Restriction map for
pFN21ASDA0535
(Circular) (Six-base) MAPSORT of: pFN21ASDA0535.seq Check: 3542 from: 1 to: 7870
pFN21ASDA0535 7870 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5680 278
Size: 53 83 186 936 4144 2468
Fragments arranged by size:
4144 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5213 1937
Size: 3276 4594
Acc65I G'GTAC_C
Cuts at: 5191 5191
Size: 7870
AclI AA'CG_TT
Cuts at: 1563 5997 6370 1563
Size: 4434 373 3063
Fragments arranged by size:
4434 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2058 2169 2757 4761 4856 5933 7317 7585
Size: 426 111 588 2004 95 1077 1384 268
Cuts at: 7585 1632
Size: 1917
Fragments arranged by size:
2004 1917 1384 1077 588 426 268 111
95
AflII C'TTAA_G
Cuts at: 820 1017 3868 4319 820
Size: 197 2851 451 4371
Fragments arranged by size:
4371 2851 451 197
AflIII A'CryG_T
Cuts at: 1203 3154 6769 7746 1203
Size: 1951 3615 977 1327
Fragments arranged by size:
3615 1951 1327 977
AgeI A'CCGG_T
Cuts at: 6701 6701
Size: 7870
AhdI GACnn_n'nnGTC
Cuts at: 6599 6599
Size: 7870
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2536 2967 1184
Size: 1352 431 6087
Fragments arranged by size:
6087 1352 431
AlwNI CAG_nnn'CTG
Cuts at: 2036 3746 3749 3817 4535 5613 7185 2036
Size: 1710 3 68 718 1078 1572 2721
Fragments arranged by size:
2721 1710 1572 1078 718 68 3
ApaLI G'TGCA_C
Cuts at: 5927 7083 5927
Size: 1156 6714
ApoI r'AATT_y
Cuts at: 1493 2426 2464 2747 2926 3225 3446 3641 4099
Size: 933 38 283 179 299 221 195 458
Cuts at: 4099 5044 5179 5405 5557 7666 1493
Size: 945 135 226 152 2109 1697
Fragments arranged by size:
2109 1697 945 933 458 299 283 226
221 195 179 152 135 38
AseI AT'TA_AT
Cuts at: 160 1032 6424 160
Size: 872 5392 1606
Fragments arranged by size:
5392 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7870
AvaI C'yCGr_G
Cuts at: 1870 1943 4348 5195 1870
Size: 73 2405 847 4545
Fragments arranged by size:
4545 2405 847 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2368 2401 2368
Size: 33 7837
BamHI G'GATC_C
Cuts at: 5200 5567 5200
Size: 367 7503
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 3195 3668 5191 6646
Size: 325 217 627 290 1118 473 1523 1455
Cuts at: 6646 618
Size: 1842
Fragments arranged by size:
1842 1523 1455 1118 627 473 325 290
217
BanII G_rGCy'C
Cuts at: 1391 2106 2397 3168 3686 4091 5189 1391
Size: 715 291 771 518 405 1098 4072
Fragments arranged by size:
4072 1098 771 715 518 405 291
BbeI G_GCGC'C
Cuts at: 3672 3672
Size: 7870
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2244 2324 2376 3076 3732 4061 4458
Size: 971 345 80 52 700 656 329 397
Cuts at: 4458 928
Size: 4340
Fragments arranged by size:
4340 971 700 656 397 345 329 80
52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6061 6095 1903
Size: 34 4124 34 3678
Fragments arranged by size:
4124 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5165 5762 6972 5165
Size: 597 1210 6063
Fragments arranged by size:
6063 1210 597
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7870
BfrBI ATG'CAT
Cuts at: 3238 4241 3238
Size: 1003 6867
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6481 136
Size: 107 122 71 631 605 4809 1525
Fragments arranged by size:
4809 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4168 7865 4168
Size: 3697 4173
BlpI GC'TnA_GC
Cuts at: 2964 5287 2964
Size: 2323 5547
Bme1580I G_kGCm'C
Cuts at: 1790 3128 3200 4356 5931 7087 1790
Size: 1338 72 1156 1575 1156 2573
Fragments arranged by size:
2573 1575 1338 1156 1156 72
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7870
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4076 4782
Size: 416 53 83 399 24 1988 671 706
Cuts at: 4782 5601 6559 442
Size: 819 958 1753
Fragments arranged by size:
1988 1753 958 819 706 671 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7870
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4607
Size: 7870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2341 2960 3038 4143 6530 1239
Size: 1102 619 78 1105 2387 2579
Fragments arranged by size:
