114 1872
Size: 470 1772 4545
Fragments arranged by size:
4545 1772 470
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2603 2691 3115 3199 4066 4891
Size: 197 926 660 88 424 84 867 825
Cuts at: 4891 5792 6054 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 867 825 660 424 262
197 88 84
SnaBI TAC'GTA
Cuts at: 493 4084 4578 493
Size: 3591 494 2702
Fragments arranged by size:
3591 2702 494
SpeI A'CTAG_T
Cuts at: 152 3953 152
Size: 3801 2986
SphI G_CATG'C
Cuts at: 1132 4145 1132
Size: 3013 3774
SrfI GCCC'GGGC
Cuts at: 2342 2342
Size: 6787
SspI AAT'ATT
Cuts at: 5 52 2890 3503 4711 5
Size: 47 2838 613 1208 2081
Fragments arranged by size:
2838 2081 1208 613 47
StuI AGG'CCT
Cuts at: 1517 2689 1517
Size: 1172 5615
StyI C'CwwG_G
Cuts at: 513 1065 2022 3351 3776 3926 3991 4226 513
Size: 552 957 1329 425 150 65 235 3074
Fragments arranged by size:
3074 1329 957 552 425 235 150 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2581 4814 4831 4984 5169 5614 325
Size: 1255 1001 2233 17 153 185 445 1498
Fragments arranged by size:
2233 1498 1255 1001 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3541 5033 6745
Size: 274 80 33 51 1443 1564 1492 1712
Cuts at: 6745 96
Size: 138
Fragments arranged by size:
1712 1564 1492 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3852 3884 6747 6779 3852
Size: 32 2863 32 3860
Fragments arranged by size:
3860 2863 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6787
XbaI T'CTAG_A
Cuts at: 4123 4123
Size: 6787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2575 2575
Size: 6787
XhoI C'TCGA_G
Cuts at: 1943 2603 1943
Size: 660 6127
XmaI C'CCGG_G
Cuts at: 1870 2340 4112 1870
Size: 470 1772 4545
Fragments arranged by size:
4545 1772 470
XmnI GAAnn'nnTTC
Cuts at: 4916 4916
Size: 6787
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4595 276
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PsrI PvuII RsrII SanDI SexAI SfiI SwaI