2579 2387 1105 1102 619 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3236 5995 6860 7158 3236
Size: 2759 865 298 3948
Fragments arranged by size:
3948 2759 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3110 6533 882
Size: 617 1611 3423 2219
Fragments arranged by size:
3423 2219 1611 617
BsaAI yAC'GTr
Cuts at: 493 1204 3157 4225 4357 5167 5661 493
Size: 711 1953 1068 132 810 494 2702
Fragments arranged by size:
2702 1953 1068 810 711 494 132
BsaBI GATnn'nnATC
Cuts at: 3372 7864 3372
Size: 4492 3378
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3669 5677 6059 6694
Size: 53 83 186 936 2136 2008 382 635
Cuts at: 6694 275
Size: 1451
Fragments arranged by size:
2136 2008 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4391 6303 6701 6975 7122 7533 4391
Size: 1912 398 274 147 411 4728
Fragments arranged by size:
4728 1912 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2624 2654 3107 3137 3782
Size: 30 165 30 1221 30 453 30 645
Cuts at: 3782 3812 4307 4337 1178
Size: 30 495 30 4711
Fragments arranged by size:
4711 1221 645 495 453 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2575 2873 4076 4079 4931 4934 1186
Size: 1389 298 1203 3 852 3 4122
Fragments arranged by size:
4122 1389 1203 852 298 3 3
BseYI C'CCAG_C
Cuts at: 2628 2835 4017 4651 7073 7569 2628
Size: 207 1182 634 2422 496 2929
Fragments arranged by size:
2929 2422 1182 634 496 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2328 2637 3684 3888 1163
Size: 573 592 309 1047 204 5145
Fragments arranged by size:
5145 1047 592 573 309 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5320 6081 7109 1540
Size: 275 186 3319 761 1028 2301
Fragments arranged by size:
3319 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2397 3024 3252 4091 5931 6016 7087 2106
Size: 291 627 228 839 1840 85 1071 2889
Fragments arranged by size:
2889 1840 1071 839 627 291 228 85
BsmI GAATG_Cn'
Cuts at: 2809 3969 3986 5117 5381 5474 2809
Size: 1160 17 1131 264 93 5205
Fragments arranged by size:
5205 1160 1131 264 93 17
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2397 3024 3128 3168 3200 3252
Size: 399 316 291 627 104 40 32 52
Cuts at: 3252 3686 4091 4356 5189 5931 6016 7087 1391
Size: 434 405 265 833 742 85 1071 2174
Fragments arranged by size:
2174 1071 833 742 627 434 405 399
316 291 265 104 85 52 40 32
BspEI T'CCGG_A
Cuts at: 4391 7533 4391
Size: 3142 4728
BspHI T'CATG_A
Cuts at: 2307 4749 5759 7489 2307
Size: 2442 1010 1730 2688
Fragments arranged by size:
2688 2442 1730 1010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4961 5225 844
Size: 4117 264 3489
Fragments arranged by size:
4117 3489 264
BsrBI CCG'CTC
Cuts at: 1394 1823 5757 1394
Size: 429 3934 3507
Fragments arranged by size:
3934 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2836 3363 4651 6365 6539 65
Size: 1183 1588 527 1288 1714 174 1396
Fragments arranged by size:
1714 1588 1396 1288 1183 527 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6514 6701 1700
Size: 110 4704 187 2869
Fragments arranged by size:
4704 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5079 96
Size: 4983 2887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7870
BssSI C'ACGA_G
Cuts at: 4401 5930 6942 4401
Size: 1529 1012 5329
Fragments arranged by size:
5329 1529 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3746 3749 794
Size: 2952 3 4915
Fragments arranged by size:
4915 2952 3
BstBI TT'CG_AA
Cuts at: 2007 6674 2007
Size: 4667 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7870
BstXI CCAn_nnnn'nTGG
Cuts at: 3686 3686
Size: 7870
BstYI r'GATC_y
Cuts at: 1972 4168 5200 5567 5952 5969 7421 7507 7519
Size: 2196 1032 367 385 17 1452 86 12
Cuts at: 7519 7865 1972
Size: 346 1977
Fragments arranged by size:
2196 1977 1452 1032 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2127 513
Size: 552 957 105 6256
Fragments arranged by size:
6256 957 552 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2520 4232 4356 487
Size: 683 56 1294 1712 124 4001
Fragments arranged by size:
4001 1712 1294 683 124 56
BtsI GCAGTG_nn'
Cuts at: 2251 3407 4258 5151 5386 6180 6200 2251
Size: 1156 851 893 235 794 20 3921
Fragments arranged by size:
3921 1156 893 851 794 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7870
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7835
DraI TTT'AAA
Cuts at: 2469 2493 5174 2469
Size: 24 2681 5165
Fragments arranged by size:
5165 2681 24
DraIII CAC_nnn'GTG
Cuts at: 3208 3208
Size: 7870
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6877 809
Size: 507 5561 1802
Fragments arranged by size:
5561 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3688 4219 4489
Size: 54 1425 50 275 18 1858 531 270
Cuts at: 4489 5317 6206 7835 8
Size: 828 889 1629 43
Fragments arranged by size:
1858 1629 1425 889 828 531 275 270
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5317 1537
Size: 275 3505 4090
Fragments arranged by size:
4090 3505 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2132 2542 2889 3952 5800 1353
Size: 779 410 347 1063 1848 3423
Fragments arranged by size:
3423 1848 1063 779 410 347
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6457 6831 6977 387
Size: 1064 138 4868 374 146 1280
Fragments arranged by size:
4868 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2104 2395 4089 2104
Size: 291 1694 5885
Fragments arranged by size:
5885 1694 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2169 2341 2757 2960 3038 4143
Size: 393 426 111 172 416 203 78 1105
Cuts at: 4143 4761 4856 5933 6530 7317 7585 1239
Size: 618 95 1077 597 787 268 1524
Fragments arranged by size:
1524 1105 1077 787 618 597 426 416
393 268 203 172 111 95 78
EcoNI CCTnn'n_nnAGG
Cuts at: 2912 3663 4476 2912
Size: 751 813 6306
Fragments arranged by size:
6306 813 751
EcoO109I rG'GnC_Cy
Cuts at: 5027 5027
Size: 7870
EcoRI G'AATT_C
Cuts at: 3446 5179 5557 3446
Size: 1733 378 5759
Fragments arranged by size:
5759 1733 378
FspI TGC'GCA
Cuts at: 6376 6376
Size: 7870
HaeII r_GCGC'y
Cuts at: 1678 3672 7017 1678
Size: 1994 3345 2531
Fragments arranged by size:
3345 2531 1994
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 2624 2656 3782
Size: 32 135 32 791 32 229 32 1126
Cuts at: 3782 3814 4689 4721 6511 6543 6585 6617 1373
Size: 32 875 32 1790 32 42 32 2626
Fragments arranged by size:
2626 1790 1126 875 791 229 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 5214 5460 669
Size: 988 281 238 3038 246 3079
Fragments arranged by size:
3079 3038 988 281 246 238
HindIII A'AGCT_T
Cuts at: 748 4154 4384 4687 748
Size: 3406 230 303 3931
Fragments arranged by size:
3931 3406 303 230
HpaI GTT'AAC
Cuts at: 5460 5460
Size: 7870
KasI G'GCGC_C
Cuts at: 3668 3668
Size: 7870
KpnI G_GTAC'C
Cuts at: 5195 5195
Size: 7870
MfeI C'AATT_G
Cuts at: 2949 3333 5469 2949
Size: 384 2136 5350
Fragments arranged by size:
5350 2136 384
MluI A'CGCG_T
Cuts at: 7746 7746
Size: 7870
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2667 3825 3959 4220 4352 4863 6984 7168
Size: 1362 1158 134 261 132 511 2121 184
Cuts at: 7168 1305
Size: 2007
Fragments arranged by size:
2121 2007 1362 1158 511 261 184 134
132
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4221 4491 10
Size: 54 1425 343 2389 270 3389
Fragments arranged by size:
3389 2389 1425 343 270 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2538 3243 4204 4251 4809 4889 5075
Size: 648 1372 705 961 47 558 80 186
Cuts at: 5075 5828 6187 6346 518
Size: 753 359 159 2042
Fragments arranged by size:
2042 1372 961 753 705 648 558 359
186 159 80 47
MspA1I CmG'CkG
Cuts at: 1606 2147 3344 3746 3774 5286 5963 7111 7356
Size: 541 1197 402 28 1512 677 1148 245
Cuts at: 7356 1606
Size: 2120
Fragments arranged by size:
2120 1512 1197 1148 677 541 402 245
28
NarI GG'CG_CC
Cuts at: 3669 3669
Size: 7870
NcoI C'CATG_G
Cuts at: 513 1065 2022 2127 513
Size: 552 957 105 6256
Fragments arranged by size:
6256 957 552 105
NdeI CA'TA_TG
Cuts at: 387 5056 387
Size: 4669 3201
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7870
NotI GC'GGCC_GC
Cuts at: 5317 5317
Size: 7870
NsiI A_TGCA'T
Cuts at: 3240 4243 3240
Size: 1003 6867
NspI r_CATG'y
Cuts at: 1132 1229 3824 5228 6773 1132
Size: 97 2595 1404 1545 2229
Fragments arranged by size:
2595 2229 1545 1404 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7870
PciI A'CATG_T
Cuts at: 6769 6769
Size: 7870
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7870
PfoI T'CCnGG_A
Cuts at: 2097 3891 4120 4595 2097
Size: 1794 229 475 5372
Fragments arranged by size:
5372 1794 475 229
PmeI GTTT'AAAC
Cuts at: 5174 5174
Size: 7870
PmlI CAC'GTG
Cuts at: 3157 4225 4357 3157
Size: 1068 132 6670
Fragments arranged by size:
6670 1068 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5939 5971 7452 7484 5939
Size: 32 1481 32 6325
Fragments arranged by size:
6325 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5027 5027
Size: 7870
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7870
PsiI TTA'TAA
Cuts at: 5440 5440
Size: 7870
PstI C_TGCA'G
Cuts at: 830 2961 3343 3779 5222 830
Size: 2131 382 436 1443 3478
Fragments arranged by size:
3478 2131 1443 436 382
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7870
PvuII CAG'CTG
Cuts at: 2147 3344 3746 2147
Size: 1197 402 6271
Fragments arranged by size:
6271 1197 402
SacI G_AGCT'C
Cuts at: 2106 2397 4091 2106
Size: 291 1694 5885
Fragments arranged by size:
5885 1694 291
SalI G'TCGA_C
Cuts at: 1936 5212 1936
Size: 3276 4594
SbfI CC_TGCA'GG
Cuts at: 5222 5222
Size: 7870
ScaI AGT'ACT
Cuts at: 6118 7830 6118
Size: 1712 6158
SfcI C'TryA_G
Cuts at: 826 1045 2212 2957 3339 3775 4036 5218 6353
Size: 219 1167 745 382 436 261 1182 1135
Cuts at: 6353 7034 7225 826
Size: 681 191 1471
Fragments arranged by size:
1471 1182 1167 1135 745 681 436 382
261 219 191
SfoI GGC'GCC
Cuts at: 3670 3670
Size: 7870
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7870
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7870
SmaI CCC'GGG
Cuts at: 1872 5197 1872
Size: 3325 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3251 3868 4319 5974 6875 7137
Size: 197 926 1308 617 451 1655 901 262
Cuts at: 7137 820
Size: 1553
Fragments arranged by size:
1655 1553 1308 926 901 617 451 262
197
SnaBI TAC'GTA
Cuts at: 493 5167 5661 493
Size: 4674 494 2702
Fragments arranged by size:
4674 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7870
SphI G_CATG'C
Cuts at: 1132 3824 5228 1132
Size: 2692 1404 3774
Fragments arranged by size:
3774 2692 1404
SspI AAT'ATT
Cuts at: 5 52 5794 5
Size: 47 5742 2081
Fragments arranged by size:
5742 2081 47
StuI AGG'CCT
Cuts at: 1517 3103 4130 1517
Size: 1586 1027 5257
Fragments arranged by size:
5257 1586 1027
StyI C'CwwG_G
Cuts at: 513 1065 2022 2127 2271 2919 3098 3825 4437
Size: 552 957 105 144 648 179 727 612
Cuts at: 4437 4492 5309 513
Size: 55 817 3074
Fragments arranged by size:
3074 957 817 727 648 612 552 179
144 105 55
SwaI ATTT'AAAT
Cuts at: 2469 2469
Size: 7870
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3053 3323 3692 3746 4694 5897 5914
Size: 1255 1473 270 369 54 948 1203 17
Cuts at: 5914 6067 6252 6697 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1473 1255 1203 948 445 369 270
185 153 54 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5079 6116 7828
Size: 274 80 33 51 1443 3102 1037 1712
Cuts at: 7828 96
Size: 138
Fragments arranged by size:
3102 1712 1443 1037 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2061 2093 2658 2690 3107 3139 3916 3948 4307
Size: 32 565 32 417 32 777 32 359
Cuts at: 4307 4339 4402 4434 7830 7862 2061
Size: 32 63 32 3396 32 2069
Fragments arranged by size:
3396 2069 777 565 417 359 63 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7870
XbaI T'CTAG_A
Cuts at: 4093 5206 4093
Size: 1113 6757
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7870
XmaI C'CCGG_G
Cuts at: 1870 5195 1870
Size: 3325 4545
XmnI GAAnn'nnTTC
Cuts at: 2331 5999 2331
Size: 3668 4202
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5678 276
Size: 53 83 186 936 4144 2468
Fragments arranged by size:
4144 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI
NaeI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SrfI XcmI